• dr Elżbieta Szulińska
Stanowisko: Adiunkt
Jednostka: Wydział Nauk Przyrodniczych
Adres: 40-007 Katowice, ul. Bankowa 9
Piętro: III
Numer pokoju: 323
Telefon: (32) 359 1260
E-mail: elzbieta.szulinska@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 6507256971
Publikacje z bazy Scopus
2023
Wilczek, G.; Rost-Roszkowska, M. M.; Homa, J.; Szulińska, E.; Student, S.; Chajec, Ł.; Wiśniewska, Kl.; Surmiak-Stalmach, K.
In: European Zoological Journal, vol. 90, no. 2, pp. 624-642, 2023, ISSN: 24750263.
@article{2-s2.0-85165967465,
title = {How cadmium and copper change the sensitivity of the hemocytes of Steatoda grossa spider on immunostimulation: qualitative and quantitative analysis},
author = { G. Wilczek and M.M. Rost-Roszkowska and J. Homa and E. Szulińska and S. Student and Ł. Chajec and Kl. Wiśniewska and K. Surmiak-Stalmach},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85165967465&doi=10.1080%2f24750263.2023.2237989&partnerID=40&md5=d78134cfb2a4a0526661036b6548776b},
doi = {10.1080/24750263.2023.2237989},
issn = {24750263},
year = {2023},
date = {2023-01-01},
journal = {European Zoological Journal},
volume = {90},
number = {2},
pages = {624-642},
publisher = {Taylor and Francis Ltd.},
abstract = {The purpose of this study was to evaluate the sensitivity of hemocytes of adult Steatoda grossa females to cadmium and copper administered orally for 4 weeks or 12 months, and to check the metabolic condition of these cells after immunostimulation with phorbol 12-myristate 13-acetate (PMA). The viability of hemocytes (flow cytometry and luminescence techniques), their ultrastructure (transmission electron microscopy), and the antioxidant activity of hemolymph (superoxide dismutase; catalase; total antioxidant capacity; TAC) were analyzed. The results showed that, compared to copper, cadmium caused severe necrotic changes in hemocytes and impaired oxygen burst reactions and antioxidant responses, regardless of the exposure time. Copper induced degenerative changes only during short-term exposure, but its long-term intoxication did not impair the metabolic processes of hemocytes. Administration of PMA to spiders that were chronically exposed to either of the metals caused an increase in TAC levels in the hemolymph. Although the concentration of ATP in hemocytes was reduced relative to the control, the ADP/ATP ratio did not change, precluding a strong depletion of cellular energy resources. The metabolic condition of hemocytes stabilized with prolonged metal exposure, indicating the activation of defense mechanisms under operating stressors. © 2023 University of Silesia. Published by Informa UK Limited, trading as Taylor & Francis Group.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The purpose of this study was to evaluate the sensitivity of hemocytes of adult Steatoda grossa females to cadmium and copper administered orally for 4 weeks or 12 months, and to check the metabolic condition of these cells after immunostimulation with phorbol 12-myristate 13-acetate (PMA). The viability of hemocytes (flow cytometry and luminescence techniques), their ultrastructure (transmission electron microscopy), and the antioxidant activity of hemolymph (superoxide dismutase; catalase; total antioxidant capacity; TAC) were analyzed. The results showed that, compared to copper, cadmium caused severe necrotic changes in hemocytes and impaired oxygen burst reactions and antioxidant responses, regardless of the exposure time. Copper induced degenerative changes only during short-term exposure, but its long-term intoxication did not impair the metabolic processes of hemocytes. Administration of PMA to spiders that were chronically exposed to either of the metals caused an increase in TAC levels in the hemolymph. Although the concentration of ATP in hemocytes was reduced relative to the control, the ADP/ATP ratio did not change, precluding a strong depletion of cellular energy resources. The metabolic condition of hemocytes stabilized with prolonged metal exposure, indicating the activation of defense mechanisms under operating stressors. © 2023 University of Silesia. Published by Informa UK Limited, trading as Taylor & Francis Group.
2022
Szulińska, E.; Zakrzewski, D.; Kafel, A.; Gospodarek, J.; Rozpędek, K.; Zawisza-Raszka, A.
Level of oxidative stress for the land snail Cepaea nemoralis from aged and bioremediated soil contaminated with petroleum products Journal Article
In: Environmental Science and Pollution Research, vol. 29, no. 58, pp. 87218-87230, 2022, ISSN: 09441344, (1).
@article{2-s2.0-85133616898,
title = {Level of oxidative stress for the land snail Cepaea nemoralis from aged and bioremediated soil contaminated with petroleum products},
author = { E. Szulińska and D. Zakrzewski and A. Kafel and J. Gospodarek and K. Rozpędek and A. Zawisza-Raszka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85133616898&doi=10.1007%2fs11356-022-21854-y&partnerID=40&md5=e2d4edea792d6759cec9a3e9cc3cc979},
doi = {10.1007/s11356-022-21854-y},
issn = {09441344},
year = {2022},
date = {2022-01-01},
journal = {Environmental Science and Pollution Research},
volume = {29},
number = {58},
pages = {87218-87230},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Here, we investigated whether the widely distributed snail Cepaea nemoralis could be used as a suitable sentinel animal for assessing the effects of soil contaminants—petroleum oil derivatives—after years of soil ageing and treatment with a bacterial formulation. Oxidative stress was assessed in the foot and hepatopancreas of C. nemoralis L. exposed to soil contaminated with unleaded petrol, spent engine oil or diesel oil and bioremediated with a bacterial formulation (soil was used 2 years after contamination and bioremediation process). We measured total antioxidant capacity, catalase and glutathione transferase activity and concentrations of superoxide anions, hydrogen peroxide and protein carbonyls in the foot and hepatopancreas of snails after 2 and 4 weeks of treatment. The studied antioxidant responses appeared largely to be tissue and remediation process specific, while the concentrations of superoxide anions, hydrogen peroxide and protein carbonyls depended on time of exposure, tissue type and the type of contaminants, but mostly not on the remediation process. Generally, changes in the concentrations of superoxide anions, hydrogen peroxide and protein carbonyls in the hepatopancreas of snails seemed to be a suitable measure to assess the risk of animals exposed to soil contaminated with petroleum substances and used after many years of ageing and treatment with a microbial formulation. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Here, we investigated whether the widely distributed snail Cepaea nemoralis could be used as a suitable sentinel animal for assessing the effects of soil contaminants—petroleum oil derivatives—after years of soil ageing and treatment with a bacterial formulation. Oxidative stress was assessed in the foot and hepatopancreas of C. nemoralis L. exposed to soil contaminated with unleaded petrol, spent engine oil or diesel oil and bioremediated with a bacterial formulation (soil was used 2 years after contamination and bioremediation process). We measured total antioxidant capacity, catalase and glutathione transferase activity and concentrations of superoxide anions, hydrogen peroxide and protein carbonyls in the foot and hepatopancreas of snails after 2 and 4 weeks of treatment. The studied antioxidant responses appeared largely to be tissue and remediation process specific, while the concentrations of superoxide anions, hydrogen peroxide and protein carbonyls depended on time of exposure, tissue type and the type of contaminants, but mostly not on the remediation process. Generally, changes in the concentrations of superoxide anions, hydrogen peroxide and protein carbonyls in the hepatopancreas of snails seemed to be a suitable measure to assess the risk of animals exposed to soil contaminated with petroleum substances and used after many years of ageing and treatment with a microbial formulation. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Pompka, A.; Szulińska, E.; Kafel, A.
Starvation and cadmium affect energy reserves and oxidative stress in individuals of Spodoptera exigua Journal Article
In: Ecotoxicology, vol. 31, no. 9, pp. 1346-1355, 2022, ISSN: 09639292.
