@article{2-s2.0-85171778833,

title = {Assessing the Risk of Groundwater Pollution in Northern Algeria through the Evaluation of Influencing Parameters and Ensemble Methods},

author = { S. Tachi and H. Bouguerra and M. Djellal and O. Benaroussi and A. Belaroui and B. Łozowski and M. Augustyniak and S. Benmamar and S. Benziada and A. Woźnica},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85171778833&doi=10.1134%2fS1028334X23600767&partnerID=40&md5=5fba9f721fd68500590b1bb141ebd127},

doi = {10.1134/S1028334X23600767},

issn = {1028334X},

year  = {2023},

date = {2023-01-01},

journal = {Doklady Earth Sciences},

volume = {513},

number = {1},

pages = {1233-1243},

publisher = {Pleiades Publishing},

abstract = {Abstract: Developing an accurate map to control groundwater pollution is becoming increasingly important to fulfill requirements of the Algerian water directives. Evaluating groundwater vulnerability to nitrate contamination in the eastern Mitidja aquifer has become crucial for water resource control and preservation. In this research, some of the commonly used ensemble methods namely Categorical Boosting, Adaptive Boosting and Random Forest were employed to model the spatial groundwater vulnerability to nitrate concentration based on the maximum acceptable concentration in drinking water according to the Algerian directives (50 mg/L). The study were carried out on the evaluation of different possible influencing parameters including depth to groundwater, permeability, rainfall, vadose zone, distance to river, drainage density, land use land cover, Normalized Difference Vegetation Index, slope, topographic wetness index and elevation. The proposed approach aims to understand and control the vulnerability and pollution process of the groundwater aquifers with the smallest available dataset to assess vulnerability predictive mapping. The best results based on the receiver operating characteristic and area under the curve (ROC-AUC) showed that the Categorical boosting indicated the highest accuracy of 94% then the adaptive boosting algorithm with 92%, and the Random forest algorithm with 89%. It revealed that climatic and hydrogeological factors are the key factors determining groundwater vulnerability to NO3 concentration in the eastern Mitidja. It is concluded that the risk of groundwater pollution covers a large area of the groundwater resources in the city of Algiers, Blida, and Boumerdas in northern Algeria. © 2023, Pleiades Publishing, Ltd.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85149706025,

title = {Detection of changes in the hydrobiological parameters of the Oder River during the ecological disaster in July 2022 based on multi-parameter probe tests and remote sensing methods},

author = { D. Absalon and M. Matysik and A. Woźnica and N. Janczewska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149706025&doi=10.1016%2fj.ecolind.2023.110103&partnerID=40&md5=86f1cfc17a45108877a190a1034d1dfc},

doi = {10.1016/j.ecolind.2023.110103},

issn = {1470160X},

year  = {2023},

date = {2023-01-01},

journal = {Ecological Indicators},

volume = {148},

publisher = {Elsevier B.V.},

abstract = {Starting from the second half of July, hydrological conditions (low water levels and low flows) and atmospheric conditions (high temperatures; lack of precipitation) contributed to algae bloom in the Oder River basin. The mass extinction of fish in the middle and lower section of the Oder was probably the result of such environmental conditions. Because algae bloom is correlated with an increase in the content of chlorophyll in the river and on its surface, the authors focused on an analysis of changes in the chlorophyll amount in the longitudinal profile of the river. The key data was obtained by soundings carried out at an interval of about one kilometre during the research expedition, which took place on July 16–24, 2022. These data coincided with the values of the NDVI index (normalised difference vegetation index) based on 10-metre satellite images from the Sentinel 2 mission. Imagery from cloudless periods (mainly March; June; July and August) processed using the NDVI index was compared. Regularly elevated chlorophyll levels in the upstream section (occurring in each period) and temporarily elevated chlorophyll values as moving downstream were observed. This article confirms the initial conclusions of Kolada et al. (2022) that the mass fish kill in the Oder River in the summer of 2022 was conditioned by natural environmental pressure causing massive algae bloom and the release of their toxins. The article also proves that the use of satellite images for this type of analysis is the correct approach, but due to low resolution, they cannot be used as reference material. Changes in the hydrobiological parameters of the river, which are a direct indicator of the causes of the ecological disaster, are the data of the multi-parameter probe obtained two weeks before the first mass catches of dead fish. © 2023},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85149932914,

title = {Analysis of the Salinity of the Vistula River Based on Patrol Monitoring and State Environmental Monitoring},

author = { A. Woźnica and D. Absalon and M. Matysik and M. Bąk and A. Cieplok and D. Halabowski and A. Koczorowska and M. Krodkiewska and M. Libera and E.M. Sierka and A. Spyra and R. Czerniawski and Ł. Sługocki and B. Łozowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149932914&doi=10.3390%2fw15050838&partnerID=40&md5=a1b9ba5b610a3021fd3cad70380682bc},

