@article{2-s2.0-85164024170,

title = {The Impact of Gold Nanoparticles on Somatic Embryogenesis Using the Example of Arabidopsis thaliana},

author = { K. Godel-Jędrychowska and A. Milewska-Hendel and K. Sala and R. Baranski and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85164024170&doi=10.3390%2fijms241210356&partnerID=40&md5=e32d3618b3f386a03753326e24a779bc},

doi = {10.3390/ijms241210356},

issn = {16616596},

year  = {2023},

date = {2023-01-01},

journal = {International Journal of Molecular Sciences},

volume = {24},

number = {12},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {Although the influence of nanoparticles (NPs) on developmental processes is better understood, little is known about their impact on somatic embryogenesis (SE). This process involves changes in the direction of cell differentiation. Thus, studying the effect of NPs on SE is essential to reveal their impact on cell fate. This study aimed to examine the influence of gold nanoparticles (Au NPs) with different surface charges on the SE of 35S:BBM Arabidopsis thaliana, with particular emphasis on the spatiotemporal localization of pectic arabinogalactan proteins (AGPs) and extensin epitopes in cells changing the direction of their differentiation. The results show that under the influence of nanoparticles, the explant cells of 35S:BBM Arabidopsis thaliana seedling origin did not enter the path of SE. Bulges and the formation of organ-like structures were observed in these explants, in contrast to the control, where somatic embryos developed. Additionally, spatiotemporal changes in the chemical composition of the cell walls during the culture were observed. Under the influence of Au NPs, the following effects were observed: (1) explant cells did not enter the SE pathway, (2) the impacts of Au NPs with different surface charges on the explants were variable, and (3) the compositions of the analyzed pectic AGPs and extensin epitopes were diverse in the cells with different developmental programs: SE (control) and non-SE (treated with Au NPs). © 2023 by the authors.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85160546790,

title = {Apoplastic and Symplasmic Markers of Somatic Embryogenesis},

author = { E.U. Kurczyńska and K. Godel-Jędrychowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160546790&doi=10.3390%2fplants12101951&partnerID=40&md5=a9124cfb5c9e46d3c91f66bb674ec020},

doi = {10.3390/plants12101951},

issn = {22237747},

year  = {2023},

date = {2023-01-01},

journal = {Plants},

volume = {12},

number = {10},

publisher = {MDPI},

abstract = {Somatic embryogenesis (SE) is a process that scientists have been trying to understand for many years because, on the one hand, it is a manifestation of the totipotency of plant cells, so it enables the study of the mechanisms regulating this process, and, on the other hand, it is an important method of plant propagation. Using SE in basic research and in practice is invaluable. This article describes the latest, but also historical, information on changes in the chemical composition of the cell wall during the transition of cells from the somatic to embryogenic state, and the importance of symplasmic communication during SE. Among wall chemical components, different pectic, AGP, extensin epitopes, and lipid transfer proteins have been discussed as potential apoplastic markers of explant cells during the acquisition of embryogenic competence. The role of symplasmic communication/isolation during SE has also been discussed, paying particular attention to the formation of symplasmic domains within and between cells that carry out different developmental processes. Information about the number and functionality of plasmodesmata (PD) and callose deposition as the main player in symplasmic isolation has also been presented. © 2023 by the authors.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85141878434,

title = {Rhodamine-Tagged Polymethacrylate Dyes as Alternative Tools for Analysis of Plant Cells},

author = { R. Bielas and J. Wróbel-Marek and E.U. Kurczyńska and D. Neugebauer},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85141878434&doi=10.3390%2fma15217720&partnerID=40&md5=4083debccc13d3ca7ff396c847ff55ac},

doi = {10.3390/ma15217720},

issn = {19961944},

year  = {2022},

date = {2022-01-01},

journal = {Materials},

volume = {15},

number = {21},

publisher = {MDPI},

abstract = {A rhodamine B (RhB)-based initiator for atom transfer radical polymerization (ATRP) was synthesized and applied for preparation of poly(2-trimethylammoniumethyl methacrylate) (PChMA), poly(2-hydroxyethyl methacrylate) (PHEMA) and poly(2-trimethylsilyloxyethyl methacrylate) (PHEMATMS). Polymer fluorescence was confirmed by determination of quantum yield by comparative method with piroxicam as the standard exhibiting dependency of emission intensity on the polymer chain hydrophilicity and the kind of solvent. The RhB functionalized polymers were used for biological tests in plant materials except for RhB-PHEMATMS because of weak fluorescence. These two polymers slightly differed in cellular localization. RhB-PChMA was mostly observed in cell walls of root tissues and cotyledon epidermis. It was also observed in cytoplasm and cell organelles of root cap cells and rhizodermis, in contrast with cytoplasm of cotyledon epidermis. RhB-PHEMA was also present in apoplast. A strong signal in protoxylem cell walls and a weak signal in cell walls of rhizodermis and cortex were visible. Moreover, it was also present in cell walls of cotyledon epidermis. However, RhB-PHEMA was mostly observed in cytoplasm and cell organelles of all root tissues and epidermis of cotyledons. Both RhB-polymers did not cause cell death which means that they can be used in living plant material. © 2022 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85127510342,

title = {Morphological, Histological and Ultrastructural Changes in Hordeum vulgare (L.) Roots That Have Been Exposed to Negatively Charged Gold Nanoparticles},

author = { A. Milewska-Hendel and W. Gepfert and M. Zubko and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85127510342&doi=10.3390%2fapp12073265&partnerID=40&md5=32e845d5c1fbcde8dd8ea646d8006723},

doi = {10.3390/app12073265},

issn = {20763417},

year  = {2022},

date = {2022-01-01},

journal = {Applied Sciences (Switzerland)},

volume = {12},

number = {7},

publisher = {MDPI},

abstract = {In recent years, there has been an impressive development of nanotechnology. This has resulted in the increasing release of nanomaterials (NM) into the environment, thereby causing the risk of an uncontrolled impact on living organisms, including plants. More studies indicated the biotoxic effect of NM on plants, including crops. The interaction of nanoparticles (NP) with food crops is extremely important as they are a link to the food chain. The objective of this study was to investigate the effect of negatively charged gold nanoparticles (-) AuNP (at two concentrations; 25 µg/mL or 50 µg/mL) on barley (Hordeum vulgare L.) root development. Morphological, histological and ultrastructural analyses (with the use of stereomicroscope; bright filed microscope and transmission electron microscope) revealed that regardless of the concentration, (-) AuNP did not enter into the plant body. However, the dose of (-) AuNP proved to be important for the plant’s response because different morphological, histological and ultrastructural changes were observed in the treated roots. The NP treatment caused: red root colouration, a local increase in the root diameter and a decreased formation of the root hair cells (on morphological level), damage to the rhizodermal cells, vacuolisation of the cortical cells, a detachment of the cell files between the cortical cells, atypical divisions of the cells, disorder of the meristem organisation (on the histological level), the appearance of periplasmic space, numerous vesicles and multivesicular bodies, electron-dense spots in cytoplasm, alterations in the structure of the mitochondria, breakdown of the tonoplast and the plasmalemma (on the ultrastructural level). © 2022 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85147518182,

title = {Doubled Haploids: Contributions of Poland's Academies in Recognizing the Mechanism of Gametophyte Cell Reprogramming and Their Utilization in Breeding of Agricultural and Vegetable Species},

author = { E.U. Kurczyńska and I. Żur and A. Adamus and T. Cegielska-Taras and S. Cichorz and E. Dubas and M. Gajecka and K. Juzoń-Sikora and A. Kiełkowska and A. Stra and S. Oleszczuk and E. Skrzypek and L. Szała and I. Szarejko and J. Zimny},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85147518182&doi=10.5586%2fASBP.9128&partnerID=40&md5=88ee571e2c6845abb015cfbe416e67ac},

doi = {10.5586/ASBP.9128},

issn = {00016977},

year  = {2022},

date = {2022-01-01},

journal = {Acta Societatis Botanicorum Poloniae},

volume = {91},

publisher = {Polish Botanical Society},

abstract = {Diverse processes leading to doubled haploid (DH) plant production, such as microspore embryogenesis, gynogenesis, and distant hybridization followed by genome elimination, are based on the unique ability of plant cells to form haploid embryos without fertilization. All of these are possible because of various in vitro culture systems that enable the growth and development of tissues or single cells outside of the parental organism. The possibility of re-directing cell development from its original pathway to embryogenesis brings several benefits to many research areas, but the most important is the possibility of its implementation in breeding programs. This review summarizes the achievements of Polish research groups in studies of the mechanisms of haploid/DH embryo development and demonstrates the practical applications of these systems in basic studies and plant breeding. It shows the results of studies on economically important crops including barley (Hordeum vulgare L.), oilseed rape (Brassica napus L.), triticale (×Triticosecale Wittm.), oat (Avena sativa L.), rye (Secale cereale L.), sugar beet (Beta vulgaris ssp. vulgaris L.), and some vegetable species, including carrot (Daucus carota L.), onion (Allium cepa L.), red beet (Beta vulgaris L.), and members of the Brassicaceae. © The Author(s) 2022.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85130021087,

title = {Symplasmic communication – supracellular system of regulation of cell differentiation [Komunikacja symplastowa – nadkomórkowy system regulacji różnicowania komórek]},

author = { K. Godel-Jędrychowska and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130021087&doi=10.18388%2fpb.2021_402&partnerID=40&md5=77a2c95d259e78cde3ec672c20f56a23},

doi = {10.18388/pb.2021_402},

issn = {00325422},

year  = {2022},

date = {2022-01-01},

journal = {Postepy Biochemii},

volume = {68},

number = {1},

pages = {3-14},

publisher = {Polish Biochemical Society},

abstract = {Plasmodesmata (PD), discovered more than 120 years ago, are still a mystery about their role in regulating plant cell differentiation. Research in recent years has verified our idea about the structure of PD and their function in the exchange of information between cells of the plant body. The involvement of PD in the movement of proteins, including transcription factors, hormones, and various types of RNA, indicates that they play an important role in regulating cell differentiation. The movement of molecules through PD is called symplasmic communication, and its limitations or absence are an essential element in controlling the direction of cell differentiation. © 2022, Polish Biochemical Society. All rights reserved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85120543114,

title = {Qualitative and quantitative analyses of the plasmodesmata that accompany cell fate changes during the somatic embryogenesis of Arabidopsis thaliana},

author = { K. Godel-Jędrychowska and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120543114&doi=10.1071%2fFP21243&partnerID=40&md5=279c14467f5420c126514ad4cbb3959d},

doi = {10.1071/FP21243},

issn = {14454408},

year  = {2022},

date = {2022-01-01},

journal = {Functional Plant Biology},

volume = {49},

number = {2},

pages = {186-200},

publisher = {CSIRO},

abstract = {Plasmodesmata (PD) are cytoplasmic and membrane-lined microchannels that enable symplasmic communication in plants, which is involved in the regulation of cell differentiation. The presented results emphasise the qualitative and quantitative analyses of PD, which are the basis of the symplasmic communication. The cells that initiate various development programmes create symplasmic domains that are characterised by different degrees of symplasmic communication. Changes in symplasmic communication are caused by the presence or absence of PD and/or the ability of signals to move through them. In the presented studies, somatic embryogenesis was used to describe the characteristics of the PD within and between the symplasmic domains in explants of the Arabidopsis thaliana (L.) Heynh ecotype Columbia-0 and 35S:BBM transgenic line. Transmission electron microscopy was used to describe the cells that regain totipotency/pluripotency during somatic embryogenesis, as well as the number and shape of the PD in the different symplasmic domains of the explants and somatic embryos. Array tomography was used to create a 3D reconstruction of the protodermal cells of the somatic embryos with particular emphasis on the PD distribution in the cell walls. The results showed that there were different frequencies of the PD within and between the symplasmic domain that emerges during somatic embryogenesis and between the Col-0 and 35S:BBM somatic embryos with regard to the differences in the shape of the PD. © 2022},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85115042302,

title = {Gold nanoparticles‐induced modifications in cell wall composition in barley roots},

author = { A. Milewska-Hendel and K. Sala and W. Gepfert and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115042302&doi=10.3390%2fcells10081965&partnerID=40&md5=22a9870ffeb8d79b61ecfc26ec5c8c5d},

doi = {10.3390/cells10081965},

issn = {20734409},

year  = {2021},

date = {2021-01-01},

journal = {Cells},

volume = {10},

number = {8},

publisher = {MDPI},

abstract = {The increased use of nanoparticles (NP) in different industries inevitably results in their release into the environment. In such conditions, plants come into direct contact with NP. Knowledge about the uptake of NP by plants and their effect on different developmental processes is still insufficient. Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP). The analyses were performed using the immunohistological method and fluorescence microscopy. The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85108635869,

title = {Nanoparticles—plant interaction: What we know, where we are?},

author = { E.U. Kurczyńska and K. Godel-Jędrychowska and K. Sala and A. Milewska-Hendel},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108635869&doi=10.3390%2fapp11125473&partnerID=40&md5=5739d81152baef30b3dc2ed0c4b3f0f7},

doi = {10.3390/app11125473},

issn = {20763417},

year  = {2021},

date = {2021-01-01},

journal = {Applied Sciences (Switzerland)},

volume = {11},

number = {12},

publisher = {MDPI AG},

abstract = {In recent years; the interaction of nanoparticles (NPs) with plants has been intensively studied. Therefore, more and more aspects related to both the positive and negative impact of NP on plants are well described. This article focuses on two aspects of NP interaction with plants. The first is a summary of the current knowledge on NP migration through the roots into the plant body, in particular, the role of the cell wall. The second aspect summarizes the current knowledge of the participation of the symplast, including the plasmodesmata (PD), in the movement of NP within the plant body. We highlight the gaps in our knowledge of the plant–NP interactions; paying attention to the need for future studies to explain the mechanisms that regulate the composition of the cell wall and the functioning of the PD under the influence of NP. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85108065626,

