@article{2-s2.0-85118248353,

title = {Evaluating the Accumulation of Antioxidant and Macro- and Trace Elements in Vaccinium myrtillus L.},

author = { M. Kandziora-Ciupa and M. Dabioch and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118248353&doi=10.1007%2fs12011-021-02989-4&partnerID=40&md5=de01039476ad444cbe53c36303b39f14},

doi = {10.1007/s12011-021-02989-4},

issn = {01634984},

year  = {2022},

date = {2022-01-01},

journal = {Biological Trace Element Research},

volume = {200},

number = {9},

pages = {4175-4185},

publisher = {Springer},

abstract = {This study was conducted in order to characterise the accumulation ability of Vaccinium myrtillus L for trace elements such as Al, Cd, Cu, Fe, Mn, Pb and Zn and selected macroelements Ca, K, Mg, Na and P. The accumulation of nutrient elements and trace elements (ANE and ATE) and changes in the ecophysiological responses in bilberry in differently polluted areas were compared. The accumulation of the elements in the roots, stems and leaves of bilberry from four sites (in the nearest vicinity of a zinc smelter; a Mining and Metallurgical Plant; a main road with a high traffic volume and an unprotected natural forest community) were measured using optical emission spectrometry with excitation using inductively coupled argon plasma after wet acid digestion. The highest Cd, Cu, Pb and Zn concentrations were found in the V. myrtillus samples that were growing under the influence of the emissions from the zinc smelter. Moreover, the level of the total accumulated trace metals (ATE—17.09 mmolc kg−1) was also highest for the bilberry at this site. However, in the same area, the sum of the accumulated macronutrients (ANE—296.92 mmolc kg−1) was lower than at the other sampling sites. An EF > 2 was found for Cd, Pb, Zn and Mn, which suggests that bilberries may be enriched in these metals. According to the translocation factor, V. myrtillus was an accumulator of Cd, Zn and Mn. An analysis of the ecophysiological responses showed that the greatest concentration of ascorbic acid was found in the leaves of V. myrtillus at the most contaminated site (3.32 mg g−1 fresh weight). There were no significant differences in the total phenols between the contaminated and non-contaminated sites. However, the lowest value of the total phenolic content (490.77 mg g−1 dry weight) was recorded at the site where the highest Fe concentration was detected in the leaves. A significantly positive correlation between the Cd, Pb and Zn concentrations and a strong negative correlation between the Mn concentration and ascorbic acid content in the leaves of bilberry was also observed. The results provide evidence that the ANE method, which is used to interpret the chemical composition of bilberry has made determining the impact of toxic trace metals on the mineral composition of V. myrtillus significantly easier and also that a non-enzymatic antioxidant such as ascorbic acid can be a good biomarker for determining the oxidative stress that is caused by toxic trace metals. © 2021, The Author(s).},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85127640859,

title = {Pollution and ecological risk assessment of heavy metals in forest soils with changes in the leaf traits and membrane integrity of Vaccinium myrtillus L.},

author = { M. Kandziora-Ciupa and J. Gospodarek and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85127640859&doi=10.1007%2fs10342-022-01446-8&partnerID=40&md5=848ced61e39a31080bda310ec9ae981a},

doi = {10.1007/s10342-022-01446-8},

issn = {16124669},

year  = {2022},

date = {2022-01-01},

journal = {European Journal of Forest Research},

volume = {141},

number = {3},

pages = {409-419},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {Vaccinium myrtillus L. is a plant that is tolerant to heavy metals that can grow in polluted and disturbed forest habitats and is a reliable pollution indicator. We aimed to assess the heavy metal bioaccumulation capacity of bilberry leaves, to assess the leaf traits and the ecophysiological responses to heavy metal stress in this species. We determined the accumulation of Cd, Cu, Fe, Mn, Ni, Pb and Zn in the leaves of Vaccinium myrtillus from four differently heavy metal-polluted forest sites. The highest Cd, Cu, Ni, Pb and Zn concentrations were found in bilberry leaves that had been exposed to emissions from a zinc smelter. Moreover, we found the highest levels of Cd, Pb and Zn in the soil at the same study site. The ecological risk was quantified using the potential ecological risk index (RI), taking into account the concentrations of heavy metals, the ecological risk factors and the toxic response factors. Generally, an extremely high ecological risk was estimated for two sampling sites (Miasteczko Śląskie and Bukowno) and a considerable ecological risk was estimated for one sampling site (Łosień). Additionally, we demonstrated that Cd is the metal that poses the highest ecological threat in the studied areas. The investigated heavy metals have significantly affected the area, width, perimeter, aspect ratio and roundness index of the studied bilberry leaves. Specifically, an increase of Mn in the blueberry leaves significantly reduced these parameters (apart from the aspect ratio). We observed that an increased bioaccumulation of the selected heavy metals in the leaves caused cell membrane damage and a higher malondialdehyde (MDA) content. The results and methods that were used in this study could be suitable for improving biomonitoring and can be widely applied in forest ecosystems, including heavy metal-polluted areas. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85120699286,

title = {Influence of lead on the activity of soil microorganisms in two Beskidy landscape parks},

author = { J. Borgulat and W. Łukasik and A. Borgulat and A. Nadgórska-Socha and M. Kandziora-Ciupa},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120699286&doi=10.1007%2fs10661-021-09503-2&partnerID=40&md5=e8cbaa7927751b5ae7fadf4e9eeca399},

doi = {10.1007/s10661-021-09503-2},

issn = {01676369},

year  = {2021},

date = {2021-01-01},

journal = {Environmental Monitoring and Assessment},

volume = {193},

number = {12},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {The aim of the study was to assess the potential impact of lead on soil metabolism in two landscape parks localized in the Beskid Śląski and Beskid Żywiecki mountains which were affected, among others, by air pollution from the Upper Silesian Industrial Region, the largest industrial zone in Poland. The study was carried out in six locations with different lead levels in the soil environment. Each plot was equipped with four pairs of vacuum ceramic lysimeters to assess the mobility of Pb in the soil. The metabolic activity was assessed by measuring: soil enzyme activity, soil respiration and by studying community-level physiological profiling (CLPP) using Biolog EcoPlates technique. The soil to the examination was collected near the stands with the lysimeters from two soil horizons (A and B layer). The analyses carried out showed that the factors that had the greatest influence on lead mobility were the organic carbon content and the soil pH. The elevated lead level in the topsoil (layer A) could affect the functional biodiversity of soil microorganisms, but low soil pH was a more likely limiting factor. In the subsoil (layer B), lower lead content was found and its probable effect on soil microbial activity was small. In summary, it can be concluded that the assessment of the influence of heavy metals on soil metabolism is not easy, and the Biolog system has proven to be a sensitive tool for assessing the potential impact of heavy metals on the soil environment. © 2021, The Author(s).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85119718841,

title = {The subsequent effects of soil pollution by petroleum products and its bioremediation on the antioxidant response and content of elements in vicia faba plants},

author = { J. Gospodarek and M. Rusin and M. Kandziora-Ciupa and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119718841&doi=10.3390%2fen14227748&partnerID=40&md5=f1c8bbed0e8da83ad023dfa520ed81b4},

doi = {10.3390/en14227748},

issn = {19961073},

year  = {2021},

date = {2021-01-01},

journal = {Energies},

volume = {14},

number = {22},

publisher = {MDPI},

abstract = {Petroleum-derived substances (PDSs), which are one of the most significant sources of energy, have become key factors that affect soils and, consequently, plants. The aim of this study was to determine the subsequent effects of soil contamination by PDSs such as petrol (P), diesel fuel (DF) and used engine oil (EO), in addition to its bioremediation using a ZB-01 biopreparation, on the activities of antioxidising enzymes as well as on the content of antioxidants in the leaves of Vicia faba L. The effects on the growth of the plants and their chemical composition were also determined. The results showed that as many as five years after contamination, engine oil and diesel fuel adversely affected the growth of plants. PDSs caused a significant increase in the activity of peroxidase and an increase in the content of proline. The contamination of the soil with oils (EO and DF) resulted in a decrease in the content of nutrients (Ca; Mg and P) in the plants. DF also decreased the content of K and N while EO decreased the content of Fe. PDSs also increased the content of lead and cadmium, and some resulted in a decrease in the content of zinc, manganese and copper. The ZB-01 biopreparation generally had a beneficial effect on the growth of plants, and contributed to a lowering of the activities of the analysed antioxidative enzymes as well as the content of antioxidants in plants in the soil that had been contaminated with diesel fuel. Furthermore, it most often caused an increase in the nutrient levels in the leaves of plants. The effect of the ZB-01 biopreparation on the content of heavy metals varied and was dependent on the specific contaminant and metal that were analysed. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85099910608,

