• dr Magdalena Pacwa-Płociniczak
Stanowisko: Adiunkt
Jednostka: Wydział Nauk Przyrodniczych
Adres: 40-032 Katowice, ul. Jagiellońska 28
Piętro: parter
Numer pokoju: A-40
Telefon: (32) 2009 442
E-mail: magdalena.pacwa-plociniczak@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 36894942800
Publikacje z bazy Scopus
2023
Noszczyńska, M.; Pacwa-Płociniczak, M.; Bondarczuk, K.; Piotrowska-Seget, Z.
The microbial removal of bisphenols in aquatic microcosms and associated alteration in bacterial community Journal Article
In: Environmental Science and Pollution Research, vol. 30, no. 36, pp. 85292-85304, 2023, ISSN: 09441344.
@article{2-s2.0-85163682040,
title = {The microbial removal of bisphenols in aquatic microcosms and associated alteration in bacterial community},
author = { M. Noszczyńska and M. Pacwa-Płociniczak and K. Bondarczuk and Z. Piotrowska-Seget},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85163682040&doi=10.1007%2fs11356-023-28305-2&partnerID=40&md5=db05224a4ae882bbfa3bf5f18d5f2125},
doi = {10.1007/s11356-023-28305-2},
issn = {09441344},
year = {2023},
date = {2023-01-01},
journal = {Environmental Science and Pollution Research},
volume = {30},
number = {36},
pages = {85292-85304},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {The concept of the study resulted from numerous concerns around bisphenol A (BPA) and bisphenol S (BPS) in aquatic environments. In this study, river water and sediment microcosms highly polluted with bisphenols and bioaugmented with two BPs-removing bacterial strains were constructed. The study aimed to determine the rate of high-concentrated BPA and BPS (BPs) removal from river water and sediment microniches, and the effect of water bioaugmentation with bacterial consortium on the removal rates of these pollutants. Moreover, the impact of introduced strains and exposure to BPs on the structural and functional composition of the autochthonous bacterial communities was elucidated. Our findings indicate that the removal activity of autochthonous bacteria was sufficient for effectively BPA elimination and reducing BPS content in the microcosms. The number of introduced bacterial cells decreased continuously until day 40, and on consecutive sampling days, no bioaugmented cells were detected. Sequencing analysis of the total 16S rRNA genes revealed that the community composition in bioaugmented microcosms amended with BPs differed significantly from those treated either with bacteria or BPs. A metagenomic analysis found an increase in the abundance of proteins responsible for xenobiotics removal in BPs-amended microcosms. This study provides new insights into the effects of bioaugmentation with a bacterial consortium on bacterial diversity and BPs removal in aquatic environments. © 2023, The Author(s).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The concept of the study resulted from numerous concerns around bisphenol A (BPA) and bisphenol S (BPS) in aquatic environments. In this study, river water and sediment microcosms highly polluted with bisphenols and bioaugmented with two BPs-removing bacterial strains were constructed. The study aimed to determine the rate of high-concentrated BPA and BPS (BPs) removal from river water and sediment microniches, and the effect of water bioaugmentation with bacterial consortium on the removal rates of these pollutants. Moreover, the impact of introduced strains and exposure to BPs on the structural and functional composition of the autochthonous bacterial communities was elucidated. Our findings indicate that the removal activity of autochthonous bacteria was sufficient for effectively BPA elimination and reducing BPS content in the microcosms. The number of introduced bacterial cells decreased continuously until day 40, and on consecutive sampling days, no bioaugmented cells were detected. Sequencing analysis of the total 16S rRNA genes revealed that the community composition in bioaugmented microcosms amended with BPs differed significantly from those treated either with bacteria or BPs. A metagenomic analysis found an increase in the abundance of proteins responsible for xenobiotics removal in BPs-amended microcosms. This study provides new insights into the effects of bioaugmentation with a bacterial consortium on bacterial diversity and BPs removal in aquatic environments. © 2023, The Author(s).
Pacwa-Płociniczak, M.; Byrski, A.; Chlebek, D.; Prach, M.; Płociniczak, T.
A deeper insight into the phytoremediation of soil polluted with petroleum hydrocarbons supported by the Enterobacter ludwigii ZCR5 strain Journal Article
In: Applied Soil Ecology, vol. 181, 2023, ISSN: 09291393, (4).
@article{2-s2.0-85137276108,
title = {A deeper insight into the phytoremediation of soil polluted with petroleum hydrocarbons supported by the Enterobacter ludwigii ZCR5 strain},
author = { M. Pacwa-Płociniczak and A. Byrski and D. Chlebek and M. Prach and T. Płociniczak},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85137276108&doi=10.1016%2fj.apsoil.2022.104651&partnerID=40&md5=5d2348787e46acc97c917687638552ee},
doi = {10.1016/j.apsoil.2022.104651},
issn = {09291393},
year = {2023},
date = {2023-01-01},
journal = {Applied Soil Ecology},
volume = {181},
publisher = {Elsevier B.V.},
abstract = {The Enterobacter ludwigii ZCR5 strain, isolated from the leaves of Zea mays growing in soil cocontaminated with hydrocarbons and heavy metals, was investigated under laboratory conditions for its plant growth-promoting traits, petroleum hydrocarbon (PH) degradation and biosurfactant production abilities and then used in bacterial-assisted phytoremediation. The whole genome of the ZCR5 was sequenced, and the presence of several genes encoding enzymes important for the enhancement of phytoremediation of soil polluted with PHs was detected. Next, the ZCR5 strain was introduced into polluted soil planted with Lolium perenne cv. Pinia. After inoculation, the ZCR5 strain was able to survive in polluted soil and colonized plant tissues. The highest efficacy of PHs removal (30.6 %) was observed for soil bioaugmented with living cells of the ZCR5, whereas in soil treated with bacterial necromass, it reached a value of 17.6 %. During the experimental period, the mechanisms enhancing the phytoremediation of PH-polluted soils were also tested in situ through the quantification of the expression of selected genes. The expression of genes encoding proteins involved in the biosurfactant production and promotion of plant growth by the ZCR5 strain was not detected either in the rhizosphere or in the endosphere of ryegrass. Among the genes involved in the degradation of PH, only CYP153 (in the rhizo- and endo-spheres) and nahAC (in the rhizosphere) were expressed. Despite the fact that we observed a higher efficiency of phytoremediation in the ZCR5-bioaugmented soil than in the controls, we were not able to indicate the bacterial mechanisms responsible for the observed effect of phytoremediation enhancement by the ZCR5 strain. The results obtained in our experiments indicate the necessity to study bacterial-plant interactions during assisted phytoremediation at the more detailed level. Thus the metatranscriptomic analysis of bacterial activity will be performed in the next step. © 2022 The Authors},
note = {4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The Enterobacter ludwigii ZCR5 strain, isolated from the leaves of Zea mays growing in soil cocontaminated with hydrocarbons and heavy metals, was investigated under laboratory conditions for its plant growth-promoting traits, petroleum hydrocarbon (PH) degradation and biosurfactant production abilities and then used in bacterial-assisted phytoremediation. The whole genome of the ZCR5 was sequenced, and the presence of several genes encoding enzymes important for the enhancement of phytoremediation of soil polluted with PHs was detected. Next, the ZCR5 strain was introduced into polluted soil planted with Lolium perenne cv. Pinia. After inoculation, the ZCR5 strain was able to survive in polluted soil and colonized plant tissues. The highest efficacy of PHs removal (30.6 %) was observed for soil bioaugmented with living cells of the ZCR5, whereas in soil treated with bacterial necromass, it reached a value of 17.6 %. During the experimental period, the mechanisms enhancing the phytoremediation of PH-polluted soils were also tested in situ through the quantification of the expression of selected genes. The expression of genes encoding proteins involved in the biosurfactant production and promotion of plant growth by the ZCR5 strain was not detected either in the rhizosphere or in the endosphere of ryegrass. Among the genes involved in the degradation of PH, only CYP153 (in the rhizo- and endo-spheres) and nahAC (in the rhizosphere) were expressed. Despite the fact that we observed a higher efficiency of phytoremediation in the ZCR5-bioaugmented soil than in the controls, we were not able to indicate the bacterial mechanisms responsible for the observed effect of phytoremediation enhancement by the ZCR5 strain. The results obtained in our experiments indicate the necessity to study bacterial-plant interactions during assisted phytoremediation at the more detailed level. Thus the metatranscriptomic analysis of bacterial activity will be performed in the next step. © 2022 The Authors
2022
Noszczyńska, M.; Pacwa-Płociniczak, M.; Bondarczuk, K.; Piotrowska-Seget, Z.
Avestia Publishing, 2022, ISSN: 23698128.