doi = {10.3390/w15050838},

issn = {20734441},

year  = {2023},

date = {2023-01-01},

journal = {Water (Switzerland)},

volume = {15},

number = {5},

publisher = {MDPI},

abstract = {Background: Secondary salinity of river water reduces the value of ecosystem services, negatively impacting the entire aquatic ecosystem and reducing the possibility of water use. In Poland, significant anthropogenic salinity of rivers and water reservoirs is usually associated with mining activity consisting of pumping salty mine water into settling ponds or often directly into rivers. However, to assess the reasons for the salinity of the Vistula waters, it is necessary to identify all sources of salt in surface waters, enabling the assessment of the salt load in the waters. Methods: The paper presents four sources of data which have been compiled to propose a valuable method for analyzing the threat of the river. Patrol monitoring was one method of data acquisition, and State Environmental Monitoring data ware also used. Clustering and correlation statistical techniques were used for analysis. Results: Of the 20 physical and chemical parameters analyzed, chloride, calcium sulphate and magnesium ions are important for salinity. Measurements with multi-parameter probes allowed for the identification of increased salinity pressure sites, while flow analyses were required to calculate the load. Conclusions: The Vistula River had the highest concentrations of the analyzed ions in the Silesia Region. The use of patrol monitoring can be highly useful in determining the causes of emerging problems with water quality and supporting State Environmental Monitoring. © 2023 by the authors.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85147301610,

title = {Combined complementary imaging techniques in morphological analysis of Spongilla lacustris (Linnaeus 1759)},

author = { A. Woźnica and J. Karczewski and R. Gwiazda and J. Karcz and T. Bernaś and M. Libera and A. Babczyńska and B. Łozowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85147301610&doi=10.1002%2flom3.10534&partnerID=40&md5=11eb042255adfefe285c387664cfd151},

doi = {10.1002/lom3.10534},

issn = {15415856},

year  = {2023},

date = {2023-01-01},

journal = {Limnology and Oceanography: Methods},

volume = {21},

number = {3},

pages = {141-151},

publisher = {John Wiley and Sons Inc},

abstract = {Sponges, like Spongilla lacustris, as filter feeders, play an essential role in water purification in aquatic ecosystems. The body of this demosponge, in general, consists of both organic soft skeleton and a siliceous scaffold. Their construction of the spicule-bundling scaffold as mechanical support for skeletal organic mesohyl seems crucial for filtration efficiency. Understanding the structure of the sponge's biosilica-based scaffold as well as its location within three-dimensional (3D) skeletal construct requires the introduction of effective analytical methods. The investigations focused on the morphology and architecture of skeletal elements of S. lacustris utilizing the combination of X-ray computed microtomography (μCT) and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM/EDS). The construction details, surface morphology and chemical composition of the sponge scaffold are presented. μCT provided the reconstructed 3D images of skeleton structures, including longitudinally and transversely oriented bundles of overlapping spicules, forming a ladder-like construction as well as the length, geometric distribution, and the surface of the spicules. Further analyses based on SEM/EDS confirmed the proper identification of the structures and their localization and revealed a high abundance of silicon and a low amount of carbon and oxygen in spicules, high abundance of silicon, carbon and oxygen in layered membranes surrounding the bundles of spicules but predominating carbon in the pinacoderm. Combination of these techniques provides a unique image of the sponge body morphology. Verified set of tools may be used for further analyses of sponge body mechanics. © 2023 Association for the Sciences of Limnology and Oceanography.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85133266871,

title = {Modeling of Nitrification Kinetics in a Respirometric Biosensor under Suboptimal Conditions},

author = { A. Woźnica and J. Karczewski and C. Kliś and J. Długosz and P. Ziemski and A. Nowak and T. Bernaś},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85133266871&doi=10.3390%2fw14132031&partnerID=40&md5=1e4f5a74cdc187870d4cd664f6a7e521},

doi = {10.3390/w14132031},

issn = {20734441},

year  = {2022},

date = {2022-01-01},

journal = {Water (Switzerland)},

volume = {14},

number = {13},

publisher = {MDPI},

abstract = {Sensitive detection with cell biosensors requires optimization of their working conditions and standardization of the response in variable physicochemical conditions. The introduction of an analyte to a sensor, which contributes to this variability, may account for the modeling of microbial metabolism. We constructed a multiparameter model of a water toxicity sensor of Automatic Biodetector for Water Toxicity (ABTOW), developed by our group and based on nitrifying bacteria. The model describes the kinetics of nitrification as a function of four orthogonal parameters: temperature, pH, oxygen and ammonium concentration. Furthermore, we characterized the signal-to-noise ratio (SNR) of the ABTOW readout as a function of these parameters. Thus, a region of parameter space corresponding to optimal ABTOW operation is identified and its sensitivity quantified. We applied the model to describe the ABTOW performance in non-equilibrium conditions produced by rapid changes in pH and temperature. In sum, the model based on four physicochemical parameters describes changes in the biosensor's activity, the biological element of which are nitrifying bacteria characterized by simple chemolithoautotrophic metabolism. The description of reaction kinetics through multiparameter modeling in combination with stability analysis can find application in process control in biotechnology, biodetection and environmental research. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85135441745,

title = {The spatial structure (3D) and mechanical properties of the sponge Spongilla lacustris L. (Porifera: Spongillida) skeleton as a potential tensegral architecture},

author = { A. Woźnica and J. Karczewski and T. Bernaś and P. Świątek and M. Drab and R. Surma and M. Krzyżowski and B. Łozowski and R. Gwiazda and M. Libera and D. Absalon and A. Babczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135441745&doi=10.1080%2f24750263.2022.2105964&partnerID=40&md5=d32c8660b692cf631fb930f88c6b6495},

