@article{2-s2.0-85185955844,

title = {Concentration- and Time-Dependent Dietary Exposure to Graphene Oxide and Silver Nanoparticles: Effects on Food Consumption and Assimilation, Digestive Enzyme Activities, and Body Mass in Acheta domesticus},

author = { R. Seyed Alian and B. Flasz and A. Kędziorski and Ł. Majchrzycki and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185955844&doi=10.3390%2finsects15020089&partnerID=40&md5=5297f84c7a1e136e6fa7655512ea890c},

doi = {10.3390/insects15020089},

issn = {20754450},

year  = {2024},

date = {2024-01-01},

journal = {Insects},

volume = {15},

number = {2},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {The advancement of nanotechnology poses a real risk of insect exposure to nanoparticles (NPs) that can enter the digestive system through contaminated food or nanopesticides. This study examines whether the exposure of model insect species—Acheta domesticus—to increasing graphene oxide (GO) and silver nanoparticle (AgNP) concentrations (2; 20; and 200 ppm and 4; 40; and 400 ppm; respectively) could change its digestive functions: enzymes’ activities, food consumption, and assimilation. We noticed more pronounced alterations following exposure to AgNPs than to GO. They included increased activity of α-amylase, α-glucosidase, and lipase but inhibited protease activity. Prolonged exposure to higher concentrations of AgNPs resulted in a significantly decreased food consumption and changed assimilation compared with the control in adult crickets. A increase in body weight was observed in the insects from the Ag4 group and a decrease in body weight or no effects were observed in crickets from the Ag40 and Ag400 groups (i.e.; 4; 40; or 400 ppm of AgNPs; respectively), suggesting that even a moderate disturbance in nutrient and energy availability may affect the body weight of an organism and its overall condition. This study underscores the intricate interplay between NPs and digestive enzymes, emphasizing the need for further investigation to comprehend the underlying mechanisms and consequences of these interactions. © 2024 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85178151628,

title = {Survival, growth and digestive functions after exposure to nanodiamonds - Transgenerational effects beyond contact time in house cricket strains},

author = { M. Augustyniak and A.K. Ajay and A. Kędziorski and M. Tarnawska and M.M. Rost-Roszkowska and B. Flasz and A. Babczyńska and B. Mazur and K. Rozpędek and R.S. Alian and M. Skowronek and E. Świerczek and Kl. Wiśniewska and P. Ziętara},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85178151628&doi=10.1016%2fj.chemosphere.2023.140809&partnerID=40&md5=8596e027ee47c37b61120de37d91f704},

doi = {10.1016/j.chemosphere.2023.140809},

issn = {00456535},

year  = {2024},

date = {2024-01-01},

journal = {Chemosphere},

volume = {349},

publisher = {Elsevier Ltd},

abstract = {The long-term exposure effects of nanodiamonds (NDs), spanning an organism's entire lifespan and continuing for subsequent generation, remain understudied. Most research has focused on evaluating their biological impacts on cell lines and selected organisms, typically over short exposure durations lasting hours or days. The study aimed to assess growth, mortality, and digestive functions in wild (H) and long-lived (D) strains of Acheta domesticus (Insecta: Orthoptera) after two-generational exposure to NDs in concentrations of 0.2 or 2 mg kg−1 of food, followed by their elimination in the third generation. NDs induced subtle stimulating effect that depended on the strain and generation. In the first generation, more such responses occurred in the H than in the D strain. In the first generation of H strain insects, contact with NDs increased survival, stimulated the growth of young larvae, and the activity of most digestive enzymes in mature adults. The same doses and exposure time did not cause similar effects in the D strain. In the first generation of D strain insects, survival and growth were unaffected by NDs, whereas, in the second generation, significant stimulation of those parameters was visible. Selection towards longevity appears to support higher resistance of the insects to exposure to additional stressor, at least in the first generation. The cessation of ND exposure in the third generation caused potentially harmful changes, which included, e.g., decreased survival probability in H strain insects, slowed growth of both strains, as well as changes in heterochromatin density and distribution in nuclei of the gut cells in both strains. Such a reaction may suggest the involvement of epigenetic inheritance mechanisms, which may become inadequate after the stress factor is removed. © 2023 Elsevier Ltd},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85169134510,

title = {Multigenerational Effects of Graphene Oxide Nanoparticles on Acheta domesticus DNA Stability},

author = { B. Flasz and A.K. Ajay and M. Tarnawska and A. Babczyńska and Ł. Majchrzycki and A. Kędziorski and Ł. Napora-Rutkowski and E. Świerczek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85169134510&doi=10.3390%2fijms241612826&partnerID=40&md5=94952f38d3a2995d15e764932e1dfb79},

doi = {10.3390/ijms241612826},

issn = {16616596},

year  = {2023},

date = {2023-01-01},

journal = {International Journal of Molecular Sciences},

volume = {24},

number = {16},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {The use of nanoparticles like graphene oxide (GO) in nanocomposite industries is growing very fast. There is a strong concern that GO can enter the environment and become nanopollutatnt. Environmental pollutants’ exposure usually relates to low concentrations but may last for a long time and impact following generations. Attention should be paid to the effects of nanoparticles, especially on the DNA stability passed on to the offspring. We investigated the multigenerational effects on two strains (wild and long-lived) of house cricket intoxicated with low GO concentrations over five generations, followed by one recovery generation. Our investigation focused on oxidative stress parameters, specifically AP sites (apurinic/apyrimidinic sites) and 8-OHdG (8-hydroxy-2′-deoxyguanosine), and examined the global DNA methylation pattern. Five intoxicated generations were able to overcome the oxidative stress, showing that relatively low doses of GO have a moderate effect on the house cricket (8-OHdG and AP sites). The last recovery generation that experienced a transition from contaminated to uncontaminated food presented greater DNA damage. The pattern of DNA methylation was comparable in every generation, suggesting that other epigenetic mechanisms might be involved. © 2023 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85145976280,