title = {Inhibition of carotenoid biosynthesis by crispr/cas9triggers cell wall remodelling in carrot},

author = { T. Oleszkiewicz and M. Klimek-Chodacka and M. Kruczek and K. Godel-Jędrychowska and K. Sala and A. Milewska-Hendel and M. Zubko and E.U. Kurczyńska and Y. Qi and R. Baranski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108065626&doi=10.3390%2fijms22126516&partnerID=40&md5=08bdce802b200c7f39ad88ac5c476848},

doi = {10.3390/ijms22126516},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {12},

publisher = {MDPI},

abstract = {Recent data indicate that modifications to carotenoid biosynthesis pathway in plants alter the expression of genes affecting chemical composition of the cell wall. Phytoene synthase (PSY) is a rate limiting factor of carotenoid biosynthesis and it may exhibit species-specific and or-gan-specific roles determined by the presence of psy paralogous genes, the importance of which often remains unrevealed. Thus, the aim of this work was to elaborate the roles of two psy paralogs in a model system and to reveal biochemical changes in the cell wall of psy knockout mutants. For this purpose, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (Cas9) proteins (CRISPR/Cas9) vectors were introduced to carotenoid-rich carrot (Daucus carota) callus cells in order to induce mutations in the psy1 and psy2 genes. Gene sequencing, expression analysis, and carotenoid content analysis revealed that the psy2 gene is critical for carotenoid bio-synthesis in this model and its knockout blocks carotenogenesis. The psy2 knockout also decreased the expression of the psy1 paralog. Immunohistochemical staining of the psy2 mutant cells showed altered composition of arabinogalactan proteins, pectins, and extensins in the mutant cell walls. In particular, low-methylesterified pectins were abundantly present in the cell walls of carote-noid-rich callus in contrast to the carotenoid-free psy2 mutant. Transmission electron microscopy revealed altered plastid transition to amyloplasts instead of chromoplasts. The results demonstrate for the first time that the inhibited biosynthesis of carotenoids triggers the cell wall remodelling. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85107550909,

title = {Similarities and Differences in the GFP Movement in the Zygotic and Somatic Embryos of Arabidopsis},

author = { K. Godel-Jędrychowska and K. Kulińska-Łukaszek and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107550909&doi=10.3389%2ffpls.2021.649806&partnerID=40&md5=4892959110fd3fda6312f875f34a1e9c},

doi = {10.3389/fpls.2021.649806},

issn = {1664462X},

year  = {2021},

date = {2021-01-01},

journal = {Frontiers in Plant Science},

volume = {12},

publisher = {Frontiers Media S.A.},

abstract = {Intercellular signaling during embryo patterning is not well understood and the role of symplasmic communication has been poorly considered. The correlation between the symplasmic domains and the development of the embryo organs/tissues during zygotic embryogenesis has only been described for a few examples, including Arabidopsis. How this process occurs during the development of somatic embryos (SEs) is still unknown. The aim of these studies was to answer the question: do SEs have a restriction in symplasmic transport depending on the developmental stage that is similar to their zygotic counterparts? The studies included an analysis of the GFP distribution pattern as expressed under diverse promoters in zygotic embryos (ZEs) and SEs. The results of the GFP distribution in the ZEs and SEs showed that 1/the symplasmic domains between the embryo organs and tissues in the SEs was similar to those in the ZEs and 2/the restriction in symplasmic transport in the SEs was correlated with the developmental stage and was similar to the one in their zygotic counterparts, however, with the spatio-temporal differences and different PDs SEL value between these two types of embryos. © Copyright © 2021 Godel-Jędrychowska, Kulińska-Łukaszek and Kurczyńska.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85097811034,

title = {Cell wall epitopes in grasses of different novel ecosystem habitats on post-industrial sites},

author = { A. Milewska-Hendel and D. Chmura and K. Wyrwał and E.U. Kurczyńska and A. Kompała-Bąba and A.M. Jagodziński and G. Woźniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097811034&doi=10.1002%2fldr.3786&partnerID=40&md5=b91f178a948f1d4383600818a1c0b390},

doi = {10.1002/ldr.3786},

issn = {10853278},

year  = {2021},

date = {2021-01-01},

journal = {Land Degradation and Development},

volume = {32},

number = {4},

pages = {1680-1694},

publisher = {John Wiley and Sons Ltd},

abstract = {The post-industrial habitats provide previously unknown conditions for plant and vegetation development. We asked the question: do the extreme soil substrate conditions cause differences in chemical composition of cell walls of leaves in three grass species: Calamagrostis epigejos (L.) Roth, Phragmites australis (Cav.) Trin. ex Steud, and Molinia caerulea (L.) Moench? With the use of immunohistochemical methods we determined the spatial distribution of selected pectic and AGP (arabinogalactan proteins) epitopes within leaf tissues of grass species growing in two industrial and control (meadow) habitats. Some post-industrial habitats increase biodiversity. Fast adaptation and divergence cause phenotypic changes. This process has been recently recorded and is called human-induced rapid evolutionary changes. The levels of pectins and arabinogalactan proteins increased on the post-industrial habitats in comparison to the control sites. On control habitat, pectic epitope with galactan chain residues (recognised by the LM5 antibody) was not detected in cell walls of Calamagrostis epigejos leaves, but was abundantly present in Phragmites australis leaves. The pectic epitope with arabinan residues (recognized by LM6 antibody) was less represented in cell walls of Molinia caerulea leaves. AGP epitope, recognised by LM2 antibody, was abundant only in Ph. australis. In Molinia caerulea this epitope was not detected. The AGP epitope recognised by JIM13 antibody was observed in all analysed species. The results obtained revealed the response of grass individuals to different environments: a) varied responses to different soil substratum conditions (novel ecosystems), and b) that this reaction is species-specific with respect to the analysed cell wall components. © 2020 John Wiley & Sons, Ltd.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85097655504,

title = {Germline development and seed set of metallophyte Biscutella laevigata L. (Brassicaceae): Reproductive biology of Biscutella laevigata L.},

author = { M. Kwiatkowska and K. Kłosowska and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097655504&doi=10.1016%2fj.flora.2020.151752&partnerID=40&md5=c225102035356992bae5635f4c0e1c55},

doi = {10.1016/j.flora.2020.151752},

issn = {03672530},

year  = {2021},

date = {2021-01-01},

journal = {Flora: Morphology, Distribution, Functional Ecology of Plants},

volume = {274},

publisher = {Elsevier GmbH},

abstract = {The costs of Biscutella laevigata adaptation, a facultative metallophyte, to an environment polluted with heavy metals were established by analyzing the differences in embryological processes between plants from two populations in Southern Poland (a mountain; in the Tatra Mountains and calamine; in Bolesław). Disturbances in male and female lineage development occurred in plants from both populations. The higher frequency of degenerations in plants from the calamine population could be interpreted as a strategy to save resources limited by the environment. The distribution of high-esterified homogalacturonan detected by LM20 antibody in the cell walls of embryos from the calamine population could be part of a resistance/defense system. The results from both populations indicate that B. laevigata has already developed adaptation/tolerance, enabling maintenance of the calamine population over time. Tolerant species could be an important source for revitalization and/or phytoremediation of polluted environments. © 2020 Elsevier GmbH},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85096384288,

title = {Symplasmic isolation marks cell fate changes during somatic embryogenesis},

author = { K. Godel-Jędrychowska and K. Kulińska-Łukaszek and A. Horstman and M. Soriano and M. Li and K. Małota and K. Boutilier and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096384288&doi=10.1093%2fJXB%2fERAA041&partnerID=40&md5=6d7f312b2384090f1b146b9ae888268e},

doi = {10.1093/JXB/ERAA041},

issn = {00220957},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Experimental Botany},

volume = {71},

number = {9},

pages = {2612-2628},

publisher = {Oxford University Press},

abstract = {Cell-to-cell signalling is a major mechanism controlling plant morphogenesis. Transport of signalling molecules through plasmodesmata is one way in which plants promote or restrict intercellular signalling over short distances. Plasmodesmata are membrane-lined pores between cells that regulate the intercellular flow of signalling molecules through changes in their size, creating symplasmic fields of connected cells. Here we examine the role of plasmodesmata and symplasmic communication in the establishment of plant cell totipotency, using somatic embryo induction from Arabidopsis explants as a model system. Cell-to-cell communication was evaluated using fluorescent tracers, supplemented with histological and ultrastructural analysis, and correlated with expression of a WOX2 embryo reporter. We showed that embryogenic cells are isolated symplasmically from non-embryogenic cells regardless of the explant type (immature zygotic embryos or seedlings) and inducer system (2;4-dichlorophenoxyacetic acid or the BABY BOOM (BBM) transcription factor), but that the symplasmic domains in different explants differ with respect to the maximum size of molecule capable of moving through the plasmodesmata. Callose deposition in plasmodesmata preceded WOX2 expression in future sites of somatic embryo development, but later was greatly reduced in WOX2expressing domains. Callose deposition was also associated with a decrease DR5 auxin response in embryogenic tissue. Treatment of explants with the callose biosynthesis inhibitor 2-deoxy-D-glucose supressed somatic embryo formation in all three systems studied, and also blocked the observed decrease in DR5 expression. Together these data suggest that callose deposition at plasmodesmata is required for symplasmic isolation and establishment of cell totipotency in Arabidopsis. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85088610736,

title = {Symplasmic isolation contributes to somatic embryo induction and development in the tree fern Cyathea delgadii Sternb},

author = { M. Grzyb and J. Wróbel-Marek and E.U. Kurczyńska and M. Sobczak and A. Mikuła},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088610736&doi=10.1093%2fPCP%2fPCAA058&partnerID=40&md5=105d33860cc4ed6eaa5fb78337e57815},

doi = {10.1093/PCP/PCAA058},

issn = {00320781},

year  = {2021},

date = {2021-01-01},

journal = {Plant and Cell Physiology},

volume = {61},

number = {7},

pages = {1273-1284},

publisher = {Oxford University Press},

abstract = {In this report, we describe studies on symplasmic communication and cellular rearrangement during direct somatic embryogenesis (SE) in the tree fern Cyathea delgadii. We analyzed changes in the symplasmic transport of low-molecular-weight fluorochromes, such as 8-hydroxypyr-ene-1,3,6-trisulfonic acid, trisodium salt (HPTS) and fluorescein (delivered to cells as fluorescein diacetate; FDA), within stipe explants and somatic embryos originating from single epidermal cells and developing during 16-d long culture. Induction of SE is preceded by a restriction in fluorochrome distribution between certain explant cells. Microscopic analysis showed a series of cellular changes like a decrease in vacuole size, increase in vacuole numbers, and increased density of cytoplasm and deposition of electron-dense material in cell walls that may be related with embryogenic transition. In somatic embryos, the limited symplasmic communication between cells was observed first in linear tri-cellular embryos. Further development of the fern embryo was associated with the formation of symplasmic domains corresponding to the four segments of the plant body. Using symplasmic tracers, we provided evidence that the changes in plasmodesmata permeability are corelated with somatic-to-embryogenic transition and somatic embryo development. © The Author(s) 2020. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85095603783,

title = {Cell wall composition as a marker of the reprogramming of the cell fate on the example of a daucus carota (L.) hypocotyl in which somatic embryogenesis was induced},

author = { M. Kuczak and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85095603783&doi=10.3390%2fijms21218126&partnerID=40&md5=9792b0cb8195f3030af0763fb8bc9f78},

doi = {10.3390/ijms21218126},

issn = {16616596},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Molecular Sciences},

volume = {21},

number = {21},

pages = {1-30},

publisher = {MDPI AG},

abstract = {Changes in the composition of the cell walls are postulated to accompany changes in the cell’s fate. We check whether there is a relationship between the presence of selected pectic, arabinogalactan proteins (AGPs), and extensins epitopes and changes in cell reprogramming in order to answer the question of whether they can be markers accompanying changes of cell fate. Selected antibodies were used for spatio-temporal immunolocalization of wall components during the induction of somatic embryogenesis. Based on the obtained results, it can be concluded that (1) the LM6 (pectic), LM2 (AGPs) epitopes are positive markers, but the LM5, LM19 (pectic), JIM8, JIM13 (AGPs) epitopes are negative markers of cells reprogramming to the meristematic/pluripotent state; (2) the LM8 (pectic), JIM8, JIM13, LM2 (AGPs) and JIM11 (extensin) epitopes are positive markers, but LM6 (pectic) epitope is negative marker of cells undergoing detachment; (3) JIM4 (AGPs) is a positive marker, but LM5 (pectic), JIM8, JIM13, LM2 (AGPs) are negative markers for pericycle cells on the xylem pole; (4) LM19, LM20 (pectic), JIM13, LM2 (AGPs) are constitutive wall components, but LM6, LM8 (pectic), JIM4, JIM8, JIM16 (AGPs), JIM11, JIM12 and JIM20 (extensins) are not constitutive wall components; (5) the extensins do not contribute to the cell reprogramming. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85091670721,

title = {Tocopherols mutual balance is a key player for maintaining Arabidopsis thaliana growth under salt stress},