doi = {10.1080/24750263.2022.2105964},

issn = {24750263},

year  = {2022},

date = {2022-01-01},

journal = {European Zoological Journal},

volume = {89},

number = {1},

pages = {1002-1017},

publisher = {Taylor and Francis Ltd.},

abstract = {Complex biological systems often provide ready solutions for contemporary engineering. One such organism might be sponges, primitive, tissueless animals whose evolution over 600 million years has allowed them to become highly specialized. An example of such an organism is the freshwater sponge Spongilla lacustris L., an organism that filters water. This study aimed to investigate the 3D structure of the aforementioned sponge using a broad spectrum of techniques such as Microcomputed Tomography (µCT), Scanning Electron Microscopy (SEM), Confocal Laser Scanning Microscopy (CLSM), and Light Microscopy. Additionally, these techniques have been used to correlate sponge architecture with mechanical properties using the concept of tensegrity, i.e., the feature of architectural structures that self-stabilize by balancing multidirectional, often opposing, tensile and compressive forces. A more detailed look at the structure of the sponge skeleton reveals that it is based on two elements: rigid siliceous spicules, chitin in in fibres with cementing collagen-type spongin material. The coexistence of these elements in the sponge structure determines the mechanical properties and, consequently, the sponge skeleton’s postulated tensegrity. Our observations indicate that the integrity of loose megascleres is realized by sponging material surrounding the bundles of spicules. Our distinction of skeletal elements was determined by the number of spicules in the bundle, the direction of spicule position relative to the main body axis, and the way the elements were connected. The arrangement of the bundles described above has important implications for the mechanical properties of the sponge skeleton and, consequently, for the tensegrity hypothesis. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85128691739,

title = {Microbiome dynamics modeling and analysis in relation to spatio-temporal changes in physicochemical conditions of the water ecosystem},

author = { A. Woźnica and M. Kwaśniewski and K. Chwiałkowska and B. Łozowski and D. Absalon and M. Libera and M. Krzyżowski and A. Babczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128691739&doi=10.24425%2faep.2022.140542&partnerID=40&md5=62aef1f191ab748b40b4223bc7d4161e},

doi = {10.24425/aep.2022.140542},

issn = {20834772},

year  = {2022},

date = {2022-01-01},

journal = {Archives of Environmental Protection},

volume = {48},

number = {1},

pages = {18-30},

publisher = {Polska Akademia Nauk},

abstract = {In this paper, we consider the development of reliable tools to assess the water quality and state of aquatic ecosystems in dynamic conditions a crucial need to address. One of such tools could be devised by monitoring the taxonomic structure of reservoirs’ microbiomes. Microbial taxa’s ecological and metabolic characteristics suggest their essential roles in maintaining the water ecosystem’s environmental equilibrium. The study aimed to explain the role of diversity and seasonal variability of the microbial communities in the ecosystem stability on the example of Goczałkowice Reservoir (Poland). The structure of the reservoir microbiome was studied using bioinformatics and modeling techniques. Water was sampled periodically in July & November 2010, and April 2011 at four representative sites. The abundance and relative fraction of the limnetic taxonomic units were determined in respect to the physicochemical indices. Significant seasonal variations in the number of operational taxonomic units (OTU) were observed within the reservoir basin’s main body but not at the main tributary’s mouth. The highest values of the correlation coefficients between OTU and physicochemical variables were obtained for Burkholderiales, Pseudoanabenales, Rickettsiales, Roseiflexales, Methylophilales, Actinomycetales, and Cryptophyta. These microorganisms are proposed as indicators of environmental conditions and water quality. Metataxonomic analyses of the freshwater microbiome in the reservoir, showed that microorganisms constitute conservative communities that undergo seasonal and local changes regarding the relative participation of the identified taxa. Therefore, we propose that monitoring those variations could provide a reliable measure of the state of aquatic ecosystems. © 2022. The Author(s).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85092466603,

title = {Phytoplankton production in relation to simulated hydro- and thermodynamics during a hydrological wet year – Goczałkowice reservoir (Poland) case study},

author = { R. Ulańczyk and C. Kliś and B. Łozowski and A. Babczyńska and A. Woźnica and J. Długosz and E. Wilk-Woźniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092466603&doi=10.1016%2fj.ecolind.2020.106991&partnerID=40&md5=083d95fedb5bef14691dbc936291242c},