@proceedings{2-s2.0-85151329028,
title = {Bisphenol A And Bisphenol S Biodegradation In The River WaterSediment Microcosms And Their Impact On The Biodiversity Of Autochthonous Microbial Community},
author = { M. Noszczyńska and M. Pacwa-Płociniczak and K. Bondarczuk and Z. Piotrowska-Seget},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151329028&doi=10.11159%2ficepr22.105&partnerID=40&md5=c88dbba210330c9b885397e7e503741c},
doi = {10.11159/icepr22.105},
issn = {23698128},
year = {2022},
date = {2022-01-01},
journal = {Proceedings of the World Congress on New Technologies},
publisher = {Avestia Publishing},
abstract = {The concept of the study resulted from numerous concerns around bisphenol A (BPA) and bisphenol S (BPS) in aquatic environments. Previous works demonstrated that bioaugmentation with a pollutant-degrading consortium consisting of two or more microbial species was more effective in removing pollutants than with only a single strain. This effect relies on the fact that intermediates of one strain's catabolic pathway may be further utilized by other strains possessing the desired enzymes. Therefore, in this work, we used a BPA and BPS degrading bacterial consortium formed by Pseudomonas sp. BG12 and Acinetobacter sp. K1MN to amend river water-sediment microcosms polluted with these BPs. We aimed to (1) assess BPA and BPS biodegradation rates in created microcosms bioaugmented with the consortium during 70-day lasting experiment; (2) monitor the bacteria survival during the incubation period; (3) identify responses of the dominant bacterial community to bioaugmentation and BPs’ presence; (4) determine the core-indigenous microorganisms from water and sediment and their enzymes involved in BPS degradation. The effect of bioaugmentation of water-sediment microcosms with the consortium on BPA and BPS removal was assessed. Statistical analysis of obtained data showed significant differences (p<0.05) both between the time of sampling points and microcosms. BPA was removed from created microcosms within 40 days. The amount of BPS continuously decreased in all microcosms over the experimental time, but it was still detected in all microcosms at the 70th day of the experiment. Since the introduced consortium did not survive in tested treatments, BPs biodegradation was due to the activity of indigenous microflora. However, introduced bacterial strains can act as biofertilizers and stimulate changes in the composition and structure of an indigenous bacterial community. These changes were observed in our experiment. On day 35, the predominance of Thiobacillus, Dyella, and Hyphomicrobium were detected in created microcosms. The abundance of reads belonging to the Pseudomonas and Acinetobacter genera detected on day 35 was very low. Compared to day 35, different composition of bacterial communities in analyzed microcosms was observed on day 70. The predominance of the genus Thiobacillus, Rhodanobacter, Dyella, Hyphomicrobium, and Parvibaculum were observed. For Mesorhizobium, Achromobacter, and Mycobacterium, PICRUSt2 assigned metabolic pathways based on the calculated OTUs. The presence of protocatechuate and catechol degradation pathways were assigned for Achromobacter and Mycobacterium, while for Mesorhizobium only the catechol degradation pathway was identified. The relative number of OTUs assigned to these pathways was higher on day 35 than on day 70. In contrast, the relative number of OTUs assigned to the protocatechuate degradation pathway identified for Mycobacterium was higher at day 70 than 35. This study provides new insights into the effects of bioaugmentation with a bacterial consortium on bacterial diversity and BPs degradation in aquatic environments. © 2022, Avestia Publishing. All rights reserved.},
keywords = {},
pubstate = {published},
tppubtype = {proceedings}
}
The concept of the study resulted from numerous concerns around bisphenol A (BPA) and bisphenol S (BPS) in aquatic environments. Previous works demonstrated that bioaugmentation with a pollutant-degrading consortium consisting of two or more microbial species was more effective in removing pollutants than with only a single strain. This effect relies on the fact that intermediates of one strain's catabolic pathway may be further utilized by other strains possessing the desired enzymes. Therefore, in this work, we used a BPA and BPS degrading bacterial consortium formed by Pseudomonas sp. BG12 and Acinetobacter sp. K1MN to amend river water-sediment microcosms polluted with these BPs. We aimed to (1) assess BPA and BPS biodegradation rates in created microcosms bioaugmented with the consortium during 70-day lasting experiment; (2) monitor the bacteria survival during the incubation period; (3) identify responses of the dominant bacterial community to bioaugmentation and BPs’ presence; (4) determine the core-indigenous microorganisms from water and sediment and their enzymes involved in BPS degradation. The effect of bioaugmentation of water-sediment microcosms with the consortium on BPA and BPS removal was assessed. Statistical analysis of obtained data showed significant differences (p<0.05) both between the time of sampling points and microcosms. BPA was removed from created microcosms within 40 days. The amount of BPS continuously decreased in all microcosms over the experimental time, but it was still detected in all microcosms at the 70th day of the experiment. Since the introduced consortium did not survive in tested treatments, BPs biodegradation was due to the activity of indigenous microflora. However, introduced bacterial strains can act as biofertilizers and stimulate changes in the composition and structure of an indigenous bacterial community. These changes were observed in our experiment. On day 35, the predominance of Thiobacillus, Dyella, and Hyphomicrobium were detected in created microcosms. The abundance of reads belonging to the Pseudomonas and Acinetobacter genera detected on day 35 was very low. Compared to day 35, different composition of bacterial communities in analyzed microcosms was observed on day 70. The predominance of the genus Thiobacillus, Rhodanobacter, Dyella, Hyphomicrobium, and Parvibaculum were observed. For Mesorhizobium, Achromobacter, and Mycobacterium, PICRUSt2 assigned metabolic pathways based on the calculated OTUs. The presence of protocatechuate and catechol degradation pathways were assigned for Achromobacter and Mycobacterium, while for Mesorhizobium only the catechol degradation pathway was identified. The relative number of OTUs assigned to these pathways was higher on day 35 than on day 70. In contrast, the relative number of OTUs assigned to the protocatechuate degradation pathway identified for Mycobacterium was higher at day 70 than 35. This study provides new insights into the effects of bioaugmentation with a bacterial consortium on bacterial diversity and BPs degradation in aquatic environments. © 2022, Avestia Publishing. All rights reserved.
Chlebek, D.; Płociniczak, T.; Gobetti, S.; Kumor, A.; Hupert-Kocurek, K. T.; Pacwa-Płociniczak, M.
In: International Journal of Molecular Sciences, vol. 23, no. 1, 2022, ISSN: 16616596, (9).
@article{2-s2.0-85121668492,
title = {Analysis of the genome of the heavy metal resistant and hydrocarbon-degrading rhizospheric pseudomonas qingdaonensis zcr6 strain and assessment of its plant-growth-promoting traits},
author = { D. Chlebek and T. Płociniczak and S. Gobetti and A. Kumor and K.T. Hupert-Kocurek and M. Pacwa-Płociniczak},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121668492&doi=10.3390%2fijms23010214&partnerID=40&md5=db0b844c58764bf8192f1cb2aad87623},
doi = {10.3390/ijms23010214},
issn = {16616596},
year = {2022},
date = {2022-01-01},
journal = {International Journal of Molecular Sciences},
volume = {23},
number = {1},
publisher = {MDPI},
abstract = {The Pseudomonas qingdaonensis ZCR6 strain, isolated from the rhizosphere of Zea mays growing in soil co-contaminated with hydrocarbons and heavy metals, was investigated for its plant growth promotion, hydrocarbon degradation, and heavy metal resistance. In vitro bioassays confirmed all of the abovementioned properties. ZCR6 was able to produce indole acetic acid (IAA), siderophores, and ammonia, solubilized Ca3 (PO4 )2, and showed surface active properties and activity of cellulase and very high activity of 1-aminocyclopropane-1-carboxylic acid deaminase (297 nmol α-ketobutyrate mg−1 h−1 ). The strain degraded petroleum hydrocarbons (76.52% of the initial hydrocarbon content was degraded) and was resistant to Cd, Zn, and Cu (minimal inhibitory concentrations reached 5; 15; and 10 mM metal; respectively). The genome of the ZCR6 strain consisted of 5,507,067 bp, and a total of 5055 genes were annotated, of which 4943 were protein-coding sequences. Annotation revealed the presence of genes associated with nitrogen fixation, phosphate solubilization, sulfur metabolism, siderophore biosynthesis and uptake, synthesis of IAA, ethylene modulation, heavy metal resistance, exopolysaccharide biosynthesis, and organic compound degradation. Complete characteristics of the ZCR6 strain showed its potential multiway properties for enhancing the phytoremediation of co-contaminated soils. To our knowledge, this is the first analysis of the biotechnological potential of the species P. qingdaonensis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {9},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The Pseudomonas qingdaonensis ZCR6 strain, isolated from the rhizosphere of Zea mays growing in soil co-contaminated with hydrocarbons and heavy metals, was investigated for its plant growth promotion, hydrocarbon degradation, and heavy metal resistance. In vitro bioassays confirmed all of the abovementioned properties. ZCR6 was able to produce indole acetic acid (IAA), siderophores, and ammonia, solubilized Ca3 (PO4 )2, and showed surface active properties and activity of cellulase and very high activity of 1-aminocyclopropane-1-carboxylic acid deaminase (297 nmol α-ketobutyrate mg−1 h−1 ). The strain degraded petroleum hydrocarbons (76.52% of the initial hydrocarbon content was degraded) and was resistant to Cd, Zn, and Cu (minimal inhibitory concentrations reached 5; 15; and 10 mM metal; respectively). The genome of the ZCR6 strain consisted of 5,507,067 bp, and a total of 5055 genes were annotated, of which 4943 were protein-coding sequences. Annotation revealed the presence of genes associated with nitrogen fixation, phosphate solubilization, sulfur metabolism, siderophore biosynthesis and uptake, synthesis of IAA, ethylene modulation, heavy metal resistance, exopolysaccharide biosynthesis, and organic compound degradation. Complete characteristics of the ZCR6 strain showed its potential multiway properties for enhancing the phytoremediation of co-contaminated soils. To our knowledge, this is the first analysis of the biotechnological potential of the species P. qingdaonensis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
2020
Pacwa-Płociniczak, M.; Biniecka, Pa.; Bondarczuk, K.; Piotrowska-Seget, Z.