title = {The influence of heavy metals on biological soil quality assessments in the Vaccinium myrtillus L. rhizosphere under different field conditions},

author = { M. Kandziora-Ciupa and A. Nadgórska-Socha and G. Barczyk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099910608&doi=10.1007%2fs10646-021-02345-1&partnerID=40&md5=0db83f629ed9108c477c38f680210eac},

doi = {10.1007/s10646-021-02345-1},

issn = {09639292},

year  = {2021},

date = {2021-01-01},

journal = {Ecotoxicology},

volume = {30},

number = {2},

pages = {292-310},

publisher = {Springer},

abstract = {The aim of this study was to determine the influence of heavy metals on biological soil quality assessments in Vaccinium myrtillus L. rhizosphere soil as well as in non-rhizosphere soil from different polluted sites. The presented study was also conducted in order to determine any differences in the soil physicochemical and biological properties between the Vaccinium rhizosphere soil and the non-rhizosphere soil. The content of heavy metals and their potential bioavailability, content of macronutrients, physicochemical soil properties, activity of six soil enzymes and microarthropod communities were determined. Soil organic matter, the levels of C, N and all the studied macronutrients and almost all enzyme activity were significantly higher in the rhizosphere soil than in the non-rhizosphere soil. At the most contaminated site, the content of heavy metals was also higher in the rhizosphere soil, but their bioavailability was lower than in the non-rhizosphere soil. The β-glucosidase and urease activity in the soil correlated most negatively with the examined metals. The levels of two enzymes were also strongly impacted by the organic matter—the C and N levels and pH. The number of microarthropods as well as the QBS (soil biological quality index) and FEMI (abundance-based fauna index) were higher in the rhizosphere soil. The bilberry rhizosphere soil had stronger correlation coefficient values between the measured parameters than the non-rhizosphere soil, which suggests that rhizosphere soil is more sensitive and could be used in the monitoring and assessment of forest ecosystems. β-glucosidase and urease were the most sensitive indicators of the adverse impact of Cd, Zn and Pb. The FEMI index seems to be a better indicator than the QBS for identifying differences in soil quality. © 2021, The Author(s).},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85124139328,

title = {Time-delayed effect of petroleum-derived products in soil and their bioremediation on plant - herbivore interaction},

author = { M. Rusin and J. Gospodarek and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85124139328&doi=10.24425%2faep.2021.138465&partnerID=40&md5=7676054f2feefc6b639a6829c49fa686},

doi = {10.24425/aep.2021.138465},

issn = {20834772},

year  = {2021},

date = {2021-01-01},

journal = {Archives of Environmental Protection},

volume = {47},

number = {3},

pages = {71-81},

publisher = {Polska Akademia Nauk},

abstract = {The aim of the study was to determine the time-delayed (after three years from the moment of soil pollution) effect of petroleum-derived products (PDPs) (petrol; diesel fuel and used engine oil) on the interaction between selected host plant (broad bean) and a herbivorous insect closely related to it (Sitona spp.). We assessed the condition of the plant exposed to pollutants (i.e. its growth and chemical composition), then we evaluated the attractiveness of the plant for both larvae and adults of the insect. The evaluation covered also the effect of bioremediation by using ZB-01 biopreparation. The results showed that after 3 years from soil contamination, engine oil and diesel fuel limited the feeding of adult sitona weevils while petrol caused increase in the attractiveness of plants for these insects. The PDPs negatively affected the growth of plants. The changes in element content depended on the type of pollutant. The biopreparation ZB-01 eliminated or reduced the differences caused by the presence of PDPs in the soil regarding the chemical composition of the host plant, and limited feeding by both the larvae and adult individuals of sitona weevils. The negative relationships between the contents of both some macroelements (Mg; S) and heavy metals (Zn; Ni), and feeding of imago of Sitona were observed. The obtained results indicate that PDPs remain for a long time in the environment and adversely affect not only the organisms directly exposed to the pollution - plants growing on polluted soil but also further links of the trophic chain, i.e. herbivores. © 2021. The Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY SA 4.0; https://creativecommons.org/licenses/by-sa/4.0/legalcode), which permits use, distribution, and reproduction in any medium, provided that the article is properly cited, the use is non-commercial, and no modifications or adaptations are made},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85106318242,

title = {The effect of petroleum-derived substances and their bioremediation on soil enzymatic activity and soil invertebrates},

author = { J. Gospodarek and M. Rusin and G. Barczyk and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106318242&doi=10.3390%2fagronomy11010080&partnerID=40&md5=4a112debfb8d95db90e2793871c01776},

doi = {10.3390/agronomy11010080},

issn = {20734395},

year  = {2021},

date = {2021-01-01},

journal = {Agronomy},

volume = {11},

number = {1},

publisher = {MDPI AG},

abstract = {Petroleum-derived substances (PDSs) as main pollutants of the natural environment can negatively affect the microbiological, biochemical, and biological properties of agricultural soils and, consequently, plant production. The present study aimed to determine the after-effect of PDSs such as petrol, used engine oil, and diesel fuel on the activity of selected soil enzymes (phosphatase; dehydrogenase; and urease) and on the occurrence of soil invertebrates. Moreover, changes in the analyzed parameters in response to bioremediation of the polluted soil by using ZB-01 preparation were investigated. The field experiments were performed four-and five-years post contamination. The results showed that even after five years, PDSs significantly modified the activity of soil enzymes; however, this effect was often varied, depending on the pollutant, enzyme, and time after soil contamination. Dehydrogenase seems to be a good indicator of soil contamination with PDSs, particularly diesel fuel. Engine oil and diesel fuel limited still the occurrence of soil invertebrates, particularly Collembola from the families Hypogastruidae, Isotomidae, and Entomobryidae, even after four and five years of contamination. This finding suggests the usefulness of these organisms in assessing soil pollution and in monitoring the progress of bioremediation. The effect of ZB-01 biopreparation on the activity of selected enzymes was varied. Its effect on the occurrence of soil invertebrates was usually beneficial, which was evident in diesel fuel-contaminated soil. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85093893479,

title = {The long-term effect of petroleum-derived substances and their bioremediation on the host plant (Vicia faba L.) and a herbivore (Sitona spp.)},

author = { J. Gospodarek and M. Rusin and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85093893479&doi=10.3390%2fagronomy10081066&partnerID=40&md5=8d3e393689f3efb44e24c7787d324946},

doi = {10.3390/agronomy10081066},

issn = {20734395},

year  = {2020},

date = {2020-01-01},

journal = {Agronomy},

volume = {10},

number = {8},

publisher = {MDPI AG},

abstract = {The present study assessed distant-in-time effects of soil contamination with petroleum-derived substances (PDSs) (petrol; diesel fuel and used engine oil) and their bioremediation using ZB-01 biopreparation on the growth of broad bean, content of nutrients and heavy metals in plants, and feeding by imagines and larvae of Sitona spp. The results showed that even after 5 years from the moment of soil contamination by engine oil and diesel fuel, they negatively impacted the growth of broad bean plants. PDSs significantly modified the content of nutrients and generally increased the content of heavy metals in plant organs. These substances also negatively affected the feeding of adult Sitona spp., causing a reduction in the percentage of plants damaged by beetles, and a decrease in the surface of consumed areas on leaves. The ZB-01 biopreparation had a generally positive effect on the morphological features of plants, and its effect on the content of nutrients and heavy metals was variable, depending on the type of contaminant, the analyzed metals or nutrients, and the involved part of the plant. The biopreparation also inhibited the adverse effect of PDSs on the feeding by imago of Sitona spp. © 2020 by the authors.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85079013982,

title = {Soil pollution by petroleum-derived substances and its bioremediation: The effect on aphis fabae scop. Infestation and antioxidant response in vicia faba l},

author = { M. Rusin and J. Gospodarek and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079013982&doi=10.3390%2fagronomy10010147&partnerID=40&md5=695324533fc78c62ecadc70153e52229},