doi = {10.1016/j.ecolind.2020.106991},

issn = {1470160X},

year  = {2021},

date = {2021-01-01},

journal = {Ecological Indicators},

volume = {121},

publisher = {Elsevier B.V.},

abstract = {Phytoplankton is one of the crucial components of water body ecosystems. Its presence and development depend on biological, physical and chemical factors and in consequence it is an important indicator of ecosystem condition. Monitoring of phytoplankton production, measured as chlorophyll a concentration, is a useful tool for assessing the status of dam reservoirs. Modeled chlorophyll a concentrations are used as water quality indicators in locations not included in monitoring systems, in situations when the temporal resolution of the monitoring is not enough, and in assessments of the impacts of future activities. Therefore, the aim of this study was to find correlations between hydro- and thermodynamics and the chlorophyll a concentration for possible application in reservoir monitoring and management, using an ELCOM-CAEDYM model. The analysis included summer and fall which are most prone to algal blooms, and four phytoplankton groups identified as dominant in the reservoir based on periodic observations. Comparisons of simulated water temperature and both observed and simulated chlorophyll a concentrations confirmed that these variables are significantly correlated (correlation of hourly chlorophyll a and water temperature was 0.70; ranging from 0.55 to 0.81 in the bottom and surface water layers; respectively; while for daily outputs it was 0.74; ranging from 0.60 to 0.83). This relation was stronger than that of chlorophyll a to nutrient (N; P and Si) concentrations. What is more, the method used allowed the assessment of a much more detailed spatial and temporal distribution of phytoplankton groups compared with conventional monitoring techniques. The study indicated that the phytoplankton community was dominated by Chlorophytes and Diatoms with a larger share of Chlorophytes in shallow parts of the reservoir. This domination was weaker after short water mixing events in summer and especially after the fall turnover. The increase in phytoplankton diversity was estimated to occur mainly near the surface and in shallow parts of the reservoir. Most of the observed concentrations of individual phytoplankton groups differed from simulation results by less than 25% and the model reflected accurately 74% of observed trends in concentrations. Calculated chlorophyll a concentration was well matched to hourly monitoring data (mean squared error = 5.6; Nash–Sutcliffe model efficiency coefficient = 0.51; Pearson correlation coefficient = 0.72 and p-value = 0.0007). High compatibility of the model to the values measured in the reservoir make it a promising tool for the prediction and planning of actions aimed at maintaining good functioning of the reservoir. © 2020 The Authors},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85102487461,

title = {Stress proteins concentration in caged Cyprinus carpio as a tool to monitor ecological stability in a model dam reservoir},

author = { A. Babczyńska and M. Tarnawska and P. Łaszczyca and P. Migula and B. Łozowski and A. Woźnica and I. Irnazarow and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102487461&doi=10.24425%2faep.2021.136452&partnerID=40&md5=5aafdbafbb462de53a29be6faf4a67ef},

doi = {10.24425/aep.2021.136452},

issn = {20834772},

year  = {2021},

date = {2021-01-01},

journal = {Archives of Environmental Protection},

volume = {47},

number = {1},

pages = {101-111},

publisher = {Polska Akademia Nauk},

abstract = {Variability of stress proteins concentration in caged carp exposed to transplantation experiment model dam reservoir was caused only by natural (climatic and biological) conditions. Thus, the reference data of stress proteins concentration range in young carp individuals were obtained. Metallothionein, HSP70 and HSP90 protein concentrations as biomarkers were assayed in the livers, gills and muscles of six-month-old (summer) or nine-month-old (autumn) carp individuals in relation to the site of encaging, season (summer or autumn), the term of sampling (1; 2 or 3 weeks after the transplantation) and tissue. Physicochemical analyses of the condition of water as well as pollution detection were conducted during each stage of the experiment. As the result of this study, the range of the variability of the stress protein concentration in young carp individuals was obtained. According to the analyses of the aquatic conditions of a reservoir with no detectable pollutants, we conclude that the variability in the stress protein concentration levels in the groups that were compared is solely the result of the natural conditions. Future regular monitoring of the reservoir using the transplantation method and young carp individuals will be both possible and reliable. Moreover, the range of variability in the stress protein concentrations that were measured in the young C. carpio individuals acquired from the model dam reservoir in relation to all of the studied factors may be applied in the monitoring of any other similar reservoir. © 2021. The Author(s).},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85084176121,

title = {Multi-faceted environmental analysis to improve the quality of anthropogenic water reservoirs (Paprocany reservoir case study)},

author = { D. Absalon and M. Matysik and A. Woźnica and B. Łozowski and W. Jarosz and R. Ulańczyk and A. Babczyńska and A. Pasierbiński},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084176121&doi=10.3390%2fs20092626&partnerID=40&md5=cc466385954de533dfd5f2601a334e34},

doi = {10.3390/s20092626},

issn = {14248220},

year  = {2020},

date = {2020-01-01},

journal = {Sensors (Switzerland)},

volume = {20},

number = {9},

publisher = {MDPI AG},

abstract = {Maintaining good condition of dam reservoirs in urban areas seems increasingly important due to their valuable role in mitigating the effects of global warming. The aim of this study is to analyze possibilities to improve water quality and ecosystem condition of the Paprocany dam reservoir (highly urbanized area of southern Poland) using current data of the water parameters, historical sources, and DPSIR (Driver–Pressure–State–Impact–Response) and 3D modeling concerning human activity and the global warming effects. In its history Paprocany reservoir overcame numerous hydrotechnical changes influencing its present functioning. Also, its current state is significantly influenced by saline water from the coal mine (5 g L−1 of chlorides and sulphates) and biogenic elements in recreational area (about 70 mg L−1 of chlorate and to 1.9 mg L−1 Kjeldahl nitrogen) and in sediments (222.66 Mg of Kjeldahl nitrogen; 45.65 Mg of P; and 1.03 Mg of assimilable phosphorus). Concluding, the best solutions to improve the Paprocany reservoir water quality comprise: increasing alimentation with water and shortening the water exchange time, restoration of the 19th century water treatment plant, and wetlands and reed bed area revitalization. The study also proved the applicability of mathematical models in planning of the actions and anticipating their efficiency. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84978481147,

