• dr Ewa Sybilska
Stanowisko: Biolog
Jednostka: Biuro ds. Infrastruktury Badawczo-Dydaktycznej WNP
Adres: 40-032 Katowice, ul. Jagiellońska 28
Piętro: II
Numer pokoju: C-248
Telefon: (32) 2009 457
E-mail: ewa.sybilska@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 58174552200
Publikacje z bazy Scopus
2025
Matkowski, H.; Collin, A.; Sybilska, E.; Potocka, I. W.; Daszkowska-Golec, A.
Barley nuclear cap-binding complex subunits, HvCBP20 and HvCBP80, play distinct roles in drought adaptation at reproductive phase of development Journal Article
In: Environmental and Experimental Botany, vol. 240, 2025, ISSN: 00988472, (0).
@article{2-s2.0-105021122861,
title = {Barley nuclear cap-binding complex subunits, HvCBP20 and HvCBP80, play distinct roles in drought adaptation at reproductive phase of development},
author = { H. Matkowski and A. Collin and E. Sybilska and I.W. Potocka and A. Daszkowska-Golec},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-105021122861&doi=10.1016%2Fj.envexpbot.2025.106266&partnerID=40&md5=ea7e9e7c46633d1bd17305c6faecb83d},
doi = {10.1016/j.envexpbot.2025.106266},
issn = {00988472},
year = {2025},
date = {2025-01-01},
journal = {Environmental and Experimental Botany},
volume = {240},
publisher = {Elsevier B.V.},
abstract = {Drought remains a major constraint to crop productivity. The nuclear cap-binding complex (CBC), composed of CBP20 and CBP80, regulates pre-mRNA splicing and has been increasingly associated with abscisic acid (ABA) signaling, as suggested by recent studies. Here, we investigated the transcriptomic and physiological impacts of mutations in genes encoding barley nuclear CBC ( hvcbp20.ab; hvcbp80 . b ; and hvcbp20.ab/hvcbp80.b ) under drought applied at the booting stage. The mutants exhibited both shared- and mutation-specific adaptations to drought. Transcriptomic profiling revealed that mutation in HvCBP80 significantly reduced transcriptional and splicing activities while inducing the expression of photosynthesis-related genes, resulting in enhanced photosynthetic efficiency under both optimal and drought conditions. Conversely, mutation in HvCBP20 intensified ABA-responsive gene expression and prolonged stress signaling. Physiologically, the hvcbp20.ab mutants displayed increased stomatal conductance despite reduced stomatal density, whereas the hvcbp80.b mutants exhibited decreased conductance under optimal conditions. Despite improved photosynthesis and dehydration avoidance traits, none of the mutations enhanced yield-related parameters under either optimal or drought conditions. Our findings establish that nuclear CBC is a pivotal regulator of drought stress responses and rewatering, capable of reprogramming the transcriptomic landscape to promote enhanced barley resilience. © 2025 The Authors.},
note = {0},
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pubstate = {published},
tppubtype = {article}
}
Drought remains a major constraint to crop productivity. The nuclear cap-binding complex (CBC), composed of CBP20 and CBP80, regulates pre-mRNA splicing and has been increasingly associated with abscisic acid (ABA) signaling, as suggested by recent studies. Here, we investigated the transcriptomic and physiological impacts of mutations in genes encoding barley nuclear CBC ( hvcbp20.ab; hvcbp80 . b ; and hvcbp20.ab/hvcbp80.b ) under drought applied at the booting stage. The mutants exhibited both shared- and mutation-specific adaptations to drought. Transcriptomic profiling revealed that mutation in HvCBP80 significantly reduced transcriptional and splicing activities while inducing the expression of photosynthesis-related genes, resulting in enhanced photosynthetic efficiency under both optimal and drought conditions. Conversely, mutation in HvCBP20 intensified ABA-responsive gene expression and prolonged stress signaling. Physiologically, the hvcbp20.ab mutants displayed increased stomatal conductance despite reduced stomatal density, whereas the hvcbp80.b mutants exhibited decreased conductance under optimal conditions. Despite improved photosynthesis and dehydration avoidance traits, none of the mutations enhanced yield-related parameters under either optimal or drought conditions. Our findings establish that nuclear CBC is a pivotal regulator of drought stress responses and rewatering, capable of reprogramming the transcriptomic landscape to promote enhanced barley resilience. © 2025 The Authors.