doi = {10.3390/agronomy10010147},

issn = {20734395},

year  = {2020},

date = {2020-01-01},

journal = {Agronomy},

volume = {10},

number = {1},

publisher = {MDPI AG},

abstract = {In this study, the effects of soil contamination with petroleum-derived substances (PDSs) (petrol; diesel fuel and used engine oil) and its bioremediation using biopreparation ZB-01 on broad bean infestation by black bean aphid Aphis fabae Scop., as well as on the antioxidant enzymes activity (superoxide dismutase (SOD); catalase (CAT) and guaiacol peroxidase (POD)) and antioxidant (non-protein thiols and proline) content in plant leaves, were determined. Results showed that after three years from the moment of soil contamination PDSs limited infestation of broad bean by A. fabae. However, the adverse effects on aphids' life cycles were not proven. The lowered infestation may result from the lower attractiveness of contaminated plants to pests. PDSs significantly affected the activities of enzymes and the antioxidants content, with that effect being diversified. The increased activity of SOD was found in plants exposed to diesel fuel, together with the lowest numbers of aphids accompanying it, which can suggest a certain role of the enzyme in pest response to the stress caused by this PDS. The ZB-01 biopreparation limited the adverse effect of PDSs on the degree of broad bean plant infestation by A. fabae. Its influence on the antioxidant response was diversified. In the plants exposed to EO, changes in antioxidant response were reduced under the influence of ZB-01. © 2020 by the authors.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85086015206,

title = {Influence of soil contaminated with cadmium on cell death in the digestive epithelium of soil centipede Lithobius forficatus (Myriapoda, Chilopoda)},

author = { M.M. Rost-Roszkowska and I. Poprawa and Ł. Chajec and A. Chachulska-Żymełka and G. Wilczek and P. Wilczek and S. Student and M. Skowronek and A. Nadgórska-Socha and M. Leśniewska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086015206&doi=10.1080%2f24750263.2020.1757168&partnerID=40&md5=58f49682d112d8cec5ea24fe0e5c2b11},

doi = {10.1080/24750263.2020.1757168},

issn = {24750263},

year  = {2020},

date = {2020-01-01},

journal = {European Zoological Journal},

volume = {87},

number = {1},

pages = {242-262},

publisher = {Taylor and Francis Ltd.},

abstract = {Cadmium is a heavy metal that is treated as an environmental pollutant (air; water; soil). In order to understand the potential effects of cadmium in soil and soil invertebrates, it is important to describe all alterations which appear at different levels in organisms. The main aim of this study was to investigate, analyze and describe the alterations caused by cadmium short- and long-term intoxication at different levels in the organisms: from tissues to cells and organelles. In addition, the activation of cell death mechanisms that take part in homeostasis maintenance according to cadmium has been studied. Therefore, as the species for this project, a terrestrial and well-known widespread European species–the centipede Lithobius forficatus (Myriapoda; Chilopoda; Lithobiomorpha)–was chosen. This omnivorous species lives under upper layers of soil, under stones, litter, rocks, and leaves, and it is also commonly found in human habitats. The animals were divided into three groups: C–the control group, animals cultured in a horticultural soil; Cd1–animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl2, 12 days–short-term exposure; Cd2–animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl2, 45 days–long-term exposure. The midgut was isolated from each specimen and it was prepared for analysis using some histological, histochemical and immunohistochemical methods. Our studies showed that short-term intoxication causes intensification of autophagy and digestion of reserve material, while long-term exposure to this heavy metal causes activation of cell death processes together with inhibition of autophagy connected with the lack of reserve material. Additionally, we can infer that autophagy and cell death are nutrient deprivation-induced processes. Finally, we can conclude that short- and long-term exposure of soil centipede to cadmium affects different mechanisms and processes of cell death. © 2020, © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85078227387,

title = {Growth and chemical composition of Vicia faba L. Intercropped with insectary plants},

author = { J. Gospodarek and B. Biniaś and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078227387&doi=10.15244%2fpjoes%2f102375&partnerID=40&md5=3a6690f504b5db5ae56ed42b7a45d4a9},

doi = {10.15244/pjoes/102375},

issn = {12301485},

year  = {2020},

date = {2020-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {29},

number = {1},

pages = {601-608},

publisher = {HARD Publishing Company},

abstract = {The objective of the study was to find the impact of accompanying plants (sweet alyssum Lobularia maritima L. and white mustard Sinapis alba L.) upon the growth and chemical composition of broad bean (Vicia faba L.). The assessments were performed by applying the diversified row spacing of broad bean plants and with the use of thinning of the accompanying plant (white mustard) in order to avoid competition. Both accompanying plants beneficially affected broad bean growth. The nitrogen content in the leaves of broad beans accompanied by L. maritima with the retained standard row spacing of 50 cm and S. alba with a 65 cm row spacing significantly decreased (by approx. 6%), which can signify the competition for this element from both plants. The content of macroelements was diverse: in the case of Mg and P, the decreased content was noted, while the contents of Ca and K in some cases increased under the effect of the applied method of protection. The content of microelements and heavy metals usually either did not fluctuate widely, or increased (Fe; Ni; and Al). Only in the case of Zn was there evident decrease in its content in broad beans growing close to L. maritima plants. © 2020, HARD Publishing Company. All rights reserved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85068024771,

title = {Using Plantago major and Plantago lanceolata in environmental pollution research in an urban area of Southern Poland},

author = { I. Skrynetska and J. Karcz and G. Barczyk and M. Kandziora-Ciupa and R. Ciepał and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068024771&doi=10.1007%2fs11356-019-05535-x&partnerID=40&md5=9eb1239418337f92161e3c88b502ffbd},

doi = {10.1007/s11356-019-05535-x},

issn = {09441344},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {23},

pages = {23359-23371},

publisher = {Springer Verlag},

abstract = {The aim of this study was to perform a complex assessment of changes in the elements of an ecosystem that are caused by environmental pollution in industrial and urban biotopes. The study focused on three sites: a park, a road and the site of the metallurgical plant “Pokój” in the city of Ruda Śląska (Southern Poland), which are each under a different level of anthropogenic load. Soil and plant material samples (Plantago major and Plantago lanceolata leaves) were investigated by performing biochemical, ecophysiological and scanning electron microscopy (SEM) analyses. A significant difference was observed in all of the study samples. The content of Pb, Zn and Cd in the soil samples that had been collected at the site of the metallurgical plant exceeded the permitted limits (Cd > 4 mg kg−1; Pb > 100 mg kg−1; Zn > 300 mg kg−1). The content of Fe, Mn, Pb, Cd and Zn in the plant material was much higher in unwashed samples than in washed samples. The concentrations of potentially toxic elements (PTEs) were below the permitted level in the leaves of Plantago lanceolata for Cd (> 5 mg kg−1) and in the leaves of Plantago major for Zn (> 100 mg kg−1). The SEM observations revealed a significant decrease in the stomata pore length (SPL) in the Plantago lanceolata leaves that had been collected at the road site compared with the plants from the park site. The elemental content on the leaf surface was also determined using X-ray microanalysis. The total chlorophyll (Chl) content, ascorbic acid (AA), proline, guaiacol peroxidase (GPX) activity, pH, relative water content (RWC) and air pollution tolerance index (APTI) were evaluated. The APTI for the investigated species ranged from 5.6 to 7.4, which demonstrated that the studied plant species are sensitive to air pollutants. © 2019, The Author(s).},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85065462510,

title = {Effect of petroleum-derived substances and their bioremediation on triticum aestivum l. growth and chemical composition},

author = { J. Gospodarek and M. Rusin and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065462510&doi=10.15244%2fpjoes%2f91845&partnerID=40&md5=fe67dc21ddfe9ca7691fe650b26fbf11},

doi = {10.15244/pjoes/91845},

issn = {12301485},

year  = {2019},

date = {2019-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {28},

number = {4},

pages = {2131-2138},

publisher = {HARD Publishing Company},

abstract = {The aim of our studies was to determine the follow-up (four years after the soil contamination) effect of petroleum derived substances (PDSs), e.g., spent engine oil (EO), diesel fuel (DF), and petrol (P) on the growth of winter wheat (Triticum aestivum L.) and on the content of nutrients and heavy metals in plant leaves. We also evaluated the supportive effect of bioremediation by the use of ZB-01 biopreparation upon the parameters mentioned above. In general, the studied PDSs had not affected the growth of winter wheat, which is indicative of its relative resistance toward this kind of contaminant. PDSs significantly modified the contents of some nutrients in plant leaves: soil contamination with DF resulted in a decrease in sulphur content, but contamination with P led to the increase in carbon content with a simultaneous decrease in iron content in the analysed organs of plants. Furthermore, oils contributed to the increase in the manganese content in plants. The contaminants used in the experiments contributed to the increases in the contents of such heavy metals, i.e., zinc, lead, cadmium, and copper in the leaves of winter wheat; nevertheless, this phenomenon depended on the kind of PDSs, with stronger effects caused by oils. The application of ZB-01 biopreparation on soil contaminated with PDSs evoked changeable effects depending on the kind of contaminant and the analysed parameter. It generally resulted in levelling the negative effects of EO and DF. © 2019, HARD Publishing Company. All rights reserved.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85055325222,

