• mgr Kamila Godel-Jędrychowska
Stanowisko: Biolog
Jednostka: Biuro ds. Infrastruktury Badawczo-Dydaktycznej WNP
Adres: 40-032 Katowice, ul. Jagiellońska 28
Piętro: III
Numer pokoju: B-306
Telefon: (32) 2009 446
E-mail: kamila.godel-jedrychowska@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 57209237319
Publikacje z bazy Scopus
2023
Godel-Jędrychowska, K.; Milewska-Hendel, A.; Sala, K.; Baranski, R.; Kurczyńska, E. U.
The Impact of Gold Nanoparticles on Somatic Embryogenesis Using the Example of Arabidopsis thaliana Journal Article
In: International Journal of Molecular Sciences, vol. 24, no. 12, 2023, ISSN: 16616596, (1).
@article{2-s2.0-85164024170,
title = {The Impact of Gold Nanoparticles on Somatic Embryogenesis Using the Example of Arabidopsis thaliana},
author = { K. Godel-Jędrychowska and A. Milewska-Hendel and K. Sala and R. Baranski and E.U. Kurczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85164024170&doi=10.3390%2fijms241210356&partnerID=40&md5=e32d3618b3f386a03753326e24a779bc},
doi = {10.3390/ijms241210356},
issn = {16616596},
year = {2023},
date = {2023-01-01},
journal = {International Journal of Molecular Sciences},
volume = {24},
number = {12},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
abstract = {Although the influence of nanoparticles (NPs) on developmental processes is better understood, little is known about their impact on somatic embryogenesis (SE). This process involves changes in the direction of cell differentiation. Thus, studying the effect of NPs on SE is essential to reveal their impact on cell fate. This study aimed to examine the influence of gold nanoparticles (Au NPs) with different surface charges on the SE of 35S:BBM Arabidopsis thaliana, with particular emphasis on the spatiotemporal localization of pectic arabinogalactan proteins (AGPs) and extensin epitopes in cells changing the direction of their differentiation. The results show that under the influence of nanoparticles, the explant cells of 35S:BBM Arabidopsis thaliana seedling origin did not enter the path of SE. Bulges and the formation of organ-like structures were observed in these explants, in contrast to the control, where somatic embryos developed. Additionally, spatiotemporal changes in the chemical composition of the cell walls during the culture were observed. Under the influence of Au NPs, the following effects were observed: (1) explant cells did not enter the SE pathway, (2) the impacts of Au NPs with different surface charges on the explants were variable, and (3) the compositions of the analyzed pectic AGPs and extensin epitopes were diverse in the cells with different developmental programs: SE (control) and non-SE (treated with Au NPs). © 2023 by the authors.},
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Although the influence of nanoparticles (NPs) on developmental processes is better understood, little is known about their impact on somatic embryogenesis (SE). This process involves changes in the direction of cell differentiation. Thus, studying the effect of NPs on SE is essential to reveal their impact on cell fate. This study aimed to examine the influence of gold nanoparticles (Au NPs) with different surface charges on the SE of 35S:BBM Arabidopsis thaliana, with particular emphasis on the spatiotemporal localization of pectic arabinogalactan proteins (AGPs) and extensin epitopes in cells changing the direction of their differentiation. The results show that under the influence of nanoparticles, the explant cells of 35S:BBM Arabidopsis thaliana seedling origin did not enter the path of SE. Bulges and the formation of organ-like structures were observed in these explants, in contrast to the control, where somatic embryos developed. Additionally, spatiotemporal changes in the chemical composition of the cell walls during the culture were observed. Under the influence of Au NPs, the following effects were observed: (1) explant cells did not enter the SE pathway, (2) the impacts of Au NPs with different surface charges on the explants were variable, and (3) the compositions of the analyzed pectic AGPs and extensin epitopes were diverse in the cells with different developmental programs: SE (control) and non-SE (treated with Au NPs). © 2023 by the authors.
Kurczyńska, E. U.; Godel-Jędrychowska, K.
Apoplastic and Symplasmic Markers of Somatic Embryogenesis Journal Article
In: Plants, vol. 12, no. 10, 2023, ISSN: 22237747, (1).
