@article{2-s2.0-85183529285,

title = {Discovery and characterization of the α-amylases cDNAs from Enchytraeus albidus shed light on the evolution of “Enchytraeus-Eisenia type” Amy homologs in Annelida},

author = { Ł. Gajda and A. Daszkowska-Golec and P. Świątek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85183529285&doi=10.1016%2fj.biochi.2024.01.008&partnerID=40&md5=f3e1ede4555d73b59ba6142e83818df9},

doi = {10.1016/j.biochi.2024.01.008},

issn = {03009084},

year  = {2024},

date = {2024-01-01},

journal = {Biochimie},

volume = {221},

pages = {38-59},

publisher = {Elsevier B.V.},

abstract = {Although enchytraeids have gained popularity in scientific research, fundamental questions regarding their feeding ecology and biology remain largely unexplored. This study investigates α-amylases, major digestive enzymes responsible for hydrolyzing starch and similar polysaccharides into sugars, in Enchytraeus albidus. Genetic data related to α-amylases is currently lacking for the family Enchytraeidae but also for the entire Annelida. To detect and identify coding sequences of the expressed α-amylase genes in COI-monohaplotype culture (PL-A strain) of E. albidus, we used classical “gene fishing” and transcriptomic approaches. We also compared coding sequence variants of α-amylase retrieved from transcriptomic data related to freeze-tolerant strains. Our results reveal that E. albidus possesses two distinct α-amylase genes (Amy I and Amy II) that are homologs to earthworm Eisenia fetida Ef-Amy genes. Different strains of E. albidus possess distinctive alleles of α-amylases with unique SNP patterns specific to a particular strain. Unlike Amy II, Amy I seems to be a highly polymorphic and multicopy gene. The domain architecture of the putative Amy proteins was found the same as for classical animal α-amylases with ABC-domains. A characteristic feature of Amy II is the lack of GHGA motif in the flexible loop region, similarly to many insect amylases. We identified “Enchytraeus-Eisenia type” α-amylase homologs in other clitellates and polychaetes, indicating the ancestral origin of Amy I/II proteins in Annelida. This study provides the first insight into the endogenous non-proteolytic digestive enzyme genes in potworms, discusses the evolution of Amy α-amylases in Annelida, and explores phylogenetic implications. © 2024 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM)},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85178353935,

title = {Enhancing climate change resilience in agricultural crops},

author = { Y. Benitez-Alfonso and B.K. Soanes and S. Zimba and B. Sinanaj and L. German and V.K. Sharma and A.K. Bohra and A. Kolesnikova and J.A. Dunn and A.C. Martin and M.U. Khashi U Rahman and Z. Saati-Santamaría and P. García-Fraile and E.A. Ferreira and T. Ben Hadda and W.A. Cowling and K.H. Siddique and M.K. Pandey and M. Farooq and R.K. Varshney and M.A. Chapman and C. Boesch and A. Daszkowska-Golec and C.H. FOYER},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85178353935&doi=10.1016%2fj.cub.2023.10.028&partnerID=40&md5=c2523d494e3a070affab4b2a81f7400d},

doi = {10.1016/j.cub.2023.10.028},

issn = {09609822},

year  = {2023},

date = {2023-01-01},

journal = {Current Biology},

volume = {33},

number = {23},

pages = {R1246-R1261},

publisher = {Cell Press},

abstract = {Climate change threatens global food and nutritional security through negative effects on crop growth and agricultural productivity. Many countries have adopted ambitious climate change mitigation and adaptation targets that will exacerbate the problem, as they require significant changes in current agri-food systems. In this review, we provide a roadmap for improved crop production that encompasses the effective transfer of current knowledge into plant breeding and crop management strategies that will underpin sustainable agriculture intensification and climate resilience. We identify the main problem areas and highlight outstanding questions and potential solutions that can be applied to mitigate the impacts of climate change on crop growth and productivity. Although translation of scientific advances into crop production lags far behind current scientific knowledge and technology, we consider that a holistic approach, combining disciplines in collaborative efforts, can drive better connections between research, policy, and the needs of society. © 2023 The Authors},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85173652699,

title = {Molecular mechanisms of SNAC1 (Stress-responsive NAC1) in conferring the abiotic stress tolerance},

author = { M.M. Kurowska and A. Daszkowska-Golec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85173652699&doi=10.1016%2fj.plantsci.2023.111894&partnerID=40&md5=d43a797e2ba83efab5adf3b1cf3685e5},

doi = {10.1016/j.plantsci.2023.111894},

issn = {01689452},

year  = {2023},

date = {2023-01-01},

journal = {Plant Science},

volume = {337},

publisher = {Elsevier Ireland Ltd},

abstract = {NAC family gene - SNAC1 (Stress-responsive NAC1) is responsive to drought, salt, cold stress, and ABA. It acts as a regulator in mediating tolerance to abiotic stress through different pathways. Abiotic stress, among them drought and salinity, are adverse factors for plant growth and crop productivity. SNAC1 was an object of high interest according to the effect of improved drought and salt tolerance when overexpressed in different plant species such as rice, wheat, barley, cotton, maize, banana, or oat. SNAC1 functions by regulating the expression of genes that contain the NAC Recognized Sequence (NACRS) within their promoter region. This gene is induced by drought, specifically in guard cells. Its downstream targets have been identified. The role of SNAC1 in molecular and physiological responses during abiotic stress has been proposed, but this knowledge still needs to be expanded. Here, we describe recent advances in understanding the action of SNAC1 in adapting plants to abiotic stress. © 2023},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85171857887,

title = {Multi-omics insights into the positive role of strigolactone perception in barley drought response},

author = { A. Daszkowska-Golec and D. Mehta and R.G. Uhrig and A.J. Brąszewska-Zalewska and O. Novák and I.M. Fontana and M. Melzer and T. Płociniczak and M. Marzec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85171857887&doi=10.1186%2fs12870-023-04450-1&partnerID=40&md5=b4cdbd82982d8b64f033dd023c188683},

doi = {10.1186/s12870-023-04450-1},

issn = {14712229},

year  = {2023},

date = {2023-01-01},

journal = {BMC Plant Biology},

volume = {23},

number = {1},

publisher = {BioMed Central Ltd},

abstract = {Background: Drought is a major environmental stress that affects crop productivity worldwide. Although previous research demonstrated links between strigolactones (SLs) and drought, here we used barley (Hordeum vulgare) SL-insensitive mutant hvd14 (dwarf14) to scrutinize the SL-dependent mechanisms associated with water deficit response. Results: We have employed a combination of transcriptomics, proteomics, phytohormonomics analyses, and physiological data to unravel differences between wild-type and hvd14 plants under drought. Our research revealed that drought sensitivity of hvd14 is related to weaker induction of abscisic acid-responsive genes/proteins, lower jasmonic acid content, higher reactive oxygen species content, and lower wax biosynthetic and deposition mechanisms than wild-type plants. In addition, we identified a set of transcription factors (TFs) that are exclusively drought-induced in the wild-type barley. Conclusions: Critically, we resolved a comprehensive series of interactions between the drought-induced barley transcriptome and proteome responses, allowing us to understand the profound effects of SLs in alleviating water-limiting conditions. Several new avenues have opened for developing barley more resilient to drought through the information provided. Moreover, our study contributes to a better understanding of the complex interplay between genes, proteins, and hormones in response to drought, and underscores the importance of a multidisciplinary approach to studying plant stress response mechanisms. © 2023, BioMed Central Ltd., part of Springer Nature.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85159675297,

title = {A complex signaling trio in seed germination: Auxin-JA-ABA},

author = { E. Sybilska and A. Daszkowska-Golec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85159675297&doi=10.1016%2fj.tplants.2023.05.003&partnerID=40&md5=f414487d157463562f983b990aaa08dc},

