@article{2-s2.0-85163173309,

title = {Spatial consistency of cell growth direction during organ morphogenesis requires CELLULOSE SYNTHASE INTERACTIVE1},

author = { C. Mollier and J. Skrzydeł and D. Borowska-Wykręt and M. Majda and V. Bayle and V. Battu and J.C. Totozafy and M. Dulski and A. Fruleux and R. Wrzalik and G. Mouille and R.S. Smith and F. Monéger and D. Kwiatkowska and A. Boudaoud},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85163173309&doi=10.1016%2fj.celrep.2023.112689&partnerID=40&md5=ee793d110a083401054a5605aa7e1843},

doi = {10.1016/j.celrep.2023.112689},

issn = {22111247},

year  = {2023},

date = {2023-01-01},

journal = {Cell Reports},

volume = {42},

number = {7},

publisher = {Elsevier B.V.},

abstract = {Extracellular matrices contain fibril-like polymers often organized in parallel arrays. Although their role in morphogenesis has been long recognized, it remains unclear how the subcellular control of fibril synthesis translates into organ shape. We address this question using the Arabidopsis sepal as a model organ. In plants, cell growth is restrained by the cell wall (extracellular matrix). Cellulose microfibrils are the main load-bearing wall component, thought to channel growth perpendicularly to their main orientation. Given the key function of CELLULOSE SYNTHASE INTERACTIVE1 (CSI1) in guidance of cellulose synthesis, we investigate the role of CSI1 in sepal morphogenesis. We observe that sepals from csi1 mutants are shorter, although their newest cellulose microfibrils are more aligned compared to wild-type. Surprisingly, cell growth anisotropy is similar in csi1 and wild-type plants. We resolve this apparent paradox by showing that CSI1 is required for spatial consistency of growth direction across the sepal. © 2023 The Author(s)},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85104318817,

title = {Structure, assembly and function of cuticle from mechanical perspective with special focus on perianth},

author = { J. Skrzydeł and D. Borowska-Wykręt and D. Kwiatkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104318817&doi=10.3390%2fijms22084160&partnerID=40&md5=452697aa93e59e615f05a841ec65a2cb},

doi = {10.3390/ijms22084160},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {8},

publisher = {MDPI AG},

abstract = {This review is devoted to the structure, assembly and function of cuticle. The topics are discussed from the mechanical perspective and whenever the data are available a special attention is paid to the cuticle of perianth organs, i.e., sepals, petals or tepals. The cuticle covering these organs is special in both its structure and function and some of these peculiarities are related to the cuticle mechanics. In particular, strengthening of the perianth surface is often provided by a folded cuticle that functionally resembles profiled plates, while on the surface of the petal epidermis of some plants, the cuticle is the only integral continuous layer. The perianth cuticle is distinguished also by those aspects of its mechanics and development that need further studies. In particular, more investigations are needed to explain the formation and maintenance of cuticle folding, which is typical for the perianth epidermis, and also to elucidate the mechanical properties and behavior of the perianth cuticle in situ. Gaps in our knowledge are partly due to technical problems caused by very small thicknesses of the perianth cuticle but modern tools may help to overcome these obstacles. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85077615599,

title = {Deformation of a cell monolayer due to osmotic treatment: A case study of onion scale epidermis},

author = { S. Natonik-Białoń and D. Borowska-Wykręt and G. Mosca and M. Grelowski and R. Wrzalik and R.S. Smith and D. Kwiatkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077615599&doi=10.1139%2fcjb-2019-0027&partnerID=40&md5=a1843a05a469891e4ea23debf30b24c9},

doi = {10.1139/cjb-2019-0027},

issn = {19162804},

year  = {2020},

date = {2020-01-01},

journal = {Botany},

volume = {98},

number = {1},

pages = {21-36},

publisher = {Canadian Science Publishing},

abstract = {We performed a combination of experiments and mechanical simulations to assess the importance of cell geometry and wall structure in tissue and cell mechanics. Osmotic treatments combined with live imaging were used to quantify deformations at the tissue, cellular, and subcellular levels. We used the adaxial epidermis of onion scale as a model system. We found that the osmotically induced surface strain in onion is small because outer periclinal walls are thick and stiff, requiring bending stiffness to be considered in our mechanical models. As expected, the mechanical behaviors of the tissue and its component cells are related. Upon changes in internal pressure, cells embedded in the tissue undergo deformation that is different from isolated cells, while the tissue undergoes a somewhat counterintuitive deformation, e.g., shrinking upon pressurization, that depends on cell geometry. At the subcellular level, the amount of deformation and its anisotropy vary within the walls of individual cells, and are affected by the cell shape and vicinity of three-way wall junctions. When the turgor pressure is lost, the protoplast-facing wall surface wrinkles due to buckling, with the pattern of wrinkles depending on the strain anisotropy and the local wall geometry. © 2020, Canadian Science Publishing. All rights reserved.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85066835305,

