@article{2-s2.0-85178151628,

title = {Survival, growth and digestive functions after exposure to nanodiamonds - Transgenerational effects beyond contact time in house cricket strains},

author = { M. Augustyniak and A.K. Ajay and A. Kędziorski and M. Tarnawska and M.M. Rost-Roszkowska and B. Flasz and A. Babczyńska and B. Mazur and K. Rozpędek and R.S. Alian and M. Skowronek and E. Świerczek and Kl. Wiśniewska and P. Ziętara},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85178151628&doi=10.1016%2fj.chemosphere.2023.140809&partnerID=40&md5=8596e027ee47c37b61120de37d91f704},

doi = {10.1016/j.chemosphere.2023.140809},

issn = {00456535},

year  = {2024},

date = {2024-01-01},

journal = {Chemosphere},

volume = {349},

publisher = {Elsevier Ltd},

abstract = {The long-term exposure effects of nanodiamonds (NDs), spanning an organism's entire lifespan and continuing for subsequent generation, remain understudied. Most research has focused on evaluating their biological impacts on cell lines and selected organisms, typically over short exposure durations lasting hours or days. The study aimed to assess growth, mortality, and digestive functions in wild (H) and long-lived (D) strains of Acheta domesticus (Insecta: Orthoptera) after two-generational exposure to NDs in concentrations of 0.2 or 2 mg kg−1 of food, followed by their elimination in the third generation. NDs induced subtle stimulating effect that depended on the strain and generation. In the first generation, more such responses occurred in the H than in the D strain. In the first generation of H strain insects, contact with NDs increased survival, stimulated the growth of young larvae, and the activity of most digestive enzymes in mature adults. The same doses and exposure time did not cause similar effects in the D strain. In the first generation of D strain insects, survival and growth were unaffected by NDs, whereas, in the second generation, significant stimulation of those parameters was visible. Selection towards longevity appears to support higher resistance of the insects to exposure to additional stressor, at least in the first generation. The cessation of ND exposure in the third generation caused potentially harmful changes, which included, e.g., decreased survival probability in H strain insects, slowed growth of both strains, as well as changes in heterochromatin density and distribution in nuclei of the gut cells in both strains. Such a reaction may suggest the involvement of epigenetic inheritance mechanisms, which may become inadequate after the stress factor is removed. © 2023 Elsevier Ltd},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85180363492,

title = {Adaptation by death? Cell death-based tolerance to cadmium in 150-generation exposure of Spodoptera exiqua Hübner (Lepidoptera: Noctuidae)},

author = { A. Babczyńska and M.M. Rost-Roszkowska and A. Kafel and B. Łozowski and M. Augustyniak and M. Tarnawska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85180363492&doi=10.1093%2fee%2fnvad077&partnerID=40&md5=65b08164a16d96d7682214b4d1536977},

doi = {10.1093/ee/nvad077},

issn = {0046225X},

year  = {2023},

date = {2023-01-01},

journal = {Environmental Entomology},

volume = {52},

number = {6},

pages = {1057-1070},

publisher = {Entomological Society of America},

abstract = {Mechanisms, including autophagy and apoptosis, which serve to regulate and ensure proper organism functions under optimal conditions, play additional defensive roles under environmental pressure.The aim of this study was to test the following hypotheses: (i) elevated autophagy and apoptosis intensity levels, as defensive processes in response to contact with cadmium, are maintained for a limited number of generations and (ii) the number of generations after which levels of cell death processes reach the reference level depends on selective pressure. Cell death processes were assessed by light and transmission electron microscopy, terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL), and cytometric analyses. Model insects (Spodoptera exiqua; Hübner; 1808) were orally exposed to various concentrations of cadmium for 18 generations and compared with reference strains exposed to cadmium or not (control) for over 150 generations. Elevated programmed cell death intensity levels decreased after several generations, indicating tolerance of individuals to cadmium in the diet and verifying the first hypothesis; however, testing the second hypothesis indicated that the number of generations depended not only on pressure intensity, but also on cell death type, since levels of autophagy remained increased for a minimum of 12 generations. © The Author(s) 2023. Published by Oxford University Press on behalf of Entomological Society of America.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85169134510,

title = {Multigenerational Effects of Graphene Oxide Nanoparticles on Acheta domesticus DNA Stability},

author = { B. Flasz and A.K. Ajay and M. Tarnawska and A. Babczyńska and Ł. Majchrzycki and A. Kędziorski and Ł. Napora-Rutkowski and E. Świerczek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85169134510&doi=10.3390%2fijms241612826&partnerID=40&md5=94952f38d3a2995d15e764932e1dfb79},

doi = {10.3390/ijms241612826},

issn = {16616596},

year  = {2023},

date = {2023-01-01},

journal = {International Journal of Molecular Sciences},

volume = {24},

number = {16},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {The use of nanoparticles like graphene oxide (GO) in nanocomposite industries is growing very fast. There is a strong concern that GO can enter the environment and become nanopollutatnt. Environmental pollutants’ exposure usually relates to low concentrations but may last for a long time and impact following generations. Attention should be paid to the effects of nanoparticles, especially on the DNA stability passed on to the offspring. We investigated the multigenerational effects on two strains (wild and long-lived) of house cricket intoxicated with low GO concentrations over five generations, followed by one recovery generation. Our investigation focused on oxidative stress parameters, specifically AP sites (apurinic/apyrimidinic sites) and 8-OHdG (8-hydroxy-2′-deoxyguanosine), and examined the global DNA methylation pattern. Five intoxicated generations were able to overcome the oxidative stress, showing that relatively low doses of GO have a moderate effect on the house cricket (8-OHdG and AP sites). The last recovery generation that experienced a transition from contaminated to uncontaminated food presented greater DNA damage. The pattern of DNA methylation was comparable in every generation, suggesting that other epigenetic mechanisms might be involved. © 2023 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85164355155,

title = {Joint cadmium and polypropylene microparticle action in cadmium tolerant model insect},

author = { A. Babczyńska and Mi. Górka and A. Lis and M. Tarnawska and B. Łozowski and J. Brożek and K. Rozpędek and M. Augustyniak and M. Skowronek and A. Kafel},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85164355155&doi=10.1016%2fj.etap.2023.104209&partnerID=40&md5=fd1f5028f306c019e8d9e70db430f033},

doi = {10.1016/j.etap.2023.104209},

issn = {13826689},

year  = {2023},

date = {2023-01-01},

journal = {Environmental Toxicology and Pharmacology},

volume = {101},

publisher = {Elsevier B.V.},

abstract = {Microplastic enlisted as a contaminant of emerging concerns in polluted environments interact with “traditional” contaminants such as metals, causing, among others, their increased accumulation in the body. Harmful effects depend on the exposed animals' possible preadaptation and/or cross-tolerance. The project aimed to assess the role of this phenomenon in the limited toxicity of polypropylene fibers (PPf) in 0%, 0.02%, 0.06, 0.18%, 0.54%, and 1.6% of Cd-supplemented food of larvae of Spodoptera exigua multigenerationally selected to cadmium tolerance. The activity of 20 digestive enzymes (API-ZYM test), defensins, and heat shock proteins, HSP70 levels in the exposed groups were used as biomarkers. PPfs caused the increase of Cd accumulation in the body, while intake of polypropylene microfibers did not change the biomarker levels. Moreover, multigenerational Cd pre-exposure, due to increased tolerance of Cd and, possibly, cross-tolerance, prepares the insects for an additional stressor (PPf) alone and in interaction with cadmium. © 2023 Elsevier B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85145976280,

title = {Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus},

author = { B. Flasz and M. Dziewięcka and A.K. Ajay and M. Tarnawska and A. Babczyńska and A. Kędziorski and Ł. Napora-Rutkowski and P. Ziętara and E. Świerczek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145976280&doi=10.3390%2fijms24010290&partnerID=40&md5=5d497b4e9e8b4082341d87d5be39d3d6},

doi = {10.3390/ijms24010290},

issn = {16616596},

year  = {2023},

date = {2023-01-01},

journal = {International Journal of Molecular Sciences},

volume = {24},

number = {1},

publisher = {MDPI},

abstract = {The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage; double-strand breaks—DSB; 8-hydroxy-2′-deoxyguanosine-8-OHdG; abasic site—AP sites), DNA damage response parameters, and global methylation in the model organism Acheta domesticus. Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms. © 2022 by the authors.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85131527033,

