• mgr Ilona Witas
Stanowisko: Specj.inż-tech.
Jednostka: Biuro ds. Infrastruktury Badawczo-Dydaktycznej WNP
Adres: 40-007 Katowice, ul. Bankowa 9
Piętro: II
Numer pokoju: 218
Telefon: (32) 359 1728
E-mail: ilona.witas@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 6507837733
Publikacje z bazy Scopus
2011
Babczyńska, A.; Wilczek, G.; Wilczek, P.; Szulińska, E.; Witas, I.
In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, vol. 154, no. 3, pp. 161-171, 2011, ISSN: 15320456, (33).
@article{2-s2.0-79960955274,
title = {Metallothioneins and energy budget indices in cadmium and copper exposed spiders Agelena labyrinthica in relation to their developmental stage, gender and origin},
author = { A. Babczyńska and G. Wilczek and P. Wilczek and E. Szulińska and I. Witas},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960955274&doi=10.1016%2fj.cbpc.2011.05.001&partnerID=40&md5=48cd98709a9efeedcae519104b3090ec},
doi = {10.1016/j.cbpc.2011.05.001},
issn = {15320456},
year = {2011},
date = {2011-01-01},
journal = {Comparative Biochemistry and Physiology - C Toxicology and Pharmacology},
volume = {154},
number = {3},
pages = {161-171},
publisher = {Elsevier Inc.},
abstract = {The aim of our studies was to explain the role of metallothioneins (MTs) in the neutralization of excessive amounts of metals (essential: copper (Cu) and toxic: cadmium (Cd)) and to describe the energy status in metal-exposed spiders Agelena labyrinthica in relation to its developmental stage, gender and origin. Juvenile, female and male spiders were collected from three variously polluted habitats, transferred to the laboratory and exposed to the metals in their diet. Cu and Cd accumulation in the body and exuviae, bioaccumulation factor, percentage of metallothionein positive cells, MT concentration, percentage of cells with depolarized mitochondria, ATP concentration and ADP/ATP ratio were measured and calculated. Cu appeared to be regulated and its excess is eliminated via, among others, the molting process, while Cd was rather accumulated by the spiders. The level of MTs increased significantly mainly in females exposed to both metals, irrespectively of the pollution degree of their site of origin, indicating a defensive role of the proteins. In general, even if both the MT level and the energy status indices were positively correlated with Cd and Cu concentrations in the spider body, the energy status of A. labyrinthica did not seem disturbed. © 2011 Elsevier Inc.},
note = {33},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of our studies was to explain the role of metallothioneins (MTs) in the neutralization of excessive amounts of metals (essential: copper (Cu) and toxic: cadmium (Cd)) and to describe the energy status in metal-exposed spiders Agelena labyrinthica in relation to its developmental stage, gender and origin. Juvenile, female and male spiders were collected from three variously polluted habitats, transferred to the laboratory and exposed to the metals in their diet. Cu and Cd accumulation in the body and exuviae, bioaccumulation factor, percentage of metallothionein positive cells, MT concentration, percentage of cells with depolarized mitochondria, ATP concentration and ADP/ATP ratio were measured and calculated. Cu appeared to be regulated and its excess is eliminated via, among others, the molting process, while Cd was rather accumulated by the spiders. The level of MTs increased significantly mainly in females exposed to both metals, irrespectively of the pollution degree of their site of origin, indicating a defensive role of the proteins. In general, even if both the MT level and the energy status indices were positively correlated with Cd and Cu concentrations in the spider body, the energy status of A. labyrinthica did not seem disturbed. © 2011 Elsevier Inc.
2004
Łaszczyca, P.; Augustyniak, M.; Babczyńska, A.; Bednarska, K.; Kafel, A.; Migula, P.; Wilczek, G.; Witas, I.
Profiles of enzymatic activity in earthworms from zinc, lead and cadmium polluted areas near Olkusz (Poland) Journal Article
In: Environment International, vol. 30, no. 7, pp. 901-910, 2004, ISSN: 01604120, (113).