title = {Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus},

author = { B. Flasz and M. Dziewięcka and A.K. Ajay and M. Tarnawska and A. Babczyńska and A. Kędziorski and Ł. Napora-Rutkowski and P. Ziętara and E. Świerczek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145976280&doi=10.3390%2fijms24010290&partnerID=40&md5=5d497b4e9e8b4082341d87d5be39d3d6},

doi = {10.3390/ijms24010290},

issn = {16616596},

year  = {2023},

date = {2023-01-01},

journal = {International Journal of Molecular Sciences},

volume = {24},

number = {1},

publisher = {MDPI},

abstract = {The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage; double-strand breaks—DSB; 8-hydroxy-2′-deoxyguanosine-8-OHdG; abasic site—AP sites), DNA damage response parameters, and global methylation in the model organism Acheta domesticus. Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms. © 2022 by the authors.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85131527033,

title = {Does age pay off? Effects of three-generational experiments of nanodiamond exposure and withdrawal in wild and longevity-selected model animals},

author = { M. Augustyniak and A. Babczyńska and M. Dziewięcka and B. Flasz and J. Karpeta-Kaczmarek and A. Kędziorski and B. Mazur and K. Rozpędek and R. Seyed Alian and M. Skowronek and E. Świerczek and A. Świętek and M. Tarnawska and Kl. Wiśniewska and P. Ziętara},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131527033&doi=10.1016%2fj.chemosphere.2022.135129&partnerID=40&md5=a9ef4d24a7c7b03b85af2b6ebf7bc9e4},

doi = {10.1016/j.chemosphere.2022.135129},

issn = {00456535},

year  = {2022},

date = {2022-01-01},

journal = {Chemosphere},

volume = {303},

publisher = {Elsevier Ltd},

abstract = {Nanodiamonds (NDs) are considered a material with low toxicity. However, no studies describe the effects of ND withdrawal after multigenerational exposure. The aim was to evaluate ND exposure (in the 1st and 2nd generations) effects at low concentrations (0.2 or 2 mg kg−1) and withdrawal (in the 3rd generation) in the wild (H) and longevity-selected (D) model insect Acheta domesticus. We measured selected oxidative stress parameters, immunity, types of cell death, and DNA damage. Most of the results obtained in the 1st generation, e.g., catalase (CAT), total antioxidant capacity (TAC), heat shock proteins (HSP70), defensins, or apoptosis level, confirmed no significant toxicity of low doses of NDs. Interestingly, strain-specific differences were observed. D-strain crickets reduced autophagy, the number of ROS+ cells, and DNA damage. The effect can be a symptom of mobilization of the organism and stimulation of physiological defense mechanisms in long-living organisms. The 2nd-generation D-strain insects fed ND-spiked food at higher concentrations manifested a reduction in CAT, TAC, early apoptosis, and DNA damage, together with an increase in HSP70 and defensins. ROS+ cells and cells with reduced membrane potential and autophagy did not differ significantly from the control. H-strain insects revealed a higher number of ROS+ cells and cells with reduced membrane potential, decreased CAT activity, and early apoptosis. Elimination of NDs from the diet in the 3rd generation did not cause full recovery of the measured parameters. We noticed an increase in the concentration of HSP70 and defensins (H-strain) and a decrease in apoptosis (D-strain). However, the most visible increase was a significant increase in DNA damage, especially in H-strain individuals. The results suggest prolonged adverse effects of NDs on cellular functions, reaching beyond “contact time” with these particles. Unintentional and/or uncontrolled ND pollution of the environment poses a new challenge for all organisms inhabiting it, particularly during multigenerational exposure. © 2022 The Authors},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85112577869,

title = {Multigenerational selection towards longevity changes the protective role of vitamin C against graphene oxide-induced oxidative stress in house crickets},

author = { B. Flasz and M. Dziewięcka and A. Kędziorski and M. Tarnawska and J. Augustyniak and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85112577869&doi=10.1016%2fj.envpol.2021.117996&partnerID=40&md5=7ef2db048ee15bffac23fcd838fd4ba7},

doi = {10.1016/j.envpol.2021.117996},

issn = {02697491},

year  = {2021},

date = {2021-01-01},

journal = {Environmental Pollution},

volume = {290},

publisher = {Elsevier Ltd},

abstract = {This research was designed to investigate changes that can arise in an invertebrate organism due to stress caused by a strong prooxidant, graphene oxide (GO), and a potent antioxidant, vitamin C. The study aimed to investigate if vitamin C may support convalescence after chronic GO intoxication. We investigated the toxicity of chronic dietary graphene oxide administration in house cricket (Acheta domesticus) types: wild and selected for longevity (with a better developed antioxidant system; conducive to long life). Vitamin C was applied immediately after cessation of graphene oxide intoxication to check if it can support the remedial effect. The condition of cells, DNA stability, catalase activity, and the reproduction potential, measured as the Vitellogenin (Vg) protein expression level, were investigated in control and GO treated groups, recovery groups (-GO), and recovery groups with Vit. C (-GO + Vit.C). In this study vitamin C had no evident remedial effect on the house crickets exposed to graphene oxide. Most probably, the mechanism of vitamin C action, in case of intoxication with nanoparticles, is much more complicated. In the context of the results obtained, it is worth considering whether Vit. C, applied after GO intoxication, causes further disturbance of homeostasis in terms of the cells' redox potential. © 2021 Elsevier Ltd},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85105467182,