title = {Does age pay off? Effects of three-generational experiments of nanodiamond exposure and withdrawal in wild and longevity-selected model animals},

author = { M. Augustyniak and A. Babczyńska and M. Dziewięcka and B. Flasz and J. Karpeta-Kaczmarek and A. Kędziorski and B. Mazur and K. Rozpędek and R. Seyed Alian and M. Skowronek and E. Świerczek and A. Świętek and M. Tarnawska and Kl. Wiśniewska and P. Ziętara},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131527033&doi=10.1016%2fj.chemosphere.2022.135129&partnerID=40&md5=a9ef4d24a7c7b03b85af2b6ebf7bc9e4},

doi = {10.1016/j.chemosphere.2022.135129},

issn = {00456535},

year  = {2022},

date = {2022-01-01},

journal = {Chemosphere},

volume = {303},

publisher = {Elsevier Ltd},

abstract = {Nanodiamonds (NDs) are considered a material with low toxicity. However, no studies describe the effects of ND withdrawal after multigenerational exposure. The aim was to evaluate ND exposure (in the 1st and 2nd generations) effects at low concentrations (0.2 or 2 mg kg−1) and withdrawal (in the 3rd generation) in the wild (H) and longevity-selected (D) model insect Acheta domesticus. We measured selected oxidative stress parameters, immunity, types of cell death, and DNA damage. Most of the results obtained in the 1st generation, e.g., catalase (CAT), total antioxidant capacity (TAC), heat shock proteins (HSP70), defensins, or apoptosis level, confirmed no significant toxicity of low doses of NDs. Interestingly, strain-specific differences were observed. D-strain crickets reduced autophagy, the number of ROS+ cells, and DNA damage. The effect can be a symptom of mobilization of the organism and stimulation of physiological defense mechanisms in long-living organisms. The 2nd-generation D-strain insects fed ND-spiked food at higher concentrations manifested a reduction in CAT, TAC, early apoptosis, and DNA damage, together with an increase in HSP70 and defensins. ROS+ cells and cells with reduced membrane potential and autophagy did not differ significantly from the control. H-strain insects revealed a higher number of ROS+ cells and cells with reduced membrane potential, decreased CAT activity, and early apoptosis. Elimination of NDs from the diet in the 3rd generation did not cause full recovery of the measured parameters. We noticed an increase in the concentration of HSP70 and defensins (H-strain) and a decrease in apoptosis (D-strain). However, the most visible increase was a significant increase in DNA damage, especially in H-strain individuals. The results suggest prolonged adverse effects of NDs on cellular functions, reaching beyond “contact time” with these particles. Unintentional and/or uncontrolled ND pollution of the environment poses a new challenge for all organisms inhabiting it, particularly during multigenerational exposure. © 2022 The Authors},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85117927480,

title = {The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus)},

author = { J. Francikowski and M. Potrzebska and E. Szulińska and M. Tarnawska and Z. Rádai and B. Łozowski and B. Baran and M. Krzyżowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117927480&doi=10.1038%2fs41598-021-00393-5&partnerID=40&md5=1c5df52d5f93052a5bf849a3c33f4229},

doi = {10.1038/s41598-021-00393-5},

issn = {20452322},

year  = {2021},

date = {2021-01-01},

journal = {Scientific Reports},

volume = {11},

number = {1},

publisher = {Nature Research},

abstract = {Tryptophan (TRP) is one of the essential amino acids in the animal body. Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level. It has been shown to have a number of essential functions, such as in the production of other biologically active molecules. The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect—house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)—white eye. This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition. A parallel goal was to compare the response of both cricket strains to HMD. ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio. Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content. A decrease in food intake was also observed in the groups on HMD. However, no changes in imago body weight and water content were observed. The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain. At the same time, a stronger detrimental effect of fructose than of glucose was apparent. Sex was found to be a modulating factor in the response to HMD. © 2021, The Author(s).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85112577869,

title = {Multigenerational selection towards longevity changes the protective role of vitamin C against graphene oxide-induced oxidative stress in house crickets},

author = { B. Flasz and M. Dziewięcka and A. Kędziorski and M. Tarnawska and J. Augustyniak and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85112577869&doi=10.1016%2fj.envpol.2021.117996&partnerID=40&md5=7ef2db048ee15bffac23fcd838fd4ba7},

doi = {10.1016/j.envpol.2021.117996},

issn = {02697491},

year  = {2021},

date = {2021-01-01},

journal = {Environmental Pollution},

volume = {290},

publisher = {Elsevier Ltd},

abstract = {This research was designed to investigate changes that can arise in an invertebrate organism due to stress caused by a strong prooxidant, graphene oxide (GO), and a potent antioxidant, vitamin C. The study aimed to investigate if vitamin C may support convalescence after chronic GO intoxication. We investigated the toxicity of chronic dietary graphene oxide administration in house cricket (Acheta domesticus) types: wild and selected for longevity (with a better developed antioxidant system; conducive to long life). Vitamin C was applied immediately after cessation of graphene oxide intoxication to check if it can support the remedial effect. The condition of cells, DNA stability, catalase activity, and the reproduction potential, measured as the Vitellogenin (Vg) protein expression level, were investigated in control and GO treated groups, recovery groups (-GO), and recovery groups with Vit. C (-GO + Vit.C). In this study vitamin C had no evident remedial effect on the house crickets exposed to graphene oxide. Most probably, the mechanism of vitamin C action, in case of intoxication with nanoparticles, is much more complicated. In the context of the results obtained, it is worth considering whether Vit. C, applied after GO intoxication, causes further disturbance of homeostasis in terms of the cells' redox potential. © 2021 Elsevier Ltd},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85116966176,

title = {High fat, high sugar diet and djos bariatric surgery influence plasma levels of fetuin-b, growth differentiation factor-15, and pentraxin 3 in diet-induced obese sprague–dawley rats},

author = { J. Poloczek and M. Tarnawska and E. Chełmecka and P. Łaszczyca and J. Gumprecht and D. Stygar},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116966176&doi=10.3390%2fnu13103632&partnerID=40&md5=8f3785c8a936ad636f8864e971f4395f},

doi = {10.3390/nu13103632},

issn = {20726643},

year  = {2021},

date = {2021-01-01},

journal = {Nutrients},

volume = {13},

number = {10},

publisher = {MDPI},

abstract = {The liver plays a central role in glucose and fatty acid metabolism and acts as an endocrine organ that secretes hepatokines with diverse systemic effects. The study aimed to examine the influence of duodenojejunal omega switch (DJOS) bariatric surgery in combination with different diets on glucose administration parameters and hepatokines levels. After 8 weeks on high fat, high sugar diet (HFS) or control diets (CD), Sprague–Dawley rats underwent DJOS or SHAM (control) surgery. For the next 8 weeks after the surgery, half of DJOS and SHAM-operated animals were kept on the same diet as before, and half had a diet change. The oral glucose tolerance test (OGTT) was performed three times: 8 weeks before and 4 and 8 weeks after surgery. Fetuin-B, growth differentiation factor-15 (GDF-15), pentraxin 3 (PTX3) plasma levels were analyzed. DJOS surgery had a beneficial effect on oral glucose tolerance test (OGTT) results and the area under the curve (AUCOGTT). The OGTT results depended on the time elapsed after the surgery, the type of diet used, the surgery performed, and the interaction between these factors. DJOS bariatric surgery reduced fetuin-B and GDF15 plasma levels. Interaction between the type of surgery performed and diet used influenced the fetuin-B and PTX-3 plasma levels. A dietary regime is essential to achieve therapeutic and clinical goals after bariatric surgery. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85105467182,

title = {Multigenerational graphene oxide intoxication results in reproduction disorders at the molecular level of vitellogenin protein expression in Acheta domesticus},

author = { B. Flasz and M. Dziewięcka and A. Kędziorski and M. Tarnawska and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105467182&doi=10.1016%2fj.chemosphere.2021.130772&partnerID=40&md5=412a7ee17abcd00614fdde8751e5fd1d},

