@article{2-s2.0-85184730898,

title = {Consumption of polypropylene caused some ultrastructural and physiological changes in some tissues of Galleria mellonella (Lepidoptera: Pyralidae) larvae},

author = { M.M. Rost-Roszkowska and P. Mermer and Ł. Chajec and A. Sosinka and G. Wilczek and S. Student and A.K. Wrońska and O. Karnówka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184730898&doi=10.1080%2f24750263.2024.2308529&partnerID=40&md5=bf8c4c030f37d08c900af092c647bfff},

doi = {10.1080/24750263.2024.2308529},

issn = {24750263},

year  = {2024},

date = {2024-01-01},

journal = {European Zoological Journal},

volume = {91},

number = {1},

pages = {213-234},

publisher = {Taylor and Francis Ltd.},

abstract = {G. mellonella is a promising species for use in the biodegradation of plastics. It is easy to breed and has high resistance to diverse climatic conditions, which is particularly valuable when considering its potential application in the decomposition of plastics. Thus, it demonstrated the capacity for biodegradation of the most common types of plastics, such as polyethylene (PE) and polypropylene (PP). However, reports on whether consumed plastics or their decomposition products will adversely affect the structure and functioning of the internal organs are rather poor. The studies aimed to determine whether the consumption of PP by a greater wax moth larvae caused any ultrastructural changes in the organs of the animal’s body, evaluate the survival rate of the animals, and describe their reproduction. Thus, this study provided an understanding of histological and ultrastructural changes caused, or not caused, by the PP diet. We investigated three organs–midgut, silk gland, and fat body–under PP consumption by G. mellonella caterpillars (7th instar larvae). The level of reactive oxygen species (ROS) in selected organs, as well as the ability of larvae to survive and undergo metamorphosis were also examined. The animals were divided into four groups: G0-C, G0-S, G0-24, and G0-48. The research used transmission electron microscopy (TEM), confocal microscopy, and flow cytometry. Our study showed that a diet containing PP did not affect internal organs at the ultrastructural level. Cells in the analyzed organs–midgut, silk gland, and fat body–showed no degenerative changes. An increase in the intensity of autophagy and cell vacuolization was noted, but they probably act as a survival pathway. These observations suggest that the final larval stage of the greater wax moth can potentially be applied in PP biodegradation. © 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85144323876,

title = {Effect of gluten on the digestive tract and fat body of Telodeinopus aoutii (Diplopoda)},

author = { F. Błaszczyk and A. Sosinka and G. Wilczek and S. Student and M.M. Rost-Roszkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144323876&doi=10.1002%2fjmor.21546&partnerID=40&md5=e081a74179e2400fb67b1e829e62bcc9},

doi = {10.1002/jmor.21546},

issn = {03622525},

year  = {2023},

date = {2023-01-01},

journal = {Journal of Morphology},

volume = {284},

number = {1},

publisher = {John Wiley and Sons Inc},

abstract = {Adult specimens or larvae of invertebrates used as food for vertebrates are often maintained close to gluten so they might become vectors for cereal proteins. However, the tissues and internal organs can respond differently in animals with different feeding habits. The midgut epithelium might be a first and sufficient barrier preventing uptake and effects of gluten on the whole body, while the fat body is the main organ that accumulates different xenobiotics. Good models for such research are animals that do not feed on gluten-rich products in their natural environment. The project's goal was to investigate alterations in the midgut epithelium and fat body of the herbivorous millipede Telodeinopus aoutii (Diplopoda) and analyze cell death processes activated by gluten. It enabled us to determine whether changes were intensified or reversed by adaptive mechanisms. Adult specimens were divided into control and experimental animals fed with mushrooms supplemented with gluten and analyzed using transmission electron microscopy, flow cytometry, and confocal microscopy. Two organs were isolated for the qualitative and quantitative analysis: the midgut and the fat body. Our study of the herbivorous T. aoutii which does not naturally feed on gluten containing diet showed that continuous and prolonged gluten feeding activates repair processes that inhibit the processes of cell death (apoptosis and necrosis) and induce an increase in cell viability. © 2022 Wiley Periodicals LLC.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85075281812,