doi = {10.1016/j.tplants.2023.05.003},

issn = {13601385},

year  = {2023},

date = {2023-01-01},

journal = {Trends in Plant Science},

volume = {28},

number = {8},

pages = {873-875},

publisher = {Elsevier Ltd},

abstract = {Recently. Mei et al. discovered the molecular mechanism behind the synergistic action of auxins and jasmonates in enhancing the role of abscisic acid (ABA) in seed germination. They found that JASMONATE-ZIM DOMAIN (JAZ) proteins interact with AUXIN RESPONSE FACTOR (ARF)-16 to mediate auxin–jasmonic acid (JA) crosstalk. Furthermore, they revealed that ARF16 interacts with ABSCISIC ACID INSENSITIVE (ABI)-5 and positively modulates ABA responses at seed germination. © 2023 Elsevier Ltd},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85148702118,

title = {PlantACT! – how to tackle the climate crisis},

author = { A. Martin and A. Daszkowska-Golec and E.M. Gómez-Álvarez and C.A. Walsh and V.S. Kamenetsky and S. Al-Babili and M. Almeida-Trapp and M. Aranda and D. Bartels and M.J. Bennett and I. Blilou and D. Boer and A. Boulouis and C.P. Bowler and S. Brunel-Muguet and F. Chardon and J. Colcombet and V. Colot and J.R. DInneny and B. Field and K. Froehlich and C.H. Gardener and A. Gojon and E. Gomès and C. Gutierrez and M. Havaux and S. Hayes and É. Heard and M. Hodges and A. Stra and L. Laplaze and K.J. Lauersen and N. Leonhardt and X. Johnson and J.D. Jones and H. Kollist and S. Kopriva and V.S. Kamenetsky and M.L.P. Masson and M.F. McCabe and L. Merendino and A. Molina and J.L. Moreno Ramirez and B. Mueller-Roeber and M. Nicolas and I. Nir and I.O. Orduna and J.M. Pardo and J.P. Reichheld and P.L. Rodríguez and H. Rouached and M.M. Saad and P. Schlögelhofer and K.A. Singh and Authors. Other},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85148702118&doi=10.1016%2fj.tplants.2023.01.005&partnerID=40&md5=7ad226e4bbc1b9dce469cb5e618ecf4c},

doi = {10.1016/j.tplants.2023.01.005},

issn = {13601385},

year  = {2023},

date = {2023-01-01},

journal = {Trends in Plant Science},

volume = {28},

number = {5},

pages = {537-543},

publisher = {Elsevier Ltd},

abstract = {Greenhouse gas (GHG) emissions have created a global climate crisis which requires immediate interventions to mitigate the negative effects on all aspects of life on this planet. As current agriculture and land use contributes up to 25% of total GHG emissions, plant scientists take center stage in finding possible solutions for a transition to sustainable agriculture and land use. In this article, the PlantACT! (Plants for climate ACTion!) initiative of plant scientists lays out a road map of how and in which areas plant scientists can contribute to finding immediate, mid-term, and long-term solutions, and what changes are necessary to implement these solutions at the personal, institutional, and funding levels. © 2023 Elsevier Ltd 

Authors: Kamenetsky, V.S.; Al-Babili, S.; Almeida-Trapp, M.; Martin, A.; Aranda, M.; Bartels, D.; Bennett, M.J.; Blilou, I.; Boer, D.; Boulouis, A.; Bowler, C.P.; Brunel-Muguet, S.; Chardon, F.; Colcombet, J.; Colot, V.; Daszkowska-Golec, A.; DInneny, J.R.; Field, B.; Froehlich, K.; Gardener, C.H.; Gojon, A.; Gomès, E.; Gómez-Álvarez, E.M.; Gutierrez, C.; Havaux, M.; Hayes, S.; Heard, É.; Hodges, M.; Stra, A.; Laplaze, L.; Lauersen, K.J.; Leonhardt, N.; Johnson, X.; Jones, J.D.; Kollist, H.; Kopriva, S.; Kamenetsky, V.S.; Masson, M.L.P.; McCabe, M.F.; Merendino, L.; Molina, A.; Moreno Ramirez, J.L.; Mueller-Roeber, B.; Nicolas, M.; Nir, I.; Orduna, I.O.; Pardo, J.M.; Reichheld, J.P.; Rodríguez, P.L.; Rouached, H.; Saad, M.M.; Schlögelhofer, P.; Singh, K.A.; de Smet, I.; Stanschewski, C.S.; Stra, A.; Tester, M.; Walsh, C.A.; Weber, A.P.M.; Weigel, D.; Wigge, P.A.; Wrzaczek, M.; Wulff, B.B.H.; Young, I.M.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85175865808,

title = {Drought response of water-conserving and non-conserving spring barley cultivars},

author = { M. Appiah and I. Abdulai and A.H. Schulman and M. Moshelion and E.S. Dewi and A. Daszkowska-Golec and G. Bracho-Mujica and R.P. Rötter},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85175865808&doi=10.3389%2ffpls.2023.1247853&partnerID=40&md5=096d18d4303d373a0e3e35fd6099591f},

doi = {10.3389/fpls.2023.1247853},

issn = {1664462X},

year  = {2023},

date = {2023-01-01},

journal = {Frontiers in Plant Science},

volume = {14},

publisher = {Frontiers Media SA},

abstract = {Introduction: Breeding barley cultivars adapted to drought requires in-depth knowledge on physiological drought responses. Methods: We used a high-throughput functional phenotyping platform to examine the response of four high-yielding European spring barley cultivars to a standardized drought treatment imposed around flowering. Results: Cv. Chanell showed a non-conserving water-use behavior with high transpiration and maximum productivity under well-watered conditions but rapid transpiration decrease under drought. The poor recovery upon re-irrigation translated to large yield losses. Cv. Baronesse showed the most water-conserving behavior, with the lowest pre-drought transpiration and the most gradual transpiration reduction under drought. Its good recovery (resilience) prevented large yield losses. Cv. Formula was less conserving than cv. Baronesse and produced low yet stable yields. Cv. RGT’s dynamic water use with high transpiration under ample water supply and moderate transpiration decrease under drought combined with high resilience secured the highest and most stable yields. Discussion: Such a dynamic water-use behavior combined with higher drought resilience and favorable root traits could potentially create an ideotype for intermediate drought. Prospective studies will examine these results in field experiments and will use the newly gained understanding on water use in barley to improve process descriptions in crop simulation models to support crop model–aided ideotype design. Copyright © 2023 Appiah, Abdulai, Schulman, Moshelion, Dewi, Daszkowska-Golec, Bracho-Mujica and Rötter.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85174266396,

title = {Update on stomata development and action under abiotic stress},

author = { H. Matkowski and A. Daszkowska-Golec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85174266396&doi=10.3389%2ffpls.2023.1270180&partnerID=40&md5=b3d7879fdd7319da65a2a3fe5c3b00d5},

doi = {10.3389/fpls.2023.1270180},

issn = {1664462X},

year  = {2023},

date = {2023-01-01},

journal = {Frontiers in Plant Science},

volume = {14},

publisher = {Frontiers Media SA},

abstract = {Stomata, key gatekeepers of plant hydration, have long been known to play a pivotal role in mitigating the impacts of abiotic stressors. However, the complex molecular mechanisms underscoring this role remain unresolved fully and continue to be the subject of research. In the context of water-use efficiency (WUE), a key indicator of a plant’s ability to conserve water, this aspect links intrinsically with stomatal behavior. Given the pivotal role of stomata in modulating water loss, it can be argued that the complex mechanisms governing stomatal development and function will significantly influence a plant’s WUE under different abiotic stress conditions. Addressing these calls for a concerted effort to strengthen plant adaptability through advanced, targeted research. In this vein, recent studies have illuminated how specific stressors trigger alterations in gene expression, orchestrating changes in stomatal pattern, structure, and opening. This reveals a complex interplay between stress stimuli and regulatory sequences of essential genes implicated in stomatal development, such as MUTE, SPCH, and FAMA. This review synthesizes current discoveries on the molecular foundations of stomatal development and behavior in various stress conditions and their implications for WUE. It highlights the imperative for continued exploration, as understanding and leveraging these mechanisms guarantee enhanced plant resilience amid an ever-changing climatic landscape. Copyright © 2023 Matkowski and Daszkowska-Golec.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85151957441,