@article{2-s2.0-85160546790,
title = {Apoplastic and Symplasmic Markers of Somatic Embryogenesis},
author = { E.U. Kurczyńska and K. Godel-Jędrychowska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160546790&doi=10.3390%2fplants12101951&partnerID=40&md5=a9124cfb5c9e46d3c91f66bb674ec020},
doi = {10.3390/plants12101951},
issn = {22237747},
year = {2023},
date = {2023-01-01},
journal = {Plants},
volume = {12},
number = {10},
publisher = {MDPI},
abstract = {Somatic embryogenesis (SE) is a process that scientists have been trying to understand for many years because, on the one hand, it is a manifestation of the totipotency of plant cells, so it enables the study of the mechanisms regulating this process, and, on the other hand, it is an important method of plant propagation. Using SE in basic research and in practice is invaluable. This article describes the latest, but also historical, information on changes in the chemical composition of the cell wall during the transition of cells from the somatic to embryogenic state, and the importance of symplasmic communication during SE. Among wall chemical components, different pectic, AGP, extensin epitopes, and lipid transfer proteins have been discussed as potential apoplastic markers of explant cells during the acquisition of embryogenic competence. The role of symplasmic communication/isolation during SE has also been discussed, paying particular attention to the formation of symplasmic domains within and between cells that carry out different developmental processes. Information about the number and functionality of plasmodesmata (PD) and callose deposition as the main player in symplasmic isolation has also been presented. © 2023 by the authors.},
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Somatic embryogenesis (SE) is a process that scientists have been trying to understand for many years because, on the one hand, it is a manifestation of the totipotency of plant cells, so it enables the study of the mechanisms regulating this process, and, on the other hand, it is an important method of plant propagation. Using SE in basic research and in practice is invaluable. This article describes the latest, but also historical, information on changes in the chemical composition of the cell wall during the transition of cells from the somatic to embryogenic state, and the importance of symplasmic communication during SE. Among wall chemical components, different pectic, AGP, extensin epitopes, and lipid transfer proteins have been discussed as potential apoplastic markers of explant cells during the acquisition of embryogenic competence. The role of symplasmic communication/isolation during SE has also been discussed, paying particular attention to the formation of symplasmic domains within and between cells that carry out different developmental processes. Information about the number and functionality of plasmodesmata (PD) and callose deposition as the main player in symplasmic isolation has also been presented. © 2023 by the authors.
2022
Godel-Jędrychowska, K.; Kurczyńska, E. U.
In: Postepy Biochemii, vol. 68, no. 1, pp. 3-14, 2022, ISSN: 00325422.
@article{2-s2.0-85130021087,
title = {Symplasmic communication – supracellular system of regulation of cell differentiation [Komunikacja symplastowa – nadkomórkowy system regulacji różnicowania komórek]},
author = { K. Godel-Jędrychowska and E.U. Kurczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130021087&doi=10.18388%2fpb.2021_402&partnerID=40&md5=77a2c95d259e78cde3ec672c20f56a23},
doi = {10.18388/pb.2021_402},
issn = {00325422},
year = {2022},
date = {2022-01-01},
journal = {Postepy Biochemii},
volume = {68},
number = {1},
pages = {3-14},
publisher = {Polish Biochemical Society},
abstract = {Plasmodesmata (PD), discovered more than 120 years ago, are still a mystery about their role in regulating plant cell differentiation. Research in recent years has verified our idea about the structure of PD and their function in the exchange of information between cells of the plant body. The involvement of PD in the movement of proteins, including transcription factors, hormones, and various types of RNA, indicates that they play an important role in regulating cell differentiation. The movement of molecules through PD is called symplasmic communication, and its limitations or absence are an essential element in controlling the direction of cell differentiation. © 2022, Polish Biochemical Society. All rights reserved.},
keywords = {},
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Plasmodesmata (PD), discovered more than 120 years ago, are still a mystery about their role in regulating plant cell differentiation. Research in recent years has verified our idea about the structure of PD and their function in the exchange of information between cells of the plant body. The involvement of PD in the movement of proteins, including transcription factors, hormones, and various types of RNA, indicates that they play an important role in regulating cell differentiation. The movement of molecules through PD is called symplasmic communication, and its limitations or absence are an essential element in controlling the direction of cell differentiation. © 2022, Polish Biochemical Society. All rights reserved.
Wróbel-Marek, J.; Godel-Jędrychowska, K.; Kurczyńska, E. U.
Analysis of the Distribution of Symplasmic Tracers During Zygotic and Somatic Embryogenesis Book Chapter
In: vol. 2457, pp. 351-365, Humana Press Inc., 2022, ISSN: 10643745, (1).