title = {Multigenerational graphene oxide intoxication results in reproduction disorders at the molecular level of vitellogenin protein expression in Acheta domesticus},

author = { B. Flasz and M. Dziewięcka and A. Kędziorski and M. Tarnawska and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105467182&doi=10.1016%2fj.chemosphere.2021.130772&partnerID=40&md5=412a7ee17abcd00614fdde8751e5fd1d},

doi = {10.1016/j.chemosphere.2021.130772},

issn = {00456535},

year  = {2021},

date = {2021-01-01},

journal = {Chemosphere},

volume = {280},

publisher = {Elsevier Ltd},

abstract = {The anthropogenic activities may lead to accumulation of graphene oxide (GO) pollution in the environment. Organisms exposed to chronic or multigenerational GO intoxication can present reproduction depletion. Vitellogenin (Vg) has been used as a parameter for evaluating female fertility due to its importance in embryo nutrition. In this study, we used a promising model organism, Acheta domesticus, which was intoxicated with GO in food for three generations. The aim of the study was to investigate the process of Vg synthesis in crickets depending on the exposure time, GO concentration, and age of the females. The results revealed that chronic GO intoxication had adverse effects on the Vg expression pattern. The 1st generation of insects showing low Vg expression was most affected. The 2nd generation of A. domesticus presented a high Vg expression. The last investigated generation seemed to cope with stress caused by GO, and the Vg expression was balanced. We suggest that the epigenetic mechanisms may play a role in the information transfer to the next generations on how to react to the risk factor and keep reproduction at a high rate. We suspect that chronic GO intoxication can disturb the regular formation of the Vg quaternary structure, resulting in consequences for developing an embryo. © 2021 Elsevier Ltd},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85104957867,

title = {Towards understanding Cameraria ohridella (Lepidoptera: Gracillariidae) development: effects of microhabitat variability in naturally growing horse-chestnut tree canopy},

author = { P. Łaszczyca and M. Nakonieczny and A. Kędziorski and A. Babczyńska and M. Wiesner},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104957867&doi=10.1007%2fs00484-021-02119-8&partnerID=40&md5=2b01bb6369bde5c53f17613274c9d44c},

doi = {10.1007/s00484-021-02119-8},

issn = {00207128},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Biometeorology},

volume = {65},

number = {10},

pages = {1647-1658},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {Dwelling intensity of horse-chestnut miner (Cameraria ohridella) larvae in various leaves insolation and temperature was measured to determine whether this pest’s development follows a predictable pattern or depends more on local microenvironment conditions. Mines growing on leaves of mature host plants (Aesculus hippocastanum L.) in their natural conditions were photographed for two consecutive generations of the pest and in two separated vegetation periods. Apart from meteorological data obtained from the nearest station, the temperature of intact and mined parts of sun-exposed and shaded leaf blades was measured at various daytimes throughout the experiment. Obtained sets of digital data were analysed and combined to model mine area growth as a function of degree-days sum by adopting of Verhulst logistic equation. We showed the predictive potential of our model based on experimental data, and it may be useful in the scheduling of pest control measures in natural conditions. Our analyses also revealed that despite significant differences in microenvironment conditions depending on mines’ insolation, the horse-chestnut miner larvae could partially compensate for them and complete their development at similar endpoints expressed as the cumulative sum of degree-days. We conclude that computer-aided analysis of photographic documentation of leaf-miner larval growth followed by mathematical modelling offers a noninvasive, reliable, and inexpensive alternative for monitoring local leaf-miners populations. © 2021, The Author(s).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85106267328,

title = {Do nanoparticles cause hormesis? Early physiological compensatory response in house crickets to a dietary admixture of GO, Ag, and GOAg composite},

author = { R. Seyed Alian and M. Dziewięcka and A. Kędziorski and Ł. Majchrzycki and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106267328&doi=10.1016%2fj.scitotenv.2021.147801&partnerID=40&md5=1329bc1fff0f8d3dd59ba7eee545145c},

doi = {10.1016/j.scitotenv.2021.147801},

issn = {00489697},

year  = {2021},

date = {2021-01-01},

journal = {Science of the Total Environment},

volume = {788},

publisher = {Elsevier B.V.},

abstract = {This study aimed to identify the physiological responses of house cricket females following short-term exposure to relatively low dietary doses of graphene oxide (GO; 20 μg · g−1 food), silver (Ag; 400 μg · g−1 food) nanoparticles (NPs), or graphene oxide‑silver nanoparticle composite (GO-AgNPs; 20: 400 μg · g−1 food). Energy intake and distribution were measured on the third, sixth, and tenth day. A semi-quantitative API®ZYM assay of digestive enzyme fingerprints was performed on the third and tenth day of continuous treatment. Physicochemical properties of the NPs were obtained by combining SEM, EDX spectrometry, AFM, and DLS techniques. The obtained results showed decreased energy consumption, particularly assimilation as an early response to dietary NPs followed by compensatory changes in feeding activity leading to the same consumption and assimilation throughout the experimental period (10 days). The increased activities of digestive enzymes in NP-treated females compared to the control on the third day of the experiment suggest the onset of compensatory reactions of the day. Moreover, the insects treated with GO-AgNP composite retained more body water, suggesting increased uptake. The observed changes in the measured physiological parameters after exposure to NPs are discussed in light of hormesis. © 2021 The Authors},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85086727685,