doi = {10.1016/j.chemosphere.2021.130772},

issn = {00456535},

year  = {2021},

date = {2021-01-01},

journal = {Chemosphere},

volume = {280},

publisher = {Elsevier Ltd},

abstract = {The anthropogenic activities may lead to accumulation of graphene oxide (GO) pollution in the environment. Organisms exposed to chronic or multigenerational GO intoxication can present reproduction depletion. Vitellogenin (Vg) has been used as a parameter for evaluating female fertility due to its importance in embryo nutrition. In this study, we used a promising model organism, Acheta domesticus, which was intoxicated with GO in food for three generations. The aim of the study was to investigate the process of Vg synthesis in crickets depending on the exposure time, GO concentration, and age of the females. The results revealed that chronic GO intoxication had adverse effects on the Vg expression pattern. The 1st generation of insects showing low Vg expression was most affected. The 2nd generation of A. domesticus presented a high Vg expression. The last investigated generation seemed to cope with stress caused by GO, and the Vg expression was balanced. We suggest that the epigenetic mechanisms may play a role in the information transfer to the next generations on how to react to the risk factor and keep reproduction at a high rate. We suspect that chronic GO intoxication can disturb the regular formation of the Vg quaternary structure, resulting in consequences for developing an embryo. © 2021 Elsevier Ltd},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85095429320,

title = {Transmission of Pantoea ananatis, the causal agent of leaf spot disease of maize (Zea mays), by western corn rootworm (Diabrotica virgifera virgifera LeConte)},

author = { K. Krawczyk and J. Foryś and M. Nakonieczny and M. Tarnawska and P.K. Bereś},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85095429320&doi=10.1016%2fj.cropro.2020.105431&partnerID=40&md5=dff84c6a39823dd2dcd518488a06b411},

doi = {10.1016/j.cropro.2020.105431},

issn = {02612194},

year  = {2021},

date = {2021-01-01},

journal = {Crop Protection},

volume = {141},

publisher = {Elsevier Ltd},

abstract = {The aim of this study was to determine the role of Diabrotica virgifera virgifera (western corn rootworm; WCR) as a potential insect vector of the pathogenic bacterium Pantoea ananatis. The experiment included a greenhouse test to investigate if WCR was able to transfer the pathogenic bacteria from infected to healthy maize plants. Adult WCR specimens collected from maize fields near Rzeszów were used in the experiment. The plant materials were sweetcorn plants of the Waza variety. Pure cultures of previously verified P. ananatis strain M241 were the source of the inoculum. Insects caught under natural conditions were incubated in an isolator containing pathogen-negative plants. Randomly selected insects were then examined for the presence of bacterial pathogens of maize in their digestive tract. Pathogen-negative insects were used in the next stage of the experiment still carried out in isolators, in which the insects foraged on maize seedlings previously infected with P. ananatis. The control group consisted of healthy, uninfected insects and plants. After the incubation period, the presence of bacterial pathogens in the gastrointestinal tract of the WCR specimens was confirmed. Subsequent insects that acquired P. ananatis were bred on pathogen-negative maize plants. After incubation, the presence of pathogenic bacteria in the body of the examined WCR beetles was confirmed, and the presence of symptoms of the bacterial disease was noted on maize plants, fulfilling Koch's postulates and indicating that the WCR is a vector of P. ananatis on maize plants. This is the first report on the vectorization of P. ananatis by D. virgifera globally. © 2020 Elsevier Ltd},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85105631786,

title = {Effects of cadmium on mitochondrial structure and function in different organs: studies on the soil centipede Lithobius forficatus (Myriapoda, Chilopoda)},

author = { M.M. Rost-Roszkowska and I. Poprawa and Ł. Chajec and A. Chachulska-Żymełka and G. Wilczek and P. Wilczek and M. Tarnawska and S. Student and M. Leśniewska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105631786&doi=10.1080%2f24750263.2021.1912199&partnerID=40&md5=521cb3648cd8129014a1058fea253b44},

doi = {10.1080/24750263.2021.1912199},

issn = {24750263},

year  = {2021},

date = {2021-01-01},

journal = {European Zoological Journal},

volume = {88},

number = {1},

pages = {632-648},

publisher = {Taylor and Francis Ltd.},

abstract = {Mitochondria are organelles that play a crucial role in cell physiology, cell death, and aging. They are among the first responders to different stressors that originate from the environment. Cadmium as a heavy metal affects different levels of body organization: from organs through tissues and cells to organelles. Based on our previous research results, we decided to check how the exposure to cadmium affects the functioning of mitochondria in different organs of soil living centipede Lithobius forficatus. The activity of mitochondria in somatic and germ cells has been analyzed using transmission electron microscopy (TEM), confocal microscopy, and flow cytometry. Changes in the mitochondrial membrane potential and mitochondrial dismutase (MnSOD) activity in relation to the accumulation of reactive oxygen species (ROS) caused by cadmium exposure have been studied. Individuals were divided into 3 experimental groups depending on cadmium concentration in soil. Changes in mitochondrial ultrastructure caused by cadmium are tissue-dependent and associated with an increase of ROS levels. The system of ROS and MnSOD activation works more efficiently in the case of gonads than in the digestive system. While the short-term cadmium exposure alters the fine structure of both the somatic and germ-line cells in gonads, the long-term cadmium exposure causes mitochondrial ultrastructure regeneration. © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85102487461,

title = {Stress proteins concentration in caged Cyprinus carpio as a tool to monitor ecological stability in a model dam reservoir},

author = { A. Babczyńska and M. Tarnawska and P. Łaszczyca and P. Migula and B. Łozowski and A. Woźnica and I. Irnazarow and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102487461&doi=10.24425%2faep.2021.136452&partnerID=40&md5=5aafdbafbb462de53a29be6faf4a67ef},

doi = {10.24425/aep.2021.136452},

issn = {20834772},

year  = {2021},

date = {2021-01-01},

journal = {Archives of Environmental Protection},

volume = {47},

number = {1},

pages = {101-111},

publisher = {Polska Akademia Nauk},

abstract = {Variability of stress proteins concentration in caged carp exposed to transplantation experiment model dam reservoir was caused only by natural (climatic and biological) conditions. Thus, the reference data of stress proteins concentration range in young carp individuals were obtained. Metallothionein, HSP70 and HSP90 protein concentrations as biomarkers were assayed in the livers, gills and muscles of six-month-old (summer) or nine-month-old (autumn) carp individuals in relation to the site of encaging, season (summer or autumn), the term of sampling (1; 2 or 3 weeks after the transplantation) and tissue. Physicochemical analyses of the condition of water as well as pollution detection were conducted during each stage of the experiment. As the result of this study, the range of the variability of the stress protein concentration in young carp individuals was obtained. According to the analyses of the aquatic conditions of a reservoir with no detectable pollutants, we conclude that the variability in the stress protein concentration levels in the groups that were compared is solely the result of the natural conditions. Future regular monitoring of the reservoir using the transplantation method and young carp individuals will be both possible and reliable. Moreover, the range of variability in the stress protein concentrations that were measured in the young C. carpio individuals acquired from the model dam reservoir in relation to all of the studied factors may be applied in the monitoring of any other similar reservoir. © 2021. The Author(s).},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85091907971,

title = {Energy reserves, oxidative stress and development traits of Spodoptera exigua Hübner individuals from cadmium strain},

author = { A. Kafel and A. Babczyńska and A. Zawisza-Raszka and M. Tarnawska and A. Płachetka-Bożek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091907971&doi=10.1016%2fj.envpol.2020.115366&partnerID=40&md5=db2b849cc1b11998281831996100c168},