In: Frontiers in Microbiology, vol. 11, 2020, ISSN: 1664302X, (11).
@article{2-s2.0-85090876104,
title = {Metagenomic Functional Profiling Reveals Differences in Bacterial Composition and Function During Bioaugmentation of Aged Petroleum-Contaminated Soil},
author = { M. Pacwa-Płociniczak and Pa. Biniecka and K. Bondarczuk and Z. Piotrowska-Seget},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090876104&doi=10.3389%2ffmicb.2020.02106&partnerID=40&md5=6be3628cada1c6c91e4f223debdaf477},
doi = {10.3389/fmicb.2020.02106},
issn = {1664302X},
year = {2020},
date = {2020-01-01},
journal = {Frontiers in Microbiology},
volume = {11},
publisher = {Frontiers Media S.A.},
abstract = {Our objective was to study the bacterial community changes that determine enhanced removal of petroleum hydrocarbons from soils subjected to bioaugmentation with the hydrocarbon-degrading strains Rhodococcus erythropolis CD 130, CD 167, and their combination. To achieve this, a high-throughput sequencing of the 16S rRNA gene was performed. The changes in the bacterial community composition were most apparent the day after bacterial inoculation. These changes represented an increase in the percentage abundance of Rhodococcus and Pseudomonas genera. Surprisingly, members of the Rhodococcus genus were not present after day 91. At the end of the experiment, the bacterial communities from the CD 130, CD 167, and control soils had a similar structure. Nevertheless, the composition of the bacteria in the CD 130 + CD 167 soil was still distinct from the control. Metagenomic predictions from the 16S rRNA gene sequences showed that the introduction of bacteria had a significant influence on the predicted pathways (metabolism of xenobiotics; lipids; terpenoids; polyketides; and amino acids) on day one. On day 182, differences in the abundance of functional pathways were also detected in the CD 130 and CD 130 + CD 167 soils. Additionally, we observed that on day one, in all bioaugmented soils, the alkH gene was mainly contributed by the Rhodococcus and Mycobacterium genera, whereas in non-treated soil, this gene was contributed only by the Mycobacterium genus. Interestingly, from day 91, the Mycobacterium genus was the main contributor for the tested genes in all studied soils. Our results showed that hydrocarbon depletion from the analyzed soils resulted from the activity of the autochthonous bacteria. However, these changes in the composition and function of the indigenous bacterial community occurred under the influence of the introduced bacteria. © Copyright © 2020 Pacwa-Płociniczak, Biniecka, Bondarczuk and Piotrowska-Seget.},
note = {11},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Our objective was to study the bacterial community changes that determine enhanced removal of petroleum hydrocarbons from soils subjected to bioaugmentation with the hydrocarbon-degrading strains Rhodococcus erythropolis CD 130, CD 167, and their combination. To achieve this, a high-throughput sequencing of the 16S rRNA gene was performed. The changes in the bacterial community composition were most apparent the day after bacterial inoculation. These changes represented an increase in the percentage abundance of Rhodococcus and Pseudomonas genera. Surprisingly, members of the Rhodococcus genus were not present after day 91. At the end of the experiment, the bacterial communities from the CD 130, CD 167, and control soils had a similar structure. Nevertheless, the composition of the bacteria in the CD 130 + CD 167 soil was still distinct from the control. Metagenomic predictions from the 16S rRNA gene sequences showed that the introduction of bacteria had a significant influence on the predicted pathways (metabolism of xenobiotics; lipids; terpenoids; polyketides; and amino acids) on day one. On day 182, differences in the abundance of functional pathways were also detected in the CD 130 and CD 130 + CD 167 soils. Additionally, we observed that on day one, in all bioaugmented soils, the alkH gene was mainly contributed by the Rhodococcus and Mycobacterium genera, whereas in non-treated soil, this gene was contributed only by the Mycobacterium genus. Interestingly, from day 91, the Mycobacterium genus was the main contributor for the tested genes in all studied soils. Our results showed that hydrocarbon depletion from the analyzed soils resulted from the activity of the autochthonous bacteria. However, these changes in the composition and function of the indigenous bacterial community occurred under the influence of the introduced bacteria. © Copyright © 2020 Pacwa-Płociniczak, Biniecka, Bondarczuk and Piotrowska-Seget.
Ptaszek, N.; Pacwa-Płociniczak, M.; Noszczyńska, M.; Płociniczak, T.
Comparative study on multiway enhanced bio- And phytoremediation of aged petroleum-contaminated soil Journal Article
In: Agronomy, vol. 10, no. 7, 2020, ISSN: 20734395, (6).
@article{2-s2.0-85088455072,
title = {Comparative study on multiway enhanced bio- And phytoremediation of aged petroleum-contaminated soil},
author = { N. Ptaszek and M. Pacwa-Płociniczak and M. Noszczyńska and T. Płociniczak},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088455072&doi=10.3390%2fagronomy10070947&partnerID=40&md5=3233ddf61e84a0d289d4a54298bf1603},
doi = {10.3390/agronomy10070947},
issn = {20734395},
year = {2020},
date = {2020-01-01},
journal = {Agronomy},
volume = {10},
number = {7},
publisher = {MDPI AG},
abstract = {Bioremediation and phytoremediation of soil polluted with petroleum hydrocarbons (PHs) are an effective and eco-friendly alternative to physicochemical methods of soil decontamination. These techniques can be supported by the addition of effective strains and/or surface-active compounds. However, to obtain maximum efficacy of bioremediation, the interactions that occur between the microorganisms, enhancement factors and plants need to be studied. Our study aimed to investigate the removal of petroleum hydrocarbons from an aged and highly polluted soil (hydrocarbon content about 2.5%) using multiway enhanced bio- and phytoremediation. For this purpose, 10 enhanced experimental groups were compared to two untreated controls. Among the enhanced experimental groups, the bio- and phytoremediation processes were supported by the endophytic strain Rhodococcus erythropolis CDEL254. This bacterial strain has several plant growth-promoting traits and can degrade petroleum hydrocarbons and produce biosurfactants. Additionally, a rhamnolipid solution produced by Pseudomonas aeruginosa was used to support the total petroleum hydrocarbon loss from soil. After 112 days of incubation, the highest PH removal (31.1%) was observed in soil planted with ryegrass (Lolium perenne L. cv. Pearlgreen) treated with living cells of the CDEL254 strain and rhamnolipid solution. For non-planted experimental groups, the highest PH loss (26.1%) was detected for soil treated with heat-inactivated CDEL254 cells and a rhamnolipid solution. In general, the differences in the efficacy of the 10 experimental groups supported by plants, live/dead cells of the strain tested and rhamnolipid were not statistically significant. However, each of these groups was significantly more effective than the appropriate control groups. The PH loss in untreated (natural attenuation) and soils that underwent phytoremediation reached a value of 14.2% and 17.4%, respectively. Even though the CDEL254 strain colonized plant tissues and showed high survival in soil, its introduction did not significantly increase PH loss compared to systems treated with dead biomass. These results indicate that the development of effective biological techniques requires a customized approach to the polluted site and effective optimization of the methods used. © 2020 by the authors.},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Bioremediation and phytoremediation of soil polluted with petroleum hydrocarbons (PHs) are an effective and eco-friendly alternative to physicochemical methods of soil decontamination. These techniques can be supported by the addition of effective strains and/or surface-active compounds. However, to obtain maximum efficacy of bioremediation, the interactions that occur between the microorganisms, enhancement factors and plants need to be studied. Our study aimed to investigate the removal of petroleum hydrocarbons from an aged and highly polluted soil (hydrocarbon content about 2.5%) using multiway enhanced bio- and phytoremediation. For this purpose, 10 enhanced experimental groups were compared to two untreated controls. Among the enhanced experimental groups, the bio- and phytoremediation processes were supported by the endophytic strain Rhodococcus erythropolis CDEL254. This bacterial strain has several plant growth-promoting traits and can degrade petroleum hydrocarbons and produce biosurfactants. Additionally, a rhamnolipid solution produced by Pseudomonas aeruginosa was used to support the total petroleum hydrocarbon loss from soil. After 112 days of incubation, the highest PH removal (31.1%) was observed in soil planted with ryegrass (Lolium perenne L. cv. Pearlgreen) treated with living cells of the CDEL254 strain and rhamnolipid solution. For non-planted experimental groups, the highest PH loss (26.1%) was detected for soil treated with heat-inactivated CDEL254 cells and a rhamnolipid solution. In general, the differences in the efficacy of the 10 experimental groups supported by plants, live/dead cells of the strain tested and rhamnolipid were not statistically significant. However, each of these groups was significantly more effective than the appropriate control groups. The PH loss in untreated (natural attenuation) and soils that underwent phytoremediation reached a value of 14.2% and 17.4%, respectively. Even though the CDEL254 strain colonized plant tissues and showed high survival in soil, its introduction did not significantly increase PH loss compared to systems treated with dead biomass. These results indicate that the development of effective biological techniques requires a customized approach to the polluted site and effective optimization of the methods used. © 2020 by the authors.
