@article{2-s2.0-85117591328,

title = {Effect of nickel as stress factor on phenol biodegradation by stenotrophomonas maltophilia kb2},

author = { A. Gąszczak and E. Szczyrba and A. Szczotka and I. Greń},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117591328&doi=10.3390%2fma14206058&partnerID=40&md5=64d8d4b83d269e618b9b3a37930e3c7b},

doi = {10.3390/ma14206058},

issn = {19961944},

year  = {2021},

date = {2021-01-01},

journal = {Materials},

volume = {14},

number = {20},

publisher = {MDPI},

abstract = {This study focuses on the phenol biodegradation kinetics by Stenotrophomonas maltophilia KB2 in a nickel-contaminated medium. Initial tests proved that a nickel concentration of 33.3 mg·L−1 caused a cessation of bacterial growth. The experiments were conducted in a batch bioreactor in several series: without nickel, at constant nickel concentration and at varying metal concentrations (1.67–13.33 g·m−3 ). For a constant Ni2+ concentration (1.67 or 3.33 g·m−3 ), a comparable bacterial growth rate was obtained regardless of the initial phenol concentration (50–300 g·m−3 ). The depen-dence µ = f (S0) at constant Ni2+ concentration was very well described by the Monod equations. The created varying nickel concentrations experimental database was used to estimate the parameters of selected mathematical models, and the analysis included different methods of determining metal inhibition constant KIM. Each model showed a very good fit with the experimental data (R2 values were higher than 0.9). The best agreement (R2 = 0.995) was achieved using a modified Andrews equation, which considers the metal influence and substrate inhibition. Therefore, kinetic equation parameters were estimated: µmax = 1.584 h−1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85092763346,

title = {The communities of ectomycorrhizal fungal species associated with Betula pendula Roth and Pinus sylvestris L. growing in heavy-metal contaminated soils},

author = { W.M. Bierza and K.A. Bierza and A. Trzebny and I. Greń and M. Dabert and R. Ciepał and L.K. Trocha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092763346&doi=10.1007%2fs11104-020-04737-4&partnerID=40&md5=f5ac89886482da3a5b02385c802a8ee6},

doi = {10.1007/s11104-020-04737-4},

issn = {0032079X},

year  = {2020},

date = {2020-01-01},

journal = {Plant and Soil},

volume = {457},

number = {1-2},

pages = {321-338},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {Aims: Pioneer tree species such as Betula pendula and Pinus sylvestris encroach soils contaminated with heavy metals (HMs). This is facilitated by ectomycorrhizal fungi colonizing tree roots. Thus, we evaluated the ectomycorrhizal fungal (EMF) communities of B. pendula and P. sylvestris growing in HM-contaminated soils compared to non-contaminated soils. We also studied the effect of HMs and soil properties on EMF communities and soil fungal biomass. Methods: Roots of B. pendula and P. sylvestris were collected from three HM-contaminated sites and from two non-contaminated sites located in Poland. EMF species were identified using DNA barcoding. Soil fungal biomass was determined by soil ergosterol. Results: B. pendula and P. sylvestris growing in HM-contaminated soils had similar EMF communities, where Scleroderma, Rhizopogon and Russula as well as ectomycorrhizae of the long-distance exploration type dominated. Among all of the examined soil factors studied, toxicity index (TITotal) was the most significant factor shaping the composition of EMF communities. Despite significant differences in the structure of the EMF communities of trees growing in HM-contaminated sites compared to control sites, no differences in overall diversity were observed. Conclusions: Only well-adapted EMF species can survive toxic conditions and form ectomycorrhizal symbiosis with encroaching trees facilitating the forest succession on contaminated soils. © 2020, The Author(s).},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85070258617,

title = {Impact of the biological cotreatment of the Kalina pond leachate on laboratory sequencing batch reactor operation and activated sludge quality},

author = { J. Michalska and I. Greń and J. Żur and D. Wasilkowski and A. Mrozik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070258617&doi=10.3390%2fw11081539&partnerID=40&md5=54915800dfbf65bd693faf9ec666a830},

doi = {10.3390/w11081539},

issn = {20734441},

year  = {2019},

date = {2019-01-01},

journal = {Water (Switzerland)},

volume = {11},

number = {8},

publisher = {MDPI AG},

abstract = {Hauling landfill leachate to offsite urban wastewater treatment plants is a way to achieve pollutant removal. However, the implementation of biological methods for the treatment of landfill leachate can be extremely challenging. This study aims to investigate the effect of blending wastewater with 3.5% and 5.5% of the industrial leachate from the Kalina pond (KPL) on the performance of sequencing batch reactor (SBR) and capacity of activated sludge microorganisms. The results showed that the removal efficiency of the chemical oxygen demand declined in the contaminated SBR from 100% to 69% and, subsequently, to 41% after the cotreatment with 3.5% and 5.5% of the pollutant. In parallel, the activities of the dehydrogenases and nonspecific esterases declined by 58% and 39%, and 79% and 81% after 32 days of the exposure of the SBR to 3.5% and 5.5% of the leachate, respectively. Furthermore, the presence of the KPL in the sewage affected the sludge microorganisms through a reduction in their functional capacity as well as a decrease in the percentages of the marker fatty acids for different microbial groups. A multifactorial analysis of the parameters relevant for the wastewater treatment process confirmed unambiguously the negative impact of the leachate on the operation, activity, and structure of the activated sludge. © 2019 by the authors.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85056272676,

