@article{2-s2.0-85152731846,

title = {Ecotoxicological biotests as tools for continuous monitoring of water quality in dam reservoir [Biotesty ekotoksykologiczne jako narzędzie do ciągłego monitoringu jakości wody w zbiornikach zaporowych]},

author = { P. Łaszczyca and M. Nakonieczny and M. Kostecki},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85152731846&doi=10.24425%2faep.2023.144734&partnerID=40&md5=06784cd914cf7e1dce946a219d9a27de},

doi = {10.24425/aep.2023.144734},

issn = {20834772},

year  = {2023},

date = {2023-01-01},

journal = {Archives of Environmental Protection},

volume = {49},

number = {1},

pages = {25-38},

publisher = {Polska Akademia Nauk},

abstract = {Ecotoxicological biotests were applied in order to evaluate their suitability as early warning systems in the continuous monitoring of lowland shallow dam reservoirs located in Central Europe. The following biotests were used: Daphtoxkit F™magna, Algaltoxkit F™, Ostracodtoxkit F, Phytotoxkit and MARA Test. The experiment was conducted from July 2010 to December 2012 in Goczalkowice Reservoir (the Vistula River; Poland), serving as a model. For the analysis, 41 out of 52 measured water indices were used to assess its toxicity to living organisms. The results of biotests were correlated with 41 hydrochemical indices of water quality. The pattern of relationships among the result of biotest and hydrochemical indices as well as Factor Analysis (FA) and Primary Component Analysis (PCA) revealed that: i) signs of ecotoxicity detected with biotests were associated with either low flow periods or spring surface runoff of water; ii) single events of increased ecotoxicity in the depression areas behind saddle dam pump stations appeared after high flow periods; iii) elevated toxicity was accompanied by high concentrations of dissolved and suspended substances; iv) FA and PCA demonstrated correlations among the results of biotests and damming parameters, water conductivity, alkali and transitory metal metals (Ca, Fe, Cu, Zn), and several forms of nitrogen phosphorous and carbon compounds concentration. The relationships suggest that batteries of biotests may serve as a cost-effective tool for continuous monitoring of water quality in dam reservoirs and can detect effects of extreme hydrologic events, local toxic discharges, and signs of the trophic status of the reservoirs. © 2023. The Author(s).},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85122652069,

title = {Cydalima perspectalis in Poland—8 Years of Invasion against the Background of Three Other Invasive Species},

author = { P.K. Bereś and P. Ziętara and M. Nakonieczny and Ł. Kontowski and M. Grzbiela and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122652069&doi=10.3390%2fd14010022&partnerID=40&md5=15b64fb470791391107c78cb3228b29a},

doi = {10.3390/d14010022},

issn = {14242818},

year  = {2022},

date = {2022-01-01},

journal = {Diversity},

volume = {14},

number = {1},

publisher = {MDPI},

abstract = {The box tree moth (Cydalima perspectalis) origins from East Asia. In Europe, it was recorded for the first time in 2007, and in Poland in 2012. By the end of 2020, it was found all over Poland. There are no published data on the range of C. perspectalis occurrence in Poland because it is not a quarantine pest in the European Union and is not subject to official monitoring. Data collected in 2018–2020 via a website dedicated to monitoring, for the first time, illustrate the current range and its largest concentrations in southern and central Poland. The monitoring confirmed that the main directions of the invasion are related to the main communication routes of Poland (south-north) and are of a long-distance character. The dispersal pattern corresponds to the model developed for Cameraria ohridella: a stratified dispersal model that considers long-distance road/rail transport. The second important factor contributing to the invasion of C. perspectalis are large human communities enabling rapid local dispersion (a diffusion model). Comparing its invasion with the monitoring data from 2007–2013 of two other invasive pests of Poland: Ostrinia nubilalis and Diabrotica virgifera, shows that a diffusion model best describes the spatial spread of these pests only to uninhabited neighboring areas. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85104957867,

title = {Towards understanding Cameraria ohridella (Lepidoptera: Gracillariidae) development: effects of microhabitat variability in naturally growing horse-chestnut tree canopy},

author = { P. Łaszczyca and M. Nakonieczny and A. Kędziorski and A. Babczyńska and M. Wiesner},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104957867&doi=10.1007%2fs00484-021-02119-8&partnerID=40&md5=2b01bb6369bde5c53f17613274c9d44c},

doi = {10.1007/s00484-021-02119-8},

issn = {00207128},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Biometeorology},

volume = {65},

number = {10},

pages = {1647-1658},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {Dwelling intensity of horse-chestnut miner (Cameraria ohridella) larvae in various leaves insolation and temperature was measured to determine whether this pest’s development follows a predictable pattern or depends more on local microenvironment conditions. Mines growing on leaves of mature host plants (Aesculus hippocastanum L.) in their natural conditions were photographed for two consecutive generations of the pest and in two separated vegetation periods. Apart from meteorological data obtained from the nearest station, the temperature of intact and mined parts of sun-exposed and shaded leaf blades was measured at various daytimes throughout the experiment. Obtained sets of digital data were analysed and combined to model mine area growth as a function of degree-days sum by adopting of Verhulst logistic equation. We showed the predictive potential of our model based on experimental data, and it may be useful in the scheduling of pest control measures in natural conditions. Our analyses also revealed that despite significant differences in microenvironment conditions depending on mines’ insolation, the horse-chestnut miner larvae could partially compensate for them and complete their development at similar endpoints expressed as the cumulative sum of degree-days. We conclude that computer-aided analysis of photographic documentation of leaf-miner larval growth followed by mathematical modelling offers a noninvasive, reliable, and inexpensive alternative for monitoring local leaf-miners populations. © 2021, The Author(s).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85095429320,

