@article{2-s2.0-85137529859,

title = {ABP1–TMK auxin perception for global phosphorylation and auxin canalization},

author = { J. Friml and M. Gallei and Z. Gelová and A. Johnson and E. Mazur and A. Monzer and L. Rodriguez and M. Roosjen and I. Verstraeten and B.D. Živanović and M. Zou and L. Fiedler and C. Giannini and P. Grones and P. Grones and W.A. Kaufmann and A. Kuhn and A. Kuhn and M. Randuch and N. Rýdza and N. Rýdza and S. Tan and A.D. Teplova and T. Kinoshita and D. Weijers and H. Rakusová},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85137529859&doi=10.1038%2fs41586-022-05187-x&partnerID=40&md5=fc74dfc82435a395a98978ae188c27c2},

doi = {10.1038/s41586-022-05187-x},

issn = {00280836},

year  = {2022},

date = {2022-01-01},

journal = {Nature},

volume = {609},

number = {7927},

pages = {575-581},

publisher = {Nature Research},

abstract = {The phytohormone auxin triggers transcriptional reprogramming through a well-characterized perception machinery in the nucleus. By contrast, mechanisms that underlie fast effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation of proteins or auxin feedback on its transport, remain unclear1–3. Whether auxin-binding protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required for the auxin-induced ultrafast global phospho-response and for downstream processes that include the activation of H+-ATPase and accelerated cytoplasmic streaming. abp1 and tmk mutants cannot establish auxin-transporting channels and show defective auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that lacks the capacity to bind auxin is unable to complement these defects in abp1 mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface signalling, which mediates the global phospho-response and auxin canalization. © 2022, The Author(s), under exclusive licence to Springer Nature Limited.},

note = {35},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85087993003,

title = {Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization},

author = { J. Zhang and E. Mazur and J. Balla and M. Gallei and P. Kalousek and Z. Medveďová and Y. Li and Y. Wang and T. Prát and M. Vasileva and V. Reinöhl and S. Procházka and R. Halouzka and P. Tarkowski and C. Luschnig and P.B. Brewer and J. Friml},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087993003&doi=10.1038%2fs41467-020-17252-y&partnerID=40&md5=26b300195980305f74c77d5924189e4c},

doi = {10.1038/s41467-020-17252-y},

issn = {20411723},

year  = {2020},

date = {2020-01-01},

journal = {Nature Communications},

volume = {11},

number = {1},

publisher = {Nature Research},

abstract = {Directional transport of the phytohormone auxin is a versatile, plant-specific mechanism regulating many aspects of plant development. The recently identified plant hormones, strigolactones (SLs), are implicated in many plant traits; among others, they modify the phenotypic output of PIN-FORMED (PIN) auxin transporters for fine-tuning of growth and developmental responses. Here, we show in pea and Arabidopsis that SLs target processes dependent on the canalization of auxin flow, which involves auxin feedback on PIN subcellular distribution. D14 receptor- and MAX2 F-box-mediated SL signaling inhibits the formation of auxin-conducting channels after wounding or from artificial auxin sources, during vasculature de novo formation and regeneration. At the cellular level, SLs interfere with auxin effects on PIN polar targeting, constitutive PIN trafficking as well as clathrin-mediated endocytosis. Our results identify a non-transcriptional mechanism of SL action, uncoupling auxin feedback on PIN polarity and trafficking, thereby regulating vascular tissue formation and regeneration. © 2020, The Author(s).},

note = {30},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85094936069,

title = {Receptor kinase module targets PIN-dependent auxin transport during canalization},

author = { J. Hajný and T. Prát and N. Rydza and L. Rodriguez and S. Tan and I. Verstraeten and D. Domjan and E. Mazur and E. Smakowska-Luzan and W. Smet and E. Mor and J. Nolf and B. Yang and W. Grunewald and G. Molnár and Y. Belkhadir and B. De Rybel and J. Friml},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85094936069&doi=10.1126%2fSCIENCE.ABA3178&partnerID=40&md5=7bc3e151f57ac44c7676d3ef25ae09a8},