title = {Alternative splicing in ABA signaling during seed germination},

author = { E. Sybilska and A. Daszkowska-Golec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151957441&doi=10.3389%2ffpls.2023.1144990&partnerID=40&md5=f92e1abe3eeaba0a984d519942c54672},

doi = {10.3389/fpls.2023.1144990},

issn = {1664462X},

year  = {2023},

date = {2023-01-01},

journal = {Frontiers in Plant Science},

volume = {14},

publisher = {Frontiers Media S.A.},

abstract = {Seed germination is an essential step in a plant’s life cycle. It is controlled by complex physiological, biochemical, and molecular mechanisms and external factors. Alternative splicing (AS) is a co-transcriptional mechanism that regulates gene expression and produces multiple mRNA variants from a single gene to modulate transcriptome diversity. However, little is known about the effect of AS on the function of generated protein isoforms. The latest reports indicate that alternative splicing (AS), the relevant mechanism controlling gene expression, plays a significant role in abscisic acid (ABA) signaling. In this review, we present the current state of the art about the identified AS regulators and the ABA-related changes in AS during seed germination. We show how they are connected with the ABA signaling and the seed germination process. We also discuss changes in the structure of the generated AS isoforms and their impact on the functionality of the generated proteins. Also, we point out that the advances in sequencing technology allow for a better explanation of the role of AS in gene regulation by more accurate detection of AS events and identification of full-length splicing isoforms. Copyright © 2023 Sybilska and Daszkowska-Golec.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85125864352,

title = {High-throughput sequencing data revealed genotype-specific changes evoked by heat stress in crown tissue of barley sdw1 near-isogenic lines},

author = { K. Mikołajczak and A. Kuczyńska and P. Ogrodowicz and A. Kiełbowicz-Matuk and H. Ćwiek-Kupczyńska and A. Daszkowska-Golec and I. Szarejko and M. Surma and P. Krajewski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125864352&doi=10.1186%2fs12864-022-08410-1&partnerID=40&md5=d15aa50c188831a722d48e9e128f36d4},

doi = {10.1186/s12864-022-08410-1},

issn = {14712164},

year  = {2022},

date = {2022-01-01},

journal = {BMC Genomics},

volume = {23},

number = {1},

publisher = {BioMed Central Ltd},

abstract = {Background: High temperature shock is becoming increasingly common in our climate, affecting plant growth and productivity. The ability of a plant to survive stress is a complex phenomenon. One of the essential tissues for plant performance under various environmental stimuli is the crown. However, the molecular characterization of this region remains poorly investigated. Gibberellins play a fundamental role in whole-plant stature formation. This study identified plant stature modifications and crown-specific transcriptome re-modeling in gibberellin-deficient barley sdw1.a (BW827) and sdw1.d (BW828) mutants exposed to increased temperature. Results: The deletion around the sdw1 gene in BW827 was found to encompass at least 13 genes with primarily regulatory functions. A bigger genetic polymorphism of BW828 than of BW827 in relation to wild type was revealed. Transcriptome-wide sequencing (RNA-seq) revealed several differentially expressed genes involved in gibberellin metabolism and heat response located outside of introgression regions. It was found that HvGA20ox4, a paralogue of the HvGA20ox2 gene, was upregulated in BW828 relative to other genotypes, which manifested as basal internode elongation. The transcriptome response to elevated temperature differed in the crown of sdw1.a and sdw1.d mutants; it was most contrasting for HvHsf genes upregulated under elevated temperature in BW828, whereas those specific to BW827 were downregulated. In-depth examination of sdw1 mutants revealed also some differences in their phenotypes and physiology. Conclusions: We concluded that despite the studied sdw1 mutants being genetically related, their heat response seemed to be genotype-specific and observed differences resulted from genetic background diversity rather than single gene mutation, multiple gene deletion, or allele-specific expression of the HvGA20ox2 gene. Differences in the expressional reaction of genes to heat in different sdw1 mutants, found to be independent of the polymorphism, could be further explained by in-depth studies of the regulatory factors acting in the studied system. Our findings are particularly important in genetic research area since molecular response of crown tissue has been marginally investigated, and can be useful for wide genetic research of crops since barley has become a model plant for them. © 2022, The Author(s).},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85125669610,

title = {ABA is important not only under stress – revealed by the discovery of new ABA transporters},

author = { A. Daszkowska-Golec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125669610&doi=10.1016%2fj.tplants.2022.02.006&partnerID=40&md5=26d8c971c8ec78871a7872dd5c8ad4ab},

doi = {10.1016/j.tplants.2022.02.006},

issn = {13601385},

year  = {2022},

date = {2022-01-01},

journal = {Trends in Plant Science},

volume = {27},

number = {5},

pages = {423-425},

publisher = {Elsevier Ltd},

abstract = {Recent reports by Qin et al. and Zhang et al. cast light on long-distance abscisic acid (ABA) transport and demonstrate that ABA has a major impact on plant growth and physiology under both non-stress and stress conditions. Insights into ABA transport may provide a means to modulate the response of plants to climate change. © 2022 Elsevier Ltd},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85121685217,

title = {The landscape of plant genomics after 20 years},

author = { A. Daszkowska-Golec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121685217&doi=10.1016%2fj.tig.2021.12.004&partnerID=40&md5=35a83985be84bc9538a0e90117c5e131},

doi = {10.1016/j.tig.2021.12.004},

issn = {01689525},

year  = {2022},

date = {2022-01-01},

journal = {Trends in Genetics},

volume = {38},

number = {4},

pages = {310-311},

publisher = {Elsevier Ltd},

abstract = {Many letters of the plant genetic code have been read since the first plant genome was published in 2000 for Arabidopsis. In a recent paper, Marks et al. examined 798 plant genomes and provide a current view of plant genomics in terms of the quality of the assemblies, coupled with their taxonomical and geographical distribution. © 2021 Elsevier Ltd},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85115044224,

title = {Updates on the role of ABSCISIC ACID INSENSITIVE 5 (ABI5) and ABSCISIC ACID-RESPONSIVE ELEMENT BINDING FACTORS (ABFs) in ABA signaling in different developmental stages in plants},

author = { A. Collin and A. Daszkowska-Golec and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115044224&doi=10.3390%2fcells10081996&partnerID=40&md5=288aae4e7889cefdab714eb59b407ca5},

doi = {10.3390/cells10081996},

issn = {20734409},

year  = {2021},

date = {2021-01-01},

journal = {Cells},

volume = {10},

number = {8},

publisher = {MDPI},

abstract = {The core abscisic acid (ABA) signaling pathway consists of receptors, phosphatases, ki-nases and transcription factors, among them ABA INSENSITIVE 5 (ABI5) and ABRE BINDING FACTORs/ABRE-BINDING PROTEINs (ABFs/AREBs), which belong to the BASIC LEUCINE ZIPPER (bZIP) family and control expression of stress-responsive genes. ABI5 is mostly active in seeds and prevents germination and post-germinative growth under unfavorable conditions. The activity of ABI5 is controlled at transcriptional and protein levels, depending on numerous regulators, including components of other phytohormonal pathways. ABFs/AREBs act redundantly in regulating genes that control physiological processes in response to stress during vegetative growth. In this review, we focus on recent reports regarding ABI5 and ABFs/AREBs functions during abiotic stress responses, which seem to be partially overlapping and not restricted to one developmental stage in Arabidopsis and other species. Moreover, we point out that ABI5 and ABFs/AREBs play a crucial role in the core ABA pathway’s feedback regulation. In this review, we also discuss increased stress tolerance of transgenic plants overexpressing genes encoding ABA-dependent bZIPs. Taken to-gether, we show that ABI5 and ABFs/AREBs are crucial ABA-dependent transcription factors regulating processes essential for plant adaptation to stress at different developmental stages. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85099656002,

title = {Identification of the genetic basis of response to de-acclimation in winter barley},