author = { E. Surówka and I.W. Potocka and M.A. Dziurka and J. Wróbel-Marek and E.U. Kurczyńska and I. Żur and A. Maksymowicz and E. Gajewska and Z. Miszalski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091670721&doi=10.1016%2fj.plaphy.2020.09.008&partnerID=40&md5=178d47e55217a42bf706da8ce75b97d7},

doi = {10.1016/j.plaphy.2020.09.008},

issn = {09819428},

year  = {2020},

date = {2020-01-01},

journal = {Plant Physiology and Biochemistry},

volume = {156},

pages = {369-383},

publisher = {Elsevier Masson s.r.l.},

abstract = {Enhanced channeling carbon through pathways: shikimate/chorismate, benzenoid-phenylopropanoid or 2-C-methyl-D-erythritol 4-phosphate (MEP) provides a multitude of secondary metabolites and cell wall components and allows plants response to environmental stresses. Through the biosynthetic pathways, different secondary metabolites, like tocopherols (TCs), are bind to mutual dependencies and metabolic loops, that are not yet fully understood. We compared, in parallel, the influence of α- and γ-TCs on metabolites involved in osmoprotective/antioxidative response, and physico-chemical modification of plasma membrane and cell wall. We studied Arabidopsis thaliana Columbia ecotype (WT), mutant vte1 deficient in α- and γ-TCs, mutant vte4 over-accumulating γ-TC instead of α-TC, and transgenic line tmt over-accumulating α-TC; exposed to NaCl. The results indicate that salt stress activates β-carboxylation processes in WT plants and in plants with altered TCs accumulation. In α-TC-deficient plants, NaCl causes ACC decrease, but does not change SA, whose concentration remains higher than in α-TC accumulating plants. α/γ-TCs contents influence carbohydrates, poliamines, phenolic (caffeic; ferrulic; cinnamic) acids accumulation patterns. Salinity results in increased detection of the LM5 galactan and LM19 homogalacturonan epitopes in α-TC accumulating plants, and the LM6 arabinan and MAC207 AGP epitopes in α-TC deficient mutants. Parallel, plants with altered TCs composition show decreased both the cell turgor and elastic modulus determined at the individual cell level. α-TC deficient plants reveal lower values of cell turgor and elastic modulus, but higher cell hydraulic conductivity than α-TC accumulating plants. Under salt stress, α-TC shows stronger regulatory effect than γ-TC through the impact on chloroplastic biosynthetic pathways and ROS/osmotic-modulating compounds. © 2020 Elsevier Masson SAS},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85082955768,

title = {Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta},

author = { M. Popielarska-Konieczna and K. Sala and M. Abdullah and M. Tuleja and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85082955768&doi=10.1007%2fs00299-020-02530-2&partnerID=40&md5=731be893a36b20b90eaa9a5eabc144a5},

doi = {10.1007/s00299-020-02530-2},

issn = {07217714},

year  = {2020},

date = {2020-01-01},

journal = {Plant Cell Reports},

volume = {39},

number = {6},

pages = {779-798},

publisher = {Springer},

abstract = {Key message: Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit. Abstract: The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue. In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta. The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway. The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli. The organogenic tissue was compact and the outer walls of the peripheral cells were covered with granular structures. The non-organogenic tissue was composed of loosely attached cells, which were connected via a net-like structure. The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19; LM20; JIM11; JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus. Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25; heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5; LM6; LM19; JIM11; JIM12; JIM20; JIM8 and JIM16 epitopes). © 2020, The Author(s).},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85081036230,

title = {Development of embryo suspensors for five genera of crassulaceae with special emphasis on plasmodesmata distribution and ultrastructure},

author = { M. Kozieradzka-kiszkurno and D. Majcher and E. Brzezicka and J. Rojek and J. Wróbel-Marek and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081036230&doi=10.3390%2fplants9030320&partnerID=40&md5=4196b52894448b48c5bda10b06af6200},

doi = {10.3390/plants9030320},

issn = {22237747},

year  = {2020},

date = {2020-01-01},

journal = {Plants},

volume = {9},

number = {3},

publisher = {MDPI AG},

abstract = {The suspensor in the majority of angiosperms is an evolutionally conserved embryonic structure functioning as a conduit that connects ovule tissues with the embryo proper for nutrients and growth factors flux. This is the first study serving the purpose of investigating the correlation between suspensor types and plasmodesmata (PD), by the ultrastructure of this organ in respect of its full development. The special attention is paid to PD in representatives of Crassulaceae genera: Sedum, Aeonium, Monanthes, Aichryson and Echeveria. The contribution of the suspensor in transporting nutrients to the embryo was confirmed by the basal cell structure of the suspensor which produced, on the micropylar side of all genera investigated, a branched haustorium protruding into the surrounding ovular tissue and with wall ingrowths typically associated with cell transfer. The cytoplasm of the basal cell was rich in endoplasmic reticulum, mitochondria, dictyosomes, specialized plastids, microtubules, microbodies and lipid droplets. The basal cell sustained a symplasmic connection with endosperm and neighboring suspensor cells. Our results indicated the dependence of PD ultrastructure on the type of suspensor development: (i) simple PD are assigned to an uniseriate filamentous suspensor and (ii) PD with an electron-dense material are formed in a multiseriate suspensor. The occurrence of only one or both types of PD seems to be specific for the species but not for the genus. Indeed, in the two tested species of Sedum (with the distinct uniseriate/multiseriate suspensors), a diversity in the structure of PD depends on the developmental pattern of the suspensor. In all other genera (with the multiseriate type of development of the suspensor), the one type of electron-dense PD was observed. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85079083785,

title = {In vitro tissue culture in brachypodium: Applications and challenges},

author = { A. Betekhtin and K. Hus and M. Rojek-Jelonek and E.U. Kurczyńska and C. Nibau and J.H. Doonan and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079083785&doi=10.3390%2fijms21031037&partnerID=40&md5=f5b279c30079e29e77cf4ca674cd3454},

doi = {10.3390/ijms21031037},

issn = {16616596},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Molecular Sciences},

volume = {21},

number = {3},

publisher = {MDPI AG},

abstract = {Brachypodium distachyon has become an excellent model for plant breeding and bioenergy grasses that permits many fundamental questions in grass biology to be addressed. One of the constraints to performing research in many grasses has been the difficulty with which they can be genetically transformed and the generally low frequency of such transformations. In this review, we discuss the contribution that transformation techniques have made in Brachypodium biology as well as how Brachypodium could be used to determine the factors that might contribute to transformation efficiency. In particular, we highlight the latest research on the mechanisms that govern the gradual loss of embryogenic potential in a tissue culture and propose using B. distachyon as a model for other recalcitrant monocots. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85063231438,

title = {The development of a hairless phenotype in barley roots treated with gold nanoparticles is accompanied by changes in the symplasmic communication},

author = { A. Milewska-Hendel and W. Witek and A. Rypień and M. Zubko and R. Baranski and D. Stróż and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063231438&doi=10.1038%2fs41598-019-41164-7&partnerID=40&md5=458a420e10e45c07c747ba10f47a76f7},

doi = {10.1038/s41598-019-41164-7},

issn = {20452322},

year  = {2019},

date = {2019-01-01},

journal = {Scientific Reports},

volume = {9},

number = {1},

publisher = {Nature Publishing Group},

abstract = {Uptake of water and nutrients by roots affects the ontogenesis of the whole plant. Nanoparticles, e.g. gold nanoparticles, have a broad range of applications in many fields which leads to the transfer of these materials into the environment. Thus, the understanding of their impact on the growth and development of the root system is an emerging issue. During our studies on the effect of positively charged gold nanoparticles on the barley roots, a hairless phenotype was found. We investigated whether this phenotype correlates with changes in symplasmic communication, which is an important factor that regulates, among others, differentiation of the rhizodermis into hair and non-hair cells. The results showed no restriction in symplasmic communication in the treated roots, in contrast to the control roots, in which the trichoblasts and atrichoblasts were symplasmically isolated during their differentiation. Moreover, differences concerning the root morphology, histology, ultrastructure and the cell wall composition were detected between the control and the treated roots. These findings suggest that the harmful effect of nanoparticles on plant growth may, among others, consist in disrupting the symplasmic communication/isolation, which leads to the development of a hairless root phenotype, thus limiting the functioning of the roots. © 2019, The Author(s).},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85074563909,

title = {Composition of the reconstituted cell wall in protoplast-derived cells of daucus is affected by phytosulfokine (PSK)},

author = { K. Godel-Jędrychowska and K. Maćkowska and E.U. Kurczyńska and E. Grzebelus},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074563909&doi=10.3390%2fijms20215490&partnerID=40&md5=df2521002a27351a86ff912e9e654f86},

doi = {10.3390/ijms20215490},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {21},

publisher = {MDPI AG},

abstract = {Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation. It was shown that PSK stimulated and enhanced cell divisions in protoplast cultures of several species leading to callus and proembryogenic mass formation. Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process. So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells. Even less is known about the correlation between wall regeneration and the presence of PSK during the protoplast culture. Three Daucus taxa, including the cultivated carrot, were analyzed during protoplast regeneration. Several antibodies directed against wall components (anti-pectin: LM19; LM20; anti-AGP: JIM4; JIM8; JIM13 and anti-extensin: JIM12) were used. The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85068975939,

title = {Pyranine labeled polymer nanoparticles as fluorescent markers for cell wall staining and imaging of movement within apoplast},

author = { R. Bielas and J. Wróbel-Marek and E.U. Kurczyńska and D. Neugebauer},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068975939&doi=10.1016%2fj.snb.2019.126789&partnerID=40&md5=5d9eb379aecbe8c82237a8f741817dec},

doi = {10.1016/j.snb.2019.126789},

issn = {09254005},

year  = {2019},

date = {2019-01-01},

journal = {Sensors and Actuators, B: Chemical},

volume = {297},

publisher = {Elsevier B.V.},

abstract = {A new fluorescent initiator based on pyranine was synthesized and applied for synthesis of the water-soluble polymers by controlled radical polymerization. The fluorophore based polymethacrylates were dependent on chain content providing small nanoparticles in the range of 0.6–3.4 nm. Fluorescent properties corresponding to green light emission were verified by quantum yield and fluorescence intensity showing the influence of polymer type and solvent. 7-day-old seedlings of Arabidopsis thaliana Col-0 were used in analysis of the penetration/movement of fluorescent nanoparticles (FNPs) in roots and cotyledons. Obtained results showed that: i) the best fluorescent nanoparticles for biological studies appeared to be choline methacrylate containing polymer (HPTS-ChMA); ii) cell membrane is a barrier for FNPs and iii) HPTS-ChMA is efficient fluorescent marker of plant cell walls independently of plant organ. © 2019 Elsevier B.V.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85068749385,

title = {Aluminum alters the histology and pectin cell wall composition of barley roots},

author = { J. Jaskowiak and J. Kwaśniewska and A. Milewska-Hendel and E.U. Kurczyńska and M.E. Szurman-Zubrzycka and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068749385&doi=10.3390%2fijms20123039&partnerID=40&md5=4ea918ecebc6ab755c98c545ce00eb10},

doi = {10.3390/ijms20123039},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {12},

publisher = {MDPI AG},

abstract = {Aluminum (Al) is one of the most important crust elements causing reduced plant production in acidic soils. Barley (Hordeum vulgare L.) is considered to be one of the crops that is most sensitive to Al, and the root cell wall is the primary target of Al toxicity. In this study, we evaluate the possible involvement of specific pectic epitopes in the cells of barley roots in response to aluminum exposure. We targeted four different pectic epitopes recognized by LM5, LM6, LM19, and LM20 antibodies using an immunocytochemical approach. Since Al becomes available and toxic to plants in acidic soils, we performed our analyses on barley roots that had been grown in acidic conditions (pH 4.0) with and without Al and in control conditions (pH 6.0). Differences connected with the presence and distribution of the pectic epitopes between the control and Al-treated roots were observed. In the Al-treated roots, pectins with galactan sidechains were detected with a visually lower fluorescence intensity than in the control roots while pectins with arabinan sidechains were abundantly present. Furthermore, esterified homogalacturonans (HGs) were present with a visually higher fluorescence intensity compared to the control, while methyl-esterified HGs were present in a similar amount. Based on the presented results, it was concluded that methyl-esterified HG can be a marker for newly arising cell walls. Additionally, histological changes were detected in the roots grown under Al exposure. Among them, an increase in root diameter, shortening of root cap, and increase in the size of rhizodermal cells and divisions of exodermal and cortex cells were observed. The presented data extend upon the knowledge on the chemical composition of the cell wall of barley root cells under stress conditions. The response of cells to Al can be expressed by the specific distribution of pectins in the cell wall and, thus, enables the knowledge on Al toxicity to be extended by explaining the mechanism by which Al inhibits root elongation. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85061604241,

title = {Stability and instability processes in the calli of Fagopyrum tataricum that have different morphogenic potentials},

author = { A. Betekhtin and A. Piński and A. Milewska-Hendel and E.U. Kurczyńska and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061604241&doi=10.1007%2fs11240-019-01575-w&partnerID=40&md5=645591d8a95ce3e965e098b879213554},