doi = {10.1126/SCIENCE.ABA3178},

issn = {00368075},

year  = {2020},

date = {2020-01-01},

journal = {Science},

volume = {370},

number = {6516},

pages = {550-557},

publisher = {American Association for the Advancement of Science},

abstract = {Spontaneously arising channels that transport the phytohormone auxin provide positional cues for selforganizing aspects of plant development such as flexible vasculature regeneration or its patterning during leaf venation. The auxin canalization hypothesis proposes a feedback between auxin signaling and transport as the underlying mechanism, but molecular players await discovery. We identified part of the machinery that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like kinase) interact with and phosphorylate PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization, which macroscopically manifests as defects in leaf venation and vasculature regeneration after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback that coordinates polarization of individual cells during auxin canalization. © 2020 American Association for the Advancement of Science. All rights reserved.},

note = {28},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85085764194,

title = {Auxin canalization and vascular tissue formation by TIR1/AFB-mediated auxin signaling in Arabidopsis},

author = { E. Mazur and I. Kulik and J. Hajný and J. Friml},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085764194&doi=10.1111%2fnph.16446&partnerID=40&md5=730df5038e134be0a3606df0d73ce723},

doi = {10.1111/nph.16446},

issn = {0028646X},

year  = {2020},

date = {2020-01-01},

journal = {New Phytologist},

volume = {226},

number = {5},

pages = {1375-1383},

publisher = {Blackwell Publishing Ltd},

abstract = {Plant survival depends on vascular tissues, which originate in a self-organizing manner as strands of cells co-directionally transporting the plant hormone auxin. The latter phenomenon (also known as auxin canalization) is classically hypothesized to be regulated by auxin itself via the effect of this hormone on the polarity of its own intercellular transport. Correlative observations supported this concept, but molecular insights remain limited. In the current study, we established an experimental system based on the model Arabidopsis thaliana, which exhibits auxin transport channels and formation of vasculature strands in response to local auxin application. Our methodology permits the genetic analysis of auxin canalization under controllable experimental conditions. By utilizing this opportunity, we confirmed the dependence of auxin canalization on a PIN-dependent auxin transport and nuclear, TIR1/AFB-mediated auxin signaling. We also show that leaf venation and auxin-mediated PIN repolarization in the root require TIR1/AFB signaling. Further studies based on this experimental system are likely to yield better understanding of the mechanisms underlying auxin transport polarization in other developmental contexts. © 2020 The Authors. New Phytologist © 2020 New Phytologist Trust},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85078623144,

title = {Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis},

author = { E. Mazur and M. Gallei and M. Adamowski and H. Han and H.S. Robert and J. Friml},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078623144&doi=10.1016%2fj.plantsci.2020.110414&partnerID=40&md5=5c3f1a5a2c0a2d1ffffcad0ae5e48fc1},

doi = {10.1016/j.plantsci.2020.110414},

issn = {01689452},

year  = {2020},

date = {2020-01-01},

journal = {Plant Science},

volume = {293},

publisher = {Elsevier Ireland Ltd},

abstract = {The flexible development of plants is characterized by a high capacity for post-embryonic organ formation and tissue regeneration, processes, which require tightly regulated intercellular communication and coordinated tissue (re-)polarization. The phytohormone auxin, the main driver for these processes, is able to establish polarized auxin transport channels, which are characterized by the expression and polar, subcellular localization of the PIN1 auxin transport proteins. These channels are demarcating the position of future vascular strands necessary for organ formation and tissue regeneration. Major progress has been made in the last years to understand how PINs can change their polarity in different contexts and thus guide auxin flow through the plant. However, it still remains elusive how auxin mediates the establishment of auxin conducting channels and the formation of vascular tissue and which cellular processes are involved. By the means of sophisticated regeneration experiments combined with local auxin applications in Arabidopsis thaliana inflorescence stems we show that (i) PIN subcellular dynamics, (ii) PIN internalization by clathrin-mediated trafficking and (iii) an intact actin cytoskeleton required for post-endocytic trafficking are indispensable for auxin channel formation, de novo vascular formation and vascular regeneration after wounding. These observations provide novel insights into cellular mechanism of coordinated tissue polarization during auxin canalization. © 2020 The Authors},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84988908357,

title = {Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis},

author = { E. Mazur and E. Benková and J. Friml},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84988908357&doi=10.1038%2fsrep33754&partnerID=40&md5=80e07d5850ffad393dcd4f2b04e7b9bb},