title = {Transmission of Pantoea ananatis, the causal agent of leaf spot disease of maize (Zea mays), by western corn rootworm (Diabrotica virgifera virgifera LeConte)},

author = { K. Krawczyk and J. Foryś and M. Nakonieczny and M. Tarnawska and P.K. Bereś},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85095429320&doi=10.1016%2fj.cropro.2020.105431&partnerID=40&md5=dff84c6a39823dd2dcd518488a06b411},

doi = {10.1016/j.cropro.2020.105431},

issn = {02612194},

year  = {2021},

date = {2021-01-01},

journal = {Crop Protection},

volume = {141},

publisher = {Elsevier Ltd},

abstract = {The aim of this study was to determine the role of Diabrotica virgifera virgifera (western corn rootworm; WCR) as a potential insect vector of the pathogenic bacterium Pantoea ananatis. The experiment included a greenhouse test to investigate if WCR was able to transfer the pathogenic bacteria from infected to healthy maize plants. Adult WCR specimens collected from maize fields near Rzeszów were used in the experiment. The plant materials were sweetcorn plants of the Waza variety. Pure cultures of previously verified P. ananatis strain M241 were the source of the inoculum. Insects caught under natural conditions were incubated in an isolator containing pathogen-negative plants. Randomly selected insects were then examined for the presence of bacterial pathogens of maize in their digestive tract. Pathogen-negative insects were used in the next stage of the experiment still carried out in isolators, in which the insects foraged on maize seedlings previously infected with P. ananatis. The control group consisted of healthy, uninfected insects and plants. After the incubation period, the presence of bacterial pathogens in the gastrointestinal tract of the WCR specimens was confirmed. Subsequent insects that acquired P. ananatis were bred on pathogen-negative maize plants. After incubation, the presence of pathogenic bacteria in the body of the examined WCR beetles was confirmed, and the presence of symptoms of the bacterial disease was noted on maize plants, fulfilling Koch's postulates and indicating that the WCR is a vector of P. ananatis on maize plants. This is the first report on the vectorization of P. ananatis by D. virgifera globally. © 2020 Elsevier Ltd},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85094658481,

title = {Set of stress biomarkers as a practical tool in the assessment of multistress effect using honeybees from urban and rural areas as a model organism: a pilot study},

author = { Ł. Nicewicz and A.W. Nicewicz and A. Kafel and M. Nakonieczny},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85094658481&doi=10.1007%2fs11356-020-11338-2&partnerID=40&md5=30743a0ae7152df57463bbe5cd24fb23},

doi = {10.1007/s11356-020-11338-2},

issn = {09441344},

year  = {2021},

date = {2021-01-01},

journal = {Environmental Science and Pollution Research},

volume = {28},

number = {8},

pages = {9084-9096},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {A decrease among honey bee populations (Apis mellifera) in the traditional apiaries has been observed in recent years. In light of this negative phenomenon, urban beekeeping seems to be an appropriate alternative solution for the bee population in reducing the toxic effects of a large number of pesticides that are commonly used in agricultural ecosystems. Despite the rapid development of urban beekeeping, there is little information regarding the different aspects of the defense effectiveness of bees from the urban and rural areas. The study was aimed to show whether honey bees from these two locations differ in the level of the valuable biomarkers of stress exposure helpful in establishing which bees, from urban or rural areas, are under greater environmental pressure. For this purpose, foragers from an urban rooftop apiary and a traditional rural apiary were collected. The chosen biomarkers were measured in various tissues of bees. The activity of glutathione S-transferase and acetylcholinesterase, the level of total antioxidant capacity, heat shock protein 70 (Hsp70), and defensin were selected for the analyses. In our opinion, the Hsp70 and defensin levels seemed to be important in the indication of urban multistress factors. The higher level of heat shock proteins and defensins in tissues/organs of bees from the urban apiary—in the gut (an increase; respectively; 92% and 7.3%) and fat body (an increase; respectively; 130% and 7.8%), known as targets of environmental toxins, pointed out the urban environment as highly stressful at both the individual and colony levels. In turn, high total antioxidant capacity was measured in the guts of honey bees from rural area (an increase 107%). Such a situation suggests a different mechanism of defense and specificity of rural and urban environmental stressors and also honey bees foraging activity. © 2020, The Author(s).},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85075341430,

title = {Esterase activity and heat shock protein levels in western corn rootworm beetles (Diabrotica virgifera virgifera LeConte, Coleoptera: Chrysomelidae) after pesticide exposure},

author = { M. Tarnawska and P.K. Bereś and S. Drzewiecki and J. Guzik and P. Migula and K.R. Brom and K. Brzozowska-Wojoczek and B. Doleżych and M. Nakonieczny},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075341430&doi=10.1080%2f09670874.2019.1687958&partnerID=40&md5=835701f0af70ec5cf8404c94b54dccec},

doi = {10.1080/09670874.2019.1687958},

issn = {09670874},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Pest Management},