title = {A modified weighted mixture model for the interpretation of spatial and temporal changes in the microbial communities in drinking water reservoirs using compositional phospholipid fatty acid data},

author = { I. Stanimirova and A. Woźnica and T. Płociniczak and M. Kwaśniewski and J. Karczewski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978481147&doi=10.1016%2fj.talanta.2016.07.006&partnerID=40&md5=e0f69a1e3ca875379b031e8d2d369b65},

doi = {10.1016/j.talanta.2016.07.006},

issn = {00399140},

year  = {2016},

date = {2016-01-01},

journal = {Talanta},

volume = {160},

pages = {148-156},

publisher = {Elsevier B.V.},

abstract = {The aim of this work was to check whether a methodology based on the analysis of data that contain the entire phospholipid fatty acid, PLFA, compositions of water samples can be successfully used to interpret spatial and temporal changes in the microbial communities in water reservoirs. The proposed methodology consists of the construction of a modified weighted multivariate mixture model for the PLFA profiles of the water samples collected in a given monitoring campaign and the identification of latent PLFA components through a comparison with the known PLFA profiles of some cultivated or non-cultivated microbial communities. A 16S rDNA analysis of some of the selected water samples in the monitoring campaign was performed in order to verify the results of the PLFA analysis. The results showed that the proposed methodology can be useful for a dynamic and sensitive evaluation of changes in the microbial quality of water before and after flash flooding and can help in taking a decision regarding further risk assessment. © 2016 Elsevier B.V.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84992695558,

title = {Bioaccumulation of heavy metals in selected organs of black locust (Robinia pseudoacacia) and their potential use as air contamination bioindicators},

author = { B.F. Palowski and E. Małkowska and R. Kurtyka and J. Szymanowska-Pułka and E. Gucwa-Przepióra and Ł. Małkowski and A. Woźnica and E. Małkowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84992695558&doi=10.15244%2fpjoes%2f62641&partnerID=40&md5=3b5d4f2962a2474fd71235d35cb20d61},

doi = {10.15244/pjoes/62641},

issn = {12301485},

year  = {2016},

date = {2016-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {25},

number = {5},

pages = {2085-2096},

publisher = {HARD Publishing Company},

abstract = {We analyzed concentrations of Cd, Cu, Fe, Pb, and Zn in bark, leaves, young shoots, pericarp, and seeds of Robinia pseudoacacia from several sites (three industrial cities and two rural villages) in southern Poland. The objective of the study was to determine the possible usefulness of various organs and tissues of this species for biomonitoring. The analyses showed that bark and leaves are organs that may serve as good bioindicators. The lowest concentrations of Cd, Pb, and Zn were found in one of the industrial cities in the area. On the other hand, the concentrations of Cd, Pb, and Zn were similar in the rural areas when compared to the concentrations observed in the cities. The high level of metal contamination of air in rural areas may be a result of the long-range transport of emissions. Also, the use of bark and leaves of R. pseudoacacia enables us to estimate the level of contamination by Fe and Cu of areas in the vicinity of railway tracks. On the basis of the data presented in this paper and the data from references, a conclusion can be drawn that the bark and leaves of R. pseudoacacia are good bioindicators of metal contamination of air in towns and cities with different traffic intensities and within the surroundings of industrial plants and railway tracks. R. pseudoacacia was also used for the first time to assess the long-range transport of emissions. © 2016, HARD Publishing Company. All rights reserved.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84927722789,

title = {SEM-EDS and X-ray micro computed tomography studies of skeletal surface pattern and body structure in the freshwater sponge Spongilla lacustris collected from Goczalkowice reservoir habit (Southern Poland)},

author = { J. Karcz and A. Woźnica and M. Binkowski and M. Klonowska-Olejnik and T. Bernaś and J. Karczewski and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84927722789&doi=10.5603%2fFHC.a2015.0002&partnerID=40&md5=bbf1fb0ce9671ccffdc382e47d994831},