title = {Erratum: Growing cell walls show a gradient of elastic strain across their layers (Journal of Experimental Botany Advance (2018) DOI: 10.1093/jxb/ery237)},

author = { M. Lipowczan and D. Borowska-Wykręt and S. Natonik-Białoń and D. Kwiatkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066835305&doi=10.1093%2fjxb%2fery280&partnerID=40&md5=cb20bdeb54380500da1f0b32475d2cfd},

doi = {10.1093/jxb/ery280},

issn = {00220957},

year  = {2018},

date = {2018-01-01},

journal = {Journal of Experimental Botany},

volume = {69},

number = {20},

pages = {4987-},

publisher = {Oxford University Press},

abstract = {Journal of Experimental Botany, Advance Access publication 26 June 2018, doi:10.1093/jxb/ery237 The original online version of this article contained errors in the typesetting of an equation, listed under 'Computation of minimum energy configurations of plates'. This equation, concerning the inward modulus (E) gradient described in part (ii), has now been corrected to read "(E1 > E2 > E3)". The publisher would like to apologise for this error. © 2018 Oxford University Press. All rights reserved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85055429118,

title = {Growing cell walls show a gradient of elastic strain across their layers},

author = { M. Lipowczan and D. Borowska-Wykręt and S. Natonik-Białoń and D. Kwiatkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85055429118&doi=10.1093%2fjxb%2fery237&partnerID=40&md5=74f7e7ffc91b4a79053ee8c5db2d4437},

doi = {10.1093/jxb/ery237},

issn = {00220957},

year  = {2018},

date = {2018-01-01},

journal = {Journal of Experimental Botany},

volume = {69},

number = {18},

pages = {4349-4362},

publisher = {Oxford University Press},

abstract = {The relatively thick primary walls of epidermal and collenchyma cells often form waviness on the surface that faces the protoplast when they are released from the tensile in-plane stress that operates in situ. This waviness is a manifestation of buckling that results from the heterogeneity of the elastic strain across the wall. In this study, this heterogeneity was confirmed by the spontaneous bending of isolated wall fragments that were initially flat. We combined the empirical data on the formation of waviness in growing cell walls with computations of the buckled wall shapes. We chose cylindrical-shaped organs with a high degree of longitudinal tissue stress because in such organs the surface deformation that accompanies the removal of the stress is strongly anisotropic and leads to the formation of waviness in which wrinkles on the inner wall surface are always transverse to the organ axis. The computations showed that the strain heterogeneity results from individual or overlaid gradients of pre-stress and stiffness across the wall. The computed wall shapes depend on the assumed wall thickness and mechanical gradients. Thus, a quantitative analysis of the wall waviness that forms after stress removal can be used to assess the mechanical heterogeneity of the cell wall. © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021145203,

title = {Gradient of structural traits drives hygroscopic movements of scarious bracts surrounding Helichrysum bracteatum capitulum},

author = { D. Borowska-Wykręt and A. Rypień and M. Dulski and M. Grelowski and R. Wrzalik and D. Kwiatkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021145203&doi=10.1093%2faob%2fmcx015&partnerID=40&md5=9a30620033957c30e3846802aa024e3d},