doi = {10.1016/j.envpol.2020.115366},

issn = {02697491},

year  = {2021},

date = {2021-01-01},

journal = {Environmental Pollution},

volume = {268},

publisher = {Elsevier Ltd},

abstract = {Cadmium as a common environmental stressor may exert highly toxic effects on herbivorous insects. The question was whether possible elevation of an oxidative stress and imbalance of energetic reserves in insects may depend on developmental stage, sex and insect population's multigenerational history of exposure to cadmium. So, the aim of this study was to compare of the development traits, total antioxidant capacity, lipid peroxidation, RSSR to RSH ratio and the concentration of carbohydrates, glycogen, lipids and proteins in whole individuals (larvae or pupae) of Spodoptera exigua originating from two strains: control and selected over 120 generations with sublethal metal concentration (44 Cd mg per dry weight of diet). Generally, the increase of the protein, carbohydrates, glycogen concentration and lipid peroxidation decrease with age of the larvae were found. Revealed cases of a higher mobilisation of carbohydrates and proteins, and changes in total antioxidant capacity or lipid peroxidation, in individuals being under metal exposure, occurred in strain-depended mode. Short-term Cd exposure effect was connected with possible higher engagement of proteins and glycogen in detoxification processes, but also higher concentration of lipid peroxidation. In turn, for long-term Cd exposure effect lower lipids concentration and higher thiols usage seemed to be more specific. © 2020The study shown differences in defence strategies, energetic substances cumulation with development stages between insect pest representatives from strains differed in tolerance to cadmium. © 2020},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85075341430,

title = {Esterase activity and heat shock protein levels in western corn rootworm beetles (Diabrotica virgifera virgifera LeConte, Coleoptera: Chrysomelidae) after pesticide exposure},

author = { M. Tarnawska and P.K. Bereś and S. Drzewiecki and J. Guzik and P. Migula and K.R. Brom and K. Brzozowska-Wojoczek and B. Doleżych and M. Nakonieczny},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075341430&doi=10.1080%2f09670874.2019.1687958&partnerID=40&md5=835701f0af70ec5cf8404c94b54dccec},

doi = {10.1080/09670874.2019.1687958},

issn = {09670874},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Pest Management},

volume = {67},

number = {2},

pages = {99-107},

publisher = {Taylor and Francis Ltd.},

abstract = {The aim of this study was to investigate how the exposure of the western corn rootworm beetle (WCR) to dimethoate might affect their ability to detoxify and Hsps response. The activity of the esterases: acetylcholinesterase (AChE) and carboxylesterase (CbE) and the level of Hsp90, Hsp70 and Hsp40 was assayed in adult individuals 1 h and 24 h after a topical pesticide application. A slight increase in the esterase activity 1 h after pesticide exposure was followed by a significant decrease in AChE after 24 h. The ELISA method revealed differences in the Hsps concentrations that were dependent on the protein family. Western Blotting data confirmed these results. The esterase activity and Hsps response are discussed. © 2019 Informa UK Limited, trading as Taylor & Francis Group.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85097069721,

title = {Autophagy: a necessary defense against extreme cadmium intoxication in a multigenerational 2D experiment},

author = { A. Babczyńska and A. Nowak and A. Kafel and B. Łozowski and M.M. Rost-Roszkowska and M. Tarnawska and M. Augustyniak and M.K. Sawadro and A.E. Molenda},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097069721&doi=10.1038%2fs41598-020-78316-z&partnerID=40&md5=7ec842d0f44a16b6784907a0728b2823},

doi = {10.1038/s41598-020-78316-z},

issn = {20452322},

year  = {2020},

date = {2020-01-01},

journal = {Scientific Reports},

volume = {10},

number = {1},

publisher = {Nature Research},

abstract = {Autophagy is a natural process that aims to eliminate malfunctioning cell parts, organelles or molecules under physiological conditions. It is also induced in response to infection, starvation or oxidative stress to provide energy in case of an energy deficit. The aim of this 2-dimensional study was to test if, and if so, how, this process depends on the concentration of cadmium in food (with Cd concentrations from 0 to 352 μg of Cd per g of food (dry weight)—D1 dimension) and the history of selection pressure (160 vs 20 generations of exposure to Cd—D2 dimension). For the study, the 5th instar larvae of a unique strain of the moth Spodoptera exigua that was selected for cadmium tolerance for 160 generations (44 μg of Cd per g of food (dry weight)), as well as 20-generation (11; 22 and 44 μg of Cd per g of food (dry weight)) and control strains, were used. Autophagy intensity was measured by means of flow cytometry and compared with life history parameters: survivability and duration of the 3rd larval stage. The highest values of autophagy markers were found in the groups exposed to the highest Cd concentration and corresponded (with a significant correlation coefficient) to an increased development duration or decreased survivorship in the respective groups. In conclusion, autophagy is probably initiated only if any other defense mechanisms, e.g., antioxidative mechanisms, are not efficient. Moreover, in individuals from pre-exposed populations, the intensity of autophagy is lower. © 2020, The Author(s).},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85088645994,

title = {DNA damage in Spodoptera exigua after multigenerational cadmium exposure - A trade-off between genome stability and adaptation},

author = { M. Augustyniak and M. Tarnawska and M. Dziewięcka and A. Kafel and M.M. Rost-Roszkowska and A. Babczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088645994&doi=10.1016%2fj.scitotenv.2020.141048&partnerID=40&md5=8e46a0514984c19ad9d2fba14b49e9bf},

doi = {10.1016/j.scitotenv.2020.141048},

issn = {00489697},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {745},

publisher = {Elsevier B.V.},

abstract = {Human activity is a serious cause of extensive changes in the environment and a constant reason for the emergence of new stress factors. Thus, to survive and reproduce, organisms must constantly implement a program of adaptation to continuously changing conditions. The research presented here is focused on tracking slow changes occurring in Spodoptera exigua (Lepidoptera: Noctuidae) caused by multigenerational exposure to sub-lethal cadmium doses. The insects received food containing cadmium at concentrations of 5, 11, 22 and 44 μg per g of dry mass of food. The level of DNA stability was monitored by a comet assay in subsequent generations up to the 36th generation. In the first three generations, the level of DNA damage was high, especially in the groups receiving higher doses of cadmium in the diet. In the fourth generation, a significant reduction in the level of DNA damage was observed, which could indicate that the desired stability of the genome was achieved. Surprisingly, however, in subsequent generations, an alternating increase and decrease was found in DNA stability. The observed cycles of changing DNA stability were longer lasting in insects consuming food with a lower Cd content. Thus, a transient reduction in genome stability can be perceived as an opportunity to increase the number of genotypes that undergo selection. This phenomenon occurs faster if the severity of the stress factor is high but is low enough to allow the population to survive. © 2020 Elsevier B.V.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85086727685,

title = {Vitellogenin expression, DNA damage, health status of cells and catalase activity in Acheta domesticus selected according to their longevity after graphene oxide treatment},

author = { B. Flasz and M. Dziewięcka and A. Kędziorski and M. Tarnawska and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086727685&doi=10.1016%2fj.scitotenv.2020.140274&partnerID=40&md5=0dbe9ad21f1a92fd747f33cc48150904},

doi = {10.1016/j.scitotenv.2020.140274},

issn = {00489697},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {737},

publisher = {Elsevier B.V.},

abstract = {The increased use of graphene oxide (GO) raises worrisome questions regarding its possible threat to various ecosystems. Invertebrates represent valuable organisms for environmental studies. The lifespan can influence the ability to cope with toxins, especially those that act via oxidative stress. Two strains of Acheta domesticus, which are selected for longevity, were tested. The main aim was to investigate how GO, when administrated in food, affects: the condition of cells, DNA stability, ROS generation and the reproduction potential (the Vitellogenin (Vg) protein expression). The “recovery effect” – after removing GO from the diet for 15 days – was also measured. The results revealed different responses to GO in the wild (H) and long-living (D) strains. The D strain had a higher catalase activity compared to the H strain on the 25th day of the imago stage. Removing GO from the food resulted in a decrease in the catalase activity to the level of the control. On the 5th day of the imago stage, the H strain had a higher cell mortality than the D strain in the GO-intoxicated groups. There was more DNA damage in the H strain compared to the long-living strain. A remedial effect was seen after the GO was removed from the diet. The total Vg protein expression was higher in the H strain and lower in the D strain. The results indicated a GO concentration-dependent outcome. In both strains, removing the GO from the food led to a high Vg expression. The Vg expression after GO treatment, particularly translation and post-translational processing, should be studied in detail in the future. The D strain of crickets had more specialized mechanisms for maintaining homeostasis than the H strain. Organisms can fight off negative effects of GO, especially when they have systems that are well developed against oxidative stress. © 2020 Elsevier B.V.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85068259796,