doi = {10.1007/s11240-019-01575-w},

issn = {01676857},

year  = {2019},

date = {2019-01-01},

journal = {Plant Cell, Tissue and Organ Culture},

volume = {137},

number = {2},

pages = {343-357},

publisher = {Springer Netherlands},

abstract = {The morphogenic callus (MC) of Fagopyrum tataricum contains a large amount of flavonoids, especially rutin, and exhibits a high level of antioxidant activity. A non-morphogenic callus (NC) may appear on the surface of the MC after two to three years of cultivation and is then subjected to a consistently high level of oxidative stress. The elucidation of the molecular background of this instability is essential for gaining a better understanding of the somaclonal variation mechanisms in tissue cultures that have different morphogenic potentials. Thus, in this study we show that continuous oxidative stress in a NC might be connected with a rapid senescence process and as a result, in the upregulation of the genes that are connected with the telomere complexity, ethylene biosynthesis and the expression of DNA methyltransferases. Moreover, we analysed the presence of the hydroxyproline-rich glycoproteins in the calli and demonstrated the differences between the MC and NC. The LM2 antibody can be useful as a marker of the cells in the MC that are embryogenically determined, while the MAC207 antibody seems to be a positive marker of a MC as its signal was absent in the NC. This study also provides the first report on the effect of trichostatin A on the DNA methyltransferases and demethylases in a MC. © 2019, The Author(s).},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85066960172,

title = {Hydroxyproline-rich glycoproteins as markers of temperature stress in the leaves of brachypodium distachyon},

author = { A. Piński and A. Betekhtin and K. Sala and K. Godel-Jędrychowska and E.U. Kurczyńska and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066960172&doi=10.3390%2fijms20102571&partnerID=40&md5=83367841b3aa36bbcbe19816364135ad},

doi = {10.3390/ijms20102571},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {10},

publisher = {MDPI AG},

abstract = {Plants frequently encounter diverse abiotic stresses, one of which is environmental thermal stress. To cope with these stresses, plants have developed a range of mechanisms, including altering the cell wall architecture, which is facilitated by the arabinogalactan proteins (AGP) and extensins (EXT). In order to characterise the localisation of the epitopes of the AGP and EXT, which are induced by the stress connected with a low (4◦ C) or a high (40◦ C) temperature, in the leaves of Brachypodium distachyon, we performed immunohistochemical analyses using the antibodies that bind to selected AGP (JIM8; JIM13; JIM16; LM2 and MAC207), pectin/AGP (LM6) as well as EXT (JIM11; JIM12 and JIM20). The analyses of the epitopes of the AGP indicated their presence in the phloem and in the inner bundle sheath (JIM8; JIM13; JIM16 and LM2). The JIM16 epitope was less abundant in the leaves from the low or high temperature compared to the control leaves. The LM2 epitope was more abundant in the leaves that had been subjected to the high temperatures. In the case of JIM13 and MAC207, no changes were observed at the different temperatures. The epitopes of the EXT were primarily observed in the mesophyll and xylem cells of the major vascular bundle (JIM11; JIM12 and JIM20) and no correlation was observed between the presence of the epitopes and the temperature stress. We also analysed changes in the level of transcript accumulation of some of the genes encoding EXT, EXT-like receptor kinases and AGP in the response to the temperature stress. In both cases, although we observed the upregulation of the genes encoding AGP in stressed plants, the changes were more pronounced at the high temperature. Similar changes were observed in the expression profiles of the EXT and EXT-like receptor kinase genes. Our findings may be relevant for genetic engineering of plants with increased resistance to the temperature stress. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85064462014,

title = {Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union},

author = { K. Sala and J. Karcz and A. Rypień and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064462014&doi=10.1186%2fs12870-019-1748-4&partnerID=40&md5=b62468f34d858a8e2068f5b565af88af},

doi = {10.1186/s12870-019-1748-4},

issn = {14712229},

year  = {2019},

date = {2019-01-01},

journal = {BMC Plant Biology},

volume = {19},

number = {1},

publisher = {BioMed Central Ltd.},

abstract = {Background: Grafting is a technique widely used in horticulture. The processes involved in grafting are diverse, and the technique is commonly employed in studies focusing on the mechanisms that regulate cell differentiation or response of plants to abiotic stress. Information on the changes in the composition of the cell wall that occur during the grafting process is scarce. Therefore, this study was carried out for analyzing the composition of the cell wall using Arabidopsis hypocotyls as an example. During the study, the formation of a layer that covers the surface of the graft union was observed. So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union. Results: During grafting, polyphenolic and lipid compounds were detected, along with extracellular deposition of carbohydrate/protein material. The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union. These bead-like structures appeared either "closed" or "open". Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration. Conclusions: To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting. The results showed that unmethyl-esterified homogalacturonan and extensins are together involved in the adhesion of scion and stock, as well as taking part in sealing the graft union. The extracellular material is of importance not only due to the potential pectin-extensin interaction but also due to its origin. The findings presented here implicate a need for studies with biochemical approach for a detailed analysis of the composition and structure of the extracellular material. © 2019 The Author(s).},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85064930296,

title = {Effect of nanoparticles surface charge on the Arabidopsis thaliana (L.) roots development and their movement into the root cells and protoplasts},

author = { A. Milewska-Hendel and M. Zubko and D. Stróż and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064930296&doi=10.3390%2fijms20071650&partnerID=40&md5=1bd3b99f9fc8b4827645a064403650d4},

doi = {10.3390/ijms20071650},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {7},

publisher = {MDPI AG},

abstract = {Increasing usage of gold nanoparticles (AuNPs) in different industrial areas inevitably leads to their release into the environment. Thus, living organisms, including plants, may be exposed to a direct contact with nanoparticles (NPs). Despite the growing amount of research on this topic, our knowledge about NPs uptake by plants and their influence on different developmental processes is still insufficient. The first physical barrier for NPs penetration to the plant body is a cell wall which protects cytoplasm from external factors and environmental stresses. The absence of a cell wall may facilitate the internalization of various particles including NPs. Our studies have shown that AuNPs, independently of their surface charge, did not cross the cell wall of Arabidopsis thaliana (L.) roots. However, the research carried out with using light and transmission electron microscope revealed that AuNPs with different surface charge caused diverse changes in the root’s histology and ultrastructure. Therefore, we verified whether this is only the wall which protects cells against particles penetration and for this purpose we used protoplasts culture. It has been shown that plasma membrane (PM) is not a barrier for positively charged (+) AuNPs and negatively charged (−) AuNPs, which passage to the cell. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85057991726,

title = {Cell wall epitopes and endoploidy as reporters of embryogenic potential in brachypodium distachyon callus culture},

author = { A. Betekhtin and M. Rojek-Jelonek and K. Nowak and A. Piński and A. Milewska-Hendel and E.U. Kurczyńska and J.H. Doonan and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057991726&doi=10.3390%2fijms19123811&partnerID=40&md5=a019f7df04ed76fd5a1440b62abca7ae},

doi = {10.3390/ijms19123811},

issn = {16616596},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Molecular Sciences},

volume = {19},

number = {12},

publisher = {MDPI AG},

abstract = {Effective regeneration of callus tissue into embryos and then into whole plants is essential for plant biotechnology. The embryonic potential is often low and can further decrease with time in culture, which limits the utilisation of calli for transformation procedures and in vitro propagation. In this study, we show that the loss of embryogenic potential in callus cultures of Brachypodium distachyon is progressive over time. Flow cytometry analyses indicated endoploidy levels increased in 60-and 90-day-old calli with effective loss of the 2C DNA content peak in the latter. Analysis of indolic compounds content revealed a decrease in 60-and 90-day-old calli compared to either freshly isolated explants or 30-day-old calli. Immunohistochemical analysis revealed a decrease in arabinogalactan proteins (AGP) signal with the time of culture, but extensin (EXT) epitopes either increased (JIM12 epitopes) or decreased (JIM11 epitopes). The transcript accumulation levels of AGPs and EXTs confirmed these results, with most of AGP and EXT transcripts gradually decreasing. Some chimeric EXT transcripts significantly increased on the 30th day of culture, perhaps because of an increased embryogenic potential. Selected somatic embryogenesis-related genes and cyclins demonstrated a gradual decrease of transcript accumulation for YUCCA (YUC), AINTEGUMENTA-LIKE (AIL), BABY BOOM (BBM), and CLAVATA (CLV3) genes, as well as for most of the cyclins, starting from the 30th day of culture. Notably, WUSCHEL (WUS) transcript was detectable only on the 30th and 60th day and was not detectable in the zygotic embryos and in 90-day-old calli. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85052124909,

title = {Unique chromoplast organisation and carotenoid gene expression in carotenoid-rich carrot callus},

author = { T. Oleszkiewicz and M. Klimek-Chodacka and A. Milewska-Hendel and M. Zubko and D. Stróż and E.U. Kurczyńska and A. Boba and J. Szopa and R. Baranski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052124909&doi=10.1007%2fs00425-018-2988-5&partnerID=40&md5=b60366c39577f353d0cbea077a81b5ba},

doi = {10.1007/s00425-018-2988-5},

issn = {00320935},

year  = {2018},

date = {2018-01-01},

journal = {Planta},

volume = {248},

number = {6},

pages = {1455-1471},

publisher = {Springer Verlag},

abstract = {Main conclusion: The new model orange callus line, similar to carrot root, was rich in carotenoids due to altered expression of some carotenogenesis-associated genes and possessed unique diversity of chromoplast ultrastructure. Callus induced from carrot root segments cultured in vitro is usually pale yellow (p-y) and poor in carotenoids. A unique, non-engineered callus line of dark orange (d-o) colour was developed in this work. The content of carotenoid pigments in d-o callus was at the same level as in an orange carrot storage root and nine-fold higher than in p-y callus. Carotenoids accumulated mainly in abundant crystalline chromoplasts that are also common in carrot root but not in p-y callus. Using transmission electron microscopy, other types of chromoplasts were also found in d-o callus, including membranous chromoplasts rarely identified in plants and not observed in carrot root until now. At the transcriptional level, most carotenogenesis-associated genes were upregulated in d-o callus in comparison to p-y callus, but their expression was downregulated or unchanged when compared to root tissue. Two pathway steps were critical and could explain the massive carotenoid accumulation in this tissue. The geranylgeranyl diphosphate synthase gene involved in the biosynthesis of carotenoid precursors was highly expressed, while the β-carotene hydroxylase gene involved in β-carotene conversion to downstream xanthophylls was highly repressed. Additionally, paralogues of these genes and phytoene synthase were differentially expressed, indicating their tissue-specific roles in carotenoid biosynthesis and metabolism. The established system may serve as a novel model for elucidating plastid biogenesis that coincides with carotenogenesis. © 2018, The Author(s).},

note = {20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85048889878,

title = {5-Azacitidine induces cell death in a tissue culture of brachypodium distachyon},

author = { A. Betekhtin and A. Milewska-Hendel and Ł. Chajec and M. Rojek-Jelonek and K. Nowak and J. Kwaśniewska and E.A. Wolny and E.U. Kurczyńska and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048889878&doi=10.3390%2fijms19061806&partnerID=40&md5=72a6b817774ef0346f00f6530b07a6ac},

doi = {10.3390/ijms19061806},

issn = {16616596},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Molecular Sciences},

volume = {19},

number = {6},

publisher = {MDPI AG},

abstract = {Morphological and histological observations revealed that, at a concentration of 50 µM, 5-azacitidine (5-azaC) totally inhibited the induction of embryogenic masses (EM), while the cultivation of explants (zygotic embryos; ZEs) in the presence of 5 µM of 5-azaC led to the formation of a callus with EM in 10% of the cases. Transmission electron microscopy (TEM) analyzes revealed the presence of the morphological and ultrastructural features that are typical for the vacuolar type of cell death in the callus cells that were treated. A TUNEL assay confirmed the presence of DNA double-strand breaks for the callus cells that had been treated with both 5 and 50 µM 5-azaC concentrations. Analysis of the gene expression of selected cell death markers demonstrated a reduced expression of metacaspase, protein executer 1 (EX1), and thioredoxin (TRX) in the callus cells that had been treated compared to the control culture. The strongest increase in the gene activity was characteristic for glutathione S-transferase (GST). Our studies also included an analysis of the distribution of some arabinogalactan proteins (AGPs) and extensin epitopes, which can be used as markers of cells that are undergoing death in a Brachypodium distachyon tissue culture. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85046167557,

title = {Spatio-temporal localization of selected pectic and arabinogalactan protein epitopes and the ultrastructural characteristics of explant cells that accompany the changes in the cell fate during somatic embryogenesis in Arabidopsis thaliana},

author = { I.W. Potocka and K. Godel and I. Dobrowolska and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046167557&doi=10.1016%2fj.plaphy.2018.04.032&partnerID=40&md5=df32e859e10de06c7f7f8dfa7fbcbe6d},

doi = {10.1016/j.plaphy.2018.04.032},

issn = {09819428},

year  = {2018},

date = {2018-01-01},

journal = {Plant Physiology and Biochemistry},

volume = {127},

pages = {573-589},

publisher = {Elsevier Masson SAS},

abstract = {During somatic embryogenesis (SE), explant cells undergo changes in the direction of their differentiation, which lead to diverse cell phenotypes. Although the genetic bases of the SE have been extensively studied in Arabidopsis thaliana, little is known about the chemical characteristics of the wall of the explant cells, which undergo changes in the direction of differentiation. Thus, we examined the occurrence of selected pectic and AGP epitopes in explant cells that display different phenotypes during SE. Explants examinations have been supplemented with an analysis of the ultrastructure. The deposition of selected pectic and AGP epitopes in somatic embryos was determined. Compared to an explant at the initial stage, a/embryogenic/totipotent and meristematic/pluripotent cells were characterized by a decrease in the presence of AGP epitopes, b/the presence of AGP epitopes in differentiated cells was similar, and c/an increase of analyzed epitopes was detected in the callus cells. Totipotent cells could be distinguished from pluripotent cells by: 1/the presence of the LM2 epitope in the latest one, 2/the appearance of the JIM16 epitope in totipotent cells, and 3/the more abundant presence of the JIM7 epitope in the totipotent cells. The LM5 epitope characterized the wall of the cells that were localized within the mass of embryogenic domain. The JIM8, JIM13 and JIM16 AGP epitopes appeared to be the most specific for the callus cells. The results indicate a relationship between the developmental state of the explant cells and the chemical composition of the cell walls. © 2018 The Authors},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85042854293,