title = {Vitellogenin expression, DNA damage, health status of cells and catalase activity in Acheta domesticus selected according to their longevity after graphene oxide treatment},

author = { B. Flasz and M. Dziewięcka and A. Kędziorski and M. Tarnawska and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086727685&doi=10.1016%2fj.scitotenv.2020.140274&partnerID=40&md5=0dbe9ad21f1a92fd747f33cc48150904},

doi = {10.1016/j.scitotenv.2020.140274},

issn = {00489697},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {737},

publisher = {Elsevier B.V.},

abstract = {The increased use of graphene oxide (GO) raises worrisome questions regarding its possible threat to various ecosystems. Invertebrates represent valuable organisms for environmental studies. The lifespan can influence the ability to cope with toxins, especially those that act via oxidative stress. Two strains of Acheta domesticus, which are selected for longevity, were tested. The main aim was to investigate how GO, when administrated in food, affects: the condition of cells, DNA stability, ROS generation and the reproduction potential (the Vitellogenin (Vg) protein expression). The “recovery effect” – after removing GO from the diet for 15 days – was also measured. The results revealed different responses to GO in the wild (H) and long-living (D) strains. The D strain had a higher catalase activity compared to the H strain on the 25th day of the imago stage. Removing GO from the food resulted in a decrease in the catalase activity to the level of the control. On the 5th day of the imago stage, the H strain had a higher cell mortality than the D strain in the GO-intoxicated groups. There was more DNA damage in the H strain compared to the long-living strain. A remedial effect was seen after the GO was removed from the diet. The total Vg protein expression was higher in the H strain and lower in the D strain. The results indicated a GO concentration-dependent outcome. In both strains, removing the GO from the food led to a high Vg expression. The Vg expression after GO treatment, particularly translation and post-translational processing, should be studied in detail in the future. The D strain of crickets had more specialized mechanisms for maintaining homeostasis than the H strain. Organisms can fight off negative effects of GO, especially when they have systems that are well developed against oxidative stress. © 2020 Elsevier B.V.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85083788720,

title = {Graphene oxide as a new anthropogenic stress factor - multigenerational study at the molecular, cellular, individual and population level of Acheta domesticus},

author = { M. Dziewięcka and B. Flasz and M.M. Rost-Roszkowska and A. Kędziorski and A. Kochanowicz and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85083788720&doi=10.1016%2fj.jhazmat.2020.122775&partnerID=40&md5=4c5ac8dd6643714067dd963491c30c94},

doi = {10.1016/j.jhazmat.2020.122775},

issn = {03043894},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Hazardous Materials},

volume = {396},

publisher = {Elsevier B.V.},

abstract = {Although interest in transgenerational phenomena is constantly growing, little is known about the long-term toxicity of nanoparticles. In this study we investigate the multigenerational effects of graphene oxide (GO) which was given to Acheta domesticus in low doses (0.2; 2 and 20 μg·g-1 of food) for three subsequent generations. We assessed the influence of GO nanoparticles in many contexts, basing on parameters which represented different levels of biological organization: activity of antioxidant enzymes, level of apoptosis, DNA damage, histological analysis, hatching abilities, body mass and body length of insects, as well as their survival rate. The results have shown that exposing insects to nanoparticles over an extended period of time causes surprising intergenerational effects, based on significant differences in the life cycle and reproductive processes, which are not always dose-dependent. The second generation of insects appeared as the most unstable among the parameters that were studied, and did not match trends and patterns in the first and third generation categories. An increase of DNA damage was observed, but only in the third generation. This reduction of genome stability can be perceived as an essential element of adaptation, leading to an increase of genotype variants, which then undergo selection. © 2020 Elsevier B.V.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85057501676,

title = {Evaluation of selected biological properties of the hunting web spider (Steatoda grossa, Theridiidae) in the aspect of short- and long-term exposure to cadmium},

author = { G. Wilczek and J. Karcz and M.M. Rost-Roszkowska and A. Kędziorski and P. Wilczek and M. Skowronek and K. Wiśniewska and F. Kaszuba and K. Surmiak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057501676&doi=10.1016%2fj.scitotenv.2018.11.374&partnerID=40&md5=920625e28f19c9227a0da1dee9ae8325},

doi = {10.1016/j.scitotenv.2018.11.374},

issn = {00489697},

year  = {2019},

date = {2019-01-01},

journal = {Science of the Total Environment},

volume = {656},

pages = {297-306},

publisher = {Elsevier B.V.},

abstract = {The study aimed at comparing the effects of short- and long-term exposure of Steatoda grossa female spiders to cadmium on the web's architecture, its energy content, and ultrastructure of ampullate glands. Simple food chain model (medium with 0.25 mM CdCl 2 → Drosophila hydei flies → spider (for 4 weeks or 12 months) was used for the exposure. Analysis of Cd content provided evidence that silk fibers of the web are well protected against its incorporation irrespectively of the exposure period. Long-term exposure to cadmium resulted in the occurrence of numerous autophagosomes with degenerated organelles as well as apoptotic and necrotic cells in the ampullate glands. Concurrently; the individual silk fibers building double and multiple combination complexes were significantly thinner than in the control threads. Moreover; exposed spiders spun net with smaller mean calorific value than did the control individuals. Hence; evaluation of both the diameter of silk fibers and calorific value of the web can serve as biomarkers of the effects caused by exposure of these predators to cadmium. © 2018 Elsevier B.V.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85049328870,

