@article{2-s2.0-85152427514,

title = {Nanoparticles coated by chloramphenicol in hydrogels as a useful tool to increase the antibiotic release and antibacterial activity in dermal drug delivery},

author = { D. Bursy and R.J. Balwierz and P. Groch and P. Biernat and A. Byrski and K. Kasperkiewicz and W. Ochȩdzan-Siodłak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85152427514&doi=10.1007%2fs43440-023-00482-4&partnerID=40&md5=dc85cc7fc4892fd338f5291633d78065},

doi = {10.1007/s43440-023-00482-4},

issn = {17341140},

year  = {2023},

date = {2023-01-01},

journal = {Pharmacological Reports},

volume = {75},

number = {3},

pages = {657-670},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {Background: Nanocarriers for antibacterial drugs became hopeful tools against the increasing resistance of bacteria to antibiotics. This work focuses on a comprehensive study of the applicability and therapeutic suitability of dermal carbopol-based hydrogels containing chloramphenicol carried by various nanoparticles (AuNPs and SiNPs). Methods: The different forms of carbopol-based drugs for dermal use were obtained. Five different concentrations of chloramphenicol and two types of nanoparticles (silica and gold) in carbopol-based ointments were tested. The influence of different carbopol formulations with nanocarriers on the rheological properties as well as the release profile of active substances and bacteriostatic activity on five reference strains were determined. Results: The properties of the obtained hydrogels were compared to a commercial formulation, and finally it was possible to obtain a formulation that allowed improved antimicrobial activity over a commercially available detreomycin ointment while reducing the concentration of the antibiotic. Conclusion: The work indicates that it is possible to reduce the concentration of chloramphenicol by four times while maintaining its bacteriostatic activity, which can improve the patient’s safety profile while increasing the effectiveness of the therapy. Graphical abstract: [Figure not available: see fulltext.] © 2023, The Author(s).},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85152414271,

title = {Native Bacteria Isolated from Phosphate Deposits Reveal Efficient Metal Biosorption and Adhesion to Ore Particles},

author = { H. Rabia and M. Ould Hamou and K. Kasperkiewicz and T. Krzykawski and M. Malicka and I.W. Potocka and I. Bodnaruk and A. Merchichi and M. Skowronek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85152414271&doi=10.3390%2fmin13030388&partnerID=40&md5=1bfaf6d1a995b0e8847e053403d2f345},

doi = {10.3390/min13030388},

issn = {2075163X},

year  = {2023},

date = {2023-01-01},

journal = {Minerals},

volume = {13},

number = {3},

publisher = {MDPI},

abstract = {Mining and processing phosphate ore are among the essential branches of the economy in some developing countries, including Algeria. Conventional ore beneficiation methods can harm the environment by consuming tremendous amounts of water resources (during washing and flotation), potentially hazardous chemicals, and thermal energy. Mine water contains toxic metals that, when released, interfere with environmental functioning. Therefore, in line with environmental needs, conventional methods should be gradually replaced with safe biotechnological processes. This study aimed to investigate the biosorption and adhesion abilities of native microorganisms isolated from Djebel Onk ore (Algeria). The examined bacterial strains differed in their metal accumulation efficiency. The incubation of phosphate ore with the native strain Bacillus HK4 significantly increased the recovery of Mg and Cd (at pH 7; 8147.00 and 100.89 µg/g−1; respectively). The HK4 strain also revealed better adhesion to the ore particles than the reference strain of Bacillus subtilis. Thus, biosorption could be more effective when using the native HK4 strain, which can remove Cd and/or Mg over a pH 4–10 range. Moreover, concerning the unique adhesion capacity of HK4, the strain can be considered in the design of bioflotation methods, as well as in the development of an eco-friendly method of ore and post-flotation waste beneficiation. © 2023 by the authors.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85149460033,

title = {Structure of the lipopolysaccharide O-antigen of endophytic Pseudomonas sp. strain L1},

author = { A. Choma and Z. Kaczyński and I. Komaniecka and A. Swatek and K. Kasperkiewicz and M. Pawlik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149460033&doi=10.1016%2fj.carres.2023.108779&partnerID=40&md5=1de182885720d739852b026656a7d36f},