title = {Enzymatic activities in the digestive tract of spirostreptid and spirobolid millipedes (Diplopoda: Spirostreptida and Spirobolida)},

author = { V. Šustr and S. Semanová and M.M. Rost-Roszkowska and K. Tajovský and A. Sosinka and F. Kaszuba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075281812&doi=10.1016%2fj.cbpb.2019.110388&partnerID=40&md5=81875be3727b690bdc14d9c10aaf86f8},

doi = {10.1016/j.cbpb.2019.110388},

issn = {10964959},

year  = {2020},

date = {2020-01-01},

journal = {Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology},

volume = {241},

publisher = {Elsevier Inc.},

abstract = {Millipedes represent a model for the study of organic matter transformation, animal-microbial interactions, and compartmentalisation of digestion. The activity of saccharidases (amylase; laminarinase; cellulase; xylanase; chitinase; maltase; cellobiase; and trehalase) and protease were measured in the midgut and hindgut contents and walls of the millipedes Archispirostreptus gigas and Epibolus pulchripes. Assays done at pH 4 and 7 confirmed activities of all enzymes except xylanase. Hydrolysing of starch and laminarin prevailed. The hindgut of E. pulchripes was shorter, less differentiated. Micro-apocrine secretion was observed only in the midgut of A. gigas. Merocrine secretion was present in midgut and hindgut of E. pulchripes, and in the pyloric valve and anterior hindgut of A. gigas. Alpha-polysaccharidases were mostly active in the midgut content and walls, with higher activity at pH 4. The low activity of amylase (A. gigas) and laminarinase (E. pulchripes) in midgut tissue may indicate their synthesis in salivary glands. Cellulases were found in midgut. Chitinases, found in midgut content and tissue (E. pulchripes) or concentrated in the midgut wall (A. gigas), were more active at an acidic pH. Polysaccharidases were low in hindguts. Protease shows midgut origin and alkaline activity extending to the hindgut in E. pulchripes, whereas in A. gigas it is of salivary gland origin and acid activity restricted to the midgut. Some disaccharidases, with more alkaline activity, showed less apparent midgut-hindgut differences. It may indicate an axial separating of the primary and secondary digestion along the intestinal pH gradient or the presence of enzymes of hindgut parasites. © 2019},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85065912638,

title = {Autophagy and apoptosis in the midgut epithelium of millipedes},

author = { M.M. Rost-Roszkowska and J. Vilimová and K. Tajovský and A. Chachulska-Żymełka and A. Sosinka and M. Kszuk-Jendrysik and A. Ostróżka and F. Kaszuba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065912638&doi=10.1017%2fS143192761900059X&partnerID=40&md5=c61361bfea2cb26d1ebf674d1ed0a10d},

doi = {10.1017/S143192761900059X},

issn = {14319276},

year  = {2019},

date = {2019-01-01},

journal = {Microscopy and Microanalysis},

volume = {25},

number = {4},

pages = {1004-1016},

publisher = {Cambridge University Press},

abstract = {The process of autophagy has been detected in the midgut epithelium of four millipede species: Julus scandinavius, Polyxenus lagurus, Archispirostreptus gigas, and Telodeinopus aoutii. It has been examined using transmission electron microscopy (TEM), which enabled differentiation of cells in the midgut epithelium, and some histochemical methods (light microscope and fluorescence microscope). While autophagy appeared in the cytoplasm of digestive, secretory, and regenerative cells in J. scandinavius and A. gigas, in the two other species, T. aoutii and P. lagurus, it was only detected in the digestive cells. Both types of macroautophagy, the selective and nonselective processes, are described using TEM. Phagophore formation appeared as the first step of autophagy. After its blind ends fusion, the autophagosomes were formed. The autophagosomes fused with lysosomes and were transformed into autolysosomes. As the final step of autophagy, the residual bodies were detected. Autophagic structures can be removed from the midgut epithelium via, e.g., atypical exocytosis. Additionally, in P. lagurus and J. scandinavius, it was observed as the neutralization of pathogens such as Rickettsia-like microorganisms. Autophagy and apoptosis ca be analyzed using TEM, while specific histochemical methods may confirm it. © Microscopy Society of America 2019.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85040594067,

