@article{2-s2.0-85182572811,

title = {Toxic effect of mercury on arbuscular mycorrhizal fungi colonisation and physiological status of three seed-based Miscanthus hybrids},

author = { A. Szada-Borzyszkowska and J. Krzyżak and S. Rusinowski and K. Sitko and M. Pogrzeba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85182572811&doi=10.1016%2fj.jtemb.2024.127391&partnerID=40&md5=7a96c20e94ff0ada25689a6332a66cdd},

doi = {10.1016/j.jtemb.2024.127391},

issn = {0946672X},

year  = {2024},

date = {2024-01-01},

journal = {Journal of Trace Elements in Medicine and Biology},

volume = {83},

publisher = {Elsevier GmbH},

abstract = {Background: Currently, mercury pollution is a widespread problem in the world. As mercury is difficult to remove from the environment, it has long-term negative effects on soil health and human life. One of the techniques to stabilise Hg is phytostabilisation, which can be supported by arbuscular mycorrhizal fungi (AMF). Methods: In a 4-month pot experiment, we investigated the suitability of three seed-based Miscanthus hybrids (GNT3; GNT34; GNT43) for growth on soils heavily polluted with mercury (6795.7 mg kg-1). During the experiment, the effects of high soil contamination with mercury on physiological parameters and colonisation of roots of seed-based Miscanthus hybrids by indigenous AMF from Hg-contaminated and uncontaminated soils were investigated. Results: A high pseudo-total Hg concentration (6795.75 mg kg-1) in soil was found. The Hg content in the aerial part of GNT34 grown on Hg-contaminated soil was 1.5 times and 3 times higher than GNT3 and GNT43, respectively. The Hg content in the roots of GNT3 on Hg-contaminated soil was 25% and 10% lower than that of GNT34 and GNT43, respectively. The N content in the aboveground part of GNT34 in the Hg variant was 13.5% lower compared to the control soil. The P and K content in the shoots of the Miscanthus hybrids was lower in the plants grown on Hg-contaminated soil. The P content in GNT43 in the Hg variant was 33% and 19% lower than in GNT34 and GNT3, respectively. The K content in GNT34 in the Hg variant was 24.7% and 31.4% higher than in GNT43 and GNT3, respectively. The dry weight of the shoots and roots as well as the shoot height of the Miscanthus hybrids were lower in Hg-contaminated soil. Lower values of AMF root colonisation parameters (F; M) were observed in the plants in the Hg variant. In the Hg variant, a lower photosynthetic rate and a decrease in chlorophyll content were observed in the leaves of the Miscanthus hybrids. In the Hg variant, an increase in the content of flavonols was observed. The strongest toxic effect of mercury on the light phase of photosynthesis was measured in GNT34. Conclusion: Soils heavily contaminated with mercury negatively affected the physiological parameters of Miscanthus, as evidenced by a decrease in photosynthetic rate and biomass. The ability of indigenous AMF from Hg-contaminated soils to colonise the roots of seed-based Miscanthus hybrids was limited. © 2024 Elsevier GmbH},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85163677050,

title = {The effect of combined drought and trace metal elements stress on the physiological response of three Miscanthus hybrids},

author = { J. Krzyżak and S. Rusinowski and K. Sitko and A. Szada-Borzyszkowska and R. Stec and P. Janota and E. Jensen and A. Kiesel and M. Pogrzeba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85163677050&doi=10.1038%2fs41598-023-37564-5&partnerID=40&md5=4b934e9754d61364f12d8fce4b09399f},

doi = {10.1038/s41598-023-37564-5},

issn = {20452322},

year  = {2023},

date = {2023-01-01},

journal = {Scientific Reports},

volume = {13},

number = {1},

publisher = {Nature Research},

abstract = {Drought is a serious threat worldwide and has a significant impact on agricultural production and soil health. It can pose an even greater threat when it involves land contaminated with trace metal element (TMEs). To prevent desertification, such land should be properly managed and growing Miscanthus for energy or raw material purposes could be a solution. The effects of drought and TMEs were studied in a pot experiment on three different Miscanthus hybrids (conventional Miscanthus × giganteus; TV1 and GNT10) considering growth parameters, photosynthetic parameters and elemental composition of roots, rhizomes and shoots. GNT10 was characterised by the weakest gas exchange among the hybrids, which was compensated by the highest number of leaves and biomass. The strongest correlations between the studied parameters were found for TV1, which might indicate a high sensitivity to TME stress. For M × g and GNT10, the main mechanisms for coping with stress seem to be biomass management through number of shoots and leaves and gas exchange. The main factor determining the extent of accumulation of TMEs was the amount of water applied in the experimental treatment, which was related to the location of the plant in the aniso-isohydric continuum. GNT10 was the most resistant plant to combined stress, while it responded similarly to TV1 when drought and trace metal elements were applied separately. © 2023, The Author(s).},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85160530058,

title = {The Effect of Combined Drought and Temperature Stress on the Physiological Status of Calcareous Grassland Species as Potential Candidates for Urban Green Infrastructure},

author = { J. Krzyżak and S. Rusinowski and K. Sitko and A. Szada-Borzyszkowska and J. Borgulat and R. Stec and H.M. Hanslin and M. Pogrzeba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160530058&doi=10.3390%2fplants12102003&partnerID=40&md5=d3d86cb625088ab2c969a47a9d4ab7b3},

doi = {10.3390/plants12102003},

issn = {22237747},

year  = {2023},

date = {2023-01-01},

journal = {Plants},

volume = {12},

number = {10},

publisher = {MDPI},

abstract = {Nature-based solutions are promising for climate adaptation and environmental management in urban areas, but urban conditions are stressful for vegetation. In particular, the interaction of drought and high temperatures may be detrimental. Guiding plant selection for urban greening with native species requires a far better knowledge of plant adaptations and stress acclimation. We tested the physiological responses of four candidate calcareous grassland species for green roofs and walls to the combined effects of drought and high temperatures under controlled conditions. The tested species proved relatively resistant to stress despite different strategies to protect the photosynthetic apparatus, maintain water balance, and repair damages. Based on the physiological responses, we rank the species in descending order of resistance to the stress factors tested: Trifolium medium > Festuca ovina > Carex flacca > Potentilla reptans, but all four can serve as potential candidates for green walls and roofs. Physiological stress screening of plant species for use on green roofs and walls supplements the habitat template approach to provide a stronger and wider base for prioritizations. © 2023 by the authors.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85145859892,

title = {The Effect of Different Agrotechnical Treatments on the Establishment of Miscanthus Hybrids in Soil Contaminated with Trace Metals},