Płociniczak, T.; Pacwa-Płociniczak, M.; Kwaśniewski, M.; Chwiałkowska, K.; Piotrowska-Seget, Z.
In: Ecotoxicology and Environmental Safety, vol. 194, 2020, ISSN: 01476513, (5).
@article{2-s2.0-85081025496,
title = {Response of rhizospheric and endophytic bacterial communities of white mustard (Sinapis alba) to bioaugmentation of soil with the Pseudomonas sp. H15 strain},
author = { T. Płociniczak and M. Pacwa-Płociniczak and M. Kwaśniewski and K. Chwiałkowska and Z. Piotrowska-Seget},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081025496&doi=10.1016%2fj.ecoenv.2020.110434&partnerID=40&md5=d8a193f0065138b2d4d3d54523d47078},
doi = {10.1016/j.ecoenv.2020.110434},
issn = {01476513},
year = {2020},
date = {2020-01-01},
journal = {Ecotoxicology and Environmental Safety},
volume = {194},
publisher = {Academic Press},
abstract = {A factor that may significantly increase the efficacy of phytoextraction is soil bioaugmentation with specific bacteria, which can alter the composition of rhizospheric and endophytic bacterial communities. The aim of this study was to compare the effect of soil treatment with living (bioaugmentation) and dead (control) cells of the plant growth-promoting metal-resistant endophytic strain Pseudomonas sp. H15 on the bacterial community composition in the rhizo- and endo-sphere of white mustard during enhanced phytoextraction. The bacterial communities in the rhizosphere were dominated (51.7–68.2%) by Proteobacteria, regardless of the soil treatment or sampling point. A temporary increase in the number of sequences belonging to Gammaproteobacteria (up to 37.3%) was only observed 24 h after the soil treatment with living Pseudomonas sp. H15 cells, whereas for the remaining samples, the relative abundance of this class did not exceed 7.1%. The relative abundance of Proteobacteria in the endosphere of the roots, stems, and leaves of white mustard was higher in the control than in bioaugmented plants. The most pronounced dominance of the Gammaproteobacteria sequences was observed in the stems and leaves of the control plants at the first sampling point, which strongly indicates the ability of the plants to rapidly uptake DNA from soil and translocate it to the aboveground parts of the plants. Additionally, the bioaugmentation of the soil caused a diverse shift in the bacterial communities in the rhizo- and endo-sphere of white mustard compared to control. The most distinct differences, which were dependent on the treatment, were observed in the endosphere of plants at the beginning of the experiment and decreased over time. These results indicate that the rhizo- and endo-biome of white mustard reacts to soil bioaugmentation and may influence the efficiency of bacterial-assisted phytoextraction. © 2020 Elsevier Inc.},
note = {5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A factor that may significantly increase the efficacy of phytoextraction is soil bioaugmentation with specific bacteria, which can alter the composition of rhizospheric and endophytic bacterial communities. The aim of this study was to compare the effect of soil treatment with living (bioaugmentation) and dead (control) cells of the plant growth-promoting metal-resistant endophytic strain Pseudomonas sp. H15 on the bacterial community composition in the rhizo- and endo-sphere of white mustard during enhanced phytoextraction. The bacterial communities in the rhizosphere were dominated (51.7–68.2%) by Proteobacteria, regardless of the soil treatment or sampling point. A temporary increase in the number of sequences belonging to Gammaproteobacteria (up to 37.3%) was only observed 24 h after the soil treatment with living Pseudomonas sp. H15 cells, whereas for the remaining samples, the relative abundance of this class did not exceed 7.1%. The relative abundance of Proteobacteria in the endosphere of the roots, stems, and leaves of white mustard was higher in the control than in bioaugmented plants. The most pronounced dominance of the Gammaproteobacteria sequences was observed in the stems and leaves of the control plants at the first sampling point, which strongly indicates the ability of the plants to rapidly uptake DNA from soil and translocate it to the aboveground parts of the plants. Additionally, the bioaugmentation of the soil caused a diverse shift in the bacterial communities in the rhizo- and endo-sphere of white mustard compared to control. The most distinct differences, which were dependent on the treatment, were observed in the endosphere of plants at the beginning of the experiment and decreased over time. These results indicate that the rhizo- and endo-biome of white mustard reacts to soil bioaugmentation and may influence the efficiency of bacterial-assisted phytoextraction. © 2020 Elsevier Inc.
2019
Płociniczak, T.; Chodór, M.; Pacwa-Płociniczak, M.; Piotrowska-Seget, Z.
Metal-tolerant endophytic bacteria associated with Silene vulgaris support the Cd and Zn phytoextraction in non-host plants Journal Article
In: Chemosphere, vol. 219, pp. 250-260, 2019, ISSN: 00456535, (37).
@article{2-s2.0-85058032943,
title = {Metal-tolerant endophytic bacteria associated with Silene vulgaris support the Cd and Zn phytoextraction in non-host plants},
author = { T. Płociniczak and M. Chodór and M. Pacwa-Płociniczak and Z. Piotrowska-Seget},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058032943&doi=10.1016%2fj.chemosphere.2018.12.018&partnerID=40&md5=22d7bcbc236a88cd13ff51a612ec9e27},
doi = {10.1016/j.chemosphere.2018.12.018},
issn = {00456535},
year = {2019},
date = {2019-01-01},
journal = {Chemosphere},
volume = {219},
pages = {250-260},
publisher = {Elsevier Ltd},
abstract = {The aim of this study was to isolate and characterise metal-resistant endophytic bacteria from the tissues of Silene vulgaris collected within the vicinity of non-ferrous steelworks in Katowice, Upper Silesia, Southern Poland. Twenty-four strains of metal-resistant endophytic bacteria that belong to 15 genera were isolated from the stems and leaves of Silene vulgaris. Most of these strains showed multiple plant growth-promoting capabilities. The most promising strains, Proteus vulgaris H7, Pseudomonas sp. H15, and Pseudomonas helmanticensis H16, were used in a pot experiment, and their impact on the biomass of white mustard and Zn and Cd accumulation was examined. Soil inoculation with the tested strains resulted in a higher fresh biomass of shoots, which increased by 74.5% (Proteus vulgaris H7), 121.7% (Pseudomonas sp. H15), and 142.2% (P. helmanticensis H16) compared to the control plants. The highest phytoextraction enhancement was caused by P. helmanticensis H16, which increased Zn and Cd accumulation in the shoot tissues by 43.8% and 112.6%, respectively. All of the tested strains were detected in the soil at the last sampling points, but only Proteus vulgaris H7 and Pseudomonas sp. H15 were capable of temporary colonisation of the roots of white mustard. None of the inoculants were found in the stems and leaves of the plants during the experimental period. The plant growth-promoting features of the isolates combined with their resistance to heavy metals and high survival in soil after inoculation make these strains good candidates for the promotion of plant growth and increased phytoremediation efficiency. © 2018 Elsevier Ltd},
note = {37},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of this study was to isolate and characterise metal-resistant endophytic bacteria from the tissues of Silene vulgaris collected within the vicinity of non-ferrous steelworks in Katowice, Upper Silesia, Southern Poland. Twenty-four strains of metal-resistant endophytic bacteria that belong to 15 genera were isolated from the stems and leaves of Silene vulgaris. Most of these strains showed multiple plant growth-promoting capabilities. The most promising strains, Proteus vulgaris H7, Pseudomonas sp. H15, and Pseudomonas helmanticensis H16, were used in a pot experiment, and their impact on the biomass of white mustard and Zn and Cd accumulation was examined. Soil inoculation with the tested strains resulted in a higher fresh biomass of shoots, which increased by 74.5% (Proteus vulgaris H7), 121.7% (Pseudomonas sp. H15), and 142.2% (P. helmanticensis H16) compared to the control plants. The highest phytoextraction enhancement was caused by P. helmanticensis H16, which increased Zn and Cd accumulation in the shoot tissues by 43.8% and 112.6%, respectively. All of the tested strains were detected in the soil at the last sampling points, but only Proteus vulgaris H7 and Pseudomonas sp. H15 were capable of temporary colonisation of the roots of white mustard. None of the inoculants were found in the stems and leaves of the plants during the experimental period. The plant growth-promoting features of the isolates combined with their resistance to heavy metals and high survival in soil after inoculation make these strains good candidates for the promotion of plant growth and increased phytoremediation efficiency. © 2018 Elsevier Ltd
Pacwa-Płociniczak, M.; Czapla, J.; Płociniczak, T.; Piotrowska-Seget, Z.