author = { M. Wójcik-Jagła and A. Daszkowska-Golec and A. Fiust and P. Kopeć and M. Rapacz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099656002&doi=10.3390%2fijms22031057&partnerID=40&md5=72c633755f236c952310992fe3002749},

doi = {10.3390/ijms22031057},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {3},

pages = {1-33},

publisher = {MDPI AG},

abstract = {Mechanisms involved in the de-acclimation of herbaceous plants caused by warm periods during winter are poorly understood. This study identifies the genes associated with this mechanism in winter barley. Seedlings of eight accessions (four tolerant and four susceptible to de-acclimation cultivars and advanced breeding lines) were cold acclimated for three weeks and de-acclimated at 12 °C/5 °C (day/night) for one week. We performed differential expression analysis using RNA sequencing. In addition, reverse-transcription quantitative real-time PCR and enzyme activity analyses were used to investigate changes in the expression of selected genes. The number of transcripts with accumulation level changed in opposite directions during acclimation and de-acclimation was much lower than the number of transcripts with level changed exclusively during one of these processes. The de-acclimation-susceptible accessions showed changes in the expression of a higher number of functionally diverse genes during de-acclimation. Transcripts associated with stress response, especially oxidoreductases, were the most abundant in this group. The results provide novel evidence for the distinct molecular regulation of cold acclimation and de-acclimation. Upregulation of genes controlling developmental changes, typical for spring de-acclimation, was not observed during mid-winter de-acclimation. Mid-winter de-acclimation seems to be perceived as an opportunity to regenerate after stress. Unfortunately, it is competitive to remain in the cold-acclimated state. This study shows that the response to mid-winter de-acclimation is far more expansive in de-acclimation-susceptible cultivars, suggesting that a reduced response to the rising temperature is crucial for de-acclimation tolerance. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85089696682,

title = {Cuticular waxes—A shield of barley mutant in CBP20 (Cap-Binding Protein 20) gene when struggling with drought stress},

author = { A. Daszkowska-Golec and J. Karcz and T. Płociniczak and K. Sitko and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089696682&doi=10.1016%2fj.plantsci.2020.110593&partnerID=40&md5=ad2913c77b8570c0d656e8236defcf56},

doi = {10.1016/j.plantsci.2020.110593},

issn = {01689452},

year  = {2020},

date = {2020-01-01},

journal = {Plant Science},

volume = {300},

publisher = {Elsevier Ireland Ltd},

abstract = {CBP20 (Cap-Binding Protein 20) encodes a small subunit of nuclear Cap-Binding Complex (nCBC) that together with CBP80 binds mRNA cap. We previously described barley hvcbp20.ab mutant that demonstrated higher leaf water content and faster stomatal closure than the WT after drought stress. Hence, we presumed that the better water-saving mechanism in hvcbp20.ab may result from the lower permeability of epidermis that together with stomata action limit the water evaporation under drought stress. We asked whether hvcbp20.ab exhibited any differences in wax load on the leaf surface when subjected to drought in comparison to WT cv. ‘Sebastian’. To address this question, we investigated epicuticular wax structure and chemical composition under drought stress in hvcbp20.ab mutant and its WT. We showed that hvcbp20.ab mutant exhibited the increased deposition of cuticular wax. Moreover, our gene expression results suggested a role of HvCBP20 as a negative regulator of both, the biosynthesis of waxes at the level of alkane-forming, and waxes transportation. Interestingly, we also observed increased wax deposition in Arabidopsis cbp20 mutant exposed to drought, which allowed us to describe the CBP20-regulated epicuticular wax accumulation under drought stress in a wider evolutionarily context. © 2020 Elsevier B.V.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85087406726,

title = {Degrade or Silence? – RNA Turnover Takes Control of Epicuticular Wax Synthesis},

author = { A. Daszkowska-Golec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087406726&doi=10.1016%2fj.tplants.2020.06.009&partnerID=40&md5=90fdc13a1f288fe97a1abc95d1229ab1},

doi = {10.1016/j.tplants.2020.06.009},

issn = {13601385},

year  = {2020},

date = {2020-01-01},

journal = {Trends in Plant Science},

volume = {25},

number = {10},

pages = {950-952},

publisher = {Elsevier Ltd},

abstract = {Epicuticular waxes serve as a protective layer on plant aerial surfaces. The chemical reactions of wax biosynthesis are well understood, but little is known about the underlying regulatory mechanisms. New data from Yang et al. and Lange et al. argue that RNA degradation and silencing play a key role in regulating the content of alkanes, the most abundant components of wax. © 2020 Elsevier Ltd},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85088304268,

title = {Barley strigolactone signalling mutant hvd14.d reveals the role of strigolactones in abscisic acid-dependent response to drought},

author = { M. Marzec and A. Daszkowska-Golec and A. Collin and M. Melzer and K. Eggert and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088304268&doi=10.1111%2fpce.13815&partnerID=40&md5=a17d98cfe41937b5140917b7f9836499},

doi = {10.1111/pce.13815},

issn = {01407791},

year  = {2020},

date = {2020-01-01},

journal = {Plant Cell and Environment},

volume = {43},

number = {9},

pages = {2239-2253},

publisher = {Blackwell Publishing Ltd},

abstract = {Strigolactones (SLs) are a group of plant hormones involved in many aspects of plant development and stress adaptation. Here, we investigated the drought response of a barley (Hordeum vulgare L.) mutant carrying a missense mutation in the gene encoding the SL-specific receptor HvD14. Our results clearly showed that hvd14.d mutant is hyper-sensitive to drought stress. This was illustrated by a lower leaf relative water content (RWC), impaired photosynthesis, disorganization of chloroplast structure, altered stomatal density and slower closure of stomata in response to drought in the mutant compared to the wild type parent cultivar Sebastian. Although the content of abscisic acid (ABA) and its derivatives remained unchanged in the mutant, significant differences in expression of genes related to ABA biosynthesis were observed. Moreover, hvd14.d was insensitive to ABA during seed germination. Analysis of Arabidopsis thaliana mutant atd14-1 also demonstrated that mutation in the SL receptor resulted in increased sensitivity to drought. Our results indicate that the drought-sensitive phenotype of barley SL mutant might be caused by a disturbed ABA metabolism and/or signalling pathways. These results together uncovered a link between SL signalling and ABA-dependent drought stress response in barley. © 2020 John Wiley & Sons Ltd.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85089435133,

title = {Barley ABI5 (Abscisic Acid INSENSITIVE 5) Is Involved in Abscisic Acid-Dependent Drought Response},

author = { A. Collin and A. Daszkowska-Golec and M.M. Kurowska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089435133&doi=10.3389%2ffpls.2020.01138&partnerID=40&md5=ab2494c5d3f9cd41dd76be0da41911a1},

doi = {10.3389/fpls.2020.01138},

issn = {1664462X},

year  = {2020},

date = {2020-01-01},

journal = {Frontiers in Plant Science},

volume = {11},

publisher = {Frontiers Media S.A.},

abstract = {ABA INSENSITIVE 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor which acts in the abscisic acid (ABA) network and is activated in response to abiotic stresses. However, the precise role of barley (Hordeum vulgare) ABI5 in ABA signaling and its function under stress remains elusive. Here, we show that HvABI5 is involved in ABA-dependent regulation of barley response to drought stress. We identified barley TILLING mutants carrying different alleles in the HvABI5 gene and we studied in detail the physiological and molecular response to drought and ABA for one of them. The hvabi5.d mutant, carrying G1751A transition, was insensitive to ABA during seed germination, yet it showed the ability to store more water than its parent cv. “Sebastian” (WT) in response to drought stress. The drought-tolerant phenotype of hvabi5.d was associated with better membrane protection, higher flavonoid content, and faster stomatal closure in the mutant under stress compared to the WT. The microarray transcriptome analysis revealed up-regulation of genes associated with cell protection mechanisms in the mutant. Furthermore, HvABI5 target genes: HVA1 and HVA22 showed higher activity after drought, which may imply better adaptation of hvabi5.d to stress. On the other hand, chlorophyll content in hvabi5.d was lower than in WT, which was associated with decreased photosynthesis efficiency observed in the mutant after drought treatment. To verify that HvABI5 acts in the ABA-dependent manner we analyzed expression of selected genes related to ABA pathway in hvabi5.d and its WT parent after drought and ABA treatments. The expression of key genes involved in ABA metabolism and signaling differed in the mutant and the WT under stress. Drought-induced increase of expression of HvNCED1, HvBG8, HvSnRK2.1, and HvPP2C4 genes was 2–20 times higher in hvabi5.d compared to “Sebastian”. We also observed a faster stomatal closure in hvabi5.d and much higher induction of HvNCED1 and HvSnRK2.1 genes after ABA treatment. Together, these findings demonstrate that HvABI5 plays a role in regulation of drought response in barley and suggest that HvABI5 might be engaged in the fine tuning of ABA signaling by a feedback regulation between biosynthetic and signaling events. In addition, they point to different mechanisms of HvABI5 action in regulating drought response and seed germination in barley. © Copyright © 2020 Collin, Daszkowska-Golec, Kurowska and Szarejko.},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85086678590,