author = { J. Krzyżak and S. Rusinowski and K. Sitko and A. Szada-Borzyszkowska and R. Stec and E. Jensen and J. Clifton-Brown and A. Kiesel and E.F. Lewin and P. Janota and M. Pogrzeba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145859892&doi=10.3390%2fplants12010098&partnerID=40&md5=25238aa3a301ad08f4053947674e433f},

doi = {10.3390/plants12010098},

issn = {22237747},

year  = {2023},

date = {2023-01-01},

journal = {Plants},

volume = {12},

number = {1},

publisher = {MDPI},

abstract = {Climate change and man-made pollution can have a negative impact on the establishment of Miscanthus plants in the field. This is particularly important because biomass can be produced on marginal land without conflicting with food crops. The establishment success depends on the hybrid chosen, the cultivation method, the climatic conditions, and the concentration of pollutants in the soil. There are several ways to increase the survival rate of the plants during the first growing season and after the first winter. One of them is the application of biochar and photodegradable plastic mulch, which can provide a solution for soils polluted with trace elements (TMEs). The aim of this study was to investigate the application of plastic mulch and biochar separately and in combination at the planting stage for two Miscanthus hybrids planted by the rhizome method (TV1) and seedling plugs (GNT43) on soils contaminated with trace metal elements (Pb; Cd; Zn). TV1 seems unsuitable for TME-contaminated field cultivation, as the survival rate was <60% in most treatments studied. The selected treatments did not increase the survival rate. Furthermore, the application of plastic mulch in combination with biochar resulted in a significant reduction of this parameter, regardless of the hybrid studied. The applied agrotechnology did not influence the TME accumulation in the aboveground plant parts in TV1, while Pb and Cd in GNT43 showed significantly higher values in all treatments. Contrary to expectations, biochar and plastic mulch applied separately and together neither increased survival nor reduced the accumulation of toxic TMEs during establishment on soil contaminated with TMEs and after the first growing season. © 2022 by the authors.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85129120912,

title = {Field Evaluation of Arbuscular Mycorrhizal Fungal Colonization in Miscanthus × giganteus and Seed-Based Miscanthus Hybrids Grown in Heavy-Metal-Polluted Areas},

author = { A. Szada-Borzyszkowska and J. Krzyżak and S. Rusinowski and K. Sitko and M. Pogrzeba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85129120912&doi=10.3390%2fplants11091216&partnerID=40&md5=205e7e1d7da07dcd9df2b82621bda9a5},

doi = {10.3390/plants11091216},

issn = {22237747},

year  = {2022},

date = {2022-01-01},

journal = {Plants},

volume = {11},

number = {9},

publisher = {MDPI},

abstract = {Understanding the behavior of arbuscular mycorrhizal fungi (AMF) associated with plants is essential for optimizing plant cultivation to the phytoremediation of degraded soils. The objective of the present study was to evaluate the differences in AMF root colonization between novel seed-based interspecific Miscanthus hybrids (M. sacchariflorus × M. sinensis) and the standard M. × giganteus when grown in soils contaminated with heavy metals (Pb; Cd; and Zn). During the third and fourth growing seasons, higher levels of metals in the roots and a reduced transfer of metals from the roots to the shoots were observed in all the plants studied. After the third growing season, GNT5 had the highest AMF root colonization rates in soil contaminated with heavy metals. The lowest AMF colonization rates were observed for the GNT34 hybrid. After the fourth growing season, AMF colonization decreased, which could be due to the drought that occurred during that season. GNT34 showed a lower tendency to develop mycorrhizal structures on heavy-metal (HM)-contaminated soils than GNT41 and M × g; however, this cultivar was insensitive to changes in colonization rates during the dry growing season. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85121653518,

title = {Effect of drought and heavy metal contamination on growth and photosynthesis of silver birch trees growing on post-industrial heaps},

author = { K. Sitko and M. Opała-Owczarek and G. Jemioła and Ż. Gieroń and M. Szopiński and P. Owczarek and M. Rudnicka and E. Małkowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121653518&doi=10.3390%2fcells11010053&partnerID=40&md5=49e0f5180a420dc9454161db9382a90e},

doi = {10.3390/cells11010053},

issn = {20734409},

year  = {2022},

date = {2022-01-01},

journal = {Cells},

volume = {11},

number = {1},

publisher = {MDPI},

abstract = {Silver birch trees (Betula pendula Roth) are a pioneering species in post-industrial habitats, and have been associated with an expansive breeding strategy and low habitat requirements. We conducted ecophysiological and dendroclimatological studies to check whether there are any features of which the modification enables birch trees to colonise extreme habitats successfully. We characterised the efficiency of the photosynthetic apparatus, the gas exchange, the content of pigments in leaves, and the growth (leaf thickness and tree-ring width) of birch trees on a post-coal mine heap, a post-smelter heap, and a reference site. Birch growth was limited mainly by temperature and water availability during summer, and the leaves of the birch growing on postindustrial heaps were significantly thicker than the reference leaves. Moreover, birch trees growing on heaps were characterised by a significantly higher content of flavonols and anthocyanins in leaves and higher non-photochemical quenching. In addition, birches growing on the post-coal mine heap accumulated a concentration of Mn in their leaves, which is highly toxic for most plant species. Increasing the thickness of leaves, and the content of flavonols and anthocyanins, as well as efficient non-photochemical quenching seem to be important features that improve the colonization of extreme habitats by birches. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85104076092,

title = {Physiological status and biomass yield of Sida hermaphrodita (L.) Rusby cultivated on two distinct marginal lands in Southern and Northern Poland},

author = { M. Bury and S. Rusinowski and K. Sitko and J. Krzyżak and T. Kitczak and E. Możdżer and H. Siwek and M. Włodarczyk and P. Zieleźnik-Rusinowska and A. Szada-Borzyszkowska and M. Pogrzeba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104076092&doi=10.1016%2fj.indcrop.2021.113502&partnerID=40&md5=0c896df9d16a2b310be73f514c4ceab2},

doi = {10.1016/j.indcrop.2021.113502},

issn = {09266690},

year  = {2021},

date = {2021-01-01},

journal = {Industrial Crops and Products},

volume = {167},

publisher = {Elsevier B.V.},

abstract = {Under EU legislation, energy crops must be grown on marginal land. Sida hermaphrodita L. Rusby is one of the promising perennial energy crops. The study aimed to investigate the physiological status of S. hermaphrodita mature plantations growing on two different marginal lands - one contaminated with heavy metals (M) and one nutrient-poor sandy soil (NM). On the second marginal land, two different harvesting practices have been carried out since establishment: one harvest (NM1) and two repeated harvests (NM2) of biomass each year before the experiment. Measurements were made of gas exchange, chlorophyll a fluorescence, plant pigment content, canopy architecture, growth parameters, and biomass yield. A higher biomass yield (2-fold) was found on the M site compared with both treatments on the NM site. It could be concluded that soil quality was the main reason for the higher yield at M site. S. hermpaphrodita being a heavy metal excluding plant is a more suitable option for fertile but heavy metal polluted soils than nutrient-poor soils, however, biomass quality should be further investigated. © 2021 Elsevier B.V.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85108896930,