volume = {67},

number = {2},

pages = {99-107},

publisher = {Taylor and Francis Ltd.},

abstract = {The aim of this study was to investigate how the exposure of the western corn rootworm beetle (WCR) to dimethoate might affect their ability to detoxify and Hsps response. The activity of the esterases: acetylcholinesterase (AChE) and carboxylesterase (CbE) and the level of Hsp90, Hsp70 and Hsp40 was assayed in adult individuals 1 h and 24 h after a topical pesticide application. A slight increase in the esterase activity 1 h after pesticide exposure was followed by a significant decrease in AChE after 24 h. The ELISA method revealed differences in the Hsps concentrations that were dependent on the protein family. Western Blotting data confirmed these results. The esterase activity and Hsps response are discussed. © 2019 Informa UK Limited, trading as Taylor & Francis Group.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84954403049,

title = {Population dynamics of Western corn rootworm beetles on different varieties of maize identified using pheromone and floral baited traps},

author = { P.K. Bereś and S. Drzewiecki and M. Nakonieczny and M. Tarnawska and J. Guzik and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84954403049&doi=10.1017%2fS0021859615000222&partnerID=40&md5=f84ebbbde7d3a78c9552c49f63a88a5c},

doi = {10.1017/S0021859615000222},

issn = {00218596},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Agricultural Science},

volume = {153},

number = {8},

pages = {1479-1490},

publisher = {Cambridge University Press},

abstract = {The present paper presents details of the population dynamics of Diabrotica v. virgifera LeConte beetles on six maize varieties of three different maturity classes, i.e. early (FAO 190), medium-early (FAO 250) and medium-late (FAO 280-300), grown in Central and Eastern Europe. The study was conducted in 2009/10 in the south-eastern part of Poland. The D. virgifera beetles were captured using two types of trap: pheromone (PTs) and floral-baited (FTs). Significant differences in beetle counts were found between: (i) the first and second year of the study (higher in 2009 than in 2010); (ii) the varieties of maize and their different growth stages; (iii) early and medium-late varieties of maize. Initially, depending on the availability of maize silk and pollen, the most numerous D. virgifera beetles were found on the early varieties followed by the medium-late varieties. The study also revealed a significant difference in the performance of the two types of trap used to monitor the number of adults, particularly during the initial period when arrival rates were high. Pheromone traps were more effective in catching D. virgifera beetles, especially during the flowering stage of maize. Floral-baited traps were most effective at the end of the active growth of maize, when the preferred food of D. virgifera was unavailable. © Cambridge University Press 2015.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85009892699,

title = {Glycolytic enzymes activity in the midgut of Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) adult and their seasonal changes},

author = { J. Guzik and M. Nakonieczny and M. Tarnawska and P.K. Bereś and S. Drzewiecki and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009892699&doi=10.1093%2fjisesa%2fiev036&partnerID=40&md5=c108792bd08349e23d6ec34a5bdce674},

doi = {10.1093/jisesa/iev036},

issn = {15362442},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Insect Science},

volume = {15},

number = {1},

publisher = {Library of the University of Arizona},

abstract = {The western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) is an important pest of maize. The diet of the D. virgifera imago is rich in starch and other polysaccharides present in cereals such as maize. Therefore, knowledge about enzymes involved in digestion of such specific food of this pest seems to be important. The paper shows, for the first time, the activities of main glycolytic enzymes in the midgut of D. virgifera imago: endoglycosidases (α-amylase; cellulase; chitinase; licheninase; laminarinase); exoglycosidases (α- and β-glucosidases; α- and β-galactosidases) and disaccharidases (maltase; isomaltase; sucrase; trehalase; lactase; and cellobiase). Activities of α-amylase, α-glucosidase, and maltase were the highest among assayed endoglycosidases, exoglycosidases, and disaccharidases, respectively. This indicates that in the midgut of D. virgifera imago a-amylase, α-glucosidase and maltase are important enzymes in starch hydrolysis and products of its digestion. These results lead to conclusion that inhibition of most active glycolytic enzymes of D. virgifera imago may be another promising method for chemical control of this pest of maize. © The Author 2015. Published by Oxford University Press on behalf of the Entomological Society of America.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84956691211,

title = {Expression of the Hsp40, Hsp70 and Hsp90 proteins in Colorado potato beetle (Leptinotarsa decemlineata Say) after the dimethoate treatment},

author = { K.R. Brom and B. Doleżych and M. Tarnawska and K. Brzozowska and M. Nakonieczny},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956691211&partnerID=40&md5=b0bec8c31a4ea850a312ef254dc2de33},

issn = {13020250},

year  = {2015},

date = {2015-01-01},

journal = {Journal of the Entomological Research Society},

volume = {17},

number = {2},

pages = {39-49},

publisher = {Gazi Entomological Research Society},

abstract = {Hsp40, Hsp70 and Hsp90 proteins exhibit increased level of expression in animals exposed to negative environmental conditions, including pesticides, but the measured effect depends on the class which particular Hsp is belonging to. In this study we examined Hsp level in the Colorado potato beetle exposed to pesticide dimethoate by using Western blotting and ELISA method. The highest level of expression was exhibited by Hsp90, while Hsp70 showed the lowest level of expression. There were no statistically significant differences in the expressions of Hsp40 and Hsp70 after the pesticide treatment. The highest concentration of Hsp90 was found in beetles 24 hours after the exposition to dimethoate, while the lowest was observed in insects 1 hour after the treatment. Hsps play a role in response to organophosphorous pesticide in Leptinotarsa decemlineata (Say; 1824), but there is not any simple correlation between Hsp levels and time of exposition. Hsp90 seems to be the most suitable protein to examine pesticide-provoked stress.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84924420592,