Sybilska, E.; Haddadi, B. S.; Mur, L. A. J.; Beckmann, M.; Hryhorowicz, S. T.; Suszyńska-Zajczyk, J.; Knaur, M.; Plawski, A.; Daszkowska-Golec, A.
Mapping the molecular signature of ABA-regulated gene expression in germinating barley embryos Journal Article
In: BMC Plant Biology, vol. 25, no. 1, 2025, ISSN: 14712229, (3).
@article{2-s2.0-105004698412,
title = {Mapping the molecular signature of ABA-regulated gene expression in germinating barley embryos},
author = { E. Sybilska and B.S. Haddadi and L.A.J. Mur and M. Beckmann and S.T. Hryhorowicz and J. Suszyńska-Zajczyk and M. Knaur and A. Plawski and A. Daszkowska-Golec},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-105004698412&doi=10.1186%2Fs12870-025-06654-z&partnerID=40&md5=1114d84e253745f596e2560ebd1b67a9},
doi = {10.1186/s12870-025-06654-z},
issn = {14712229},
year = {2025},
date = {2025-01-01},
journal = {BMC Plant Biology},
volume = {25},
number = {1},
publisher = {BioMed Central Ltd},
abstract = {Background: Abscisic acid (ABA) regulates key plant processes, including seed germination, dormancy, and abiotic stress responses. While its physiological role in germination is well-documented, the molecular mechanisms are still poorly understood. To address this, we analyzed transcriptomic and metabolomic changes in ABA-treated germinating barley (Hordeum vulgare) embryos. To map ABA-responsive gene expression across embryonic tissues, we employed the Visium Spatial Transcriptomics (10× Genomics). This approach, which remains technically challenging to be applied in plant tissues, enabled the precise localization of gene expression across six embryo regions, offering insights into tissue-specific expression patterns that cannot be resolved by traditional RNA-seq. Results: Transcriptomic analysis indicated that ABA acts primarily as a germination repressor. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses linked ABA-inhibited genes to energy metabolism, lignin biosynthesis, cell wall organization, and photosynthesis, while induced genes were associated with environmental adaptation and phytohormone signaling. Differentially expressed genes (DEGs) correlated with metabolites involved in phytohormone pathways, including gibberellins, jasmonates, brassinosteroids, salicylic acid, auxins, and ABA metabolism. Comparisons with developing seed transcriptomes suggested an ABA-associated gene expression signature in embryos. Spatial transcriptomics technique made possible the precise identification of ABA-induced transcriptional changes within distinct embryonic tissues. Conclusions: Integrating transcriptomics, metabolomics and spatial transcriptomics defined the molecular signature of ABA-induced modulation of phytohormonal crosstalk, energy metabolism, and tissue-specific gene activity in germinating seeds. The successful use of spatial transcriptomics adds a novel layer of resolution for understanding tissue-specific ABA responses during barley seed germination. These findings offer new insights into the ABA role in seed germination and potential strategies for enhancing crop resilience. © The Author(s) 2025.},
note = {3},
keywords = {},
pubstate = {published},
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}
Background: Abscisic acid (ABA) regulates key plant processes, including seed germination, dormancy, and abiotic stress responses. While its physiological role in germination is well-documented, the molecular mechanisms are still poorly understood. To address this, we analyzed transcriptomic and metabolomic changes in ABA-treated germinating barley (Hordeum vulgare) embryos. To map ABA-responsive gene expression across embryonic tissues, we employed the Visium Spatial Transcriptomics (10× Genomics). This approach, which remains technically challenging to be applied in plant tissues, enabled the precise localization of gene expression across six embryo regions, offering insights into tissue-specific expression patterns that cannot be resolved by traditional RNA-seq. Results: Transcriptomic analysis indicated that ABA acts primarily as a germination repressor. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses linked ABA-inhibited genes to energy metabolism, lignin biosynthesis, cell wall organization, and photosynthesis, while induced genes were associated with environmental adaptation and phytohormone signaling. Differentially expressed genes (DEGs) correlated with metabolites involved in phytohormone pathways, including gibberellins, jasmonates, brassinosteroids, salicylic acid, auxins, and ABA metabolism. Comparisons with developing seed transcriptomes suggested an ABA-associated gene expression signature in embryos. Spatial transcriptomics technique made possible the precise identification of ABA-induced transcriptional changes within distinct embryonic tissues. Conclusions: Integrating transcriptomics, metabolomics and spatial transcriptomics defined the molecular signature of ABA-induced modulation of phytohormonal crosstalk, energy metabolism, and tissue-specific gene activity in germinating seeds. The successful use of spatial transcriptomics adds a novel layer of resolution for understanding tissue-specific ABA responses during barley seed germination. These findings offer new insights into the ABA role in seed germination and potential strategies for enhancing crop resilience. © The Author(s) 2025.
