@article{2-s2.0-85167662362,

title = {Promotive effect of phytosulfokine - peptide growth factor - on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn},

author = { M. Zaranek and R. Pérez-Pérez and A. Milewska-Hendel and A. Betekhtin and E. Grzebelus},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85167662362&doi=10.1186%2fs12870-023-04402-9&partnerID=40&md5=2294ecea0866096bfd9e83b09d510d07},

doi = {10.1186/s12870-023-04402-9},

issn = {14712229},

year  = {2023},

date = {2023-01-01},

journal = {BMC Plant Biology},

volume = {23},

number = {1},

pages = {385-},

publisher = {BioMed Central Ltd},

abstract = {Background: Fagopyrum tataricum (Tartary buckwheat) is a valuable crop of great nutritional importance due to its high level of bioactive compounds. Excellent opportunities to obtain plants with the high level or the desired profile of valuable metabolites may be provided by in vitro cultures. Among known in vitro techniques, protoplast technology is an exciting tool for genetic manipulation to improve crop traits. In that context, protoplast fusion may be applied to generate hybrid cells between different species of Fagopyrum. To apply protoplast cultures to the aforementioned approaches in this research, we established the protoplast-to-plant system in Tartary buckwheat. Results: In this work, cellulase and pectinase activity enabled protoplast isolation from non-morphogenic and morphogenic callus (MC), reaching, on average, 2.3 × 106 protoplasts per g of fresh weight. However, to release protoplasts from hypocotyls, the key step was the application of driselase in the enzyme mixture. We showed that colony formation could be induced after protoplast embedding in agarose compared to the alginate matrix. Protoplasts cultured in a medium based on Kao and Michayluk supplemented with phytosulfokine (PSK) rebuilt cell walls, underwent repeated mitotic division, formed aggregates, which consequently led to callus formation. Plating efficiency, expressing the number of cell aggregate formed, in 10-day-old protoplast cultures varied from 14% for morphogenic callus to 30% for hypocotyls used as a protoplast source. However plant regeneration via somatic embryogenesis and organogenesis occurred only during the cultivation of MC-derived protoplasts. Conclusions: This study demonstrated that the applied protoplast isolation approach facilitated the recovery of viable protoplasts. Moreover, the embedding of protoplasts in an agarose matrix and supplementation of a culture medium with PSK effectively stimulated cell division and further development of Tartary buckwheat protoplast cultures along with the plant regeneration. Together, these results provide the first evidence of developing a protoplast-to-plant system from the MC of Fagopyrum tataricum used as source material. These findings suggest that Tartary buckwheat’s protoplast cultures have potential implications for the species’ somatic hybridization and genetic improvement. © 2023, BioMed Central Ltd., part of Springer Nature.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85163116120,

title = {Efficient and rapid system of plant regeneration via protoplast cultures of Fagopyrum esculentum Moench},

author = { M. Zaranek and R. Pérez-Pérez and A. Milewska-Hendel and E. Grzebelus and A. Betekhtin},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85163116120&doi=10.1007%2fs11240-023-02542-2&partnerID=40&md5=095db46aa63cec8daad37b85250fd60e},

doi = {10.1007/s11240-023-02542-2},

issn = {01676857},

year  = {2023},

date = {2023-01-01},

journal = {Plant Cell, Tissue and Organ Culture},

volume = {154},

number = {3},

pages = {673-687},

publisher = {Springer Science and Business Media B.V.},

abstract = {In the present study, a high yield of isolated protoplasts from the agronomically important crop Fagopyrum esculentum was obtained by applying a mixture of cellulase, pectolyase, and driselase. We demonstrated that the yield of morphogenic callus-derived protoplasts was 1 × 106 protoplasts per g of fresh tissue. For hypocotyls used as the protoplast source, the number of released cells was twice lower. The protoplasts, embedded in an agarose matrix and cultured in a modified Kao and Michayluk media supplemented with phytosulfokine, re-enter the cell cycle and start to develop, forming microcalli. The plating efficiency was about 20% in the case of hypocotyl- and morphogenic callus-derived protoplasts. For plant regeneration, the medium was supplemented with different combinations of cytokinin. Somatic embryogenesis and organogenesis occur during the cultivation of the protoplast-derived tissues, depending on the applied protoplast source. For the first time, an effective protoplast-to-plant system for F. esculentum has been developed. © 2023, The Author(s).},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85164024170,

title = {The Impact of Gold Nanoparticles on Somatic Embryogenesis Using the Example of Arabidopsis thaliana},

author = { K. Godel-Jędrychowska and A. Milewska-Hendel and K. Sala and R. Baranski and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85164024170&doi=10.3390%2fijms241210356&partnerID=40&md5=e32d3618b3f386a03753326e24a779bc},