title = {The ultrastructure of the hepatic cells in millipedes (Myriapoda, Diplopoda)},

author = { M.M. Rost-Roszkowska and J. Vilimová and K. Tajovský and V. Šustr and A. Sosinka and M. Kszuk-Jendrysik and A. Ostróżka and F. Kaszuba and K. Kamińska and A. Marchewka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040594067&doi=10.1016%2fj.jcz.2018.01.006&partnerID=40&md5=e1a5d1c546a04e9960db4d0b7878c65b},

doi = {10.1016/j.jcz.2018.01.006},

issn = {00445231},

year  = {2018},

date = {2018-01-01},

journal = {Zoologischer Anzeiger},

volume = {274},

pages = {95-102},

publisher = {Elsevier GmbH},

abstract = {The hepatic cells are characteristic cells that occur in millipede body where they form a continuous layer which surrounds the midgut epithelium. The following species, which represent five millipede orders were selected: Julus scandinavius (Julida), Polyxenus lagurus (Polyxenida), Polydesmus angustus (Polydesmida; Polydesmidae), Strongylosoma stigmatosum (Polydesmida; Paradoxosomatidae), Epibolus pulchripes (Spirobolida; Pachybolidae), and two species of the order Spirostreptida, Archispirostreptus gigas and Telodeinopus aoutii. The hepatic cells are absent in P. lagurus in which the midgut epithelium is surrounded by the visceral muscles. In the other species they were arranged around the midgut as coherent hepatic cells of mesenchymal character. Each hepatic cell possessed its own basal lamina and formed the cellular processes which protrude into the basal lamina of the midgut to make contact with midgut digestive cells. Accumulation of reserve material in different millipede taxa has been described with the special emphasis on the process of autophagy in the cytoplasm of the hepatic cells. The lack of hepatic cells may represent an ancestral condition within millipedes. © 2018 Elsevier GmbH},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84923205361,

title = {The ultrastructure of the midgut epithelium in millipedes (Myriapoda, Diplopoda)},

author = { A. Sosinka and M.M. Rost-Roszkowska and J. Vilimová and K. Tajovský and M. Kszuk-Jendrysik and Ł. Chajec and L. Sonakowska and K. Kamińska and M. Hyra and I. Poprawa},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923205361&doi=10.1016%2fj.asd.2014.06.005&partnerID=40&md5=031bc4c9e5c7c026a821e76da1ca47ce},

doi = {10.1016/j.asd.2014.06.005},

issn = {14678039},

year  = {2014},

date = {2014-01-01},

journal = {Arthropod Structure and Development},

volume = {43},

number = {5},

pages = {477-492},

publisher = {Elsevier Ltd},

abstract = {The midgut epithelia of the millipedes Polyxenus lagurus, Archispirostreptus gigas and Julus scandinavius were analyzed under light and transmission electron microscopies. In order to detect the proliferation of regenerative cells, labeling with BrdU and antibodies against phosphohistone H3 were employed. A tube-shaped midgut of three millipedes examined spreads along the entire length of the middle region of the body. The epithelium is composed of digestive, secretory and regenerative cells. The digestive cells are responsible for the accumulation of metals and the reserve material as well as the synthesis of substances, which are then secreted into the midgut lumen. The secretions are of three types - merocrine, apocrine and microapocrine. The oval or pear-like shaped secretory cells do not come into contact with the midgut lumen and represent the closed type of secretory cells. They possess many electron-dense granules (. J.scandinavius) or electron-dense granules and electron-lucent vesicles (. A.gigas; P.lagurus), which are accompanied by cisterns of the rough endoplasmic reticulum. The regenerative cells are distributed individually among the basal regions of the digestive cells. The proliferation and differentiation of regenerative cells into the digestive cells occurred in J.scandinavius and A.gigas, while these processes were not observed in P.lagurus. As a resultof the mitotic division of regenerative cells, one of the newly formed cells fulfills the role of a regenerative cell, while the second one differentiates into a digestive cell. We concluded that regenerative cells play the role of unipotent midgut stem cells. © 2014 Elsevier Ltd.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84862319702,