Collin, A.; Matkowski, H.; Sybilska, E.; Biantari, A.; Król, O.; Daszkowska-Golec, A.
ABA-induced alternative splicing drives transcriptomic reprogramming for drought tolerance in barley Journal Article
In: BMC Plant Biology, vol. 25, no. 1, 2025, ISSN: 14712229, (8).
@article{2-s2.0-105002977867,
title = {ABA-induced alternative splicing drives transcriptomic reprogramming for drought tolerance in barley},
author = { A. Collin and H. Matkowski and E. Sybilska and A. Biantari and O. Król and A. Daszkowska-Golec},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-105002977867&doi=10.1186%2Fs12870-025-06485-y&partnerID=40&md5=b7eec62f52dd10660a07e19696033fc2},
doi = {10.1186/s12870-025-06485-y},
issn = {14712229},
year = {2025},
date = {2025-01-01},
journal = {BMC Plant Biology},
volume = {25},
number = {1},
publisher = {BioMed Central Ltd},
abstract = {Background: Abscisic acid (ABA) is a phytohormone that mediates plant responses to drought stress by regulating stomatal conductance, gene expression, and photosynthetic efficiency. Although ABA-induced stress priming has shown the potential to improve drought tolerance, the molecular mechanisms underlying ABA pretreatment effects remain poorly understood. This study aimed to determine how ABA pre-treatment at the booting stage influences physiological and molecular responses to drought at the heading stage in barley. Results: The ABA-treated plants exhibited earlier stomatal closure, increased expression of ABA-responsive genes (HvNCED1; HvBG8; and HvA22), and maintained higher chlorophyll levels under drought conditions. Photosynthetic parameters, including photosystem II activity, electron transport rate, and the number of active reaction centers, were preserved in ABA-pretreated plants compared with drought-only plants. Transcriptomic analysis revealed that ABA pre-treatment primed plants for faster activation of stress-responsive pathways, with enhanced expression of genes related to chromatin modifications, RNA metabolism, and ABA signaling during drought. Importantly, Alternative splicing (AS) and isoform switching were significantly amplified in ABA-pretreated plants, underscoring a unique molecular mechanism of ABA priming that enhances drought resilience. Post-stress recovery analysis revealed a greater number of differentially expressed genes (DEGs) and alternatively spliced transcripts (DAS) in ABA-pretreated plants, particularly those involved in chromatin organization and photosynthesis. Physiological analyses demonstrated that time- and dose-optimized ABA applications improved yield parameters, including grain weight and seed area, while mitigating spike sterility under drought conditions. Conclusions: This study demonstrates that ABA pretreatment enhances drought resilience in barley by triggering early stomatal closure, preserving chlorophyll content, and maintaining photosynthetic performance under water stress. At the molecular level, ABA priming accelerates stress-response pathways, promoting alternative splicing, isoform switching, and chromatin modifications that enable transcriptome plasticity. These processes facilitate faster recovery and sustain critical yield components, such as spike number and grain weight, when ABA is applied at optimized timing and concentrations. While large-scale ABA application poses challenges, this study provides a framework for breeding and agronomic strategies to mimic ABA effects, offering a practical path to enhance drought tolerance and yield stability in barley. © The Author(s) 2025.},