doi = {10.1016/j.carres.2023.108779},

issn = {00086215},

year  = {2023},

date = {2023-01-01},

journal = {Carbohydrate Research},

volume = {525},

publisher = {Elsevier Ltd},

abstract = {The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Pseudomonas sp. Strain L1, the endophytic bacteria of Lolium perenne (ryegrass) plants growing in soil in an industrial area in the Silesia region (Zabrze; Southern Poland). The high-molecular-weight O-PS fraction liberated from Pseudomonas sp. L1 lipopolysaccharide by mild acid hydrolysis was studied using chemical methods, MALDI-TOF mass spectrometry, and 1D and 2D NMR spectroscopy techniques. It was found that the O-specific polysaccharide was built of tetrasaccharide repeating units composed of D-FucpN, D-Fucp4N, and two D-QuipN residues. The following structure of the O-PS of Pseudomonas sp. Strain L1 was established: [Formula presented] © 2023},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85147428219,

title = {Structure of the lipopolysaccharide O-antigen of Serratia spp. strains 10.1WK and 1XS plant endophytes isolated from O. biennis and L. corniculatus},

author = { A. Swatek and Z. Kaczyński and K. Kasperkiewicz and M. Pawlik and I. Komaniecka and A. Choma},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85147428219&doi=10.1016%2fj.carres.2023.108760&partnerID=40&md5=f8d1964e9ef0a34346bd78de7eb5d2ab},

doi = {10.1016/j.carres.2023.108760},

issn = {00086215},

year  = {2023},

date = {2023-01-01},

journal = {Carbohydrate Research},

volume = {524},

publisher = {Elsevier Ltd},

abstract = {O-specific polysaccharides (O-PSs) isolated from lipopolysaccharides of Serratia spp., strains 10.1WK and 1XS, which are endophytic bacteria of Oenothera biennis (common evening-primrose) and Lotus corniculatus (bird's-foot trefoil), plants growing on a petroleum hydrocarbon polluted site in the Silesia region, were investigated. The high-molecular-weight O-PS fractions liberated from lipopolysaccharides by mild acid hydrolysis were studied using chemical methods, MALDI-TOF mass spectrometry, and a set of 1D and 2D NMR spectroscopy techniques. It was found that both O-specific polysaccharides were built of an identical trisaccharide repeating unit composed of D-Rhap and D-Manp residues. The following structure of the O-PSs of Serratia spp. strains 10.1WK and 1XS was established: →4)-α-D-Rhap-(1 → 3)-β-D-Manp-(1 → 4)-β-D-Rhap–(1→ © 2023 Elsevier Ltd},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85181843549,

title = {Characterization of biomaterials with reference to biocompatibility dedicated for patient-specific finger implants},

author = { A. Byrski and M. Kopernik and Ł. Major and K. Kasperkiewicz and M. Dyner and J.M. Lackner and D.B. Lumenta and R. Major},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85181843549&doi=10.37190%2fABB-02156-2022-02&partnerID=40&md5=956d231d299aef90412f0470f20c4225},

doi = {10.37190/ABB-02156-2022-02},

issn = {1509409X},

year  = {2023},

date = {2023-01-01},

journal = {Acta of Bioengineering and Biomechanics},

volume = {25},

number = {1},

pages = {3-17},

publisher = {Institute of Machine Design and Operation},

abstract = {Purpose: The research was focused on determining basic mechanical properties, surface, and phase structure taking into consideration basic cytotoxicity analysis towards human cells. Methods: Biological tests were performed on human C-12302 fibroblasts cells using 3D-printed Ti6Al4V alloy (Ti64), produced by laser-based powder bed fusion (LB-PBF) and Alumina Toughened Zirconia 20 (ATZ20), produced by lithography-based ceramic manufacturing (LCM). Surface modifications included electropolishing and hydroxyapatite or hydroxyapatite/zinc coating. Structure analysis was carried out using a variety of techniques such as X-Ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM), followed by mechanical properties evaluation using nanoindentation testing. Results: Samples subjected to surface modifications showed diversity among surface and phase structure and mechanical properties. However, the cytotoxicity towards tested cells was not significantly higher than the control. Though, a trend was noted among the materials analysed, indicating that HAp/Zn coating on Ti64 and ATZ20 resulted in the best biological performance increasing cell survivability by more than 10%. Conclusions: Hydroxyapatite coating on Ti64 and ATZ20 resulted in the best biological properties. Tested materials are suitable for in vivo toxicity testing. © 2023, Institute of Machine Design and Operation. All rights reserved.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85144260933,