title = {Organ and tissue-specific localisation of selected cell wall epitopes in the zygotic embryo of Brachypodium distachyon},

author = { A. Betekhtin and A. Milewska-Hendel and J. Lusinska and Ł. Chajec and E.U. Kurczyńska and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042854293&doi=10.3390%2fijms19030725&partnerID=40&md5=f697b77194807b245593766f2c341404},

doi = {10.3390/ijms19030725},

issn = {16616596},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Molecular Sciences},

volume = {19},

number = {3},

publisher = {MDPI AG},

abstract = {The plant cell wall shows a great diversity regarding its chemical composition, which may vary significantly even during different developmental stages. In this study, we analysed the distribution of several cell wall epitopes in embryos of Brachypodium distachyon (Brachypodium). We also described the variations in the nucleus shape and the number of nucleoli that occurred in some embryo cells. The use of transmission electron microscopy, and histological and immunolocalisation techniques permitted the distribution of selected arabinogalactan proteins, extensins, pectins, and hemicelluloses on the embryo surface, internal cell compartments, and in the context of the cell wall ultrastructure to be demonstrated. We revealed that the majority of arabinogalactan proteins and extensins were distributed on the cell surface and that pectins were the main component of the seed coat and other parts, such as the mesocotyl cell walls and the radicula. Hemicelluloses were localised in the cell wall and outside of the radicula protodermis, respectively. The specific arrangement of those components may indicate their significance during embryo development and seed germination, thus suggesting the importance of their protective functions. Despite the differences in the cell wall composition, we found that some of the antibodies can be used as markers to identify specific cells and the parts of the developing Brachypodium embryo. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85020376429,

title = {Fate of neutral-charged gold nanoparticles in the roots of the Hordeum vulgare L. cultivar Karat},

author = { A. Milewska-Hendel and M. Zubko and J. Karcz and D. Stróż and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020376429&doi=10.1038%2fs41598-017-02965-w&partnerID=40&md5=491d1da7c9f8fdf8bb31eb6e751eafff},

doi = {10.1038/s41598-017-02965-w},

issn = {20452322},

year  = {2017},

date = {2017-01-01},

journal = {Scientific Reports},

volume = {7},

number = {1},

publisher = {Nature Publishing Group},

abstract = {Nanoparticles (NPs) have a significant impact on the environment and living organisms. The influence of NPs on plants is intensively studied and most of the data indicate that NPs can penetrate into plants. The studies presented here were performed on the roots of Hordeum vulgare L. seedlings using neutral-charge gold nanoparticles (AuNPs) of different sizes. In contrast to the majority of the published data, the results presented here showed that during the culture period, AuNPs: 1/did not enter the root regardless of their size and concentration, 2/that are applied directly into the cells of a root do not move into neighbouring cells. The results that were obtained indicate that in order to extend our knowledge about the mechanisms of the interactions between NPs and plants, further studies including, among others, on different species and a variety of growth conditions are needed. © 2017 The Author(s).},

note = {32},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85017512737,

title = {In Memoriam: Zygmunt Hejnowicz (1929-2016)},

author = { D. Kwiatkowska and J. Nakielski and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017512737&doi=10.1080%2f15592324.2017.1303593&partnerID=40&md5=e468d7d360154393bf0fbc1fd32dc84d},

doi = {10.1080/15592324.2017.1303593},

issn = {15592324},

year  = {2017},

date = {2017-01-01},

journal = {Plant signaling & behavior},

volume = {12},

number = {4},

pages = {e1303593-},

abstract = {Professor Zygmunt Hejnowicz passed away aged 87, on the first of May 2016. We describe his major research interests and contribution to plant development, anatomy, and biophysics, from the perspective of his close collaborators.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85014957702,

title = {Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn},

author = { A. Betekhtin and M. Rojek-Jelonek and J. Jaskowiak and A. Milewska-Hendel and J. Kwaśniewska and Y. Kostyukova and E.U. Kurczyńska and N. Rumyantseva and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85014957702&doi=10.1371%2fjournal.pone.0173537&partnerID=40&md5=8a4e7fc7b0fee91c193c03db23eaecb2},

doi = {10.1371/journal.pone.0173537},

issn = {19326203},

year  = {2017},

date = {2017-01-01},

journal = {PLoS ONE},

volume = {12},

number = {3},

publisher = {Public Library of Science},

abstract = {Long-term cultivated Fagopyrum tataricum (L.) Gaertn. (Tartary buckwheat) morphogenic and non-morphogenic callus lines are interesting systems for gaining a better understanding of the mechanisms that are responsible for the genetic stability and instability of a plant tissue culture. In this work, we used histological sections and transmission electron microscopy to identify and describe the morphology of the nuclei of all of the analysed callus lines. We demonstrated that the embryogenic callus cells had prominent round nuclei that did not contain heterochromatin clumps in contrast to the non-morphogenic callus lines, in which we found nuclei that had multiple lobes. Flow cytometry analysis revealed significant differences in the relative DNA content between the analysed calli. All of the analysed morphogenic callus lines had peaks from 2C to 8C as compared to the nonmorphogenic callus lines, whose peaks did not reflect any regular DNA content and exceeded 8C and 16C for the line 6p1 and 16C and 32C for the callus line 10p2A. The results showed that non-morphogenic calli are of an aneuploid nature. The TUNEL test enabled us to visualise the nuclei that had DNA fragmentation in both the morphogenic and non-morphogenic lines. We revealed significantly higher frequencies of positively labelled nuclei in the non-morphogenic lines than in the morphogenic lines. In the case of the morphogenic lines, the highest observed frequency of TUNEL-positive nuclei was 7.7% for lines 2-3. In the non-morphogenic calli, the highest level of DNA damage (68.5%) was revealed in line 6p1. These results clearly indicate greater genome stability in the morphogenic lines. © 2017 Betekhtin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84997241523,

title = {Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae)},

author = { J. Wróbel-Marek and E.U. Kurczyńska and B.J. Płachno and M. Kozieradzka-kiszkurno},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84997241523&doi=10.1007%2fs00425-016-2619-y&partnerID=40&md5=2a690116fd6fb77c5f461a1f69c931fd},

doi = {10.1007/s00425-016-2619-y},

issn = {00320935},

year  = {2017},

date = {2017-01-01},

journal = {Planta},

volume = {245},

number = {3},

pages = {491-505},

publisher = {Springer Verlag},

abstract = {Main conclusion: Our study demonstrated that symplasmic communication betweenSedum acreseed compartments and the embryo proper is not uniform. The presence of plasmodesmata (PD) constitutes the structural basis for information exchange between cells, and symplasmic communication is involved in the regulation of cell differentiation and plant development. Most recent studies concerning an analysis of symplasmic communication between seed compartments and the embryo have been predominantly performed on Arabidopsis thaliana. The results presented in this paper describe the analysis of symplasmic communication on the example of Sedum acre seeds, because the ultrastructure of the seed compartments and the embryo proper, including the PD, have already been described, and this species represents an embryonic type of development different to Arabidopsis. Moreover, in this species, an unusual electron-dense dome associated with plasmodesmata on the border between the basal cell/chalazal suspensor cells and the basal cell/the endosperm has been described. This prompted the question as to whether these plasmodesmata are functional. Thus, the aim of this study was to describe the movement of symplasmic transport fluorochromes between different Sedum seed compartments, with particular emphasis on the movement between the basal cell and the embryo proper and endosperm, to answer the following questions: (1) are seeds divided into symplasmic domains; (2) if so, are they stable or do they change with the development? The results have shown that symplasmic tracers movement: (a) from the external integument to internal integument is restricted; (b) from the basal cell to the other part of the embryo proper and from the basal cell to the endosperm is also restricted; (c) the embryo is a single symplasmic domain with respect to molecules of a molecular weight below 0.5 kDa. © 2016, The Author(s).},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84966421090,

title = {Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae)},

author = { R. Gawecki and K. Sala and E.U. Kurczyńska and P. Świątek and B.J. Płachno},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966421090&doi=10.1007%2fs00709-016-0980-0&partnerID=40&md5=5294556454d52366eb2f810219227c05},

doi = {10.1007/s00709-016-0980-0},

issn = {0033183X},

year  = {2017},

date = {2017-01-01},

journal = {Protoplasma},

volume = {254},

number = {2},

pages = {657-668},

publisher = {Springer-Verlag Wien},

abstract = {In apomictic Taraxacum species, the development of both the embryo and the endosperm does not require double fertilisation. However, a structural reduction of ovular transmitting tissue was not observed in apomictic dandelions. The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum. The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum. © 2016, The Author(s).},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85013892983,

title = {Quantitative and qualitative characteristics of cell wall components and prenyl lipids in the leaves of Tilia x euchlora trees growing under salt stress},

author = { A. Milewska-Hendel and A.H. Baczewska and K. Sala and W. Dmuchowski and P. Bragoszewska and D. Gozdowski and A. Jozwiak and T. Chojnacki and E. Swiezewska and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013892983&doi=10.1371%2fjournal.pone.0172682&partnerID=40&md5=9273a1f5b327a5d5316ca4927ba2751f},

doi = {10.1371/journal.pone.0172682},

issn = {19326203},

year  = {2017},

date = {2017-01-01},

journal = {PLoS ONE},

volume = {12},

number = {2},

publisher = {Public Library of Science},

abstract = {The study was focused on assessing the presence of arabinogalactan proteins (AGPs) and pectins within the cell walls as well as prenyl lipids, sodium and chlorine content in leaves of Tilia x euchlora trees. The leaves that were analyzed were collected from trees with and without signs of damage that were all growing in the same salt stress conditions. The reason for undertaking these investigations was the observations over many years that indicated that there are trees that present a healthy appearance and trees that have visible symptoms of decay in the same habitat. Leaf samples were collected from trees growing in the median strip between roadways that have been intensively salted during the winter season for many years. The sodium content was determined using atomic spectrophotometry, chloride using potentiometric titration and poly-isoprenoids using HPLC/UV. AGPs and pectins were determined using immunohistochemistry methods. The immunohistochemical analysis showed that rhamnogalacturonans I (RG-I) and homogalacturonans were differentially distributed in leaves from healthy trees in contrast to leaves from injured trees. In the case of AGPs, the most visible difference was the presence of the JIM16 epitope. Chemical analyses of sodium and chloride showed that in the leaves from injured trees, the level of these ions was higher than in the leaves from healthy trees. Based on chromatographic analysis, four polyisoprenoid alcohols were identified in the leaves of T. x euchlora. The levels of these lipids were higher in the leaves from healthy trees. The results suggest that the differences that were detected in the apoplast and symplasm may be part of the defensive strategy of T. x euchlora trees to salt stress, which rely on changes in the chemical composition of the cell wall with respect to the pectic and AGP epitopes and an increased synthesis of prenyl lipids. © 2017 Milewska-Hendel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85010756219,

title = {Distribution of some pectic and arabinogalactan protein epitopes during Solanum lycopersicum (L.) adventitious root development},

author = { K. Sala and K. Malarz and P.W. Barlow and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85010756219&doi=10.1186%2fs12870-016-0949-3&partnerID=40&md5=36843d57a835eaa1f1f38157bc92d206},

doi = {10.1186/s12870-016-0949-3},

issn = {14712229},

year  = {2017},

date = {2017-01-01},

journal = {BMC Plant Biology},

volume = {17},

number = {1},

publisher = {BioMed Central Ltd.},

abstract = {Background: The adventitious roots (AR) of plants share the same function as primary and lateral roots (LR), although their development is mainly an adaptive reaction to stress conditions. Regeneration of grafted plants is often accompanied by AR formation thus making the grafting technique a good model for studying AR initiation and development and their means of emergence. Pectins and arabinogalactan proteins (AGP) are helpful markers of particular cellular events, such as programmed cell death (PCD), elongation, proliferation or other differentiation events that accompany AR development. However, little is known about the distribution of pectins and AGPs during AR ontogeny, either in the primordium or stem tissues from which AR arise or their correspondence with these events during LR formation. Results: AR were developed from different stem tissues such as parenchyma, xylem rays and the cambium, depending on the stem age and treatment (grafting versus cutting) of the parental tissue. Immunochemical analysis of the presence of pectic (LM8; LM19; LM20) and AGP (JIM8; JIM13; JIM16) epitopes in AR and AR-associated tissues showed differential, tissue-specific distributions of these epitopes. Two pectic epitopes (LM19; LM20) were developmentally regulated and the occurrence of the LM8 xylogalacturonan epitope in the root cap of the AR differed from other species described so far. AGP epitopes were abundantly present in the cytoplasmic compartments (mainly the tonoplast) and were correlated with the degree of cell vacuolisation. JIM8 and JIM13 epitopes were detected in the more advanced stages of primordium development, whereas the JIM16 epitope was present from the earliest division events of the initial AR cells. The comparison between AR and LR showed quantitative (AGP;) and qualitative (pectins) differences. Conclusion: The chemical compositions of adventitious and lateral root cells show differences that correlate with the different origins of these cells. In AR, developmental changes in the distribution of pectins and AGP suggest the turnover of wall compounds. Our data extend the knowledge about the distribution of pectin and AGP during non-embryogenic root development in a species that is important from an agronomic point of view. © 2017 The Author(s).},