doi = {10.1093/aob/mcx015},

issn = {03057364},

year  = {2017},

date = {2017-01-01},

journal = {Annals of Botany},

volume = {119},

number = {8},

pages = {1365-1383},

publisher = {Oxford University Press},

abstract = {Background and Aims The capitulum of Helichrysum bracteatum is surrounded by scarious involucral bracts that perform hygroscopic movements leading to bract bending toward or away from the capitulum, depending on cell wall water status. The present investigation aimed at explaining the mechanism of these movements. Methods Surface strain and bract shape changes accompanying the movements were quantified using the replica method. Dissection experiments were used to assess the contribution of different tissues in bract deformation. Cell wall structure and composition were examined with the aid of light and electron microscopy as well as confocal Raman spectroscopy. Key Results At the bract hinge (organ actuator) longitudinal strains at opposite surfaces differ profoundly. This results in changes of hinge curvature that drive passive displacement of distal bract portions. The distal portions in turn undergo nearly uniform strain on both surfaces and also minute shape changes. The hinge is built of sclerenchyma-like abaxial tissue, parenchyma and adaxial epidermis with thickened outer walls. Cell wall composition is rather uniform but tissue fraction occupied by cell walls, cell wall thickness, compactness and cellulose microfibril orientation change gradually from abaxial to adaxial hinge surface. Dissection experiments show that the presence of part of the hinge tissues is enough for movements. Conclusions Differential strain at the hinge is due to adaxial-abaxial gradient in structural traits of hinge tissues and cell walls. Thus, the bract hinge of H. bracteatum is a structure comprising gradually changing tissues, from highly resisting to highly active, rather than a bi-layered structure with distinct active and resistance parts, often ascribed for hygroscopically moving organs. © The Author 2017. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84927145226,

title = {The CUP-SHAPED COTYLEDON2 and 3 genes have a post-meristematic effect on Arabidopsis thaliana phyllotaxis},

author = { A. Burian and M. Raczyńska-Szajgin and D. Borowska-Wykręt and A. Piatek and M. Aida and D. Kwiatkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84927145226&doi=10.1093%2faob%2fmcv013&partnerID=40&md5=1c4e3943f718abdd6aed21ddfe1422a9},

doi = {10.1093/aob/mcv013},

issn = {03057364},

year  = {2015},

date = {2015-01-01},

journal = {Annals of Botany},

volume = {115},

number = {5},

pages = {807-820},

publisher = {Oxford University Press},

abstract = {Background and Aims: The arrangement of flowers in inflorescence shoots of Arabidopsis thaliana represents a regular spiral Fibonacci phyllotaxis. However, in the cuc2 cuc3 double mutant, flower pedicels are fused to the inflorescence stem, and phyllotaxis is aberrant in the mature shoot regions. This study examined the causes of this altered development, and in particular whether the mutant phenotype is a consequence of defects at the shoot apex, or whether post-meristematic events are involved. Methods: The distribution of flower pedicels and vascular traces was examined in cross-sections of mature shoots; sequential replicas were used to investigate the phyllotaxis and geometry of shoot apices, and growth of the young stem surface. The expression pattern of CUC3 was analysed by examining its promoter activity. Key Results: Phyllotaxis irregularity in the cuc2 cuc3 double mutant arises during the post-meristematic phase of shoot development. In particular, growth and cell divisions in nodes of the elongating stem are not restricted in the mutant, resulting in pedicel-stem fusion. On the other hand, phyllotaxis in the mutant shoot apex is nearly as regular as that of the wild type. Vascular phyllotaxis, generated almost simultaneously with the phyllotaxis at the apex, is also much more regular than pedicel phyllotaxis. The most apparent phenotype of the mutant apices is a higher number of contact parastichies. This phenotype is associated with increased meristem size, decreased angular width of primordia and a shorter plastochron. In addition, the appearance of a sharp and deep crease, a characteristic shape of the adaxial primordium boundary, is slightly delayed and reduced in the mutant shoot apices. Conclusions: The cuc2 cuc3 double mutant displays irregular phyllotaxis in the mature shoot but not in the shoot apex, thus showing a post-meristematic effect of the mutations on phyllotaxis. The main cause of this effect is the formation of pedicel-stem fusions, leading to an alteration of the axial positioning of flowers. Phyllotaxis based on the position of vascular flower traces suggests an additional mechanism of post-meristematic phyllotaxis alteration. Higher density of flower primordia may be involved in the post-meristematic effect on phyllotaxis, whereas delayed crease formation may be involved in the fusion phenotype. Promoter activity of CUC3 is consistent with its post-meristematic role in phyllotaxis. © The Author 2015. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84893302816,

title = {FibrilTool, an ImageJ plug-in to quantify fibrillar structures in raw microscopy images},

author = { A. Boudaoud and A. Burian and D. Borowska-Wykręt and M. Uyttewaal and R. Wrzalik and D. Kwiatkowska and O. Hamant},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84893302816&doi=10.1038%2fnprot.2014.024&partnerID=40&md5=31d61c13cf41d7b9c2edf3ac85598e3e},