title = {Effects of chronic exposure to cadmium and copper on the proteome profile of hemolymph in false widow spider Steatoda grossa (Theridiidae)},

author = { K. Wiśniewska and M. Siatkowska and P. Komorowski and K. Napieralska and K. Kasperkiewicz and K. Surmiak–Stalmach and G. Wilczek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144260933&doi=10.1016%2fj.ecoenv.2022.114448&partnerID=40&md5=153c3c677737289e0729b73df004f954},

doi = {10.1016/j.ecoenv.2022.114448},

issn = {01476513},

year  = {2023},

date = {2023-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {249},

publisher = {Academic Press},

abstract = {The aim of this study was to evaluate the quantitative and qualitative changes in the proteome of the hemolymph of female Steatoda grossa spiders (Theridiidae) that were chronically exposed to cadmium and copper in food and were additionally immunostimulated (phorbol 12–myristate 13–acetate (PMA); bacterial suspensions: Staphylococcus aureus (G+); Pseudomonas fluorescens (G–). It was found that the expression of nearly 90 proteins was altered in cadmium–intoxicated spiders and more than 60 in copper–exposed individuals. Regardless of the type of metal used; these proteins were mainly overexpressed in the hemolymph of the exposed spiders. On the other hand; immunostimulation did not significantly change the number of proteins with altered expression in metal–intoxicated individuals. Hemocyanin (Hc) was found to be the most abundant of the proteins identified with altered expression. In copper–intoxicated spiders; immunostimulation increased the expression of A-; E–; F–; and G–chain–containing proteins; while in the case of cadmium–intoxicates spiders; it decreased the expression of E– and A–chain–containing Hc and increased the expression of G–chain–containing Hc. Regardless of the type of metal and immunostimulant used; there was an increase in the expression of actin. In addition; cadmium increased the expression of cullin; vimentin; and ceruloplasmin. The changes observed in the expression of hemolymph proteins indicate their protective function in S. grossa (Theridiidae) spiders under conditions of metal exposure. © 2022},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85140074045,

title = {Antimicrobial materials with improved efficacy dedicated to large craniofacial bone defects after tumor resection},

author = { R. Major and M. Surmiak and K. Kasperkiewicz and R. Kaindl and A. Byrski and Ł. Major and G. Russmueller and Do. Moser and M. Kopernik and J.M. Lackner},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140074045&doi=10.1016%2fj.colsurfb.2022.112943&partnerID=40&md5=70563e8308f74b412a66c3cc5dc6e31f},

doi = {10.1016/j.colsurfb.2022.112943},

issn = {09277765},

year  = {2022},

date = {2022-01-01},

journal = {Colloids and Surfaces B: Biointerfaces},

volume = {220},

publisher = {Elsevier B.V.},

abstract = {The research was focused on alternative treatment techniques, separating immediate and long-term reconstruction stages. The work involved development of ceramic materials dedicated to reconstruction of the temporomandibular joint area. They were based on alumina (aluminum oxide) and characterized by varying porosities. A broad spectrum of studies was conducted to test the proposed material and determine its suitability for mandibular reconstruction. They compared the effects of substrate properties of ceramic materials in terms of biocompatibility, microbiology and systemic toxicity in in vivo studies. Finally it was concluded that Alumina LithaLox 350D is best suited for jawbone implants. © 2022 The Authors},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85137844637,

title = {Hemocompatibile Thin Films Assessed under Blood Flow Shear Forces},

author = { R. Major and G. Wilczek and J. Wiecek and M. Gawlikowski and H. Plutecka and K. Kasperkiewicz and M. Kot and M. Pomorska and R. Ostrowski and M. Kopernik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85137844637&doi=10.3390%2fmolecules27175696&partnerID=40&md5=ec553f2cb1e385bb319b67d9dca65767},