@article{2-s2.0-85139102911,
title = {Starvation and cadmium affect energy reserves and oxidative stress in individuals of Spodoptera exigua},
author = { A. Pompka and E. Szulińska and A. Kafel},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85139102911&doi=10.1007%2fs10646-022-02588-6&partnerID=40&md5=f65db545b650f7c318e80fa09fa4d176},
doi = {10.1007/s10646-022-02588-6},
issn = {09639292},
year = {2022},
date = {2022-01-01},
journal = {Ecotoxicology},
volume = {31},
number = {9},
pages = {1346-1355},
publisher = {Springer},
abstract = {Different factors, such as starvation and metal exposure, may affect development and cause oxidative stress in insects. Some host plants may contain a high concentration of cadmium due to their hyperaccumulating property. The negative effects of metals and hunger may be manifested by low availability of energetic substrates. This study aimed to assess whether the insect population with a history of long metal exposure may better manage metal stress or/and starvation at different developmental stages, with the use of energetic substrates. Two strains of Spodoptera exigua model organism were tested: control strain and cadmium strain (treated continuously for over 200 generations with subtoxic amounts of cadmium). The effects of different factors, individually and in combination, on the tested strains were assessed, first by determining the body weight of larvae and pupae and then by estimating the concentration of biomolecules (proteins; carbohydrates; lipids; or glycogen) in the 4th and 5th larval stages and in pupae, and the total antioxidant capacity and lipid peroxidation level in the 4th larval stage. Compared to control strain, cadmium strain individuals exhibited changes in the concentration of soluble carbohydrates and protein. This was partly related to earlier 1-day starvation. In particular, changes in carbohydrate concentration seemed to be a sensitive biomarker of metal stress, independent of the age of individuals and period of starvation. However, the increase in the total antioxidant capacity and the concentration of lipid peroxidation products in the 4th larval stage under the effect of cadmium was dependent on strain origin. © 2022, The Author(s).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Different factors, such as starvation and metal exposure, may affect development and cause oxidative stress in insects. Some host plants may contain a high concentration of cadmium due to their hyperaccumulating property. The negative effects of metals and hunger may be manifested by low availability of energetic substrates. This study aimed to assess whether the insect population with a history of long metal exposure may better manage metal stress or/and starvation at different developmental stages, with the use of energetic substrates. Two strains of Spodoptera exigua model organism were tested: control strain and cadmium strain (treated continuously for over 200 generations with subtoxic amounts of cadmium). The effects of different factors, individually and in combination, on the tested strains were assessed, first by determining the body weight of larvae and pupae and then by estimating the concentration of biomolecules (proteins; carbohydrates; lipids; or glycogen) in the 4th and 5th larval stages and in pupae, and the total antioxidant capacity and lipid peroxidation level in the 4th larval stage. Compared to control strain, cadmium strain individuals exhibited changes in the concentration of soluble carbohydrates and protein. This was partly related to earlier 1-day starvation. In particular, changes in carbohydrate concentration seemed to be a sensitive biomarker of metal stress, independent of the age of individuals and period of starvation. However, the increase in the total antioxidant capacity and the concentration of lipid peroxidation products in the 4th larval stage under the effect of cadmium was dependent on strain origin. © 2022, The Author(s).
Wiśniewska, K.; Rost-Roszkowska, M. M.; Homa, J.; Kasperkiewicz, K.; Surmiak-Stalmach, K.; Szulińska, E.; Wilczek, G.
In: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, vol. 252, 2022, ISSN: 15320456, (4).
@article{2-s2.0-85120968815,
title = {The effect of selected immunostimulants on hemocytes of the false black widow Steatoda grossa (Theridiidae) spiders under chronic exposition to cadmium},
author = { K. Wiśniewska and M.M. Rost-Roszkowska and J. Homa and K. Kasperkiewicz and K. Surmiak-Stalmach and E. Szulińska and G. Wilczek},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120968815&doi=10.1016%2fj.cbpc.2021.109221&partnerID=40&md5=a540ad597a3ee7233969b92b35b3305d},
doi = {10.1016/j.cbpc.2021.109221},
issn = {15320456},
year = {2022},
date = {2022-01-01},
journal = {Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology},
volume = {252},
publisher = {Elsevier Inc.},
abstract = {The aim of this study was to analyze whether, and to what extent, long–term exposure to cadmium, administered in sublethal concentrations by the oral route, caused changes in the immune potential of hemocytes in adult female Steatoda grossa spiders. We used artificial and natural immunostimulants, namely phorbol 12–myristate 13–acetate (PMA) and bacterial cell suspension based on Gram–positive (G+; Staphylococcus aureus) and Gram–negative (G−; Pseudomonas fluorescens) bacteria, to compare the status of hemocytes in nonstimulated individuals and those subjected to immunostimulation. After cadmium exposure, the percentage of small nongranular hemocytes in response to G+ cell suspension and PMA mitogen was decreased. Furthermore, in the cadmium–intoxicated spiders the percentage of plasmatocytes after immunostimulation remained lower compared to the complementary control group. Exposure to cadmium also induced several degenerative changes, including typical apoptotic and necrotic changes, in the analyzed types of cells. Immunostimulation by PMA mitogen and G+ bacterial suspension resulted in an increase in the number of cisterns in the rough endoplasmic reticulum of granulocytes, in both the control group and cadmium–treated individuals. These changes were accompanied with a low level of metallothioneins in hemolymph. Chronic cadmium exposure may significantly weaken the immune defense system of spiders during infections. © 2021 Elsevier Inc.},
note = {4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of this study was to analyze whether, and to what extent, long–term exposure to cadmium, administered in sublethal concentrations by the oral route, caused changes in the immune potential of hemocytes in adult female Steatoda grossa spiders. We used artificial and natural immunostimulants, namely phorbol 12–myristate 13–acetate (PMA) and bacterial cell suspension based on Gram–positive (G+; Staphylococcus aureus) and Gram–negative (G−; Pseudomonas fluorescens) bacteria, to compare the status of hemocytes in nonstimulated individuals and those subjected to immunostimulation. After cadmium exposure, the percentage of small nongranular hemocytes in response to G+ cell suspension and PMA mitogen was decreased. Furthermore, in the cadmium–intoxicated spiders the percentage of plasmatocytes after immunostimulation remained lower compared to the complementary control group. Exposure to cadmium also induced several degenerative changes, including typical apoptotic and necrotic changes, in the analyzed types of cells. Immunostimulation by PMA mitogen and G+ bacterial suspension resulted in an increase in the number of cisterns in the rough endoplasmic reticulum of granulocytes, in both the control group and cadmium–treated individuals. These changes were accompanied with a low level of metallothioneins in hemolymph. Chronic cadmium exposure may significantly weaken the immune defense system of spiders during infections. © 2021 Elsevier Inc.
Pompka, A.; Kafel, A.; Szulińska, E.
Avestia Publishing, 2022, ISSN: 23698128.