doi = {10.1038/srep33754},

issn = {20452322},

year  = {2016},

date = {2016-01-01},

journal = {Scientific Reports},

volume = {6},

publisher = {Nature Publishing Group},

abstract = {Synchronized tissue polarization during regeneration or de novo vascular tissue formation is a plant-specific example of intercellular communication and coordinated development. According to the canalization hypothesis, the plant hormone auxin serves as polarizing signal that mediates directional channel formation underlying the spatio-temporal vasculature patterning. A necessary part of canalization is a positive feedback between auxin signaling and polarity of the intercellular auxin flow. The cellular and molecular mechanisms of this process are still poorly understood, not the least, because of a lack of a suitable model system. We show that the main genetic model plant, Arabidopsis (Arabidopsis thaliana) can be used to study the canalization during vascular cambium regeneration and new vasculature formation. We monitored localized auxin responses, directional auxin-transport channels formation, and establishment of new vascular cambium polarity during regenerative processes after stem wounding. The increased auxin response above and around the wound preceded the formation of PIN1 auxin transporter-marked channels from the primarily homogenous tissue and the transient, gradual changes in PIN1 localization preceded the polarity of newly formed vascular tissue. Thus, Arabidopsis is a useful model for studies of coordinated tissue polarization and vasculature formation after wounding allowing for genetic and mechanistic dissection of the canalization hypothesis. © The Author(s) 2016.},

note = {41},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84905679687,

title = {Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis},

author = { E. Mazur and E.U. Kurczyńska and J. Friml},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84905679687&doi=10.1007%2fs00709-014-0620-5&partnerID=40&md5=b9a414a86da0390d597a07374c3a6ec4},

doi = {10.1007/s00709-014-0620-5},

issn = {0033183X},

year  = {2014},

date = {2014-01-01},

journal = {Protoplasma},

volume = {251},

number = {5},

pages = {1125-1139},

publisher = {Springer-Verlag Wien},

abstract = {Development of cambium and its activity is important for our knowledge of the mechanism of secondary growth. Arabidopsis thaliana emerges as a good model plant for such a kind of study. Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC). These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions. Distribution of auxin was observed to be higher in differentiating into cambium parenchyma cells compared to cells within the pith and cortex. Expression of PIN1 in IPC was always preceded by auxin accumulation. Basal localization of PIN1 was already established in the cells prior to their periclinal division. These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles. The final consequence of which was the closure of the cambial ring within the stem. Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs). In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis. © 2014 Springer-Verlag Wien.},

note = {21},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84855334890,

title = {Rays, intrusive growth, and storied cambium in the inflorescence stems of Arabidopsis thaliana (L.) Heynh},

author = { E. Mazur and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855334890&doi=10.1007%2fs00709-011-0266-5&partnerID=40&md5=c34e5fceb33acc503bd926c670d8bf8b},

doi = {10.1007/s00709-011-0266-5},

issn = {0033183X},

year  = {2012},

date = {2012-01-01},

journal = {Protoplasma},

volume = {249},

number = {1},

pages = {217-220},

abstract = {Arabidopsis thaliana is a model plant used in analysis of different aspects of plant growth and development. Under suitable conditions, secondary growth takes place in the hypocotyl of Arabidopsis plants, a finding which helps in understanding many aspects of xylogenesis. However, not all developmental processes of secondary tissue can be studied here, as no secondary rays and intrusive growth have been detected in hypocotyl. However, results presented here concerning the secondary growth in inflorescence stems of Arabidopsis shows that both secondary rays and intrusive growth of cambial cells can be detected, and that, in the interfascicular regions, a storied cambium can be developed. © 2011 The Author(s).},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34447121906,

title = {Histological analysis of direct somatic embryogenesis in Arabidopsis thaliana (L.) Heynh},

author = { E.U. Kurczyńska and M.D. Gaj and A. Ujczak and E. Mazur},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34447121906&doi=10.1007%2fs00425-007-0510-6&partnerID=40&md5=3d00a2a2cebccc7fda9ee11b0da3e024},