title = {Kinetics of Vinyl Acetate Biodegradation by Pseudomonas fluorescens PCM 2123},

author = { A. Gąszczak and G. Bartelmus and I. Greń and D. Janecki},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056272676&doi=10.1515%2feces-2018-0033&partnerID=40&md5=9fb33cce321371953a6282cc966545ef},

doi = {10.1515/eces-2018-0033},

issn = {18986196},

year  = {2018},

date = {2018-01-01},

journal = {Ecological Chemistry and Engineering S},

volume = {25},

number = {3},

pages = {487-502},

publisher = {Sciendo},

abstract = {The microbial degradation of vinyl acetate (VA) by Pseudomonas fluorescens PCM 2123 strain was studied in both batch and continuous modes. The purpose of the experiments was to determine the kinetic model of the cell growth and biodegradation rate of vinyl acetate (VA), which was the sole carbon and energy source for tested microorganisms. The experiments, carried out in a batch reactor for several initial concentrations of growth substrate in the liquid phase ranging from 18.6 to 373 gsubstrate·m-3 (gs·m-3) made it possible to choose the kinetic model and to estimate its constants. The Haldane inhibitory model with the values of constants: μm = 0.1202 h-1},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85059783087,

title = {Kinetics of cometabolic biodegradation of 4-chlorophenol and phenol by Stenotrophomonas maltophilia KB2},

author = { A. Gąszczak and G. Bartelmus and I. Greń and A. Rotkegel and D. Janecki},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059783087&doi=10.24425%2f122959&partnerID=40&md5=48760f8be66e31d1b82a505e839f8eb3},

doi = {10.24425/122959},

issn = {02086425},

year  = {2018},

date = {2018-01-01},

journal = {Chemical and Process Engineering - Inzynieria Chemiczna i Procesowa},

volume = {39},

number = {4},

pages = {395-410},

publisher = {Polish Academy of Sciences},

abstract = {The cometabolic biodegradation of 4-Chlorophenol (4-CP) by the Stenotrophomonas maltophilia KB2 strain in the presence of phenol (P) was studied. In order to determine the kinetics of biodegradation of both substrates, present alone and in cometabolic systems, a series of tests was carried out in a batch reactor changing, in a wide range, the initial concentration of both substrates. The growth of the tested strain on phenol alone was described by Haldane kinetic model (µ m = 0.9 1/h; K sg = 48.97 g g /m 3 ; K Ig = 256.12 g g /m 3 ; Y xg = 0.5715). The rate of 4-CP transformation by resting cells of KB2 strain was also described by Haldane equation and the estimated parameters of the model were: k c = 0.229 g c /g x h, K sc = 0.696 g c /m 3 , K Ic = 43.82 g c /m 3 . Cometabolic degradation of 4-CP in the presence of phenol was investigated for a wide range of initial 4-CP and phenol concentrations (22–66 g c /m 3 and 67–280 g g /m 3 respectively). The experimental database was exploited to verify the two kinetic models: CIModel taking only the competitive inhibition into consideration and a more universal CNIModel considering both competitive and non-competitive inhibition. CNIModel approximated experimental data better than CIModel. © 2018 Polish Academy of Sciences. All Rights Reserved.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85056537976,

title = {Objectives, strategies and evaluation of the effectiveness of activated sludge bioaugmentation with defined microorganisms in the removal of toxic chemicals [Cele, Strategie i ocena efektywności bioaugmentacji Osadu czynnego zdefiniowanymi mikroorganizmami w usuwaniu toksycznych związków chemicznych]},

author = { J. Michalska and I. Greń and A. Mrozik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056537976&partnerID=40&md5=ea08ac5de29dd9fd6406aac6cd505a0a},