title = {Elemental distribution in reproductive and neural organs of the epilachna nylanderi (Coleoptera: Coccinellidae), a phytophage of nickel hyperaccumulator Berkheya coddii (Asterales: Asteraceae) by micro-PIXE},

author = { J. Mesjasz-Przybyłowicz and E. Orłowska and M. Augustyniak and M. Nakonieczny and M. Tarnawska and W.J. Przybyłowicz and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924420592&doi=10.1093%2fjisesa%2fieu014&partnerID=40&md5=b9fa04f75525193486d5820a32e309b4},

doi = {10.1093/jisesa/ieu014},

issn = {15362442},

year  = {2014},

date = {2014-01-01},

journal = {Journal of Insect Science},

volume = {14},

publisher = {Library of the University of Arizona},

abstract = {The phenomenon of metal hyperaccumulation by plants is often explained by a pathogen or herbivore defense hypothesis. However, some insects feeding on metal hyperaccumulating plants are adapted to the high level of metals in plant tissues. Former studies on species that feed on the leaves of Berkheya coddii Roessler 1958 (Asteraceae), a nickel-hyperaccumulating plant, demonstrated several protective mechanisms involved in internal distribution, immobilization, and elimination of Ni from the midgut and Malpighian tubules. These species are mainly coleopterans, including the lady beetle, Epilachna nylanderi (Mulsant 1850) (Coleoptera: Coccinellidae), collected from the ultramafic ecosystem near Barberton in South Africa. By performing particle-induced X-ray emission microanalysis elemental microanalysis (PIXE), this study examined whether Ni may be harmful to internal body systems that decide on insect reactivity (central nervous system [CNS]), their reproduction, and the relationships between Ni and other micronutrients. Data on elemental distribution of nine selected elements in target organs of E. nylanderi were compared with the existing data for other insect species adapted to the excess of metals. Micro-PIXE maps of seven regions of the CNS showed Ni mainly in the neural connectives, while cerebral ganglia were better protected. Concentrations of other bivalent metals were lower than those of Ni. Testis, compared with other reproductive organs, showed low amounts of Ni. Zn was effectively regulated at physiological dietary levels. In insects exposed to excess dietary Zn, it was also accumulated in the reproductive organs. Comparison of E. nylanderii with other insects that ingest hyperaccumulating plants, especially chrysomelid Chrysolina clathrata (Clark) (Coleoptera: Chrysomelidae), showed lower protection of the CNS and reproductive organs. © The Author 2014. Published by Oxford University Press on behalf of the Entomological Society of America.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84901241774,

title = {Parnassius apollo last-instar larvae development prediction by analysis of weather condition as a tool in the species' conservation},

author = { B. Łozowski and A. Kędziorski and M. Nakonieczny and P. Łaszczyca},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901241774&doi=10.1016%2fj.crvi.2014.03.004&partnerID=40&md5=c15d9fa248f59d5b88e594a7a6d4ae43},

doi = {10.1016/j.crvi.2014.03.004},

issn = {16310691},

year  = {2014},

date = {2014-01-01},

journal = {Comptes Rendus - Biologies},

volume = {337},

number = {5},

pages = {325-331},

publisher = {Elsevier Masson SAS},

abstract = {Parnassius apollo (Lepidoptera: Papilionidae) has already disappeared or is under threat of extinction in many of its former habitats. It has been documented that weather conditions - anomalies in particular - contributed to this process. In this study, we combined developmental data obtained previously for the last-instar Apollo larvae (collected in 1996; 1997; and 2003) with corresponding meteorological data to assess the effects of ambient temperature and rainfall episodes on the duration and the completion of the instar. For comparing the temperature effect, we applied the degree-day concept. We found significant positive correlation between the number of rainy days during the instar development (x) and its duration time (y): y = 8.293 + 0.936x (± 2.813) (r = 0.662; P < 10-7). Logarithmic transformation of the growth curves of the last-instar Apollo larvae revealed that there was no difference in growth among females; however, there was slower growth of males in 2003 in comparison to 1996. Growth (y) of female Apollo larvae as a function of instar duration (x) can by described by one common equation, irrespectively of the year: y = 317.6 + 502.3 lnx (± 263.3) (r = 0.82; P < 10 -4). © 2014 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84873195271,

title = {Digestive enzymes activity in subsequent generations of Cameraria ohridella larvae harvested from horse chestnut trees after treatment with imidacloprid},

author = { D. Stygar and K. Michalczyk and B. Doleżych and M. Nakonieczny and P. Migula and M. Zaak and T. Sawczyn and I. Karcz-Socha and M. Kukla and K. Żwirska-Korczala and R.J. Buldak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84873195271&doi=10.1016%2fj.pestbp.2012.10.003&partnerID=40&md5=f5862fb38acc7c516e5149e7081d993d},