doi = {10.3390/molecules27175696},

issn = {14203049},

year  = {2022},

date = {2022-01-01},

journal = {Molecules},

volume = {27},

number = {17},

publisher = {MDPI},

abstract = {The aim of this study was to minimize the risk of life-threatening thromboembolism in the ventricle through the use of a new biomimetic heart valve based on metal–polymer composites. Finite volume element simulations of blood adhesion to the material were carried out, encompassing radial flow and the cone and plane test together with determination of the effect of boundary conditions. Both tilt-disc and bicuspid valves do not have optimized blood flow due to their design based on rigid valve materials (leaflet made of pyrolytic carbon). The main objective was the development of materials with specific properties dedicated to contact with blood. Materials were evaluated by dynamic tests using blood, concentrates, and whole human blood. Hemostability tests under hydrodynamic conditions were related to the mechanical properties of thin-film materials obtained from tribological tests. The quality of the coatings was high enough to avoid damage to the coating even as they were exposed up to maximum loading. Analysis towards blood concentrates of the hydrogenated carbon sample and the nitrogen-doped hydrogenated carbon sample revealed that the interaction of the coating with erythrocytes was the strongest. Hemocompatibility evaluation under hydrodynamic conditions confirmed very good properties of the developed coatings. © 2022 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85132818018,

title = {Outer Membrane Vesicles as Mediators of Plant–Bacterial Interactions},

author = { M. Rudnicka and M. Noszczyńska and M. Malicka and K. Kasperkiewicz and M. Pawlik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85132818018&doi=10.3389%2ffmicb.2022.902181&partnerID=40&md5=46f103a6c4d53db69a0da7d9312fbd41},

doi = {10.3389/fmicb.2022.902181},

issn = {1664302X},

year  = {2022},

date = {2022-01-01},

journal = {Frontiers in Microbiology},

volume = {13},

publisher = {Frontiers Media S.A.},

abstract = {Plants have co-evolved with diverse microorganisms that have developed different mechanisms of direct and indirect interactions with their host. Recently, greater attention has been paid to a direct “message” delivery pathway from bacteria to plants, mediated by the outer membrane vesicles (OMVs). OMVs produced by Gram-negative bacteria play significant roles in multiple interactions with other bacteria within the same community, the environment, and colonized hosts. The combined forces of innovative technologies and experience in the area of plant–bacterial interactions have put pressure on a detailed examination of the OMVs composition, the routes of their delivery to plant cells, and their significance in pathogenesis, protection, and plant growth promotion. This review synthesizes the available knowledge on OMVs in the context of possible mechanisms of interactions between OMVs, bacteria, and plant cells. OMVs are considered to be potential stimulators of the plant immune system, holding potential for application in plant bioprotection. Copyright © 2022 Rudnicka, Noszczyńska, Malicka, Kasperkiewicz, Pawlik and Piotrowska-Seget.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85120968815,

title = {The effect of selected immunostimulants on hemocytes of the false black widow Steatoda grossa (Theridiidae) spiders under chronic exposition to cadmium},

author = { K. Wiśniewska and M.M. Rost-Roszkowska and J. Homa and K. Kasperkiewicz and K. Surmiak-Stalmach and E. Szulińska and G. Wilczek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120968815&doi=10.1016%2fj.cbpc.2021.109221&partnerID=40&md5=a540ad597a3ee7233969b92b35b3305d},

doi = {10.1016/j.cbpc.2021.109221},

issn = {15320456},

year  = {2022},

date = {2022-01-01},

journal = {Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology},

volume = {252},

publisher = {Elsevier Inc.},

abstract = {The aim of this study was to analyze whether, and to what extent, long–term exposure to cadmium, administered in sublethal concentrations by the oral route, caused changes in the immune potential of hemocytes in adult female Steatoda grossa spiders. We used artificial and natural immunostimulants, namely phorbol 12–myristate 13–acetate (PMA) and bacterial cell suspension based on Gram–positive (G+; Staphylococcus aureus) and Gram–negative (G−; Pseudomonas fluorescens) bacteria, to compare the status of hemocytes in nonstimulated individuals and those subjected to immunostimulation. After cadmium exposure, the percentage of small nongranular hemocytes in response to G+ cell suspension and PMA mitogen was decreased. Furthermore, in the cadmium–intoxicated spiders the percentage of plasmatocytes after immunostimulation remained lower compared to the complementary control group. Exposure to cadmium also induced several degenerative changes, including typical apoptotic and necrotic changes, in the analyzed types of cells. Immunostimulation by PMA mitogen and G+ bacterial suspension resulted in an increase in the number of cisterns in the rough endoplasmic reticulum of granulocytes, in both the control group and cadmium–treated individuals. These changes were accompanied with a low level of metallothioneins in hemolymph. Chronic cadmium exposure may significantly weaken the immune defense system of spiders during infections. © 2021 Elsevier Inc.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85139101700,