@proceedings{2-s2.0-85151327436,
title = {Starvation and Cadmium Affect Glutathione and Detoxifying Enzymes in Larvae of Spodoptera Exigua from Control and Cadmium Strain},
author = { A. Pompka and A. Kafel and E. Szulińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151327436&doi=10.11159%2ficepr22.150&partnerID=40&md5=cadd8f6a4fa9e4a4914abbe19bc0f807},
doi = {10.11159/icepr22.150},
issn = {23698128},
year = {2022},
date = {2022-01-01},
journal = {Proceedings of the World Congress on New Technologies},
publisher = {Avestia Publishing},
abstract = {Related to industrial and urban pollution, high load of cadmium in soil has been commonly noticed. That may cause an increase of metal amounts in herbivore food, host plant, among the others, in the hyperaccumulators of cadmium. Various environmental factors such as short period of starvation and metal exposure may affect differently detoxification processes in insects. The one of the indicative of metal toxicity may be oxidative stress, and enhanced cost of antioxidant defence. Glutathione is the main thiol in living cells. It plays an important role in antioxidant defense. About 90% is present in reduced form. Excessive oxidation can result in the formation of more disulfide, oxidized glutathione [1]. In a study carried out on Spodoptera exigua, the activity of the detoxifying enzyme glutathione S-transferase was checked in larvae from the first and second generation of these insects treated with zinc and cadmium added to the medium. Increase in the activity of the GST enzyme in the fat body and Malpighi tubules caused by zinc and its reduction under the influence of cadmium have been observed [2]. The presence of cadmium in the diet of the last instar S. exigua larvae resulted in lower larval survival rate and longer larval stages duration. Cadmium also contributed to an increase of total antioxidant capacity in the larval hemolymph [3]. The aim of this experiment was to compare cadmium and starvation effect on the 4th instars larvae, assessing their survival rate, the level of reduced glutathione (GSH), and activity of sensitive environmental biomarkers: Glutathione S-transferase (GST) and nonspecific esterase, between larvae of a common pest S. exigua originated from control and cadmium strains. They were exposed to cadmium during larval period and starving period (lasting 1 or 2 days during the 3rd instar period). It is appeared that the 4th instars larvae originated from cadmium strain characterised enhanced amount of GSH, higher activity of EST and GST, but only those which were not starved. Two days starving significantly decreased survival rate of the larvae. Sensitivity to two days lasting starvation, distinguished cadmium strain animals, for whom the lowering GSH level and drop in examined enzymes activity and the lowest number of survived animals were registered. A drop in GSH concentration was noticed for earlier starving larvae, exposed to cadmium and originated from control strain. It seems that in the case of esterases, lowering of the activity was growing with length of starvation period, similarly for control and cadmium strain animals. When, glutathione and glutathione related enzyme role seems differentiated the animals from control and cadmium strain, exposed to metal. © 2022, Avestia Publishing. All rights reserved.},
keywords = {},
pubstate = {published},
tppubtype = {proceedings}
}
Related to industrial and urban pollution, high load of cadmium in soil has been commonly noticed. That may cause an increase of metal amounts in herbivore food, host plant, among the others, in the hyperaccumulators of cadmium. Various environmental factors such as short period of starvation and metal exposure may affect differently detoxification processes in insects. The one of the indicative of metal toxicity may be oxidative stress, and enhanced cost of antioxidant defence. Glutathione is the main thiol in living cells. It plays an important role in antioxidant defense. About 90% is present in reduced form. Excessive oxidation can result in the formation of more disulfide, oxidized glutathione [1]. In a study carried out on Spodoptera exigua, the activity of the detoxifying enzyme glutathione S-transferase was checked in larvae from the first and second generation of these insects treated with zinc and cadmium added to the medium. Increase in the activity of the GST enzyme in the fat body and Malpighi tubules caused by zinc and its reduction under the influence of cadmium have been observed [2]. The presence of cadmium in the diet of the last instar S. exigua larvae resulted in lower larval survival rate and longer larval stages duration. Cadmium also contributed to an increase of total antioxidant capacity in the larval hemolymph [3]. The aim of this experiment was to compare cadmium and starvation effect on the 4th instars larvae, assessing their survival rate, the level of reduced glutathione (GSH), and activity of sensitive environmental biomarkers: Glutathione S-transferase (GST) and nonspecific esterase, between larvae of a common pest S. exigua originated from control and cadmium strains. They were exposed to cadmium during larval period and starving period (lasting 1 or 2 days during the 3rd instar period). It is appeared that the 4th instars larvae originated from cadmium strain characterised enhanced amount of GSH, higher activity of EST and GST, but only those which were not starved. Two days starving significantly decreased survival rate of the larvae. Sensitivity to two days lasting starvation, distinguished cadmium strain animals, for whom the lowering GSH level and drop in examined enzymes activity and the lowest number of survived animals were registered. A drop in GSH concentration was noticed for earlier starving larvae, exposed to cadmium and originated from control strain. It seems that in the case of esterases, lowering of the activity was growing with length of starvation period, similarly for control and cadmium strain animals. When, glutathione and glutathione related enzyme role seems differentiated the animals from control and cadmium strain, exposed to metal. © 2022, Avestia Publishing. All rights reserved.
2021
Francikowski, J.; Potrzebska, M.; Szulińska, E.; Tarnawska, M.; Rádai, Z.; Łozowski, B.; Baran, B.; Krzyżowski, M.
In: Scientific Reports, vol. 11, no. 1, 2021, ISSN: 20452322.
@article{2-s2.0-85117927480,
title = {The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus)},
author = { J. Francikowski and M. Potrzebska and E. Szulińska and M. Tarnawska and Z. Rádai and B. Łozowski and B. Baran and M. Krzyżowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117927480&doi=10.1038%2fs41598-021-00393-5&partnerID=40&md5=1c5df52d5f93052a5bf849a3c33f4229},
doi = {10.1038/s41598-021-00393-5},
issn = {20452322},
year = {2021},
date = {2021-01-01},
journal = {Scientific Reports},
volume = {11},
number = {1},
publisher = {Nature Research},
abstract = {Tryptophan (TRP) is one of the essential amino acids in the animal body. Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level. It has been shown to have a number of essential functions, such as in the production of other biologically active molecules. The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect—house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)—white eye. This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition. A parallel goal was to compare the response of both cricket strains to HMD. ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio. Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content. A decrease in food intake was also observed in the groups on HMD. However, no changes in imago body weight and water content were observed. The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain. At the same time, a stronger detrimental effect of fructose than of glucose was apparent. Sex was found to be a modulating factor in the response to HMD. © 2021, The Author(s).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Tryptophan (TRP) is one of the essential amino acids in the animal body. Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level. It has been shown to have a number of essential functions, such as in the production of other biologically active molecules. The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect—house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)—white eye. This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition. A parallel goal was to compare the response of both cricket strains to HMD. ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio. Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content. A decrease in food intake was also observed in the groups on HMD. However, no changes in imago body weight and water content were observed. The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain. At the same time, a stronger detrimental effect of fructose than of glucose was apparent. Sex was found to be a modulating factor in the response to HMD. © 2021, The Author(s).
2019
Wilczek, G.; Surmiak, K.; Wawszczak, B.; Sajewicz, M.; Kowalska, T.; Sindera, P.; Wiśniewska, K.; Szulińska, E.
In: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, vol. 224, 2019, ISSN: 15320456, (6).
@article{2-s2.0-85068519824,
title = {Effect of long-term cadmium and copper intoxication on the efficiency of ampullate silk glands in false black widow Steatoda grossa (Theridiidae) spiders},
author = { G. Wilczek and K. Surmiak and B. Wawszczak and M. Sajewicz and T. Kowalska and P. Sindera and K. Wiśniewska and E. Szulińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068519824&doi=10.1016%2fj.cbpc.2019.108564&partnerID=40&md5=22cba4b0aa5fad142326d2bf465ac3f6},
doi = {10.1016/j.cbpc.2019.108564},
issn = {15320456},
year = {2019},
date = {2019-01-01},
journal = {Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology},
volume = {224},
publisher = {Elsevier Inc.},
abstract = {The aim of the study was to compare cellular effects of xenobiotic cadmium and biogenic copper in ampullate silk glands of false black widow Steatoda grossa spider after long-term exposure via ingestion under laboratory conditions. Both the level of selected detoxification parameters (glutathione S-transferase; catalase; and the level of total antioxidant capacity) and degree of genotoxic changes (comet assay) were determined in the silk glands. Additionally the contents of selected amino acids (L-Ala; L-Pro; L-His; L-Phe; DL-Ile; and DL-Asn) in the hunting webs produced by spiders of this species were assessed. The ability of S. grossa females to accumulate cadmium was higher than that for copper. Long-term exposure of spiders to copper did not change the level of detoxification parameters, and the level of DNA damage in the cells of ampullate silk glands was also low. Cadmium had a stronger prooxidative and genotoxic effect than copper in the cells of the analyzed silk glands. However, regardless of the type of metal used, no significant changes in the level of amino acids in silk were found. The obtained results confirmed the effectiveness of metal neutralization mechanisms in the body of the studied spider species, which results in the protection of the function of ampullate silk glands. © 2019 Elsevier Inc.},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of the study was to compare cellular effects of xenobiotic cadmium and biogenic copper in ampullate silk glands of false black widow Steatoda grossa spider after long-term exposure via ingestion under laboratory conditions. Both the level of selected detoxification parameters (glutathione S-transferase; catalase; and the level of total antioxidant capacity) and degree of genotoxic changes (comet assay) were determined in the silk glands. Additionally the contents of selected amino acids (L-Ala; L-Pro; L-His; L-Phe; DL-Ile; and DL-Asn) in the hunting webs produced by spiders of this species were assessed. The ability of S. grossa females to accumulate cadmium was higher than that for copper. Long-term exposure of spiders to copper did not change the level of detoxification parameters, and the level of DNA damage in the cells of ampullate silk glands was also low. Cadmium had a stronger prooxidative and genotoxic effect than copper in the cells of the analyzed silk glands. However, regardless of the type of metal used, no significant changes in the level of amino acids in silk were found. The obtained results confirmed the effectiveness of metal neutralization mechanisms in the body of the studied spider species, which results in the protection of the function of ampullate silk glands. © 2019 Elsevier Inc.