doi = {10.1016/j.pestbp.2012.10.003},

issn = {00483575},

year  = {2013},

date = {2013-01-01},

journal = {Pesticide Biochemistry and Physiology},

volume = {105},

number = {1},

pages = {5-12},

abstract = {In the present study we describe the effect of chloronicotinoid pesticide (imidacloprid) on the digestive enzymes activity of the Cameraria ohridella larvae after lasting 1. year sublethal exposure to imidacloprid pesticide. Caterpillars - L4 stage (fourth instar; hyperphagic tissue-feeding phase) - were collected from chemically protected white horse chestnut trees 1. year after imidacloprid treatment, and compared with caterpillars collected from non-treated trees in a previous study. Enzymes activity of α-amylase, disaccharidases, glycosidases and proteases was assayed. The presence of pesticide in ingested food changed the digestive enzymes profile of caterpillars. The analysis of correlations between different digestive enzymes showed many significant correlations (. P<. 0.05) among glycolytic activities like β-glucosidase and α-galactosidase activities. Statistically significant correlations for proteolytic activity were found between trypsin and chymotrypsin activity and aminopeptidase activity that occurred only in the 1st generation. PCA distinguished five primary components with eigenvalues higher than 1, from which the first two explain almost 59% of analyzed results. Surprisingly, in the pesticide treated groups significantly higher activities of sucrase and lactase in relation to control were found. In general, glycosidase (α-glucosidase; β-glucosidase and β-galactosidase) activities showed a similar pattern of activity in different generations. These results contrast with those obtained with control larvae, where significant differences in activities of α-glucosidase, β-glucosidase and β-galactosidase may result from the different quantity and quality food intake by subsequent generations of larvae. No inter-generation differences in total proteolytic activity were observed in treated larvae. The absolute value of total proteolytic activity was higher than that in the control group. The pesticide present in the vascular system of the horse chestnut tree significantly affected some of the digestive enzymes activities and - in consequence - also interrelationships between enzymes, what may affect the food digestion. © 2012 Elsevier Inc.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84861348718,

title = {Occurrence of Sarmentosin and Other Hydroxynitrile Glucosides in Parnassius (Papilionidae) Butterflies and Their Food Plants},

author = { N. Bjarnholt and M. Nakonieczny and A. Kędziorski and D.M. Debinski and S.F. Matter and C.E. Olsen and M. Zagrobelny},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84861348718&doi=10.1007%2fs10886-012-0114-x&partnerID=40&md5=a4d52b35eb6469e65a194910b73afb9e},

doi = {10.1007/s10886-012-0114-x},

issn = {00980331},

year  = {2012},

date = {2012-01-01},

journal = {Journal of Chemical Ecology},

volume = {38},

number = {5},

pages = {525-537},

abstract = {Sequestration of plant secondary metabolites is a widespread phenomenon among aposematic insects. Sarmentosin is an unsaturated γ-hydroxynitrile glucoside known from plants and some Lepidoptera. It is structurally and biosynthetically closely related to cyanogenic glucosides, which are commonly sequestered from food plants and/or de novo synthesized by lepidopteran species. Sarmentosin was found previously in Parnassius (Papilionidae) butterflies, but it was not known how the occurrence was related to food plants or whether Parnassius species could biosynthesize the compound. Here, we report on the occurrence of sarmentosin and related compounds in four different Parnassius species belonging to two different clades, as well as their known and suspected food plants. There were dramatic differences between the two clades, with P. apollo and P. smintheus from the Apollo group containing high amounts of sarmentosin, and P. clodius and P. mnemosyne from the Mnemosyne group containing low or no detectable amounts. This was reflected in the larval food plants; P. apollo and P. smintheus larvae feed on Sedum species (Crassulaceae), which all contained considerable amounts of sarmentosin, while the known food plants of the two other species, Dicentra and Corydalis (Fumariaceae), had no detectable levels of sarmentosin. All insects and plants containing sarmentosin also contained other biosynthetically related hydroxynitrile glucosides in patterns previously reported for plants, but not for insects. Not all findings could be explained by sequestration alone and we therefore hypothesize that Parnassius species are able to de novo synthesize sarmentosin. © 2012 Springer Science+Business Media, LLC.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84857440067,

title = {The localisation of HSP70 and oxidative stress indices in heads of Spodoptera exigua larvae in a cadmium-exposed population},

author = { A. Kafel and A. Nowak and J. Bembenek and J. Szczygieł and M. Nakonieczny and R. Świergosz-Kowalewska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84857440067&doi=10.1016%2fj.ecoenv.2011.10.024&partnerID=40&md5=3bc04f13cdef21578e60fc5462187bf1},

doi = {10.1016/j.ecoenv.2011.10.024},

issn = {01476513},

year  = {2012},

date = {2012-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {78},

pages = {22-27},

abstract = {The effects of cadmium toxicity may vary between animals with different history of metal exposure. The aim of our study was to examine HSP70, protein carbonyl levels, catalase activity and total antioxidant capacity in the heads of Spodoptera exigua (Hübner) larvae originated from undergoing 1- and 44-generational cadmium treatment and in control (those that were not exposed to cadmium). We also measured the cadmium concentration and DNA damage level in the larvae.We observed higher level of heat shock proteins (HSPs) in the heads of larvae derived from multi-generational metal treatment than in the heads of those from one-generational treatment (derived from the control rearing). Analysis of HSP localisation in the larval brain suggests that these changes could be important for protecting the neural function of larval mushroom bodies for animals selected during multigenerational metal exposure. Animals from one-generational treatment had, in turn, higher total antioxidant capacity than animals from multigenerational treatment. Anyway, animals from one- and 44-generational metal treatments did not differ in metal accumulation in the heads and the whole larval bodies, catalase activity or DNA damage level. All these measurements were higher than for control larvae and cadmium accumulation in the heads was much lower than in the whole bodies. © 2011 .},