doi = {10.1038/nprot.2014.024},

issn = {17542189},

year  = {2014},

date = {2014-01-01},

journal = {Nature Protocols},

volume = {9},

number = {2},

pages = {457-463},

publisher = {Nature Publishing Group},

abstract = {Cell biology heavily relies on the behavior of fibrillar structures, such as the cytoskeleton, yet the analysis of their behavior in tissues often remains qualitative. Image analysis tools have been developed to quantify this behavior, but they often involve an image pre-processing stage that may bias the output and/or they require specific software. Here we describe FibrilTool, an ImageJ plug-in based on the concept of nematic tensor, which can provide a quantitative description of the anisotropy of fiber arrays and their average orientation in cells, directly from raw images obtained by any form of microscopy. FibrilTool has been validated on microtubules, actin and cellulose microfibrils, but it may also help analyze other fibrillar structures, such as collagen, or the texture of various materials. The tool is ImageJ-based, and it is therefore freely accessible to the scientific community and does not require specific computational setup. The tool provides the average orientation and anisotropy of fiber arrays in a given region of interest (ROI) in a few seconds. © 2014 Nature America, Inc. All rights reserved.},

note = {318},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84881023371,

title = {Defects in leaf epidermis of Arabidopsis thaliana plants with CDKA;1 activity reduced in the shoot apical meristem},

author = { D. Borowska-Wykręt and J. Elsner and L. De Veylder and D. Kwiatkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881023371&doi=10.1007%2fs00709-012-0472-9&partnerID=40&md5=a1c573c5e5706393384cf55385b3faa8},

doi = {10.1007/s00709-012-0472-9},

issn = {0033183X},

year  = {2013},

date = {2013-01-01},

journal = {Protoplasma},

volume = {250},

number = {4},

pages = {955-961},

abstract = {In Arabidopsis thaliana, like in other dicots, the shoot epidermis originates from protodermis, the outermost cell layer of shoot apical meristem. We examined leaf epidermis in transgenic A. thaliana plants in which CDKA;1.N146, a negative dominant allele of A-type cyclin-dependent kinase, was expressed from the SHOOTMERISTEMLESS promoter, i.e., in the shoot apical meristem. Using cleared whole mount preparations of expanding leaves and sequential in vivo replicas of expanding leaf surface, we show that dominant-negative CDKA;1 expression results in defects in epidermis continuity: loss of individual cells and occurrence of gaps between anticlinal walls of neighboring pavement cells. Another striking feature is ingrowth-like invaginations of anticlinal cell walls of pavement cells. Their formation is related to various processes: expansion of cells surrounding the sites of cell loss, defected cytokinesis, and presumably also, the actual ingrowth of an anticlinal cell wall. The mutant exhibits also increased variation in cell size and locally reduced waviness of anticlinal walls of pavement cells. These unusual features of leaf epidermis phenotype may shed a new light on our knowledge on morphogenesis of jigsaw puzzle-shaped pavement cells and on the CDKA;1 role in regulation of plant development via influence on cytoskeleton and plant cell wall. © 2012 The Author(s).},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-14944350419,

title = {Buckling of inner cell wall layers after manipulations to reduce tensile stress: Observations and interpretations for stress transmission},

author = { Z. Hejnowicz and D. Borowska-Wykręt},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-14944350419&doi=10.1007%2fs00425-004-1353-z&partnerID=40&md5=6886b382cc20bea93e2082a15a9e9525},

doi = {10.1007/s00425-004-1353-z},

issn = {00320935},

year  = {2005},

date = {2005-01-01},

journal = {Planta},

volume = {220},

number = {3},

pages = {465-473},

publisher = {Springer Verlag},

abstract = {The inner layer of the cell wall in tissues that are under tensile stress in situ, e.g. epidermis and collenchyma of etiolated sunflower hypocotyls, shows a pattern of transverse folds when the tissues are detached and plasmolysed. This can be observed by Nomarski imaging of inner surfaces of the outer cell walls and electron microscopy of longitudinal sections after peeling the epidermis and bathing it in plasmolysing solutions. The folds are apparently caused by buckling of the inner layer due to the longitudinal compressive force exerted on this layer by the outer wall layer, when it shrinks after the removal of the longitudinal tensile stresses. In these stresses, two components can be distinguished: the tissue stress, disappearing on peeling, and that caused directly by turgor pressure, disappearing in hyperosmotic solution. Investigation of the buckling indicates that the outer layer of the cell wall transmits in situ most of the longitudinal tensile stress in the wall. The common concept that the inner layer of the wall is the region bearing most stress and therefore regulating growth can still be valid with respect to the transverse stress component. © Springer-Verlag 2004.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}