title = {Protective role of zinc in Spodoptera exigua larvae under 135-generational cadmium exposure},

author = { M. Tarnawska and A. Babczyńska and K. Hassa and A. Kafel and A. Płachetka-Bożek and J. Augustyniak and M. Dziewięcka and B. Flasz and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068259796&doi=10.1016%2fj.chemosphere.2019.06.209&partnerID=40&md5=f08e57e4cdd129a976f96151aaf01fb5},

doi = {10.1016/j.chemosphere.2019.06.209},

issn = {00456535},

year  = {2019},

date = {2019-01-01},

journal = {Chemosphere},

volume = {235},

pages = {785-793},

publisher = {Elsevier Ltd},

abstract = {The aim of this study was to investigate whether zinc supplementation modulates cadmium toxicity in the beet armyworm Spodoptera exigua selected for 135 generations towards cadmium tolerance. To achieve this, larvae originating from three laboratory populations of S. exigua (control strain - C; cadmium-intoxicated for 135 generations strain - Cd; and control strain intoxicated with Cd for 1 generation - CCd) were additionally exposed to zinc in three concentrations (Zn1; 400 μg Zn·g−1 dry mass of food; Zn2; 200 μg Zn·g−1 dry mass of food; Zn3; 100 μg Zn·g−1 dry mass of food). As the markers of toxicity, a life history traits (the duration of L4 and L5 stages), cellular (DNA damage indices) and biochemical parameters (ADP/ATP ratio and ATP and HSP70 concentrations) were chosen. The duration of larval stages of Zn supplemented larvae was prolonged, while cellular and biochemical indicators, in general, appeared to be lower in comparison to the insects from respective reference groups in each laboratory populations. Moreover, the range of the differences depended on zinc concentration in food. We can suspect that zinc supplementation contributed to the protection of S. exigua individuals against negative effects of cadmium intoxication, probably at the cost of growth rate. Significant differences in the response pattern between insects from different laboratory populations indicate that the influence of additional stress factors is dependent on the overall condition of animals and their previous adaptation to other stressors. © 2019 Elsevier Ltd},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85064267438,

title = {Microevolution or wide tolerance? Level of stress proteins in the beet armyworm Spodoptera eqigua hübner (Lepidoptera: Noctuidae) exposed to cadmium for over 150 generations},

author = { M. Tarnawska and A. Kafel and M. Augustyniak and M.M. Rost-Roszkowska and A. Babczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064267438&doi=10.1016%2fj.ecoenv.2019.04.017&partnerID=40&md5=5f3adb3246bf4dcbd5777ca3f52fe722},

doi = {10.1016/j.ecoenv.2019.04.017},

issn = {01476513},

year  = {2019},

date = {2019-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {178},

pages = {1-8},

publisher = {Academic Press},

abstract = {The aim of this study was to investigate whether the cadmium tolerance developed in the beet armyworm Spodoptera exigua selected for over 150 generations may be related to synthesis of the stress proteins metallothioneins (Mts) and 70 kDa heat shock proteins (HSP70). To achieve this, six S. exigua strains (control; k), 150-generation Cd exposure strain (cd), and four 18-generation Cd exposure strains differing in Cd concentration (cd44; cd22; cd11; cd5) were reared. Stress protein level was measured in the midgut of the 5th larval stage after 1–6, 12 and 18 generations. Cd contents was measured in the pupae. Unlike Cd concentration, which depended on metal contents in food but was not generation-dependent, the pattern of Mts and HSP70 concentrations changed in experimental strains from generation to generation. Stress protein levels in the insects exposed to the highest Cd concentration (the same as in the 150-generation Cd exposure strain), initially higher than in the control strain, after the 12th generation did not differ from the level measured in the control strains. It seems therefore that stress proteins play a protective role in insects of lower tolerance to cadmium. The tolerance developed during multigenerational exposure probably relies on mechanisms other than Mt and HSP70 synthesis. © 2019 Elsevier Inc.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85062391000,

title = {Immune response of juvenile common carp (Cyprinus carpio L.) exposed to a mixture of sewage chemicals},

author = { M. Tarnawska and M. Augustyniak and P. Łaszczyca and P. Migula and I. Irnazarow and M. Krzyżowski and A. Babczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062391000&doi=10.1016%2fj.fsi.2019.02.049&partnerID=40&md5=d42712874f989fa17f5c77ff077e7bc5},

doi = {10.1016/j.fsi.2019.02.049},

issn = {10504648},

year  = {2019},

date = {2019-01-01},

journal = {Fish and Shellfish Immunology},

volume = {88},

pages = {17-27},

publisher = {Academic Press},

abstract = {Pharmaceuticals and household chemicals are important components of municipal sewage. Many of them are biologically active, disrupting not only hormonal regulation of aquatic animals but also, indirectly, disturbing their immunological protection. In the environment, chemicals rarely act as individual substances, but as elements of mixtures. Therefore, the aim of this study was to check whether the acute laboratory exposure of common carp juveniles to a mixture of ibuprofen, sodium dodecyl sulphate (SDS), dimethyl sulfoxide (DMSO) and 17 α-ethynylestradiol in increasing concentrations, modifies the levels of innate immunity (lysozyme; C-reactive protein) as well as general stress (metallothioneins; heat shock proteins HSP70) markers in brain, liver, gills, spleen and mucus. The levels of the markers were measured by an immunodetection technique. Not only do the pharmaceuticals and household chemicals impair immunological reactions of young carp in various tissues but also do that in a concentration-dependent manner in the liver, gills, spleen and mucus. This has a very important implication, since it may result in higher sensitivity of young fish to pathogens due to energy allocation to defence processes. The comparisons of the pattern of stress reactions in the studied organ samples indicated that mucus appeared to be a good, non-invasive material for monitoring of environmental state and fish conditions. © 2019},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85074111126,

title = {Relationship between ROS production, MnSOD activation and periods of fasting and re-feeding in freshwater shrimp Neocaridina davidi (Crustacea, Malacostraca)},

author = { A. Włodarczyk and G. Wilczek and P. Wilczek and S. Student and A. Ostróżka and M. Tarnawska and M.M. Rost-Roszkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074111126&doi=10.7717%2fpeerj.7399&partnerID=40&md5=60bccc587e4acdd09cf38ccdc212914d},

doi = {10.7717/peerj.7399},

issn = {21678359},

year  = {2019},

date = {2019-01-01},

journal = {PeerJ},

volume = {2019},

number = {9},

publisher = {PeerJ Inc.},

abstract = {The middle region of the digestive system, the midgut of freshwater shrimp Neocaridina davidi is composed of a tube-shaped intestine and the hepatopancreas formed by numerous caeca. Two types of cells have been distinguished in the intestine, the digestive cells (D-cells) and regenerative cells (R-cells). The hepatopancreatic tubules have three distinct zones distinguished along the length of each tubule-the distal zone with R-cells, the medial zone with differentiating cells, and the proximal zone with F-cells (fibrillar cells) and B-cells (storage cells). Fasting causes activation of cell death, a reduction in the amount of reserve material, and changes in the mitochondrial membrane potential. However, here we present how the concentration of ROS changes according to different periods of fasting and whether re-feeding causes their decrease. In addition, the activation/deactivation of mitochondrial superoxide dismutase (MnSOD) was analyzed. The freshwater shrimps Neocaridina davidi (Crustacea; Malacostraca; Decapoda) were divided into experimental groups: animals starved for 14 days, animals re-fed for 4, 7, and 14 days. The material was examined using the confocal microscope and the flow cytometry. Our studies have shown that long-term starvation increases the concentration of free radicals and MnSOD concentration in the intestine and hepatopancreas, while return to feeding causes their decrease in both organs examined. Therefore, we concluded that a distinct relationship between MnSOD concentration, ROS activation, cell death activation and changes in the mitochondrial membrane potential occurred. Copyright © 2019 Włodarczyk et al.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85031894354,

title = {Cross tolerance in beet armyworm: long-term selection by cadmium broadens tolerance to other stressors},

author = { M. Augustyniak and M. Tarnawska and A. Babczyńska and A. Kafel and A. Zawisza-Raszka and B. Adamek and A. Płachetka-Bożek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031894354&doi=10.1007%2fs10646-017-1865-5&partnerID=40&md5=17ff4c7a2acb645b6758e1bf3f98a15d},