doi = {10.5603/FHC.a2015.0002},

issn = {02398508},

year  = {2015},

date = {2015-01-01},

journal = {Folia Histochemica et Cytobiologica},

volume = {53},

number = {1},

pages = {88-95},

publisher = {Via Medica},

abstract = {Introduction. Freshwater sponges are common animals of most aquatic ecosystems. They feed by filtering small particles from the water, and so are thought to be sensitive indicators of pollution. Sponges are strongly associated with the abiotic environment and are therefore used as bioindicators for monitoring of water quality in water habitats. Among the freshwater sponges, Spongilla lacustris is one of the classic models used to study evolution, gene regulation, development, physiology and structural biology in animal water systems. It is also important in diagnostic of aquatic environments. The aim of this study was to characterize and visualize three-dimensional architecture of sponge body and measure skeleton elements of S. lacustris from Goczalkowice reservoir for identification purposes. Material and methods. The scanning electron microscopy with an energy dispersive X-ray microanalysis (SEM- -EDS) and X-ray micro computed tomography (micro-CT) were used to provide non-invasive visualization of the three-dimensional architecture of Spongilla lacustris body. Results. We showed that sponge skeleton was not homogeneous in composition and comprised several forms of skeleton organization. Ectosomal skeleton occurred as spicular brushes at apices of primary fibres with cementing spongin material. Choanosomal skeletal architecture was alveolate with pauci- to multispicular primary fibres connected by paucispicular transverse fibres, made by megascleres embedded in a scanty spongin matrix both in the choanosome and at the sponge surface. In contrast, microscleres were irregularly scattered in choanosome and skeletal surface. Furthermore, SEM-EDS studies showed that the distribution of silica in megascleres and microscleres was observed along the spicules and sponge surface areas. Conclusions. In conclusion, we showed that the combination of SEM-EDS and micro-CT microscopy techniques allowed obtaining a complete picture of the sponge spatial architecture. © Polish Society for Histochemistry and Cytochemistry.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84923343076,

title = {Impact of waterbirds on chemical and biological features of water and sediments of a large, shallow dam reservoir},

author = { R. Gwiazda and A. Woźnica and B. Łozowski and M. Kostecki and A. Flis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923343076&doi=10.2478%2fs13545-014-0160-9&partnerID=40&md5=ceb35fb6544c53cfca41fc0075a6f333},

doi = {10.2478/s13545-014-0160-9},

issn = {1730413X},

year  = {2014},

date = {2014-01-01},

journal = {Oceanological and Hydrobiological Studies},

volume = {43},

number = {4},

pages = {418-426},

publisher = {Walter de Gruyter GmbH},

abstract = {Large numbers of Mallard Anas platyrhynchos (max. 10;490 ind.), Black-headed Gull Chroicocephalus ridibundus (max. 3;430 ind.) and Great Cormorant Phalacrocorax carbo (max. 1;449 ind.) were recorded on the Goczakowice Reservoir, Poland (2;754 ha). Most of the waterbirds occurred in the backwater of this reservoir. The amount of phosphorus and nitrogen loaded by the most numerous waterbirds into Goczakowice Reservoir was estimated at 958 kg and 2,621 kg, respectively in 2011 and 1,043 kg and 2,793 kg, respectively in 2012. In 2011 and 2012, the waterbirds introduced a considerable amount of phosphorus, nitrogen and a large number of coliforms into the backwater of the reservoir. The concentration of different forms of phosphorus and nitrogen, chlorophyll-a and bacteria coli in the water was not greater at the site of birds' concentration (except dissolved organic nitrogen). The concentration of nitrates in the water at the site near the breeding colony of gulls in comparison with the reference site was not different. The amounts of P-tot and N-tot in the sediment were similar at the site affected by waterbirds and at the reference site. The dynamics of water masses was not the reason for the lack of differences between the studied sites. Copyright © of Faculty of Oceanography and Geography .},

note = {21},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84880418821,

title = {Mobile sailing robot for automatic estimation of fish density and monitoring water quality},

author = { R. Koprowski and Z. Wróbel and A. Kleszcz and S. Wilczyński and A. Woźnica and B. Łozowski and M. Pilarczyk and J. Karczewski and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84880418821&doi=10.1186%2f1475-925X-12-60&partnerID=40&md5=7f25d9525b41d51dea739062a7e69c2c},

doi = {10.1186/1475-925X-12-60},

issn = {1475925X},

year  = {2013},

date = {2013-01-01},

journal = {BioMedical Engineering Online},

volume = {12},

number = {1},

publisher = {BioMed Central Ltd.},

abstract = {Introduction: The paper presents the methodology and the algorithm developed to analyze sonar images focused on fish detection in small water bodies and measurement of their parameters: volume, depth and the GPS location. The final results are stored in a table and can be exported to any numerical environment for further analysis.Material and method: The measurement method for estimating the number of fish using the automatic robot is based on a sequential calculation of the number of occurrences of fish on the set trajectory. The data analysis from the sonar concerned automatic recognition of fish using the methods of image analysis and processing.Results: Image analysis algorithm, a mobile robot together with its control in the 2.4 GHz band and full cryptographic communication with the data archiving station was developed as part of this study. For the three model fish ponds where verification of fish catches was carried out (548; 171 and 226 individuals), the measurement error for the described method was not exceeded 8%.Summary: Created robot together with the developed software has features for remote work also in the variety of harsh weather and environmental conditions, is fully automated and can be remotely controlled using Internet. Designed system enables fish spatial location (GPS coordinates and the depth). The purpose of the robot is a non-invasive measurement of the number of fish in water reservoirs and a measurement of the quality of drinking water consumed by humans, especially in situations where local sources of pollution could have a significant impact on the quality of water collected for water treatment for people and when getting to these places is difficult. The systematically used robot equipped with the appropriate sensors, can be part of early warning system against the pollution of water used by humans (drinking water; natural swimming pools) which can be dangerous for their health. © 2013 Koprowski et al.; licensee BioMed Central Ltd.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84872239075,

title = {Stimulatory Effect of Xenobiotics on Oxidative Electron Transport of Chemolithotrophic Nitrifying Bacteria Used as Biosensing Element},