note = {32},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79955156773,

title = {Micro-PIXE studies of Ni-elimination strategies in representatives of two families of beetles feeding on Ni-hyperaccumulating plant Berkheya coddii},

author = { P. Migula and W.J. Przybyłowicz and M. Nakonieczny and M. Augustyniak and M. Tarnawska and J. Mesjasz-Przybyłowicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955156773&doi=10.1002%2fxrs.1310&partnerID=40&md5=61672de73a5cbfaa8e978a3de14d6f8f},

doi = {10.1002/xrs.1310},

issn = {00498246},

year  = {2011},

date = {2011-01-01},

journal = {X-Ray Spectrometry},

volume = {40},

number = {3},

pages = {194-197},

abstract = {Plants may use the ability to hyperaccumulate toxic metals in their defence against herbivores. Insects can counteract and feed on toxic plants. Among grazers of Ni-hyperaccumulating Berkheya coddii, some beetles tolerate Ni (Chrysolina clathrata; former name Chrysolina pardalina; Epilachna nylanderi). The adaptive mechanisms against Ni toxicity were studied in these species using micro-PIXE quantitative elemental mapping and by microscopy techniques. Distinct differences were found in mechanisms used for Ni-elimination in both species. Functional comparisons and quantification of data extracted from selected micro-areas in target organs responsible for metal circulation (digestive tract and Malpighian tubules) were made. Similar detoxification mechanism is used against metals through spheric granules, which in E. nylanderi are formed mainly in the midgut, while in C. clathrata in Malpighian tubules and midgut. Elemental maps showed positive correlations of Ni and Zn in Malpighian tubules of E. nylanderi without such effect in C. clathrata. Quantitative relations of K+ and Cl- indicate higher osmotic pressure increasing reabsorption of water necessary for Ni transportation to the intestine lumen and further rejection outside the body. © 2011 John Wiley & Sons, Ltd.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77958182524,

title = {Digestive enzymes activity in larvae of Cameraria ohridella (Lepidoptera: Gracillariidae)},

author = { D. Stygar and B. Doleżych and M. Nakonieczny and P. Migula and K. Michalczyk and M. Zaak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77958182524&doi=10.1016%2fj.crvi.2010.08.004&partnerID=40&md5=3ce9aa823be04fd864e8a54e802cb607},

doi = {10.1016/j.crvi.2010.08.004},

issn = {16310691},

year  = {2010},

date = {2010-01-01},

journal = {Comptes Rendus - Biologies},

volume = {333},

number = {10},

pages = {725-735},

abstract = {This article presents the activity of carbohydratases and proteases in the midgut of Cameraria ohridella larvae - an oligophagous pest whose preferred feeding is horse chestnuts leaves. Optimal media pH of the assayed enzymes were similar to those of other Lepidopterans. Relatively high amylase activity, as well as maltase and sucrase activities, indicates that starch and sucrose are the main digested saccharides. Trehalase activity was similar to that described in other Lepidopterans. Activities of glycosidases were significantly lower than those of disaccharidases what suggests that neither cellulose nor glycosides are important for C. ohridella. Trypsin is the main endoprotease of this pest. Like in other leaf-eaters carboxypeptidase activity was higher than that of aminopeptidase. The activity of the majority of examined enzymes increased in the following successive pest generations, which could be explained by the decreased nutritional value of older leaves. Probably this phenomenon in hydrolases activity in Cameraria is a nonspecific mechanism present at this stage of co-evolution of the horse chestnut and its pest. © 2010 Published by Elsevier Masson SAS on behalf of Académie des sciences.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-35548947013,

title = {Short-term effects of dimethoate on metabolic responses in Chrysolina pardalina (Chrysomelidae) feeding on Berkheya coddii (Asteraceae), a hyper-accumulator of nickel},

author = { M. Augustyniak and P. Migula and J. Mesjasz-Przybyłowicz and M. Tarnawska and M. Nakonieczny and A. Babczyńska and W.J. Przybyłowicz and M.G. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-35548947013&doi=10.1016%2fj.envpol.2007.01.018&partnerID=40&md5=144d40e423946ba636a4893b7c289520},

doi = {10.1016/j.envpol.2007.01.018},

issn = {02697491},

year  = {2007},

date = {2007-01-01},

journal = {Environmental Pollution},

volume = {150},

number = {2},

pages = {218-224},

abstract = {Berkheya coddii Roessler (Asteraceae) is a hyper-accumulator of nickel, which can be used in phytomining and phytoremediation. Chrysolina pardalina Fabricius (Chrysomelidae) is a phytophagous leaf beetle, which may be useful in controlling population levels of B. coddii after it has been introduced into a new habitat. The aim of this study was to investigate the response of C. pardalina to topical application of dimethoate. Data recorded included the activity of acetylcholinesterase (AChE), the concentration of glutathione (GSH), and the activity of selected enzymes connected with GSH metabolism. Assays were carried out several times during the first 24 h after exposure to dimethoate. At the dosages used in this study, dimethoate was not as toxic as expected. AChE activity was significantly decreased 14 and 24 h after application. GST activity was significantly decreased 24 h after application. GSTPx activity was significantly decreased 2, 14 and 24 h after application. GR activity was significantly increased 4 h after application. GSH concentration was significantly increased 24 h after application. Long-term exposure to high levels of nickel may have caused adaptive changes in the enzymes that enable C. pardalina to deal with other stressors, including organophosphate pesticides. © 2007 Elsevier Ltd. All rights reserved.},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33846888552,