title = {Antioxidant responses of Triticum aestivum plants to petroleum-derived substances},

author = { M. Rusin and J. Gospodarek and G. Barczyk and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85055325222&doi=10.1007%2fs10646-018-1988-3&partnerID=40&md5=18ec91e6beddc7a74493d79a44953c51},

doi = {10.1007/s10646-018-1988-3},

issn = {09639292},

year  = {2018},

date = {2018-01-01},

journal = {Ecotoxicology},

volume = {27},

number = {10},

pages = {1353-1367},

publisher = {Springer New York LLC},

abstract = {Winter common wheat (Triticum aestivum L.) plants were cultivated on petroleum products contaminated soils with and without using biopreparation ZB-01. We determined the impact of soil contamination with petrol, diesel fuel and engine oil on selected antioxidant enzymes and the levels of antioxidants in the leaves of winter wheat. The impact of petroleum products on selected morphological characteristics of the plants, levels of nutrients and heavy metals was also assessed. Winter wheat was relatively resistant to soil contamination with petroleum products, and did not show a significant impact on the morphological characteristics of the plants. The levels of nutrients and heavy metals in the plants depended on the type of pollutant and the analyzed component.‬ Biopreparation ZB-01 generally resulted in an increase in calcium levels in the plants.‬ The winter wheat plants growing in soil contaminated with engine oil were characterized by higher levels of zinc, lead, manganese and cadmium than the control plants.‬ Biopreparation applied to the soil contaminated with petrol resulted in a slight increase in the levels of lead and zinc in the plants.‬ The petroleum products affected the activity of antioxidant enzymes and the levels of antioxidants in the plants.‬ The general markers of soil contaminated with diesel fuel and petrol were POD activity and proline levels. Use of the ZB-01 biopreparation caused an increase in the levels of proline and -SH groups and an increase in the levels of carbon and calcium in the plants and had no effect on the morphological characteristics of plants.‬ © 2018, The Author(s).},

note = {22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85049975805,

title = {Effect of petroleum-derived substances on life history traits of bird cherry-oat aphid (Rhopalosiphum padi L.) and on the growth and chemical composition of winter wheat},

author = { M. Rusin and J. Gospodarek and A. Nadgórska-Socha and G. Barczyk and E. Boligłowa and M. Dabioch},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049975805&doi=10.1007%2fs11356-018-2723-6&partnerID=40&md5=99831e420938e636241ca70f5aab6206},

doi = {10.1007/s11356-018-2723-6},

issn = {09441344},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Science and Pollution Research},

volume = {25},

number = {27},

pages = {27000-27012},

publisher = {Springer Verlag},

abstract = {The aim of the study was to determine the effects of various petroleum-derived substances (PDSs), namely petrol, diesel fuel, and spent engine oil, on life history traits of the bird cherry-oat aphid Rhopalosiphum padi L., and on the growth and chemical composition of its host plant—winter wheat Triticum aestivum L. Each substance was tested separately, using two concentrations (9 and 18 g kg−1). Plants were cultivated in both control and contaminated soils. In early October 2013, soil was contaminated and after 1 week, winter wheat seeds, ‘Batuta’ cultivar, were sown. In early June 2014, observations of the effect of petroleum-derived substances on traits of three successive generations of aphids were conducted. Aphids were inoculated separately on leaves using cylindrical cages hermetically closed on both sides. Contamination of aphid occurred through its host plant. Results showed that all of the applied petroleum-derived substances have a generally adverse effect on the developmental parameters in aphids, resulting in the decrease of its fecundity, shortening its average life span, and most often lowering of the population intrinsic growth rate. PDSs caused the limitation of growth in wheat plants; whereas, changes in nutrient contents and heavy metals depended on the part of the plant analysed, the substance applied, and on its dose. The negative relationships between the contents of both some macro-elements (Ca; K; P) and heavy metals (Mn; Cd; Cu; and Zn) and the developmental parameters of particular generations of R. padi were observed. The high susceptibility of R. padi to the presence of PDSs in the substrate for the host plant should be emphasised—the clear-cut changes in the life span and fecundity, with relatively small changes in the chemical composition of the plant, constitute an evident indication that the developmental parameters of aphids have the potential for the use as bio-indicator to evaluate the state of the environment contaminated by PDSs. © 2018, The Author(s).},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85045523674,

title = {Ecophysiological Responses to Environmental Pollution of Selected Plant Species in an Industrial Urban Area},

author = { I. Skrynetska and R. Ciepał and M. Kandziora-Ciupa and G. Barczyk and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045523674&doi=10.1007%2fs41742-018-0088-9&partnerID=40&md5=4545d7596111f07f0147dc0c345b032e},

doi = {10.1007/s41742-018-0088-9},

issn = {17356865},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Environmental Research},

volume = {12},

number = {2},

pages = {255-267},

publisher = {Springer International Publishing},

abstract = {The aim of the study was to determine the state of the environment in Sosnowiec (southern Poland) based on the ratio of air pollution tolerance index (APTI) and the determination of concentrations of selected metals in soil and plant material. The study was conducted in ten stands: five of them were identified as ‘polluted’ and five as ‘clean’ stands. The metal content was evaluated by the atomic absorption method. For APTI calculation, analysis of total chlorophyll concentration and relative water content, the pH of the extract from the leaves was used. Analysis of the bioaccumulation of metals in the leaves revealed excessive concentrations of Pb (32.4 mg kg−1) and Zn (129.78 mg kg−1) at the KWK (coal mine) and Las (forest) stands. Higher capacity accumulation of Pb and Fe was characteristic for Plantago lanceolata. The permitted content of Pb (762.6 mg kg−1) in the soil samples collected at the Cedler stand was exceeded. Elevated levels, higher than the permissible concentration of Cd, were documented in soil samples from most stands within the city. In soil samples collected at the Cedler stand, Cd concentration was almost 15 times higher than acceptable for soil (4 mg kg−1). Based on the scope of the APTI 4.4–9.42 index obtained for the studied species, they should be considered sensitive plants. All selected species can be used as bioindicators for environmental pollution. © 2018, The Author(s).},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85020551146,

title = {Bioaccumulation of heavy metals and ecophysiological responses to heavy metal stress in selected populations of Vaccinium myrtillus L. and Vaccinium vitis-idaea L},

author = { M. Kandziora-Ciupa and A. Nadgórska-Socha and G. Barczyk and R. Ciepał},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020551146&doi=10.1007%2fs10646-017-1825-0&partnerID=40&md5=10231af4d736209fc6b9aeee77c9e2c7},

doi = {10.1007/s10646-017-1825-0},

issn = {09639292},

year  = {2017},

date = {2017-01-01},

journal = {Ecotoxicology},

volume = {26},

number = {7},

pages = {966-980},

publisher = {Springer New York LLC},

abstract = {The aim of this study was to determine the concentrations of heavy metals (Cd; Pb; Zn; Fe; and Mn) in soil, and their bioavailability and bioaccumulation in Vaccinium myrtillus L. and Vaccinium vitis-idaea L. organs. Analysis also concerned the physiological responses of these plants from three polluted sites (immediate vicinity of a zinc smelter in Miasteczko Śląskie; ArcelorMittal Poland S.A. iron smelter in Dąbrowa Górnicza-Łosień; and Jaworzno III power plant in Jaworzno) and one pseudo-control site (Pazurek nature reserve in Jaroszowiec Olkuski). All of the sites are situated in the southern parts of Poland in the Śląskie or Małopolskie provinces. The contents of proline, non-protein thiols, glutathione, ascorbic acid, and the activity of superoxide dismutase and guaiacol peroxidase in the leaves of Vaccinium myrtillus L. and Vaccinium vitis-idaea L. were measured. In soil, the highest levels of Cd, Pb, and Zn (HNO3 extracted and CaCl2 extracted) were detected at the Miasteczko Śląskie site. At all sites a several times lower concentration of the examined metals was determined in the fraction of soil extracted with CaCl2. Much higher Cd, Pb, Zn and Fe concentrations were found in V. myrtillus and V. vitis-idaea grown at the most polluted site (located near the zinc smelter) in comparison with cleaner areas; definitely higher bioaccumulation of these metals was found in lingonberry organs. Additionally, we observed a large capability of bilberry to accumulate Mn. Antioxidant response to heavy metal stress also differed between V. myrtillus and V. vitis-idaea. In V. myrtillus we found a positive correlation between the level of non-protein thiols and Cd and Zn concentrations, and also between proline and these metals. In V. vitis-idaea leaves an upward trend in ascorbic acid content and superoxide dismutase activity accompanied an increase in Cd, Pb, and Zn concentrations. At the same time, the increased levels of all tested metals in the leaves of V. vitis-idaea were accompanied by a decreased activity of guaiacol peroxidase. In both species increased Mn accumulation caused a decrease in antioxidant response. © 2017, The Author(s).},