issn = {00794252},

year  = {2018},

date = {2018-01-01},

journal = {Postepy Mikrobiologii},

volume = {57},

number = {2},

pages = {125-137},

publisher = {Polish Society of Microbiologists},

abstract = {Among the currently used biological wastewater treatment systems, the conventional floccular-sludge method has been the most common. Its relatively novel alternative is aerobic granular activated sludge, which offers numerous operational and economic advantages. Although the activated sludge for modern wastewater treatment is often exposed to high concentrations of diverse chemicals, particularly inhibitory and recalcitrant ones, its autochthonous microorganisms may not be familiar with these compounds and can not use them as carbon and energy sources. For this reason, bioaugmentation, defined as a method for improvement of the degradative capacity of contaminated environment by adding selected strains or consortia of microorganisms, seems to be an attractive solution to overcome the problems associated with the exposure of sewage plants to high concentrations of xenobiotics. The most important step in the achievement of successful bioaugmentation is the selection of proper microorganisms with desirable abilities. They should be characterized by high degradative potential towards specific pollutant(s), ability to form biofilm, aggregation and production of extracellular polymeric substances, bioflocculating activity, motility, biosurfactants and autoinductors synthesis. Moreover, they should survive after inoculation into the activated sludge and possess the ability to incorporate into the flocs or form granules. In bioaugmentation of the activated sludge, several approaches can be distinguished - bioaugmentation with: single strains of bacteria or fungi, consortia of bacteria, consortia of fungi or mixed consortia, genetically modified microorganisms and commercial formulations. As many studies have indicated, bioaugmentation is an effective technology for eliminating from sewage toxic compounds, such as phenols and its derivatives, polycyclic aromatic hydrocarbons, dyes, pharmaceuticals and many others. © 2018 Polish Society of Microbiologists. All rights reserved.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84991728168,

title = {Changes in fatty acid composition of Stenotrophomonas maltophilia KB2 during co-metabolic degradation of monochlorophenols},

author = { A. Nowak and I. Greń and A. Mrozik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84991728168&doi=10.1007%2fs11274-016-2160-y&partnerID=40&md5=423eff60428251d50b8451cb15fb20c0},

doi = {10.1007/s11274-016-2160-y},

issn = {09593993},

year  = {2016},

date = {2016-01-01},

journal = {World Journal of Microbiology and Biotechnology},

volume = {32},

number = {12},

publisher = {Springer Netherlands},

abstract = {The changes in the cellular fatty acid composition of Stenotrophomonas maltophilia KB2 during co-metabolic degradation of monochlorophenols in the presence of phenol as well as its adaptive mechanisms to these compounds were studied. It was found that bacteria were capable of degrading 4-chlorophenol (4-CP) completely in the presence of phenol, while 2-chlorophenol (2-CP) and 3-chlorophenol (3-CP) they degraded partially. The analysis of the fatty acid profiles indicated that adaptive mechanisms of bacteria depended on earlier exposure to phenol, which isomer they degraded, and on incubation time. In bacteria unexposed to phenol the permeability and structure of their membranes could be modified through the increase of hydroxylated and cyclopropane fatty acids, and straight-chain and hydroxylated fatty acids under 2-CP, 3-CP and 4-CP exposure, respectively. In the exposed cells, regardless of the isomer they degraded, the most important changes were connected with the increase of the contribution of branched fatty acid on day 4 and the content of hydroxylated fatty acids on day 7. The changes, particularly in the proportion of branched fatty acids, could be a good indicator for assessing the progress of the degradation of monochlorophenols by S. maltophilia KB2. In comparison, in phenol-degrading cells the increase of cyclopropane and straight-chain fatty acid content was established. These findings indicated the degradative potential of the tested strain towards the co-metabolic degradation of persistent chlorophenols, and extended the current knowledge about the adaptive mechanisms of these bacteria to such chemicals. © 2016, The Author(s).},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84963854366,

title = {Microbial degradation of chlorophenols in soil [Mikrobiologiczny rozkład chlorofenoli w glebie]},

author = { A. Nowak and I. Greń and A. Mrozik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84963854366&partnerID=40&md5=f645ddbe6db7494bfc427571d4fe931e},

issn = {00794252},

year  = {2016},

date = {2016-01-01},

journal = {Postepy Mikrobiologii},

volume = {55},

number = {1},

pages = {79-90},

publisher = {Polish Society of Microbiologists},

abstract = {Chlorophenols are widely used in different branches of industry and agriculture as components of pesticides, disinfectants and wood treatment agents. They are hardly degradable and, therefore, can accumulate in the environment. One of the environment friendly methods of their removal from contaminated soil is microbial degradation. The efficiency of this process is determined by many abiotic and biotic factors. The first include the chemical structure of contaminants, their content and bioavailability as well as temperature, pH, soil texture, water content and oxygen concentration. In turn, biotic factors include the structure of microbial communities, stability and enzymatic activity of cells, their biomass, ability to chemotaxis and interactions between microorganisms. Several strategies have been developed to enhance the removal of chlorophenols from contaminated soil. The most effective in detoxifying these compounds are bioaugmentation, biostimulation and the use of "activated soil". In this review, the influence of different factors on microbial degradation of chlorophenols in soil are described and the applicability of selected methods in their removal are discussed.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84894638934,

title = {Enzymes involved in vinyl acetate decomposition by Pseudomonas fluorescens PCM 2123 strain},

author = { E. Szczyrba and I. Greń and G. Bartelmus},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894638934&doi=10.1007%2fs12223-013-0268-0&partnerID=40&md5=c92d2e843efaee479c7fabb77178fd29},