2017
Wilczek, G.; Karcz, J.; Putko, A.; Kędziorski, A.; Wilczek, P.; Stalmach, M.; Szulińska, E.
In: Science of the Total Environment, vol. 586, pp. 1298-1307, 2017, ISSN: 00489697, (10).
@article{2-s2.0-85013411146,
title = {The effect of ingested cadmium on the calorific value and structural properties of hunting webs produced by Steatoda grossa (Theridiidae) spiders},
author = { G. Wilczek and J. Karcz and A. Putko and A. Kędziorski and P. Wilczek and M. Stalmach and E. Szulińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013411146&doi=10.1016%2fj.scitotenv.2017.02.143&partnerID=40&md5=66965df01015a23dde39659aca4caffc},
doi = {10.1016/j.scitotenv.2017.02.143},
issn = {00489697},
year = {2017},
date = {2017-01-01},
journal = {Science of the Total Environment},
volume = {586},
pages = {1298-1307},
publisher = {Elsevier B.V.},
abstract = {The study aimed to assess whether cadmium administered via ingestion to Steatoda grossa cobweb spiders (Theridiidae) affects the energy content and selected structural properties of the produced hunting webs. Cadmium content in webs was assessed with AAS and SEM X-ray microanalysis, while the diameters of silk fibers were estimated with SEM. The energy content of samples was measured in an oxygen micro-bomb calorimeter. Females and males showed different reactions to cadmium supplied through food. In comparison to females, males displayed higher metal concentrations in their bodies and hunting webs, however their calorific values and structural features were not significantly changed. Cadmium-treated females spun webs with smaller single-strand diameters and more frequent multi-stranded threads and invested 47% less energy in web production than the control individuals. It cannot be excluded that such a reduction in energy expenditure for web building in females resulted from energetically costly detoxifying reactions triggered in response to direct and indirect effects of cadmium toxicity. © 2017 Elsevier B.V.},
note = {10},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The study aimed to assess whether cadmium administered via ingestion to Steatoda grossa cobweb spiders (Theridiidae) affects the energy content and selected structural properties of the produced hunting webs. Cadmium content in webs was assessed with AAS and SEM X-ray microanalysis, while the diameters of silk fibers were estimated with SEM. The energy content of samples was measured in an oxygen micro-bomb calorimeter. Females and males showed different reactions to cadmium supplied through food. In comparison to females, males displayed higher metal concentrations in their bodies and hunting webs, however their calorific values and structural features were not significantly changed. Cadmium-treated females spun webs with smaller single-strand diameters and more frequent multi-stranded threads and invested 47% less energy in web production than the control individuals. It cannot be excluded that such a reduction in energy expenditure for web building in females resulted from energetically costly detoxifying reactions triggered in response to direct and indirect effects of cadmium toxicity. © 2017 Elsevier B.V.
2015
Stalmach, M.; Wilczek, G.; Homa, J.; Szulińska, E.
Antioxidative and immunological responses in the haemolymph of wolf spider Xerolycosa nemoralis (Lycosidae) exposed to starvation and dimethoate Journal Article
In: Environmental Pollution, vol. 206, pp. 551-559, 2015, ISSN: 02697491, (9).
@article{2-s2.0-84939833385,
title = {Antioxidative and immunological responses in the haemolymph of wolf spider Xerolycosa nemoralis (Lycosidae) exposed to starvation and dimethoate},
author = { M. Stalmach and G. Wilczek and J. Homa and E. Szulińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84939833385&doi=10.1016%2fj.envpol.2015.08.007&partnerID=40&md5=b124d047f71aab14f3d9422888ec39e8},
doi = {10.1016/j.envpol.2015.08.007},
issn = {02697491},
year = {2015},
date = {2015-01-01},
journal = {Environmental Pollution},
volume = {206},
pages = {551-559},
publisher = {Elsevier Ltd},
abstract = {The aim of this study was to assess the intensity of enzymatic antioxidative parameters [catalase (CAT); glutathione peroxidase (GSTPx); glutathione reductase (GR); total antioxidant capacity (TAC)] and percentage of high granularity cells as well as low to medium granularity cells in haemolymph of wolf spiders Xerolycosa nemoralis exposed to starvation and dimethoate under laboratory conditions. Only in starved males, haemolymph included a lower percentage of high granularity cells, accompanied by high activity of CAT and GSTPx, than in the control. Exposure of males to dimethoate increased CAT activity, after single application, and significantly enhanced GR activity, after five-time application. In females, five-time contact with dimethoate elevated the percentage of high granularity cells. As in comparison to females, male X. nemoralis were more sensitive to the applied stressing factors, it may be concluded that in natural conditions both food deficiency and chemical stress may diminish the immune response of their organisms. © 2015 Elsevier Ltd.},
note = {9},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of this study was to assess the intensity of enzymatic antioxidative parameters [catalase (CAT); glutathione peroxidase (GSTPx); glutathione reductase (GR); total antioxidant capacity (TAC)] and percentage of high granularity cells as well as low to medium granularity cells in haemolymph of wolf spiders Xerolycosa nemoralis exposed to starvation and dimethoate under laboratory conditions. Only in starved males, haemolymph included a lower percentage of high granularity cells, accompanied by high activity of CAT and GSTPx, than in the control. Exposure of males to dimethoate increased CAT activity, after single application, and significantly enhanced GR activity, after five-time application. In females, five-time contact with dimethoate elevated the percentage of high granularity cells. As in comparison to females, male X. nemoralis were more sensitive to the applied stressing factors, it may be concluded that in natural conditions both food deficiency and chemical stress may diminish the immune response of their organisms. © 2015 Elsevier Ltd.
2014
Babczyńska, A.; Binkowski, M.; Bednarek, A. W.; Ogierman, S.; Cibura, D.; Migula, P.; Wilczek, G.; Szulińska, E.
X-ray microtomography for imaging of developing spiders inside egg cocoons Journal Article
In: Arthropod Structure and Development, vol. 43, no. 6, pp. 595-603, 2014, ISSN: 14678039, (1).
@article{2-s2.0-84908573895,
title = {X-ray microtomography for imaging of developing spiders inside egg cocoons},
author = { A. Babczyńska and M. Binkowski and A.W. Bednarek and S. Ogierman and D. Cibura and P. Migula and G. Wilczek and E. Szulińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908573895&doi=10.1016%2fj.asd.2014.09.002&partnerID=40&md5=8f57e2706ecf9e444ced1c2efa9f0a1c},
doi = {10.1016/j.asd.2014.09.002},
issn = {14678039},
year = {2014},
date = {2014-01-01},
journal = {Arthropod Structure and Development},
volume = {43},
number = {6},
pages = {595-603},
publisher = {Elsevier Ltd},
abstract = {Embryogenesis is especially sensitive to external factors. The changes in its course are often used as biomarkers of environmental impact. Since spider embryogenesis takes place inside cocoons, it is crucial to find a reliable tool to analyze this developmental phase with no intrusion into the cocoons. The aim of this study was to verify the efficacy of X-ray microtomography for non-invasive analysis of embryonic morphology and egg quantity in the cocoons of Xerolycosa nemoralis and Agelena labyrinthica from polluted and reference sites. X-ray microtomography slice images as well as 3D images and animations obtained from digital visualization of those slides were used to study the morphology of embryos and egg arrangement in the cocoons. Any disorders in embryogenesis or malformation of embryos in relation to site of origin have not been found, but inside an egg cocoon of X.nemoralis from the polluted site embryos differing form each other by one developmental stage were identified. Egg calculation revealed a K- reproductive strategy of X.nemoralis from polluted sites. Finally, future prospects and benefits, and weaknessess of this method for the study of spider cocoons have presented. © 2014 Elsevier Ltd.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Embryogenesis is especially sensitive to external factors. The changes in its course are often used as biomarkers of environmental impact. Since spider embryogenesis takes place inside cocoons, it is crucial to find a reliable tool to analyze this developmental phase with no intrusion into the cocoons. The aim of this study was to verify the efficacy of X-ray microtomography for non-invasive analysis of embryonic morphology and egg quantity in the cocoons of Xerolycosa nemoralis and Agelena labyrinthica from polluted and reference sites. X-ray microtomography slice images as well as 3D images and animations obtained from digital visualization of those slides were used to study the morphology of embryos and egg arrangement in the cocoons. Any disorders in embryogenesis or malformation of embryos in relation to site of origin have not been found, but inside an egg cocoon of X.nemoralis from the polluted site embryos differing form each other by one developmental stage were identified. Egg calculation revealed a K- reproductive strategy of X.nemoralis from polluted sites. Finally, future prospects and benefits, and weaknessess of this method for the study of spider cocoons have presented. © 2014 Elsevier Ltd.