@inbook{2-s2.0-85127259487,
title = {Analysis of the Distribution of Symplasmic Tracers During Zygotic and Somatic Embryogenesis},
author = { J. Wróbel-Marek and K. Godel-Jędrychowska and E.U. Kurczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85127259487&doi=10.1007%2f978-1-0716-2132-5_24&partnerID=40&md5=88838265f3213a2f034d9271d7ae0977},
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journal = {Methods in Molecular Biology},
volume = {2457},
pages = {351-365},
publisher = {Humana Press Inc.},
abstract = {Plasmodesmata (PD) are membraneous channels that span cell walls of adjacent cells to establish the symplasm. These connections are unique to plants and enable the cell-to-cell exchange of information via the symplasm. However, not every plant cell is connected to its neighbor. Absence of PD and lack of communication (symplasmic isolation) are important regulators of cell differentiation. To determine cell-to-cell symplasmic connectivity, the distribution of fluorescent tracers can be analyzed. Here, we describe in detail the entire procedure for conducting such analysis using fluorescence and confocal microscopy to study molecular fluxes in fluorescence recovery after photobleaching (FRAP) experiments. Studies using fluorochromes and fluorescent-labeled dextrans successfully inform the degree of symplasmic connectivity between cells in zygotic and somatic embryos. Small molecules, such as water and ions, travel through PD but also transcription factors and different types of RNA. Studies of symplasmic communication are important to determine the spatio-temporal correlation between cell differentiation and the exchange of information between cells. This information is necessary to determine the role of symplasmic communication during embryogenesis, which is a very important stage in plant development and morphogenesis. © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.},
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Plasmodesmata (PD) are membraneous channels that span cell walls of adjacent cells to establish the symplasm. These connections are unique to plants and enable the cell-to-cell exchange of information via the symplasm. However, not every plant cell is connected to its neighbor. Absence of PD and lack of communication (symplasmic isolation) are important regulators of cell differentiation. To determine cell-to-cell symplasmic connectivity, the distribution of fluorescent tracers can be analyzed. Here, we describe in detail the entire procedure for conducting such analysis using fluorescence and confocal microscopy to study molecular fluxes in fluorescence recovery after photobleaching (FRAP) experiments. Studies using fluorochromes and fluorescent-labeled dextrans successfully inform the degree of symplasmic connectivity between cells in zygotic and somatic embryos. Small molecules, such as water and ions, travel through PD but also transcription factors and different types of RNA. Studies of symplasmic communication are important to determine the spatio-temporal correlation between cell differentiation and the exchange of information between cells. This information is necessary to determine the role of symplasmic communication during embryogenesis, which is a very important stage in plant development and morphogenesis. © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Godel-Jędrychowska, K.; Franke, T.; Kurczyńska, E. U.
Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata Book Chapter
In: vol. 2457, pp. 75-94, Humana Press Inc., 2022, ISSN: 10643745, (1).
@inbook{2-s2.0-85127232400,
title = {Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata},
author = { K. Godel-Jędrychowska and T. Franke and E.U. Kurczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85127232400&doi=10.1007%2f978-1-0716-2132-5_4&partnerID=40&md5=3711c952241d412c12bd45ad8b056633},
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year = {2022},
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journal = {Methods in Molecular Biology},
volume = {2457},
pages = {75-94},
publisher = {Humana Press Inc.},
abstract = {Array tomography (AT) is a new high-throughput imaging method for high-resolution imaging of ultrastructure and for 3-D reconstruction of cells and organelles. Here, we describe the entire procedure for obtaining a spatial image of the distribution of plasmodesmata (PD). As example, the protocol is applied here to reconstruct the number and arrangement of PD between cells undergoing differentiation during Arabidopsis somatic embryogenesis. © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.},
note = {1},
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Array tomography (AT) is a new high-throughput imaging method for high-resolution imaging of ultrastructure and for 3-D reconstruction of cells and organelles. Here, we describe the entire procedure for obtaining a spatial image of the distribution of plasmodesmata (PD). As example, the protocol is applied here to reconstruct the number and arrangement of PD between cells undergoing differentiation during Arabidopsis somatic embryogenesis. © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Godel-Jędrychowska, K.; Kurczyńska, E. U.
In: Functional Plant Biology, vol. 49, no. 2, pp. 186-200, 2022, ISSN: 14454408, (1).