The effect of bioaugmentation of petroleum-contaminated soil with Rhodococcus erythropolis strains on removal of petroleum from soil Journal Article
In: Ecotoxicology and Environmental Safety, vol. 169, pp. 615-622, 2019, ISSN: 01476513, (33).
@article{2-s2.0-85057054295,
title = {The effect of bioaugmentation of petroleum-contaminated soil with Rhodococcus erythropolis strains on removal of petroleum from soil},
author = { M. Pacwa-Płociniczak and J. Czapla and T. Płociniczak and Z. Piotrowska-Seget},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057054295&doi=10.1016%2fj.ecoenv.2018.11.081&partnerID=40&md5=216b4d448e42c30bd416107f744858a2},
doi = {10.1016/j.ecoenv.2018.11.081},
issn = {01476513},
year = {2019},
date = {2019-01-01},
journal = {Ecotoxicology and Environmental Safety},
volume = {169},
pages = {615-622},
publisher = {Academic Press},
abstract = {The aim of the study was to assess the impact of inoculation of petroleum-contaminated soil with the hydrocarbon-degrading bacterial strains Rhodococcus erythropolis CD 130 and CD 167 or their consortium on the removal of hydrocarbons from the soil. Additionally, changes in the activity and structure of soil autochthonous bacterial communities were studied. At the end of the experiment, the fastest hydrocarbon removal was seen in the soil treated with the CD 167 strain (38.40%) and was statistically higher compared to the removal of total petroleum hydrocarbons (TPH) observed in soils inoculated with strain CD 130 (29.8%) or bacterial consortium CD 130 + CD 167 (29.72%). The rifampicin-resistant CD 130 and CD 167 strains, introduced as single strains or a consortium, survived in the soil for 42 days. The introduction of gram-positive strains of R. erythropolis primarily caused an increase in the biomass of branched phospholipid fatty acids (PLFAs), characteristic for gram-positive bacteria. Nevertheless, changes in the concentrations of gram-positive and gram-negative PLFA markers were periodic, and at the end of the experiment, significant changes were observed only in the case of the soil bioaugmented with the CD 167 strain. After the bioaugmentation, higher values of substrate-induced respiration (SIR) were observed in all the inoculated soils compared to the non-inoculated control. Nonetheless, after 91 days of incubation, a significant decrease in soil respiration was observed in the soil treated with single CD 130 or CD 167 strains or with their consortium. The number of transcripts of the CYP153 gene obtained on days 91 and 182 reflected the results of the hydrocarbon loss. The level of expression of the alkH gene in experimental soil was estimated and found to be higher than the level of expression of the CYP153 gene but did not coincide with the loss of hydrocarbons. The introduction of strains CD 130, CD 167, or CD 130 + CD 167 caused temporary changes in the composition of the soil autochthonous bacterial community, but it seems that these changes were needed for the enhanced removal of hydrocarbons from this soil. © 2018 Elsevier Inc.},
note = {33},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of the study was to assess the impact of inoculation of petroleum-contaminated soil with the hydrocarbon-degrading bacterial strains Rhodococcus erythropolis CD 130 and CD 167 or their consortium on the removal of hydrocarbons from the soil. Additionally, changes in the activity and structure of soil autochthonous bacterial communities were studied. At the end of the experiment, the fastest hydrocarbon removal was seen in the soil treated with the CD 167 strain (38.40%) and was statistically higher compared to the removal of total petroleum hydrocarbons (TPH) observed in soils inoculated with strain CD 130 (29.8%) or bacterial consortium CD 130 + CD 167 (29.72%). The rifampicin-resistant CD 130 and CD 167 strains, introduced as single strains or a consortium, survived in the soil for 42 days. The introduction of gram-positive strains of R. erythropolis primarily caused an increase in the biomass of branched phospholipid fatty acids (PLFAs), characteristic for gram-positive bacteria. Nevertheless, changes in the concentrations of gram-positive and gram-negative PLFA markers were periodic, and at the end of the experiment, significant changes were observed only in the case of the soil bioaugmented with the CD 167 strain. After the bioaugmentation, higher values of substrate-induced respiration (SIR) were observed in all the inoculated soils compared to the non-inoculated control. Nonetheless, after 91 days of incubation, a significant decrease in soil respiration was observed in the soil treated with single CD 130 or CD 167 strains or with their consortium. The number of transcripts of the CYP153 gene obtained on days 91 and 182 reflected the results of the hydrocarbon loss. The level of expression of the alkH gene in experimental soil was estimated and found to be higher than the level of expression of the CYP153 gene but did not coincide with the loss of hydrocarbons. The introduction of strains CD 130, CD 167, or CD 130 + CD 167 caused temporary changes in the composition of the soil autochthonous bacterial community, but it seems that these changes were needed for the enhanced removal of hydrocarbons from this soil. © 2018 Elsevier Inc.
2018
Pacwa-Płociniczak, M.; Płociniczak, T.; Yu, D.; Kurola, J. M.; Sinkkonen, A.; Piotrowska-Seget, Z.; Romantschuk, M.
Effect of Silene vulgaris and Heavy Metal Pollution on Soil Microbial Diversity in Long-Term Contaminated Soil Journal Article
In: Water, Air, and Soil Pollution, vol. 229, no. 1, 2018, ISSN: 00496979, (34).
@article{2-s2.0-85040236045,
title = {Effect of Silene vulgaris and Heavy Metal Pollution on Soil Microbial Diversity in Long-Term Contaminated Soil},
author = { M. Pacwa-Płociniczak and T. Płociniczak and D. Yu and J.M. Kurola and A. Sinkkonen and Z. Piotrowska-Seget and M. Romantschuk},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040236045&doi=10.1007%2fs11270-017-3655-3&partnerID=40&md5=c3554399dc403e2252677ae08796fad4},
doi = {10.1007/s11270-017-3655-3},
issn = {00496979},
year = {2018},
date = {2018-01-01},
journal = {Water, Air, and Soil Pollution},
volume = {229},
number = {1},
publisher = {Springer International Publishing},
abstract = {In this study, we analysed the impact of heavy metals and plant rhizodeposition on the structure of indigenous microbial communities in rhizosphere and bulk soil that had been exposed to heavy metals for more than 150 years. Samples of the rhizosphere of Silene vulgaris and non-rhizosphere soils 250 and 450 m from the source of emission that had different metal concentrations were collected for analyses. The results showed that soils were collected 250 m from the smelter had a higher number of Cd-resistant CFU compared with the samples that were collected from 450 m, but no significant differences were observed in the number of total and oligotrophic CFU or the equivalent cell numbers between rhizosphere and non-rhizosphere soils that were taken 250 and 450 m from the emitter. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis of the denaturing gradient gel electrophoresis (DGGE) profiles, as well as a cluster analysis that was generated on the phospholipid fatty acid (PLFA) profiles, showed that the bacterial community structure of rhizosphere soils depended more on the plant than on the distance and metal concentrations. The sequencing of the 16S rDNA fragments that were excised from the DGGE gel revealed representatives of the phyla Bacteroidetes, Acidobacteria, Gemmatimonadetes, Actinobacteria and Betaproteobacteria in the analysed soil with a predominance of the first three groups. The obtained results demonstrated that the presence of S. vulgaris did not affect the number of CFUs, except for those of Cd-resistant bacteria. However, the presence of S. vulgaris altered the soil bacterial community structure, regardless of the sampling site, which supported the thesis that plants have a higher impact on soil microbial community than metal contamination. © 2017, The Author(s).},
note = {34},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this study, we analysed the impact of heavy metals and plant rhizodeposition on the structure of indigenous microbial communities in rhizosphere and bulk soil that had been exposed to heavy metals for more than 150 years. Samples of the rhizosphere of Silene vulgaris and non-rhizosphere soils 250 and 450 m from the source of emission that had different metal concentrations were collected for analyses. The results showed that soils were collected 250 m from the smelter had a higher number of Cd-resistant CFU compared with the samples that were collected from 450 m, but no significant differences were observed in the number of total and oligotrophic CFU or the equivalent cell numbers between rhizosphere and non-rhizosphere soils that were taken 250 and 450 m from the emitter. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis of the denaturing gradient gel electrophoresis (DGGE) profiles, as well as a cluster analysis that was generated on the phospholipid fatty acid (PLFA) profiles, showed that the bacterial community structure of rhizosphere soils depended more on the plant than on the distance and metal concentrations. The sequencing of the 16S rDNA fragments that were excised from the DGGE gel revealed representatives of the phyla Bacteroidetes, Acidobacteria, Gemmatimonadetes, Actinobacteria and Betaproteobacteria in the analysed soil with a predominance of the first three groups. The obtained results demonstrated that the presence of S. vulgaris did not affect the number of CFUs, except for those of Cd-resistant bacteria. However, the presence of S. vulgaris altered the soil bacterial community structure, regardless of the sampling site, which supported the thesis that plants have a higher impact on soil microbial community than metal contamination. © 2017, The Author(s).