Wilczek, G.; Rost-Roszkowska, M. M.; Wilczek, P.; Babczyńska, A.; Szulińska, E.; Sonakowska, L.; Marek-Swedzioł, M.
In: Ecotoxicology and Environmental Safety, vol. 101, no. 1, pp. 157-167, 2014, ISSN: 01476513, (28).
@article{2-s2.0-84893176120,
title = {Apoptotic and necrotic changes in the midgut glands of the wolf spider Xerolycosa nemoralis (Lycosidae) in response to starvation and dimethoate exposure},
author = { G. Wilczek and M.M. Rost-Roszkowska and P. Wilczek and A. Babczyńska and E. Szulińska and L. Sonakowska and M. Marek-Swedzioł},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84893176120&doi=10.1016%2fj.ecoenv.2013.09.034&partnerID=40&md5=b3fbd74af84e674bc32ed8f57abf0e60},
doi = {10.1016/j.ecoenv.2013.09.034},
issn = {01476513},
year = {2014},
date = {2014-01-01},
journal = {Ecotoxicology and Environmental Safety},
volume = {101},
number = {1},
pages = {157-167},
abstract = {In the present study, the intensity of degenerative changes (apoptosis; necrosis) in the cells of the midgut glands of male and female wolf spiders, Xerolycosa nemoralis (Lycosidae), exposed to natural (starvation) and anthropogenic (the organophosphorous pesticide dimethoate) stressors under laboratory conditions were compared. The spiders were collected from two differentially polluted sites, both located in southern Poland: Katowice-Welnowiec, which is heavily polluted with metals, and Pilica, the reference site. Starvation and dimethoate treatment resulted in enhancement of apoptotic and necrotic changes in the midgut glands of the spiders. The frequency of degenerative changes in starving individuals was twice as high as in the specimens intoxicated with dimethoate. The percentage of apoptotic and necrotic cells was higher in starving males than in starving females. A high intensity of necrotic changes, together with increased Cas-3 like activity and a greater percentage of cells with depolarized mitochondria, were typical of starving males from the polluted site. The cell death indices observed in females depended more strongly on the type of stressor than on previous preexposure to pollutants. © 2013 Elsevier Inc.},
note = {28},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In the present study, the intensity of degenerative changes (apoptosis; necrosis) in the cells of the midgut glands of male and female wolf spiders, Xerolycosa nemoralis (Lycosidae), exposed to natural (starvation) and anthropogenic (the organophosphorous pesticide dimethoate) stressors under laboratory conditions were compared. The spiders were collected from two differentially polluted sites, both located in southern Poland: Katowice-Welnowiec, which is heavily polluted with metals, and Pilica, the reference site. Starvation and dimethoate treatment resulted in enhancement of apoptotic and necrotic changes in the midgut glands of the spiders. The frequency of degenerative changes in starving individuals was twice as high as in the specimens intoxicated with dimethoate. The percentage of apoptotic and necrotic cells was higher in starving males than in starving females. A high intensity of necrotic changes, together with increased Cas-3 like activity and a greater percentage of cells with depolarized mitochondria, were typical of starving males from the polluted site. The cell death indices observed in females depended more strongly on the type of stressor than on previous preexposure to pollutants. © 2013 Elsevier Inc.
Kafel, A.; Rozpędek, K.; Szulińska, E.; Zawisza-Raszka, A.; Migula, P.
The effects of cadmium or zinc multigenerational exposure on metal tolerance of Spodoptera exigua (Lepidoptera: Noctuidae) Journal Article
In: Environmental Science and Pollution Research, vol. 21, no. 6, pp. 4705-4715, 2014, ISSN: 09441344, (35).
@article{2-s2.0-84895869162,
title = {The effects of cadmium or zinc multigenerational exposure on metal tolerance of Spodoptera exigua (Lepidoptera: Noctuidae)},
author = { A. Kafel and K. Rozpędek and E. Szulińska and A. Zawisza-Raszka and P. Migula},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84895869162&doi=10.1007%2fs11356-013-2409-z&partnerID=40&md5=6cab772a75d4c8278ab7dfd94b0558c2},
doi = {10.1007/s11356-013-2409-z},
issn = {09441344},
year = {2014},
date = {2014-01-01},
journal = {Environmental Science and Pollution Research},
volume = {21},
number = {6},
pages = {4705-4715},
publisher = {Ecomed Publishers},
abstract = {The effects of ten generational zinc or cadmium pre-exposure on metal tolerance among beet armyworm Spodoptera exigua individuals were compared. These effects were assessed in animals from the 11th generation, reared on a diet either uncontaminated or contaminated with metal (cadmium or zinc). The survival rate of larvae and the degree of metal accumulation (in larvae; pupae and moths; among larval organs: gut and fat body) were analysed. Catalase, superoxide dismutase and glutathione transferase activity in larval organs of individuals subjected to different metal treatments were also measured. Animals transferred from control rearing to metals (cadmium or zinc) in the 11th generation, as well as those from multigenerational zinc treatment, but not from multigenerational cadmium treatment, had a significantly lower survival rate than control animals. Insects from the groups with the high metal treatment had high bioaccumulation factors (above 3.7 and 2.3 following cadmium and zinc; respectively). Cadmium (but not zinc) pre-exposure had a significant effect on metal accumulation in larvae. Multigenerational metal pre-exposure seemed to have mainly a negative effect on glutathione transferase activity in the gut of larvae from the 11th generation, in the case of the individuals exposed to metal other than that used in pre-exposure treatment or kept in control conditions. However, in the case of zinc pre-exposure, such effect was only apparent when zinc was replaced by cadmium. The long-term effect of cadmium on catalase activity in larvae was found. © 2013 The Author(s).},
note = {35},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The effects of ten generational zinc or cadmium pre-exposure on metal tolerance among beet armyworm Spodoptera exigua individuals were compared. These effects were assessed in animals from the 11th generation, reared on a diet either uncontaminated or contaminated with metal (cadmium or zinc). The survival rate of larvae and the degree of metal accumulation (in larvae; pupae and moths; among larval organs: gut and fat body) were analysed. Catalase, superoxide dismutase and glutathione transferase activity in larval organs of individuals subjected to different metal treatments were also measured. Animals transferred from control rearing to metals (cadmium or zinc) in the 11th generation, as well as those from multigenerational zinc treatment, but not from multigenerational cadmium treatment, had a significantly lower survival rate than control animals. Insects from the groups with the high metal treatment had high bioaccumulation factors (above 3.7 and 2.3 following cadmium and zinc; respectively). Cadmium (but not zinc) pre-exposure had a significant effect on metal accumulation in larvae. Multigenerational metal pre-exposure seemed to have mainly a negative effect on glutathione transferase activity in the gut of larvae from the 11th generation, in the case of the individuals exposed to metal other than that used in pre-exposure treatment or kept in control conditions. However, in the case of zinc pre-exposure, such effect was only apparent when zinc was replaced by cadmium. The long-term effect of cadmium on catalase activity in larvae was found. © 2013 The Author(s).
2013
Babczyńska, A.; Wilczek, G.; Szulińska, E.; Migula, P.; Binkowski, M.