@article{2-s2.0-85120543114,
title = {Qualitative and quantitative analyses of the plasmodesmata that accompany cell fate changes during the somatic embryogenesis of Arabidopsis thaliana},
author = { K. Godel-Jędrychowska and E.U. Kurczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120543114&doi=10.1071%2fFP21243&partnerID=40&md5=279c14467f5420c126514ad4cbb3959d},
doi = {10.1071/FP21243},
issn = {14454408},
year = {2022},
date = {2022-01-01},
journal = {Functional Plant Biology},
volume = {49},
number = {2},
pages = {186-200},
publisher = {CSIRO},
abstract = {Plasmodesmata (PD) are cytoplasmic and membrane-lined microchannels that enable symplasmic communication in plants, which is involved in the regulation of cell differentiation. The presented results emphasise the qualitative and quantitative analyses of PD, which are the basis of the symplasmic communication. The cells that initiate various development programmes create symplasmic domains that are characterised by different degrees of symplasmic communication. Changes in symplasmic communication are caused by the presence or absence of PD and/or the ability of signals to move through them. In the presented studies, somatic embryogenesis was used to describe the characteristics of the PD within and between the symplasmic domains in explants of the Arabidopsis thaliana (L.) Heynh ecotype Columbia-0 and 35S:BBM transgenic line. Transmission electron microscopy was used to describe the cells that regain totipotency/pluripotency during somatic embryogenesis, as well as the number and shape of the PD in the different symplasmic domains of the explants and somatic embryos. Array tomography was used to create a 3D reconstruction of the protodermal cells of the somatic embryos with particular emphasis on the PD distribution in the cell walls. The results showed that there were different frequencies of the PD within and between the symplasmic domain that emerges during somatic embryogenesis and between the Col-0 and 35S:BBM somatic embryos with regard to the differences in the shape of the PD. © 2022},
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Plasmodesmata (PD) are cytoplasmic and membrane-lined microchannels that enable symplasmic communication in plants, which is involved in the regulation of cell differentiation. The presented results emphasise the qualitative and quantitative analyses of PD, which are the basis of the symplasmic communication. The cells that initiate various development programmes create symplasmic domains that are characterised by different degrees of symplasmic communication. Changes in symplasmic communication are caused by the presence or absence of PD and/or the ability of signals to move through them. In the presented studies, somatic embryogenesis was used to describe the characteristics of the PD within and between the symplasmic domains in explants of the Arabidopsis thaliana (L.) Heynh ecotype Columbia-0 and 35S:BBM transgenic line. Transmission electron microscopy was used to describe the cells that regain totipotency/pluripotency during somatic embryogenesis, as well as the number and shape of the PD in the different symplasmic domains of the explants and somatic embryos. Array tomography was used to create a 3D reconstruction of the protodermal cells of the somatic embryos with particular emphasis on the PD distribution in the cell walls. The results showed that there were different frequencies of the PD within and between the symplasmic domain that emerges during somatic embryogenesis and between the Col-0 and 35S:BBM somatic embryos with regard to the differences in the shape of the PD. © 2022
2021
Kurczyńska, E. U.; Godel-Jędrychowska, K.; Sala, K.; Milewska-Hendel, A.
Nanoparticles—plant interaction: What we know, where we are? Journal Article
In: Applied Sciences (Switzerland), vol. 11, no. 12, 2021, ISSN: 20763417, (7).
@article{2-s2.0-85108635869,
title = {Nanoparticles—plant interaction: What we know, where we are?},
author = { E.U. Kurczyńska and K. Godel-Jędrychowska and K. Sala and A. Milewska-Hendel},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108635869&doi=10.3390%2fapp11125473&partnerID=40&md5=5739d81152baef30b3dc2ed0c4b3f0f7},
doi = {10.3390/app11125473},
issn = {20763417},
year = {2021},
date = {2021-01-01},
journal = {Applied Sciences (Switzerland)},
volume = {11},
number = {12},
publisher = {MDPI AG},
abstract = {In recent years; the interaction of nanoparticles (NPs) with plants has been intensively studied. Therefore, more and more aspects related to both the positive and negative impact of NP on plants are well described. This article focuses on two aspects of NP interaction with plants. The first is a summary of the current knowledge on NP migration through the roots into the plant body, in particular, the role of the cell wall. The second aspect summarizes the current knowledge of the participation of the symplast, including the plasmodesmata (PD), in the movement of NP within the plant body. We highlight the gaps in our knowledge of the plant–NP interactions; paying attention to the need for future studies to explain the mechanisms that regulate the composition of the cell wall and the functioning of the PD under the influence of NP. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {7},
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pubstate = {published},
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In recent years; the interaction of nanoparticles (NPs) with plants has been intensively studied. Therefore, more and more aspects related to both the positive and negative impact of NP on plants are well described. This article focuses on two aspects of NP interaction with plants. The first is a summary of the current knowledge on NP migration through the roots into the plant body, in particular, the role of the cell wall. The second aspect summarizes the current knowledge of the participation of the symplast, including the plasmodesmata (PD), in the movement of NP within the plant body. We highlight the gaps in our knowledge of the plant–NP interactions; paying attention to the need for future studies to explain the mechanisms that regulate the composition of the cell wall and the functioning of the PD under the influence of NP. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Oleszkiewicz, T.; Klimek-Chodacka, M.; Kruczek, M.; Godel-Jędrychowska, K.; Sala, K.; Milewska-Hendel, A.; Zubko, M.; Kurczyńska, E. U.; Qi, Y.; Baranski, R.