note = {21},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021764617,

title = {Morpho-histological analysis of tomato (Solanum lycopersicum L.) plants after treatment with juglone},

author = { A. Milewska-Hendel and M. Polak and K. Sala and P. Zieleźnik-Rusinowska and R. Gawecki and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021764617&doi=10.5586%2faa.1701&partnerID=40&md5=08218efe0b5c44652666a966d3d5eeee},

doi = {10.5586/aa.1701},

issn = {00650951},

year  = {2017},

date = {2017-01-01},

journal = {Acta Agrobotanica},

volume = {70},

number = {2},

publisher = {Polish Botanical Society},

abstract = {Juglone is a substance that limits plant growth and has a toxic effect on plant development. In this study, we analyzed the influence of juglone at two different concentrations (10-3 M and 10-4 M), which were applied to different parts of Solanum lycopersicum L. plants (root system; stem after decapitation; and surface of a younger leaf or after autografting) for a short period of time (7 days), on the morphology and histology of stems. At a lower concentration, juglone had positive effects on plant growth, which resulted in an increase in interfascicular cambial cell divisions, faster development of a continuous cambium layer along the stem circumference, and development of fibers. Additionally, under the influence of juglone, the number of developing leaves increased and adventitious roots developed. The results are discussed based on the current literature concerning the reaction of plants to juglone and to stress conditions. © The Author(s) 2017.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84997354235,

title = {Spatial distribution of selected chemical cell wall components in the embryogenic callus of brachypodium distachyon},

author = { A. Betekhtin and M. Rojek-Jelonek and A. Milewska-Hendel and R. Gawecki and J. Karcz and E.U. Kurczyńska and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84997354235&doi=10.1371%2fjournal.pone.0167426&partnerID=40&md5=d5dadb0308d02b7c3e39490a13f7fd39},

doi = {10.1371/journal.pone.0167426},

issn = {19326203},

year  = {2016},

date = {2016-01-01},

journal = {PLoS ONE},

volume = {11},

number = {11},

publisher = {Public Library of Science},

abstract = {Brachypodium distachyon L. Beauv. (Brachypodium) is a species that has become an excellent model system for gaining a better understanding of various areas of grass biology and improving plant breeding. Although there are some studies of an in vitro Brachypodium culture including somatic embryogenesis, detailed knowledge of the composition of the main cell wall components in the embryogenic callus in this species is missing. Therefore, using the immunocytochemical approach, we targeted 17 different antigens of which five were against the arabinogalactan proteins (AGP), three were against extensins, six recognised pectic epitopes and two recognised hemicelluloses. These studies were complemented by histological and scanning electron microscopy (SEM) analyses. We revealed that the characteristic cell wall components of Brachypodium embryogenic calli are AGP epitopes that are recognised by the JIM16 and LM2 antibodies, an extensin epitope that is recognised by the JIM11 antibody and a pectic epitopes that is recognised by the LM6 antibody. Furthermore, we demonstrated that AGPs and pectins are the components of the extracellular matrix network in Brachypodium embryogenic culture. Additionally, SEM analysis demonstrated the presence of an extracellular matrix on the surface of the calli cells. In conclusion, the chemical compositions of the cell walls and ECMSN of Brachypodium callus show spatial differences that correlate with the embryogenic character of the cells. Thus, the distribution of pectins, AGPs and hemicelluloses can be used as molecular markers of embryogenic cells. The presented data extends the knowledge about the chemical composition of the embryogenic callus cells of Brachypodium. © 2016 Betekhtin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {21},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84985905491,

title = {Integument cell differentiation in dandelions (Taraxacum, Asteraceae, Lactuceae) with special attention paid to plasmodesmata},

author = { B.J. Płachno and E.U. Kurczyńska and P. Świątek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84985905491&doi=10.1007%2fs00709-015-0894-2&partnerID=40&md5=c6e007e4d6cf72f6ef670feff5d4494d},

doi = {10.1007/s00709-015-0894-2},

issn = {0033183X},

year  = {2016},

date = {2016-01-01},

journal = {Protoplasma},

volume = {253},

number = {5},

pages = {1365-1372},

publisher = {Springer-Verlag Wien},

abstract = {The aim of the paper is to determine what happens with plasmodesmata when mucilage is secreted into the periplasmic space in plant cells. Ultrastructural analysis of the periendothelial zone mucilage cells was performed on examples of the ovule tissues of several sexual and apomictic Taraxacum species. The cytoplasm of the periendothelial zone cells was dense, filled by numerous organelles and profiles of rough endoplasmic reticulum and active Golgi dictyosomes with vesicles that contained fibrillar material. At the beginning of the differentiation process of the periendothelial zone, the cells were connected by primary plasmodesmata. However, during the differentiation and the thickening of the cell walls (mucilage deposition), the plasmodesmata become elongated and associated with cytoplasmic bridges. The cytoplasmic bridges may connect the protoplast to the plasmodesmata through the mucilage layers in order to maintain cell-to-cell communication during the differentiation of the periendothelial zone cells. © 2015, The Author(s).},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84977117631,

title = {The fate of surface cell layers of Daucus carota (L.) embryos raised in suspension culture},

author = { I. Dobrowolska and J. Kwaśniewska and P.W. Barlow and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84977117631&doi=10.1080%2f11263504.2014.989288&partnerID=40&md5=8272612f1c7b51f6cc7c9b58ac233cb3},

doi = {10.1080/11263504.2014.989288},

issn = {11263504},

year  = {2016},

date = {2016-01-01},

journal = {Plant Biosystems},

volume = {150},

number = {4},

pages = {622-630},

publisher = {Taylor and Francis Ltd.},

abstract = {The ultrastructure and fate of surface cells covering mature somatic embryos of Daucus carota grown in suspension culture were analyzed and new information obtained concerning somatic embryogenesis in these conditions. Our studies showed that during some developmental stages, these embryos were covered irregularly and discontinuously by cells with a typical protodermal phenotype characterized by a cuticle on the outer cell wall. We observed that cells with cuticles were peeled off from the surface of mature embryos. Before peeling off, these cells underwent programmed cell death, which was confirmed by the TdT-mediated dUTP nick end labeling method. Transmission electron microscopy revealed advanced processes of autophagy in these cells. © 2015 Società Botanica Italiana.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85003012274,

title = {Diverse influence of nanoparticles on plant growth with a particular emphasis on crop plants},

author = { A. Milewska-Hendel and R. Gawecki and M. Zubko and D. Stróż and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85003012274&doi=10.5586%2faa.1694&partnerID=40&md5=bda7fd8ffc68b024bdb5d5b9981b0440},

doi = {10.5586/aa.1694},

issn = {00650951},

year  = {2016},

date = {2016-01-01},

journal = {Acta Agrobotanica},

volume = {69},

number = {4},

publisher = {Polish Botanical Society},

abstract = {The article describes the current knowledge about the impact of nanoparticles on plant development with a particular emphasis on crop plants. Nanotechnology is an intensively developing field of science. This is due to the enormous hopes that have been placed on the achievements of nanotechnology in various areas of life. Increasingly, it has been noted that apart from the future benefits of nanotechnology in our everyday life, nanoparticles (NPs) may also have adverse effects that have not been sufficiently explored and understood. Most analyses to date have been focused on the influence of nanomaterials on the physiological processes primarily in animals, humans and bacteria. Although our knowledge about the influence of NPs on the development of plants is considerably smaller, the current views are presented below. Such knowledge is extremely important since NPs can enter the food chain, which may have an influence on human health. © The Author(s) 2016.},

note = {22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84897584049,

title = {Increased symplasmic permeability in barley root epidermal cells correlates with defects in root hair development},

author = { M. Marzec and A. Muszyńska and M. Melzer and H. Sas-Nowosielska and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897584049&doi=10.1111%2fplb.12066&partnerID=40&md5=8042210e45154415f0b6015e9f0d8c70},

doi = {10.1111/plb.12066},

issn = {14358603},

year  = {2014},

date = {2014-01-01},

journal = {Plant Biology},

volume = {16},

number = {2},

pages = {476-484},

abstract = {It is well known that the process of plant cell differentiation depends on the symplasmic isolation of cells. Before starting the differentiation programme, the individual cell or group of cells should restrict symplasmic communication with neighbouring cells. We tested the symplasmic communication between epidermal cells in the different root zones of parental barley plants Hordeum vulgare L., cv. 'Karat' with normal root hair development, and two root hairless mutants (rhl1.a and rhl1.b). The results clearly show that symplasmic communication was limited during root hair differentiation in the parental variety, whereas in both root hairless mutants epidermal cells were still symplasmically connected in the corresponding root zone. This paper is the first report on the role of symplasmic isolation in barley root cell differentiation, and additionally shows that a disturbance in the restriction of symplasmic communication is present in root hairless mutants. © 2013 German Botanical Society and The Royal Botanical Society of the Netherlands.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84924104311,

title = {Importance of symplasmic communication in cell differentiation},

author = { M. Marzec and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924104311&doi=10.4161%2fpsb.27931&partnerID=40&md5=29cf2dd4378aa903a74d02abd49488e5},

doi = {10.4161/psb.27931},

issn = {15592316},

year  = {2014},

date = {2014-01-01},

journal = {Plant Signaling and Behavior},

volume = {9},

number = {1},

publisher = {Landes Bioscience},

abstract = {Symplasmic communication via plasmodesmata (PD) is part of the system of information exchange between plant cells. Molecules that pass through the PD include ions, some hormones, minerals, amino acids, and sugars but also proteins, transcription factors, and different classes of RNA, and as such PD can participate in the coordination of plant growth and development. This review summarizes the current literature on this subject and the role of PD in signal exchange, the importance of symplasmic communication and symplasmic domains in plant cell differentiation, and highlights the future prospective in the exploration of PD functions in plants. Moreover, this review also describes the potential use of barley root epidermis and non-zygotic embryogenesis in study of symplasmic communication during cell differentiation. © 2014 Landes Bioscience.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84899137346,

title = {Importance of symplasmic communication in cell differentiation},

author = { M. Marzec and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899137346&doi=10.4161%2fpsb.27931tt&partnerID=40&md5=08b24cce230ea4e888f311de5786eb5f},

doi = {10.4161/psb.27931tt},

issn = {15592316},

year  = {2014},

date = {2014-01-01},

journal = {Plant Signaling and Behavior},

volume = {9},

number = {JAN},

publisher = {Landes Bioscience},

abstract = {Symplasmic communication via plasmodesmata (PD) is part of the system of information exchange between plant cells. Molecules that pass through the PD include ions, some hormones, minerals, amino acids, and sugars but also proteins, transcription factors, and different classes of RNA, and as such PD can participate in the coordination of plant growth and development. This review summarizes the current literature on this subject and the role of PD in signal exchange, the importance of symplasmic communication and symplasmic domains in plant cell differentiation, and highlights the future prospective in the exploration of PD functions in plants. Moreover, this review also describes the potential use of barley root epidermis and non-zygotic embryogenesis in study of symplasmic communication during cell differentiation. 00811. © 2014 Landes Bioscience.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84905679687,

title = {Cellular events during interfascicular cambium ontogenesis in inflorescence stems of Arabidopsis},

author = { E. Mazur and E.U. Kurczyńska and J. Friml},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84905679687&doi=10.1007%2fs00709-014-0620-5&partnerID=40&md5=b9a414a86da0390d597a07374c3a6ec4},

doi = {10.1007/s00709-014-0620-5},

issn = {0033183X},

year  = {2014},

date = {2014-01-01},

journal = {Protoplasma},

volume = {251},

number = {5},

pages = {1125-1139},

publisher = {Springer-Verlag Wien},

abstract = {Development of cambium and its activity is important for our knowledge of the mechanism of secondary growth. Arabidopsis thaliana emerges as a good model plant for such a kind of study. Thus, this paper reports on cellular events taking place in the interfascicular regions of inflorescence stems of A. thaliana, leading to the development of interfascicular cambium from differentiated interfascicular parenchyma cells (IPC). These events are as follows: appearance of auxin accumulation, PIN1 gene expression, polar PIN1 protein localization in the basal plasma membrane and periclinal divisions. Distribution of auxin was observed to be higher in differentiating into cambium parenchyma cells compared to cells within the pith and cortex. Expression of PIN1 in IPC was always preceded by auxin accumulation. Basal localization of PIN1 was already established in the cells prior to their periclinal division. These cellular events initiated within parenchyma cells adjacent to the vascular bundles and successively extended from that point towards the middle region of the interfascicular area, located between neighboring vascular bundles. The final consequence of which was the closure of the cambial ring within the stem. Changes in the chemical composition of IPC walls were also detected and included changes of pectic epitopes, xyloglucans (XG) and extensins rich in hydroxyproline (HRGPs). In summary, results presented in this paper describe interfascicular cambium ontogenesis in terms of successive cellular events in the interfascicular regions of inflorescence stems of Arabidopsis. © 2014 Springer-Verlag Wien.},

note = {21},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84899417142,

title = {Plasma membrane and cell wall properties of an aspen hybrid (Populus tremula × tremuloides) parenchyma cells under the influence of salt stress},

author = { A. Muszyńska and K. Jarocka and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899417142&doi=10.1007%2fs11738-014-1490-3&partnerID=40&md5=d1c52f50bc6ed1f8bd174cbd50666031},

