@article{2-s2.0-85115044224,

title = {Updates on the role of ABSCISIC ACID INSENSITIVE 5 (ABI5) and ABSCISIC ACID-RESPONSIVE ELEMENT BINDING FACTORS (ABFs) in ABA signaling in different developmental stages in plants},

author = { A. Collin and A. Daszkowska-Golec and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115044224&doi=10.3390%2fcells10081996&partnerID=40&md5=288aae4e7889cefdab714eb59b407ca5},

doi = {10.3390/cells10081996},

issn = {20734409},

year  = {2021},

date = {2021-01-01},

journal = {Cells},

volume = {10},

number = {8},

publisher = {MDPI},

abstract = {The core abscisic acid (ABA) signaling pathway consists of receptors, phosphatases, ki-nases and transcription factors, among them ABA INSENSITIVE 5 (ABI5) and ABRE BINDING FACTORs/ABRE-BINDING PROTEINs (ABFs/AREBs), which belong to the BASIC LEUCINE ZIPPER (bZIP) family and control expression of stress-responsive genes. ABI5 is mostly active in seeds and prevents germination and post-germinative growth under unfavorable conditions. The activity of ABI5 is controlled at transcriptional and protein levels, depending on numerous regulators, including components of other phytohormonal pathways. ABFs/AREBs act redundantly in regulating genes that control physiological processes in response to stress during vegetative growth. In this review, we focus on recent reports regarding ABI5 and ABFs/AREBs functions during abiotic stress responses, which seem to be partially overlapping and not restricted to one developmental stage in Arabidopsis and other species. Moreover, we point out that ABI5 and ABFs/AREBs play a crucial role in the core ABA pathway’s feedback regulation. In this review, we also discuss increased stress tolerance of transgenic plants overexpressing genes encoding ABA-dependent bZIPs. Taken to-gether, we show that ABI5 and ABFs/AREBs are crucial ABA-dependent transcription factors regulating processes essential for plant adaptation to stress at different developmental stages. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85088304268,

title = {Barley strigolactone signalling mutant hvd14.d reveals the role of strigolactones in abscisic acid-dependent response to drought},

author = { M. Marzec and A. Daszkowska-Golec and A. Collin and M. Melzer and K. Eggert and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088304268&doi=10.1111%2fpce.13815&partnerID=40&md5=a17d98cfe41937b5140917b7f9836499},

doi = {10.1111/pce.13815},

issn = {01407791},

year  = {2020},

date = {2020-01-01},

journal = {Plant Cell and Environment},

volume = {43},

number = {9},

pages = {2239-2253},

publisher = {Blackwell Publishing Ltd},

abstract = {Strigolactones (SLs) are a group of plant hormones involved in many aspects of plant development and stress adaptation. Here, we investigated the drought response of a barley (Hordeum vulgare L.) mutant carrying a missense mutation in the gene encoding the SL-specific receptor HvD14. Our results clearly showed that hvd14.d mutant is hyper-sensitive to drought stress. This was illustrated by a lower leaf relative water content (RWC), impaired photosynthesis, disorganization of chloroplast structure, altered stomatal density and slower closure of stomata in response to drought in the mutant compared to the wild type parent cultivar Sebastian. Although the content of abscisic acid (ABA) and its derivatives remained unchanged in the mutant, significant differences in expression of genes related to ABA biosynthesis were observed. Moreover, hvd14.d was insensitive to ABA during seed germination. Analysis of Arabidopsis thaliana mutant atd14-1 also demonstrated that mutation in the SL receptor resulted in increased sensitivity to drought. Our results indicate that the drought-sensitive phenotype of barley SL mutant might be caused by a disturbed ABA metabolism and/or signalling pathways. These results together uncovered a link between SL signalling and ABA-dependent drought stress response in barley. © 2020 John Wiley & Sons Ltd.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85089435133,

title = {Barley ABI5 (Abscisic Acid INSENSITIVE 5) Is Involved in Abscisic Acid-Dependent Drought Response},

author = { A. Collin and A. Daszkowska-Golec and M.M. Kurowska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089435133&doi=10.3389%2ffpls.2020.01138&partnerID=40&md5=ab2494c5d3f9cd41dd76be0da41911a1},

