@article{2-s2.0-85123626788,

title = {The barley mutant happy under the sun 1 (hus1): An additional contribution to pale green crops},

author = { L. Rotasperti and L. Tadini and M. Chiara and C. Crosatti and D. Guerra and A. Tagliani and S. Forlani and I. Ezquer and D.S. Horner and P. Jahns and K. Gajek and A. García and R. Savin and L. Rossini and A. Tondelli and A. Janiak and P. Pesaresi},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123626788&doi=10.1016%2fj.envexpbot.2022.104795&partnerID=40&md5=4f093499bc9916285ba25ed8e6d639c7},

doi = {10.1016/j.envexpbot.2022.104795},

issn = {00988472},

year  = {2022},

date = {2022-01-01},

journal = {Environmental and Experimental Botany},

volume = {196},

publisher = {Elsevier B.V.},

abstract = {Truncated antenna size of photosystems and lower leaf chlorophyll content has been shown to increase photosynthetic efficiency and biomass accumulation in microalgae, cyanobacteria and higher plants grown under high-density cultivation conditions. Here, we have asked whether this strategy is also applicable to a major crop by characterizing the barley mutant happy under the sun 1 (hus1). The pale green phenotype of hus1 is due to a 50% reduction in the chlorophyll content of leaves, owing to a premature stop codon in the HvcpSRP43 gene for the 43-kDa chloroplast Signal Recognition Particle (cpSRP43). The HvcpSRP43 protein is responsible for the uploading of photosystem antenna proteins into the thylakoid membranes, and its truncation results in a smaller photosystem antenna size. Besides a detailed molecular and physiological characterization of the mutant grown under controlled greenhouse conditions, we show that the agronomic performance of hus1 plants, in terms of total biomass production and grain yield under standard field conditions, is comparable to that of control plants. The results are discussed in terms of the potential benefits of the hus1 phenotype, and of natural allelic variants of the HvcpSRP43 locus, with respect to productivity and mitigation of climate change. © 2022 Elsevier B.V.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85121336640,

title = {Whole exome sequencing-based identification of a novel gene involved in root hair development in barley (Hordeum vulgare l.)},

author = { K. Gajek and A. Janiak and U. Korotko and B. Chmielewska and M. Marzec and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121336640&doi=10.3390%2fijms222413411&partnerID=40&md5=0d73d429a527fcb38849ebab9bd1fe43},

doi = {10.3390/ijms222413411},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {24},

publisher = {MDPI},

abstract = {Root hairs play a crucial role in anchoring plants in soil, interaction with microorganisms and nutrient uptake from the rhizosphere. In contrast to Arabidopsis, there is a limited knowledge of root hair morphogenesis in monocots, including barley (Hordeum vulgare L.). We have isolated barley mutant rhp1.e with an abnormal root hair phenotype after chemical mutagenesis of spring cultivar ‘Sebastian’. The development of root hairs was initiated in the mutant but inhibited at the very early stage of tip growth. The length of root hairs reached only 3% of the length of parent cultivar. Using a whole exome sequencing (WES) approach, we identified G1674A mutation in the HORVU1Hr1G077230 gene, located on chromosome 1HL and encoding a cellulose synthase-like C1 protein (HvCSLC1) that might be involved in the xyloglucan (XyG) synthesis in root hairs. The identified mutation led to the retention of the second intron and premature termination of the HvCSLC1 protein. The mutation co-segregated with the abnormal root hair phenotype in the F2 progeny of rhp1.e mutant and its wild-type parent. Additionally, different substitutions in HORVU1Hr1G077230 were found in four other allelic mutants with the same root hair phenotype. Here, we discuss the putative role of HvCSLC1 protein in root hair tube elongation in barley. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85076702349,

title = {Drought stress and re-watering affect the abundance of TIP aquaporin transcripts in barley},

author = { M.M. Kurowska and K. Wiecha and K. Gajek and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076702349&doi=10.1371%2fjournal.pone.0226423&partnerID=40&md5=42b8a9dfdb4d4118b9ae9f4f914d17ac},

doi = {10.1371/journal.pone.0226423},

issn = {19326203},

year  = {2019},

date = {2019-01-01},

journal = {PLoS ONE},

volume = {14},

number = {12},

publisher = {Public Library of Science},

abstract = {Tonoplast Intrinsic Proteins (TIP) are plant aquaporins that are primarily localized in the tonoplast and play a role in the bidirectional flux of water and other substrates across a membrane. In barley, eleven members of the HvTIP gene subfamily have been identified. Here, we describe the transcription profile of the HvTIP genes in the leaves of barley seedlings being grown under optimal moisture conditions, drought stress and a re-watering phase. The applied drought stress caused a 55% decrease in the relative water content (RWC) in seedlings, while re-watering increased the RWC to 90% of the control. Our analysis showed that all HvTIP genes, except HvTIP3;2, HvTIP4;3 and HvTIP5.1, were expressed in leaves of ten-day-old barley seedlings under optimal water conditions with the transcripts of HvTIP2;3, HvTIP1;2 and HvTIP1;1 being the most abundant. We showed, for the first time in barley, a significant variation in the transcriptional activity between the analysed genes under drought stress. After drought treatment, five HvTIP genes, which are engaged in water transport, were down-regulated to varying degrees, while two, HvTIP3;1 and HvTIP4;1, were up-regulated. The HvTIP3;1 isoform, which is postulated as transporting hydrogen peroxide, expressed the highest increase of activity (ca. 5000x) under drought stress, thus indicating its importance in the response to this stress. Re-hydration caused the return of the expression of many genes to the level that was observed under optimal moisture conditions or, at least, a change in this direction Additionally, we examined the promotor regions of HvTIP and detected the presence of the cis-regulatory elements that are connected with the hormone and stress responses in all of the genes. Overall, our results suggest that 7 of 11 studied HvTIP (HvTIP1;1; HvTIP1;2; HvTIP2;1; HvTIP2;2; HvTIP2;3; HvTIP3;1; HvTIP4;1) have an important function during the adaptation of barley to drought stress conditions. We discuss the identified drought-responsive HvTIP in terms of their function in the adaptation of barley to this stress. Copyright: © 2019 Kurowska et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85071164859,