Inhibition of carotenoid biosynthesis by crispr/cas9triggers cell wall remodelling in carrot Journal Article
In: International Journal of Molecular Sciences, vol. 22, no. 12, 2021, ISSN: 16616596, (5).
@article{2-s2.0-85108065626,
title = {Inhibition of carotenoid biosynthesis by crispr/cas9triggers cell wall remodelling in carrot},
author = { T. Oleszkiewicz and M. Klimek-Chodacka and M. Kruczek and K. Godel-Jędrychowska and K. Sala and A. Milewska-Hendel and M. Zubko and E.U. Kurczyńska and Y. Qi and R. Baranski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108065626&doi=10.3390%2fijms22126516&partnerID=40&md5=08bdce802b200c7f39ad88ac5c476848},
doi = {10.3390/ijms22126516},
issn = {16616596},
year = {2021},
date = {2021-01-01},
journal = {International Journal of Molecular Sciences},
volume = {22},
number = {12},
publisher = {MDPI},
abstract = {Recent data indicate that modifications to carotenoid biosynthesis pathway in plants alter the expression of genes affecting chemical composition of the cell wall. Phytoene synthase (PSY) is a rate limiting factor of carotenoid biosynthesis and it may exhibit species-specific and or-gan-specific roles determined by the presence of psy paralogous genes, the importance of which often remains unrevealed. Thus, the aim of this work was to elaborate the roles of two psy paralogs in a model system and to reveal biochemical changes in the cell wall of psy knockout mutants. For this purpose, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (Cas9) proteins (CRISPR/Cas9) vectors were introduced to carotenoid-rich carrot (Daucus carota) callus cells in order to induce mutations in the psy1 and psy2 genes. Gene sequencing, expression analysis, and carotenoid content analysis revealed that the psy2 gene is critical for carotenoid bio-synthesis in this model and its knockout blocks carotenogenesis. The psy2 knockout also decreased the expression of the psy1 paralog. Immunohistochemical staining of the psy2 mutant cells showed altered composition of arabinogalactan proteins, pectins, and extensins in the mutant cell walls. In particular, low-methylesterified pectins were abundantly present in the cell walls of carote-noid-rich callus in contrast to the carotenoid-free psy2 mutant. Transmission electron microscopy revealed altered plastid transition to amyloplasts instead of chromoplasts. The results demonstrate for the first time that the inhibited biosynthesis of carotenoids triggers the cell wall remodelling. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {5},
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pubstate = {published},
tppubtype = {article}
}
Recent data indicate that modifications to carotenoid biosynthesis pathway in plants alter the expression of genes affecting chemical composition of the cell wall. Phytoene synthase (PSY) is a rate limiting factor of carotenoid biosynthesis and it may exhibit species-specific and or-gan-specific roles determined by the presence of psy paralogous genes, the importance of which often remains unrevealed. Thus, the aim of this work was to elaborate the roles of two psy paralogs in a model system and to reveal biochemical changes in the cell wall of psy knockout mutants. For this purpose, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (Cas9) proteins (CRISPR/Cas9) vectors were introduced to carotenoid-rich carrot (Daucus carota) callus cells in order to induce mutations in the psy1 and psy2 genes. Gene sequencing, expression analysis, and carotenoid content analysis revealed that the psy2 gene is critical for carotenoid bio-synthesis in this model and its knockout blocks carotenogenesis. The psy2 knockout also decreased the expression of the psy1 paralog. Immunohistochemical staining of the psy2 mutant cells showed altered composition of arabinogalactan proteins, pectins, and extensins in the mutant cell walls. In particular, low-methylesterified pectins were abundantly present in the cell walls of carote-noid-rich callus in contrast to the carotenoid-free psy2 mutant. Transmission electron microscopy revealed altered plastid transition to amyloplasts instead of chromoplasts. The results demonstrate for the first time that the inhibited biosynthesis of carotenoids triggers the cell wall remodelling. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Godel-Jędrychowska, K.; Kulińska-Łukaszek, K.; Kurczyńska, E. U.