@article{2-s2.0-2942670066,
title = {Profiles of enzymatic activity in earthworms from zinc, lead and cadmium polluted areas near Olkusz (Poland)},
author = { P. Łaszczyca and M. Augustyniak and A. Babczyńska and K. Bednarska and A. Kafel and P. Migula and G. Wilczek and I. Witas},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2942670066&doi=10.1016%2fj.envint.2004.02.006&partnerID=40&md5=fb1bf084ee1cffc308a2059a62a4a95c},
doi = {10.1016/j.envint.2004.02.006},
issn = {01604120},
year = {2004},
date = {2004-01-01},
journal = {Environment International},
volume = {30},
number = {7},
pages = {901-910},
publisher = {Elsevier Ltd},
abstract = {The aim of the study was to determine whether there are signs of adaptation of soil fauna to a gradient of heavy metal contamination. Earthworms Aporrectodea caliginosa, Lumbricus terrestris and Eisenia fetida were collected during the spring and summer of 2000 and 2001 from meadow sites situated between 2 and 32 km from the Bukowno-Olkusz complex of zinc-lead ore mines and smelters. The heavy metal content in the soil near smelters reaches 10,500 mg/kg (d.w.) for Zn, 2600 mg/kg for Pb and 81.9 mg/kg for Cd. The sites differ with respect to species composition of earthworm community, with A. caliginosa being dominant. Complete data was obtained only for A. caliginosa, since other species were not abundant at all investigated sites during the whole period of investigation. The body burdens of Zn, Pb, Cd and Cu in A. caliginosa reached 1500, 100, 220 and 10 μg/g, respectively, in the vicinity of the smelter (2-4 km), and decreased to 400, 2, 36 and 6 μg/g at the most distant site (32 km). Cadmium and lead content was significantly elevated in the whole body of L. terrestris collected at the site 2.5 km distant from the smelters when compared to more distant sites, while in E. fetida only the body burden of cadmium was elevated at the nearest site compared to the next site of transect. Activities of glutathione peroxidase (GPX; EC 1.11.1.9) against hydrogen peroxide (H2O2) or cumene hydroperoxide (cumOOH), glutathione reductase (GR; EC 1.6.4.2), glutathione S-transferase (GST; EC 2.5.1.18) and catalase (CAT; EC 1.11.1.6) were assayed in postmitochondrial supernatant obtained from whole body homogenates. Seasonal and annual variations of enzyme activity were reflected by higher GPX activity in the late summer of 2001 in comparison with the spring and summer of 2000. This may reflect severe drought in the spring and summer of 2000. The activity of both GPX isozymes, GR and GST in A. caliginosa and L. terrestris increased with increasing distance from the smelters and reached maximum at sites III and IV (4 and 8 km from the smelters; respectively) and then it decreased in the animals from site V (32 km). These may be the effects of antagonism between the enzyme inducing and enzyme inhibiting action of smelter emissions, a phenomenon known as a hormetic effect. It is postulated here that this effect is of diagnostic value for metal pollution biomonitoring. © 2004 Elsevier Ltd. All rights reserved.},
note = {113},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of the study was to determine whether there are signs of adaptation of soil fauna to a gradient of heavy metal contamination. Earthworms Aporrectodea caliginosa, Lumbricus terrestris and Eisenia fetida were collected during the spring and summer of 2000 and 2001 from meadow sites situated between 2 and 32 km from the Bukowno-Olkusz complex of zinc-lead ore mines and smelters. The heavy metal content in the soil near smelters reaches 10,500 mg/kg (d.w.) for Zn, 2600 mg/kg for Pb and 81.9 mg/kg for Cd. The sites differ with respect to species composition of earthworm community, with A. caliginosa being dominant. Complete data was obtained only for A. caliginosa, since other species were not abundant at all investigated sites during the whole period of investigation. The body burdens of Zn, Pb, Cd and Cu in A. caliginosa reached 1500, 100, 220 and 10 μg/g, respectively, in the vicinity of the smelter (2-4 km), and decreased to 400, 2, 36 and 6 μg/g at the most distant site (32 km). Cadmium and lead content was significantly elevated in the whole body of L. terrestris collected at the site 2.5 km distant from the smelters when compared to more distant sites, while in E. fetida only the body burden of cadmium was elevated at the nearest site compared to the next site of transect. Activities of glutathione peroxidase (GPX; EC 1.11.1.9) against hydrogen peroxide (H2O2) or cumene hydroperoxide (cumOOH), glutathione reductase (GR; EC 1.6.4.2), glutathione S-transferase (GST; EC 2.5.1.18) and catalase (CAT; EC 1.11.1.6) were assayed in postmitochondrial supernatant obtained from whole body homogenates. Seasonal and annual variations of enzyme activity were reflected by higher GPX activity in the late summer of 2001 in comparison with the spring and summer of 2000. This may reflect severe drought in the spring and summer of 2000. The activity of both GPX isozymes, GR and GST in A. caliginosa and L. terrestris increased with increasing distance from the smelters and reached maximum at sites III and IV (4 and 8 km from the smelters; respectively) and then it decreased in the animals from site V (32 km). These may be the effects of antagonism between the enzyme inducing and enzyme inhibiting action of smelter emissions, a phenomenon known as a hormetic effect. It is postulated here that this effect is of diagnostic value for metal pollution biomonitoring. © 2004 Elsevier Ltd. All rights reserved.