title = {Fragmentation of pooled PCR products for highly multiplexed TILLING},

author = { A. Tramontano and L. Jarc and J. Jankowicz-Cieslak and B.J. Hofinger and K. Gajek and M.E. Szurman-Zubrzycka and I. Szarejko and I. Ingelbrecht and B.J. Till},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071164859&doi=10.1534%2fg3.119.400301&partnerID=40&md5=3f8ee4568eb8403e7f6d7612d68f2bdb},

doi = {10.1534/g3.119.400301},

issn = {21601836},

year  = {2019},

date = {2019-01-01},

journal = {G3: Genes, Genomes, Genetics},

volume = {9},

number = {8},

pages = {2657-2666},

publisher = {Genetics Society of America},

abstract = {Improvements to massively parallel sequencing have allowed the routine recovery of natural and induced sequence variants. A broad range of biological disciplines have benefited from this, ranging from plant breeding to cancer research. The need for high sequence coverage to accurately recover single nucleotide variants and small insertions and deletions limits the applicability of whole genome approaches. This is especially true in organisms with a large genome size or for applications requiring the screening of thousands of individuals, such as the reverse-genetic technique known as TILLING. Using PCR to target and sequence chosen genomic regions provides an attractive alternative as the vast reduction in interrogated bases means that sample size can be dramatically increased through amplicon multiplexing and multidimensional sample pooling while maintaining suitable coverage for recovery of small mutations. Direct sequencing of PCR products is limited, however, due to limitations in read lengths of many next generation sequencers. In the present study we show the optimization and use of ultrasonication for the simultaneous fragmentation of multiplexed PCR amplicons for TILLING highly pooled samples. Sequencing performance was evaluated in a total of 32 pooled PCR products produced from 4096 chemically mutagenized Hordeum vulgare DNAs pooled in three dimensions. Evaluation of read coverage and base quality across amplicons suggests this approach is suitable for high-throughput TILLING and other applications employing highly pooled complex sampling schemes. Induced mutations previously identified in a traditional TILLING screen were recovered in this dataset further supporting the efficacy of the approach. Copyright © 2019 Tramontano et al.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85041351117,

title = {No time to waste: Transcriptome study reveals that drought tolerance in barley may be attributed to stressed-like expression patterns that exist before the occurrence of stress},

author = { A. Janiak and M. Kwaśniewski and M. Sowa and K. Gajek and K. Żmuda and J. Kościelniak and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041351117&doi=10.3389%2ffpls.2017.02212&partnerID=40&md5=29f8103607eab16eb24f63b99775273c},

doi = {10.3389/fpls.2017.02212},

issn = {1664462X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Plant Science},

volume = {8},

publisher = {Frontiers Media S.A.},

abstract = {Plant survival in adverse environmental conditions requires a substantial change in the metabolism, which is reflected by the extensive transcriptome rebuilding upon the occurrence of the stress. Therefore, transcriptomic studies offer an insight into the mechanisms of plant stress responses. Here, we present the results of global gene expression profiling of roots and leaves of two barley genotypes with contrasting ability to cope with drought stress. Our analysis suggests that drought tolerance results from a certain level of transcription of stress-influenced genes that is present even before the onset of drought. Genes that predispose the plant to better drought survival play a role in the regulatory network of gene expression, including several transcription factors, translation regulators and structural components of ribosomes. An important group of genes is involved in signaling mechanisms, with significant contribution of hormone signaling pathways and an interplay between ABA, auxin, ethylene and brassinosteroid homeostasis. Signal transduction in a drought tolerant genotype may be more efficient through the expression of genes required for environmental sensing that are active already during normal water availability and are related to actin filaments and LIMdomain proteins, which may function as osmotic biosensors. Better survival of drought may also be attributed to more effective processes of energy generation and more efficient chloroplasts biogenesis. Interestingly, our data suggest that several genes involved in a photosynthesis process are required for the establishment of effective drought response not only in leaves, but also in roots of barley. Thus, we propose a hypothesis that root plastids may turn into the anti-oxidative centers protecting root macromolecules from oxidative damage during drought stress. Specific genes and their potential role in building upa drought-tolerant barley phenotype is extensively discussedwith special emphasis on processes that take place in barley roots. When possible, the interconnections between particular factors are emphasized to drawa broader picture of the molecular mechanisms of drought tolerance in barley. © 2018 Janiak, Kwasniewski, Sowa, Gajek, Żmuda, Kościelniak.},

note = {35},

keywords = {},

pubstate = {published},

tppubtype = {article}

}