doi = {10.3390/ijms241210356},

issn = {16616596},

year  = {2023},

date = {2023-01-01},

journal = {International Journal of Molecular Sciences},

volume = {24},

number = {12},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {Although the influence of nanoparticles (NPs) on developmental processes is better understood, little is known about their impact on somatic embryogenesis (SE). This process involves changes in the direction of cell differentiation. Thus, studying the effect of NPs on SE is essential to reveal their impact on cell fate. This study aimed to examine the influence of gold nanoparticles (Au NPs) with different surface charges on the SE of 35S:BBM Arabidopsis thaliana, with particular emphasis on the spatiotemporal localization of pectic arabinogalactan proteins (AGPs) and extensin epitopes in cells changing the direction of their differentiation. The results show that under the influence of nanoparticles, the explant cells of 35S:BBM Arabidopsis thaliana seedling origin did not enter the path of SE. Bulges and the formation of organ-like structures were observed in these explants, in contrast to the control, where somatic embryos developed. Additionally, spatiotemporal changes in the chemical composition of the cell walls during the culture were observed. Under the influence of Au NPs, the following effects were observed: (1) explant cells did not enter the SE pathway, (2) the impacts of Au NPs with different surface charges on the explants were variable, and (3) the compositions of the analyzed pectic AGPs and extensin epitopes were diverse in the cells with different developmental programs: SE (control) and non-SE (treated with Au NPs). © 2023 by the authors.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85127510342,

title = {Morphological, Histological and Ultrastructural Changes in Hordeum vulgare (L.) Roots That Have Been Exposed to Negatively Charged Gold Nanoparticles},

author = { A. Milewska-Hendel and W. Gepfert and M. Zubko and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85127510342&doi=10.3390%2fapp12073265&partnerID=40&md5=32e845d5c1fbcde8dd8ea646d8006723},

doi = {10.3390/app12073265},

issn = {20763417},

year  = {2022},

date = {2022-01-01},

journal = {Applied Sciences (Switzerland)},

volume = {12},

number = {7},

publisher = {MDPI},

abstract = {In recent years, there has been an impressive development of nanotechnology. This has resulted in the increasing release of nanomaterials (NM) into the environment, thereby causing the risk of an uncontrolled impact on living organisms, including plants. More studies indicated the biotoxic effect of NM on plants, including crops. The interaction of nanoparticles (NP) with food crops is extremely important as they are a link to the food chain. The objective of this study was to investigate the effect of negatively charged gold nanoparticles (-) AuNP (at two concentrations; 25 µg/mL or 50 µg/mL) on barley (Hordeum vulgare L.) root development. Morphological, histological and ultrastructural analyses (with the use of stereomicroscope; bright filed microscope and transmission electron microscope) revealed that regardless of the concentration, (-) AuNP did not enter into the plant body. However, the dose of (-) AuNP proved to be important for the plant’s response because different morphological, histological and ultrastructural changes were observed in the treated roots. The NP treatment caused: red root colouration, a local increase in the root diameter and a decreased formation of the root hair cells (on morphological level), damage to the rhizodermal cells, vacuolisation of the cortical cells, a detachment of the cell files between the cortical cells, atypical divisions of the cells, disorder of the meristem organisation (on the histological level), the appearance of periplasmic space, numerous vesicles and multivesicular bodies, electron-dense spots in cytoplasm, alterations in the structure of the mitochondria, breakdown of the tonoplast and the plasmalemma (on the ultrastructural level). © 2022 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85115042302,

title = {Gold nanoparticles‐induced modifications in cell wall composition in barley roots},

author = { A. Milewska-Hendel and K. Sala and W. Gepfert and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115042302&doi=10.3390%2fcells10081965&partnerID=40&md5=22a9870ffeb8d79b61ecfc26ec5c8c5d},

doi = {10.3390/cells10081965},

issn = {20734409},

year  = {2021},

date = {2021-01-01},

journal = {Cells},

volume = {10},

number = {8},

publisher = {MDPI},

abstract = {The increased use of nanoparticles (NP) in different industries inevitably results in their release into the environment. In such conditions, plants come into direct contact with NP. Knowledge about the uptake of NP by plants and their effect on different developmental processes is still insufficient. Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP). The analyses were performed using the immunohistological method and fluorescence microscopy. The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85108635869,

title = {Nanoparticles—plant interaction: What we know, where we are?},

author = { E.U. Kurczyńska and K. Godel-Jędrychowska and K. Sala and A. Milewska-Hendel},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108635869&doi=10.3390%2fapp11125473&partnerID=40&md5=5739d81152baef30b3dc2ed0c4b3f0f7},