doi = {10.1007/s10646-017-1865-5},

issn = {09639292},

year  = {2017},

date = {2017-01-01},

journal = {Ecotoxicology},

volume = {26},

number = {10},

pages = {1408-1418},

publisher = {Springer New York LLC},

abstract = {Long lasting exposure of animals to stressing factor may lead to the selection of population able to cope with the stressor at lower cost than unexposed individuals. The aim of this study was to assess whether 130-generational selection of a beet armyworm to cadmium in food might have induced tolerance also to other stressors. The potential tolerance was assessed by means of unspecific stress markers: HSP70 concentration, DNA damage level, and energy budget indices in L5 larval instars of beet armyworm. The animals originated from Cd-exposed and control strains exposed additionally in a short-term experiment to high/low temperature or pesticide—spinosad. The application of the additional stressors caused, in general, an increase in the levels of studied parameters, in a strain-dependent manner. The most significant increase was found in HSP70 level in the individuals from the Cd-strain exposed to various spinosad concentration. Therefore, multigenerational contact with cadmium caused several changes that enable the insect to survive under a chronic stress, preparing the organism to the contact with an additional, new stressor. This relationship may be described as a sort of cross tolerance. This may, possibly, increase the probability of population survivorship and, at the same time, decrease the efficiency of pesticide-based plant protection efforts. © 2017, The Author(s).},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84999759002,

title = {Phenotypic plasticity, epigenetic or genetic modifications in relation to the duration of Cd-exposure within a microevolution time range in the beet armyworm},

author = { M. Augustyniak and A. Płachetka-Bożek and A. Kafel and A. Babczyńska and M. Tarnawska and A. Janiak and A. Loba and M. Dziewięcka and J. Karpeta-Kaczmarek and A. Zawisza-Raszka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84999759002&doi=10.1371%2fjournal.pone.0167371&partnerID=40&md5=71387ac76e18d04e4111760b53546841},

doi = {10.1371/journal.pone.0167371},

issn = {19326203},

year  = {2016},

date = {2016-01-01},

journal = {PLoS ONE},

volume = {11},

number = {12},

publisher = {Public Library of Science},

abstract = {In the case of the pests inhabiting metal polluted or fields where the use of pesticides is common, a natural selection of resistant individuals can occur. This may pose serious problems for humans, agriculture, as well as the economies of many countries. In this study, the hypothesis that multigenerational (120 generations) exposure to cadmium of a beet armyworm population could be a selecting factor toward a more efficient DNA protection was verified. The hemocytes of individuals from two culture strains (control and Cd-exposed) were treated with H2O2 (a DNA-damaging agent) or PBS (reference). The level of DNA damage was assessed using the Comet assay immediately and 5, 15 and 30 min. after the treatment. The immediate result of the contact with H2O2 was that the level of DNA damage in the hemocytes of the insects from both strains increased significantly. However, in the cells of the Cd-exposed individuals, the level of DNA damage decreased over time, while in the cells from the control insects it remained at the same level with no evidence of repair. These results suggest that efficient defense mechanisms may exist in the cells of insects that have prolonged contact with cadmium. Some evolutionary and trade-off aspects of the phenomenon are discussed. In a wider context, comparing the results obtained in the laboratory with field studies may be beneficial for understanding basic mechanisms of the resistance of an organism. To summarize, the high potential for the repair of DNA damage that was observed in the insects from the cadmium strain may confirm the hypothesis that multigenerational exposure to that metal may possibly contribute to the selection of insects that have a wider tolerance to oxidative stress. However, our investigations of polymorphism using AFLP did not reveal differences between the two main insect strains. © 2016 Augustyniak et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {23},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84954403049,

title = {Population dynamics of Western corn rootworm beetles on different varieties of maize identified using pheromone and floral baited traps},

author = { P.K. Bereś and S. Drzewiecki and M. Nakonieczny and M. Tarnawska and J. Guzik and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84954403049&doi=10.1017%2fS0021859615000222&partnerID=40&md5=f84ebbbde7d3a78c9552c49f63a88a5c},

doi = {10.1017/S0021859615000222},

issn = {00218596},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Agricultural Science},

volume = {153},

number = {8},

pages = {1479-1490},

publisher = {Cambridge University Press},

abstract = {The present paper presents details of the population dynamics of Diabrotica v. virgifera LeConte beetles on six maize varieties of three different maturity classes, i.e. early (FAO 190), medium-early (FAO 250) and medium-late (FAO 280-300), grown in Central and Eastern Europe. The study was conducted in 2009/10 in the south-eastern part of Poland. The D. virgifera beetles were captured using two types of trap: pheromone (PTs) and floral-baited (FTs). Significant differences in beetle counts were found between: (i) the first and second year of the study (higher in 2009 than in 2010); (ii) the varieties of maize and their different growth stages; (iii) early and medium-late varieties of maize. Initially, depending on the availability of maize silk and pollen, the most numerous D. virgifera beetles were found on the early varieties followed by the medium-late varieties. The study also revealed a significant difference in the performance of the two types of trap used to monitor the number of adults, particularly during the initial period when arrival rates were high. Pheromone traps were more effective in catching D. virgifera beetles, especially during the flowering stage of maize. Floral-baited traps were most effective at the end of the active growth of maize, when the preferred food of D. virgifera was unavailable. © Cambridge University Press 2015.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84923569075,

title = {DNA damage in grasshopper Chorthippus brunneus (Orthoptera) hatchlings following paraquat exposure},

author = { M. Augustyniak and T. Nocon and A. Kędziorski and P. Łaszczyca and T. Sawczyn and M. Tarnawska and A. Zawisza-Raszka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923569075&doi=10.1016%2fj.chemosphere.2014.12.069&partnerID=40&md5=f1d2caec2debb3c806d9441a50130558},

doi = {10.1016/j.chemosphere.2014.12.069},

issn = {00456535},

year  = {2015},

date = {2015-01-01},

journal = {Chemosphere},

volume = {125},

pages = {212-219},

publisher = {Elsevier Ltd},

abstract = {Comet assay was applied to study genotoxic damage induced by paraquat (PQ) in brain cells of Chorthippus brunneus (Insecta: Orthoptera) hatchlings. Percentage of the comet fluorescence in the tail (TDNA), length of the comet tail (TL) and Olive tail moment (OTM) were used for quantitative assessment of the DNA damage. Multiple regression analysis supplemented standard statistical elaboration of the results. Increasing PQ concentrations applied either directly to the brain cells suspension (10; 50; and 250. μM PQ final concentration - in vitro protocol) or indirectly (50; 250; and 1250. μM PQ final concentration - in vivo protocol) provoked significant increase of oxidative damage to DNA (higher median TDNA and OTM values). The damage increased with time of exposure (0; 5; 15; and 30. min) following in vitro application, but decreased in longer interval (3 vs 24. h) after in vivo administration of paraquat. On contrary, median TL values did not correlate with paraquat concentration irrespectively of the exposure protocol. Possible reason of this discrepancy in light of paraquat toxicity is discussed. © 2015 Elsevier Ltd.},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85009892699,

title = {Glycolytic enzymes activity in the midgut of Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) adult and their seasonal changes},

author = { J. Guzik and M. Nakonieczny and M. Tarnawska and P.K. Bereś and S. Drzewiecki and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009892699&doi=10.1093%2fjisesa%2fiev036&partnerID=40&md5=c108792bd08349e23d6ec34a5bdce674},

doi = {10.1093/jisesa/iev036},

issn = {15362442},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Insect Science},

volume = {15},

number = {1},

publisher = {Library of the University of Arizona},

abstract = {The western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) is an important pest of maize. The diet of the D. virgifera imago is rich in starch and other polysaccharides present in cereals such as maize. Therefore, knowledge about enzymes involved in digestion of such specific food of this pest seems to be important. The paper shows, for the first time, the activities of main glycolytic enzymes in the midgut of D. virgifera imago: endoglycosidases (α-amylase; cellulase; chitinase; licheninase; laminarinase); exoglycosidases (α- and β-glucosidases; α- and β-galactosidases) and disaccharidases (maltase; isomaltase; sucrase; trehalase; lactase; and cellobiase). Activities of α-amylase, α-glucosidase, and maltase were the highest among assayed endoglycosidases, exoglycosidases, and disaccharidases, respectively. This indicates that in the midgut of D. virgifera imago a-amylase, α-glucosidase and maltase are important enzymes in starch hydrolysis and products of its digestion. These results lead to conclusion that inhibition of most active glycolytic enzymes of D. virgifera imago may be another promising method for chemical control of this pest of maize. © The Author 2015. Published by Oxford University Press on behalf of the Entomological Society of America.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84956691211,