title = {Methyl jasmonate affects photosynthesis efficiency, expression of HvTIP genes and nitrogen homeostasis in Barley},

author = { M.M. Kurowska and A. Daszkowska-Golec and M. Gajecka and P. Kościelniak and W.M. Bierza and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086678590&doi=10.3390%2fijms21124335&partnerID=40&md5=388ee2567db937bac0ac87ab71487383},

doi = {10.3390/ijms21124335},

issn = {16616596},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Molecular Sciences},

volume = {21},

number = {12},

pages = {1-23},

publisher = {MDPI AG},

abstract = {Jasmonates modulate many growth and developmental processes and act as stress hormones that play an important role in plant tolerance to biotic and abiotic stresses. Therefore, there is a need to identify the genes that are regulated through the jasmonate signalling pathway. Aquaporins, and among them the Tonoplast Intrinsic Proteins (TIPs), form the channels in cell membranes that are responsible for the precise regulation of the movement of water and other substrates between cell compartments. We identified the cis-regulatory motifs for the methyl jasmonate (MeJA)-induced genes in the promoter regions of all the HvTIP genes, which are active in barley seedlings, and thus we hypothesised that the HvTIP expression could be a response to jasmonate signalling. In the presented study, we determined the effect of methyl jasmonate on the growth parameters and photosynthesis efficiency of barley seedlings that had been exposed to different doses of MeJA (15–1000 µM × 120 h) in a hydroponic solution. All of the applied MeJA concentrations caused a significant reduction of barley seedling growth, which was most evident in the length of the first leaf sheath and dry leaf weight. The observed decrease of the PSII parameters after the exposure to high doses of MeJA (500 µM or higher) was associated with the downregulation of HvPsbR gene encoding one of the extrinsic proteins of the Oxygen Evolving Complex. The reduced expression of HvPsbR might lead to the impairment of the OEC action, manifested by the occurrence of the K-band in an analysis of fluorescence kinetics after MeJA treatment as well as reduced photosynthesis efficiency. Furthermore, methyl jasmonate treatment caused a decrease in the nitrogen content in barley leaves, which was associated with an increased expression the four tonoplast aquaporin genes (HvTIP1;2; HvTIP2;2; HvTIP4;1 and HvTIP4;2) predicted to transport the nitrogen compounds from the vacuole to the cytosol. The upregulation of the nitrogen-transporting HvTIPs might suggest their involvement in the vacuolar unloading of ammonia and urea, which both could be remobilised when the nitrogen content in the leaves decreases. Our research provides tips on physiological role of the individual TIP subfamily members of aquaporins under methyl jasmonate action. © 2020 by the authors.Licensee MDPI, Basel, Switzerland.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85085360825,

title = {Development and aging of photosynthetic apparatus of vitis vinifera L. During growing season},

author = { K. Sitko and S. Rusinowski and M. Pogrzeba and A. Daszkowska-Golec and Ż. Gieroń and H.M. Kalaji and E. Małkowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085360825&doi=10.32615%2fps.2019.107&partnerID=40&md5=915a57b5dae35616a9b4abf2b8edbb85},

doi = {10.32615/ps.2019.107},

issn = {03003604},

year  = {2020},

date = {2020-01-01},

journal = {Photosynthetica},

volume = {58},

number = {Special Issue},

pages = {186-193},

publisher = {Institute of Experimental Botany, ASCR},

abstract = {The aim of this study was to examine the development and aging of chosen grapevine leaves in situ during the growing season (130 d) using chlorophyll (Chl) a fluorescence measurements and determining the changes in pigment contents. During the course of photosystems development, the increase of Chl and decrease of anthocyanin contents in leaves was observed simultaneously. On 28th day, the maximum content of Chl and minimum content of anthocyanins was measured. However, the maximal photosynthetic performance was found one week later, when the content of Chl started to diminish. Our study proved that the achievement of maximal photosynthetic performance of each leaf took about quarter of organ life and this state lasted very shortly. In this work, we described and discussed for the first time the dynamics of Chl, anthocyanins, and flavonols combined with photosynthetic efficiency changes during the leaf life in situ. © The authors.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85076778671,

title = {Genetic and physiological dissection of photosynthesis in barley exposed to drought stress},

author = { A. Daszkowska-Golec and A. Collin and K. Sitko and A. Janiak and H.M. Kalaji and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076778671&doi=10.3390%2fijms20246341&partnerID=40&md5=14faebbe60c567c85947758707f74195},

doi = {10.3390/ijms20246341},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {24},

publisher = {MDPI AG},

abstract = {Balanced photosynthesis under drought is essential for better survival and for agricultural benefits in terms of biomass and yield. Given the current attempts to improve the photosynthetic efficiency for greater crop yield, the explanation of the genetic basis of that process, together with the phenotypic analysis, is significant in terms of both basic studies and potential agricultural application. Therefore, the main objective of this study was to uncover the molecular basis of the photosynthesis process under drought stress in barley. To address that goal, we conducted transcriptomic examination together with detailed photosynthesis analysis using the JIP-test. Using this approach, we indicated that photosynthesis is a process that is very early affected in barley seedlings treated with severe drought stress. Rather than focusing on individual genes, our strategy was pointed to the identification of groups of genes with similar expression patterns. As such, we identified and annotated almost 150 barley genes as crucial core-components of photosystems, electron transport components, and Calvin cycle enzymes. Moreover, we designated 17 possible regulatory interactions between photosynthesis-related genes and transcription factors in barley. Summarizing, our results provide a list of candidate genes for future genetic research and improvement of barley drought tolerance by targeting photosynthesis. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85072909603,

title = {Influence of short-term macronutrient deprivation in maize on photosynthetic characteristics, transpiration and pigment content},

author = { K. Sitko and Ż. Gieroń and M. Szopiński and P. Zieleźnik-Rusinowska and S. Rusinowski and M. Pogrzeba and A. Daszkowska-Golec and H.M. Kalaji and E. Małkowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072909603&doi=10.1038%2fs41598-019-50579-1&partnerID=40&md5=19c9ddd1e85d50ea693ffe9056d05506},

doi = {10.1038/s41598-019-50579-1},

issn = {20452322},

year  = {2019},

date = {2019-01-01},

journal = {Scientific Reports},

volume = {9},

number = {1},

publisher = {Nature Publishing Group},

abstract = {The aim of the research was to compare the impact of short-term deprivation of selected macronutrients (Ca; K; Mg and P) on the photosynthetic characteristics, transpiration and pigment content in maize. The strongest inhibition of photosynthesis was caused by a deprivation of Mg, which was visible as a decrease in the photosynthetic and transpiration rates, stomatal conductance, photosystem II (PSII) performance, chlorophyll and flavonol content with a simultaneously increased content of anthocyanins. In the K-deprived plants, a decrease in the photosynthetic rate was observed. However, the transpiration rate and stomatal conductance did not differ significantly compared with the control. In the K-deprived plants, a decrease in chlorophyll and an increase in the anthocyanin content were also observed. We showed that Ca starvation resulted in a decrease in the photosynthetic and transpiration rates, stomatal conductance and PSII performance, while the pigment content was not significantly different compared with the control. In the case of P-deprived plants, we observed a decrease in the photosynthetic and transpiration rates. Interestingly, the inhibition of stomatal conductance was the strongest in the P-deprived plants compared with all of the investigated elements. However, the performance of PSII was not significantly affected by P starvation compared with the control. Our results present for the first time a comprehensive analysis of the effect of short-term macronutrient deprivation on photosynthesis and transpiration in maize plants. © 2019, The Author(s).},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85040325592,