doi = {10.3390/app11125473},

issn = {20763417},

year  = {2021},

date = {2021-01-01},

journal = {Applied Sciences (Switzerland)},

volume = {11},

number = {12},

publisher = {MDPI AG},

abstract = {In recent years; the interaction of nanoparticles (NPs) with plants has been intensively studied. Therefore, more and more aspects related to both the positive and negative impact of NP on plants are well described. This article focuses on two aspects of NP interaction with plants. The first is a summary of the current knowledge on NP migration through the roots into the plant body, in particular, the role of the cell wall. The second aspect summarizes the current knowledge of the participation of the symplast, including the plasmodesmata (PD), in the movement of NP within the plant body. We highlight the gaps in our knowledge of the plant–NP interactions; paying attention to the need for future studies to explain the mechanisms that regulate the composition of the cell wall and the functioning of the PD under the influence of NP. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85108065626,

title = {Inhibition of carotenoid biosynthesis by crispr/cas9triggers cell wall remodelling in carrot},

author = { T. Oleszkiewicz and M. Klimek-Chodacka and M. Kruczek and K. Godel-Jędrychowska and K. Sala and A. Milewska-Hendel and M. Zubko and E.U. Kurczyńska and Y. Qi and R. Baranski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108065626&doi=10.3390%2fijms22126516&partnerID=40&md5=08bdce802b200c7f39ad88ac5c476848},

doi = {10.3390/ijms22126516},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {12},

publisher = {MDPI},

abstract = {Recent data indicate that modifications to carotenoid biosynthesis pathway in plants alter the expression of genes affecting chemical composition of the cell wall. Phytoene synthase (PSY) is a rate limiting factor of carotenoid biosynthesis and it may exhibit species-specific and or-gan-specific roles determined by the presence of psy paralogous genes, the importance of which often remains unrevealed. Thus, the aim of this work was to elaborate the roles of two psy paralogs in a model system and to reveal biochemical changes in the cell wall of psy knockout mutants. For this purpose, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (Cas9) proteins (CRISPR/Cas9) vectors were introduced to carotenoid-rich carrot (Daucus carota) callus cells in order to induce mutations in the psy1 and psy2 genes. Gene sequencing, expression analysis, and carotenoid content analysis revealed that the psy2 gene is critical for carotenoid bio-synthesis in this model and its knockout blocks carotenogenesis. The psy2 knockout also decreased the expression of the psy1 paralog. Immunohistochemical staining of the psy2 mutant cells showed altered composition of arabinogalactan proteins, pectins, and extensins in the mutant cell walls. In particular, low-methylesterified pectins were abundantly present in the cell walls of carote-noid-rich callus in contrast to the carotenoid-free psy2 mutant. Transmission electron microscopy revealed altered plastid transition to amyloplasts instead of chromoplasts. The results demonstrate for the first time that the inhibited biosynthesis of carotenoids triggers the cell wall remodelling. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85097811034,

title = {Cell wall epitopes in grasses of different novel ecosystem habitats on post-industrial sites},

author = { A. Milewska-Hendel and D. Chmura and K. Wyrwał and E.U. Kurczyńska and A. Kompała-Bąba and A.M. Jagodziński and G. Woźniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097811034&doi=10.1002%2fldr.3786&partnerID=40&md5=b91f178a948f1d4383600818a1c0b390},

doi = {10.1002/ldr.3786},

issn = {10853278},

year  = {2021},

date = {2021-01-01},

journal = {Land Degradation and Development},

volume = {32},

number = {4},

pages = {1680-1694},

publisher = {John Wiley and Sons Ltd},

abstract = {The post-industrial habitats provide previously unknown conditions for plant and vegetation development. We asked the question: do the extreme soil substrate conditions cause differences in chemical composition of cell walls of leaves in three grass species: Calamagrostis epigejos (L.) Roth, Phragmites australis (Cav.) Trin. ex Steud, and Molinia caerulea (L.) Moench? With the use of immunohistochemical methods we determined the spatial distribution of selected pectic and AGP (arabinogalactan proteins) epitopes within leaf tissues of grass species growing in two industrial and control (meadow) habitats. Some post-industrial habitats increase biodiversity. Fast adaptation and divergence cause phenotypic changes. This process has been recently recorded and is called human-induced rapid evolutionary changes. The levels of pectins and arabinogalactan proteins increased on the post-industrial habitats in comparison to the control sites. On control habitat, pectic epitope with galactan chain residues (recognised by the LM5 antibody) was not detected in cell walls of Calamagrostis epigejos leaves, but was abundantly present in Phragmites australis leaves. The pectic epitope with arabinan residues (recognized by LM6 antibody) was less represented in cell walls of Molinia caerulea leaves. AGP epitope, recognised by LM2 antibody, was abundant only in Ph. australis. In Molinia caerulea this epitope was not detected. The AGP epitope recognised by JIM13 antibody was observed in all analysed species. The results obtained revealed the response of grass individuals to different environments: a) varied responses to different soil substratum conditions (novel ecosystems), and b) that this reaction is species-specific with respect to the analysed cell wall components. © 2020 John Wiley & Sons, Ltd.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85063231438,

title = {The development of a hairless phenotype in barley roots treated with gold nanoparticles is accompanied by changes in the symplasmic communication},