Similarities and Differences in the GFP Movement in the Zygotic and Somatic Embryos of Arabidopsis Journal Article
In: Frontiers in Plant Science, vol. 12, 2021, ISSN: 1664462X, (1).
@article{2-s2.0-85107550909,
title = {Similarities and Differences in the GFP Movement in the Zygotic and Somatic Embryos of Arabidopsis},
author = { K. Godel-Jędrychowska and K. Kulińska-Łukaszek and E.U. Kurczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107550909&doi=10.3389%2ffpls.2021.649806&partnerID=40&md5=4892959110fd3fda6312f875f34a1e9c},
doi = {10.3389/fpls.2021.649806},
issn = {1664462X},
year = {2021},
date = {2021-01-01},
journal = {Frontiers in Plant Science},
volume = {12},
publisher = {Frontiers Media S.A.},
abstract = {Intercellular signaling during embryo patterning is not well understood and the role of symplasmic communication has been poorly considered. The correlation between the symplasmic domains and the development of the embryo organs/tissues during zygotic embryogenesis has only been described for a few examples, including Arabidopsis. How this process occurs during the development of somatic embryos (SEs) is still unknown. The aim of these studies was to answer the question: do SEs have a restriction in symplasmic transport depending on the developmental stage that is similar to their zygotic counterparts? The studies included an analysis of the GFP distribution pattern as expressed under diverse promoters in zygotic embryos (ZEs) and SEs. The results of the GFP distribution in the ZEs and SEs showed that 1/the symplasmic domains between the embryo organs and tissues in the SEs was similar to those in the ZEs and 2/the restriction in symplasmic transport in the SEs was correlated with the developmental stage and was similar to the one in their zygotic counterparts, however, with the spatio-temporal differences and different PDs SEL value between these two types of embryos. © Copyright © 2021 Godel-Jędrychowska, Kulińska-Łukaszek and Kurczyńska.},
note = {1},
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Intercellular signaling during embryo patterning is not well understood and the role of symplasmic communication has been poorly considered. The correlation between the symplasmic domains and the development of the embryo organs/tissues during zygotic embryogenesis has only been described for a few examples, including Arabidopsis. How this process occurs during the development of somatic embryos (SEs) is still unknown. The aim of these studies was to answer the question: do SEs have a restriction in symplasmic transport depending on the developmental stage that is similar to their zygotic counterparts? The studies included an analysis of the GFP distribution pattern as expressed under diverse promoters in zygotic embryos (ZEs) and SEs. The results of the GFP distribution in the ZEs and SEs showed that 1/the symplasmic domains between the embryo organs and tissues in the SEs was similar to those in the ZEs and 2/the restriction in symplasmic transport in the SEs was correlated with the developmental stage and was similar to the one in their zygotic counterparts, however, with the spatio-temporal differences and different PDs SEL value between these two types of embryos. © Copyright © 2021 Godel-Jędrychowska, Kulińska-Łukaszek and Kurczyńska.
Godel-Jędrychowska, K.; Kulińska-Łukaszek, K.; Horstman, A.; Soriano, M.; Li, M.; Małota, K.; Boutilier, K.; Kurczyńska, E. U.
Symplasmic isolation marks cell fate changes during somatic embryogenesis Journal Article
In: Journal of Experimental Botany, vol. 71, no. 9, pp. 2612-2628, 2021, ISSN: 00220957, (18).