title = {The different faces of arabidopsis arenosa—a plant species for a special purpose},

author = { Ż. Gieroń and K. Sitko and E. Małkowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108896930&doi=10.3390%2fplants10071342&partnerID=40&md5=cf109151e0f2a5f879f7762e931151a3},

doi = {10.3390/plants10071342},

issn = {22237747},

year  = {2021},

date = {2021-01-01},

journal = {Plants},

volume = {10},

number = {7},

publisher = {MDPI AG},

abstract = {The following review article collects information on the plant species Arabidopsis arenosa. Thus far, A. arenosa has been known as a model species for autotetraploidy studies because, apart from diploid individuals, there are also tetraploid populations, which is a unique feature of this Arabidopsis species. In addition, A arenosa has often been reported in heavy metal-contaminated sites, where it occurs together with a closely related species A. halleri, a model plant hyperaccumulator of Cd and Zn. Recent studies have shown that several populations of A. arenosa also exhibit Cd and Zn hyperaccumulation. However, it is assumed that the mechanism of hyperaccumulation differs between these two Arabidopsis species. Nevertheless, this phenomenon is still not fully understood, and thorough research is needed. In this paper, we summarize the current state of knowledge regarding research on A. arenosa. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85099815533,

title = {Ecophysiology of Arabidopsis arenosa, a new hyperaccumulator of Cd and Zn},

author = { Ż. Gieroń and K. Sitko and P. Zieleźnik-Rusinowska and M. Szopiński and M. Rojek-Jelonek and A. Rostański and M. Rudnicka and E. Małkowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099815533&doi=10.1016%2fj.jhazmat.2021.125052&partnerID=40&md5=88d8211a77cf4282548591dddcd9342b},

doi = {10.1016/j.jhazmat.2021.125052},

issn = {03043894},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Hazardous Materials},

volume = {412},

publisher = {Elsevier B.V.},

abstract = {Arabidopsis arenosa is a pseudo-metallophyte, closely related to the model hyperaccumulator of Cd and Zn Arabidopsis halleri. A. arenosa occurs naturally in both diploid (2C) and tetraploid (4C) form, in contrast to A. halleri in which only diploid forms were found. Moreover, A. arenosa similarly to A. halleri often occupies heavy metal (HM) contaminated sites. Nevertheless, knowledge about the ecophysiology of this species is very limited. Therefore, we examined fourteen populations of A. arenosa of different ploidy from Central Europe in situ, focusing on photosynthetic efficiency, pigment content and ability to accumulate selected elements. The presented results indicate that several tetraploid populations exhibit the features of Cd and Zn hyperaccumulation. On the one hand, we noted differences in physiological parameters between the studied populations, on the other, harshness of the environment caused similar physiological response such as high HM pollution. All these features suggest that A. arenosa, especially as a new hyperaccumulator of Cd and Zn and autopolyploidyzation model, may be considered a very interesting research object, particularly when investigating the mechanisms of HMs accumulation and tolerance in plants. © 2021 Elsevier B.V.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85105478282,

title = {Is the age of novel ecosystem the factor driving arbuscular mycorrhizal colonization in poa compressa and calamagrostis epigejos?},

author = { G. Woźniak and D. Chmura and E. Małkowski and P. Zieleźnik-Rusinowska and K. Sitko and B. Ziemer and A. Błońska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105478282&doi=10.3390%2fplants10050949&partnerID=40&md5=27acd0c14d3bf379d322763479c460ef},

doi = {10.3390/plants10050949},

issn = {22237747},

year  = {2021},

date = {2021-01-01},

journal = {Plants},

volume = {10},

number = {5},

publisher = {MDPI AG},

abstract = {Some sites transformed or created by humans (novel ecosystem) are different both in vegetation and ecosystems establishment and development. The unknown habitat conditions and new species composition is resulting in new abiotic and biotic systems. To improve the understanding of the process governing the relationships between the environmental factors, plant species assemblages and their arbuscular mycorrhizal fungi (AMF) inoculation were studied in chronosequence on post-coal mine heaps. We hypothesized that AMF root colonization will be dependent on the age of heap and not on the dominant plant species (vegetation type). The high frequency of mycorrhizal colonization of roots (F%) of Poa compressa-and Calamagrostis epigejos-dominated vegetation type was stated. All mycorrhizal parameters were lower in C. epigejos roots when compared to P. compressa (ranging from 60% to 90%). The highest relative mycorrhizal intensity, M%, and mean abundance of arbuscula, A%, in the roots of both examined plants were recorded in vegetation patches dominated by Daucus carota. Positive and statistically significant correlations were found between F%, M%, and A%, and lack of correlation between the heaps’ age and mycorrhizal parameters, and statistically significant correlations between A% and potassium and magnesium content were revealed. The interspecific relations in the novel ecosystems become more complex along with the increase of diversity. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85092471159,

title = {Different strategies of Cd tolerance and accumulation in Arabidopsis halleri and Arabidopsis arenosa},

author = { M. Szopiński and K. Sitko and S. Rusinowski and P. Zieleźnik-Rusinowska and M. Corso and A. Rostański and M. Rojek-Jelonek and N. Verbruggen and E. Małkowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092471159&doi=10.1111%2fpce.13883&partnerID=40&md5=4f1af6b2114a9cba7a10c73ac3c07316},

doi = {10.1111/pce.13883},

issn = {01407791},

year  = {2020},

date = {2020-01-01},

journal = {Plant Cell and Environment},

volume = {43},

number = {12},

pages = {3002-3019},

publisher = {Blackwell Publishing Ltd},

abstract = {Pseudometallophytes are commonly used to study the evolution of metal tolerance and accumulation traits in plants. Within the Arabidopsis genus, the adaptation of Arabidopsis halleri to metalliferous soils has been widely studied, which is not the case for the closely related species Arabidopsis arenosa. We performed an in-depth physiological comparison between the A. halleri and A. arenosa populations from the same polluted site, together with the geographically close non-metallicolous (NM) populations of both species. The ionomes, growth, photosynthetic parameters and pigment content were characterized in the plants that were growing on their native site and in a hydroponic culture under Cd treatments. In situ, the metallicolous (M) populations of both species hyperaccumulated Cd and Zn. The NM population of A. halleri hyperaccumulated Cd and Zn while the NM A. arenosa did not. In the hydroponic experiments, the NM populations of both species accumulated more Cd in their shoots than the M populations. Our research suggests that the two Arabidopsis species evolved different strategies of adaptation to extreme metallic environments that involve fine regulation of metal homeostasis, adjustment of the photosynthetic apparatus and accumulation of flavonols and anthocyanins. © 2020 John Wiley & Sons Ltd},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85089696682,