author = { A. Milewska-Hendel and W. Witek and A. Rypień and M. Zubko and R. Baranski and D. Stróż and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063231438&doi=10.1038%2fs41598-019-41164-7&partnerID=40&md5=458a420e10e45c07c747ba10f47a76f7},

doi = {10.1038/s41598-019-41164-7},

issn = {20452322},

year  = {2019},

date = {2019-01-01},

journal = {Scientific Reports},

volume = {9},

number = {1},

publisher = {Nature Publishing Group},

abstract = {Uptake of water and nutrients by roots affects the ontogenesis of the whole plant. Nanoparticles, e.g. gold nanoparticles, have a broad range of applications in many fields which leads to the transfer of these materials into the environment. Thus, the understanding of their impact on the growth and development of the root system is an emerging issue. During our studies on the effect of positively charged gold nanoparticles on the barley roots, a hairless phenotype was found. We investigated whether this phenotype correlates with changes in symplasmic communication, which is an important factor that regulates, among others, differentiation of the rhizodermis into hair and non-hair cells. The results showed no restriction in symplasmic communication in the treated roots, in contrast to the control roots, in which the trichoblasts and atrichoblasts were symplasmically isolated during their differentiation. Moreover, differences concerning the root morphology, histology, ultrastructure and the cell wall composition were detected between the control and the treated roots. These findings suggest that the harmful effect of nanoparticles on plant growth may, among others, consist in disrupting the symplasmic communication/isolation, which leads to the development of a hairless root phenotype, thus limiting the functioning of the roots. © 2019, The Author(s).},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85068749385,

title = {Aluminum alters the histology and pectin cell wall composition of barley roots},

author = { J. Jaskowiak and J. Kwaśniewska and A. Milewska-Hendel and E.U. Kurczyńska and M.E. Szurman-Zubrzycka and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068749385&doi=10.3390%2fijms20123039&partnerID=40&md5=4ea918ecebc6ab755c98c545ce00eb10},

doi = {10.3390/ijms20123039},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {12},

publisher = {MDPI AG},

abstract = {Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils. Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity. In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure. We targeted four different pectic epitopes recognized by LM5, LM6, LM19, and LM20 antibodies using an immunocytochemical approach. Since Al becomes available and toxic to plants in acidic soils, we performed our analyses on barley roots that had been grown in acidic conditions (pH 4.0) with and without Al and in control conditions (pH 6.0). Differences connected with the presence and distribution of the pectic epitopes between the control and Al-treated roots were observed. In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present. Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount. Based on the presented results, it was concluded that methyl-esterified HG can be a marker for newly arising cell walls. Additionally, histological changes were detected in the roots grown under Al exposure. Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed. The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions. The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85061604241,

title = {Stability and instability processes in the calli of Fagopyrum tataricum that have different morphogenic potentials},

author = { A. Betekhtin and A. Piński and A. Milewska-Hendel and E.U. Kurczyńska and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061604241&doi=10.1007%2fs11240-019-01575-w&partnerID=40&md5=645591d8a95ce3e965e098b879213554},

doi = {10.1007/s11240-019-01575-w},

issn = {01676857},

year  = {2019},

date = {2019-01-01},

journal = {Plant Cell, Tissue and Organ Culture},

volume = {137},

number = {2},

pages = {343-357},

publisher = {Springer Netherlands},

abstract = {The morphogenic callus (MC) of Fagopyrum tataricum contains a large amount of flavonoids, especially rutin, and exhibits a high level of antioxidant activity. A non-morphogenic callus (NC) may appear on the surface of the MC after two to three years of cultivation and is then subjected to a consistently high level of oxidative stress. The elucidation of the molecular background of this instability is essential for gaining a better understanding of the somaclonal variation mechanisms in tissue cultures that have different morphogenic potentials. Thus, in this study we show that continuous oxidative stress in a NC might be connected with a rapid senescence process and as a result, in the upregulation of the genes that are connected with the telomere complexity, ethylene biosynthesis and the expression of DNA methyltransferases. Moreover, we analysed the presence of the hydroxyproline-rich glycoproteins in the calli and demonstrated the differences between the MC and NC. The LM2 antibody can be useful as a marker of the cells in the MC that are embryogenically determined, while the MAC207 antibody seems to be a positive marker of a MC as its signal was absent in the NC. This study also provides the first report on the effect of trichostatin A on the DNA methyltransferases and demethylases in a MC. © 2019, The Author(s).},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85064930296,

title = {Effect of nanoparticles surface charge on the Arabidopsis thaliana (L.) roots development and their movement into the root cells and protoplasts},

author = { A. Milewska-Hendel and M. Zubko and D. Stróż and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064930296&doi=10.3390%2fijms20071650&partnerID=40&md5=1bd3b99f9fc8b4827645a064403650d4},

doi = {10.3390/ijms20071650},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {7},