2017
Płociniczak, T.; Fic, E.; Pacwa-Płociniczak, M.; Pawlik, M.; Piotrowska-Seget, Z.
Improvement of phytoremediation of an aged petroleum hydrocarbon-contaminated soil by Rhodococcus erythropolis CD 106 strain Journal Article
In: International Journal of Phytoremediation, vol. 19, no. 7, pp. 614-620, 2017, ISSN: 15226514, (20).
@article{2-s2.0-85019267226,
title = {Improvement of phytoremediation of an aged petroleum hydrocarbon-contaminated soil by Rhodococcus erythropolis CD 106 strain},
author = { T. Płociniczak and E. Fic and M. Pacwa-Płociniczak and M. Pawlik and Z. Piotrowska-Seget},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019267226&doi=10.1080%2f15226514.2016.1278420&partnerID=40&md5=2ccee0f9bdf71f304543948e2d5fd67f},
doi = {10.1080/15226514.2016.1278420},
issn = {15226514},
year = {2017},
date = {2017-01-01},
journal = {International Journal of Phytoremediation},
volume = {19},
number = {7},
pages = {614-620},
publisher = {Taylor and Francis Inc.},
abstract = {The aim of this study was to assess the impact of soil inoculation with the Rhodococcus erythropolis CD 106 strain on the effectiveness of the phytoremediation of an aged hydrocarbon-contaminated [approx. 1% total petroleum hydrocarbon (TPH)] soil using ryegrass (Lolium perenne). The introduction of CD 106 into the soil significantly increased the biomass of ryegrass and the removal of hydrocarbons in planted soil. The fresh weight of the shoots and roots of plants inoculated with CD 106 increased by 49% and 30%, respectively. After 210 days of the experiment, the concentration of TPH was reduced by 31.2%, whereas in the planted, non-inoculated soil, it was reduced by 16.8%. By contrast, the concentration of petroleum hydrocarbon decreased by 18.7% in non-planted soil bioaugmented with the CD 106 strain. The rifampicin-resistant CD 106 strain survived after inoculation into soil and was detected in the soil during the entire experimental period, but the number of CD 106 cells decreased constantly during the enhanced phytoremediation and bioaugmentation experiments. The plant growth-promoting and hydrocarbon-degrading properties of CD 106, which are connected with its long-term survival and limited impact on autochthonous microflora, make this strain a good candidate for improving the phytoremediation efficiency of soil contaminated with hydrocarbons. © 2017 Taylor & Francis Group, LLC.},
note = {20},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of this study was to assess the impact of soil inoculation with the Rhodococcus erythropolis CD 106 strain on the effectiveness of the phytoremediation of an aged hydrocarbon-contaminated [approx. 1% total petroleum hydrocarbon (TPH)] soil using ryegrass (Lolium perenne). The introduction of CD 106 into the soil significantly increased the biomass of ryegrass and the removal of hydrocarbons in planted soil. The fresh weight of the shoots and roots of plants inoculated with CD 106 increased by 49% and 30%, respectively. After 210 days of the experiment, the concentration of TPH was reduced by 31.2%, whereas in the planted, non-inoculated soil, it was reduced by 16.8%. By contrast, the concentration of petroleum hydrocarbon decreased by 18.7% in non-planted soil bioaugmented with the CD 106 strain. The rifampicin-resistant CD 106 strain survived after inoculation into soil and was detected in the soil during the entire experimental period, but the number of CD 106 cells decreased constantly during the enhanced phytoremediation and bioaugmentation experiments. The plant growth-promoting and hydrocarbon-degrading properties of CD 106, which are connected with its long-term survival and limited impact on autochthonous microflora, make this strain a good candidate for improving the phytoremediation efficiency of soil contaminated with hydrocarbons. © 2017 Taylor & Francis Group, LLC.
2016
Pacwa-Płociniczak, M.; Płaza, G. A.; Piotrowska-Seget, Z.
Monitoring the changes in a bacterial community in petroleum-polluted soil bioaugmented with hydrocarbon-degrading strains Journal Article
In: Applied Soil Ecology, vol. 105, pp. 76-85, 2016, ISSN: 09291393, (31).
@article{2-s2.0-84963591516,
title = {Monitoring the changes in a bacterial community in petroleum-polluted soil bioaugmented with hydrocarbon-degrading strains},
author = { M. Pacwa-Płociniczak and G.A. Płaza and Z. Piotrowska-Seget},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84963591516&doi=10.1016%2fj.apsoil.2016.04.005&partnerID=40&md5=08f6293adbad0098aea02d5034247bc4},
doi = {10.1016/j.apsoil.2016.04.005},
issn = {09291393},
year = {2016},
date = {2016-01-01},
journal = {Applied Soil Ecology},
volume = {105},
pages = {76-85},
publisher = {Elsevier B.V.},
abstract = {Bioaugmentation is a strategy used to enhance degradation of petroleum compounds in contaminated soils, however little is known about the interactions between introduced bacteria and autochthonous microflora. Therefore the objective of the study was to assess any changes in the structure and metabolic activity of the soil bacterial communities as a result of the introduction of Bacillus subtilis T'-1 or Pseudomonas sp. P-1, as well their consortium, into petroleum-contaminated soil. The bioaugmentation experiment was carried out under laboratory conditions using soil taken from an industrial area located around a refinery in Czechowice-Dziedzice. After the bioaugmentation process, a significant (P < 0.05) decrease in the TPH content was reported in all inoculated soils. Inoculation of the soil with the bacterial consortium resulted in a three times greater removal of TPH compared to soils inoculated with single strains. It has been reported that all of the strains had an ability to survive in the environment during the experimental period. The introduction of bacterial strains led to increase in the number of 16S rRNA gene copies in soil on 1 and 7 days of the experiment as well as alkB gene copies during 91 days of the study compared to the non-treated soil. Analysis of the 16S rRNA and alkB genes-based DGGE fingerprints showed that introduced bacteria changed the genetic diversity of the total bacterial communities as well as the communities that have the genes involved in the degradation of hydrocarbons. Analysis of the PLFA profiles showed that the bacterial strains caused short-term changes in the amounts of fatty acids characteristic for Gram-positive and Gram-negative bacteria. The CLPPs indicated differences in soil metabolic activity between the inoculated and non-inoculated soils after the bioaugmentation process. © 2016 Elsevier B.V.},
note = {31},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Bioaugmentation is a strategy used to enhance degradation of petroleum compounds in contaminated soils, however little is known about the interactions between introduced bacteria and autochthonous microflora. Therefore the objective of the study was to assess any changes in the structure and metabolic activity of the soil bacterial communities as a result of the introduction of Bacillus subtilis T'-1 or Pseudomonas sp. P-1, as well their consortium, into petroleum-contaminated soil. The bioaugmentation experiment was carried out under laboratory conditions using soil taken from an industrial area located around a refinery in Czechowice-Dziedzice. After the bioaugmentation process, a significant (P < 0.05) decrease in the TPH content was reported in all inoculated soils. Inoculation of the soil with the bacterial consortium resulted in a three times greater removal of TPH compared to soils inoculated with single strains. It has been reported that all of the strains had an ability to survive in the environment during the experimental period. The introduction of bacterial strains led to increase in the number of 16S rRNA gene copies in soil on 1 and 7 days of the experiment as well as alkB gene copies during 91 days of the study compared to the non-treated soil. Analysis of the 16S rRNA and alkB genes-based DGGE fingerprints showed that introduced bacteria changed the genetic diversity of the total bacterial communities as well as the communities that have the genes involved in the degradation of hydrocarbons. Analysis of the PLFA profiles showed that the bacterial strains caused short-term changes in the amounts of fatty acids characteristic for Gram-positive and Gram-negative bacteria. The CLPPs indicated differences in soil metabolic activity between the inoculated and non-inoculated soils after the bioaugmentation process. © 2016 Elsevier B.V.