EDP Sciences, vol. 1, 2013, ISSN: 22671242.
@proceedings{2-s2.0-85016521021,
title = {The development al potential of the embryos of Wolf spider Xerolycosa nemoralis from areas variously burdened with metals},
author = { A. Babczyńska and G. Wilczek and E. Szulińska and P. Migula and M. Binkowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016521021&doi=10.1051%2fe3sconf%2f20130134006&partnerID=40&md5=9ee625ae2f065e0e414f08795088a92c},
doi = {10.1051/e3sconf/20130134006},
issn = {22671242},
year = {2013},
date = {2013-01-01},
journal = {E3S Web of Conferences},
volume = {1},
publisher = {EDP Sciences},
abstract = {The aim of this study was to assess and describe the developmental potential of the embryos of the Wolf spider X. nemoralis collected from the reference site (Pilica) and a site heavily polluted with metals (Welnowiec) (southern Poland). For the analyses of the progress of development the embryos the computer microtomography was used. Adenylate concentration was measured using luminometric method. The macroscopic observations of the development of individual embryos revealed significant differences in the duration of embryogenesis (14;5 ±0.5 days in Pilica vs 16.5±0.53 in Welnowiec). The computer microtomography analyses enabled us to describe the geometry of the eggs inside the cocoon as well as to see the morphology of embryos inside the cocoon and to recognize selected developmental stages of the embryos. The concentration of cadmium in embryos in Welnowiec was significantly lower than in the embryos from the reference site, while the concentration of copper in the embryos from Pilica was significantly, 4 times lower than in Wełnowiec. The energy status indices (ATP concentration and ADP/ATP ratio) did not differ significantly in the embryos from the two sites. © Owned by the authors, published by EDP Sciences, 2013.},
keywords = {},
pubstate = {published},
tppubtype = {proceedings}
}
The aim of this study was to assess and describe the developmental potential of the embryos of the Wolf spider X. nemoralis collected from the reference site (Pilica) and a site heavily polluted with metals (Welnowiec) (southern Poland). For the analyses of the progress of development the embryos the computer microtomography was used. Adenylate concentration was measured using luminometric method. The macroscopic observations of the development of individual embryos revealed significant differences in the duration of embryogenesis (14;5 ±0.5 days in Pilica vs 16.5±0.53 in Welnowiec). The computer microtomography analyses enabled us to describe the geometry of the eggs inside the cocoon as well as to see the morphology of embryos inside the cocoon and to recognize selected developmental stages of the embryos. The concentration of cadmium in embryos in Welnowiec was significantly lower than in the embryos from the reference site, while the concentration of copper in the embryos from Pilica was significantly, 4 times lower than in Wełnowiec. The energy status indices (ATP concentration and ADP/ATP ratio) did not differ significantly in the embryos from the two sites. © Owned by the authors, published by EDP Sciences, 2013.
2012
Babczyńska, A.; Wilczek, G.; Szulińska, E.; Kędziorski, A.; Franiel, I.; Migula, P.
The reproductive potential of the spiders Agelena labyrinthica and Xerolycosa nemoralis from areas contaminated with metals Journal Article
In: Science of the Total Environment, vol. 435-436, pp. 374-379, 2012, ISSN: 00489697, (10).
@article{2-s2.0-84864504690,
title = {The reproductive potential of the spiders Agelena labyrinthica and Xerolycosa nemoralis from areas contaminated with metals},
author = { A. Babczyńska and G. Wilczek and E. Szulińska and A. Kędziorski and I. Franiel and P. Migula},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864504690&doi=10.1016%2fj.scitotenv.2012.06.102&partnerID=40&md5=79b4edc840dd10b8ed2c4b4feb3386e5},
doi = {10.1016/j.scitotenv.2012.06.102},
issn = {00489697},
year = {2012},
date = {2012-01-01},
journal = {Science of the Total Environment},
volume = {435-436},
pages = {374-379},
abstract = {Spiders successfully colonize industrially contaminated environments and maintain relatively stable populations. The aim of this study was to explain the reproductive strategies of two spider species, Xerolycosa nemoralis (an actively hunting; sit-and-pursue predator) and Agelena labyrinthica (a web. -building; sit-and-wait predator), between contaminated and uncontaminated sites. Spiders were collected from a reference site (Pilica) and two contaminated sites (Olkusz and Welnowiec). The amount of energy allocated to the eggs and the number of eggs and hatchlings as well as the hatching success were compared. Wolf spiders from the contaminated sites produced fewer but relatively energy-rich eggs, whereas web-building spiders invested their energy in the production of a higher number of less energy-rich eggs. The comparisons of the hatching percentages suggested that in the contaminated habitats, X. nemoralis achieve a hatching success similar to or higher than that of the reference population at Pilica. A. labyrinthica in the contaminated sites invested a larger amount of energy in eggs than at the reference site, but the hatching success found for this species in the contaminated areas was lower than that found at the reference site. © 2012 Elsevier B.V.},
note = {10},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Spiders successfully colonize industrially contaminated environments and maintain relatively stable populations. The aim of this study was to explain the reproductive strategies of two spider species, Xerolycosa nemoralis (an actively hunting; sit-and-pursue predator) and Agelena labyrinthica (a web. -building; sit-and-wait predator), between contaminated and uncontaminated sites. Spiders were collected from a reference site (Pilica) and two contaminated sites (Olkusz and Welnowiec). The amount of energy allocated to the eggs and the number of eggs and hatchlings as well as the hatching success were compared. Wolf spiders from the contaminated sites produced fewer but relatively energy-rich eggs, whereas web-building spiders invested their energy in the production of a higher number of less energy-rich eggs. The comparisons of the hatching percentages suggested that in the contaminated habitats, X. nemoralis achieve a hatching success similar to or higher than that of the reference population at Pilica. A. labyrinthica in the contaminated sites invested a larger amount of energy in eggs than at the reference site, but the hatching success found for this species in the contaminated areas was lower than that found at the reference site. © 2012 Elsevier B.V.
Kafel, A.; Zawisza-Raszka, A.; Szulińska, E.
In: Environmental Pollution, vol. 162, pp. 8-14, 2012, ISSN: 02697491, (48).
@article{2-s2.0-80755169293,
title = {Effects of multigenerational cadmium exposure of insects (Spodoptera exigua larvae) on anti-oxidant response in haemolymph and developmental parameters},
author = { A. Kafel and A. Zawisza-Raszka and E. Szulińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80755169293&doi=10.1016%2fj.envpol.2011.09.034&partnerID=40&md5=b29311363a86333d86159b6d45a44a7e},
doi = {10.1016/j.envpol.2011.09.034},
issn = {02697491},
year = {2012},
date = {2012-01-01},
journal = {Environmental Pollution},
volume = {162},
pages = {8-14},
abstract = {Biochemical and organismal indices of metal tolerance were studied in Spodoptera exigua exposed to a cadmium-contaminated diet for one or many (33 or 61) generations. Reduced and oxidised glutathione, protein thiols, total anti-oxidant capacity level, glutathione transferase activity, and Cd accumulation were assayed in the haemolymph of the last instar larvae. The cadmium concentration in the whole larval body as well as larval survival, larval duration time and last instar body weight were also measured. Elevated cadmium concentration in the whole body, higher mortality and longer duration of the larval stage in one-generation exposed insects in comparison with those exposed for many generations suggest that metal tolerance builds over time. For the larvae from multigeneration metal treatment, the higher cadmium concentration in larval haemolymph positively correlated with glutathione oxidation and total anti-oxidant capacity. One-generation exposed insects had lower metal concentration in haemolymph than did 33-generation exposed insects. © 2011 Elsevier Ltd. All rights reserved.},
note = {48},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Biochemical and organismal indices of metal tolerance were studied in Spodoptera exigua exposed to a cadmium-contaminated diet for one or many (33 or 61) generations. Reduced and oxidised glutathione, protein thiols, total anti-oxidant capacity level, glutathione transferase activity, and Cd accumulation were assayed in the haemolymph of the last instar larvae. The cadmium concentration in the whole larval body as well as larval survival, larval duration time and last instar body weight were also measured. Elevated cadmium concentration in the whole body, higher mortality and longer duration of the larval stage in one-generation exposed insects in comparison with those exposed for many generations suggest that metal tolerance builds over time. For the larvae from multigeneration metal treatment, the higher cadmium concentration in larval haemolymph positively correlated with glutathione oxidation and total anti-oxidant capacity. One-generation exposed insects had lower metal concentration in haemolymph than did 33-generation exposed insects. © 2011 Elsevier Ltd. All rights reserved.