title = {Cuticular waxes—A shield of barley mutant in CBP20 (Cap-Binding Protein 20) gene when struggling with drought stress},

author = { A. Daszkowska-Golec and J. Karcz and T. Płociniczak and K. Sitko and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089696682&doi=10.1016%2fj.plantsci.2020.110593&partnerID=40&md5=ad2913c77b8570c0d656e8236defcf56},

doi = {10.1016/j.plantsci.2020.110593},

issn = {01689452},

year  = {2020},

date = {2020-01-01},

journal = {Plant Science},

volume = {300},

publisher = {Elsevier Ireland Ltd},

abstract = {CBP20 (Cap-Binding Protein 20) encodes a small subunit of nuclear Cap-Binding Complex (nCBC) that together with CBP80 binds mRNA cap. We previously described barley hvcbp20.ab mutant that demonstrated higher leaf water content and faster stomatal closure than the WT after drought stress. Hence, we presumed that the better water-saving mechanism in hvcbp20.ab may result from the lower permeability of epidermis that together with stomata action limit the water evaporation under drought stress. We asked whether hvcbp20.ab exhibited any differences in wax load on the leaf surface when subjected to drought in comparison to WT cv. ‘Sebastian’. To address this question, we investigated epicuticular wax structure and chemical composition under drought stress in hvcbp20.ab mutant and its WT. We showed that hvcbp20.ab mutant exhibited the increased deposition of cuticular wax. Moreover, our gene expression results suggested a role of HvCBP20 as a negative regulator of both, the biosynthesis of waxes at the level of alkane-forming, and waxes transportation. Interestingly, we also observed increased wax deposition in Arabidopsis cbp20 mutant exposed to drought, which allowed us to describe the CBP20-regulated epicuticular wax accumulation under drought stress in a wider evolutionarily context. © 2020 Elsevier B.V.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85079467816,

title = {Combined use of companion planting and PGPR for the assisted phytoextraction of trace metals (Zn, Pb, Cd)},

author = { A. Konkolewska and A. Piechalak and L. Ciszewska and N. Antos-Krzemińska and T. Skrzypczak and A. Hanć and K. Sitko and E. Małkowski and D. Barałkiewicz and A. Małecka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079467816&doi=10.1007%2fs11356-020-07885-3&partnerID=40&md5=dcdc64ddf8d94a1600ff9843ccb9b2cf},

doi = {10.1007/s11356-020-07885-3},

issn = {09441344},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Science and Pollution Research},

volume = {27},

number = {12},

pages = {13809-13825},

publisher = {Springer},

abstract = {Biomass production and metal accumulation in plant tissue (bioconcentration) are two critical factors limiting the phytoextraction rate. Metal translocation to aboveground organs should be accounted for as the third most important factor, as harvesting of the plant roots is usually economically disadvantageous. These three parameters could be potentially increased with the use of companion planting, a well-known agricultural technique, and inoculation with plant growth–promoting bacteria (PGPB). The aim of the study was to determine whether intercropping and inoculation with endophytic PGPB (Burkholderia phytofirmans PsJNT) can increase the efficiency of phytoextraction of Zn, Pb, and Cd. The study was conducted on Brassica juncea (L.) Czern. “Małopolska” grown in a monoculture or co-planted with Zea mays L. “Codimon” and Medicago sativa L. “Sanditi.” Results show that companion planting and inoculation with rhizobacteria can increase the efficiency of metal phytoextraction, mainly by increasing the yield of dry biomass and the survival rate of plants grown on contaminated soil. We have shown that the simultaneous planting of B. juncea with M. sativa and inoculation with PGPB were the most efficient variants of assisted phytoextraction reaching a recovery of 95% Zn, 90% Cd, and on average about 160% Pb compared with control B. juncea plants grown in monoculture. © 2020, The Author(s).},

note = {24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85082256652,

title = {Hormesis in plants: The role of oxidative stress, auxins and photosynthesis in corn treated with CD or PB},

author = { E. Małkowski and K. Sitko and M. Szopiński and Ż. Gieroń and M. Pogrzeba and H.M. Kalaji and P. Zieleźnik-Rusinowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85082256652&doi=10.3390%2fijms21062099&partnerID=40&md5=d0da0e2d871a72f588bcd16f20258c75},

doi = {10.3390/ijms21062099},

issn = {16616596},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Molecular Sciences},

volume = {21},

number = {6},

publisher = {MDPI AG},

abstract = {Hormesis, which describes the stimulatory effect of low doses of toxic substances on growth, is a well-known phenomenon in the plant and animal kingdoms. However, the mechanisms that are involved in this phenomenon are still poorly understood. We performed preliminary studies on corn coleoptile sections, which showed a positive correlation between the stimulation of growth by Cd or Pb and an increase in the auxin and H2O2 content in the coleoptile sections. Subsequently, we grew corn seedlings in hydroponic culture and tested a wide range of Cd or Pb concentrations in order to determine hormetic growth stimulation. In these seedlings the gas exchange and the chlorophyll a fluorescence, as well as the content of chlorophyll, flavonol, auxin and hydrogen peroxide, were measured. We found that during the hormetic stimulation of growth, the response of the photosynthetic apparatus to Cd and Pb differed significantly. While the application of Cd mostly caused a decrease in various photosynthetic parameters, the application of Pb stimulated some of them. Nevertheless, we discovered that the common features of the hormetic stimulation of shoot growth by heavy metals are an increase in the auxin and flavonol content and the maintenance of hydrogen peroxide at the same level as the control plants. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {40},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85085360825,

title = {Development and aging of photosynthetic apparatus of vitis vinifera L. During growing season},

author = { K. Sitko and S. Rusinowski and M. Pogrzeba and A. Daszkowska-Golec and Ż. Gieroń and H.M. Kalaji and E. Małkowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085360825&doi=10.32615%2fps.2019.107&partnerID=40&md5=915a57b5dae35616a9b4abf2b8edbb85},