title = {Antibodies Recognizing Yersinia enterocolitica Lipopolysaccharides of Various Chemotypes in Synovial Fluids from Patients with Juvenile Idiopathic Arthritis},

author = { K. Kasperkiewicz and A.S. Świerzko and M. Michalski and L. Eppa and M. Skurnik and Z. Zuber and M. Cedzyński},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85139101700&doi=10.1155%2f2022%2f9627934&partnerID=40&md5=aa6bdd27ec3a53849ca73e53d054eb85},

doi = {10.1155/2022/9627934},

issn = {23148861},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Immunology Research},

volume = {2022},

publisher = {Hindawi Limited},

abstract = {Yersinia enterocolitica O:3 (YeO3) is considered to be associated with reactive arthritis (ReA), and its lipopolysaccharide (LPS) has been detected in synovial fluids from patients. Interestingly, YeO3 wild-type LPS was processed by host cells, resulting in truncated LPS molecules presenting the core region. Previously, we reported the immunogenicity but not adjuvanticity of YeO3 LPSs of wild (S) type, Ra, Rd, or Re chemotypes in mice. Here, we demonstrate the presence of YeO3 LPS chemotype-specific antibodies in all analyzed synovial fluids (SF) from patients with juvenile idiopathic arthritis (JIA). Interestingly, the high titer of antibodies specific for the Kdo-lipid A region was found in most tested SF. In contrast, only a few were positive for antibodies recognizing O-specific polysaccharides. Western blot analysis revealed the presence of antibodies reacting with fast-migrating LPS fractions and enterobacterial common antigen (ECA) in synovial fluid samples. Our data also suggest the importance of LPS-associated ECA for the antigenicity of endotoxin. Furthermore, we confirmed in vitro that Yersinia LPS processing leads to the exposure of its core region and enhanced potency of complement lectin pathway activation. © 2022 Katarzyna Kasperkiewicz et al.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85107423588,

title = {Antibacterial optimization of highly deformed titanium alloys for spinal implants},

author = { K. Kasperkiewicz and R. Major and A. Sypien and M. Kot and M. Dyner and Ł. Major and A. Byrski and M. Kopernik and J.M. Lackner},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107423588&doi=10.3390%2fmolecules26113145&partnerID=40&md5=38a80f80c8f5cca6e5674ad29003278f},

doi = {10.3390/molecules26113145},

issn = {14203049},

year  = {2021},

date = {2021-01-01},

journal = {Molecules},

volume = {26},

number = {11},

publisher = {MDPI AG},

abstract = {The goal of the work was to develop materials dedicated to spine surgery that minimized the potential for infection originating from the transfer of bacteria during long surgeries. The bacteria form biofilms, causing implant loosening, pain and finally, a risk of paralysis for patients. Our strategy focused both on improvement of antibacterial properties against bacteria adhesion and on wear and corrosion resistance of tools for spine surgery. Further, a ~35% decrease in implant and tool dimensions was expected by introducing ultrahigh‐strength titanium alloys for less‐invasive surgeries. The tested materials, in the form of thin, multi‐layered coatings, showed nanocrystalline microstructures. Performed direct‐cytotoxicity studies (including lactate dehydrogenase activity measurement) showed that there was a low probability of adverse effects on surrounding SAOS‐2 (Homo sapiens bone osteosarcoma) cells. The microbiological studies (e.g.; ISO 22196 contact tests) showed that implanting Ag nanoparticles into Ti/TixN coatings inhibited the growth of E. coli and S. aureus cells and reduced their adhesion to the material surface. These findings suggest that Ag-nanoparticles present in implant coatings may potentially minimize infection risk and lower inherent stress. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85084267601,

title = {Patient specific implants for jawbone reconstruction after tumor resection},

author = { R. Major and P. Kowalczyk and M. Surmiak and I. Łojszczyk and R. Podgórski and P. Trzaskowska and T. Ciach and G. Russmueller and K. Kasperkiewicz and Ł. Major and R. Jabłoński and J. Kropiwnicki and J.M. Lackner},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084267601&doi=10.1016%2fj.colsurfb.2020.111056&partnerID=40&md5=253408641f21845b02a94f238d9be6fe},