title = {Midgut protease activities in monophagous larvae of Apollo butterfly, Parnassius apollo ssp. frankenbergeri},

author = { M. Nakonieczny and K. Michalczyk and A. Kędziorski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846888552&doi=10.1016%2fj.crvi.2006.12.002&partnerID=40&md5=07c8b860616c988e592952f39cfcd884},

doi = {10.1016/j.crvi.2006.12.002},

issn = {16310691},

year  = {2007},

date = {2007-01-01},

journal = {Comptes Rendus - Biologies},

volume = {330},

number = {2},

pages = {126-134},

abstract = {We assayed the relative activities of midgut proteolytic enzymes in individuals of the fourth (L4) and fifth (L5) instar of Apollo larvae, inhabiting Pieniny Mts (southern Poland). The comparisons between midgut tissue with glicocalyx (MT) and liquid midgut contents with peritrophic membrane (MC) were made. Optimal media pHs of the assayed proteolytic enzymes in P. apollo midgut samples were similar to those of other lepidopteran species. Endopeptidases, as well as carboxypeptidases, digested effectively in alkaline environment, while aminopeptidases were active in a broad pH range. Trypsin is probably the main endoprotease (correlation with caseinolytic activity in MC of L5 larvae: r = 0.606; p = 0.004); however, its activity was low as compared with that in other leaf-eating Lepidoptera. This suggests a minor role of trypsin and chymotrypsin in protein digestion in Apollo larvae, probably due to limited availability of the leaf proteins. Instead, due to very high carboxypeptidase A activity in midgut tissue, the larvae obtain exogenous amino acids either directly or from oligopeptides and glycoproteins. High and significant positive correlations between the enzyme activity and glucosidase as well as galactosidase activities strongly support this opinion. To cite this article: M. Nakonieczny et al., C. R. Biologies 330 (2007). © 2006 Académie des sciences.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33749254989,

title = {Midgut glycosidases activities in monophagous larvae of Apollo butterfly, Parnassius apollo ssp. frankenbergeri},

author = { M. Nakonieczny and K. Michalczyk and A. Kędziorski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749254989&doi=10.1016%2fj.crvi.2006.06.009&partnerID=40&md5=693bd6decf31c3de80afc9be2f2a0f49},

doi = {10.1016/j.crvi.2006.06.009},

issn = {16310691},

year  = {2006},

date = {2006-01-01},

journal = {Comptes Rendus - Biologies},

volume = {329},

number = {10},

pages = {765-774},

abstract = {Parnassius apollo (Lepidoptera; Papilionidae) declines on numerous localities all over Europe. Its local subspecies frankenbergeri, inhabiting the Pieniny Mts (southern Poland) and successfully recovered from extinction, is monophagous in larval stage. In natural conditions, it completes development on the orpine Sedum telephium ssp. maximum. Since proper quality and quantity of necessary nutritional compounds of the food plant ensure developmental success, the digestive processes in the insect midgut should reflect adaptation to a specific food source. The paper presents, for the first time, the activity of detected glycolytic enzymes in midgut tissue and liquid gut contents of the L4 and L5 instars of P. apollo larvae. α-Amylase plays the main role in utilization of carbohydrates, contrary to cellulase activity. Saccharase seems to be the main disaccharidase, and high activity of β-glycosidase enables hydrolysis of the plant glycosides. Trehalase activity was unexpectedly low and comparable to those of cellobiase and lactase. α-Amylolytic and other glycolytic activities indicate that larvae utilize starch and other carbohydrate compounds as energy sources. Possible use of some plant allelochemicals as energy sources by Apollo larvae is discussed. To cite this article: M. Nakonieczny et al., C. R. Biologies 329 (2006). © 2006 Académie des sciences.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-14844358518,

title = {Feeding preferences of the Apollo butterfly (Parnassius apollo ssp. frankenbergeri) larvae inhabiting the Pieniny Mts (southern Poland)},

author = { M. Nakonieczny and A. Kędziorski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-14844358518&doi=10.1016%2fj.crvi.2004.12.004&partnerID=40&md5=49685c75626b12af9e163010527fb4c7},

doi = {10.1016/j.crvi.2004.12.004},

issn = {16310691},

year  = {2005},

date = {2005-01-01},

journal = {Comptes Rendus - Biologies},

volume = {328},

number = {3},

pages = {235-242},

abstract = {Parnassius apollo (Lepidoptera; Papilionidae) is considered to be typical stenophagous species. Its European forms fall generally into 'telephiophagous' or 'albophagous' trophic groups. According to some authors, 'telephiophagous' P. apollo ssp. frankenbergeri Slaby, inhabiting the Pieniny Mts, has a rather broad spectrum of food-plants. We aimed at defining its feeding preferences for successful breeding in a semi-natural colony on more than one commercially-available Sedum species. Larval development (L5) and performance were assessed in three experimental groups fed on different plant species selected in a preliminary test. Apollo larvae appeared to be quite specific in the plant choice and developed poorly in the absence of Sedum telephium. Possible reasons of this are discussed. © 2004 Académie des sciences. Published by Elsevier SAS. All rights reserved.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-15444375342,