note = {31},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85011271393,

title = {Effect of petroleum-derived substances on life history traits of black bean aphid (Aphis fabae Scop.) and on the growth and chemical composition of broad bean},

author = { M. Rusin and J. Gospodarek and A. Nadgórska-Socha and G. Barczyk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85011271393&doi=10.1007%2fs10646-017-1764-9&partnerID=40&md5=50a568119a5ea34cd551b16c6e80e52c},

doi = {10.1007/s10646-017-1764-9},

issn = {09639292},

year  = {2017},

date = {2017-01-01},

journal = {Ecotoxicology},

volume = {26},

number = {3},

pages = {308-319},

publisher = {Springer New York LLC},

abstract = {The aim of the study was to determine the effects of various petroleum-derived substances, namely petrol, diesel fuel and spent engine oil, on life history traits and population dynamics of the black bean aphid Aphis fabae Scop. and on growth and chemical composition of its host plant Vicia faba L. Each substance was tested separately, using two concentrations (9 g kg−1 and 18 g kg−1). The experiment was conducted in four replications (four pots with five plants in each pot per treatment). Plants were cultivated in both control and contaminated soils. After six weeks from soil contamination and five weeks from sowing the seeds, observations of the effect of petroleum-derived substances on traits of three successive generations of aphids were conducted. Aphids were inoculated separately on leaves using cylindrical cages hermetically closed on both sides. Contamination of aphid occurred through its host plant. Results showed that all tested substances adversely affected A. fabae life history traits and population dynamics: extension of the prereproductive period, reduction of fecundity and life span, reduction of the population intrinsic growth rate. In broad bean, leaf, roots, and shoot growth was also impaired in most conditions, whereas nutrient and heavy metal content varied according to substances, their concentration, as well as plant part analysed. Results indicate that soil contamination with petroleum-derived substances entails far-reaching changes not only in organisms directly exposed to these pollutants (plants), but also indirectly in herbivores (aphids) and consequently provides information about potential negative effects on further links of the food chain, i.e., for predators and parasitoids. © 2016, The Author(s).},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85031746278,

title = {Epiphytic habitats in an urban environment; Contamination by heavy metals and sulphur in the barks of different tree species},

author = { B. Fojcik and M. Chruścińska and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031746278&partnerID=40&md5=eee7441a6e4a9e634809bf0687f8d345},

issn = {16439953},

year  = {2017},

date = {2017-01-01},

journal = {Polish Journal of Natural Sciences},

volume = {32},

number = {2},

pages = {283-295},

publisher = {University of Warmia and Mazury in Olsztyn},

abstract = {Air pollution is mentioned as one of the major factors that limit the occurrence of epiphytes in urban areas. The purpose of the investigation was to analyze any dissimilarity in the amount of heavy metals and sulphur to the bark of different tree species that had been subjected to bark pollution (pH values were also analyzed). In order to estimate the differences in the amount of pollutants that had reached the bark, the "moss-bag" method was also used. The present study confirms that the barks of tree species differ in the level of pollutants (such as heavy metals and sulphur). The higher pH values of the bark appears to be an important buffer for the adverse effects of air pollution and determines the recolonization processes of epiphytic species in urban areas.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85019740170,

title = {Air pollution tolerance index and heavy metal bioaccumulation in selected plant species from urban biotopes},

author = { A. Nadgórska-Socha and M. Kandziora-Ciupa and M. Trzęsicki and G. Barczyk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019740170&doi=10.1016%2fj.chemosphere.2017.05.128&partnerID=40&md5=abd25229b41acf4d1b9c41a5e8cb42b1},

doi = {10.1016/j.chemosphere.2017.05.128},

issn = {00456535},

year  = {2017},

date = {2017-01-01},

journal = {Chemosphere},

volume = {183},

pages = {471-482},

publisher = {Elsevier Ltd},

abstract = {This research was carried out on plants Taraxacum officinale, Plantago lanceolata, Betula pendula and Robinia pseudoacacia growing in urban biotopes with different levels of heavy metal contamination in the city of Dąbrowa Górnicza (southern Poland). Based on the pollution index, the highest heavy metal contamination was determined in the site 4 (connected with industry emitters) and 6 (high traffic). The metal accumulation index (MAI) values ranged within the biotopes in Dąbrowa Górnicza between 7.3 and 20.6 for R. pseudoacacia, 4.71–23.1 for P. lanceolata, 4.68–28.1 for T. officinale and 10.5–27.2 for B. pendula. Increasing tendency in proline content in biotopes connected with high traffic was found in the leaves of investigated plants (except R. pseudoacacia). Similar tendency was observed for ascorbic acid content in the foliage of the plants as well as in T. officinalle in stands connected industrial emission. Non-protein thiols content increased especially in the leaves of R. pseudoacacia in biotopes with high traffic emissions as well as in T. officinale in stands connected with industry. The mean values of APTI (Air Pollution Tolerance Index) within the city of Dąbrowa Górnicza for investigated plants were found in the following ascending order P. lanceolata < R. pseudoacacia < B. pendula < T. officinale. Among the investigated plants B. pendula and T. officinale may be postulated as appropriate plants in urban areas with considerable soil and air contamination, especially with heavy metals. The results indicate that species deemed tolerant according to APTI are suitable plants in barriers areas to combat atmospheric pollution. © 2017 Elsevier Ltd},

note = {70},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84975789752,

title = {Robinia pseudoacacia and Melandrium album in trace elements biomonitoring and air pollution tolerance index study},

author = { A. Nadgórska-Socha and M. Kandziora-Ciupa and R. Ciepał and G. Barczyk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975789752&doi=10.1007%2fs13762-016-1010-7&partnerID=40&md5=b584f6f400f48ca26657f0005f724b82},

doi = {10.1007/s13762-016-1010-7},

issn = {17351472},

year  = {2016},

date = {2016-01-01},

journal = {International Journal of Environmental Science and Technology},

volume = {13},

number = {7},

pages = {1741-1752},

publisher = {Center for Environmental and Energy Research and Studies},

abstract = {The accumulation efficiency of selected trace elements in the leaves of Melandrium album and Robinia pseudoacacia grown on heavy metal contaminated sites in comparison with a non-contaminated one was evaluated. The study was undertaken to calculate air pollution tolerance index and to determine the contents of selected metabolites: glutathione, non-protein thiols, ascorbic acid, chlorophyll and the activity of antioxidant enzymes: guaiacol peroxidase and superoxide dismutase. Such estimations can be useful in better understanding of plants defense strategies and potential to grow in contaminated environments. The results in the most contaminated site revealed higher contents of metals in M. album leaves, especially Zn, Cd and Pb (3.4; 6 and 2.3 times higher; respectively) in comparison with the R. pseudoacacia. Better accumulation capacity found in M. album was shown by metal accumulation index values. The plants could be used as indicators of Zn, Cd (both species) and Pb (M. album) in the soil. Glutathione content (in both species) and peroxidase activity (in M. album), general markers of heavy metals contamination, were increased in contaminated sites. In most cases in contaminated areas R. pseudoacacia had decreased ascorbic acid and chlorophyll levels. Opposite tendency was recorded in M. album leaves, where similar or higher contents of the above-mentioned metabolites were found. In our study, M. album and R. pseudoacacia proved to be sensitive species with the air pollution tolerance index lower than 11 and can be recommended as bioindicators. © 2016, The Author(s).},

note = {21},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84961992262,

title = {Accumulation of heavy metals and antioxidant responses in Pinus sylvestris L. needles in polluted and non-polluted sites},

author = { M. Kandziora-Ciupa and R. Ciepał and A. Nadgórska-Socha and G. Barczyk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961992262&doi=10.1007%2fs10646-016-1654-6&partnerID=40&md5=f963e026c4b1aac9378b084156486c54},