@article{2-s2.0-85096384288,
title = {Symplasmic isolation marks cell fate changes during somatic embryogenesis},
author = { K. Godel-Jędrychowska and K. Kulińska-Łukaszek and A. Horstman and M. Soriano and M. Li and K. Małota and K. Boutilier and E.U. Kurczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096384288&doi=10.1093%2fJXB%2fERAA041&partnerID=40&md5=6d7f312b2384090f1b146b9ae888268e},
doi = {10.1093/JXB/ERAA041},
issn = {00220957},
year = {2021},
date = {2021-01-01},
journal = {Journal of Experimental Botany},
volume = {71},
number = {9},
pages = {2612-2628},
publisher = {Oxford University Press},
abstract = {Cell-to-cell signalling is a major mechanism controlling plant morphogenesis. Transport of signalling molecules through plasmodesmata is one way in which plants promote or restrict intercellular signalling over short distances. Plasmodesmata are membrane-lined pores between cells that regulate the intercellular flow of signalling molecules through changes in their size, creating symplasmic fields of connected cells. Here we examine the role of plasmodesmata and symplasmic communication in the establishment of plant cell totipotency, using somatic embryo induction from Arabidopsis explants as a model system. Cell-to-cell communication was evaluated using fluorescent tracers, supplemented with histological and ultrastructural analysis, and correlated with expression of a WOX2 embryo reporter. We showed that embryogenic cells are isolated symplasmically from non-embryogenic cells regardless of the explant type (immature zygotic embryos or seedlings) and inducer system (2;4-dichlorophenoxyacetic acid or the BABY BOOM (BBM) transcription factor), but that the symplasmic domains in different explants differ with respect to the maximum size of molecule capable of moving through the plasmodesmata. Callose deposition in plasmodesmata preceded WOX2 expression in future sites of somatic embryo development, but later was greatly reduced in WOX2expressing domains. Callose deposition was also associated with a decrease DR5 auxin response in embryogenic tissue. Treatment of explants with the callose biosynthesis inhibitor 2-deoxy-D-glucose supressed somatic embryo formation in all three systems studied, and also blocked the observed decrease in DR5 expression. Together these data suggest that callose deposition at plasmodesmata is required for symplasmic isolation and establishment of cell totipotency in Arabidopsis. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.},
note = {18},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Cell-to-cell signalling is a major mechanism controlling plant morphogenesis. Transport of signalling molecules through plasmodesmata is one way in which plants promote or restrict intercellular signalling over short distances. Plasmodesmata are membrane-lined pores between cells that regulate the intercellular flow of signalling molecules through changes in their size, creating symplasmic fields of connected cells. Here we examine the role of plasmodesmata and symplasmic communication in the establishment of plant cell totipotency, using somatic embryo induction from Arabidopsis explants as a model system. Cell-to-cell communication was evaluated using fluorescent tracers, supplemented with histological and ultrastructural analysis, and correlated with expression of a WOX2 embryo reporter. We showed that embryogenic cells are isolated symplasmically from non-embryogenic cells regardless of the explant type (immature zygotic embryos or seedlings) and inducer system (2;4-dichlorophenoxyacetic acid or the BABY BOOM (BBM) transcription factor), but that the symplasmic domains in different explants differ with respect to the maximum size of molecule capable of moving through the plasmodesmata. Callose deposition in plasmodesmata preceded WOX2 expression in future sites of somatic embryo development, but later was greatly reduced in WOX2expressing domains. Callose deposition was also associated with a decrease DR5 auxin response in embryogenic tissue. Treatment of explants with the callose biosynthesis inhibitor 2-deoxy-D-glucose supressed somatic embryo formation in all three systems studied, and also blocked the observed decrease in DR5 expression. Together these data suggest that callose deposition at plasmodesmata is required for symplasmic isolation and establishment of cell totipotency in Arabidopsis. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.
2019
Godel-Jędrychowska, K.; Maćkowska, K.; Kurczyńska, E. U.; Grzebelus, E.
Composition of the reconstituted cell wall in protoplast-derived cells of daucus is affected by phytosulfokine (PSK) Journal Article
In: International Journal of Molecular Sciences, vol. 20, no. 21, 2019, ISSN: 16616596, (6).
@article{2-s2.0-85074563909,
title = {Composition of the reconstituted cell wall in protoplast-derived cells of daucus is affected by phytosulfokine (PSK)},
author = { K. Godel-Jędrychowska and K. Maćkowska and E.U. Kurczyńska and E. Grzebelus},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074563909&doi=10.3390%2fijms20215490&partnerID=40&md5=df2521002a27351a86ff912e9e654f86},
doi = {10.3390/ijms20215490},
issn = {16616596},
year = {2019},
date = {2019-01-01},
journal = {International Journal of Molecular Sciences},
volume = {20},
number = {21},
publisher = {MDPI AG},
abstract = {Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation. It was shown that PSK stimulated and enhanced cell divisions in protoplast cultures of several species leading to callus and proembryogenic mass formation. Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process. So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells. Even less is known about the correlation between wall regeneration and the presence of PSK during the protoplast culture. Three Daucus taxa, including the cultivated carrot, were analyzed during protoplast regeneration. Several antibodies directed against wall components (anti-pectin: LM19; LM20; anti-AGP: JIM4; JIM8; JIM13 and anti-extensin: JIM12) were used. The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation. It was shown that PSK stimulated and enhanced cell divisions in protoplast cultures of several species leading to callus and proembryogenic mass formation. Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process. So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells. Even less is known about the correlation between wall regeneration and the presence of PSK during the protoplast culture. Three Daucus taxa, including the cultivated carrot, were analyzed during protoplast regeneration. Several antibodies directed against wall components (anti-pectin: LM19; LM20; anti-AGP: JIM4; JIM8; JIM13 and anti-extensin: JIM12) were used. The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
Piński, A.; Betekhtin, A.; Sala, K.; Godel-Jędrychowska, K.; Kurczyńska, E. U.; Hasterok, R.