doi = {10.3389/fpls.2020.01138},

issn = {1664462X},

year  = {2020},

date = {2020-01-01},

journal = {Frontiers in Plant Science},

volume = {11},

publisher = {Frontiers Media S.A.},

abstract = {ABA INSENSITIVE 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor which acts in the abscisic acid (ABA) network and is activated in response to abiotic stresses. However, the precise role of barley (Hordeum vulgare) ABI5 in ABA signaling and its function under stress remains elusive. Here, we show that HvABI5 is involved in ABA-dependent regulation of barley response to drought stress. We identified barley TILLING mutants carrying different alleles in the HvABI5 gene and we studied in detail the physiological and molecular response to drought and ABA for one of them. The hvabi5.d mutant, carrying G1751A transition, was insensitive to ABA during seed germination, yet it showed the ability to store more water than its parent cv. “Sebastian” (WT) in response to drought stress. The drought-tolerant phenotype of hvabi5.d was associated with better membrane protection, higher flavonoid content, and faster stomatal closure in the mutant under stress compared to the WT. The microarray transcriptome analysis revealed up-regulation of genes associated with cell protection mechanisms in the mutant. Furthermore, HvABI5 target genes: HVA1 and HVA22 showed higher activity after drought, which may imply better adaptation of hvabi5.d to stress. On the other hand, chlorophyll content in hvabi5.d was lower than in WT, which was associated with decreased photosynthesis efficiency observed in the mutant after drought treatment. To verify that HvABI5 acts in the ABA-dependent manner we analyzed expression of selected genes related to ABA pathway in hvabi5.d and its WT parent after drought and ABA treatments. The expression of key genes involved in ABA metabolism and signaling differed in the mutant and the WT under stress. Drought-induced increase of expression of HvNCED1, HvBG8, HvSnRK2.1, and HvPP2C4 genes was 2–20 times higher in hvabi5.d compared to “Sebastian”. We also observed a faster stomatal closure in hvabi5.d and much higher induction of HvNCED1 and HvSnRK2.1 genes after ABA treatment. Together, these findings demonstrate that HvABI5 plays a role in regulation of drought response in barley and suggest that HvABI5 might be engaged in the fine tuning of ABA signaling by a feedback regulation between biosynthetic and signaling events. In addition, they point to different mechanisms of HvABI5 action in regulating drought response and seed germination in barley. © Copyright © 2020 Collin, Daszkowska-Golec, Kurowska and Szarejko.},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85076778671,

title = {Genetic and physiological dissection of photosynthesis in barley exposed to drought stress},

author = { A. Daszkowska-Golec and A. Collin and K. Sitko and A. Janiak and H.M. Kalaji and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076778671&doi=10.3390%2fijms20246341&partnerID=40&md5=14faebbe60c567c85947758707f74195},

doi = {10.3390/ijms20246341},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {24},

publisher = {MDPI AG},

abstract = {Balanced photosynthesis under drought is essential for better survival and for agricultural benefits in terms of biomass and yield. Given the current attempts to improve the photosynthetic efficiency for greater crop yield, the explanation of the genetic basis of that process, together with the phenotypic analysis, is significant in terms of both basic studies and potential agricultural application. Therefore, the main objective of this study was to uncover the molecular basis of the photosynthesis process under drought stress in barley. To address that goal, we conducted transcriptomic examination together with detailed photosynthesis analysis using the JIP-test. Using this approach, we indicated that photosynthesis is a process that is very early affected in barley seedlings treated with severe drought stress. Rather than focusing on individual genes, our strategy was pointed to the identification of groups of genes with similar expression patterns. As such, we identified and annotated almost 150 barley genes as crucial core-components of photosystems, electron transport components, and Calvin cycle enzymes. Moreover, we designated 17 possible regulatory interactions between photosynthesis-related genes and transcription factors in barley. Summarizing, our results provide a list of candidate genes for future genetic research and improvement of barley drought tolerance by targeting photosynthesis. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85040322145,

title = {Mutation in barley ERA1 (Enhanced Response to ABA1) gene confers better photosynthesis efficiency in response to drought as revealed by transcriptomic and physiological analysis},

author = { A. Daszkowska-Golec and A. Collin and K. Sitko and M. Słota and M.M. Kurowska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040322145&doi=10.1016%2fj.envexpbot.2018.01.003&partnerID=40&md5=0007d532bbcbed5056c705585b2fd044},

doi = {10.1016/j.envexpbot.2018.01.003},

issn = {00988472},

year  = {2018},

date = {2018-01-01},

journal = {Environmental and Experimental Botany},

volume = {148},

pages = {12-26},

publisher = {Elsevier B.V.},

abstract = {Farnesylation is a post-translational modification that promotes the interaction between the modified signaling protein and membrane lipids and/or other proteins. Farnesyltransferase is the crucial enzyme involved in this process. Strikingly, plant mutants in the ERA1 (Enhanced response to ABA 1) gene, encoding β-subunit of farnesyltransferase, exhibited ABA-hypersensitivity during seed germination and drought tolerance in several species including Arabidopsis, wheat and soybean. However, the mechanism of ERA1 action has not been resolved yet. Here, we present the potential regulatory role of ERA1 in the drought signaling network in barley. With the aim of decoding the role of the ERA1 gene, we developed a unique barley mutant using TILLING analysis. Mutation in HvERA1 confers semi-dwarf phenotype, ABA-sensitivity during seed germination and drought tolerance. Our transcriptomic analysis suggested a role of HvERA1 in regulation of the crosstalk between ABA and ethylene at the onset of drought. Furthermore, analysis of hvera1.b response to prolonged drought stress linked HvERA1 to the metabolism of galactolipids, that build the chloroplast membranes. It might results in the protection of hvera1.b photosystem and thus, in its better photosynthesis performance under water stress. Together, these results indicate the possible mechanism of the primary cause of the observed alterations in the hvera1.b mutant. © 2018 Elsevier B.V.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021152716,