doi = {10.1007/s00425-007-0510-6},

issn = {00320935},

year  = {2007},

date = {2007-01-01},

journal = {Planta},

volume = {226},

number = {3},

pages = {619-628},

abstract = {In Arabidopsis the in vitro culture of immature zygotic embryos (IZEs) at a late stage of development, on the solid medium containing synthetic auxin, leads to formation of somatic embryos via direct somatic embryogenesis (DSE). The presented results provide evidence that in IZE cells competent for DSE are located in the protodermis and subprotodermis of the adaxial side of cotyledons and somatic embryos displayed a single- or multicellular origin. Transgenic Arabidopsis lines expressing the GUS reporter gene, driven by the DR5 and LEC2 promoters, were used to analyse the distribution of auxin to mark embryogenic cells in cultured explants and develop somatic embryos. The analysis showed that at the start of the culture auxin was accumulated in all explant tissues, but from the fourth day onwards its location shifted to the protodermis and subprotodermis of the explant cotyledons. In globular somatic embryos auxin was detected in all cells, with a higher concentration in the protodermis, and in the heart stage its activity was mainly displayed in the shoot, root pole and cotyledon primordia. The embryogenic nature of dividing protodermal and subprotodermal cells accumulating auxin was confirmed by high expression of promoter activity of LEC2 in these cells. Analysis of symplasmic tracer (CFDA) distribution indicated symplasmic isolation between tissues engaged in DSE and other parts of an explant. Symplasmic isolation of somatic embryos from the explant was also detected. © 2007 Springer-Verlag.},

note = {93},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0036586682,

title = {Formation of spiral grain in the wood of Pinus sylvestris L.},

author = { W. Włoch and E. Mazur and M. Bełtowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036586682&doi=10.1007%2fs00468-002-0174-6&partnerID=40&md5=183632494d4bfe0a24fa62df42071e35},

doi = {10.1007/s00468-002-0174-6},

issn = {09311890},

year  = {2002},

date = {2002-01-01},

journal = {Trees - Structure and Function},

volume = {16},

number = {4-5},

pages = {306-312},

abstract = {In cambium producing wood of increasing spirality, the angle of inclination of fusiform cells to the stem axis is augmented with time. Frequently, events such as pseudotransverse anticlinal divisions and intrusive growth between radial walls do not explain the relatively large rate of change in the angle. The present analysis of the cambium of Pinus sylvestris L. indicates that the large rate of change in the angle of a cell inclination is caused by oriented intrusive growth of initial cells in certain files, entering the space between the tangential walls of neighbouring files. Such intrusive growth of the initial cell ends is caused by a deflection of the ends of neighbouring initial cells in the radial direction. A periclinal division plane does not reach the deflected end. This results in two derivatives, unequal in size, the shorter of which remains an initial cell, whereas the longer becomes a xylem or phloem mother cell. When these events are intensive, they cause a rapid change of cell inclination along the stem axis. Such a rapid change can take place even without oblique anticlinal divisions.},

note = {23},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0035669845,

title = {Intensive change of inclination of cambial initials in Picea abies (L.) Karst. tumours},

author = { W. Włoch and E. Mazur and P. Kojs},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035669845&doi=10.1007%2fs00468-001-0127-5&partnerID=40&md5=56624007abde40a56c9435c40d5644fe},

doi = {10.1007/s00468-001-0127-5},

issn = {09311890},

year  = {2001},

date = {2001-01-01},

journal = {Trees - Structure and Function},

volume = {15},

number = {8},

pages = {498-502},

abstract = {Changes in the inclination of cambial fusiform cells producing wavy grain wood in spruce trunk tumours were investigated. The rate of changes of cell inclination depends on events such as intrusive growth between periclinal walls of cells of neighbouring files and unequal periclinal divisions. Intrusive growth of initial cell ends between radial walls does not affect the change in cell inclination. The only reason for such change is the intrusive growth of initial cell ends between periclinal cell walls of neighbouring files. Initial cells do not form a continuous, integral layer. After periclinal division of initial cells the status of the initial is defined by the initial surface. After periclinal divisions of mother cells packets of cells are produced. In cambium, which does not rearrange, packets of cells originating from the same initial cell are located in one file. Cells in these packets do not usually grow intrusively and do not change their position. In tumorous cambium, packets are dislocated in periclinal planes. In the tangential surface a very large and rapid change in inclination of fusiform cells was observed. In spite of this change, which causes heterogeneous files, the order (arrangement) of the files was not disturbed.},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}