doi = {10.1007/s12223-013-0268-0},

issn = {00155632},

year  = {2014},

date = {2014-01-01},

journal = {Folia Microbiologica},

volume = {59},

number = {2},

pages = {99-105},

abstract = {Esterases are widely used in food processing industry, but there is little information concerning enzymes involved in decompositions of esters contributing to pollution of environment. Vinyl acetate (an ester of vinyl alcohol and acetic acid) is a representative of volatile organic compounds (VOCs) in decomposition, of which hydrolyses and oxidoreductases are mainly involved. Their activities under periodically changing conditions of environment are essential for the removal of dangerous VOCs. Esterase and alcohol/aldehyde dehydrogenase activities were determined in crude cell extract from Pseudomonas fluorescens PMC 2123 after vinyl acetate induction. All examined enzymes exhibit their highest activity at 30-35 °C and pH 7.0-7.5. Esterase preferably hydrolyzed ester bonds with short fatty chains without plain differences for C2 or C4. Comparison of Km values for alcohol and aldehyde dehydrogenases for acetaldehyde suggested that this metabolite was preferentially oxidized than reduced. Activity of alcohol dehydrogenase reducing acetaldehyde to ethanol suggested that one mechanism of defense against the elevated concentration of toxic acetaldehyde could be its temporary reduction to ethanol. Esterase activity was inhibited by phenylmethanesulfonyl fluoride, while β-mercaptoethanol, dithiothreitol, and ethylenediaminetetraacetic acid had no inhibitor effect. From among metal ions, only Mg2+ and Fe2+ stimulated the cleavage of ester bond. © 2013 The Author(s).},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84900524091,

title = {Microbial transformation of vanillic acid [Mikrobiologiczne przemiany kwasu wanilinowego]},

author = { M. Kurek and I. Greń},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84900524091&partnerID=40&md5=f2357928b65f8eb8933314ecbdb8c587},

issn = {00794252},

year  = {2014},

date = {2014-01-01},

journal = {Postepy Mikrobiologii},

volume = {53},

number = {1},

pages = {25-34},

publisher = {Polish Society of Microbiologists},

abstract = {Increasing demand for natural vanillic aroma in food industry as well as law restrictions for the usage of chemically synthesized compounds in natural fragrances caused large interest in vanillic production via biotechnological processes. Because vanillic acid is the main substrate for vanillic production, the knowledge of biological processes of its synthesis and the release from lignin is crucial for developing the optimal biotechnological processes. Some microorganisms are able to synthesize vanillic acid form naturally occurring compounds, such as ferulic acid, eugenol and isoeugenol using biotransformation reactions. Large amount of vanillin are produced by reduction of vanillic acid by carboxylic acid reductase (Car). Another pathways of vanillic acid transformation are based on its demethylation and decarboxylaction reactions. O-demethylases are NAH(P)H or tetrahydrofolic dependent enzymes. This review presents short characterization of vanillic acid transformation processes by microorganisms.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84874287322,

title = {Microbial transformation of xenobiotics},

author = { I. Greń},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874287322&partnerID=40&md5=f7dcdb34fda4f6675f6d287b82c0811d},

issn = {00092886},

year  = {2012},

date = {2012-01-01},

journal = {Chemik},

volume = {66},

number = {8},

pages = {839-842},

abstract = {As the result of human activity large amount of chemically different xenobiotics enter the environment every day. Physical and chemical properties of these compounds contribute to their accumulation in the environment, and their toxicity is dangerous for all living organisms. That's why microorganisms have been engaged for fight with xenobiotics through performing their biodegradation, mineralization or transformation. In this way microorganisms contribute to decrease the amount of xenobiotics in environment.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84856278951,

title = {Kinetics of styrene biodegradation by Pseudomonas sp. E-93486},

author = { A. Gąszczak and G. Bartelmus and I. Greń},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84856278951&doi=10.1007%2fs00253-011-3518-6&partnerID=40&md5=1be50994c0a5463ca3b922fbab4c74e7},

doi = {10.1007/s00253-011-3518-6},

issn = {01757598},

year  = {2012},

date = {2012-01-01},

journal = {Applied Microbiology and Biotechnology},

volume = {93},

number = {2},

pages = {565-573},

abstract = {The research into kinetics of styrene biodegradation by bacterial strain Pseudomonas sp. E-93486 coming from VTT Culture Collection (Finland) was presented in this work. Microbial growth tests in the presence of styrene as the sole carbon and energy source were performed both in batch and continuous cultures. Batch experiments were conducted for initial concentration of styrene in the liquid phase changed in the range of 5-90 gm -3. The Haldane model was found to be the best to fit the kinetic data, and the estimated constants of the equation were: μ m=0.1188 h -1, K S=5.984 mg l -1, and K i=156.6 mg l -1. The yield coefficient mean value for the batch culture was 0.72 g dry cells weight(g substrate) -1. The experiments conducted in a chemostat at various dilution rates (D=0.035-0.1 h -1) made it possible to determine the value of the coefficient for maintenance metabolism m d=0.0165 h -1 and the maximum yield coefficient value = 0.913. Chemostat experiments confirmed the high value of yield coefficient Y observed in the batch culture. The conducted experiments showed high activity of the examined strain in the styrene biodegradation process and a relatively low sensitivity to inhibition of its growth at higher concentrations of styrene in the solution. Such exceptional features of Pseudomonas sp. E-93486 make this bacterial strain the perfect candidate for technical applications. © 2011 The Author(s).},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79961208826,