title = {Mutation in HvCBP20 (Cap binding protein 20) adapts barley to drought stress at phenotypic and transcriptomic levels},

author = { A. Daszkowska-Golec and A. Collin and M. Marzec and M. Słota and M.M. Kurowska and M. Gajecka and P. Gajewska and T. Płociniczak and K. Sitko and A. Pacak and Z. Szweykowska-Kulinska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021152716&doi=10.3389%2ffpls.2017.00942&partnerID=40&md5=769fb24d1f52b6fc895f399773d7ea69},

doi = {10.3389/fpls.2017.00942},

issn = {1664462X},

year  = {2017},

date = {2017-01-01},

journal = {Frontiers in Plant Science},

volume = {8},

publisher = {Frontiers Media S.A.},

abstract = {CBP20 (Cap-Binding Protein 20) encodes a small subunit of the cap-binding complex (CBC), which is involved in the conserved cell processes related to RNA metabolism in plants and, simultaneously, engaged in the signaling network of drought response, which is dependent on ABA. Here, we report the enhanced tolerance to drought stress of barley mutant in the HvCBP20 gene manifested at the morphological, physiological, and transcriptomic levels. Physiological analyses revealed differences between the hvcbp20.ab mutant and its WT in response to a water deficiency. The mutant exhibited a higher relative water content (RWC), a lower stomatal conductance and changed epidermal pattern compared to the WT after drought stress. Transcriptome analysis using the Agilent Barley Microarray integrated with observed phenotypic traits allowed to conclude that the hvcbp20.ab mutant exhibited better fitness to stress conditions by its much more efficient and earlier activation of stress-preventing mechanisms. The network hubs involved in the adjustment of hvcbp20.ab mutant to the drought conditions were proposed. These results enabled to make a significant progress in understanding the role of CBP20 in the drought stress response. © 2017 Daszkowska-Golec, Skubacz, Marzec, Slota, Kurowska, Gajecka, Gajewska, Płociniczak, Sitko, Pacak, Szweykowska-Kulinska and Szarejko.},

note = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85007338538,

title = {The role and regulation of ABI5 (ABA-insensitive 5) in plant development, abiotic stress responses and phytohormone crosstalk},

author = { A. Collin and A. Daszkowska-Golec and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85007338538&doi=10.3389%2ffpls.2016.01884&partnerID=40&md5=a947006f38a2703ae6a7bbfb624d4ed2},

doi = {10.3389/fpls.2016.01884},

issn = {1664462X},

year  = {2016},

date = {2016-01-01},

journal = {Frontiers in Plant Science},

volume = {7},

number = {DECEMBER2016},

publisher = {Frontiers Media S.A.},

abstract = {ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth in the presence of ABA and abiotic stresses. ABI5 functions in the core ABA signaling, which is composed of PYR/PYL/RCAR receptors, PP2C phosphatases and SnRK2 kinases, through the regulation of the expression of genes that contain the ABSCISIC ACID RESPONSE ELEMENT (ABRE) motif within their promoter region. The regulated targets include stress adaptation genes, e.g., LEA proteins. However, the expression and activation of ABI5 is not only dependent on the core ABA signaling. Many transcription factors such as ABI3, ABI4, MYB7 and WRKYs play either a positive or a negative role in the regulation of ABI5 expression. Additionally, the stability and activity of ABI5 are also regulated by other proteins through post-translational modifications such as phosphorylation, ubiquitination, sumoylation and S-nitrosylation. Moreover, ABI5 also acts as an ABA and other phytohormone signaling integrator. Components of auxin, cytokinin, gibberellic acid, jasmonate and brassinosteroid signaling and metabolism pathways were shown to take part in ABI5 regulation and/or to be regulated by ABI5. Monocot orthologs of AtABI5 have been identified. Although their roles in the molecular and physiological adaptations during abiotic stress have been elucidated, knowledge about their detailed action still remains elusive. Here, we describe the recent advances in understanding the action of ABI5 in early developmental processes and the adaptation of plants to unfavorable environmental conditions. We also focus on ABI5 relation to other phytohormones in the abiotic stress response of plants. © 2016 Skubacz, Daszkowska-Golec and Szarejko.},

note = {216},

keywords = {},

pubstate = {published},

tppubtype = {article}

}