2003
Kafel, A.; Bednarska, K.; Augustyniak, M.; Witas, I.; Szulińska, E.
Activity of glutathione S-transferase in Spodoptera exigua larvae exposed to cadmium and zinc in two subsequent generations Journal Article
In: Environment International, vol. 28, no. 8, pp. 683-686, 2003, ISSN: 01604120, (23).
@article{2-s2.0-0037340581,
title = {Activity of glutathione S-transferase in Spodoptera exigua larvae exposed to cadmium and zinc in two subsequent generations},
author = { A. Kafel and K. Bednarska and M. Augustyniak and I. Witas and E. Szulińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037340581&doi=10.1016%2fS0160-4120%2802%2900111-3&partnerID=40&md5=8082a67a14755b7f7ed59ab4571f1c33},
doi = {10.1016/S0160-4120(02)00111-3},
issn = {01604120},
year = {2003},
date = {2003-01-01},
journal = {Environment International},
volume = {28},
number = {8},
pages = {683-686},
publisher = {Elsevier Ltd},
abstract = {The aim of our study was to establish changes in activity of important in detoxification enzyme - glutathione S-transferase (GST): in alimentary tract, fat body and Malpighian tubules of Spodoptera exigua larvae being under cadmium and zinc exposure through the first as well as the second generation. There was registered enhancement of the enzyme activity in the fat body and the Malpighian tubules caused by zinc as well as its decrease in the Malpighian tubules under cadmium action. Amounts of metals in the alimentary tract were either several times higher than in the diet ingested by larvae or than in the fat body. Metal concentration in the fat body correlated with the level of the enzyme activity (positive correlation for zinc and negative for cadmium). The effect of metal action differentiated dependently on time exposition. © 2002 Elsevier Science Ltd. All rights reserved.},
note = {23},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of our study was to establish changes in activity of important in detoxification enzyme - glutathione S-transferase (GST): in alimentary tract, fat body and Malpighian tubules of Spodoptera exigua larvae being under cadmium and zinc exposure through the first as well as the second generation. There was registered enhancement of the enzyme activity in the fat body and the Malpighian tubules caused by zinc as well as its decrease in the Malpighian tubules under cadmium action. Amounts of metals in the alimentary tract were either several times higher than in the diet ingested by larvae or than in the fat body. Metal concentration in the fat body correlated with the level of the enzyme activity (positive correlation for zinc and negative for cadmium). The effect of metal action differentiated dependently on time exposition. © 2002 Elsevier Science Ltd. All rights reserved.
1996
Łaszczyca, P.; Kawka-Serwecińska, E.; Witas, I.; Doleżych, B.; Falkus, B.; Mekail, A.; Ziółkowska, B.; Madej, P.; Migula, P.
Lipid peroxidation and activity of antioxidative enzymes in the rat model of ozone therapy. Journal Article
In: Materia medica Polona. Polish journal of medicine and pharmacy, vol. 28, no. 4, pp. 155-160, 1996, ISSN: 00255246, (8).
@article{2-s2.0-0030252327,
title = {Lipid peroxidation and activity of antioxidative enzymes in the rat model of ozone therapy.},
author = { P. Łaszczyca and E. Kawka-Serwecińska and I. Witas and B. Doleżych and B. Falkus and A. Mekail and B. Ziółkowska and P. Madej and P. Migula},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030252327&partnerID=40&md5=f6a9c197867425e982bec688275688d5},
issn = {00255246},
year = {1996},
date = {1996-01-01},
journal = {Materia medica Polona. Polish journal of medicine and pharmacy},
volume = {28},
number = {4},
pages = {155-160},
abstract = {Hypothetical, therapeutic effects of ozone were investigated in an animal model. One ml of oxygen or mixture of 40 micrograms ozone with oxygen were injected intraperitoneally to male rats for 10 days. Previously, rats had been poisoned with 50 ppm Cd2+ in drinking water for 12 weeks. Exhaustive treadmill running was applied to some animals before sacrification. Ozone injections increased iron-ascorbate-stimulated lipid peroxidation (LPO) in the liver and kidney, catalase (CAT) activity in the heart and glutathione S-transferase (GST) activity in the heart, kidney and liver. Oxygen increased GST activity in the brain and reduced glutathione peroxidase (GPX) activity in the kidney. Cadmium enhanced LPO in the liver and GST activity in the brain, heart, kidney and liver. In contrast to ozone, cadmium inhibited GPX activity in the brain, kidney and liver. Cadmium combined with ozone enhanced the changes of GPX activity in the kidney and liver, that of GST activity in the heart, kidney and liver as well as of CAT activity and LPO in kidney. The results suggest that ozone injections combined with tested factors may provoke an oxidative stress. The effects of ozone therapy can not be explained as the results of ozone action on the antioxidative enzymes in rat.},