publisher = {MDPI AG},

abstract = {Increasing usage of gold nanoparticles (AuNPs) in different industrial areas inevitably leads to their release into the environment. Thus, living organisms, including plants, may be exposed to a direct contact with nanoparticles (NPs). Despite the growing amount of research on this topic, our knowledge about NPs uptake by plants and their influence on different developmental processes is still insufficient. The first physical barrier for NPs penetration to the plant body is a cell wall which protects cytoplasm from external factors and environmental stresses. The absence of a cell wall may facilitate the internalization of various particles including NPs. Our studies have shown that AuNPs, independently of their surface charge, did not cross the cell wall of Arabidopsis thaliana (L.) roots. However, the research carried out with using light and transmission electron microscope revealed that AuNPs with different surface charge caused diverse changes in the root’s histology and ultrastructure. Therefore, we verified whether this is only the wall which protects cells against particles penetration and for this purpose we used protoplasts culture. It has been shown that plasma membrane (PM) is not a barrier for positively charged (+) AuNPs and negatively charged (−) AuNPs, which passage to the cell. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85057991726,

title = {Cell wall epitopes and endoploidy as reporters of embryogenic potential in brachypodium distachyon callus culture},

author = { A. Betekhtin and M. Rojek-Jelonek and K. Nowak and A. Piński and A. Milewska-Hendel and E.U. Kurczyńska and J.H. Doonan and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057991726&doi=10.3390%2fijms19123811&partnerID=40&md5=a019f7df04ed76fd5a1440b62abca7ae},

doi = {10.3390/ijms19123811},

issn = {16616596},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Molecular Sciences},

volume = {19},

number = {12},

publisher = {MDPI AG},

abstract = {Effective regeneration of callus tissue into embryos and then into whole plants is essential for plant biotechnology. The embryonic potential is often low and can further decrease with time in culture, which limits the utilisation of calli for transformation procedures and in vitro propagation. In this study, we show that the loss of embryogenic potential in callus cultures of Brachypodium distachyon is progressive over time. Flow cytometry analyses indicated endoploidy levels increased in 60-and 90-day-old calli with effective loss of the 2C DNA content peak in the latter. Analysis of indolic compounds content revealed a decrease in 60-and 90-day-old calli compared to either freshly isolated explants or 30-day-old calli. Immunohistochemical analysis revealed a decrease in arabinogalactan proteins (AGP) signal with the time of culture, but extensin (EXT) epitopes either increased (JIM12 epitopes) or decreased (JIM11 epitopes). The transcript accumulation levels of AGPs and EXTs confirmed these results, with most of AGP and EXT transcripts gradually decreasing. Some chimeric EXT transcripts significantly increased on the 30th day of culture, perhaps because of an increased embryogenic potential. Selected somatic embryogenesis-related genes and cyclins demonstrated a gradual decrease of transcript accumulation for YUCCA (YUC), AINTEGUMENTA-LIKE (AIL), BABY BOOM (BBM), and CLAVATA (CLV3) genes, as well as for most of the cyclins, starting from the 30th day of culture. Notably, WUSCHEL (WUS) transcript was detectable only on the 30th and 60th day and was not detectable in the zygotic embryos and in 90-day-old calli. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85052124909,

title = {Unique chromoplast organisation and carotenoid gene expression in carotenoid-rich carrot callus},

author = { T. Oleszkiewicz and M. Klimek-Chodacka and A. Milewska-Hendel and M. Zubko and D. Stróż and E.U. Kurczyńska and A. Boba and J. Szopa and R. Baranski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052124909&doi=10.1007%2fs00425-018-2988-5&partnerID=40&md5=b60366c39577f353d0cbea077a81b5ba},

doi = {10.1007/s00425-018-2988-5},

issn = {00320935},

year  = {2018},

date = {2018-01-01},

journal = {Planta},

volume = {248},

number = {6},

pages = {1455-1471},

publisher = {Springer Verlag},

abstract = {Main conclusion: The new model orange callus line, similar to carrot root, was rich in carotenoids due to altered expression of some carotenogenesis-associated genes and possessed unique diversity of chromoplast ultrastructure. Callus induced from carrot root segments cultured in vitro is usually pale yellow (p-y) and poor in carotenoids. A unique, non-engineered callus line of dark orange (d-o) colour was developed in this work. The content of carotenoid pigments in d-o callus was at the same level as in an orange carrot storage root and nine-fold higher than in p-y callus. Carotenoids accumulated mainly in abundant crystalline chromoplasts that are also common in carrot root but not in p-y callus. Using transmission electron microscopy, other types of chromoplasts were also found in d-o callus, including membranous chromoplasts rarely identified in plants and not observed in carrot root until now. At the transcriptional level, most carotenogenesis-associated genes were upregulated in d-o callus in comparison to p-y callus, but their expression was downregulated or unchanged when compared to root tissue. Two pathway steps were critical and could explain the massive carotenoid accumulation in this tissue. The geranylgeranyl diphosphate synthase gene involved in the biosynthesis of carotenoid precursors was highly expressed, while the β-carotene hydroxylase gene involved in β-carotene conversion to downstream xanthophylls was highly repressed. Additionally, paralogues of these genes and phytoene synthase were differentially expressed, indicating their tissue-specific roles in carotenoid biosynthesis and metabolism. The established system may serve as a novel model for elucidating plastid biogenesis that coincides with carotenogenesis. © 2018, The Author(s).},