doi = {10.32615/ps.2019.107},

issn = {03003604},

year  = {2020},

date = {2020-01-01},

journal = {Photosynthetica},

volume = {58},

number = {Special Issue},

pages = {186-193},

publisher = {Institute of Experimental Botany, ASCR},

abstract = {The aim of this study was to examine the development and aging of chosen grapevine leaves in situ during the growing season (130 d) using chlorophyll (Chl) a fluorescence measurements and determining the changes in pigment contents. During the course of photosystems development, the increase of Chl and decrease of anthocyanin contents in leaves was observed simultaneously. On 28th day, the maximum content of Chl and minimum content of anthocyanins was measured. However, the maximal photosynthetic performance was found one week later, when the content of Chl started to diminish. Our study proved that the achievement of maximal photosynthetic performance of each leaf took about quarter of organ life and this state lasted very shortly. In this work, we described and discussed for the first time the dynamics of Chl, anthocyanins, and flavonols combined with photosynthetic efficiency changes during the leaf life in situ. © The authors.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85076778671,

title = {Genetic and physiological dissection of photosynthesis in barley exposed to drought stress},

author = { A. Daszkowska-Golec and A. Collin and K. Sitko and A. Janiak and H.M. Kalaji and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076778671&doi=10.3390%2fijms20246341&partnerID=40&md5=14faebbe60c567c85947758707f74195},

doi = {10.3390/ijms20246341},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {24},

publisher = {MDPI AG},

abstract = {Balanced photosynthesis under drought is essential for better survival and for agricultural benefits in terms of biomass and yield. Given the current attempts to improve the photosynthetic efficiency for greater crop yield, the explanation of the genetic basis of that process, together with the phenotypic analysis, is significant in terms of both basic studies and potential agricultural application. Therefore, the main objective of this study was to uncover the molecular basis of the photosynthesis process under drought stress in barley. To address that goal, we conducted transcriptomic examination together with detailed photosynthesis analysis using the JIP-test. Using this approach, we indicated that photosynthesis is a process that is very early affected in barley seedlings treated with severe drought stress. Rather than focusing on individual genes, our strategy was pointed to the identification of groups of genes with similar expression patterns. As such, we identified and annotated almost 150 barley genes as crucial core-components of photosystems, electron transport components, and Calvin cycle enzymes. Moreover, we designated 17 possible regulatory interactions between photosynthesis-related genes and transcription factors in barley. Summarizing, our results provide a list of candidate genes for future genetic research and improvement of barley drought tolerance by targeting photosynthesis. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85072909603,

title = {Influence of short-term macronutrient deprivation in maize on photosynthetic characteristics, transpiration and pigment content},

author = { K. Sitko and Ż. Gieroń and M. Szopiński and P. Zieleźnik-Rusinowska and S. Rusinowski and M. Pogrzeba and A. Daszkowska-Golec and H.M. Kalaji and E. Małkowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072909603&doi=10.1038%2fs41598-019-50579-1&partnerID=40&md5=19c9ddd1e85d50ea693ffe9056d05506},

doi = {10.1038/s41598-019-50579-1},

issn = {20452322},

year  = {2019},

date = {2019-01-01},

journal = {Scientific Reports},

volume = {9},

number = {1},

publisher = {Nature Publishing Group},

abstract = {The aim of the research was to compare the impact of short-term deprivation of selected macronutrients (Ca; K; Mg and P) on the photosynthetic characteristics, transpiration and pigment content in maize. The strongest inhibition of photosynthesis was caused by a deprivation of Mg, which was visible as a decrease in the photosynthetic and transpiration rates, stomatal conductance, photosystem II (PSII) performance, chlorophyll and flavonol content with a simultaneously increased content of anthocyanins. In the K-deprived plants, a decrease in the photosynthetic rate was observed. However, the transpiration rate and stomatal conductance did not differ significantly compared with the control. In the K-deprived plants, a decrease in chlorophyll and an increase in the anthocyanin content were also observed. We showed that Ca starvation resulted in a decrease in the photosynthetic and transpiration rates, stomatal conductance and PSII performance, while the pigment content was not significantly different compared with the control. In the case of P-deprived plants, we observed a decrease in the photosynthetic and transpiration rates. Interestingly, the inhibition of stomatal conductance was the strongest in the P-deprived plants compared with all of the investigated elements. However, the performance of PSII was not significantly affected by P starvation compared with the control. Our results present for the first time a comprehensive analysis of the effect of short-term macronutrient deprivation on photosynthesis and transpiration in maize plants. © 2019, The Author(s).},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85067891720,

title = {New Miscanthus hybrids cultivated at a Polish metal-contaminated site demonstrate high stomatal regulation and reduced shoot Pb and Cd concentrations},

author = { S. Rusinowski and J. Krzyżak and J. Clifton-Brown and E. Jensen and M. Mos and R. Webster and K. Sitko and M. Pogrzeba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067891720&doi=10.1016%2fj.envpol.2019.06.062&partnerID=40&md5=d4c895f7e8fb1e2cb85c45ab6b317284},

doi = {10.1016/j.envpol.2019.06.062},

issn = {02697491},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Pollution},

volume = {252},

pages = {1377-1387},

publisher = {Elsevier Ltd},

abstract = {The increased bioeconomy targets for the biomass share of renewable energy production across Europe should be met using land unsuitable for food production. Miscanthus breeding programs targeted the production of plants with a diverse range of traits allowing a wider utilization of land resources for biofuel production without competing with arable crops. These traits include increasing tolerances to drought, chilling, and to metal(loid)s excess. Two novel Miscanthus hybrids, GNT41 and GNT34, were compared against Miscanthus x giganteus (Mxg) on metal-contaminated arable land in Poland. This study aimed at evaluating their yield, biomass quality and quantifying seasonal differences in photosynthetic and transpiration parameters. A secondary objective was to identify key physiological mechanisms underlying differences in metal accumulation between the investigated plants. The new hybrids produced a similar yield to Mxg (13–15 t ha−1 yr−1), had shorter shoots, higher Leaf Area Index and stem number. Based on gas exchange measurements, GNT34 exhibited isohydric (water-conserving) behavior. The stomatal response to light of the new hybrids was at least twice as fast as that of Mxg, a trait that is often associated with increased seasonal water use efficiency. This contributed to the almost 40% reduction in shoot Pb and Cd concentrations for the new hybrids as compared to Mxg. This suggested that promoting stomatal regulation in conjunction with improved water conservation may be a target for improving plants for wider use on metals contaminated land. © 2019 The Authors},

note = {21},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85064486941,

title = {Cultivation of C4 perennial energy grasses on heavy metal contaminated arable land: Impact on soil, biomass, and photosynthetic traits},

author = { S. Rusinowski and J. Krzyżak and K. Sitko and H.M. Kalaji and E. Jensen and M. Pogrzeba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064486941&doi=10.1016%2fj.envpol.2019.04.048&partnerID=40&md5=256118b694fefc133c2ec50c88802e67},