note = {8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Background: Abscisic acid (ABA) is a phytohormone that mediates plant responses to drought stress by regulating stomatal conductance, gene expression, and photosynthetic efficiency. Although ABA-induced stress priming has shown the potential to improve drought tolerance, the molecular mechanisms underlying ABA pretreatment effects remain poorly understood. This study aimed to determine how ABA pre-treatment at the booting stage influences physiological and molecular responses to drought at the heading stage in barley. Results: The ABA-treated plants exhibited earlier stomatal closure, increased expression of ABA-responsive genes (HvNCED1; HvBG8; and HvA22), and maintained higher chlorophyll levels under drought conditions. Photosynthetic parameters, including photosystem II activity, electron transport rate, and the number of active reaction centers, were preserved in ABA-pretreated plants compared with drought-only plants. Transcriptomic analysis revealed that ABA pre-treatment primed plants for faster activation of stress-responsive pathways, with enhanced expression of genes related to chromatin modifications, RNA metabolism, and ABA signaling during drought. Importantly, Alternative splicing (AS) and isoform switching were significantly amplified in ABA-pretreated plants, underscoring a unique molecular mechanism of ABA priming that enhances drought resilience. Post-stress recovery analysis revealed a greater number of differentially expressed genes (DEGs) and alternatively spliced transcripts (DAS) in ABA-pretreated plants, particularly those involved in chromatin organization and photosynthesis. Physiological analyses demonstrated that time- and dose-optimized ABA applications improved yield parameters, including grain weight and seed area, while mitigating spike sterility under drought conditions. Conclusions: This study demonstrates that ABA pretreatment enhances drought resilience in barley by triggering early stomatal closure, preserving chlorophyll content, and maintaining photosynthetic performance under water stress. At the molecular level, ABA priming accelerates stress-response pathways, promoting alternative splicing, isoform switching, and chromatin modifications that enable transcriptome plasticity. These processes facilitate faster recovery and sustain critical yield components, such as spike number and grain weight, when ABA is applied at optimized timing and concentrations. While large-scale ABA application poses challenges, this study provides a framework for breeding and agronomic strategies to mimic ABA effects, offering a practical path to enhance drought tolerance and yield stability in barley. © The Author(s) 2025.
Kurowska, M. M.; Janiak, A.; Sitko, K.; Potocka, I. W.; Gajecka, M.; Sybilska, E.; Płociniczak, T.; Lip, S.; Rynkiewicz, M.; Wiecha, K.; Nawrot, M.; Daszkowska-Golec, A.; Szarejko, I.
Functional analysis of HvSNAC1 in stomatal dynamics and drought adaptation Journal Article
In: Journal of Applied Genetics, vol. 66, no. 4, pp. 817-840, 2025, ISSN: 12341983, (2).
@article{2-s2.0-105000822237,
title = {Functional analysis of HvSNAC1 in stomatal dynamics and drought adaptation},
author = { M.M. Kurowska and A. Janiak and K. Sitko and I.W. Potocka and M. Gajecka and E. Sybilska and T. Płociniczak and S. Lip and M. Rynkiewicz and K. Wiecha and M. Nawrot and A. Daszkowska-Golec and I. Szarejko},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-105000822237&doi=10.1007%2Fs13353-025-00956-6&partnerID=40&md5=4f1c1e05e8d23b1110047753a55c71e5},
doi = {10.1007/s13353-025-00956-6},
issn = {12341983},
year = {2025},
date = {2025-01-01},
journal = {Journal of Applied Genetics},
volume = {66},
number = {4},
pages = {817-840},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Drought stress can damage crop growth and lead to a decline in yield, thereby affecting food security, especially in regions vulnerable to climate change. SNAC1 (stress-responsive NAC1), the NAC transcription factor family member, plays a crucial role in stomatal movement regulation. Effective regulation of stomatal movement is essential for protecting plants from water loss during adverse conditions. Our hypothesis revolves around altering HvSNAC1 activity by introducing a point mutation in its encoding gene, thereby influencing stomatal dynamics in barley. Two TILLING mutants, each harboring missense mutations in the NAC domain, exhibited higher stomatal density after drought stress compared to the parent cultivar ‘Sebastian’. These mutants also demonstrated distinct patterns of ABA-induced stomatal movement compared to the wild-type (WT). To delve