note = {20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85048889878,

title = {5-Azacitidine induces cell death in a tissue culture of brachypodium distachyon},

author = { A. Betekhtin and A. Milewska-Hendel and Ł. Chajec and M. Rojek-Jelonek and K. Nowak and J. Kwaśniewska and E.A. Wolny and E.U. Kurczyńska and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048889878&doi=10.3390%2fijms19061806&partnerID=40&md5=72a6b817774ef0346f00f6530b07a6ac},

doi = {10.3390/ijms19061806},

issn = {16616596},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Molecular Sciences},

volume = {19},

number = {6},

publisher = {MDPI AG},

abstract = {Morphological and histological observations revealed that, at a concentration of 50 µM, 5-azacitidine (5-azaC) totally inhibited the induction of embryogenic masses (EM), while the cultivation of explants (zygotic embryos; ZEs) in the presence of 5 µM of 5-azaC led to the formation of a callus with EM in 10% of the cases. Transmission electron microscopy (TEM) analyzes revealed the presence of the morphological and ultrastructural features that are typical for the vacuolar type of cell death in the callus cells that were treated. A TUNEL assay confirmed the presence of DNA double-strand breaks for the callus cells that had been treated with both 5 and 50 µM 5-azaC concentrations. Analysis of the gene expression of selected cell death markers demonstrated a reduced expression of metacaspase, protein executer 1 (EX1), and thioredoxin (TRX) in the callus cells that had been treated compared to the control culture. The strongest increase in the gene activity was characteristic for glutathione S-transferase (GST). Our studies also included an analysis of the distribution of some arabinogalactan proteins (AGPs) and extensin epitopes, which can be used as markers of cells that are undergoing death in a Brachypodium distachyon tissue culture. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85042854293,

title = {Organ and tissue-specific localisation of selected cell wall epitopes in the zygotic embryo of Brachypodium distachyon},

author = { A. Betekhtin and A. Milewska-Hendel and J. Lusinska and Ł. Chajec and E.U. Kurczyńska and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042854293&doi=10.3390%2fijms19030725&partnerID=40&md5=f697b77194807b245593766f2c341404},

doi = {10.3390/ijms19030725},

issn = {16616596},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Molecular Sciences},

volume = {19},

number = {3},

publisher = {MDPI AG},

abstract = {The plant cell wall shows a great diversity regarding its chemical composition, which may vary significantly even during different developmental stages. In this study, we analysed the distribution of several cell wall epitopes in embryos of Brachypodium distachyon (Brachypodium). We also described the variations in the nucleus shape and the number of nucleoli that occurred in some embryo cells. The use of transmission electron microscopy, and histological and immunolocalisation techniques permitted the distribution of selected arabinogalactan proteins, extensins, pectins, and hemicelluloses on the embryo surface, internal cell compartments, and in the context of the cell wall ultrastructure to be demonstrated. We revealed that the majority of arabinogalactan proteins and extensins were distributed on the cell surface and that pectins were the main component of the seed coat and other parts, such as the mesocotyl cell walls and the radicula. Hemicelluloses were localised in the cell wall and outside of the radicula protodermis, respectively. The specific arrangement of those components may indicate their significance during embryo development and seed germination, thus suggesting the importance of their protective functions. Despite the differences in the cell wall composition, we found that some of the antibodies can be used as markers to identify specific cells and the parts of the developing Brachypodium embryo. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85020376429,

title = {Fate of neutral-charged gold nanoparticles in the roots of the Hordeum vulgare L. cultivar Karat},

author = { A. Milewska-Hendel and M. Zubko and J. Karcz and D. Stróż and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020376429&doi=10.1038%2fs41598-017-02965-w&partnerID=40&md5=491d1da7c9f8fdf8bb31eb6e751eafff},

doi = {10.1038/s41598-017-02965-w},

issn = {20452322},

year  = {2017},

date = {2017-01-01},

journal = {Scientific Reports},

volume = {7},

number = {1},

publisher = {Nature Publishing Group},

abstract = {Nanoparticles (NPs) have a significant impact on the environment and living organisms. The influence of NPs on plants is intensively studied and most of the data indicate that NPs can penetrate into plants. The studies presented here were performed on the roots of Hordeum vulgare L. seedlings using neutral-charge gold nanoparticles (AuNPs) of different sizes. In contrast to the majority of the published data, the results presented here showed that during the culture period, AuNPs: 1/did not enter the root regardless of their size and concentration, 2/that are applied directly into the cells of a root do not move into neighbouring cells. The results that were obtained indicate that in order to extend our knowledge about the mechanisms of the interactions between NPs and plants, further studies including, among others, on different species and a variety of growth conditions are needed. © 2017 The Author(s).},