title = {Chronic toxicity of nanodiamonds can disturb development and reproduction of Acheta domesticus L},

author = { J. Karpeta-Kaczmarek and A. Kędziorski and M.A. Augustyniak-Jabłokow and M. Dziewięcka and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049328870&doi=10.1016%2fj.envres.2018.05.027&partnerID=40&md5=b4a7a9e96fecc2fbc5e750ec4afab8f1},

doi = {10.1016/j.envres.2018.05.027},

issn = {00139351},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Research},

volume = {166},

pages = {602-609},

publisher = {Academic Press Inc.},

abstract = {The use of nanodiamonds in numerous materials designed for industry and medicine is growing rapidly. Consequently health and environmental risks associated with the exposure of humans and other biota to nanodiamonds-based materials are of the utmost importance. Scarcity of toxicological data for these particles led us to examine the potentially deleterious effects of nanodiamonds in model insect species, Acheta domesticus (Orthoptera) chronically exposed to ND in its diet. Organism-level end-point indices (lifespan; body weight; consumption; caloric value of faeces; reproduction) revealed adverse changes in the treated crickets in comparison with the control. Preliminary studies of oxidative stress level in the offspring of ND-treated crickets suggest toxicity of these particles limited to the exposed individuals. EPR analysis showing increase of radical signal in the faeces of ND-fed crickets led us to propose novel mechanism of nanodiamonds toxicity that is discussed in the light of literature data. Capsule: Development and reproduction of Acheta domesticus can be disturbed by the chronic exposure to nanodiamonds © 2018 Elsevier Inc.},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85017565381,

title = {Lifespan differences between queens and workers are not explained by rates of molecular damage},

author = { E.R. Lucas and M. Augustyniak and A. Kędziorski and L. Keller},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017565381&doi=10.1016%2fj.exger.2017.03.008&partnerID=40&md5=7ca8f6159e66be170a28d1ca2bf202c0},

doi = {10.1016/j.exger.2017.03.008},

issn = {05315565},

year  = {2017},

date = {2017-01-01},

journal = {Experimental Gerontology},

volume = {92},

pages = {1-6},

publisher = {Elsevier Inc.},

abstract = {The biological processes that underlie senescence are of universal biological importance, yet they remain poorly understood. A popular theory proposes that senescence is the result of limited investment into mechanisms involved in the prevention and repair of molecular damage, leading to an accumulation of molecular damage with age. In ants, queen and worker lifespans differ by an order of magnitude, and this remarkable difference in lifespan has been shown to be associated with differences in the expression of genes involved in DNA and protein repair. Here we use the comet assay and Western Blotting for poly-ubiquitinated proteins to explore whether these differences in expression lead to differences in the accumulation of DNA damage (comet assay) or protein damage (protein ubiquitination) with age. Surprisingly, there was no difference between queens and workers in the rate of accumulation of DNA damage. We also found that levels of ubiquitinated proteins decreased with age, as previously reported in honeybees. This is in contrast to what has been found in model organisms such as worms and flies. Overall, these results reveal that the link between investment into macromolecular repair, age-related damage accumulation and lifespan is more complex than usually recognised. © 2017 Elsevier Inc.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85013411146,

title = {The effect of ingested cadmium on the calorific value and structural properties of hunting webs produced by Steatoda grossa (Theridiidae) spiders},

author = { G. Wilczek and J. Karcz and A. Putko and A. Kędziorski and P. Wilczek and M. Stalmach and E. Szulińska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013411146&doi=10.1016%2fj.scitotenv.2017.02.143&partnerID=40&md5=66965df01015a23dde39659aca4caffc},

doi = {10.1016/j.scitotenv.2017.02.143},

issn = {00489697},

year  = {2017},

date = {2017-01-01},

journal = {Science of the Total Environment},

volume = {586},

pages = {1298-1307},

publisher = {Elsevier B.V.},

abstract = {The study aimed to assess whether cadmium administered via ingestion to Steatoda grossa cobweb spiders (Theridiidae) affects the energy content and selected structural properties of the produced hunting webs. Cadmium content in webs was assessed with AAS and SEM X-ray microanalysis, while the diameters of silk fibers were estimated with SEM. The energy content of samples was measured in an oxygen micro-bomb calorimeter. Females and males showed different reactions to cadmium supplied through food. In comparison to females, males displayed higher metal concentrations in their bodies and hunting webs, however their calorific values and structural features were not significantly changed. Cadmium-treated females spun webs with smaller single-strand diameters and more frequent multi-stranded threads and invested 47% less energy in web production than the control individuals. It cannot be excluded that such a reduction in energy expenditure for web building in females resulted from energetically costly detoxifying reactions triggered in response to direct and indirect effects of cadmium toxicity. © 2017 Elsevier B.V.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84923569075,