doi = {10.1007/s10646-016-1654-6},

issn = {09639292},

year  = {2016},

date = {2016-01-01},

journal = {Ecotoxicology},

volume = {25},

number = {5},

pages = {970-981},

publisher = {Springer New York LLC},

abstract = {The purpose of this study was to determine the concentrations of heavy metals (cadmium; iron; manganese; lead and zinc) in current-year, 1-year old and 2-year old needles of Pinus sylvestris L. Trees were from three heavily polluted (immediate vicinity of zinc smelter; iron smelter and power plant) and three relatively clean sites (nature reserve; ecologically clean site and unprotected natural forest community) in southern Poland. Analysis also concerned the antioxidant response and contents of protein, proline, total glutathione, non-protein thiols and activity of guaiacol peroxidase (GPX) in the needles. Generally, in pine needles from the polluted sites, the concentrations of the metals were higher and increased with the age of needles, and in most cases, antioxidant responses also were elevated. The highest levels of Cd, Pb and Zn were found in 2-year old pine needles collected near the polluted zinc smelter (respectively: 6.15; 256.49; 393.5 mg kg−1), Fe in 2-year old pine needles in the vicinity of the iron smelter (206.82 mg kg−1) and Mn in 2-year old needles at the ecologically clean site (180.32 mg kg−1). Positive correlations were found between Fe, Mn and Pb and the content of proteins and NPTs, between Cd and non-protein –SH groups, and between Zn and proline levels. The activity of GPX increased under the influence of Mn, while glutathione levels tended to decrease as Mn levels rose. The data obtained show that the levels of protein and non-protein –SH groups may be useful in biological monitoring, and that these ecophysiological parameters seem to be good evidence of elevated oxidative stress caused by heavy metals. © 2016, The Author(s).},

note = {33},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85008881292,

title = {Chemical composition of broad beans (Vicia faba L.) and development parameters of black bean aphid (Aphis fabae Scop.) under conditions of soil contamination with oil derivatives},

author = { J. Gospodarek and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85008881292&doi=10.5601%2fjelem.2015.20.1.770&partnerID=40&md5=e57d0ffed153f021a72d107147ac7891},

doi = {10.5601/jelem.2015.20.1.770},

issn = {16442296},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Elementology},

volume = {21},

number = {4},

pages = {1359-1376},

publisher = {Polish Society Magnesium Research},

abstract = {The indirect effect of oil derivatives, that is arising from the polluted soil through a host plant to the phytophagous fauna feeding on the plant, has not been investigated before. Hence, our goal has been to assess the effect of used engine oil, diesel fuel and petrol on the chemical composition of broad beans cultivated in soil polluted with these substances and on development parameters, such as fecundity, life span and intrinsic rate of natural increase, in a population of black bean aphid (Aphis fabae Scop.). Doses: 3 g kg-1 (dose I) and 6 g kg-1 (dose II) of an oil derivative per 1 kg of soil d.m. were used. Broad bean (Vicia faba L.) cv. White Windsor was chosen as the test plant used for studying the biology of aphids. When the experiment was completed, an analysis of selected elements (Zn; Fe; Mn; Ni; Cu; Pb; Cd; N; Ca and Mg) was conducted in leaves of broad bean. Soil pollution with petrol in dose I led to a significant decrease in the content of Ca, Mg, Fe, Mn, Zn, Ni and Cu, while causing a marked increase in the Pb content. The double dose of petrol (6 g kg-1) additionally contributed to diminishing the N content, whereas the concentrations of Ni and Cd increased significantly after its application. Soil pollution with diesel fuel, irrespective of a dose, caused a significant decline in the Ca, N, Zn, Ni and Cu concentrations and an increase in the Pb content. Additionally, the dose of 6 g kg-1 led to an increase in the Mg, Fe and Cd content in broad bean plants. Soil pollution with used engine oil dosed at 3 g kg-1 led to a reduction of the Ca, Mg, Fe, Mn, Zn and Cu content, while causing an increase in Pb and a slight increase in N concentrations. The double dose of engine oil caused a decline only in the Ca, Mn, Ni and Cu content. The effect of oil derivatives on the biology of black bean aphid was more pronounced in the second generation. All the analysed substances, except for engine oil in the lower dose, significantly decreased the intrinsic rate of natural increase in the population. © 2016, Polish Society Magnesium Research. All rights reserved.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84959147239,

title = {Enzymatic activities and arbuscular mycorrhizal colonization of Plantago lanceolata and Plantago major in a soil root zone under heavy metal stress},

author = { E. Gucwa-Przepióra and A. Nadgórska-Socha and B. Fojcik and D. Chmura},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84959147239&doi=10.1007%2fs11356-015-5695-9&partnerID=40&md5=57c4de2af919d51e1a78023956f99a44},

doi = {10.1007/s11356-015-5695-9},

issn = {09441344},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Science and Pollution Research},

volume = {23},

number = {5},

pages = {4742-4755},

publisher = {Springer Verlag},

abstract = {The objectives of the present field study were to examine the soil enzyme activities in the soil root zones of Plantago lanceolata and Plantago major in different heavy metal contaminated stands. Moreover, the investigations concerned the intensity of root endophytic colonization and metal bioaccumulation in roots and shoots. The investigated Plantago species exhibited an excluder strategy, accumulating higher metal content in the roots than in the shoots. The heavy metal accumulation levels found in the two plantain species in this study were comparable to other plants suggested as phytostabilizers; therefore, the selected Plantago species may be applied in the phytostabilization of heavy metal contaminated areas. The lower level of soil enzymes (dehydrogenase; urease; acid; and alkaline phosphatase) as well as the higher bioavailability of metals in the root zone soil of the two plantain species were found in an area affected by smelting activity, where organic matter content in the soil was also the smallest. Mycorrhizal colonization on both species in the contaminated area was similar to colonization in non-contaminated stands. However, the lowest arbuscule occurrence and an absence of dark septate endophytes were found in the area affected by the smelting activity. It corresponded with the lowest plant cover observed in this stand. The assessment of enzyme activity, mycorrhizal colonization, and the chemical and physical properties of soils proved to be sensitive to differences between sites and between Plantago species. © 2015, The Author(s).},

note = {26},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85037860665,

title = {Assessment of heavy metals contamination and enzymatic activity in pine forest soils under different levels of anthropogenic stress},

author = { M. Kandziora-Ciupa and R. Ciepał and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85037860665&doi=10.15244%2fpjoes%2f61813&partnerID=40&md5=0592f8773359181ee95dcfee5d41df99},

doi = {10.15244/pjoes/61813},

issn = {12301485},

year  = {2016},

date = {2016-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {25},

number = {3},

pages = {1045-1051},

publisher = {HARD Publishing Company},

abstract = {The aim of this work was to assess heavy metal concentrations and the effects of these metals on soil enzymatic activity in polluted and potentially unpolluted forest areas. The study was performed in typical pine forests located in three heavily polluted (in the immediate vicinity of a zinc smelter; an iron smelter; and a power plant) and three relatively clean sites (a nature reserve; an ecological site; and an unprotected natural forest community) in southern Poland. The research concerned the activity of acid phosphatase, dehydrogenase, β-glucosidase, and urease. In the soils, taken from the top 0-10 cm layer, we also tested the concentrations of heavy metals (Cd; Fe; Pb; and Zn) extracted with 10% HNO3 and 0.01 M CaCl2, and their pH and organic matter contents. Single pollution index and Nemerow pollution index were also calculated. Nemerow pollution index indicated serious pollution with heavy metals at two sites. The lowest activity of soil enzymes (acid phosphatase; and in particular β-glucosidase) was found in the site with the highest levels of heavy metals. In this study we found no effect of organic matter on the activity of the selected enzymes. There was a significant effect of pH on the activity of acid phosphatase and β-glucosidase. © 2016, HARD Publishing Company. All rights reserved.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84934949840,

title = {Element accumulation, distribution, and phytoremediation potential in selected metallophytes growing in a contaminated area},

author = { A. Nadgórska-Socha and M. Kandziora-Ciupa and R. Ciepał},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934949840&doi=10.1007%2fs10661-015-4680-6&partnerID=40&md5=8b31987e1a255c5a754ce8b05500d55f},