title = {Uptake of cadmium, lead, nickel and zinc from soil and water solutions by the nickel hyperaccumulator berkheya coddii},

author = { J. Mesjasz-Przybyłowicz and M. Nakonieczny and P. Migula and M. Augustyniak and M. Tarnawska and W.U. Reimold and C. Koeberl and W.J. Przybyłowicz and E. Głowacka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-15444375342&partnerID=40&md5=dc4ede8c4a0607a1f92bba5e4b45faf0},

issn = {00015296},

year  = {2004},

date = {2004-01-01},

journal = {Acta Biologica Cracoviensia Series Botanica},

volume = {46},

pages = {75-85},

abstract = {Berkheya coddii Roessler (Asteraceae), an endemic herbaceous and perennial nickel-hyperaccumulating plant growing on Ni-enriched ultramafic soils in South Africa, is perceived as a promising species for phytoremediation and phytomining due to its large biomass production and high Ni content. Total concentrations of a number of elements in mature leaves, soil and related bedrock were obtained. The average Ni concentration in leaves was 18,000 μg·g -1 dry mass, whereas in soil and bedrock the total amount of Ni was 1,300 μg·g-1 and 1,500 μg·g-1, respectively. Exceptionally high average Ni concentrations (55;000 ± 15;000 μg·g-1; n = 6) were found in B. coddii leaves from Songimvelo Game Reserve, including the highest-ever reported concentration of Ni in leaves (76;100 μg·g-1 - maximum value in a single sample). Young plants grown in pots with ultramafic soil accumulated small quantities of Cd, Pb or Zn, but the concentrations of these elements increased after the addition of metal solutions to the soil. Excised shoots immersed in concentrated solutions of Cd, Ni, Pb or Zn accumulated large amounts of these metals in the leaves. © Polish Academy of Sciences, Cracow 2004.},

note = {74},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0030369820,

title = {Cadmium-selenium antagonism and detoxifying enzymes in insects},

author = { A. Kędziorski and M. Nakonieczny and E. Świerczek and E. Szulińska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030369820&partnerID=40&md5=18eda2046c9653fdc406c19866c86013},

issn = {09370633},

year  = {1996},

date = {1996-01-01},

journal = {Fresenius' Journal of Analytical Chemistry},

volume = {354},

number = {5-6},

pages = {571-575},

abstract = {Activities of non-specific carboxylesterases (CarE) have been studied in adult males of hissing cockroach Gromphadorhina portentosa subjected to prolonged intoxication (7 weeks) with Cd and/or Se to investigate whether sublethal dose of cadmium (0.4 μmol, g-1 dry weight feed) would influence the detoxifying capabilities of the omnivorous insect, and whether selenium (0.01 μmol, g-1 dry weight feed) given concomitantly would act as Cd antagonist. Activities of CarE have been measured in crude homogenate, postmitochondrial supernatant and the pellet obtained from midgut, Malpighian tubules and fat body, using α-naphthyl acetate (αNA) and ρ-nitrophenyl acetate (ρNPA) as substrates. The highest rate of αNA hydrolysis occurred in midgut, whereas that of ρNPA in both the gut and Malpighian tubules. CarE activities in the Cd-group have been the highest in all tissues examined and for both substrates. A simultaneous administration of Cd and Se decreased the effects of Cd alone, probably as a result of partial elimination of the biologically active Cd pool. Selenium alone caused only minor effects when compared with cadmium. These results suggest, that disturbances of CarE activities depend on the Cd concentration in feed, and hence in tissues, as well as on possible interactions of the elements. © Springer-Verlag 1996.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0027724704,

title = {Functional aspects of cadmium and selenium interactions in insect digestive tract. Enzyme studies},

author = { M. Nakonieczny},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027724704&doi=10.1016%2fS0048-9697%2805%2980060-9&partnerID=40&md5=61fcdfcaf4f0c7c972c2b61940a18f0e},

doi = {10.1016/S0048-9697(05)80060-9},

issn = {00489697},

year  = {1993},

date = {1993-01-01},

journal = {Science of the Total Environment},

volume = {134},

number = {SUPPL. 1},

pages = {573-583},

abstract = {Effects of cadmium and selenium on some digestive and cytoplasmic enzymes activities in midgut of the cockroach (gromphadorhina portentosa) were evaluated after 7 weeks of intoxication. Activities of digestive enzymes were insignificantly lower in groups reared on food suplemented with cadmium (0.4 μmol Cd/g dry food) or selenium (0.01 μmol Se/g dry food). Significant differences relative to control groups were stated only in males, in the cases of α-amylase, cellobiase, sucrase and α-galactosidase. Activities of aminotransferases were significantly lower, when compared to control, only in females from Cd and Se groups. Alkaline phosphatase activity was very low or not detected in all examined groups, while that of acid phosphatase was lower in Cd and Cd + Se (0.4 μmol Cd + 0.01 μmol Se/g dry food) groups in comparison to control. Also, activity of carboxylesterase was significantly lower after Cd or Se intoxication. There were no differences, both in males and females, in this enzyme activity between control and Cd + Se groups. The results confirmed toxic effects of cadmium on enzymatic activity of the digestive tract in the cockroach, but they also indicated toxicity of selenium at the given dose. Simultaneous administration of cadmium and selenium reduced toxic effects exerted by these elements given separately. © 1993 Elsevier Science Publishers B.V.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}