Hydroxyproline-rich glycoproteins as markers of temperature stress in the leaves of brachypodium distachyon Journal Article
In: International Journal of Molecular Sciences, vol. 20, no. 10, 2019, ISSN: 16616596, (12).
@article{2-s2.0-85066960172,
title = {Hydroxyproline-rich glycoproteins as markers of temperature stress in the leaves of brachypodium distachyon},
author = { A. Piński and A. Betekhtin and K. Sala and K. Godel-Jędrychowska and E.U. Kurczyńska and R. Hasterok},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066960172&doi=10.3390%2fijms20102571&partnerID=40&md5=83367841b3aa36bbcbe19816364135ad},
doi = {10.3390/ijms20102571},
issn = {16616596},
year = {2019},
date = {2019-01-01},
journal = {International Journal of Molecular Sciences},
volume = {20},
number = {10},
publisher = {MDPI AG},
abstract = {Plants frequently encounter diverse abiotic stresses, one of which is environmental thermal stress. To cope with these stresses, plants have developed a range of mechanisms, including altering the cell wall architecture, which is facilitated by the arabinogalactan proteins (AGP) and extensins (EXT). In order to characterise the localisation of the epitopes of the AGP and EXT, which are induced by the stress connected with a low (4◦ C) or a high (40◦ C) temperature, in the leaves of Brachypodium distachyon, we performed immunohistochemical analyses using the antibodies that bind to selected AGP (JIM8; JIM13; JIM16; LM2 and MAC207), pectin/AGP (LM6) as well as EXT (JIM11; JIM12 and JIM20). The analyses of the epitopes of the AGP indicated their presence in the phloem and in the inner bundle sheath (JIM8; JIM13; JIM16 and LM2). The JIM16 epitope was less abundant in the leaves from the low or high temperature compared to the control leaves. The LM2 epitope was more abundant in the leaves that had been subjected to the high temperatures. In the case of JIM13 and MAC207, no changes were observed at the different temperatures. The epitopes of the EXT were primarily observed in the mesophyll and xylem cells of the major vascular bundle (JIM11; JIM12 and JIM20) and no correlation was observed between the presence of the epitopes and the temperature stress. We also analysed changes in the level of transcript accumulation of some of the genes encoding EXT, EXT-like receptor kinases and AGP in the response to the temperature stress. In both cases, although we observed the upregulation of the genes encoding AGP in stressed plants, the changes were more pronounced at the high temperature. Similar changes were observed in the expression profiles of the EXT and EXT-like receptor kinase genes. Our findings may be relevant for genetic engineering of plants with increased resistance to the temperature stress. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {12},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Plants frequently encounter diverse abiotic stresses, one of which is environmental thermal stress. To cope with these stresses, plants have developed a range of mechanisms, including altering the cell wall architecture, which is facilitated by the arabinogalactan proteins (AGP) and extensins (EXT). In order to characterise the localisation of the epitopes of the AGP and EXT, which are induced by the stress connected with a low (4◦ C) or a high (40◦ C) temperature, in the leaves of Brachypodium distachyon, we performed immunohistochemical analyses using the antibodies that bind to selected AGP (JIM8; JIM13; JIM16; LM2 and MAC207), pectin/AGP (LM6) as well as EXT (JIM11; JIM12 and JIM20). The analyses of the epitopes of the AGP indicated their presence in the phloem and in the inner bundle sheath (JIM8; JIM13; JIM16 and LM2). The JIM16 epitope was less abundant in the leaves from the low or high temperature compared to the control leaves. The LM2 epitope was more abundant in the leaves that had been subjected to the high temperatures. In the case of JIM13 and MAC207, no changes were observed at the different temperatures. The epitopes of the EXT were primarily observed in the mesophyll and xylem cells of the major vascular bundle (JIM11; JIM12 and JIM20) and no correlation was observed between the presence of the epitopes and the temperature stress. We also analysed changes in the level of transcript accumulation of some of the genes encoding EXT, EXT-like receptor kinases and AGP in the response to the temperature stress. In both cases, although we observed the upregulation of the genes encoding AGP in stressed plants, the changes were more pronounced at the high temperature. Similar changes were observed in the expression profiles of the EXT and EXT-like receptor kinase genes. Our findings may be relevant for genetic engineering of plants with increased resistance to the temperature stress. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.