2011
Babczyńska, A.; Wilczek, G.; Szulińska, E.; Franiel, I.
Quantitative immunodetection of metallothioneins in relation to metals concentration in spiders from variously polluted areas Journal Article
In: Ecotoxicology and Environmental Safety, vol. 74, no. 6, pp. 1498-1503, 2011, ISSN: 01476513, (20).
@article{2-s2.0-79961129533,
title = {Quantitative immunodetection of metallothioneins in relation to metals concentration in spiders from variously polluted areas},
author = { A. Babczyńska and G. Wilczek and E. Szulińska and I. Franiel},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79961129533&doi=10.1016%2fj.ecoenv.2011.05.019&partnerID=40&md5=5f2442c3abb029fe1da769a9913cbba8},
doi = {10.1016/j.ecoenv.2011.05.019},
issn = {01476513},
year = {2011},
date = {2011-01-01},
journal = {Ecotoxicology and Environmental Safety},
volume = {74},
number = {6},
pages = {1498-1503},
abstract = {Spiders inhabiting post industrial environments, such as waste heaps or ore-bearing areas, are exposed to high concentrations of metals, accumulated in the body of their prey and transferred along food chains. Therefore spiders are pressed to develop metal-neutralization strategies. Low-molecular, multifunction proteins: metallothioneins (MTs), often postulated as biomarkers of metal exposure, are known to bind metals and thus protect organisms against their toxic effects. Yet the proteins are still not well recognized in spiders. The aim of this study was to assess, by immunodetection method, ELISA, the concentration of metallothioneins in adult females of three web building spider species: Araneus diadematus (Araneidae), Agelena labyrinthica (Agelenidae) and Linyphia triangularis (Linyphiidae) from three variously polluted areas in southern Poland: Olkusz, ore-bearing post industrial site; Katowice-Welnowiec: post metallurgic waste heap, Pilica: the reference, rural, area. The concentration of metallothioneins has been analyzed in relation to the metal concentration in spiders body. The study gives the evidence that metallothioneins are reliably detectable by means of ELISA technique. The analysis of results obtained shows a strong species-dependence of the MTs level. Positive correlations between MTs concentration and metal body burden (mainly Zn and Pb) were found. This suggests that the proteins play an important role in the neutralization and regulation of metal ions in spiders. The same correlation indicate the possibility to consider MTs in spiders as biomarkers of metal exposure and effects. However, the species specificity as well as metal characteristics should be taken under account. © 2011 Elsevier Inc.},
note = {20},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Spiders inhabiting post industrial environments, such as waste heaps or ore-bearing areas, are exposed to high concentrations of metals, accumulated in the body of their prey and transferred along food chains. Therefore spiders are pressed to develop metal-neutralization strategies. Low-molecular, multifunction proteins: metallothioneins (MTs), often postulated as biomarkers of metal exposure, are known to bind metals and thus protect organisms against their toxic effects. Yet the proteins are still not well recognized in spiders. The aim of this study was to assess, by immunodetection method, ELISA, the concentration of metallothioneins in adult females of three web building spider species: Araneus diadematus (Araneidae), Agelena labyrinthica (Agelenidae) and Linyphia triangularis (Linyphiidae) from three variously polluted areas in southern Poland: Olkusz, ore-bearing post industrial site; Katowice-Welnowiec: post metallurgic waste heap, Pilica: the reference, rural, area. The concentration of metallothioneins has been analyzed in relation to the metal concentration in spiders body. The study gives the evidence that metallothioneins are reliably detectable by means of ELISA technique. The analysis of results obtained shows a strong species-dependence of the MTs level. Positive correlations between MTs concentration and metal body burden (mainly Zn and Pb) were found. This suggests that the proteins play an important role in the neutralization and regulation of metal ions in spiders. The same correlation indicate the possibility to consider MTs in spiders as biomarkers of metal exposure and effects. However, the species specificity as well as metal characteristics should be taken under account. © 2011 Elsevier Inc.
Babczyńska, A.; Wilczek, G.; Wilczek, P.; Szulińska, E.; Witas, I.
In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, vol. 154, no. 3, pp. 161-171, 2011, ISSN: 15320456, (33).
@article{2-s2.0-79960955274,
title = {Metallothioneins and energy budget indices in cadmium and copper exposed spiders Agelena labyrinthica in relation to their developmental stage, gender and origin},
author = { A. Babczyńska and G. Wilczek and P. Wilczek and E. Szulińska and I. Witas},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960955274&doi=10.1016%2fj.cbpc.2011.05.001&partnerID=40&md5=48cd98709a9efeedcae519104b3090ec},
doi = {10.1016/j.cbpc.2011.05.001},
issn = {15320456},
year = {2011},
date = {2011-01-01},
journal = {Comparative Biochemistry and Physiology - C Toxicology and Pharmacology},
volume = {154},
number = {3},
pages = {161-171},
publisher = {Elsevier Inc.},
abstract = {The aim of our studies was to explain the role of metallothioneins (MTs) in the neutralization of excessive amounts of metals (essential: copper (Cu) and toxic: cadmium (Cd)) and to describe the energy status in metal-exposed spiders Agelena labyrinthica in relation to its developmental stage, gender and origin. Juvenile, female and male spiders were collected from three variously polluted habitats, transferred to the laboratory and exposed to the metals in their diet. Cu and Cd accumulation in the body and exuviae, bioaccumulation factor, percentage of metallothionein positive cells, MT concentration, percentage of cells with depolarized mitochondria, ATP concentration and ADP/ATP ratio were measured and calculated. Cu appeared to be regulated and its excess is eliminated via, among others, the molting process, while Cd was rather accumulated by the spiders. The level of MTs increased significantly mainly in females exposed to both metals, irrespectively of the pollution degree of their site of origin, indicating a defensive role of the proteins. In general, even if both the MT level and the energy status indices were positively correlated with Cd and Cu concentrations in the spider body, the energy status of A. labyrinthica did not seem disturbed. © 2011 Elsevier Inc.},
note = {33},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of our studies was to explain the role of metallothioneins (MTs) in the neutralization of excessive amounts of metals (essential: copper (Cu) and toxic: cadmium (Cd)) and to describe the energy status in metal-exposed spiders Agelena labyrinthica in relation to its developmental stage, gender and origin. Juvenile, female and male spiders were collected from three variously polluted habitats, transferred to the laboratory and exposed to the metals in their diet. Cu and Cd accumulation in the body and exuviae, bioaccumulation factor, percentage of metallothionein positive cells, MT concentration, percentage of cells with depolarized mitochondria, ATP concentration and ADP/ATP ratio were measured and calculated. Cu appeared to be regulated and its excess is eliminated via, among others, the molting process, while Cd was rather accumulated by the spiders. The level of MTs increased significantly mainly in females exposed to both metals, irrespectively of the pollution degree of their site of origin, indicating a defensive role of the proteins. In general, even if both the MT level and the energy status indices were positively correlated with Cd and Cu concentrations in the spider body, the energy status of A. labyrinthica did not seem disturbed. © 2011 Elsevier Inc.
2003
Doleżych, B.; Szulińska, E.
Selenium modifies glutathione peroxidase activity and glutathione concentration in mice exposed to ozone-provoked oxidative stress Journal Article
In: Journal of Trace Elements in Medicine and Biology, vol. 17, no. 2, pp. 133-137, 2003, ISSN: 0946672X, (6).