doi = {10.1016/j.envpol.2019.04.048},

issn = {02697491},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Pollution},

volume = {250},

pages = {300-311},

publisher = {Elsevier Ltd},

abstract = {The objective of this study was to evaluate the potential of three C4 perennial grasses (Miscanthus x giganteus; Panicum virgatum and Spartina pectinata) for biomass production on arable land unsuitable for food crop cultivation due to Pb, Cd and Zn contamination. We assessed soil properties, biomass yield, metal concentrations, and the photosynthetic performance of each species. Physico-chemical and elemental analyses were performed on soil samples before plantation establishment (2014) and after three years of cultivation (2016), when leaf area index, plant height, yield and heavy metal content of biomass were also determined. Physiological measurements (gas exchange; pigment content; chlorophyll a fluorescence) were recorded monthly between June and September on mature plants in 2016. Cultivation of investigated plants resulted in increased pH, nitrogen, and organic matter (OM) content in soil, although OM increase (13%) was significant only for S. pectinata plots. During the most productive months, maximal quantum yield values of primary photochemistry (Fv/Fm) and gas exchange parameter values reflected literature data of those plants grown on uncontaminated sites. Biomass yields of M. x giganteus (15.0 ± 0.4 t d.m. ha−1) and S. pectinata (12.6 ± 1.2 t d.m. ha−1) were also equivalent to data published from uncontaminated land. P. virgatum performed poorly (4.1 ± 0.4 t d.m. ha−1), probably due to unfavourable climatic conditions, although metal uptake in this species was the highest (3.6 times that of M. x giganteus for Pb). Yield and physiological measurements indicated that M. x giganteus and S. pectinata were unaffected by the levels of contamination and therefore offer alternatives for areas where food production is prohibited. The broad cultivatable latitudinal range of these species suggests these results are widely relevant for development of the bioeconomy. We recommend multi-location trials under diverse contaminant and environmental regimes to determine the full potential of these species. © 2019 Elsevier LtdMiscanthus and cordgrass offer food production alternatives due to high yield potential on heavy metal contaminated arable land. © 2019 Elsevier Ltd},

note = {23},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85068432292,

title = {Toxic effects of cd and zn on the photosynthetic apparatus of the Arabidopsis halleri and Arabidopsis arenosa pseudo-metallophytes},

author = { M. Szopiński and K. Sitko and Ż. Gieroń and S. Rusinowski and M. Corso and C. Hermans and N. Verbruggen and E. Małkowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068432292&doi=10.3389%2ffpls.2019.00748&partnerID=40&md5=4431c7b5000ee92ff389fa16385f38cf},

doi = {10.3389/fpls.2019.00748},

issn = {1664462X},

year  = {2019},

date = {2019-01-01},

journal = {Frontiers in Plant Science},

volume = {10},

publisher = {Frontiers Media S.A.},

abstract = {Hyperaccumulation and hypertolerance of Trace Metal Elements (TME) like Cd and Zn are highly variable in pseudo-metallophytes species. In this study we compared the impact of high Cd or Zn concentration on the photosynthetic apparatus of the Arabidopsis arenosa and Arabidopsis halleri pseudo-metallophytes growing on the same contaminated site in Piekary Slaskie in southern Poland. Plants were grown in hydroponic culture for 6 weeks, and then treated with 1.0 mM Cd or 5.0 mM Zn for 5 days. Chlorophyll a fluorescence and pigment content were measured after 0, 1, 2, 3, 4, and 5 days in plants grown in control and exposed to Cd or Zn treatments. Moreover, the effect of TME excess on the level of oxidative stress and gas-exchange parameters were investigated. In both plant species, exposure to high Cd or Zn induced a decrease in chlorophyll and an increase in anthocyanin contents in leaves compared to the control condition. After 5 days Cd treatment, energy absorbance, trapped energy flux and the percentage of active reaction centers decreased in both species. However, the dissipated energy flux in the leaves of A. arenosa was smaller than in A. halleri. Zn treatment had more toxic effect than Cd on electron transport in A. halleri compared with A. arenosa. A. arenosa plants treated with Zn excess did not react as strongly as in the Cd treatment and a decrease only in electron transport flux and percentage of active reaction centers compared with control was observed. The two species showed contrasting Cd and Zn accumulation. Cd concentration was almost 3-fold higher in A. arenosa leaves than in A. halleri. On the opposite, A. halleri leaves contained 3-fold higher Zn concentration than A. arenosa. In short, our results showed that the two Arabidopsis metallicolous populations are resistant to high Cd or Zn concentration, however, the photosynthetic apparatus responded differently to the toxic effects. © 2019 Szopiński, Sitko, Gieroń, Rusinowski, Corso, Hermans, Verbruggen and Małkowski.},

note = {46},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85058948957,

title = {How autochthonous microorganisms influence physiological status of Zea mays L. cultivated on heavy metal contaminated soils?},

author = { S. Rusinowski and A. Szada-Borzyszkowska and P. Zieleźnik-Rusinowska and E. Małkowski and J. Krzyżak and G. Woźniak and K. Sitko and M. Szopiński and J.P. McCalmont and H.M. Kalaji and M. Pogrzeba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058948957&doi=10.1007%2fs11356-018-3923-9&partnerID=40&md5=ed246b6eba8d11082baf8d65cc55c8ed},

doi = {10.1007/s11356-018-3923-9},

issn = {09441344},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {5},

pages = {4746-4763},

publisher = {Springer Verlag},

abstract = {The aim of this study was to investigate the effect of autochthonous microorganisms present in soil collected from heavy metal (HM) uncontaminated (Pb ≈ 59 mg kg −1 ; Cd ≈ 0.4 mg kg −1 ; Zn ≈ 191 mg kg −1 ), moderately (Pb ≈ 343 mg kg −1 ; Cd ≈ 12 mg kg −1 ; Zn ≈ 1876 mg kg −1 ), and highly (Pb ≈ 1586 mg kg −1 ; Cd ≈ 57 mg kg −1 ; Zn ≈ 3280 mg kg −1 ) contaminated sites on Zea mays elemental composition, physiological status, and growth parameters. For this purpose, half of the collected soil was sterilized and soil characterization was performed. After 45 days of cultivation, the presence of HM in the soil negatively affected photosynthesis and transpiration rates, relative chlorophyll content, anthocyanins index, chlorophyll fluorescence parameters, and content of oxidative stress products (H 2 O 2 and Malondialdehyde) of Zea mays, while soil sterilization had a positive effect on those parameters. Average percentage of colonization of root segments by arbuscular mycorrhiza fungi decreased with an increase of HM contamination in the soil. The increase in shoot concentration of HMs, particularly Cd and Zn, was a result of contaminated soils sterilization. Aboveground biomass of maize cultivated on sterilized soil was 3-fold, 1.5-fold, and 1.5-fold higher for uncontaminated, moderately contaminated and highly contaminated soils respectively when compared to nonsterilized soils. Contrary to our expectation, autochthonous microflora did not improve plant growth and photosynthetic performance; in fact, they had a negative effect on those processes although they did reduce concentration of HMs in the shoots grown on contaminated soils. © 2018, The Author(s).},

note = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85040325592,

title = {Prompt chlorophyll fluorescence as a tool for crop phenotyping: an example of barley landraces exposed to various abiotic stress factors},

author = { H.M. Kalaji and A. Rastogi and M. Živčák and M. Brestic and A. Daszkowska-Golec and K. Sitko and K.Y. Alsharafa and R. Lotfi and P. Stypiński and I.A. Samborska and M.D. Cetner},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040325592&doi=10.1007%2fs11099-018-0766-z&partnerID=40&md5=82ab27144de33dedba832444496b1909},

doi = {10.1007/s11099-018-0766-z},

issn = {03003604},

year  = {2018},

date = {2018-01-01},

journal = {Photosynthetica},

volume = {56},

number = {3},

pages = {953-961},

publisher = {The Institute of Experimental Biology of the Czech Academy of Sciences},

abstract = {The study examined photosynthetic efficiency of two barley landraces (cvs. Arabi Abiad and Arabi Aswad) through a prompt fluorescence technique under influence of 14 different abiotic stress factors. The difference in the behavior of photosynthetic parameters under the same stress factor in–between cv. Arabi Abiad and cv. Arabi Aswad indicated different mechanisms of tolerance and strategies for the conversion of light energy into chemical energy for both the landraces. This study confirmed the suitability of some chlorophyll fluorescence parameters as reliable biomarkers for screening the plants at the level of photosynthetic apparatus. © 2018, The Author(s).},

note = {122},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85032903556,

title = {Can chlorophyll-a fluorescence parameters be used as bio-indicators to distinguish between drought and salinity stress in Tilia cordata Mill?},

author = { H.M. Kalaji and L. Račková and V. Paganová and T. Swoczyna and S. Rusinowski and K. Sitko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032903556&doi=10.1016%2fj.envexpbot.2017.11.001&partnerID=40&md5=28437aaeead5bc5ea818da3342cb4ccb},

doi = {10.1016/j.envexpbot.2017.11.001},

issn = {00988472},

year  = {2018},

date = {2018-01-01},

journal = {Environmental and Experimental Botany},

volume = {152},

pages = {149-157},

publisher = {Elsevier B.V.},

abstract = {Chlorophyll-a fluorescence measurements have been used (for many years) to study the function and performance of photosynthetic machinery of various plants. However, only few recent works have shown that this tool can be useful to identify stress factor that affects or/and limits plant growth. The aim of our research was to identify chlorophyll fluorescence parameters which can be used as indicators to drought or/and salinity stress in Tilia cordata Mill. Young potted trees (1–1.5 m high with a trunk of 2–3 cm in diameter) were exposed to drought stress or salinity stress for 4 weeks under semi-controlled conditions in a greenhouse. Chlorophyll-a fluorescence measurements were conducted every week four times during the stress treatment. The fluorescence parameters based on OJIP transient curves were calculated in order to ascertain which of them was affected by drought and/or salinity. We found that salinity and drought had similar mechanisms of action on the light-depend photosynthesis phase, however the response of photosystem II to applied stress occurred earlier in drought-stressed plants. Changes appeared as damage to the oxygen-evolving complex and reaction centres with a simultaneous increase in dissipated energy. Since both stress factors induced similar photo-inhibitory influence on the light-depending photosynthesis phase, the chlorophyll-a fluorescence tool cannot be recommended as a bio-indicator to distinguish between drought and salinity stress effects in Tilia cordata Mill. © 2017 Elsevier B.V.},

note = {101},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85040322145,

title = {Mutation in barley ERA1 (Enhanced Response to ABA1) gene confers better photosynthesis efficiency in response to drought as revealed by transcriptomic and physiological analysis},

author = { A. Daszkowska-Golec and A. Collin and K. Sitko and M. Słota and M.M. Kurowska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040322145&doi=10.1016%2fj.envexpbot.2018.01.003&partnerID=40&md5=0007d532bbcbed5056c705585b2fd044},

doi = {10.1016/j.envexpbot.2018.01.003},

issn = {00988472},

year  = {2018},

date = {2018-01-01},

journal = {Environmental and Experimental Botany},

volume = {148},

pages = {12-26},

publisher = {Elsevier B.V.},

abstract = {Farnesylation is a post-translational modification that promotes the interaction between the modified signaling protein and membrane lipids and/or other proteins. Farnesyltransferase is the crucial enzyme involved in this process. Strikingly, plant mutants in the ERA1 (Enhanced response to ABA 1) gene, encoding β-subunit of farnesyltransferase, exhibited ABA-hypersensitivity during seed germination and drought tolerance in several species including Arabidopsis, wheat and soybean. However, the mechanism of ERA1 action has not been resolved yet. Here, we present the potential regulatory role of ERA1 in the drought signaling network in barley. With the aim of decoding the role of the ERA1 gene, we developed a unique barley mutant using TILLING analysis. Mutation in HvERA1 confers semi-dwarf phenotype, ABA-sensitivity during seed germination and drought tolerance. Our transcriptomic analysis suggested a role of HvERA1 in regulation of the crosstalk between ABA and ethylene at the onset of drought. Furthermore, analysis of hvera1.b response to prolonged drought stress linked HvERA1 to the metabolism of galactolipids, that build the chloroplast membranes. It might results in the protection of hvera1.b photosystem and thus, in its better photosynthesis performance under water stress. Together, these results indicate the possible mechanism of the primary cause of the observed alterations in the hvera1.b mutant. © 2018 Elsevier B.V.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85029223648,

title = {Photosynthetic efficiency as bioindicator of environmental pressure in A. halleri},

author = { K. Sitko and S. Rusinowski and H.M. Kalaji and M. Szopiński and E. Małkowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029223648&doi=10.1104%2fpp.17.00212&partnerID=40&md5=9f9fc0b28706273baccb1cea45b86305},