title = {High activity catechol 2,3-dioxygenase from the cresols - Degrading Stenotrophomonas maltophilia strain KB2},

author = { D. Wojcieszyńska and K.T. Hupert-Kocurek and I. Greń and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79961208826&doi=10.1016%2fj.ibiod.2011.06.006&partnerID=40&md5=d6ef4d868b72531ce18499c1a567eb7c},

doi = {10.1016/j.ibiod.2011.06.006},

issn = {09648305},

year  = {2011},

date = {2011-01-01},

journal = {International Biodeterioration and Biodegradation},

volume = {65},

number = {6},

pages = {853-858},

abstract = {This study aimed at characterization of catechol 2,3-dioxygenase from Stenotrophomonas maltophilia KB2, being able to utilize a wide spectrum of aromatic substrates as a sole carbon and energy source. 2-methylphenol, 3-methylphenol, and 4-methylphenol was completely degraded during 24 h in concentration 6 mM, 7 mM, and 5 mM, respectively. When cells of strain KB2 were growing on methylphenols, catechol 2,3-dioxygenase was induced. Biochemical analysis revealed that the examined enzyme was similar to another catechol 2,3-dioxygenases, but showed extremely high activity. The enzyme was optimally active at 30 °C and pH 7.6. Kinetic studies showed that the value of Km, Vmax and Hill constant was 85.11 μM, 3.08 μM min-1 and 4.09 respectively. Comparative structural and phylogenetic analysis of catechol 2,3-dioxygenase from S. maltophilia KB2 had placed the protein with the single-ring substrate subfamily of the extradiol dioxygenase. We observed the presence of externally located α-helices and internally located β-sheets. We also suggest that the Fe2+ ion binding is facilitated via four ligands: two histidine residues, one glutamate residue and one molecule of water. © 2011 Elsevier Ltd.},

note = {31},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79960077339,

title = {A comparative study of biodegradation of vinyl acetate by environmental strains},

author = { I. Greń and A. Gąszczak and U. Guzik and G. Bartelmus and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960077339&doi=10.1007%2fs13213-010-0130-4&partnerID=40&md5=dc8b236cbaf2ce1152f3d98ece583042},

doi = {10.1007/s13213-010-0130-4},

issn = {15904261},

year  = {2011},

date = {2011-01-01},

journal = {Annals of Microbiology},

volume = {61},

number = {2},

pages = {257-265},

abstract = {Four Gram-negative strains, E3-2001, EC1-2004, EC3-3502 and EC2-3502, previously isolated from soil samples, were subjected to comparative studies in order to select the best vinyl acetate degrader for waste gas treatment. Comparison of biochemical and physiological tests as well as the results of fatty acids analyses were comparable with the results of 16S rRNA gene sequence analyses. The isolated strains were identified as Pseudomonas putida EC3-2001, Pseudomonas putida EC1-2004, Achromobacter xylosoxidans EC3-3502 and Agrobacterium sp. EC2-3502 strains. Two a dd it io na l str ai ns, Pseudomonas fluorescens PCM 2123 and Stenotrophomonas malthophilia KB2, were used as controls. All described strains were able to use vinyl acetate as the only source of carbon and energy under aerobic as well as oxygen deficiency conditions. Esterase, alcohol dehydrogenase and aldehyde dehydrogenase were involved in vinyl acetate decomposition under aerobic conditions. Shorter degradation times of vinyl acetate were associated with accumulation of acetic acid, acetaldehyde and ethanol as intermediates in the culture fluids of EC3-2001 and KB2 strains. Complete aerobic degradation of vinyl acetate combined with a low increase in biomass was observed for EC3-2001 and EC1-2004 strains. In conclusion, P. putida EC1-2004 is proposed as the best vinyl acetate degrader for future waste gas treatment in trickle-bed bioreactors. © The Author(s) 2010..},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79953107665,

title = {Catechol 1,2-dioxygenase from the new aromatic compounds - Degrading Pseudomonas putida strain N6},

author = { U. Guzik and I. Greń and K.T. Hupert-Kocurek and D. Wojcieszyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79953107665&doi=10.1016%2fj.ibiod.2011.02.001&partnerID=40&md5=7fa3eb471f0eb2071b4a850b40265e4b},