Pacwa-Płociniczak, M.; Płociniczak, T.; Iwan, J.; Zarska, M.; Chorazewski, M.; Dzida, M.; Piotrowska-Seget, Z.
Isolation of hydrocarbon-degrading and biosurfactant-producing bacteria and assessment their plant growth-promoting traits Journal Article
In: Journal of Environmental Management, vol. 168, pp. 175-184, 2016, ISSN: 03014797, (44).
@article{2-s2.0-84949845363,
title = {Isolation of hydrocarbon-degrading and biosurfactant-producing bacteria and assessment their plant growth-promoting traits},
author = { M. Pacwa-Płociniczak and T. Płociniczak and J. Iwan and M. Zarska and M. Chorazewski and M. Dzida and Z. Piotrowska-Seget},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84949845363&doi=10.1016%2fj.jenvman.2015.11.058&partnerID=40&md5=a87f69d8c6d64170f34a59d83a5c2740},
doi = {10.1016/j.jenvman.2015.11.058},
issn = {03014797},
year = {2016},
date = {2016-01-01},
journal = {Journal of Environmental Management},
volume = {168},
pages = {175-184},
publisher = {Academic Press},
abstract = {Forty-two hydrocarbon-degrading bacterial strains were isolated from the soil heavily contaminated with petroleum hydrocarbons. Forty-one strains were identified based on their whole-cell fatty acid profiles using the MIDI-MIS method. Thirty-three of them belong to species Rhodococcus erythropolis, while the others to the genera Rahnella (4), Serratia (3) and Proteus (1). Isolates were screened for their ability to produce biosurfactants/bioemulsifiers. For all of them the activity of several mechanisms characteristic for plant growth-promoting bacteria was also determined. In order to investigate surface active and emulsifying abilities of isolates following methods: oil-spreading, blood agar, methylene blue agar and determination of emulsification index, were used. Among studied bacteria 12 strains (CD 112; CD 126; CD 131; CD 132; CD 135; CD 147; CD 154; CD 155; CD 158; CD 161; CD 166 and CD 167) have been chosen as promising candidates for the production of biosurfactants and/or bioemulsifiers. Among them 2 strains (R. erythropolis CD 126 and Rahnella aquatilis CD 132) had the highest potential to be used in the bioaugmentation of PH-contaminated soil. Moreover, 15 of tested strains (CD 105; CD 106; CD 108; CD 111; CD 116; CD 120; CD 124; CD 125; CD 130; CD 132; CD 134; CD 154; CD 156; CD 161 and CD 170) showed the activity of four mechanisms (ACC deaminase activity; IAA and siderophore production; phosphate solubilization) considered to be characteristic for plant growth-promoting bacteria. Two of them (R. erythropolis CD 106 and R. erythropolis CD 111) showed the highest activity of above-mentioned mechanisms and thus are considered as promising agents in microbe assisted phytoremediation. © 2015 Elsevier Ltd.},
note = {44},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Forty-two hydrocarbon-degrading bacterial strains were isolated from the soil heavily contaminated with petroleum hydrocarbons. Forty-one strains were identified based on their whole-cell fatty acid profiles using the MIDI-MIS method. Thirty-three of them belong to species Rhodococcus erythropolis, while the others to the genera Rahnella (4), Serratia (3) and Proteus (1). Isolates were screened for their ability to produce biosurfactants/bioemulsifiers. For all of them the activity of several mechanisms characteristic for plant growth-promoting bacteria was also determined. In order to investigate surface active and emulsifying abilities of isolates following methods: oil-spreading, blood agar, methylene blue agar and determination of emulsification index, were used. Among studied bacteria 12 strains (CD 112; CD 126; CD 131; CD 132; CD 135; CD 147; CD 154; CD 155; CD 158; CD 161; CD 166 and CD 167) have been chosen as promising candidates for the production of biosurfactants and/or bioemulsifiers. Among them 2 strains (R. erythropolis CD 126 and Rahnella aquatilis CD 132) had the highest potential to be used in the bioaugmentation of PH-contaminated soil. Moreover, 15 of tested strains (CD 105; CD 106; CD 108; CD 111; CD 116; CD 120; CD 124; CD 125; CD 130; CD 132; CD 134; CD 154; CD 156; CD 161 and CD 170) showed the activity of four mechanisms (ACC deaminase activity; IAA and siderophore production; phosphate solubilization) considered to be characteristic for plant growth-promoting bacteria. Two of them (R. erythropolis CD 106 and R. erythropolis CD 111) showed the highest activity of above-mentioned mechanisms and thus are considered as promising agents in microbe assisted phytoremediation. © 2015 Elsevier Ltd.
2015
Grażyna, A. P.; Pacwa-Płociniczak, M.; Piotrowska-Seget, Z.; Brigmon, R.; Król, E.
Characterization of bacillus strains producing biosurfactants Book Chapter
In: pp. 173-184, Springer India, 2015, ISBN: 9788132220565; 9788132220558, (3).
@inbook{2-s2.0-84943251266,
title = {Characterization of bacillus strains producing biosurfactants},
author = { A.P. Grażyna and M. Pacwa-Płociniczak and Z. Piotrowska-Seget and R. Brigmon and E. Król},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84943251266&doi=10.1007%2f978-81-322-2056-5_10&partnerID=40&md5=6e8fd438164a6d8f15d32d0a332338fd},
doi = {10.1007/978-81-322-2056-5_10},
isbn = {9788132220565; 9788132220558},
year = {2015},
date = {2015-01-01},
journal = {Environmental Sustainability: Role of Green Technologies},
pages = {173-184},
publisher = {Springer India},
abstract = {Genus Bacillus includes species of industrial, biotechnological, and environmental interest, as well as clinically important strains. In terms of metabolic properties, they present a diverse group, as they can degrade various substrates and produce many molecules, including lipopeptide (LP) biosurfactants. Due to a high interest in biosurfactants for application in different fields, the molecular mechanisms of regulation of the expression of the operons responsible for LPs have been intensively studied. Additionally, many assays have been created to evaluate the use of cost-effective renewable agro-industrial substrates for production. The purpose of the chapter is to provide a comprehensive overview of the results of our studies on identification, characterization, and assessment ability of three Bacillus strains to produce biosurfactants and detection of genes encoding enzymes involved in biosurfactant synthesis. Moreover, the use of alternative substrates to decrease the cost of LP biosurfactant production and some aspects of application of Bacillus spp. as biocontrol agents are discussed. © Springer India 2015.},
note = {3},
keywords = {},
pubstate = {published},
tppubtype = {inbook}
}
Genus Bacillus includes species of industrial, biotechnological, and environmental interest, as well as clinically important strains. In terms of metabolic properties, they present a diverse group, as they can degrade various substrates and produce many molecules, including lipopeptide (LP) biosurfactants. Due to a high interest in biosurfactants for application in different fields, the molecular mechanisms of regulation of the expression of the operons responsible for LPs have been intensively studied. Additionally, many assays have been created to evaluate the use of cost-effective renewable agro-industrial substrates for production. The purpose of the chapter is to provide a comprehensive overview of the results of our studies on identification, characterization, and assessment ability of three Bacillus strains to produce biosurfactants and detection of genes encoding enzymes involved in biosurfactant synthesis. Moreover, the use of alternative substrates to decrease the cost of LP biosurfactant production and some aspects of application of Bacillus spp. as biocontrol agents are discussed. © Springer India 2015.
2014
Pacwa-Płociniczak, M.; Płaza, G. A.; Poliwoda, A.; Piotrowska-Seget, Z.
In: Environmental Science and Pollution Research, vol. 21, no. 15, pp. 9385-9395, 2014, ISSN: 09441344, (68).
@article{2-s2.0-84905679951,
title = {Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil},
author = { M. Pacwa-Płociniczak and G.A. Płaza and A. Poliwoda and Z. Piotrowska-Seget},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84905679951&doi=10.1007%2fs11356-014-2872-1&partnerID=40&md5=ec6d3cfa413b19cb9a07ef1ebb31b50d},
doi = {10.1007/s11356-014-2872-1},
issn = {09441344},
year = {2014},
date = {2014-01-01},
journal = {Environmental Science and Pollution Research},
volume = {21},
number = {15},
pages = {9385-9395},
publisher = {Springer Verlag},
abstract = {The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27; 39; 27 and 13 % of hydrocarbons added to culture medium were degraded; respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils. © 2014 The Author(s).},
note = {68},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27; 39; 27 and 13 % of hydrocarbons added to culture medium were degraded; respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils. © 2014 The Author(s).