author = { A. Woźnica and A. Nowak and P. Ziemski and M. Kwaśniewski and T. Bernaś},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84872239075&doi=10.1371%2fjournal.pone.0053484&partnerID=40&md5=02b3ae1029cc57b8d38913093924c259},

doi = {10.1371/journal.pone.0053484},

issn = {19326203},

year  = {2013},

date = {2013-01-01},

journal = {PLoS ONE},

volume = {8},

number = {1},

abstract = {Electron transport chain (ETCh) of ammonium (AOB) and nitrite oxidizing bacteria (NOB) participates in oxidation of ammonium to nitrate (nitrification). Operation of ETCh may be perturbed by a range of water-soluble xenobiotics. Therefore, consortia of nitrifying bacteria may be used as a biosensor to detect water contamination. A surprising feature of this system is an increase of oxygen consumption, detected in the presence of certain inhibitors of ETCh. Thus, to shed light on the mechanism of this effect (and other differences between inhibitors) we monitored separately respiration of the bacteria of the first (AOB - Nitrosomonas) and second (NOB -Nitrobacter) stages of nitrification. Furthermore, we measured plasma membrane potential and the level of reduction of NAD(P)H. We propose a novel model of ETCh in NOB to explain the role of reverse electron transport in the stimulation of oxygen consumption (previously attributed to hormesis). © 2013 Woznica et al.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84860400082,

title = {Application of lyophilization to prepare the nitrifying bacterial biofilm for imaging with scanning electron microscopy},

author = { J. Karcz and T. Bernaś and A. Nowak and E. Talik and A. Woźnica},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860400082&doi=10.1002%2fsca.20275&partnerID=40&md5=bd7759b33a3bb5d23d784ce4f1a8e42a},

doi = {10.1002/sca.20275},

issn = {01610457},

year  = {2012},

date = {2012-01-01},

journal = {Scanning},

volume = {34},

number = {1},

pages = {26-36},

abstract = {Structure of bacterial biofilms may be investigated using several variants of scanning electron microscopy (SEM). We apply lyophilization to prepare nitrifying bacterial biofilm for conventional SEM imaging in high-vacuum mode (CSEM). We therefore replace standard biofilm fixation in glutaraldehyde cross-linking, ethanol dehydration, and critical-point drying (CPD) with less-invasive low-temperature drying by sublimation in vacuum. We compare this approach with: (1) standard preparation with glutaraldehyde fixation, ethanol dehydration, and CPD before CSEM, (2) cryo-sputter preparation of rapidly frozen biofilm in hydrated state (cryo-SEM), and (3) in situ observation without any sample pretreatment in environmental SEM. Combined imaging with these modalities revealed two distinct immobilization patterns on the polyurethane foam: (1) large irregular aggregates (flocs) of bacterial biofilm that exist as irregular biofilm fragments, rope-like structures, or biofilm layers on the foam surface; (2) biofilm threads adherent to the surface of polyurethane foam. Our results indicate that lyophilization was suitable for preservation of bacterial cells and many forms of structure of extracellular matrix. The lyophilized material could be imaged with high resolution (using CSEM) to generate structural information complementary to that obtained with other SEM techniques. © 2011 Wiley Periodicals, Inc.},

note = {34},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79451475692,

title = {Spatial architecture of nitrifying bacteria biofilm immobilized on polyurethane foam in an automatic biodetector for water toxicity},

author = { A. Woźnica and J. Karcz and A. Nowak and A. Gmur and T. Bernaś},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79451475692&doi=10.1017%2fS1431927610093815&partnerID=40&md5=603a0cfce59326febfa6b79789f382c7},

doi = {10.1017/S1431927610093815},

issn = {14319276},

year  = {2010},

date = {2010-01-01},

journal = {Microscopy and Microanalysis},

volume = {16},

number = {5},

pages = {550-560},

abstract = {We describe the architecture of nitrifying bacteria biofilms immobilized on a three-dimensional (3D) polyurethane foam that permits efficient water flow through a bioreactor. The 3D spatial organization of immobilized bacterial colonies is characterized on three resolution levels with X-ray tomography, light confocal microscopy, and scanning electron microscopy (SEM). Using these techniques we demonstrate biofilm distribution in the foam and the existence of several modes of binding of bacteria to the foam. Computed X-ray tomography permits observation of the distribution of the biofilm in the whole open cellular polyurethane material volume and estimation of biofilm volume. SEM and confocal laser scanning microscopy techniques permit 3D visualization of biofilm structure. Three distinct immobilization patterns could be observed in the open cellular polyurethane material: (1) large irregular aggregates of bacterial biofilm that exist as irregular biofilm fragments, rope-like structures, or biofilm layers on the foam surface; (2) spherical (pom-pom) aggregates of bacteria localized on the external surface of biofilm; and (3) biofilm threads adherent to the surface of polyurethane foam. Finally, we demonstrate that immobilized bacteria exhibit metabolic activity and growth. © Microscopy Society of America 2010.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77957239169,

title = {Automatic biodetector of water toxicity (ABTOW) as a tool for examination of phenol and cyanide contaminated water},