doi = {10.1104/pp.17.00212},

issn = {00320889},

year  = {2017},

date = {2017-01-01},

journal = {Plant Physiology},

volume = {175},

number = {1},

pages = {290-302},

publisher = {American Society of Plant Biologists},

abstract = {In earlier ecophysiological studies that were conducted on Arabidopsis halleri plants, scientists focused on the mechanisms of Cd and Zn hyperaccumulation but did not take into consideration the environmental factors that can significantly affect the physiological responses of plants in situ. In this study, we investigated A. halleri that was growing on two nonmetalliferous and three metalliferous sites, which were characterized by different environmental conditions. We compared these populations in order to find differences within the metallicolous and nonmetallicolous groups that have not yet been investigated. The concentrations of several elements in the plant and soil samples also were investigated. To our knowledge, the concentration and fluorescence of chlorophyll were measured for A. halleri in situ for the first time. Our study confirmed the hyperaccumulation of Cd and Zn for each metallicolous population. For the metallicolous populations, the inhibition of parameters that describe the efficiency of the photosynthetic apparatus with increasing accumulations of heavy metals in the shoots also was observed. It was found that the nonmetallicolous plant populations from the summit of Ciemniak Mountain had larger antenna dimensions and chlorophyll content but a lower percentage of active reaction centers. To our knowledge, in this study, the internal high physiological diversity within the populations that inhabit metalliferous and nonmetalliferous sites is presented for the first time. © 2017 American Society of Plant Biologists. All rights reserved.},

note = {43},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021152716,

title = {Mutation in HvCBP20 (Cap binding protein 20) adapts barley to drought stress at phenotypic and transcriptomic levels},

author = { A. Daszkowska-Golec and A. Collin and M. Marzec and M. Słota and M.M. Kurowska and M. Gajecka and P. Gajewska and T. Płociniczak and K. Sitko and A. Pacak and Z. Szweykowska-Kulinska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021152716&doi=10.3389%2ffpls.2017.00942&partnerID=40&md5=769fb24d1f52b6fc895f399773d7ea69},

doi = {10.3389/fpls.2017.00942},

issn = {1664462X},

year  = {2017},

date = {2017-01-01},

journal = {Frontiers in Plant Science},

volume = {8},

publisher = {Frontiers Media S.A.},

abstract = {CBP20 (Cap-Binding Protein 20) encodes a small subunit of the cap-binding complex (CBC), which is involved in the conserved cell processes related to RNA metabolism in plants and, simultaneously, engaged in the signaling network of drought response, which is dependent on ABA. Here, we report the enhanced tolerance to drought stress of barley mutant in the HvCBP20 gene manifested at the morphological, physiological, and transcriptomic levels. Physiological analyses revealed differences between the hvcbp20.ab mutant and its WT in response to a water deficiency. The mutant exhibited a higher relative water content (RWC), a lower stomatal conductance and changed epidermal pattern compared to the WT after drought stress. Transcriptome analysis using the Agilent Barley Microarray integrated with observed phenotypic traits allowed to conclude that the hvcbp20.ab mutant exhibited better fitness to stress conditions by its much more efficient and earlier activation of stress-preventing mechanisms. The network hubs involved in the adjustment of hvcbp20.ab mutant to the drought conditions were proposed. These results enabled to make a significant progress in understanding the role of CBP20 in the drought stress response. © 2017 Daszkowska-Golec, Skubacz, Marzec, Slota, Kurowska, Gajecka, Gajewska, Płociniczak, Sitko, Pacak, Szweykowska-Kulinska and Szarejko.},

note = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85016433200,

title = {Relationships between soil parameters and physiological status of Miscanthus x giganteus cultivated on soil contaminated with trace elements under NPK fertilisation vs. microbial inoculation},

author = { M. Pogrzeba and S. Rusinowski and K. Sitko and J. Krzyżak and A. Skalska and E. Małkowski and D. Ciszek and S. Werle and J.P. McCalmont and M. Mos and H.M. Kalaji},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016433200&doi=10.1016%2fj.envpol.2017.03.058&partnerID=40&md5=dada2118b0044028b6e2d515be9a120c},

doi = {10.1016/j.envpol.2017.03.058},

issn = {02697491},

year  = {2017},

date = {2017-01-01},

journal = {Environmental Pollution},

volume = {225},

pages = {163-174},

publisher = {Elsevier Ltd},

abstract = {Crop growth and development can be influenced by a range of parameters, soil health, cultivation and nutrient status all play a major role. Nutrient status of plants can be enhanced both through chemical fertiliser additions (e.g. N; P; K supplementation) or microbial fixation and mobilisation of naturally occurring nutrients. With current EU priorities discouraging the production of biomass on high quality soils there is a need to investigate the potential of more marginal soils to produce these feedstocks and the impacts of soil amendments on crop yields within them. This study investigated the potential for Miscanthus x giganteus to be grown in trace element (TE)-contaminated soils, ideally offering a mechanism to (phyto)manage these contaminated lands. Comprehensive surveys are needed to understand plant-soil interactions under these conditions. Here we studied the impacts of two fertiliser treatments on soil physico-chemical properties under Miscanthus x giganteus cultivated on Pb, Cd and Zn contaminated arable land. Results covered a range of parameters, including soil rhizosphere activity, arbuscular mycorrhization (AM), as well as plant physiological parameters associated with photosynthesis, TE leaf concentrations and growth performance. Fertilization increased growth and gas exchange capacity, enhanced rhizosphere microbial activity and increased Zn, Mg and N leaf concentration. Fertilization reduced root colonisation by AMF and caused higher chlorophyll concentration in plant leaves. Microbial inoculation seems to be a promising alternative for chemical fertilizers, especially due to an insignificant influence on the mobility of toxic trace elements (particularly Cd and Zn). © 2017 Elsevier Ltd},

note = {44},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84923397435,

title = {The effect of abscicsic acid and dimethyl sulfoxide and different temperatures on the cryopreservation process of Abies nordmanniana embryogenic callus},

author = { K. Nawrot-Chorabik and K. Sitko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923397435&doi=10.12905%2f0380.phyton54%282%292014-0275&partnerID=40&md5=89cff78c161e319f93ddfa9747130ac7},

doi = {10.12905/0380.phyton54(2)2014-0275},

issn = {00792047},

year  = {2014},

date = {2014-01-01},

journal = {Phyton - Annales Rei Botanicae},

volume = {54},

number = {2},

pages = {275-282},

publisher = {Verlag Ferdinand Berger und Sohne GmbH},

abstract = {The research on the Nordmann fir's callus cryopreservation showed that comparing the two tested cryoprotectants, the pretreatment-phase-introduced abscisic acid (ABA), the protectant that plays an important role in the water management regulation and adaptation to extreme environments (including low temperatures) proved to be more effective than dimethyl sulfoxide (DMSO), which is applied on a larger scale, but mutagenic cryoprotectant. Moreover, the use of sudden temperature changes during the process of rapid freezing and thawing caused a 65% increase in the survival rate in comparison to slow freezing and thawing of A. nordmanniana callus tissues.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}