note = {32},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85014957702,

title = {Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn},

author = { A. Betekhtin and M. Rojek-Jelonek and J. Jaskowiak and A. Milewska-Hendel and J. Kwaśniewska and Y. Kostyukova and E.U. Kurczyńska and N. Rumyantseva and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85014957702&doi=10.1371%2fjournal.pone.0173537&partnerID=40&md5=8a4e7fc7b0fee91c193c03db23eaecb2},

doi = {10.1371/journal.pone.0173537},

issn = {19326203},

year  = {2017},

date = {2017-01-01},

journal = {PLoS ONE},

volume = {12},

number = {3},

publisher = {Public Library of Science},

abstract = {Long-term cultivated Fagopyrum tataricum (L.) Gaertn. (Tartary buckwheat) morphogenic and non-morphogenic callus lines are interesting systems for gaining a better understanding of the mechanisms that are responsible for the genetic stability and instability of a plant tissue culture. In this work, we used histological sections and transmission electron microscopy to identify and describe the morphology of the nuclei of all of the analysed callus lines. We demonstrated that the embryogenic callus cells had prominent round nuclei that did not contain heterochromatin clumps in contrast to the non-morphogenic callus lines, in which we found nuclei that had multiple lobes. Flow cytometry analysis revealed significant differences in the relative DNA content between the analysed calli. All of the analysed morphogenic callus lines had peaks from 2C to 8C as compared to the nonmorphogenic callus lines, whose peaks did not reflect any regular DNA content and exceeded 8C and 16C for the line 6p1 and 16C and 32C for the callus line 10p2A. The results showed that non-morphogenic calli are of an aneuploid nature. The TUNEL test enabled us to visualise the nuclei that had DNA fragmentation in both the morphogenic and non-morphogenic lines. We revealed significantly higher frequencies of positively labelled nuclei in the non-morphogenic lines than in the morphogenic lines. In the case of the morphogenic lines, the highest observed frequency of TUNEL-positive nuclei was 7.7% for lines 2-3. In the non-morphogenic calli, the highest level of DNA damage (68.5%) was revealed in line 6p1. These results clearly indicate greater genome stability in the morphogenic lines. © 2017 Betekhtin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85013892983,

title = {Quantitative and qualitative characteristics of cell wall components and prenyl lipids in the leaves of Tilia x euchlora trees growing under salt stress},

author = { A. Milewska-Hendel and A.H. Baczewska and K. Sala and W. Dmuchowski and P. Bragoszewska and D. Gozdowski and A. Jozwiak and T. Chojnacki and E. Swiezewska and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013892983&doi=10.1371%2fjournal.pone.0172682&partnerID=40&md5=9273a1f5b327a5d5316ca4927ba2751f},

doi = {10.1371/journal.pone.0172682},

issn = {19326203},

year  = {2017},

date = {2017-01-01},

journal = {PLoS ONE},

volume = {12},

number = {2},

publisher = {Public Library of Science},

abstract = {The study was focused on assessing the presence of arabinogalactan proteins (AGPs) and pectins within the cell walls as well as prenyl lipids, sodium and chlorine content in leaves of Tilia x euchlora trees. The leaves that were analyzed were collected from trees with and without signs of damage that were all growing in the same salt stress conditions. The reason for undertaking these investigations was the observations over many years that indicated that there are trees that present a healthy appearance and trees that have visible symptoms of decay in the same habitat. Leaf samples were collected from trees growing in the median strip between roadways that have been intensively salted during the winter season for many years. The sodium content was determined using atomic spectrophotometry, chloride using potentiometric titration and poly-isoprenoids using HPLC/UV. AGPs and pectins were determined using immunohistochemistry methods. The immunohistochemical analysis showed that rhamnogalacturonans I (RG-I) and homogalacturonans were differentially distributed in leaves from healthy trees in contrast to leaves from injured trees. In the case of AGPs, the most visible difference was the presence of the JIM16 epitope. Chemical analyses of sodium and chloride showed that in the leaves from injured trees, the level of these ions was higher than in the leaves from healthy trees. Based on chromatographic analysis, four polyisoprenoid alcohols were identified in the leaves of T. x euchlora. The levels of these lipids were higher in the leaves from healthy trees. The results suggest that the differences that were detected in the apoplast and symplasm may be part of the defensive strategy of T. x euchlora trees to salt stress, which rely on changes in the chemical composition of the cell wall with respect to the pectic and AGP epitopes and an increased synthesis of prenyl lipids. © 2017 Milewska-Hendel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021764617,