title = {Ultrastructure and regeneration of midgut epithelial cells in Lithobius forficatus (Chilopoda, Lithobiidae)},

author = { Ł. Chajec and M.M. Rost-Roszkowska and J. Vilimová and A. Sosinka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862319702&doi=10.1111%2fj.1744-7410.2012.00264.x&partnerID=40&md5=5abb432b567a5d771b5bf86d35719751},

doi = {10.1111/j.1744-7410.2012.00264.x},

issn = {10778306},

year  = {2012},

date = {2012-01-01},

journal = {Invertebrate Biology},

volume = {131},

number = {2},

pages = {119-132},

abstract = {Lithobius forficatus (Myriapoda; Chilopoda; Lithobiidae) is a widespread species of centipede that is common across Europe. Its midgut epithelial cells are an important line of defense against toxic substances that originate in food, such as pathogens and metals. Despite this important role, the biology of the midgut epithelium is not well known. Here we describe the ultrastructure of the midgut epithelium, as well as the replacement of degenerated midgut epithelial cells. The midgut epithelium of L. forficatus is composed of digestive, secretory, and regenerative cells. The cytoplasm of digestive cells shows regionalization in organelle distribution, which is consistent with the role of these cells in secretion of enzymes, absorption of nutrients, and accumulation of lipids and glycogen. Secretory cells, which do not reach the luminal surface of the midgut epithelium, possess numerous electron-dense and electron-lucent granules and may have an endocrine function. Hemidesmosomes anchor secretory cells to the basal lamina. Regenerative cells play the role of midgut stem cells, as they are able to proliferate and differentiate. Their proliferation occurs in a continuous manner, and their progeny differentiate only into digestive cells. The regeneration of secretory cells was not observed. Mitotic divisions of regenerative cells were confirmed using immunolabeling against BrdU and phosphohistone H3. Hemocytes associate with the midgut epithelium, accumulating between the visceral muscles and beneath the basal lamina of the midgut epithelium. Hemocytes also occur among the digestive cells of the midgut epithelium in animals infected with Rickettsia-like microorganisms. These hemocytes presumably have an immunoprotective function in the midgut. © 2012 The American Microscopical Society, Inc.},

note = {20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84860582843,

title = {The role of autophagy in the midgut epithelium of Eubranchipus grubii (Crustacea, Branchiopoda, Anostraca)},

author = { M.M. Rost-Roszkowska and J. Vilimová and A. Sosinka and J. Skudlik and E. Franzetti},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860582843&doi=10.1016%2fj.asd.2012.01.001&partnerID=40&md5=a1812dfbcd6ca9a872cda5d84aa9dbc6},

doi = {10.1016/j.asd.2012.01.001},

issn = {14678039},

year  = {2012},

date = {2012-01-01},

journal = {Arthropod Structure and Development},

volume = {41},

number = {3},

pages = {271-279},

abstract = {Eubranchipus grubii (Crustacea; Branchiopoda; Anostraca) is an omnivorous filter feeder whose life span lasts no more than 12 weeks. Adult males and females of E. grubii were used for ultrastructural studies of the midgut epithelium and an analysis of autophagy. The midgut epithelium is formed by columnar digestive cells and no regenerative cells were observed. A distinct regionalization in the distribution of organelles appears - basal, perinuclear and apical regions were distinguished. No differences in the ultrastructure of digestive cells were observed between males and females. Autophagic disintegration of organelles occurs throughout the midgut epithelium. Degenerated organelles accumulate in the neighborhood of Golgi complexes, and these complexes presumably take part in phagophore and autophagosome formation. In some cases, the phagophore also surrounds small autophagosomes, which had appeared earlier. Fusion of autophagosomes and lysosomes was not observed, but lysosomes are enclosed during autophagosome formation. Autophagosomes and autolysosomes are discharged into the midgut lumen due to apocrine secretion. Autophagy plays a role in cell survival by protecting the cell from cell death. © 2012 Elsevier Ltd.},

note = {30},

keywords = {},

pubstate = {published},

tppubtype = {article}

}