2012
Płaza, G. A.; Król, E.; Pacwa-Płociniczak, M.; Piotrowska-Seget, Z.; Brigmon, L. Robin
In: Acta Scientiarum Polonorum, Hortorum Cultus, vol. 11, no. 5, pp. 169-182, 2012, ISSN: 16440692, (5).
@article{2-s2.0-84879297069,
title = {Study of antifungal activity of Bacilli species cultured on agro-industrial wastes [Wstępne badania właściwości przeciwgrzybowych bakterii z rodzaju Bacillus rosnących na odpadach z przemysłu rolno-spożywczego]},
author = { G.A. Płaza and E. Król and M. Pacwa-Płociniczak and Z. Piotrowska-Seget and L. Robin Brigmon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84879297069&partnerID=40&md5=959fe23b8951ed25890f7221c8733828},
issn = {16440692},
year = {2012},
date = {2012-01-01},
journal = {Acta Scientiarum Polonorum, Hortorum Cultus},
volume = {11},
number = {5},
pages = {169-182},
publisher = {Wydawnictwo Akad Rolniczej W Lublinie},
abstract = {The three Bacillus species isolated from petroleum refinery waste were examined for antifungal activity on brewery effluents and molasses for biotechnological applications. Bacillus strains were identified by three different methods: 16S rRNA gene sequences, BIOLOG system and fatty acid analysis (FAME). The results demonstrated the ability of all three Bacillus strains cultured on brewery effluents and molasses to inhibit mycelial growth of the 10 tested fungi to varying degrees measured by agar plate inhibition assays. Fungi inhibited to the greatest degree as measured by the zones of inhibition were Botrytis cinerea A 258, Phomopsis viticola W 977, Septoria carvi K 2082, Colletotrichum gloeosporioides A 259, Phoma complanata A 233 and Phoma exigua var. exigua A 175. It was also observed that the fungal mycelial growth was inhibited by the cell-free supernatants, indicating lipoprotein-like activity of antifungal agents (mainly biosurfactants). Tested fungi were most sensitive to the Bacilli supernatants obtained from the molasses cultures including: B. cinerea A 258, R. solani W 70, S. sclerotiorum K 2291, Phomopsis diachenii K 657, C. dematium K 425, P. complanata A 233, P. exigua var. exigua A 175. In the previous study it was shown that Bacillus species produced biosurfactants. Application of natural products such as these Bacillus species or their byproducts may be a new approach to phytopathogen control therefore reducing the need for fungicides.},
note = {5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The three Bacillus species isolated from petroleum refinery waste were examined for antifungal activity on brewery effluents and molasses for biotechnological applications. Bacillus strains were identified by three different methods: 16S rRNA gene sequences, BIOLOG system and fatty acid analysis (FAME). The results demonstrated the ability of all three Bacillus strains cultured on brewery effluents and molasses to inhibit mycelial growth of the 10 tested fungi to varying degrees measured by agar plate inhibition assays. Fungi inhibited to the greatest degree as measured by the zones of inhibition were Botrytis cinerea A 258, Phomopsis viticola W 977, Septoria carvi K 2082, Colletotrichum gloeosporioides A 259, Phoma complanata A 233 and Phoma exigua var. exigua A 175. It was also observed that the fungal mycelial growth was inhibited by the cell-free supernatants, indicating lipoprotein-like activity of antifungal agents (mainly biosurfactants). Tested fungi were most sensitive to the Bacilli supernatants obtained from the molasses cultures including: B. cinerea A 258, R. solani W 70, S. sclerotiorum K 2291, Phomopsis diachenii K 657, C. dematium K 425, P. complanata A 233, P. exigua var. exigua A 175. In the previous study it was shown that Bacillus species produced biosurfactants. Application of natural products such as these Bacillus species or their byproducts may be a new approach to phytopathogen control therefore reducing the need for fungicides.
2011
Płaza, G. A.; Pacwa-Płociniczak, M.; Piotrowska-Seget, Z.; Jangid, K.; Wilk, K. A.
Agroindustrial wastes as unconventional substrates for growing of bacillus strains and production of biosurfactant Journal Article
In: Environment Protection Engineering, vol. 37, no. 3, pp. 63-71, 2011, ISSN: 03248828, (20).
@article{2-s2.0-80054732959,
title = {Agroindustrial wastes as unconventional substrates for growing of bacillus strains and production of biosurfactant},
author = { G.A. Płaza and M. Pacwa-Płociniczak and Z. Piotrowska-Seget and K. Jangid and K.A. Wilk},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80054732959&partnerID=40&md5=ad93e3a9b96ab17ae6df17304fa3f960},
issn = {03248828},
year = {2011},
date = {2011-01-01},
journal = {Environment Protection Engineering},
volume = {37},
number = {3},
pages = {63-71},
abstract = {The study was aimed at the development of economical methods for biosurfactant production by the use of unconventional substrates. The research investigated the potential of utilising agroindustrial wastes to replace synthetic media for cultivation of Bacillus strains and biosurfactant production. In total, 21 of the waste products from dairy, sugar, fatty, and fruit and vegetable processing industries, breweries, distillery were examined. Three bacterial strains were identified by 16S rRNA gene sequencing: Bacillus subtilis (I'-1a), Bacillus sp. (T-1), Bacillus sp. (T'-1). Biosurfactant production was examined in an indirect way by measuring of surface tension (ST), blood agar lysis, oil spreading and drop collapsing tests. The best unconventional substrates for bacteria growing and biosurfactant production at 30 °C under aerobic conditions were molasses, brewery effluents, and fruit and vegetable decoction from the processing factory.},
note = {20},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The study was aimed at the development of economical methods for biosurfactant production by the use of unconventional substrates. The research investigated the potential of utilising agroindustrial wastes to replace synthetic media for cultivation of Bacillus strains and biosurfactant production. In total, 21 of the waste products from dairy, sugar, fatty, and fruit and vegetable processing industries, breweries, distillery were examined. Three bacterial strains were identified by 16S rRNA gene sequencing: Bacillus subtilis (I'-1a), Bacillus sp. (T-1), Bacillus sp. (T'-1). Biosurfactant production was examined in an indirect way by measuring of surface tension (ST), blood agar lysis, oil spreading and drop collapsing tests. The best unconventional substrates for bacteria growing and biosurfactant production at 30 °C under aerobic conditions were molasses, brewery effluents, and fruit and vegetable decoction from the processing factory.
Pacwa-Płociniczak, M.; Płaza, G. A.; Piotrowska-Seget, Z.; Cameotra, S. S.
Environmental applications of biosurfactants: Recent advances Journal Article
In: International Journal of Molecular Sciences, vol. 12, no. 1, pp. 633-654, 2011, ISSN: 14220067, (588).
@article{2-s2.0-79251615424,
title = {Environmental applications of biosurfactants: Recent advances},
author = { M. Pacwa-Płociniczak and G.A. Płaza and Z. Piotrowska-Seget and S.S. Cameotra},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79251615424&doi=10.3390%2fijms12010633&partnerID=40&md5=64c23c77b76235144c093a9cd650bd11},
doi = {10.3390/ijms12010633},
issn = {14220067},
year = {2011},
date = {2011-01-01},
journal = {International Journal of Molecular Sciences},
volume = {12},
number = {1},
pages = {633-654},
abstract = {Increasing public awareness of environmental pollution influences the search and development of technologies that help in clean up of organic and inorganic contaminants such as hydrocarbons and metals. An alternative and eco-friendly method of remediation technology of environments contaminated with these pollutants is the use of biosurfactants and biosurfactant-producing microorganisms. The diversity of biosurfactants makes them an attractive group of compounds for potential use in a wide variety of industrial and biotechnological applications. The purpose of this review is to provide a comprehensive overview of advances in the applications of biosurfactants and biosurfactant-producing microorganisms in hydrocarbon and metal remediation technologies. © 2010 by the authors; licensee MDPI, Basel, Switzerland.},
note = {588},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Increasing public awareness of environmental pollution influences the search and development of technologies that help in clean up of organic and inorganic contaminants such as hydrocarbons and metals. An alternative and eco-friendly method of remediation technology of environments contaminated with these pollutants is the use of biosurfactants and biosurfactant-producing microorganisms. The diversity of biosurfactants makes them an attractive group of compounds for potential use in a wide variety of industrial and biotechnological applications. The purpose of this review is to provide a comprehensive overview of advances in the applications of biosurfactants and biosurfactant-producing microorganisms in hydrocarbon and metal remediation technologies. © 2010 by the authors; licensee MDPI, Basel, Switzerland.