deeper, we conducted a comprehensive analysis of the transcriptomes of these mutants and the parent cultivar ‘Sebastian’ under both optimal watering conditions and 10 days of drought stress treatment. We identified differentially expressed genes (DEGs) between the mutants and WT plants under control and drought conditions. Furthermore, we pinpointed DEGs specifically expressed in both mutants under drought conditions. Our experiments revealed that the cis-regulatory motif CACG, previously identified in Arabidopsis and rice, is recognized by HvSNAC1 in vitro. Enrichment analysis led to the identification of the cell wall organization category and potential target genes, such as HvEXPA8 (expansin 8), HvXTH (xyloglucan endotransglucosylase/hydrolase), and HvPAE9 (pectin acetylesterase 9), suggesting their regulation by HvSNAC1. These findings suggest that HvSNAC1 may play a role in regulating genes associated with stomatal density, size and reopening. © The Author(s) 2025.},
note = {2},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Drought stress can damage crop growth and lead to a decline in yield, thereby affecting food security, especially in regions vulnerable to climate change. SNAC1 (stress-responsive NAC1), the NAC transcription factor family member, plays a crucial role in stomatal movement regulation. Effective regulation of stomatal movement is essential for protecting plants from water loss during adverse conditions. Our hypothesis revolves around altering HvSNAC1 activity by introducing a point mutation in its encoding gene, thereby influencing stomatal dynamics in barley. Two TILLING mutants, each harboring missense mutations in the NAC domain, exhibited higher stomatal density after drought stress compared to the parent cultivar ‘Sebastian’. These mutants also demonstrated distinct patterns of ABA-induced stomatal movement compared to the wild-type (WT). To delve deeper, we conducted a comprehensive analysis of the transcriptomes of these mutants and the parent cultivar ‘Sebastian’ under both optimal watering conditions and 10 days of drought stress treatment. We identified differentially expressed genes (DEGs) between the mutants and WT plants under control and drought conditions. Furthermore, we pinpointed DEGs specifically expressed in both mutants under drought conditions. Our experiments revealed that the cis-regulatory motif CACG, previously identified in Arabidopsis and rice, is recognized by HvSNAC1 in vitro. Enrichment analysis led to the identification of the cell wall organization category and potential target genes, such as HvEXPA8 (expansin 8), HvXTH (xyloglucan endotransglucosylase/hydrolase), and HvPAE9 (pectin acetylesterase 9), suggesting their regulation by HvSNAC1. These findings suggest that HvSNAC1 may play a role in regulating genes associated with stomatal density, size and reopening. © The Author(s) 2025.
2024
Sybilska, E.; Collin, A.; Haddadi, B. Sadat; Mur, L. A. J.; Beckmann, M.; Guo, Wenb.; Simpson, C. G.; Daszkowska-Golec, A.
The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare) Journal Article
In: Scientific Reports, vol. 14, no. 1, 2024, ISSN: 20452322, (6).
@article{2-s2.0-85200584410,
title = {The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)},
author = { E. Sybilska and A. Collin and B. Sadat Haddadi and L.A.J. Mur and M. Beckmann and Wenb. Guo and C.G. Simpson and A. Daszkowska-Golec},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85200584410&doi=10.1038%2fs41598-024-69373-9&partnerID=40&md5=d5f2e91f2189b921fc34a90f5683b1a6},
doi = {10.1038/s41598-024-69373-9},
issn = {20452322},
year = {2024},
date = {2024-01-01},
journal = {Scientific Reports},
volume = {14},
number = {1},
publisher = {Nature Research},
abstract = {To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways. © The Author(s) 2024.},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways. © The Author(s) 2024.
2023
Sybilska, E.; Daszkowska-Golec, A.
A complex signaling trio in seed germination: Auxin-JA-ABA Journal Article
In: Trends in Plant Science, vol. 28, no. 8, pp. 873-875, 2023, ISSN: 13601385, (2).