doi = {10.1007/s10661-015-4680-6},

issn = {01676369},

year  = {2015},

date = {2015-01-01},

journal = {Environmental Monitoring and Assessment},

volume = {187},

number = {7},

publisher = {Kluwer Academic Publishers},

abstract = {The distribution of elements in three pseudometallophytes species Cardaminopsis arenosa, Plantago lanceolata, and Plantago major, naturally occurring at metalliferous and non-metalliferous sites in southern Poland, was investigated. The accumulation of Al, Cd, Cu, Fe, Mn, Pb, Zn, as well as Ca, P, Na, and K in shoots and roots was measured. The level of the accumulated trace elements (ATE) was visibly higher in C. arenosa and P. lanceolata from metalliferous sites than non-contaminated ones. However, the level of the accumulated nutrient elements (ANE) was visibly higher only in C. arenosa plants. Also, higher potassium share in ANE was found in the shoots of C. arenosa and Plantago species from metalliferous sites than non-contaminated ones. The highest content of Cd, Zn, Pb, Al, Fe, and Mn was found in C. arenosa, which better reflected metal concentrations in the metalliferous and non-metalliferous soil than other plants. In the studied Plantago species, in almost all cases in all sites TF (translocation coefficient) and MR (mobility ratio) were below 1, which indicates they use the excluder strategy. The best accumulation ability was found for C. arenosa. The higher translocation coefficients (TF > 1) for Zn and Cd in C. arenosa shoots make it suitable for phytoextraction from soil, while the lower translocation ratios (TF < 1) for Zn and Cd in Plantago species and also for Pb in C. arenosa make them suitable for phytostabilization. Almost in all cases the plants had enrichment coefficient >2, which suggested that they may act as indicators of the soil metal contamination. © 2015, Springer International Publishing Switzerland.},

note = {22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84942057301,

title = {The effect of petroleum-derived substances on the growth and chemical composition of Vicia faba L},

author = { M. Rusin and J. Gospodarek and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942057301&doi=10.15244%2fpjoes%2f41378&partnerID=40&md5=c84d996d8bb294ffbe0c978d38cc80d8},

doi = {10.15244/pjoes/41378},

issn = {12301485},

year  = {2015},

date = {2015-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {24},

number = {5},

pages = {2157-2166},

publisher = {HARD Publishing Company},

abstract = {Petroleum and petroleum-derived products can contribute to the inhibition of growth and development of plants. Soil contamination by these compounds either hampers or makes difficult the intake of water and mineral salts from the substrate, and also breaks a number of metabolic processes. As a result of the deficiency of chlorophyll and nutrients, the affected plants grow deformed roots, shoots, leaves, and flowers, and show numerous chloroses and necroses. The objective of our research was to determine the effects of petrol, used engine oil, and diesel oil on select morphological parameters of Vicia faba L., and on the content of select nutrients and heavy metals in the leaves of plants. The effect of the bioremediation process initiated by biopreparation ZB-1 on the above-mentioned parameters was also determined. The analyzed plants were obtained from the Experimental Station of the University of Agriculture in Cracow, located in Mydlniki near Cracow. In June 2010, the soil placed in special containers was contaminated with petroleum-derived substances at a quantity of 6,000 mg per 1 kg of dry mass, by pouring it on the soil. Half of the objects were subjected to bioremediation. The evaluation of the morphology of plants and the structural elements of yield was made at the stage of technological maturity of broad bean seeds. The evaluation of heavy metals content was conducted using flame absorption spectrometry. The carbon, nitrogen, and sulphur content was determined in a Variomax CNS analyzer. The results of the experiment showed that diesel oil had the most adverse effects on the morphological parameters of plants. Engine oil caused a decrease in the nitrogen, carbon, and sulfur contents in the leaves of plants, but on the other hand this substance, like other petroleum-derived compounds, increased the content of most heavy metals. The effect of biopreparation was diverse and depended on the type of substance and on the analyzed parameter. The conducted experiment showed that petroleum-derived substances continued to adversely affect the growth of plants even three years after soil contamination. Additionally, it increased the accumulation of harmful substances in the organs of plants which could, in turn, seriously impact the economy and human health. However, the intensity of the impact depends on the type of derivative. Bioremediation supported by the use of microorganisms is an advantageous solution that permits the improving of the growth parameters of plants, as well as offsetting the harmful effects of petroleum-derived products upon the majority of the analyzed elements. © 2015, Polish Journal of Environmental Studies. All rights reserved.},

note = {34},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84886013557,

title = {Heavy metal bioaccumulation and antioxidative responses in Cardaminopsis arenosa and Plantago lanceolata leaves from metalliferous and non-metalliferous sites: A field study},

author = { A. Nadgórska-Socha and B. Ptasiński and A. Kita},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886013557&doi=10.1007%2fs10646-013-1129-y&partnerID=40&md5=84289552fdf4a06b7c07bb57e93702fb},

doi = {10.1007/s10646-013-1129-y},

issn = {09639292},

year  = {2013},

date = {2013-01-01},

journal = {Ecotoxicology},

volume = {22},

number = {9},

pages = {1422-1434},

abstract = {The purpose of this study was to determine the concentrations of heavy metals (cadmium; lead; zinc; copper; iron and manganese) in soil, their bioavailability and bioaccumulation in plants leaves. This study also examined their influences on the antioxidant response of the plants Cardaminopsis arenosa and Plantago lanceolata grown in metal-contaminated and non-contaminated soils. The activities of guaiacol peroxidase and superoxide dismutase and the levels of antioxidants such as glutathione, proline and non-protein thiols were measured. Concentrations of the examined metals were several to thousands of times lower in the potentially bioavailable fraction than in the acid-extracted fraction of the soil. Similar mode of antioxidant responses in plant leaves of metalliferous populations indicates the tolerance of plants towards heavy metals. However POD and GSHt had a particularly strong role in defense reactions, as their increase was the most common reaction to heavy metal contamination.The levels of Zn, Cd and Pb in the leaves of C. arenosa better reflected metal concentrations in the metalliferous and non-metalliferous soil than the determined metal concentrations in P. lanceolata. Bioaccumulated Zn, Cd and Pb concentrations were above or in the ranges mentioned as toxic for plant tissues and therefore the studied plants have potential for use in phytostabilization. © 2013 The Author(s).},

note = {87},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84873081848,

title = {Accumulation of heavy metals and antioxidant responses in Vicia faba plants grown on monometallic contaminated soil},

author = { A. Nadgórska-Socha and A. Kafel and M. Kandziora-Ciupa and J. Gospodarek and A. Zawisza-Raszka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84873081848&doi=10.1007%2fs11356-012-1191-7&partnerID=40&md5=cefa722cf675bb1323263001c0003b2e},

doi = {10.1007/s11356-012-1191-7},

issn = {09441344},

year  = {2013},

date = {2013-01-01},

journal = {Environmental Science and Pollution Research},

volume = {20},

number = {2},

pages = {1124-1134},

publisher = {Springer Verlag},

abstract = {The purpose of this study was to explore the effects of soil contamination by selected metals (cadmium; copper; nickel; lead or zinc) on the antioxidant response of Vicia faba plants. The levels of the antioxidants: glutathione, proline, non-protein thiols, as well as guaiacol peroxidase and catalase activities were measured in the upperparts of plants. Additionally, the potential bioavailability of metals in the soil and their concentrations in V. faba plants were compared. Treatment with metal caused the problem of an elevation in its bioavailability in soil and its concentration in leaves and stems. The most serious problems seemed to be metal elevations in soil, especially Zn and Ni as well as in the aerial parts of V. faba plants. The antioxidant responses appeared to be metal specific. The elevation of guaiacol peroxidase activity in leaves and stems as well as the proline in leaves was the only more general reaction to metal exposure. Upon analysis of the effects of soil metal contamination on V. faba plants, we recommend the use of some measurements such as guaiacol peroxidase activity and proline level as useful tools in biological monitoring. © 2012 The Author(s).},

note = {84},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84879685581,

title = {A comparative study of heavy metal accumulation and antioxidant responses in Vaccinium myrtillus L. leaves in polluted and non-polluted areas},

author = { M. Kandziora-Ciupa and R. Ciepał and A. Nadgórska-Socha and G. Barczyk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84879685581&doi=10.1007%2fs11356-012-1461-4&partnerID=40&md5=dba706313183a23c0d907c161c624133},