doi = {10.1016/j.colsurfb.2020.111056},

issn = {09277765},

year  = {2020},

date = {2020-01-01},

journal = {Colloids and Surfaces B: Biointerfaces},

volume = {193},

publisher = {Elsevier B.V.},

abstract = {In case of benign and malignant tumours affecting the maxillofacial region, the resection of jawbone reflects the standard therapy in more than 5.000 cases per year within the European Union. The resulting large bone defects lead to scarred, mangled facial appearance, loss of mastication and probably speech, requiring aesthetic and functional surgery as a basis for physical and physiological rehabilitation. Although autologous vascularized bone autografts reflect the current golden standard, the portion of bone available for the procedure is limited and subsequent high-dose anti-cancer chemo-/radiotherapy can lead to local tissue necrosis. Autologous vascularized bone from fibular or iliac-crest autografts is current golden standard in jawbone resection post-treatment, however, the portion of transplantable bone is limited and subsequent high-dose anti-cancer chemo-/radiotherapy often results in tissue necrosis Our research focuses on alternative treatment techniques: tissue reconstruction via novel patient-specifically manufactured maxillofacial implant that stimulates bone tissue growth. The planned neoformation of vascularized bone in such implants within the patient's own body as “bioreactor” is the safest approach in tissue engineering. The works described herein included the design of the metallic substrate of the implant with the use of computed tomography basing on real patients scans and then 3D-printing the substrates from the Ti6Al7Nb powder. The metal core was then evaluated in terms of structural characteristic, cytotoxicity and gene expression through the in vitro tests. Further experiments were focused on fabrication of the biocompatible coating for outer surface of the bone implant that would enhance the healing process and accelerate the tissue growth. Functional polymeric granulate dedicated for osteoconductive, osteoinductive and osteogenesis properties were elaborated. Another approach including the coating for the implant surface with two-phase biocompatible layer including polymeric microspheres and hydrogel carrier, which would provide long-time release of bone and cartilage growth factors around the implant were also done. The polymeric granulate containing βTCP improved bone cells growth, but it some modification has to be done in order to improve structural pores to ensure for better osteoconductivity. The biocompatible coating including PVP hydrogel and polymeric microspheres is still in the development process. © 2020 Elsevier B.V.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85096774372,

title = {The Role of Yersinia enterocolitica O:3 Lipopolysaccharide in Collagen-Induced Arthritis},

author = { K. Kasperkiewicz and A.S. Świerzko and M. Grabowska and J. Szemraj and J. Barski and M. Skurnik and A. Kałużyński and M. Cedzyński},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096774372&doi=10.1155%2f2020%2f7439506&partnerID=40&md5=10467ce39ddaa1b68ce38eff8a5f103c},

doi = {10.1155/2020/7439506},

issn = {23148861},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Immunology Research},

volume = {2020},

publisher = {Hindawi Limited},

abstract = {Yersinia enterocolitica O:3 is mentioned among the most common arthritogenic pathogens. Bacterial components (including lipopolysaccharide (LPS)) may persist in the joint after eradication of infection. Having an adjuvant activity, LPS may enhance production of anticollagen antibodies, involved in the pathogenesis of rheumatoid arthritis. Furthermore, its ability to activate complement contributes to the inflammation. The aim of this work was to investigate whether Yersinia LPS (coinjected with collagen) is associated with arthritis progression or other pathological effects and to elucidate the mechanism of this association. It was demonstrated that murine mannose-binding lectin C (MBL-C) recognizes the inner core heptoses of the Rd1 chemotype LPS of Yersinia. In addition, the Rd1 LPS activates the MBL-associated serine protease 1 (MASP-1) stronger than the S and Ra chemotype LPS and comparable to Klebsiella pneumoniae O:3 LPS. However, in contrast to the latter, Yersinia Rd1 LPS was associated neither with the adjuvancity nor with the enhancement of pathological changes in animal paws/impairment of motility. On the other hand, it seemed to be more hepatotoxic when compared with the other tested endotoxins, while the enlargement of inguinal lymph nodes and drop in hepatic MBL-C expression (at the mRNA level) were independent of LPS chemotype. Our data did not suggest no greater impact Y. enterocolitica O:3 on the development or severity of arthropathy related to anticollagen antibody-induced arthritis in mice, although its interaction with MBL-C and subsequent complement activation may contribute to some adverse effects. © 2020 Katarzyna Kasperkiewicz et al.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85072055835,

title = {Adhesion abilities and biosorption of Cd and Mg by microorganisms - first step for eco-friendly beneficiation of phosphate ore},