doi = {10.1016/j.ibiod.2011.02.001},

issn = {09648305},

year  = {2011},

date = {2011-01-01},

journal = {International Biodeterioration and Biodegradation},

volume = {65},

number = {3},

pages = {504-512},

abstract = {This study aimed to characterization of catechol 1,2-dioxygenase from a Gram-negative bacterium, being able to utilize a wide spectrum of aromatic substrates as a sole carbon and energy source. Strain designated as N6, was isolated from the activated sludge samples of a sewage treatment plant at Bentwood Furniture Factory Jasienica, Poland. Morphology, physio-biochemical characteristics and phylogenetic analysis based on 16S rDNA sequence indicate that strain belongs to Pseudomonas putida. When cells of strain N6 grown on protocatechuate or 4-hydroxybenzoic acid mainly protocatechuate 3,4-dioxygenase was induced. The activity of catechol 1,2-dioxygenase was rather small. The cells grown on benzoic acid, catechol or phenol showed high activity of only catechol 1,2-dioxygenase. This enzyme was optimally active at 35°C and pH 7.4. Kinetic studies showed that the value of Km and Vmax was 85.19μM and 14.54μMmin-1 respectively. Nucleotide sequence of gene encoding catechol 1,2-dioxygenase in strain N6 has 100% identity with catA genes from two P. putida strains. The deduced 301-residue sequence of enzyme corresponds to a protein of molecular mass 33.1kDa. The deduced molecular structure of the catechol 1,2-dioxygenase from P. putida N6 was very similar and characteristic for the other intradiol dioxygenases. © 2011 Elsevier Ltd.},

note = {45},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79952572933,

title = {Induction of aromatic ring: Cleavage dioxygenases in Stenotrophomonas maltophilia strain KB2 in cometabolic systems},

author = { D. Wojcieszyńska and U. Guzik and I. Greń and M. Perkosz and K.T. Hupert-Kocurek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952572933&doi=10.1007%2fs11274-010-0520-6&partnerID=40&md5=c1266ef2c54e06fa02156c466ae15eed},

doi = {10.1007/s11274-010-0520-6},

issn = {09593993},

year  = {2011},

date = {2011-01-01},

journal = {World Journal of Microbiology and Biotechnology},

volume = {27},

number = {4},

pages = {805-811},

abstract = {Stenotrophomonas maltophilia KB2 is known to produce different enzymes of dioxygenase family. The aim of our studies was to determine activity of these enzymes after induction by benzoic acids in cometabolic systems with nitrophenols. We have shown that under cometabolic conditions KB2 strain degraded 0.25-0.4 mM of nitrophenols after 14 days of incubation. Simultaneously degradation of 3 mM of growth substrate during 1-3 days was observed depending on substrate as well as cometabolite used. From cometabolic systems with nitrophenols as cometabolites and 3,4-dihydroxybenzoate as a growth substrate, dioxygenases with the highest activity of protocatechuate 3,4-dioxygenase were isolated. Activity of catechol 1,2- dioxygenase and protocatechuate 4,5-dioxygenase was not observed. Catechol 2,3-dioxygenase was active only in cultures with 4-nitrophenol. Ability of KB2 strain to induce and synthesize various dioxygenases depending on substrate present in medium makes this strain useful in bioremediation of sites contaminated with different aromatic compounds. © 2010 The Author(s).},

note = {48},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-81355139502,

title = {Modulation of FAD-dependent monooxygenase activity from aromatic compounds-degrading Stenotrophomonas maltophilia strain KB2},

author = { D. Wojcieszyńska and I. Greń and K.T. Hupert-Kocurek and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-81355139502&doi=10.18388%2fabp.2011_2256&partnerID=40&md5=cf851e7c3fa3a0bbc0603a8971168364},

doi = {10.18388/abp.2011_2256},

issn = {0001527X},

year  = {2011},

date = {2011-01-01},

journal = {Acta Biochimica Polonica},

volume = {58},

number = {3},

pages = {421-426},

publisher = {Acta Biochimica Polonica},

abstract = {The purpose of this study was purification and characterization of phenol monooxygenase from Stenotrophomonas maltophilia strain KB2, enzyme that catabolises phenol and its derivatives through the initial hydroxylation to catechols. The enzyme requires NADH and FAD as a cofactors for activity, catalyses hydroxylation of a wide range of monocyclic phenols, aromatic acids and dihydroxylated derivatives of benzene except for catechol. High activity of this monooxygenase was observed in cell extract of strain KB2 grown on phenol, 2-methylphenol, 3-metylphenol or 4-methylphenol. Ionic surfactants as well as cytochrome P450 inhibitors or 1,4-dioxane, acetone and n-butyl acetate inhibited the enzyme activity, while non-ionic surfactants, chloroethane, ethylbenzene, ethyl acetate, cyclohexane, and benzene enhanced it. These results indicate that the phenol monooxygenase from Stenotrophomonas maltophilia strain KB2 holds great potential for bioremediation.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84862875187,

title = {Activity of catechol dioxygenases in the presence of some heavy metal ions: Bioremediation of an environment polluted with aromatic compounds [Badania aktywności dioksygenaz katecholowych w obecności jonów wybranych metali ciȩżkich w aspekcie bioremediacji środowisk zanieczyszczonych zwia̧zkami aromatycznymi]},

author = { U. Guzik and D. Wojcieszyńska and I. Greń and K.T. Hupert-Kocurek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862875187&partnerID=40&md5=86313da0b90e3633bb0a7ddecc001794},