title = {DNA damage in grasshopper Chorthippus brunneus (Orthoptera) hatchlings following paraquat exposure},

author = { M. Augustyniak and T. Nocon and A. Kędziorski and P. Łaszczyca and T. Sawczyn and M. Tarnawska and A. Zawisza-Raszka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923569075&doi=10.1016%2fj.chemosphere.2014.12.069&partnerID=40&md5=f1d2caec2debb3c806d9441a50130558},

doi = {10.1016/j.chemosphere.2014.12.069},

issn = {00456535},

year  = {2015},

date = {2015-01-01},

journal = {Chemosphere},

volume = {125},

pages = {212-219},

publisher = {Elsevier Ltd},

abstract = {Comet assay was applied to study genotoxic damage induced by paraquat (PQ) in brain cells of Chorthippus brunneus (Insecta: Orthoptera) hatchlings. Percentage of the comet fluorescence in the tail (TDNA), length of the comet tail (TL) and Olive tail moment (OTM) were used for quantitative assessment of the DNA damage. Multiple regression analysis supplemented standard statistical elaboration of the results. Increasing PQ concentrations applied either directly to the brain cells suspension (10; 50; and 250. μM PQ final concentration - in vitro protocol) or indirectly (50; 250; and 1250. μM PQ final concentration - in vivo protocol) provoked significant increase of oxidative damage to DNA (higher median TDNA and OTM values). The damage increased with time of exposure (0; 5; 15; and 30. min) following in vitro application, but decreased in longer interval (3 vs 24. h) after in vivo administration of paraquat. On contrary, median TL values did not correlate with paraquat concentration irrespectively of the exposure protocol. Possible reason of this discrepancy in light of paraquat toxicity is discussed. © 2015 Elsevier Ltd.},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84901241774,

title = {Parnassius apollo last-instar larvae development prediction by analysis of weather condition as a tool in the species' conservation},

author = { B. Łozowski and A. Kędziorski and M. Nakonieczny and P. Łaszczyca},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901241774&doi=10.1016%2fj.crvi.2014.03.004&partnerID=40&md5=c15d9fa248f59d5b88e594a7a6d4ae43},

doi = {10.1016/j.crvi.2014.03.004},

issn = {16310691},

year  = {2014},

date = {2014-01-01},

journal = {Comptes Rendus - Biologies},

volume = {337},

number = {5},

pages = {325-331},

publisher = {Elsevier Masson SAS},

abstract = {Parnassius apollo (Lepidoptera: Papilionidae) has already disappeared or is under threat of extinction in many of its former habitats. It has been documented that weather conditions - anomalies in particular - contributed to this process. In this study, we combined developmental data obtained previously for the last-instar Apollo larvae (collected in 1996; 1997; and 2003) with corresponding meteorological data to assess the effects of ambient temperature and rainfall episodes on the duration and the completion of the instar. For comparing the temperature effect, we applied the degree-day concept. We found significant positive correlation between the number of rainy days during the instar development (x) and its duration time (y): y = 8.293 + 0.936x (± 2.813) (r = 0.662; P < 10-7). Logarithmic transformation of the growth curves of the last-instar Apollo larvae revealed that there was no difference in growth among females; however, there was slower growth of males in 2003 in comparison to 1996. Growth (y) of female Apollo larvae as a function of instar duration (x) can by described by one common equation, irrespectively of the year: y = 317.6 + 502.3 lnx (± 263.3) (r = 0.82; P < 10 -4). © 2014 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84887612857,

title = {DNA damage in grasshoppers' larvae - Comet assay in environmental approach},

author = { M. Augustyniak and H. Orzechowska and A. Kędziorski and T. Sawczyn and B. Doleżych},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887612857&doi=10.1016%2fj.chemosphere.2013.10.033&partnerID=40&md5=5356747c3c954db75935f8623c8929d4},

doi = {10.1016/j.chemosphere.2013.10.033},

issn = {00456535},

year  = {2014},

date = {2014-01-01},

journal = {Chemosphere},

volume = {96},

pages = {180-187},

publisher = {Elsevier Ltd},

abstract = {The comet assay that provides a quantitative measure of the DNA-strand breaks may be used for assessing the 'genotoxic potential' of the environment. Young adults of Chorthippus brunneus (Orthoptera), collected at three sites in Southern Poland, differing in the level of pollution, particularly with heavy metals: Pilica (reference), Olkusz (moderately polluted) and Szopienice (heavily polluted) - were allowed to mate under laboratory conditions that were free from any pollution. Egg-pods were collected and, after diapause, brain cells from one-day old larvae were used for the comet assay. We compared the level of DNA damage in the larvae originating from these sites and also measured time-dependent DNA repair after single 10min. application of H2O2 (20μM final concentration).The DNA damage was relatively low in larval cells irrespectively of the site pollution their parents came from. However, measured comet parameters - tail DNA content (TDNA), tail length (TL), and olive tail moment (OTM) - were significantly higher in larvae originating from the Szopienice site than in those from the reference site. Incubation of cells with H2O2 resulted in significantly higher values of the comet parameters in the insects from all the study sites with the highest ones observed in the offspring of grasshoppers from Szopienice. Moreover, DNA repair, following the treatment, did not occur in the latter group. These data contribute to almost unexplored subject of genotoxic effects of environmental pollutants in insects. They are discussed in the light of the concept of adaptive strategies in energy allocation depending on the level of biotope pollution. © 2013 Elsevier Ltd.},