title = {Prompt chlorophyll fluorescence as a tool for crop phenotyping: an example of barley landraces exposed to various abiotic stress factors},

author = { H.M. Kalaji and A. Rastogi and M. Živčák and M. Brestic and A. Daszkowska-Golec and K. Sitko and K.Y. Alsharafa and R. Lotfi and P. Stypiński and I.A. Samborska and M.D. Cetner},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040325592&doi=10.1007%2fs11099-018-0766-z&partnerID=40&md5=82ab27144de33dedba832444496b1909},

doi = {10.1007/s11099-018-0766-z},

issn = {03003604},

year  = {2018},

date = {2018-01-01},

journal = {Photosynthetica},

volume = {56},

number = {3},

pages = {953-961},

publisher = {The Institute of Experimental Biology of the Czech Academy of Sciences},

abstract = {The study examined photosynthetic efficiency of two barley landraces (cvs. Arabi Abiad and Arabi Aswad) through a prompt fluorescence technique under influence of 14 different abiotic stress factors. The difference in the behavior of photosynthetic parameters under the same stress factor in–between cv. Arabi Abiad and cv. Arabi Aswad indicated different mechanisms of tolerance and strategies for the conversion of light energy into chemical energy for both the landraces. This study confirmed the suitability of some chlorophyll fluorescence parameters as reliable biomarkers for screening the plants at the level of photosynthetic apparatus. © 2018, The Author(s).},

note = {122},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85040322145,

title = {Mutation in barley ERA1 (Enhanced Response to ABA1) gene confers better photosynthesis efficiency in response to drought as revealed by transcriptomic and physiological analysis},

author = { A. Daszkowska-Golec and A. Collin and K. Sitko and M. Słota and M.M. Kurowska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040322145&doi=10.1016%2fj.envexpbot.2018.01.003&partnerID=40&md5=0007d532bbcbed5056c705585b2fd044},

doi = {10.1016/j.envexpbot.2018.01.003},

issn = {00988472},

year  = {2018},

date = {2018-01-01},

journal = {Environmental and Experimental Botany},

volume = {148},

pages = {12-26},

publisher = {Elsevier B.V.},

abstract = {Farnesylation is a post-translational modification that promotes the interaction between the modified signaling protein and membrane lipids and/or other proteins. Farnesyltransferase is the crucial enzyme involved in this process. Strikingly, plant mutants in the ERA1 (Enhanced response to ABA 1) gene, encoding β-subunit of farnesyltransferase, exhibited ABA-hypersensitivity during seed germination and drought tolerance in several species including Arabidopsis, wheat and soybean. However, the mechanism of ERA1 action has not been resolved yet. Here, we present the potential regulatory role of ERA1 in the drought signaling network in barley. With the aim of decoding the role of the ERA1 gene, we developed a unique barley mutant using TILLING analysis. Mutation in HvERA1 confers semi-dwarf phenotype, ABA-sensitivity during seed germination and drought tolerance. Our transcriptomic analysis suggested a role of HvERA1 in regulation of the crosstalk between ABA and ethylene at the onset of drought. Furthermore, analysis of hvera1.b response to prolonged drought stress linked HvERA1 to the metabolism of galactolipids, that build the chloroplast membranes. It might results in the protection of hvera1.b photosystem and thus, in its better photosynthesis performance under water stress. Together, these results indicate the possible mechanism of the primary cause of the observed alterations in the hvera1.b mutant. © 2018 Elsevier B.V.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85043326007,

title = {HorTILLUS—a rich and renewable source of induced mutations for forward/reverse genetics and pre-breeding programs in barley (Hordeumvulgare L.)},

author = { M.E. Szurman-Zubrzycka and J. Zbieszczyk and M. Marzec and J. Jelonek and B. Chmielewska and M.M. Kurowska and M. Krok and A. Daszkowska-Golec and J. Guzy-Wróbelska and D. Gruszka and M. Gajecka and P. Gajewska and M. Stolarek and P. Tylec and P. Sega and S. Lip and M. Kudełko and M. Lorek and M. Gorniak-Walas and A. Malolepszy and N. Podsiadlo and K.P. Szyrajew and A. Keisa and Z. Mbambo and E. Todorowska and M. Gaj and Z. Nita and W. Orlowska-Job and M. Maluszynski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85043326007&doi=10.3389%2ffpls.2018.00216&partnerID=40&md5=2be0ee225baa473124ed786053f7b1c1},

doi = {10.3389/fpls.2018.00216},

issn = {1664462X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Plant Science},

volume = {9},

publisher = {Frontiers Media S.A.},

abstract = {TILLING (Targeting Induced Local Lesions IN Genomes) is a strategy used for functional analysis of genes that combines the classical mutagenesis and a rapid, high-throughput identification of mutations within a gene of interest. TILLING has been initially developed as a discovery platform for functional genomics, but soon it has become a valuable tool in development of desired alleles for crop breeding, alternative to transgenic approach. Here we present the HorTILLUS (Hordeum—TILLING—University of Silesia) population created for spring barley cultivar “Sebastian” after double-treatment of seeds with two chemical mutagens: sodium azide (NaN3) and N-methyl-N-nitrosourea (MNU). The population comprises more than 9,600 M2 plants from which DNA was isolated, seeds harvested, vacuum-packed, and deposited in seed bank. M3 progeny of 3,481 M2 individuals was grown in the field and phenotyped. The screening for mutations was performed for 32 genes related to different aspects of plant growth and development. For each gene fragment, 3,072–6,912 M2 plants were used for mutation identification using LI-COR sequencer. In total, 382 mutations were found in 182.2Mb screened. The average mutation density in the HorTILLUS, estimated as 1 mutation per 477kb, is among the highest mutation densities reported for barley. The majority of mutations were G/C to A/T transitions, however about 8% transversions were also detected. Sixty-one percent of mutations found in coding regions were missense, 37.5% silent and 1.1% nonsense. In each gene, the missense mutations with a potential effect on protein function were identified. The HorTILLUS platform is the largest of the TILLING populations reported for barley and best characterized. The population proved to be a useful tool, both in functional genomic studies and in forward selection of barley mutants with required phenotypic changes. We are constantly renewing the HorTILLUS population, which makes it a permanent source of new mutations. We offer the usage of this valuable resource to the interested barley researchers on cooperative basis. © 2018 Szurman-Zubrzycka, Zbieszczyk, Marzec, Jelonek, Chmielewska, Kurowska, Krok, Daszkowska-Golec, Guzy-Wrobelska, Gruszka, Gajecka, Gajewska, Stolarek, Tylec, Sega, Lip, Kudełko, Lorek, Gorniak-Walas, Malolepszy, Podsiadlo, Szyrajew, Keisa, Mbambo, Todorowska, Gaj, Nita, Orlowska-Job, Maluszynski and Szarejko.},

note = {30},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85040689900,

title = {Emerging roles of the nuclear cap-binding complex in abiotic stress responses},

author = { A. Daszkowska-Golec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040689900&doi=10.1104%2fpp.17.01017&partnerID=40&md5=4cc379a91a43a2bc52d78035a5b8ef3d},

doi = {10.1104/pp.17.01017},

issn = {00320889},

year  = {2018},

date = {2018-01-01},

journal = {Plant Physiology},

volume = {176},

number = {1},

pages = {242-253},

publisher = {American Society of Plant Biologists},

abstract = {[No abstract available]},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021152716,

title = {Mutation in HvCBP20 (Cap binding protein 20) adapts barley to drought stress at phenotypic and transcriptomic levels},