note = {8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Hypothetical, therapeutic effects of ozone were investigated in an animal model. One ml of oxygen or mixture of 40 micrograms ozone with oxygen were injected intraperitoneally to male rats for 10 days. Previously, rats had been poisoned with 50 ppm Cd2+ in drinking water for 12 weeks. Exhaustive treadmill running was applied to some animals before sacrification. Ozone injections increased iron-ascorbate-stimulated lipid peroxidation (LPO) in the liver and kidney, catalase (CAT) activity in the heart and glutathione S-transferase (GST) activity in the heart, kidney and liver. Oxygen increased GST activity in the brain and reduced glutathione peroxidase (GPX) activity in the kidney. Cadmium enhanced LPO in the liver and GST activity in the brain, heart, kidney and liver. In contrast to ozone, cadmium inhibited GPX activity in the brain, kidney and liver. Cadmium combined with ozone enhanced the changes of GPX activity in the kidney and liver, that of GST activity in the heart, kidney and liver as well as of CAT activity and LPO in kidney. The results suggest that ozone injections combined with tested factors may provoke an oxidative stress. The effects of ozone therapy can not be explained as the results of ozone action on the antioxidative enzymes in rat.
1995
Łaszczyca, P.; Kawka-Serwecińska, E.; Witas, I.; Doleżych, B.; Migula, P.
Iron ascorbate-stimulated lipid peroxidation in vitro. Why is the method controversial? Journal Article
In: General Physiology and Biophysics, vol. 14, no. 1, pp. 3-18, 1995, ISSN: 02315882, (5).
@article{2-s2.0-0029014509,
title = {Iron ascorbate-stimulated lipid peroxidation in vitro. Why is the method controversial?},
author = { P. Łaszczyca and E. Kawka-Serwecińska and I. Witas and B. Doleżych and P. Migula},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029014509&partnerID=40&md5=cae792886345504ee1224eda707729d2},
issn = {02315882},
year = {1995},
date = {1995-01-01},
journal = {General Physiology and Biophysics},
volume = {14},
number = {1},
pages = {3-18},
abstract = {In vitro generation of thiobarbituric acid reactive substances (TBARS) is frequently used to assess organ susceptibility to lipid peroxidation. The yield of TBARS is severalfold enhanced by an addition of iron ions with reductors or chelators such as ascorbate, NADPH, ADP or pyrophosphate. The process cannot be interpreted in a simple way, since it involves several enzymatic and nonenzymatic reactions. There are no clear interpretations of the ambiguous effects of denaturating factors and chelating agents on TBARS generation. Also controversy arises from the cirvilinear relationship between the homogenate concentration and the yield of TBARS. This has been modelled in the present work by combining two functions describing the sequential reaction with two limiting steps. One of them is related to catalytic action of iron and ascorbate, while the other to an enzyme, possibly phospholipase A2, as has been suggested by some investigators. Two models should be considered since it is impossible to decide which kinetic equation should predominate in the model. Nevertheless, the model reflects kinetic properties of the process. The effects of catalyst concentration and some other modification upon the yield of TBARS were also investigated experimentally. The results of experiments and modelling showed that the analytical procedures used by investigators need standardisation as the results obtained under a variety of procedures may reflect quite different properties of the living systems.},
note = {5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In vitro generation of thiobarbituric acid reactive substances (TBARS) is frequently used to assess organ susceptibility to lipid peroxidation. The yield of TBARS is severalfold enhanced by an addition of iron ions with reductors or chelators such as ascorbate, NADPH, ADP or pyrophosphate. The process cannot be interpreted in a simple way, since it involves several enzymatic and nonenzymatic reactions. There are no clear interpretations of the ambiguous effects of denaturating factors and chelating agents on TBARS generation. Also controversy arises from the cirvilinear relationship between the homogenate concentration and the yield of TBARS. This has been modelled in the present work by combining two functions describing the sequential reaction with two limiting steps. One of them is related to catalytic action of iron and ascorbate, while the other to an enzyme, possibly phospholipase A2, as has been suggested by some investigators. Two models should be considered since it is impossible to decide which kinetic equation should predominate in the model. Nevertheless, the model reflects kinetic properties of the process. The effects of catalyst concentration and some other modification upon the yield of TBARS were also investigated experimentally. The results of experiments and modelling showed that the analytical procedures used by investigators need standardisation as the results obtained under a variety of procedures may reflect quite different properties of the living systems.