note = {29},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84864504690,

title = {The reproductive potential of the spiders Agelena labyrinthica and Xerolycosa nemoralis from areas contaminated with metals},

author = { A. Babczyńska and G. Wilczek and E. Szulińska and A. Kędziorski and I. Franiel and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864504690&doi=10.1016%2fj.scitotenv.2012.06.102&partnerID=40&md5=79b4edc840dd10b8ed2c4b4feb3386e5},

doi = {10.1016/j.scitotenv.2012.06.102},

issn = {00489697},

year  = {2012},

date = {2012-01-01},

journal = {Science of the Total Environment},

volume = {435-436},

pages = {374-379},

abstract = {Spiders successfully colonize industrially contaminated environments and maintain relatively stable populations. The aim of this study was to explain the reproductive strategies of two spider species, Xerolycosa nemoralis (an actively hunting; sit-and-pursue predator) and Agelena labyrinthica (a web. -building; sit-and-wait predator), between contaminated and uncontaminated sites. Spiders were collected from a reference site (Pilica) and two contaminated sites (Olkusz and Welnowiec). The amount of energy allocated to the eggs and the number of eggs and hatchlings as well as the hatching success were compared. Wolf spiders from the contaminated sites produced fewer but relatively energy-rich eggs, whereas web-building spiders invested their energy in the production of a higher number of less energy-rich eggs. The comparisons of the hatching percentages suggested that in the contaminated habitats, X. nemoralis achieve a hatching success similar to or higher than that of the reference population at Pilica. A. labyrinthica in the contaminated sites invested a larger amount of energy in eggs than at the reference site, but the hatching success found for this species in the contaminated areas was lower than that found at the reference site. © 2012 Elsevier B.V.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84861348718,

title = {Occurrence of Sarmentosin and Other Hydroxynitrile Glucosides in Parnassius (Papilionidae) Butterflies and Their Food Plants},

author = { N. Bjarnholt and M. Nakonieczny and A. Kędziorski and D.M. Debinski and S.F. Matter and C.E. Olsen and M. Zagrobelny},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84861348718&doi=10.1007%2fs10886-012-0114-x&partnerID=40&md5=a4d52b35eb6469e65a194910b73afb9e},

doi = {10.1007/s10886-012-0114-x},

issn = {00980331},

year  = {2012},

date = {2012-01-01},

journal = {Journal of Chemical Ecology},

volume = {38},

number = {5},

pages = {525-537},

abstract = {Sequestration of plant secondary metabolites is a widespread phenomenon among aposematic insects. Sarmentosin is an unsaturated γ-hydroxynitrile glucoside known from plants and some Lepidoptera. It is structurally and biosynthetically closely related to cyanogenic glucosides, which are commonly sequestered from food plants and/or de novo synthesized by lepidopteran species. Sarmentosin was found previously in Parnassius (Papilionidae) butterflies, but it was not known how the occurrence was related to food plants or whether Parnassius species could biosynthesize the compound. Here, we report on the occurrence of sarmentosin and related compounds in four different Parnassius species belonging to two different clades, as well as their known and suspected food plants. There were dramatic differences between the two clades, with P. apollo and P. smintheus from the Apollo group containing high amounts of sarmentosin, and P. clodius and P. mnemosyne from the Mnemosyne group containing low or no detectable amounts. This was reflected in the larval food plants; P. apollo and P. smintheus larvae feed on Sedum species (Crassulaceae), which all contained considerable amounts of sarmentosin, while the known food plants of the two other species, Dicentra and Corydalis (Fumariaceae), had no detectable levels of sarmentosin. All insects and plants containing sarmentosin also contained other biosynthetically related hydroxynitrile glucosides in patterns previously reported for plants, but not for insects. Not all findings could be explained by sequestration alone and we therefore hypothesize that Parnassius species are able to de novo synthesize sarmentosin. © 2012 Springer Science+Business Media, LLC.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33846888552,

title = {Midgut protease activities in monophagous larvae of Apollo butterfly, Parnassius apollo ssp. frankenbergeri},

author = { M. Nakonieczny and K. Michalczyk and A. Kędziorski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846888552&doi=10.1016%2fj.crvi.2006.12.002&partnerID=40&md5=07c8b860616c988e592952f39cfcd884},

doi = {10.1016/j.crvi.2006.12.002},

issn = {16310691},

year  = {2007},

date = {2007-01-01},

journal = {Comptes Rendus - Biologies},

volume = {330},

number = {2},

pages = {126-134},

abstract = {We assayed the relative activities of midgut proteolytic enzymes in individuals of the fourth (L4) and fifth (L5) instar of Apollo larvae, inhabiting Pieniny Mts (southern Poland). The comparisons between midgut tissue with glicocalyx (MT) and liquid midgut contents with peritrophic membrane (MC) were made. Optimal media pHs of the assayed proteolytic enzymes in P. apollo midgut samples were similar to those of other lepidopteran species. Endopeptidases, as well as carboxypeptidases, digested effectively in alkaline environment, while aminopeptidases were active in a broad pH range. Trypsin is probably the main endoprotease (correlation with caseinolytic activity in MC of L5 larvae: r = 0.606; p = 0.004); however, its activity was low as compared with that in other leaf-eating Lepidoptera. This suggests a minor role of trypsin and chymotrypsin in protein digestion in Apollo larvae, probably due to limited availability of the leaf proteins. Instead, due to very high carboxypeptidase A activity in midgut tissue, the larvae obtain exogenous amino acids either directly or from oligopeptides and glycoproteins. High and significant positive correlations between the enzyme activity and glucosidase as well as galactosidase activities strongly support this opinion. To cite this article: M. Nakonieczny et al., C. R. Biologies 330 (2007). © 2006 Académie des sciences.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33749254989,