issn = {12306169},

year  = {2010},

date = {2010-01-01},

journal = {Ochrona Srodowiska},

volume = {32},

number = {1},

pages = {9-13},

abstract = {The strains of Stenotrophomonas maltophilia KB2 and Pseudomonas putida N6 are characterized by an enhanced capacity for degrading aromatic compounds: within five hours of incubation both the strains were found to provide a complete degradation of phenol (3 mmol/dm3). Upon induction with phenol, catechol 2,3-dioxygenase, an enzyme responsible for the meta-cleavage of aromatic compounds, was detected in the Stenotrophomonas maltophilia KB2 strain, whereas in the Pseudomonas putida N6 strain the presence was revealed of catechol 1,2-dioxygenase, an enzyme characteristic of the pathway for the ortho-fission of the aromatic ring. Tests on the sensitivity of the enzymes to metal ions have demonstrated that Zn 2+ ions activated catechol 2,3-dioxygenase in the KB2 strain. The other metal ions were found to be inhibitors of this enzyme. Among the metal ions tested, the Cu 2+ ion was the strongest inhibitor of the two isolated dioxynases. Slightly weaker was the inhibition of catechol 1,2-dioxygenase induced by Cd 2+ and Zn 2+ ions in the N6 strain. The activity of this enzyme increased in the presence of Co 2+ ions. The other ions had no significant influence on the activity of the catechol 1,2-dioxygenase isolated from the N6 strain. The partial activity of both dioxygenases observed upon the application of metal salts suggests that both the strains, Stenotrophomonas maltophilia KB2 and Pseudomonas putida N6, may contribute much to the remediation of an environment polluted with aromatic compounds.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77951141722,

title = {Enhanced biotransformation of mononitrophenols by Stenotrophomonas maltophilia KB2 in the presence of aromatic compounds of plant origin},

author = { I. Greń and D. Wojcieszyńska and U. Guzik and M. Perkosz and K.T. Hupert-Kocurek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951141722&doi=10.1007%2fs11274-009-0172-6&partnerID=40&md5=10c990c0b3a65918797b592059052a18},

doi = {10.1007/s11274-009-0172-6},

issn = {09593993},

year  = {2010},

date = {2010-01-01},

journal = {World Journal of Microbiology and Biotechnology},

volume = {26},

number = {2},

pages = {289-295},

abstract = {Stenotrophomonas maltophilia KB2 used in this study is known to metabolise broad range of aromatic compounds including phenol, some chloro and methylphenols, benzoic acids, catochols and others. To study the applicability of the strain for degradation of mononitrophenols in monosubstrate as well as cometabolic systems its degradation potential in the presence of mononitrophenols or different aromatic compounds of plant origin was tested. Stenotrophomonas maltophilia KB2 strain was not able to degrade any of mononitrophenols used in the single substrate experiments. Effect of additional carbon source on nitrophenols degradation revealed that presence of benzoate, 4-hydroxybenzoate or 3,4-dixydroxybenzoate stimulate transformation of 2-nitrophenol, 3-nitrophenol as well as 4-nitrophenol. Depending on growth substrate and mononitrophenol used, decrease in cometabolite concentration was from 25 to 45%. Obtained results suggest that Stenotrophomonas maltophilia KB2 strain could be potentially used for cometabolic degradation of nitrophenols in the presence of aromatic acids, for the bioremediation of contaminated sites. © 2009 Springer Science+Business Media B.V.},

note = {49},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-67650497833,

title = {Enrichment, isolation and susceptibility profile of the growth substrate of bacterial strains able to degrade vinyl acetate},

author = { I. Greń and A. Gąszczak and E. Szczyrba and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-67650497833&partnerID=40&md5=e0dd68fa0f310f7d734fd346ad548612},

issn = {12301485},

year  = {2009},

date = {2009-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {18},

number = {3},

pages = {383-390},

abstract = {Nowadays biodegradations of harmful xenobiotics seems to be the best and cheapest method of purification of the polluted environment. VOCs are represented by vinyl acetate, which is thought to be carcinogenic. The aim of these studies was to isolate and determine the susceptibility profile for vinyl acetate of bacterial strains. The source of microorganisms was soil sampled in the area of Synthos S.A. in Oświȩcim, Poland. From among 41 isolates, 4 Gram-negative strains were chosen for further analyses. As the control, one laboratory strain of Pseudomonas Jluorescens PCM 2123 from The Polish Collection of Microorganisms (Wroclaw) was used. Simultaneously, a susceptibility profile to vinyl acetate was performed on Stenotrophomonas maltophilia KB2 strain, aromatic compounds' degrader. Vinyl acetate used in concentration of 3,000 ppm inhibited growth of gram-positive bacteria, and 4,000 ppm was the lethal dose for microorganisms from mixed populations. A toxicity test showed susceptibility to vinyl acetate at concentrations of 2,000 ppm. Three weeks of pre-incubation with 400 ppm of vinyl acetate magnified the level of sensitivity to 3,000 ppm of vinyl acetate for almost all strains. Although decomposition of vinyl acetate was observed even in the presence of4,000,5,000 and 6,000 ppm of vinyl acetate, growth was not observed. It was due to enlarged concentration of acetaldehyde, a product of hydrolysis ester bond of vinyl acetate.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-51149097365,