doi = {10.1007/s11738-014-1490-3},

issn = {01375881},

year  = {2014},

date = {2014-01-01},

journal = {Acta Physiologiae Plantarum},

volume = {36},

number = {5},

pages = {1155-1165},

publisher = {Polish Academy of Sciences},

abstract = {The effect of salinity on cell turgor, plasma membrane permeability and cell wall elasticity has been measured in petioles of an aspen hybrid using the cell pressure probe. Control plants were grown in soil without the addition of NaCl and treated plants were grown in soil with 50 mM of NaCl for 1, 2, 3 and 4 weeks. In parenchyma cells from Populus tremula × tremuloides petioles with an increased level of NaCl in the soil: (a) turgor pressure was reduced after 1 week of treatment but afterward it was similar to untreated plants, (b) the value of elastic modulus of the cell walls increased, and (c) hydraulic conductivity of the plasma membrane of treated plants decreased in comparison to untreated ones. No histological differences and distribution of JIM5 antibody between the petioles of plants grown under salinity and the untreated were found. In cell walls of parenchyma and collenchyma from plants grown under salinity, the presence of pectic epitopes recognized by JIM7 antibodies was increased in comparison to the control plants. The obtained results indicate that under salt stress the permeability of water through plasma membrane is disturbed, cell walls became more rigid but the turgor pressure did not change. © 2014 The Author(s).},

note = {21},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84879842940,

title = {Spatio-temporal distribution and methyl-esterification of pectic epitopes provide evidence of developmental regulation of pectins during somatic embryogenesis in Arabidopsis thaliana},

author = { K. Sala and I.W. Potocka and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84879842940&doi=10.1007%2fs10535-013-0304-6&partnerID=40&md5=6d23dd96351eb5038e6f5123ed9959f0},

doi = {10.1007/s10535-013-0304-6},

issn = {00063134},

year  = {2013},

date = {2013-01-01},

journal = {Biologia Plantarum},

volume = {57},

number = {3},

pages = {410-416},

abstract = {The aim of the present study was to describe the occurrence of three pectic epitopes, recognized by JIM7, LM19, and LM5 antibodies, during somatic (SE) and zygotic (ZE) embryogenesis in Arabidopsis thaliana. The epitopes recognized by JIM7 and LM19 antibodies showed different distributions during SE stages. Moreover, in the early stages of somatic embryo development, a cytoplasmic occurrence of LM19 epitope was detected. Distribution of a pectic epitope recognized by LM5 antibody corresponded to a vascular system differentiation pattern. Occurrence of LM5 epitope was the same in both zygotic and somatic embryos and often restricted to newly synthesized walls of two adjacent cells. These data suggest that both low and high methyl-esterified pectins (recognized by LM19 and JIM7 antibodies; respectively) are developmentally regulated during SE stages and (1→4)-β-D-galactan epitope (recognized by LM5 antibody) may play a role in cell cytokinesis. © 2013 Springer Science+Business Media Dordrecht.},

note = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84867669808,

title = {Distribution of lipid transfer protein 1 (LTP1) epitopes associated with morphogenic events during somatic embryogenesis of Arabidopsis thaliana},

author = { I.W. Potocka and T.C. Baldwin and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867669808&doi=10.1007%2fs00299-012-1314-0&partnerID=40&md5=fcae8a1ea519f971e385aba750e03a90},

doi = {10.1007/s00299-012-1314-0},

issn = {07217714},

year  = {2012},

date = {2012-01-01},

journal = {Plant Cell Reports},

volume = {31},

number = {11},

pages = {2031-2045},

abstract = {Using immunocytochemical methods, at both the light and electron microscopic level, we have investigated the spatial and temporal distribution of lipid transfer protein 1 (LTP1) epitopes during the induction of somatic embryogenesis in explants of Arabidopsis thaliana. Immunofluorescence labelling demonstrated the presence of high levels of LTP1 epitopes within the proximal regions of the cotyledons (embryogenic regions) associated with particular morphogenetic events, including intense cell division activity, cotyledon swelling, cell loosening and callus formation. Precise analysis of the signal localization in protodermal and subprotodermal cells indicated that cells exhibiting features typical of embryogenic cells were strongly labelled, both in walls and the cytoplasm, while in the majority of meristematic-like cells no signal was observed. Staining with lipophilic dyes revealed a correlation between the distribution of LTP1 epitopes and lipid substances within the cell wall. Differences in label abundance and distribution between embryogenic and non-embryogenic regions of explants were studied in detail with the use of immunogold electron microscopy. The labelling was strongest in both the outer periclinal and anticlinal walls of the adaxial, protodermal cells of the proximal region of the cotyledon. The putative role(s) of lipid transfer proteins in the formation of lipid lamellae and in cell differentiation are discussed. Key message Occurrence of lipid transfer protein 1 epitopes in Arabidopsis explant cells accompanies changes in cell fate and may be correlated with the deposition of lipid substances in the cell walls. © 2012 The Author(s).},

note = {31},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85027954411,

title = {Expression of the BBM gene during somatic embryogenesis of Arabidopsis thaliana},

author = { K. Kulińska-Łukaszek and M. Tobojka and A. Adamiok and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027954411&doi=10.1007%2fs10535-012-0105-3&partnerID=40&md5=a12819ff91126b147bbc867a6fe6a9e3},

doi = {10.1007/s10535-012-0105-3},

issn = {00063134},

year  = {2012},

date = {2012-01-01},

journal = {Biologia Plantarum},

volume = {56},

number = {2},

pages = {389-394},

publisher = {Kluwer Academic Publishers},

abstract = {The relationship between somatic embryogenesis (SE) and the expression of the BABY BOOM (BBM) gene was studied in cultured immature zygotic embryos (IZEs) using a transgenic line of Arabidopsis thaliana containing a BBMPro::GUS construct. Results showed spatio-temporal differences in BBM expression in explants during culture. BBM promoter activity was observed in freshly isolated IZEs except distal parts of cotyledons. At the beginning of culture, considerable increase of GUS staining intensity was observed in all parts of explants, which maintained at high level over next few days and coincide with cell divisions. Gradual decrease of GUS distribution in explants was observed at about the 5th day of culture. BBM promoter activity became largely restricted to dividing cells, then to developing somatic embryos, shoot-like structures and callus. In parts of explants not involved in morphogenesis BBM promoter activity was absent or hardly seen. Thus the in vitro expression of BBM coincides with cell proliferation and morphogenesis. © 2012 Springer Science+Business Media B.V.},

note = {33},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84855334890,

title = {Rays, intrusive growth, and storied cambium in the inflorescence stems of Arabidopsis thaliana (L.) Heynh},

author = { E. Mazur and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855334890&doi=10.1007%2fs00709-011-0266-5&partnerID=40&md5=c34e5fceb33acc503bd926c670d8bf8b},

doi = {10.1007/s00709-011-0266-5},

issn = {0033183X},

year  = {2012},

date = {2012-01-01},

journal = {Protoplasma},

volume = {249},

number = {1},

pages = {217-220},

abstract = {Arabidopsis thaliana is a model plant used in analysis of different aspects of plant growth and development. Under suitable conditions, secondary growth takes place in the hypocotyl of Arabidopsis plants, a finding which helps in understanding many aspects of xylogenesis. However, not all developmental processes of secondary tissue can be studied here, as no secondary rays and intrusive growth have been detected in hypocotyl. However, results presented here concerning the secondary growth in inflorescence stems of Arabidopsis shows that both secondary rays and intrusive growth of cambial cells can be detected, and that, in the interfascicular regions, a storied cambium can be developed. © 2011 The Author(s).},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84855334639,

title = {Differences in protodermal cell wall structure in zygotic and somatic embryos of Daucus carota (L.) cultured on solid and in liquid media},

author = { I. Dobrowolska and O. Majchrzak and T.C. Baldwin and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855334639&doi=10.1007%2fs00709-011-0268-3&partnerID=40&md5=4f158c10e7cb0cd036ff04b24c03dc62},

doi = {10.1007/s00709-011-0268-3},

issn = {0033183X},

year  = {2012},

date = {2012-01-01},

journal = {Protoplasma},

volume = {249},

number = {1},

pages = {117-129},

abstract = {The ultrastructure, cuticle, and distribution of pectic epitopes in outer periclinal walls of protodermal cells of Daucus carota zygotic and somatic embryos from solid and suspension culture were investigated. Lipid substances were present as a continuous layer in zygotic and somatic embryos cultured on solid medium. Somatic embryos from suspension cultures were devoid of cuticle. The ultrastructure of the outer walls of protodermis of embryos was similar in zygotic and somatic embryos from solid culture. Fibrillar material was observed on the surface of somatic embryos. In zygotic embryos, in cotyledons and root pectic epitopes recognised by the antibody JIM5 were observed in all cell walls. In hypocotyls of these embryos, these pectic epitopes were not present in the outer periclinal and anticlinal walls of the protodermis. In somatic embryos from solid media, distribution of pectic epitopes recognised by JIM5 was similar to that described for their zygotic counterparts. In somatic embryos from suspension culture, pectic epitopes recognised by JIM5 were detected in all cell walls. In the cotyledons and hypocotyls, a punctate signal was observed on the outside of the protodermis. Pectic epitopes recognised by JIM7 were present in all cell walls independent of embryo organs. In zygotic embryos, this signal was punctate; in somatic embryos from both cultures, this signal was uniformly distributed. In embryos from suspension cultures, a punctate signal was detected outside the surface of cotyledon and hypocotyl. These data are discussed in light of current models for embryogenesis and the influence of culture conditions on cell wall structure. © 2011 The Author(s).},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79954415969,

title = {Histology and symplasmic tracer distribution during development of barley androgenic embryos},

author = { J. Wróbel-Marek and P.W. Barlow and K. Gorka and D. Nabialkowska and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79954415969&doi=10.1007%2fs00425-010-1345-0&partnerID=40&md5=91bf7dc3facdfc99067d2fbffdc8b59d},

doi = {10.1007/s00425-010-1345-0},

issn = {00320935},

year  = {2011},

date = {2011-01-01},

journal = {Planta},

volume = {233},

number = {5},

pages = {873-881},

abstract = {The present study concerns three aspects of barley androgenesis: (1) the morphology and histology of the embryos during their development, (2) the time course of fluorescent symplasmic tracers' distribution, and (3) the correlation between symplasmic communication and cell differentiation. The results indicate that barley embryos, which are developing via an androgenic pathway, resemble their zygotic counterparts with respect to their developmental stages, morphology and histology. Analysis of the distribution of the symplasmic tracers, HPTS, and uncaged fluorescein indicates the symplasmic isolation of (1) the protodermis from the underlying cells of the late globular stage onwards, and (2) the embryonic organs at the mature stage of development. © 2011 The Author(s).},

note = {20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34447121906,

title = {Histological analysis of direct somatic embryogenesis in Arabidopsis thaliana (L.) Heynh},

author = { E.U. Kurczyńska and M.D. Gaj and A. Ujczak and E. Mazur},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34447121906&doi=10.1007%2fs00425-007-0510-6&partnerID=40&md5=3d00a2a2cebccc7fda9ee11b0da3e024},

doi = {10.1007/s00425-007-0510-6},

issn = {00320935},

year  = {2007},

date = {2007-01-01},

journal = {Planta},

volume = {226},

number = {3},

pages = {619-628},

abstract = {In Arabidopsis the in vitro culture of immature zygotic embryos (IZEs) at a late stage of development, on the solid medium containing synthetic auxin, leads to formation of somatic embryos via direct somatic embryogenesis (DSE). The presented results provide evidence that in IZE cells competent for DSE are located in the protodermis and subprotodermis of the adaxial side of cotyledons and somatic embryos displayed a single- or multicellular origin. Transgenic Arabidopsis lines expressing the GUS reporter gene, driven by the DR5 and LEC2 promoters, were used to analyse the distribution of auxin to mark embryogenic cells in cultured explants and develop somatic embryos. The analysis showed that at the start of the culture auxin was accumulated in all explant tissues, but from the fourth day onwards its location shifted to the protodermis and subprotodermis of the explant cotyledons. In globular somatic embryos auxin was detected in all cells, with a higher concentration in the protodermis, and in the heart stage its activity was mainly displayed in the shoot, root pole and cotyledon primordia. The embryogenic nature of dividing protodermal and subprotodermal cells accumulating auxin was confirmed by high expression of promoter activity of LEC2 in these cells. Analysis of symplasmic tracer (CFDA) distribution indicated symplasmic isolation between tissues engaged in DSE and other parts of an explant. Symplasmic isolation of somatic embryos from the explant was also detected. © 2007 Springer-Verlag.},

note = {93},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33947263587,

title = {The anatomy of the chi-chi of Ginkgo biloba suggests a mode of elongation growth that is an alternative to growth driven by an apical meristem},

author = { P.W. Barlow and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33947263587&doi=10.1007%2fs10265-006-0050-3&partnerID=40&md5=c03c232b792411a8507ea7f57573a5ce},