@article{2-s2.0-0141592536,
title = {Selenium modifies glutathione peroxidase activity and glutathione concentration in mice exposed to ozone-provoked oxidative stress},
author = { B. Doleżych and E. Szulińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141592536&doi=10.1016%2fS0946-672X%2803%2980009-3&partnerID=40&md5=cc1d2ec02db3c6d52c7cc3aeece527c0},
doi = {10.1016/S0946-672X(03)80009-3},
issn = {0946672X},
year = {2003},
date = {2003-01-01},
journal = {Journal of Trace Elements in Medicine and Biology},
volume = {17},
number = {2},
pages = {133-137},
publisher = {Elsevier GmbH},
abstract = {The aim of this study was to show the direct effect of selenium on glutathione peroxidase (GSH-Px) activity and GSH/GSSG concentrations in 3- and 6-month-old mice. An ozone-oxygen mixture was used to provoke an oxygen stress. To measure the Se-effect mice were gavaged with sodium selenite. GSH-Px activity and total glutathione concentrations were determined in serum and in the postnuclear fraction of liver and lungs. Additionally glutathione concentrations were determined in whole blood. Both ozone and selenium, administered separately, reduced GSH-Px activity in lungs of 6-month-old animals, while in young mice an opposite effect of Se was observed. Ozone administered jointly with Se did not influence GSH-Px activity in 6-month-old mice, while in young, 3-month-old mice, a stimulatory effect in lungs was observed. There were no significant changes in GSH-Px activity in the liver of 6-month-old mice, but the stimulatory effect occurred in young mice treated with Se and Se & ozone jointly. In young mice, ozone (also ozone with Se) augmented glutathione concentrations. The response to ozone and selenium strictly depended on age and the antagonism between selenium and ozone was observed only in a few cases.},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of this study was to show the direct effect of selenium on glutathione peroxidase (GSH-Px) activity and GSH/GSSG concentrations in 3- and 6-month-old mice. An ozone-oxygen mixture was used to provoke an oxygen stress. To measure the Se-effect mice were gavaged with sodium selenite. GSH-Px activity and total glutathione concentrations were determined in serum and in the postnuclear fraction of liver and lungs. Additionally glutathione concentrations were determined in whole blood. Both ozone and selenium, administered separately, reduced GSH-Px activity in lungs of 6-month-old animals, while in young mice an opposite effect of Se was observed. Ozone administered jointly with Se did not influence GSH-Px activity in 6-month-old mice, while in young, 3-month-old mice, a stimulatory effect in lungs was observed. There were no significant changes in GSH-Px activity in the liver of 6-month-old mice, but the stimulatory effect occurred in young mice treated with Se and Se & ozone jointly. In young mice, ozone (also ozone with Se) augmented glutathione concentrations. The response to ozone and selenium strictly depended on age and the antagonism between selenium and ozone was observed only in a few cases.
Kafel, A.; Bednarska, K.; Augustyniak, M.; Witas, I.; Szulińska, E.
Activity of glutathione S-transferase in Spodoptera exigua larvae exposed to cadmium and zinc in two subsequent generations Journal Article
In: Environment International, vol. 28, no. 8, pp. 683-686, 2003, ISSN: 01604120, (23).
@article{2-s2.0-0037340581,
title = {Activity of glutathione S-transferase in Spodoptera exigua larvae exposed to cadmium and zinc in two subsequent generations},
author = { A. Kafel and K. Bednarska and M. Augustyniak and I. Witas and E. Szulińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037340581&doi=10.1016%2fS0160-4120%2802%2900111-3&partnerID=40&md5=8082a67a14755b7f7ed59ab4571f1c33},
doi = {10.1016/S0160-4120(02)00111-3},
issn = {01604120},
year = {2003},
date = {2003-01-01},
journal = {Environment International},
volume = {28},
number = {8},
pages = {683-686},
publisher = {Elsevier Ltd},
abstract = {The aim of our study was to establish changes in activity of important in detoxification enzyme - glutathione S-transferase (GST): in alimentary tract, fat body and Malpighian tubules of Spodoptera exigua larvae being under cadmium and zinc exposure through the first as well as the second generation. There was registered enhancement of the enzyme activity in the fat body and the Malpighian tubules caused by zinc as well as its decrease in the Malpighian tubules under cadmium action. Amounts of metals in the alimentary tract were either several times higher than in the diet ingested by larvae or than in the fat body. Metal concentration in the fat body correlated with the level of the enzyme activity (positive correlation for zinc and negative for cadmium). The effect of metal action differentiated dependently on time exposition. © 2002 Elsevier Science Ltd. All rights reserved.},
note = {23},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of our study was to establish changes in activity of important in detoxification enzyme - glutathione S-transferase (GST): in alimentary tract, fat body and Malpighian tubules of Spodoptera exigua larvae being under cadmium and zinc exposure through the first as well as the second generation. There was registered enhancement of the enzyme activity in the fat body and the Malpighian tubules caused by zinc as well as its decrease in the Malpighian tubules under cadmium action. Amounts of metals in the alimentary tract were either several times higher than in the diet ingested by larvae or than in the fat body. Metal concentration in the fat body correlated with the level of the enzyme activity (positive correlation for zinc and negative for cadmium). The effect of metal action differentiated dependently on time exposition. © 2002 Elsevier Science Ltd. All rights reserved.
1996
Kędziorski, A.; Nakonieczny, M.; Świerczek, E.; Szulińska, E.
Cadmium-selenium antagonism and detoxifying enzymes in insects Journal Article
In: Fresenius' Journal of Analytical Chemistry, vol. 354, no. 5-6, pp. 571-575, 1996, ISSN: 09370633, (4).
@article{2-s2.0-0030369820,
title = {Cadmium-selenium antagonism and detoxifying enzymes in insects},
author = { A. Kędziorski and M. Nakonieczny and E. Świerczek and E. Szulińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030369820&partnerID=40&md5=18eda2046c9653fdc406c19866c86013},
issn = {09370633},
year = {1996},
date = {1996-01-01},
journal = {Fresenius' Journal of Analytical Chemistry},
volume = {354},
number = {5-6},
pages = {571-575},
abstract = {Activities of non-specific carboxylesterases (CarE) have been studied in adult males of hissing cockroach Gromphadorhina portentosa subjected to prolonged intoxication (7 weeks) with Cd and/or Se to investigate whether sublethal dose of cadmium (0.4 μmol, g-1 dry weight feed) would influence the detoxifying capabilities of the omnivorous insect, and whether selenium (0.01 μmol, g-1 dry weight feed) given concomitantly would act as Cd antagonist. Activities of CarE have been measured in crude homogenate, postmitochondrial supernatant and the pellet obtained from midgut, Malpighian tubules and fat body, using α-naphthyl acetate (αNA) and ρ-nitrophenyl acetate (ρNPA) as substrates. The highest rate of αNA hydrolysis occurred in midgut, whereas that of ρNPA in both the gut and Malpighian tubules. CarE activities in the Cd-group have been the highest in all tissues examined and for both substrates. A simultaneous administration of Cd and Se decreased the effects of Cd alone, probably as a result of partial elimination of the biologically active Cd pool. Selenium alone caused only minor effects when compared with cadmium. These results suggest, that disturbances of CarE activities depend on the Cd concentration in feed, and hence in tissues, as well as on possible interactions of the elements. © Springer-Verlag 1996.},
note = {4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Activities of non-specific carboxylesterases (CarE) have been studied in adult males of hissing cockroach Gromphadorhina portentosa subjected to prolonged intoxication (7 weeks) with Cd and/or Se to investigate whether sublethal dose of cadmium (0.4 μmol, g-1 dry weight feed) would influence the detoxifying capabilities of the omnivorous insect, and whether selenium (0.01 μmol, g-1 dry weight feed) given concomitantly would act as Cd antagonist. Activities of CarE have been measured in crude homogenate, postmitochondrial supernatant and the pellet obtained from midgut, Malpighian tubules and fat body, using α-naphthyl acetate (αNA) and ρ-nitrophenyl acetate (ρNPA) as substrates. The highest rate of αNA hydrolysis occurred in midgut, whereas that of ρNPA in both the gut and Malpighian tubules. CarE activities in the Cd-group have been the highest in all tissues examined and for both substrates. A simultaneous administration of Cd and Se decreased the effects of Cd alone, probably as a result of partial elimination of the biologically active Cd pool. Selenium alone caused only minor effects when compared with cadmium. These results suggest, that disturbances of CarE activities depend on the Cd concentration in feed, and hence in tissues, as well as on possible interactions of the elements. © Springer-Verlag 1996.