title = {Expression of the Hsp40, Hsp70 and Hsp90 proteins in Colorado potato beetle (Leptinotarsa decemlineata Say) after the dimethoate treatment},

author = { K.R. Brom and B. Doleżych and M. Tarnawska and K. Brzozowska and M. Nakonieczny},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956691211&partnerID=40&md5=b0bec8c31a4ea850a312ef254dc2de33},

issn = {13020250},

year  = {2015},

date = {2015-01-01},

journal = {Journal of the Entomological Research Society},

volume = {17},

number = {2},

pages = {39-49},

publisher = {Gazi Entomological Research Society},

abstract = {Hsp40, Hsp70 and Hsp90 proteins exhibit increased level of expression in animals exposed to negative environmental conditions, including pesticides, but the measured effect depends on the class which particular Hsp is belonging to. In this study we examined Hsp level in the Colorado potato beetle exposed to pesticide dimethoate by using Western blotting and ELISA method. The highest level of expression was exhibited by Hsp90, while Hsp70 showed the lowest level of expression. There were no statistically significant differences in the expressions of Hsp40 and Hsp70 after the pesticide treatment. The highest concentration of Hsp90 was found in beetles 24 hours after the exposition to dimethoate, while the lowest was observed in insects 1 hour after the treatment. Hsps play a role in response to organophosphorous pesticide in Leptinotarsa decemlineata (Say; 1824), but there is not any simple correlation between Hsp levels and time of exposition. Hsp90 seems to be the most suitable protein to examine pesticide-provoked stress.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84924420592,

title = {Elemental distribution in reproductive and neural organs of the epilachna nylanderi (Coleoptera: Coccinellidae), a phytophage of nickel hyperaccumulator Berkheya coddii (Asterales: Asteraceae) by micro-PIXE},

author = { J. Mesjasz-Przybyłowicz and E. Orłowska and M. Augustyniak and M. Nakonieczny and M. Tarnawska and W.J. Przybyłowicz and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924420592&doi=10.1093%2fjisesa%2fieu014&partnerID=40&md5=b9fa04f75525193486d5820a32e309b4},

doi = {10.1093/jisesa/ieu014},

issn = {15362442},

year  = {2014},

date = {2014-01-01},

journal = {Journal of Insect Science},

volume = {14},

publisher = {Library of the University of Arizona},

abstract = {The phenomenon of metal hyperaccumulation by plants is often explained by a pathogen or herbivore defense hypothesis. However, some insects feeding on metal hyperaccumulating plants are adapted to the high level of metals in plant tissues. Former studies on species that feed on the leaves of Berkheya coddii Roessler 1958 (Asteraceae), a nickel-hyperaccumulating plant, demonstrated several protective mechanisms involved in internal distribution, immobilization, and elimination of Ni from the midgut and Malpighian tubules. These species are mainly coleopterans, including the lady beetle, Epilachna nylanderi (Mulsant 1850) (Coleoptera: Coccinellidae), collected from the ultramafic ecosystem near Barberton in South Africa. By performing particle-induced X-ray emission microanalysis elemental microanalysis (PIXE), this study examined whether Ni may be harmful to internal body systems that decide on insect reactivity (central nervous system [CNS]), their reproduction, and the relationships between Ni and other micronutrients. Data on elemental distribution of nine selected elements in target organs of E. nylanderi were compared with the existing data for other insect species adapted to the excess of metals. Micro-PIXE maps of seven regions of the CNS showed Ni mainly in the neural connectives, while cerebral ganglia were better protected. Concentrations of other bivalent metals were lower than those of Ni. Testis, compared with other reproductive organs, showed low amounts of Ni. Zn was effectively regulated at physiological dietary levels. In insects exposed to excess dietary Zn, it was also accumulated in the reproductive organs. Comparison of E. nylanderii with other insects that ingest hyperaccumulating plants, especially chrysomelid Chrysolina clathrata (Clark) (Coleoptera: Chrysomelidae), showed lower protection of the CNS and reproductive organs. © The Author 2014. Published by Oxford University Press on behalf of the Entomological Society of America.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79955156773,

title = {Micro-PIXE studies of Ni-elimination strategies in representatives of two families of beetles feeding on Ni-hyperaccumulating plant Berkheya coddii},

author = { P. Migula and W.J. Przybyłowicz and M. Nakonieczny and M. Augustyniak and M. Tarnawska and J. Mesjasz-Przybyłowicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955156773&doi=10.1002%2fxrs.1310&partnerID=40&md5=61672de73a5cbfaa8e978a3de14d6f8f},

doi = {10.1002/xrs.1310},

issn = {00498246},

year  = {2011},

date = {2011-01-01},

journal = {X-Ray Spectrometry},

volume = {40},

number = {3},

pages = {194-197},

abstract = {Plants may use the ability to hyperaccumulate toxic metals in their defence against herbivores. Insects can counteract and feed on toxic plants. Among grazers of Ni-hyperaccumulating Berkheya coddii, some beetles tolerate Ni (Chrysolina clathrata; former name Chrysolina pardalina; Epilachna nylanderi). The adaptive mechanisms against Ni toxicity were studied in these species using micro-PIXE quantitative elemental mapping and by microscopy techniques. Distinct differences were found in mechanisms used for Ni-elimination in both species. Functional comparisons and quantification of data extracted from selected micro-areas in target organs responsible for metal circulation (digestive tract and Malpighian tubules) were made. Similar detoxification mechanism is used against metals through spheric granules, which in E. nylanderi are formed mainly in the midgut, while in C. clathrata in Malpighian tubules and midgut. Elemental maps showed positive correlations of Ni and Zn in Malpighian tubules of E. nylanderi without such effect in C. clathrata. Quantitative relations of K+ and Cl- indicate higher osmotic pressure increasing reabsorption of water necessary for Ni transportation to the intestine lumen and further rejection outside the body. © 2011 John Wiley & Sons, Ltd.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-67549092938,

title = {Hsp70 level in progeny of aging grasshoppers from variously polluted habitats and additionally exposed to zinc during diapause},

author = { M. Augustyniak and M. Tarnawska and A. Babczyńska and M.G. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-67549092938&doi=10.1016%2fj.jinsphys.2009.04.009&partnerID=40&md5=c9468174a580b05f85fecf42df645691},

doi = {10.1016/j.jinsphys.2009.04.009},

issn = {00221910},

year  = {2009},

date = {2009-01-01},

journal = {Journal of Insect Physiology},

volume = {55},

number = {8},

pages = {735-741},

abstract = {The hsp70 level in the bodies of 1st instars of grasshoppers Chorthippus brunneus from unpolluted (Pilica) and polluted (Olkusz; Szopienice) sites and additionally exposed to various doses of zinc during diapause and embryonic development prior to hatching were measured by Western blotting. The main aim of our work was to assess the relationship between the age of female grasshoppers originating from variously polluted habitat and the hsp70 level in their progeny. Possible reasons for population variation in hsp70 levels were discussed. The hsp70 level in the offspring's body depended on the place of origin. The strongest expression of hsp70 was found in the bodies of larvae hatching from the eggs laid by young females from Pilica (reference site). In contrast, a low initial level of hsp70 in larvae from polluted sites, especially in young females' progeny, was observed. The application of zinc during diapause influenced the hsp70 level in grasshopper larvae; however, the direction of the changes depended on the insects' place of origin. In larvae from the reference site, and also (but to a lesser degree) from Olkusz, the increase in the hsp70 level after zinc treatment was most pronounced. Whereas in grasshoppers from Szopienice, zinc (in 100 μg g-1 dry weight of sand) did not change the hsp70 level, or (in 500 μg g-1 dry weight of sand) caused a reduction in hsp70. The differences may result from maternal effects; however, possible adaptation also cannot be excluded. To confirm this statement further studies are needed. © 2009 Elsevier Ltd. All rights reserved.},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-35548947013,