author = { A. Daszkowska-Golec and A. Collin and M. Marzec and M. Słota and M.M. Kurowska and M. Gajecka and P. Gajewska and T. Płociniczak and K. Sitko and A. Pacak and Z. Szweykowska-Kulinska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021152716&doi=10.3389%2ffpls.2017.00942&partnerID=40&md5=769fb24d1f52b6fc895f399773d7ea69},

doi = {10.3389/fpls.2017.00942},

issn = {1664462X},

year  = {2017},

date = {2017-01-01},

journal = {Frontiers in Plant Science},

volume = {8},

publisher = {Frontiers Media S.A.},

abstract = {CBP20 (Cap-Binding Protein 20) encodes a small subunit of the cap-binding complex (CBC), which is involved in the conserved cell processes related to RNA metabolism in plants and, simultaneously, engaged in the signaling network of drought response, which is dependent on ABA. Here, we report the enhanced tolerance to drought stress of barley mutant in the HvCBP20 gene manifested at the morphological, physiological, and transcriptomic levels. Physiological analyses revealed differences between the hvcbp20.ab mutant and its WT in response to a water deficiency. The mutant exhibited a higher relative water content (RWC), a lower stomatal conductance and changed epidermal pattern compared to the WT after drought stress. Transcriptome analysis using the Agilent Barley Microarray integrated with observed phenotypic traits allowed to conclude that the hvcbp20.ab mutant exhibited better fitness to stress conditions by its much more efficient and earlier activation of stress-preventing mechanisms. The network hubs involved in the adjustment of hvcbp20.ab mutant to the drought conditions were proposed. These results enabled to make a significant progress in understanding the role of CBP20 in the drought stress response. © 2017 Daszkowska-Golec, Skubacz, Marzec, Slota, Kurowska, Gajecka, Gajewska, Płociniczak, Sitko, Pacak, Szweykowska-Kulinska and Szarejko.},

note = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85007338538,

title = {The role and regulation of ABI5 (ABA-insensitive 5) in plant development, abiotic stress responses and phytohormone crosstalk},

author = { A. Collin and A. Daszkowska-Golec and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85007338538&doi=10.3389%2ffpls.2016.01884&partnerID=40&md5=a947006f38a2703ae6a7bbfb624d4ed2},

doi = {10.3389/fpls.2016.01884},

issn = {1664462X},

year  = {2016},

date = {2016-01-01},

journal = {Frontiers in Plant Science},

volume = {7},

number = {DECEMBER2016},

publisher = {Frontiers Media S.A.},

abstract = {ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth in the presence of ABA and abiotic stresses. ABI5 functions in the core ABA signaling, which is composed of PYR/PYL/RCAR receptors, PP2C phosphatases and SnRK2 kinases, through the regulation of the expression of genes that contain the ABSCISIC ACID RESPONSE ELEMENT (ABRE) motif within their promoter region. The regulated targets include stress adaptation genes, e.g., LEA proteins. However, the expression and activation of ABI5 is not only dependent on the core ABA signaling. Many transcription factors such as ABI3, ABI4, MYB7 and WRKYs play either a positive or a negative role in the regulation of ABI5 expression. Additionally, the stability and activity of ABI5 are also regulated by other proteins through post-translational modifications such as phosphorylation, ubiquitination, sumoylation and S-nitrosylation. Moreover, ABI5 also acts as an ABA and other phytohormone signaling integrator. Components of auxin, cytokinin, gibberellic acid, jasmonate and brassinosteroid signaling and metabolism pathways were shown to take part in ABI5 regulation and/or to be regulated by ABI5. Monocot orthologs of AtABI5 have been identified. Although their roles in the molecular and physiological adaptations during abiotic stress have been elucidated, knowledge about their detailed action still remains elusive. Here, we describe the recent advances in understanding the action of ABI5 in early developmental processes and the adaptation of plants to unfavorable environmental conditions. We also focus on ABI5 relation to other phytohormones in the abiotic stress response of plants. © 2016 Skubacz, Daszkowska-Golec and Szarejko.},

note = {216},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84960405354,

title = {Transcriptome analysis reveals the role of the root hairs as environmental sensors to maintain plant functions under water-deficiency conditions},

author = { M. Kwaśniewski and A. Daszkowska-Golec and A. Janiak and K. Chwiałkowska and U. Nowakowska and G. Sablok and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84960405354&doi=10.1093%2fjxb%2ferv498&partnerID=40&md5=32765e7f173f0f3dbd3b50cefecd7d96},

doi = {10.1093/jxb/erv498},

issn = {00220957},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Experimental Botany},

volume = {67},

number = {4},

pages = {1079-1094},

publisher = {Oxford University Press},

abstract = {An important part of the root system is the root hairs, which play a role in mineral and water uptake. Here, we present an analysis of the transcriptomic response to water deficiency of the wild-Type (WT) barley cultivar 'Karat' and its root-hairless mutant rhl1.a. A comparison of the transcriptional changes induced by water stress resulted in the identification of genes whose expression was specifically affected in each genotype. At the onset of water stress, more genes were modulated by water shortage in the roots of the WT plants than in the roots of rhl1.a. The roots of the WT plants, but not of rhl1.a, specifically responded with the induction of genes that are related to the abscisic acid biosynthesis, stomatal closure, and cell wall biogenesis, thus indicating the specific activation of processes that are related to water-stress signalling and protection. On the other hand, the processes involved in the further response to abiotic stimuli, including hydrogen peroxide, heat, and high light intensity, were specifically up-regulated in the leaves of rhl1.a. An extended period of severe stress caused more drastic transcriptome changes in the roots and leaves of the rhl1.a mutant than in those of the WT. These results are in agreement with the much stronger damage to photosystem II in the rhl1.a mutant than in its parent cultivar after 10 d of water stress. Taking into account the putative stress sensing and signalling features of the root hair transcriptome, we discuss the role of root hairs as sensors of environmental conditions. © 2015 The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.},

note = {50},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84890362315,

title = {Open or close the gate - Stomata action under the control of phytohormones in drought stress conditions},

author = { A. Daszkowska-Golec and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84890362315&doi=10.3389%2ffpls.2013.00138&partnerID=40&md5=7bbcd6583dfe00ef4bb2cc5d6aaa8b63},

doi = {10.3389/fpls.2013.00138},

issn = {1664462X},

year  = {2013},

date = {2013-01-01},

journal = {Frontiers in Plant Science},

volume = {4},

number = {MAY},

publisher = {Frontiers Research Foundation},

abstract = {Two highly specialized cells, the guard cells that surround the stomatal pore, are able to integrate environmental and endogenous signals in order to control the stomatal aperture and thereby the gas exchange. The uptake of CO2 is associated with a loss of water by leaves. Control of the size of the stomatal aperture optimizes the efficiency of water use through dynamic changes in the turgor of the guard cells. The opening and closing of stomata is regulated by the integration of environmental signals and endogenous hormonal stimuli. The various different factors to which the guard cells respond translates into the complexity of the network of signaling pathways that control stomatal movements. The perception of an abiotic stress triggers the activation of signal transduction cascades that interact with or are activated by phytohormones. Among these, abscisic acid (ABA), is the best-known stress hormone that closes the stomata, although other phytohormones, such as jasmonic acid, brassinosteroids, cytokinins, or ethylene are also involved in the stomatal response to stresses. As a part of the drought response, ABA may interact with jasmonic acid and nitric oxide in order to stimulate stomatal closure. In addition, the regulation of gene expression in response to ABA involves genes that are related to ethylene, cytokinins, and auxin signaling. In this paper, recent findings on phytohormone crosstalk, changes in signaling pathways including the expression of specific genes and their impact on modulating stress response through the closing or opening of stomata, together with the highlights of gaps that need to be elucidated in the signaling network of stomatal regulation, are reviewed. © 2013 Daszkowska-Golec and Szarejko.},

note = {333},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85003413378,

title = {Towards the identification of new genes involved in ABA-dependent abiotic stresses using Arabidopsis suppressor mutants of abh1 hypersensitivity to ABA during seed germination},