doi = {10.1007/s11356-012-1461-4},

issn = {09441344},

year  = {2013},

date = {2013-01-01},

journal = {Environmental Science and Pollution Research},

volume = {20},

number = {7},

pages = {4920-4932},

publisher = {Springer Verlag},

abstract = {The purpose of this study was to explore a possible relationship between the availability of metals in soil (Cd; Fe; Mn; Pb and Zn) and their concentrations in leaves of Vaccinium myrtillus L. as a species which has been reported to be a successful colonist of acid-and-heavy metal-contaminated soil. Analysis also concerned the antioxidant response of plants from three heavily polluted (immediate vicinity of: zinc smelter; iron smelter and power plant) and three relatively clean sites (nature reserve; ecological site and unprotected natural forest community) in southern Poland. The contents of glutathione, non-protein thiols, protein, proline and activity of guaiacol peroxidase in leaves of bilberry were measured. Generally, the concentrations of metals in the HNO 3 and CaCl 2 extracants of the soil from the polluted sites were higher. Moreover, the antioxidant responses were also elevated in bilberries in the polluted sites. Significant positive relationships between Cd, Pb and Zn concentrations in soil and in the plants were found. In the leaves of V. myrtillus from the polluted sites, higher concentrations of Cd, Pb and Zn were noted (In Miasteczko Śląskie respectively 6.26; 157.09 and 207.17 mg kg -1 d.w.). We found a positive correlation between the increase in the NPTs and protein contents as well as the Cd, Pb and Zn concentrations in V. myrtillus. Cd, Pb and Zn also decreased guaiacol peroxidase activity. However, the activity of this enzyme increased under Fe. A decreasing trend in glutathione contents was observed with increasing iron and manganese concentrations in bilberry leaves. Parameters such as protein, non-protein -SH groups and changes in GPX activity seem to be universal, sensitive and correlated well with heavy metal stress. © 2013 The Author(s).},

note = {53},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-80054000861,

title = {Assessment of bark reaction of select tree species as an indicator of acid gaseous pollution},

author = { K.A. Bierza and B.F. Palowski and P. Góras and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80054000861&partnerID=40&md5=1bfcff3f35cb27828448f3843de4eee3},

issn = {12301485},

year  = {2011},

date = {2011-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {20},

number = {3},

pages = {619-622},

abstract = {pH values of the bark of the common tree species black locust (Robinia pseudoacacia L.), sycamore maple (Acer pseudoplatanus L.), European yew (Taxus baccata L.), and European ash (Fraxinus excelsior L.) were evaluated to determine the acid gaseous pollution impact on their bark in eight heavily industrialized cities of southern Poland and relatively unpolluted areas of the Beskidy Mountains, Częstochowa Upland, and Nida Basin. It has been stated that the correlation between SOinf2/inf levels in the atmosphere and the reaction of tree bark exists in all investigated tree species. Hence, the reaction of the bark of these species could be used as a simple indicator of air pollution. The results suggest that the European ash bark could be the best bioindi-cator.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-80053987120,

title = {Effects of Zn, Cd, Pb on physiological response of silene vulgaris plants from selected populations},

author = { A. Nadgórska-Socha and M. Kandziora-Ciupa and R. Ciepał and K. Walasek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80053987120&partnerID=40&md5=9e57b72d916fb99986a2c11e2b050b6c},

issn = {12301485},

year  = {2011},

date = {2011-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {20},

number = {3},

pages = {599-604},

abstract = {In Silene vulgaris plants (Szopienice population; Heap population; Calamine population) grown on substrate with the addition of Cd, Zn, and Pb, accumulation of heavy metals and antioxidant stress parameters were compared. The highest accumulation of the studied elements was noticed in leaves of Heap population. There was also shown different sensitivity of antioxidant system in Silene vulgaris leaves in response to the studied heavy metals. The highest concentration of non protein thiols and proline was shown in the plant leaves of Calamine population grown on substrate with the addition of Cd and a mixture of metals. In the plant leaves of Szopienice population grown on substrate with the addition of Zn and the substrate with the mixture of metals in the case of Heap population plants, we noticed the growth of guaiacol peroxidase activity.},

note = {13},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-73149106985,

title = {The effects of Aphis fabae infestation on the antioxidant response and heavy metal content in field grown Philadelphus coronarius plants},

author = { A. Kafel and A. Nadgórska-Socha and J. Gospodarek and A. Babczyńska and M. Skowronek and M. Kandziora-Ciupa and K. Rozpędek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-73149106985&doi=10.1016%2fj.scitotenv.2009.11.013&partnerID=40&md5=f6dbe3421fa1628ef0488ad2a02e3aa3},

doi = {10.1016/j.scitotenv.2009.11.013},

issn = {00489697},

year  = {2010},

date = {2010-01-01},

journal = {Science of the Total Environment},

volume = {408},

number = {5},

pages = {1111-1119},

abstract = {The purpose of this study was to explore a possible relationship between the soil availability of metals and their concentrations in various parts of Philadelphus coronarius plants. Moreover, the possible impact of an aphid infestation on the contamination and antioxidant response of plants from the urban environment of Kraków and the reference rural area of Zagaje Stradowskie (southern Poland) was analyzed. The contents of the glutathione, proline, non-protein - SH groups, antioxidants, and phosphorous and the levels of guaiacol peroxidase and catalase activity in leaves and shoots either infested or not by the aphid Aphis fabae Scop., were measured. The potential bioavailability of metals (Cd; Cu; Ni; Pb; Zn) in the soil and their concentrations in P. coronarius plants originating from both sites were compared. The antioxidant responses were generally elevated in the plants in the polluted area. Such reactions were additionally changed by aphid infestation. Generally, the concentrations of metals in the HNO3 and CaCl2 extractants of the soils from two layers at the 0-20 and 20-40 cm depths from the polluted area were higher than in those from the reference area. Such differences were found for nickel and lead (in all examined extractants), zinc (in soil extractants from the layer at 20-40 cm) and cadmium (in HNO3 extractants). Significant positive relationships between the lead concentrations in the soil and in the plants were found. In the parts of plants from the polluted area, higher concentrations of Pb and Zn (leaves and shoots) and Cd (shoots) were recorded. The shoots and leaves of plants infested with aphids had higher concentrations of Zn but lower Pb. Moreover, their leaves had higher contaminations of Cu and Ni. In conclusion, aphids affected not only the antioxidant response of the plants but also their contamination with metals, especially contamination of the leaves. © 2009 Elsevier B.V. All rights reserved.},

note = {41},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77949441404,

title = {Comparison of the effect of liming and magnesium treatment of heavy metal contaminated soil on the content of magnesium, calcium and iron in broad beans (Vcia faba L. ssp. maior) [Porównanie oddziaływania wapnowania i nawożenia magnezowego gleby skażonej metalami ciężkimi na zawartość magnezu, wapnia i żelaza w roślinach bobu (Vicia faba L. ssp. maior)]},

author = { J. Gospodarek and A. Nadgórska-Socha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77949441404&doi=10.5601%2fjelem.2010.15.1.81-88&partnerID=40&md5=9c1f49a5591dfde3978752932b52e147},

doi = {10.5601/jelem.2010.15.1.81-88},

issn = {16442296},

year  = {2010},

date = {2010-01-01},

journal = {Journal of Elementology},

volume = {15},

number = {1},

pages = {81-88},

publisher = {Polish Society Magnesium Research},

abstract = {Tests have been conducted to determine the effect of liming and magnesium treatment on the content of magnesium, calcium and iron in broad bean plants growing on soil polluted with such heavy metals as cadmium, lead, nickel, copper and zinc. In 2005, an experiment was conducted in the village Zagaje Stradowskie (Świętokrzyskie Province) on degraded Chernozem formed from loess, acid in reaction and containing 1.13% of organic carbon. Analyses were performed on aerial parts of cv. White Windsor broad bean (Vicia faba L. ssp. maior), cultivated in three series: on limed soil, on soil receiving magnesium fertilizers; on unlimed soil without magnesium fertilization. In each se-ries, the plants were cultivated on the following objects: unpolluted soil with a natural content of heavy metals (control); unpolluted soil with a natural content of heavy metals and mineral fertilization (control+NPK); soil polluted with a cadmium dose 4 mg·kg-1 d.m.; soil polluted with a dose of 530 mg·kg-1 of lead; soil contaminated with a copper dose 85 mg·kg-1 d.m., soil contaminated with a dose of 1000 mg·kg-1 of zinc and soil polluted with a nickel dose 110 mg·kg-1 d.m. Liming was based on the analysis of hydrolytic acidity of soils from individual objects. The administered dose was established according to 1Hh. Magnesium treatments were identical in all objects. i.e. 20.4 mg·kg-1 soil d.m. Soil contamination with zinc or nickel leads to a considerable decrease in magnesium and calcium level in broad bean aerial parts but rises iron level. Liming rather than ma gnesium fertilization applied to soil polluted with heavy metals, such as zinc or nickel, contributes to balancing the content of the analyzed macronutrients in broad beans. The content of Ca, Fe and Mg in plants after liming approached the level determined in the control plants.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}