title = {Morpho-histological analysis of tomato (Solanum lycopersicum L.) plants after treatment with juglone},

author = { A. Milewska-Hendel and M. Polak and K. Sala and P. Zieleźnik-Rusinowska and R. Gawecki and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021764617&doi=10.5586%2faa.1701&partnerID=40&md5=08218efe0b5c44652666a966d3d5eeee},

doi = {10.5586/aa.1701},

issn = {00650951},

year  = {2017},

date = {2017-01-01},

journal = {Acta Agrobotanica},

volume = {70},

number = {2},

publisher = {Polish Botanical Society},

abstract = {Juglone is a substance that limits plant growth and has a toxic effect on plant development. In this study, we analyzed the influence of juglone at two different concentrations (10-3 M and 10-4 M), which were applied to different parts of Solanum lycopersicum L. plants (root system; stem after decapitation; and surface of a younger leaf or after autografting) for a short period of time (7 days), on the morphology and histology of stems. At a lower concentration, juglone had positive effects on plant growth, which resulted in an increase in interfascicular cambial cell divisions, faster development of a continuous cambium layer along the stem circumference, and development of fibers. Additionally, under the influence of juglone, the number of developing leaves increased and adventitious roots developed. The results are discussed based on the current literature concerning the reaction of plants to juglone and to stress conditions. © The Author(s) 2017.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84997354235,

title = {Spatial distribution of selected chemical cell wall components in the embryogenic callus of brachypodium distachyon},

author = { A. Betekhtin and M. Rojek-Jelonek and A. Milewska-Hendel and R. Gawecki and J. Karcz and E.U. Kurczyńska and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84997354235&doi=10.1371%2fjournal.pone.0167426&partnerID=40&md5=d5dadb0308d02b7c3e39490a13f7fd39},

doi = {10.1371/journal.pone.0167426},

issn = {19326203},

year  = {2016},

date = {2016-01-01},

journal = {PLoS ONE},

volume = {11},

number = {11},

publisher = {Public Library of Science},

abstract = {Brachypodium distachyon L. Beauv. (Brachypodium) is a species that has become an excellent model system for gaining a better understanding of various areas of grass biology and improving plant breeding. Although there are some studies of an in vitro Brachypodium culture including somatic embryogenesis, detailed knowledge of the composition of the main cell wall components in the embryogenic callus in this species is missing. Therefore, using the immunocytochemical approach, we targeted 17 different antigens of which five were against the arabinogalactan proteins (AGP), three were against extensins, six recognised pectic epitopes and two recognised hemicelluloses. These studies were complemented by histological and scanning electron microscopy (SEM) analyses. We revealed that the characteristic cell wall components of Brachypodium embryogenic calli are AGP epitopes that are recognised by the JIM16 and LM2 antibodies, an extensin epitope that is recognised by the JIM11 antibody and a pectic epitopes that is recognised by the LM6 antibody. Furthermore, we demonstrated that AGPs and pectins are the components of the extracellular matrix network in Brachypodium embryogenic culture. Additionally, SEM analysis demonstrated the presence of an extracellular matrix on the surface of the calli cells. In conclusion, the chemical compositions of the cell walls and ECMSN of Brachypodium callus show spatial differences that correlate with the embryogenic character of the cells. Thus, the distribution of pectins, AGPs and hemicelluloses can be used as molecular markers of embryogenic cells. The presented data extends the knowledge about the chemical composition of the embryogenic callus cells of Brachypodium. © 2016 Betekhtin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {21},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85003012274,

title = {Diverse influence of nanoparticles on plant growth with a particular emphasis on crop plants},

author = { A. Milewska-Hendel and R. Gawecki and M. Zubko and D. Stróż and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85003012274&doi=10.5586%2faa.1694&partnerID=40&md5=bda7fd8ffc68b024bdb5d5b9981b0440},

doi = {10.5586/aa.1694},

issn = {00650951},

year  = {2016},

date = {2016-01-01},

journal = {Acta Agrobotanica},

volume = {69},

number = {4},

publisher = {Polish Botanical Society},

abstract = {The article describes the current knowledge about the impact of nanoparticles on plant development with a particular emphasis on crop plants. Nanotechnology is an intensively developing field of science. This is due to the enormous hopes that have been placed on the achievements of nanotechnology in various areas of life. Increasingly, it has been noted that apart from the future benefits of nanotechnology in our everyday life, nanoparticles (NPs) may also have adverse effects that have not been sufficiently explored and understood. Most analyses to date have been focused on the influence of nanomaterials on the physiological processes primarily in animals, humans and bacteria. Although our knowledge about the influence of NPs on the development of plants is considerably smaller, the current views are presented below. Such knowledge is extremely important since NPs can enter the food chain, which may have an influence on human health. © The Author(s) 2016.},

note = {22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}