title = {Dioxygenases - The key enzymes in the biodegradation of aromatic compounds [Dioksygenazy - Główne enzymy degradacji zwia̧zkó w aromatycznych]},

author = { U. Guzik and I. Greń and D. Wojcieszyńska and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-51149097365&partnerID=40&md5=f18a5821b0637c819e7d792486cd3579},

issn = {08607796},

year  = {2008},

date = {2008-01-01},

journal = {Biotechnologia},

number = {3},

pages = {71-88},

publisher = {Termedia Publishing House Ltd.},

abstract = {Progressing degradation of the natural environment has caused an increased interest in the field of biological treatment of both water and soil polluted with different xenobiotics. Aromatic compounds are considered to be one of the most toxic and weakly degraded xenobiotics. The potential solution of the accelerated environment's pollution problem seems to be the bioremediation - the process using the biological organisms to return the environment altered by contaminants to its original condition. Dioxygenases isolated from the microorganisms can be responsible for hydroxylation of the aromatic ring or for its cleavage. Different types of the cleaving dioxygenases have been distinguished due to the kind of the substrate they preferentially can degrade. Cleavage of the aromatic ring facilitates further degradation of xenobiotics, and therefore the dioxygenases are the key enzymes in the biodegradation process of these xenobiotics.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34547240731,

title = {Substrate specificity and sensitiveness of phenol monooxygenase from Stenotropbomonas maltopbilia strain KB2 versus their potential application to bioremediation of the environment [Specyficzność substratowa oraz wrażiwość monooksygenazy fenolowej ze szczepu Stenotrophomonas maltophilia KB2 a jej potencjalne zastosowanie w bioremediacji środowiska]},

author = { D. Wojcieszyńska and I. Greń and S. Łabuzek and M. Respondek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547240731&partnerID=40&md5=4fc32759232a602f03b9d98cba5d9906},

issn = {08607796},

year  = {2007},

date = {2007-01-01},

journal = {Biotechnologia},

number = {2},

pages = {181-191},

publisher = {Termedia Publishing House Ltd.},

abstract = {Phenol monooxygenase, isolated from Stenotrophomonas maltophilia strain KB2, was sensitive to sodium azide, metals salts except for iron (II) sulfate at concentration of 1 mM, chelate compounds, sulfhydryl agents, lauroylsarcosine Na-salt, SDS and hydrogen peroxide. Slight increase of the enzyme activity was observed in the presence of hexane and ether. The presence of ascorbic acid caused an increase of the enzyme activity. Phenol monooxygenase activity changed significantly depending on the tested aromatic substrate in the reaction mixture and the type of the applied inductor.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-24744457078,

title = {Dioxygenases-key enzymes for degradation of aromatic compounds by microorganisms [Dioksygenazy-kluczowe enzymy rozkŁadu zwia̧zków aromatycznych przez drobnoustroje]},

author = { D. Wojcieszyńska and I. Greń and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-24744457078&partnerID=40&md5=a4941e3b5b29d9e25531b0f3aad4cc19},

issn = {00794252},

year  = {2005},

date = {2005-01-01},

journal = {Postepy Mikrobiologii},

volume = {44},

number = {1},

pages = {63-70},

abstract = {Oxygen-activating enzymes with mononuclear non-heme active participate in many important metabolic pathways of the environmental significance. The ring dihydroxylation is non-specific preliminary step in the catabolic pathway and plays an important role in the activation of resonance-stabilized aromatic compounds before the subsequent catabolism. Dihydroxylate intermediates such as catechol, hydroxyquinol or protocatechuate are cleavaged between their two hydroxyl groups (ortho cleavage) by catechol 1,2-dioxygenase, hydroxyquinol 1,2-dioxygenase or protocatechuate 3,4-dioxygenase. The iron atom in the pentacoordinate active centre of these enzymes remains in the high-spin Fe (III) state during catalysis. Extradiol dioxygenases catalyze the ring-cleavage at the C-C bond adjacent to the vicinal hydroxyl groups. These dioxygenases typically contain non-heme iron Fe (II) in their active site but have been also shown to be active with Mn (II). The catechol 2,3-dioxygenase catalytic cycle is supposed to comprise a complexion of iron ion by monoanionic catecholate as a bidentate ligand. The ring cleavage reaction is proposed to proceed via an attack of the iron-bound activated oxygen on the nonhydroxylated position vicinal to the carbon atom bearing the phenolate anion. When extradiol dioxygenases cleave 3-chlorocatechol, they usually become inactivated. This inactivation might be caused by the strong chelating activity of 3-chlorocatechol or by suicide inactivation of the enzyme due to the formation of reactive intermediate.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}