author = { A. Daszkowska-Golec and E. Chorazy and M. Maluszynski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85003413378&doi=10.3390%2fijms140713403&partnerID=40&md5=952cad5d4d745f835c6effceaf399df8},

doi = {10.3390/ijms140713403},

issn = {14220067},

year  = {2013},

date = {2013-01-01},

journal = {International journal of molecular sciences},

volume = {14},

number = {7},

pages = {13403-13432},

abstract = {Abscisic acid plays a pivotal role in the abiotic stress response in plants. Although great progress has been achieved explaining the complexity of the stress and ABA signaling cascade, there are still many questions to answer. Mutants are a valuable tool in the identification of new genes or new alleles of already known genes and in elucidating their role in signaling pathways. We applied a suppressor mutation approach in order to find new components of ABA and abiotic stress signaling in Arabidopsis. Using the abh1 (ABA hypersensitive 1) insertional mutant as a parental line for EMS mutagenesis, we selected several mutants with suppressed hypersensitivity to ABA during seed germination. Here, we present the response to ABA and a wide range of abiotic stresses during the seed germination and young seedling development of two suppressor mutants-soa2 (suppressor of abh1 hypersensitivity to ABA 2) and soa3 (suppressor of abh1 hypersensitivity to ABA 3). Generally, both mutants displayed a suppression of the hypersensitivity of abh1 to ABA, NaCl and mannitol during germination. Both mutants showed a higher level of tolerance than Columbia-0 (Col-0-the parental line of abh1) in high concentrations of glucose. Additionally, soa2 exhibited better root growth than Col-0 in the presence of high ABA concentrations. soa2 and soa3 were drought tolerant and both had about 50% fewer stomata per mm2 than the wild-type but the same number as their parental line-abh1. Taking into account that suppressor mutants had the same genetic background as their parental line-abh1, it was necessary to backcross abh1 with Landsberg erecta four times for the map-based cloning approach. Mapping populations, derived from the cross of abh1 in the Landsberg erecta background with each suppressor mutant, were created. Map based cloning in order to identify the suppressor genes is in progress.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84871422647,

title = {Arabidopsis suppressor mutant of abh1 shows a new face of the already known players: ABH1 (CBP80) and ABI4-in response to ABA and abiotic stresses during seed germination},

author = { A. Daszkowska-Golec and W. Wojnar and M. Rosikiewicz and I. Szarejko and M. Maluszynski and Z. Szweykowska-Kulinska and A. Jarmolowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84871422647&doi=10.1007%2fs11103-012-9991-1&partnerID=40&md5=7f81de2fc3671e5dfd0a59c19ed1cfec},

doi = {10.1007/s11103-012-9991-1},

issn = {01674412},

year  = {2013},

date = {2013-01-01},

journal = {Plant Molecular Biology},

volume = {81},

number = {1-2},

pages = {189-209},

publisher = {Kluwer Academic Publishers},

abstract = {Although the importance of abscisic acid (ABA) in plant development and response to abiotic and biotic stresses is well recognized, the molecular basis of the signaling pathway has not been fully elucidated. Mutants in genes related to ABA are widely used as a tool for gaining insight into the mechanisms of ABA signal transduction and ABA-dependent stress response. We used a genetic approach of a suppressor screening in order to decipher the interaction between ABH1 (CBP80) and other components of ABA signaling. ABH1 (CBP80) encodes a large subunit of CBC (CAP BINDING COMPLEX) and the abh1 mutant is drought-tolerant and hypersensitive to ABA during seed germination. The suppressor mutants of abh1 were generated after chemical mutagenesis. The mutant named soa1 (suppressor of abh1 hypersensitivity to ABA 1) displayed an ABA-insensitive phenotype during seed germination. The genetic analysis showed that the soa1 phenotype is dominant in relation to abh1 and segregates as a single locus. Based on soa1's response to a wide spectrum of physiological assays during different stages of development, we used the candidate-genes approach in order to identify a suppressor gene. The molecular analysis revealed that mutation causing the phenotype of soa1 occurred in the ABI4 (ABA insensitive 4) gene. Analysis of pre-miR159 expression, whose processing depends on CBC, as well as targets of miR159: MYB33 and MYB101, which are positive regulators of ABA signaling, revealed a possible link between CBP80 (ABH1) and ABI4 presented here. © 2012 The Author(s).},

note = {24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-82355175692,

title = {Arabidopsis seed germination under abiotic stress as a concert of action of phytohormones},

author = { A. Daszkowska-Golec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-82355175692&doi=10.1089%2fomi.2011.0082&partnerID=40&md5=8a9f0ccf88e3a84217c40284b73b3709},

doi = {10.1089/omi.2011.0082},

issn = {15362310},

year  = {2011},

date = {2011-01-01},

journal = {OMICS A Journal of Integrative Biology},

volume = {15},

number = {11},

pages = {763-774},

abstract = {Different abiotic stresses inhibit or delay the development and growth of plants. The most crucial step of plant life cycle, which ensures the survival of the next generation, is seed germination. Plants are sessile organisms that need to integrate internal and external signals in order to produce the correct response. Plants have evolved mechanisms that enable seed germination to be arrested under stress conditions and then resumed when conditions are favorable. The complexity of this mechanism was explored in Arabidopsis thaliana using mutants that had defects in their phytohormone metabolism and signaling pathways. These analyses led to the identification of many important components that are involved in these pathways and shed light on the complex crosstalk between phytohormones under abiotic stress. Combined "omics" techniques such as functional genomics, transcriptomics, and proteomics with the support of bioinformatics, physiology, and molecular genetics have greatly expanded the present understanding of the seed germination process. This minireview focuses on the current status of knowledge about seed germination under abiotic stress with a particular emphasis on genetic interactions, hormonal balance, and epigenetic regulation that occur in Arabidopsis thaliana during this process. © Copyright 2011, Mary Ann Liebert, Inc.},

note = {46},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-80053627631,

title = {TILLING - a shortcut in functional genomics},

author = { M.M. Kurowska and A. Daszkowska-Golec and D. Gruszka and M. Marzec and M. Szurman and I. Szarejko and M. Maluszynski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80053627631&doi=10.1007%2fs13353-011-0061-1&partnerID=40&md5=59e48b59a6de3676dbabe53974f2599e},

doi = {10.1007/s13353-011-0061-1},

issn = {12341983},

year  = {2011},

date = {2011-01-01},

journal = {Journal of Applied Genetics},

volume = {52},

number = {4},

pages = {371-390},

abstract = {Recent advances in large-scale genome sequencing projects have opened up new possibilities for the application of conventional mutation techniques in not only forward but also reverse genetics strategies. TILLING (Targeting Induced Local Lesions IN Genomes) was developed a decade ago as an alternative to insertional mutagenesis. It takes advantage of classical mutagenesis, sequence availability and high-throughput screening for nucleotide polymorphisms in a targeted sequence. The main advantage of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of its genome size and ploidy level. The TILLING protocol provides a high frequency of point mutations distributed randomly in the genome. The great mutagenic potential of chemical agents to generate a high rate of nucleotide substitutions has been proven by the high density of mutations reported for TILLING populations in various plant species. For most of them, the analysis of several genes revealed 1 mutation/200-500 kb screened and much higher densities were observed for polyploid species, such as wheat. High-throughput TILLING permits the rapid and low-cost discovery of new alleles that are induced in plants. Several research centres have established a TILLING public service for various plant species. The recent trends in TILLING procedures rely on the diversification of bioinformatic tools, new methods of mutation detection, including mismatch-specific and sensitive endonucleases, but also various alternatives for LI-COR screening and single nucleotide polymorphism (SNP) discovery using next-generation sequencing technologies. The TILLING strategy has found numerous applications in functional genomics. Additionally, wide applications of this throughput method in basic and applied research have already been implemented through modifications of the original TILLING strategy, such as Ecotilling or Deletion TILLING. © 2011 The Author(s).},

note = {130},

keywords = {},

pubstate = {published},

tppubtype = {article}

}