title = {Short-term effects of dimethoate on metabolic responses in Chrysolina pardalina (Chrysomelidae) feeding on Berkheya coddii (Asteraceae), a hyper-accumulator of nickel},

author = { M. Augustyniak and P. Migula and J. Mesjasz-Przybyłowicz and M. Tarnawska and M. Nakonieczny and A. Babczyńska and W.J. Przybyłowicz and M.G. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-35548947013&doi=10.1016%2fj.envpol.2007.01.018&partnerID=40&md5=144d40e423946ba636a4893b7c289520},

doi = {10.1016/j.envpol.2007.01.018},

issn = {02697491},

year  = {2007},

date = {2007-01-01},

journal = {Environmental Pollution},

volume = {150},

number = {2},

pages = {218-224},

abstract = {Berkheya coddii Roessler (Asteraceae) is a hyper-accumulator of nickel, which can be used in phytomining and phytoremediation. Chrysolina pardalina Fabricius (Chrysomelidae) is a phytophagous leaf beetle, which may be useful in controlling population levels of B. coddii after it has been introduced into a new habitat. The aim of this study was to investigate the response of C. pardalina to topical application of dimethoate. Data recorded included the activity of acetylcholinesterase (AChE), the concentration of glutathione (GSH), and the activity of selected enzymes connected with GSH metabolism. Assays were carried out several times during the first 24 h after exposure to dimethoate. At the dosages used in this study, dimethoate was not as toxic as expected. AChE activity was significantly decreased 14 and 24 h after application. GST activity was significantly decreased 24 h after application. GSTPx activity was significantly decreased 2, 14 and 24 h after application. GR activity was significantly increased 4 h after application. GSH concentration was significantly increased 24 h after application. Long-term exposure to high levels of nickel may have caused adaptive changes in the enzymes that enable C. pardalina to deal with other stressors, including organophosphate pesticides. © 2007 Elsevier Ltd. All rights reserved.},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34249997029,

title = {Nickel toxicity in the hepatopancreas of an isopod Porcellio scaber (Oniscidea)},

author = { M. Tarnawska and P. Migula and W.J. Przybyłowicz and J. Mesjasz-Przybyłowicz and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34249997029&doi=10.1016%2fj.nimb.2007.02.082&partnerID=40&md5=04fc0ac3f07189d95963f1c89d2c4068},

doi = {10.1016/j.nimb.2007.02.082},

issn = {0168583X},

year  = {2007},

date = {2007-01-01},

journal = {Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms},

volume = {260},

number = {1},

pages = {213-217},

abstract = {This study is focused on recognizing how the functional role of hepatopancreas, the main metal storage organ in woodlice, is affected by the excess of nickel, a toxic element to soil invertebrates. Chronic Ni toxicity (24 weeks) was studied on four groups of woodlice kept on dry shredded maple leaves contaminated with Ni at average concentrations of 0.1 μg g-1 (control), 8.0 μg g-1 (Ni1), 75 μg g-1 (Ni2) and 270 μg g-1(Ni3) dry weight. Micro-PIXE mapping of elemental distribution in the hepatopancreas of the Porcellio scaber woodlice was used to study relations between nickel and other elements in individuals exposed to different metal concentrations in the diet. Data were processed using GeoPIXE II software. Transmission electron microscopy (TEM) was used to check the relations between ultrastructural changes in hepatopancreatic cells and nickel tissue burden. Elemental mapping showed a dose-related nickel bioaccumulation in the hepatopancreas at concentrations from 3 μg g-1 (uncontaminated control animals) to nearly 840 μg g-1 (Ni3). Generally, nickel was distributed uniformly in small aggregations. A combined evaluation of elemental maps and electronograms showed that aggregations of nickel in arbitrarily selected micro-areas in PIXE maps could be the granular structures observed in TEM electronograms. The mechanism of Ni sequestration in the hepatopancreas could be similar to this used for cadmium or lead. The sequences of ultrastructural changes, which follow the increased burdens of Ni in the hepatopancreatic cells, were: the increase of intracellular electron-dense granules, increase in the number of myelin-like structures, intensified mitochondrial swelling and appearance of concentrically arranged, rough endoplasmic reticulum. © 2007 Elsevier B.V. All rights reserved.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34249995164,

title = {Nickel toxicity in the hindgut of an isopod Porcellio scaber (Oniscidea)},

author = { M. Tarnawska and P. Migula and W.J. Przybyłowicz and J. Mesjasz-Przybyłowicz and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34249995164&doi=10.1016%2fj.nimb.2007.02.081&partnerID=40&md5=6a0ed8cee8ea54fa364eaa9dcf05b161},

doi = {10.1016/j.nimb.2007.02.081},

issn = {0168583X},

year  = {2007},

date = {2007-01-01},

journal = {Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms},

volume = {260},

number = {1},

pages = {222-226},

abstract = {Studies on toxic effects of nickel were conducted in a chronic experiment on four groups of woodlice Porcellio scaber kept for 24 weeks on dry maple leaves containing Ni 0.1 (control), 8 (Ni1), 75 (Ni2) and 270 (Ni3) μg g-1 dry weight. Micro-PIXE elemental mapping complemented by proton backscattering was used to study relations between Ni and other elements in the hindgut. Data processed with GeoPIXE II demonstrated the highest amount of Ni in the hindgut of (Ni2) woodlice followed by the highest levels of Ca, Mn and Fe. Inverse relationship was observed between hindgut concentration of Zn and the Ni levels in the diet. Transmission electron microscopy (TEM) was used for checking possible relations between the ultrastructure of the hindgut epithelial cells and nickel tissue burden. In animals from all the "nickel groups" a series of ultrastructural alterations (disturbances in apical and basal membrane labyrinth and presence of lysosome-like structures with electron-dense material in the cytoplasm) was identified when compared with control woodlice. Large mitochondria with distinctly visible electron-dense precipitations inside them were present in the cells of animals from groups (Ni1) and (Ni3). In addition, a disturbed internal mitochondrial membrane system was observed in the cells of the (Ni2) woodlice group. © 2007 Elsevier B.V. All rights reserved.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-15444375342,

title = {Uptake of cadmium, lead, nickel and zinc from soil and water solutions by the nickel hyperaccumulator berkheya coddii},

author = { J. Mesjasz-Przybyłowicz and M. Nakonieczny and P. Migula and M. Augustyniak and M. Tarnawska and W.U. Reimold and C. Koeberl and W.J. Przybyłowicz and E. Głowacka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-15444375342&partnerID=40&md5=dc4ede8c4a0607a1f92bba5e4b45faf0},

issn = {00015296},

year  = {2004},

date = {2004-01-01},

journal = {Acta Biologica Cracoviensia Series Botanica},

volume = {46},

pages = {75-85},

abstract = {Berkheya coddii Roessler (Asteraceae), an endemic herbaceous and perennial nickel-hyperaccumulating plant growing on Ni-enriched ultramafic soils in South Africa, is perceived as a promising species for phytoremediation and phytomining due to its large biomass production and high Ni content. Total concentrations of a number of elements in mature leaves, soil and related bedrock were obtained. The average Ni concentration in leaves was 18,000 μg·g -1 dry mass, whereas in soil and bedrock the total amount of Ni was 1,300 μg·g-1 and 1,500 μg·g-1, respectively. Exceptionally high average Ni concentrations (55;000 ± 15;000 μg·g-1; n = 6) were found in B. coddii leaves from Songimvelo Game Reserve, including the highest-ever reported concentration of Ni in leaves (76;100 μg·g-1 - maximum value in a single sample). Young plants grown in pots with ultramafic soil accumulated small quantities of Cd, Pb or Zn, but the concentrations of these elements increased after the addition of metal solutions to the soil. Excised shoots immersed in concentrated solutions of Cd, Ni, Pb or Zn accumulated large amounts of these metals in the leaves. © Polish Academy of Sciences, Cracow 2004.},

note = {74},

keywords = {},

pubstate = {published},

tppubtype = {article}

}