@article{2-s2.0-85159675297,
title = {A complex signaling trio in seed germination: Auxin-JA-ABA},
author = { E. Sybilska and A. Daszkowska-Golec},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85159675297&doi=10.1016%2fj.tplants.2023.05.003&partnerID=40&md5=f414487d157463562f983b990aaa08dc},
doi = {10.1016/j.tplants.2023.05.003},
issn = {13601385},
year = {2023},
date = {2023-01-01},
journal = {Trends in Plant Science},
volume = {28},
number = {8},
pages = {873-875},
publisher = {Elsevier Ltd},
abstract = {Recently. Mei et al. discovered the molecular mechanism behind the synergistic action of auxins and jasmonates in enhancing the role of abscisic acid (ABA) in seed germination. They found that JASMONATE-ZIM DOMAIN (JAZ) proteins interact with AUXIN RESPONSE FACTOR (ARF)-16 to mediate auxin–jasmonic acid (JA) crosstalk. Furthermore, they revealed that ARF16 interacts with ABSCISIC ACID INSENSITIVE (ABI)-5 and positively modulates ABA responses at seed germination. © 2023 Elsevier Ltd},
note = {2},
keywords = {},
pubstate = {published},
tppubtype = {article}
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Recently. Mei et al. discovered the molecular mechanism behind the synergistic action of auxins and jasmonates in enhancing the role of abscisic acid (ABA) in seed germination. They found that JASMONATE-ZIM DOMAIN (JAZ) proteins interact with AUXIN RESPONSE FACTOR (ARF)-16 to mediate auxin–jasmonic acid (JA) crosstalk. Furthermore, they revealed that ARF16 interacts with ABSCISIC ACID INSENSITIVE (ABI)-5 and positively modulates ABA responses at seed germination. © 2023 Elsevier Ltd
Sybilska, E.; Daszkowska-Golec, A.
Alternative splicing in ABA signaling during seed germination Journal Article
In: Frontiers in Plant Science, vol. 14, 2023, ISSN: 1664462X, (1).
@article{2-s2.0-85151957441,
title = {Alternative splicing in ABA signaling during seed germination},
author = { E. Sybilska and A. Daszkowska-Golec},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151957441&doi=10.3389%2ffpls.2023.1144990&partnerID=40&md5=f92e1abe3eeaba0a984d519942c54672},
doi = {10.3389/fpls.2023.1144990},
issn = {1664462X},
year = {2023},
date = {2023-01-01},
journal = {Frontiers in Plant Science},
volume = {14},
publisher = {Frontiers Media S.A.},
abstract = {Seed germination is an essential step in a plant’s life cycle. It is controlled by complex physiological, biochemical, and molecular mechanisms and external factors. Alternative splicing (AS) is a co-transcriptional mechanism that regulates gene expression and produces multiple mRNA variants from a single gene to modulate transcriptome diversity. However, little is known about the effect of AS on the function of generated protein isoforms. The latest reports indicate that alternative splicing (AS), the relevant mechanism controlling gene expression, plays a significant role in abscisic acid (ABA) signaling. In this review, we present the current state of the art about the identified AS regulators and the ABA-related changes in AS during seed germination. We show how they are connected with the ABA signaling and the seed germination process. We also discuss changes in the structure of the generated AS isoforms and their impact on the functionality of the generated proteins. Also, we point out that the advances in sequencing technology allow for a better explanation of the role of AS in gene regulation by more accurate detection of AS events and identification of full-length splicing isoforms. Copyright © 2023 Sybilska and Daszkowska-Golec.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Seed germination is an essential step in a plant’s life cycle. It is controlled by complex physiological, biochemical, and molecular mechanisms and external factors. Alternative splicing (AS) is a co-transcriptional mechanism that regulates gene expression and produces multiple mRNA variants from a single gene to modulate transcriptome diversity. However, little is known about the effect of AS on the function of generated protein isoforms. The latest reports indicate that alternative splicing (AS), the relevant mechanism controlling gene expression, plays a significant role in abscisic acid (ABA) signaling. In this review, we present the current state of the art about the identified AS regulators and the ABA-related changes in AS during seed germination. We show how they are connected with the ABA signaling and the seed germination process. We also discuss changes in the structure of the generated AS isoforms and their impact on the functionality of the generated proteins. Also, we point out that the advances in sequencing technology allow for a better explanation of the role of AS in gene regulation by more accurate detection of AS events and identification of full-length splicing isoforms. Copyright © 2023 Sybilska and Daszkowska-Golec.