author = { A. Woźnica and A. Nowak and J. Karczewski and C. Kliś and T. Bernaś},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957239169&doi=10.1016%2fj.chemosphere.2010.07.011&partnerID=40&md5=46fe6a643fb051511c387cd40d5e4a5a},

doi = {10.1016/j.chemosphere.2010.07.011},

issn = {00456535},

year  = {2010},

date = {2010-01-01},

journal = {Chemosphere},

volume = {81},

number = {6},

pages = {767-772},

publisher = {Elsevier Ltd},

abstract = {We describe an automatic biodetector for continuous monitoring of water toxicity (ABTOW). Construction of the ABTOW is based on natural ability of the biofilm formation to immobilize consortia of nitrifying bacteria (the sensing element) on the open cellular polyurethane foam as the support. Change of rates of oxygen consumption is used as an indicator of biocatalytic activity (nitrification) of the bacteria in response to xenobiotics. Owing to this design the ABTOW features stability long-term use, is inexpensive and simple in operation. The dynamics of ABTOW response is studied in details for phenol and cyanide as model toxins. These data indicate that the sensitivity was 3.5μM for phenol and 0.19μM for cyanide, respectively. The magnitudes of toxic effect were proportional to concentration whereas kinetics of the response is an indicator for the mechanism of toxicity. Similar methodology is applied to quantify toxicity of a range of heavy metals, herbicides and oxidative chain inhibitors. One may conclude that the presented biodetector provides a good sensitivity for continuous on-line monitoring of toxicity in water. © 2010 Elsevier Ltd.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-75449120080,

title = {Monitoring structure and activity of nitrifying bacterial biofilm in an automatic biodetector of water toxicity},

author = { A. Woźnica and A. Nowak and C. Beimfohr and J. Karczewski and T. Bernaś},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-75449120080&doi=10.1016%2fj.chemosphere.2009.12.035&partnerID=40&md5=7d9825b9b731fb593461d73a8f72643f},

doi = {10.1016/j.chemosphere.2009.12.035},

issn = {00456535},

year  = {2010},

date = {2010-01-01},

journal = {Chemosphere},

volume = {78},

number = {9},

pages = {1121-1128},

publisher = {Elsevier Ltd},

abstract = {Automatic biodetector of water toxicity is a biosensor based on monitoring of catalytic activity of the nitrifying bacteria. To create a standardized biosensing system, development of the biofilm must be characterized to determine the prerequisites for its biological (biocatalytic) stability. In this paper, growth of biofilm comprising ammonium-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the open cellular polyurethane material polyurethane sponge bioreactor has been investigated. Dynamics of the biofilm formation was estimated using AOB and NOB metabolic activity and the volume occupied by these two types of bacteria in the biofilm. Spectrophotometry liquid ion chromatography and image cytometry were used, respectively, for these measurements. A mathematical model of the dynamics of biofilm formation was established. These data indicate that open cellular polyurethane material is a good basis for the immobilization of nitrifying bacteria. Moreover, growth of the biofilm leads to its stable structural form, whose biocatalytic activity (12.29 for AOB and 6.84 μmol min-1 for NOB) is constant in the long term. © 2009 Elsevier Ltd. All rights reserved.},

note = {13},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0041659314,

title = {Effects of electron transport inhibitors on iron reduction in Aeromonas hydrophila strain KB1},

author = { A. Woźnica and J. Dzirba and D. Mańka and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041659314&doi=10.1016%2fS1075-9964%2803%2900059-3&partnerID=40&md5=bbc75712c0af653bb2ca5fe286226785},

doi = {10.1016/S1075-9964(03)00059-3},

issn = {10759964},

year  = {2003},

date = {2003-01-01},

journal = {Anaerobe},

volume = {9},

number = {3},

pages = {125-130},

publisher = {Academic Press},

abstract = {The aim of this study was to determine the influence of respiratory chain inhibitors upon iron (III) reduction in Aeromonas hydrophila strain KB1. Optimal conditions of the reduction process were established by determining the amount of biomass, optimal pH, temperature and substrate concentration. The obtained results allowed us to determine Hill equation coefficients (K m=1.45±0.18mM; Vmax=83.40±2.70μM/min, and h=0.7±0.03). The value of h points to Michaelis-like kinetics of the process. The substrate concentration used in our study was such as to allow the maximum iron reduction rate. The reaction was mesophilic. The participation of electron carriers in the iron reduction process was investigated using respiratory chain inhibitors. Rotenone and capsaicin were used to study Q sites of the respiratory chain complex I. Dicumarol was used as an inhibitor of the quinone loop, while quinacrine was used to inhibit alloxazine centers. Additionally, complex III inhibitors, such as antimycin A, myxothiazole and 2-heptyl-4-hydroxy-quinoline N-oxide (HQNO) were used. Azide was used to inhibit complex IV. The observed inhibition of iron reduction by rotenone and capsaicin may suggest the existence of Q sites in formate reductase, analogous to those in complex I. Inhibition of quinones, isoalloxazine centers and complex III suggests participation of these carriers in the electron transport during iron reduction. Lack of inhibition of iron reduction by azide suggests that complex IV does not participate in this process. © 2003 Elsevier Ltd. All rights reserved.},

note = {27},

keywords = {},

pubstate = {published},

tppubtype = {article}

}