doi = {10.1007/s10265-006-0050-3},

issn = {09189440},

year  = {2007},

date = {2007-01-01},

journal = {Journal of Plant Research},

volume = {120},

number = {2},

pages = {269-280},

abstract = {The chi-chi of Ginkgo biloba L. are cylindrical woody structures that grow downwards from the branches and trunks of old trees, eventually entering the soil where they give rise to adventitious shoots and roots. Examination of segments of young chi-chi taken from a mature ginkgo tree revealed an internal woody portion with irregular growth rings of tracheid-containing secondary xylem covered by a vascular cambium and bark. The cambium was composed of both fusiform cells and parenchymatous ray cells. Near the tip of the chi-chi, these two types of cambial cells had orientations ranging between axial, radial and circumferential with respect to the cylindrical form of the chi-chi. The xylem rays and tracheids that derived from the cambium showed correspondingly variable orientations. Towards the base of the chi-chi, the fusiform cells and young tracheids were aligned parallel to the axis, indicating that the orientation of the cambial cells in basal regions of the chi-chi gradually became normalised as the tip of the chi-chi extended forwards. Nevertheless, in such basal sites, tracheids near the centre of the chi-chi showed variable orientations in accordance with their mode of formation during the early stages of chi-chi development. The initiation of a chi-chi is proposed to derive from a localised hyperactivity of vascular cambial-cell production in the supporting stem. The chi-chi elongates by tip growth, but it does so in a manner different from organ growth driven by an apical meristem. It is suggested that the chi-chi of Ginkgo is an "evolutionary experiment" that makes use of the vascular cambium, not only for its widening growth but also for its elongation. © The Botanical Society of Japan and Springer 2007.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0242543236,

title = {Perception of gravity expressed by production of cambial callus in ash (Fraxinus excelsior L.) internodes},

author = { E.U. Kurczyńska and Z. Hejnowicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0242543236&partnerID=40&md5=c43ff295b2896330f0568cc568b167fd},

issn = {00016977},

year  = {2003},

date = {2003-01-01},

journal = {Acta Societatis Botanicorum Poloniae},

volume = {72},

number = {3},

pages = {207-211},

publisher = {Polish Botanical Society},

abstract = {In ash internodes isolated from vertical stems during winter dormancy, cambial activity was stimulated by applying an aqueous solution of auxin to the apical end and water to the basal end. The internodes maintained nearly horizontally produced cambial callus at the apical cut surface with more callus on the upper half although both halves were in contact with the same concentration of auxin. A differential response to auxin of the cambium in the upper and lower halves of the horizontally oriented internodes is postulated.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-52449119574,

title = {Anatomy of abscission zone of Betula pendula (Roth.) leaves from trees growing under different levels of pollution},

author = { I. Dobrowolska and E.U. Kurczyńska and W. Dmuchowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-52449119574&partnerID=40&md5=fe391ead6ea674355d76f00620695645},

issn = {11357606},

year  = {2002},

date = {2002-01-01},

journal = {Cuadernos de Medicina Forense},

number = {28},

pages = {13-19},

abstract = {A study was carried out on the leaf abscission zone from birch trees growing on polluted sites (two) and a non-polluted site (one). Samples for anatomical investigation were collected from six trees on each site, during three succeeding vegetation seasons. It was observed that in trees growing at the polluted sites: 1) maturation of the abscission zone was faster, 2) the protective layer was thinner and 3) the formation of leaf scar periderm was delayed in comparison with trees from the non-polluted site. The results obtained suggest that environmental pollution influences the formation of the abscission zone and the protection of the leaf scar.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-52549113262,

title = {Anatomy of abscission zone of betula pendula (Roth.) leaves from trees growing under different levels of pollution},

author = { I. Dobrowolska and E.U. Kurczyńska and W. Dmuchowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-52549113262&partnerID=40&md5=ea13dfeb3b8ceba425bf0c7c5fadb9aa},

issn = {16411307},

year  = {2001},

date = {2001-01-01},

journal = {Dendrobiology},

volume = {46},

pages = {13-19},

publisher = {Polska Akademia Nauk},

abstract = {A study was carried out on the leaf abscission zone from birch trees growing on polluted sites (two) and a non-polluted site (one). Samples for anatomical investigation were collected from six trees on each site, during three succeeding vegetation seasons. It was observed that in trees growing at the polluted sites: 1) maturation of the abscission zone was faster, 2) the protective layer was thinner and 3) the formation of leaf scar periderm was delayed in comparison with trees from the non-polluted site. The results obtained suggest that environmental pollution influences the formation of the abscission zone and the protection of the leaf scar.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33748510311,

title = {Physiological activity of ectomycorrhizas in a moderately polluted forest (Ratanica catchment, southern Poland)},

author = { B. Kieliszewska-Rokicka and E.U. Kurczyńska and T. Leski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748510311&partnerID=40&md5=1e603e1f6d991afa63b4e00419045a51},

issn = {16411307},

year  = {2000},

date = {2000-01-01},

journal = {Dendrobiology},

volume = {45},

pages = {47-59},

abstract = {Ectomycorrhizas of Scots pine (Pinus sylvestris L.) and beech (Fagus sylvatica L.) were sampled in a mature forest ecosystem exposed for more than 40 years to moderate levels of gaseous and dust pollutants. Soil of the forest site was characterised by low pH and accumulation of heavy metals (Pb, Mn, Zn, Cu, Cd, and Fe). Mycorrhizal vitality and enzyme activity of the root-surface and soil acid phosphatase (AcPase) were studied at 17 measurement points (0-5 cm soil depth) in relation to the content of inorganic phosphate (P2O5) and aluminium ions (Al3+) in soil. The concentration of essential nutrients (C, N, P, Ca, Mg) and the ratios Ca/Al, Mg/Al and N/P were analysed in fine roots. High concentration of Al3+ in soil (40-118 meq kg-1) and low level of Pi (12-44 mg P2O5 kg-1) were accompanied by high activity of the root-surface AcPase of pine and beech mycorrhizas (25-67 and 3386 μmol pNP g-1 fresh weight h-1, respectively) and soil AcPase (6.8-22.4 μmol pNP g-1 dry weight h-1). The results indicate that tree fine roots are undoubtedly under stress that is evidenced by a disturbance in P uptake and accumulation. However, the high vitality mycorrhizas and high Ca/Al ratio in fine roots suggest, that the defence mechanisms of mycorrhizas and the rhizosphere are still able to ameliorate the influence of anthropogenic pollution.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0344589335,

title = {Influence of ozone and soil nitrogen content on the stem anatomy of Norway spruce saplings grown in open-top chambers},

author = { E.U. Kurczyńska and A. Bastrup-Birk and L. Mortensen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0344589335&doi=10.1016%2fS0098-8472%2898%2900025-2&partnerID=40&md5=733dd3361a927c56c8ec56208af6faf6},

doi = {10.1016/S0098-8472(98)00025-2},

issn = {00988472},

year  = {1998},

date = {1998-01-01},

journal = {Environmental and Experimental Botany},

volume = {40},

number = {2},

pages = {113-121},

abstract = {Four-year-old Norway spruce saplings (Picea abies) were exposed to different concentrations of ozone in open-top chambers for 22 weeks. The treatments were charcoal filtered air (CF), non-filtered ambient air (NF) and two elevated ozone treatments: non-filtered air with O3 added at 25 nl l-1 (NF25) and at 50 nl l-1 (NF50). In each chamber, half of the investigated saplings were grown on nitrogen-poor soil (17 mg N kg-1) and half on nitrogen-enriched soil (34 mg N kg-1). Changes in the anatomy of the stem, the growth parameters, and nitrogen concentration in needles and stem were followed over one growing season. In saplings grown on nitrogen-enriched soil the number of tracheids and the number of latewood tracheids decreased, but diameter of latewood tracheids increased with increasing ozone concentration in comparison to ambient level of ozone. In the fumigated saplings grown on nitrogen-poor soil, the number of sieve cells and diameter of latewood tracheids decreased with increasing ozone concentration in comparison to ambient level of ozone. Ozone increased the thickness of sieve cell walls independent of the nitrogen level in the soil. Needle length was the only growth parameter that was very little reduced after one growing season of combined nitrogen-enriched soil and fumigation with high ozone concentrations. Foliar and stem nitrogen concentrations did not respond significantly to increased ozone levels.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0345451538,

title = {The influence of air pollutants on needles and stems of Scots pine (Pinus sylvestris L.) trees},

author = { E.U. Kurczyńska and W. Dmuchowski and W. Włoch and A. Bytnerowicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0345451538&doi=10.1016%2fS0269-7491%2897%2900141-3&partnerID=40&md5=879e3c66918167da8c65164c9b78e824},

doi = {10.1016/S0269-7491(97)00141-3},

issn = {02697491},

year  = {1997},

date = {1997-01-01},

journal = {Environmental Pollution},

volume = {98},

number = {3},

pages = {325-334},

abstract = {The influence of air pollution on the chemical composition of needles and stem anatomy (cambium, phloem, and xylem) of Scots pines (Pinus sylvestris L.) was examined in polluted and control forest sites near Warsaw, Poland. Foliar chemical composition was determined as an indicator of contamination of the trees examined anatomically. Anatomical analysis was carried out on samples including phloem, cambium, and current-year xylem collected at the breast height and from the tree top of the leader during one growing season. At the control site in current-year foliage, concentrations were N 1.53%, P 0.18%, K 0.5%, Ca 0.17%, Mg 910 ppm, Na 45 ppm, and S 0.145%, while at the polluted site concentrations were N 1.73%, P 0.18%, K 0.66%, Ca 0.35%, Mg 1509 ppm, Na 105 ppm and S 0.201%. Concentrations of heavy metals were significantly higher at the polluted site; only Mn was found in lower concentration. In trees from the polluted site, anatomical analysis revealed a decreased number of cambial cells at the beginning of growing season, phloem cells (at the tree top average number in a radial file was 21 and 14 in control and polluted sites, respectively), and formation of false rings with tracheids, similar to those in compression wood. Anatomical changes observed in trees from polluted sites are most likely caused by toxic effects of pollutants.},

note = {66},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0030186842,

title = {Morphological and anatomical changes of scots pine dwarf shoots induced by air pollutants},

author = { E.U. Kurczyńska and M. Bełtowski and W. Włoch},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030186842&doi=10.1016%2f0098-8472%2896%2901005-2&partnerID=40&md5=fcd2e84ff4223d090e1504987c01b84c},

doi = {10.1016/0098-8472(96)01005-2},

issn = {00988472},

year  = {1996},

date = {1996-01-01},

journal = {Environmental and Experimental Botany},

volume = {36},

number = {2},

pages = {185-197},

publisher = {Elsevier B.V.},

abstract = {This study refers to the observation of Leyton and Juniper, that the Scots pine needle bases surrounded by multiple layers of dead scales can absorb water from dew or mist. It is suggested that this ability provides an additional site of entry for toxins originating from air pollutants. In connection with this hypothesis investigations were carried out on the dwarf shoots of Pinus sylvestris L. trees growing in different areas along an air pollution gradient. The damages to the needles, the 'weathering' of the sheath scale surrounding the base of the dwarf shoots, and the decay of surface cells in the basal part of the shoot apex in the axil of the needles and on the adaxial surface of the needles were measured. All of them were exacerbated by increased pollution.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84988353962,

title = {Vessel differentiation in isolated stem segments of Fraxinus excelsior L. After treatment with auxin},

author = { E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84988353962&doi=10.5586%2fasbp.1992.030&partnerID=40&md5=7bef47e0f80ce59cc33caad115d5281e},

doi = {10.5586/asbp.1992.030},

issn = {00016977},

year  = {1992},

date = {1992-01-01},

journal = {Acta Societatis Botanicorum Poloniae},

volume = {61},

number = {3-4},

pages = {343-357},

publisher = {Polskie Towarzystwo Botaniczne},

abstract = {Experiments were carried out on isolated stem segments of ash collected from natural conditions during the winter dormancy season. In order to stimulate cambial activity and vessel differentiation the internodes were treated with auxin. The duration of treatment with auxin was different in the particular experimental variants. It appeared that auxin was necessary not only for induction of vessel differentiation but also during their maturation. The results are discussed on the basis of S ac h s∗ (1981) hypothesis.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84989742233,

title = {Differentiation of circular vessels in isolated segments of Fraxinus excelsior},

author = { E.U. Kurczyńska and Z. Hejnowicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84989742233&doi=10.1111%2fj.1399-3054.1991.tb02153.x&partnerID=40&md5=d9a1f1aa2247d664b6ae9ce9c4dfaee8},

doi = {10.1111/j.1399-3054.1991.tb02153.x},

issn = {00319317},

year  = {1991},

date = {1991-01-01},

journal = {Physiologia Plantarum},

volume = {83},

number = {2},

pages = {275-280},

abstract = {The differentiation of circular vessels was studied in stem segments which had passed winter dormancy and were stimulated by application of auxin in vitro. The circular vessels differentiated near the basal end of internodes. The prevailing form of circular vessels was a pair of cells connected by two perforations. Two‐cell vessels never had only one perforation. The occurrence of two perforations strongly supports the view that auxin flux is a stimulus for vessel differentiation and not the very presence or concentration of auxin; the circular vessels developed as a results of a circular flux. In general, the circular vessels appeared in contact with rays. Their formation was delayed in comparison with that of normal vessels. Copyright © 1991, Wiley Blackwell. All rights reserved},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84990059339,

title = {Terminal vessel and early vessel arrangement in internodes of fraxinus excelsior},

author = { E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84990059339&doi=10.5586%2fasbp.1986.001&partnerID=40&md5=56a5853f737236748fa6bf14f40ee84c},

doi = {10.5586/asbp.1986.001},

issn = {00016977},

year  = {1986},

date = {1986-01-01},

journal = {Acta Societatis Botanicorum Poloniae},

volume = {55},

number = {1},

pages = {3-10},

publisher = {Polskie Towarzystwo Botaniczne},

abstract = {Application of auxin to the apical end and to the transverse incision in a dissected internode. led to the formation of early vessels in such a way that the vessels below the incision were aligned with the vessels above it. There was a structural relation between the early vessels and terminal vessels based on the fact that the former overlay the latter. A hypothesis is forwarded stating that a template according to which the early vessels find their positions, exists on the surface of terminal wood.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}