title = {Midgut glycosidases activities in monophagous larvae of Apollo butterfly, Parnassius apollo ssp. frankenbergeri},

author = { M. Nakonieczny and K. Michalczyk and A. Kędziorski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749254989&doi=10.1016%2fj.crvi.2006.06.009&partnerID=40&md5=693bd6decf31c3de80afc9be2f2a0f49},

doi = {10.1016/j.crvi.2006.06.009},

issn = {16310691},

year  = {2006},

date = {2006-01-01},

journal = {Comptes Rendus - Biologies},

volume = {329},

number = {10},

pages = {765-774},

abstract = {Parnassius apollo (Lepidoptera; Papilionidae) declines on numerous localities all over Europe. Its local subspecies frankenbergeri, inhabiting the Pieniny Mts (southern Poland) and successfully recovered from extinction, is monophagous in larval stage. In natural conditions, it completes development on the orpine Sedum telephium ssp. maximum. Since proper quality and quantity of necessary nutritional compounds of the food plant ensure developmental success, the digestive processes in the insect midgut should reflect adaptation to a specific food source. The paper presents, for the first time, the activity of detected glycolytic enzymes in midgut tissue and liquid gut contents of the L4 and L5 instars of P. apollo larvae. α-Amylase plays the main role in utilization of carbohydrates, contrary to cellulase activity. Saccharase seems to be the main disaccharidase, and high activity of β-glycosidase enables hydrolysis of the plant glycosides. Trehalase activity was unexpectedly low and comparable to those of cellobiase and lactase. α-Amylolytic and other glycolytic activities indicate that larvae utilize starch and other carbohydrate compounds as energy sources. Possible use of some plant allelochemicals as energy sources by Apollo larvae is discussed. To cite this article: M. Nakonieczny et al., C. R. Biologies 329 (2006). © 2006 Académie des sciences.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-14844358518,

title = {Feeding preferences of the Apollo butterfly (Parnassius apollo ssp. frankenbergeri) larvae inhabiting the Pieniny Mts (southern Poland)},

author = { M. Nakonieczny and A. Kędziorski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-14844358518&doi=10.1016%2fj.crvi.2004.12.004&partnerID=40&md5=49685c75626b12af9e163010527fb4c7},

doi = {10.1016/j.crvi.2004.12.004},

issn = {16310691},

year  = {2005},

date = {2005-01-01},

journal = {Comptes Rendus - Biologies},

volume = {328},

number = {3},

pages = {235-242},

abstract = {Parnassius apollo (Lepidoptera; Papilionidae) is considered to be typical stenophagous species. Its European forms fall generally into 'telephiophagous' or 'albophagous' trophic groups. According to some authors, 'telephiophagous' P. apollo ssp. frankenbergeri Slaby, inhabiting the Pieniny Mts, has a rather broad spectrum of food-plants. We aimed at defining its feeding preferences for successful breeding in a semi-natural colony on more than one commercially-available Sedum species. Larval development (L5) and performance were assessed in three experimental groups fed on different plant species selected in a preliminary test. Apollo larvae appeared to be quite specific in the plant choice and developed poorly in the absence of Sedum telephium. Possible reasons of this are discussed. © 2004 Académie des sciences. Published by Elsevier SAS. All rights reserved.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0030369820,

title = {Cadmium-selenium antagonism and detoxifying enzymes in insects},

author = { A. Kędziorski and M. Nakonieczny and E. Świerczek and E. Szulińska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030369820&partnerID=40&md5=18eda2046c9653fdc406c19866c86013},

issn = {09370633},

year  = {1996},

date = {1996-01-01},

journal = {Fresenius' Journal of Analytical Chemistry},

volume = {354},

number = {5-6},

pages = {571-575},

abstract = {Activities of non-specific carboxylesterases (CarE) have been studied in adult males of hissing cockroach Gromphadorhina portentosa subjected to prolonged intoxication (7 weeks) with Cd and/or Se to investigate whether sublethal dose of cadmium (0.4 μmol, g-1 dry weight feed) would influence the detoxifying capabilities of the omnivorous insect, and whether selenium (0.01 μmol, g-1 dry weight feed) given concomitantly would act as Cd antagonist. Activities of CarE have been measured in crude homogenate, postmitochondrial supernatant and the pellet obtained from midgut, Malpighian tubules and fat body, using α-naphthyl acetate (αNA) and ρ-nitrophenyl acetate (ρNPA) as substrates. The highest rate of αNA hydrolysis occurred in midgut, whereas that of ρNPA in both the gut and Malpighian tubules. CarE activities in the Cd-group have been the highest in all tissues examined and for both substrates. A simultaneous administration of Cd and Se decreased the effects of Cd alone, probably as a result of partial elimination of the biologically active Cd pool. Selenium alone caused only minor effects when compared with cadmium. These results suggest, that disturbances of CarE activities depend on the Cd concentration in feed, and hence in tissues, as well as on possible interactions of the elements. © Springer-Verlag 1996.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}