author = { H. Rabia and M. Ould Hamou and K. Kasperkiewicz and J. Brożek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072055835&doi=10.1038%2fs41598-019-49406-4&partnerID=40&md5=c2448f69f61e1c4d9fc49a13441d188c},

doi = {10.1038/s41598-019-49406-4},

issn = {20452322},

year  = {2019},

date = {2019-01-01},

journal = {Scientific Reports},

volume = {9},

number = {1},

publisher = {Nature Publishing Group},

abstract = {Chemical reagents used in traditional mineral processing can be toxic and hazardous for the environment. Therefore, the use of biotechnological methods is becoming increasingly important. Great hopes are being placed in the use of microorganisms for bio-beneficiation of raw materials. However, assessment of adhesion abilities of bacteria onto minerals surface as well as biosorption of metals are essential steps before designing final process of each ore beneficiation. The main aim of this work was an investigation of biosorption of Cd and Mg, as well as adhesion abilities of five microorganism species with minerals included in the natural mixture of phosphate ore form Djebel Onk, Algeria. The ore, due to its unique composition, created conditions for adhesion of all five tested microbial strains onto apatite surface during incubation at pH 3. Moreover, Rhodococcus erythropolis CD 130, Pseudomonas fluorescens and Escherichia coli adhered distinctly onto apatite surface during incubation at pH 7. Incubation lasting 20 min at pH 4-6 created the most favorable conditions for biosorption of metals by B. subtilis and adhesion of cells. In case of C. albicans, biosorption of metals as well as adhesion of cells onto the mineral surface were more effective after longer time and in a wider pH range. © 2019, The Author(s).},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85054441792,

title = {The role of complement activating collectins and associated serine proteases in patients with hematological malignancies, receiving high-dose chemotherapy, and autologous hematopoietic stem cell transplantations (Auto-HSCT)},

author = { A.S. Świerzko and M. Michalski and A. Sokolowska and M. Nowicki and L. Eppa and A. Szala-Pozdziej and I. Mitrus and A. Szmigielska-Kaplon and M. Sobczyk-Kruszelnicka and K. Michalak and A. Golos and A. Wierzbowska and S. Giebel and K. Jamroziak and M.L. Kowalski and O. Brzezinska and S. Thiel and J.C. Jensenius and K. Kasperkiewicz and M. Cedzyński},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054441792&doi=10.3389%2ffimmu.2018.02153&partnerID=40&md5=470a8fa1b754325931cb29b6a83bd38f},

doi = {10.3389/fimmu.2018.02153},

issn = {16643224},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Immunology},

volume = {9},

number = {SEP},

publisher = {Frontiers Media S.A.},

abstract = {We conducted a prospective study of 312 patients (194 with multiple myeloma; 118 with lymphomas) receiving high-dose conditioning chemotherapy and autologous hematopoietic stem cell transplantation (auto-HSCT). Polymorphisms of MBL2 and MASP2 genes were investigated and serial measurements of serum concentrations of mannose-binding lectin (MBL), CL-LK collectin and MASP-2 as well as activities of MBL-MASP-1 and MBL-MASP-2 complex were made. Serum samples were taken before conditioning chemotherapy, before HSCT and once weekly after (totally 4-5 samples); in minority of subjects also 1 and/or 3 months post transplantation. The results were compared with data from 267 healthy controls and analyzed in relation to clinical data to explore possible associations with cancer and with chemotherapy-induced medical complications. We found a higher frequency of MBL deficiency-associated genotypes (LXA/O or O/O) among multiple myeloma patients compared with controls. It was however not associated with hospital infections or post-HSCT recovery of leukocytes, but seemed to be associated with the most severe infections during follow-up. Paradoxically, high MBL serum levels were a risk factor for prolonged fever and some infections. The first possible association of MBL2 gene 3'-untranslated region polymorphism with cancer (lymphoma) in Caucasians was noted. Heterozygosity for MASP2 gene +359 A>G mutation was relatively frequent in lymphoma patients who experienced bacteremia during hospital stay. The median concentration of CL-LK was higher in myeloma patients compared with healthy subjects. Chemotherapy induced marked increases in serum MBL and MASP-2 concentrations, prolonged for several weeks and relatively slighter decline in CL-LK level within 1 week. Conflicting findings on the influence of MBL on infections following chemotherapy of myeloma and lymphoma have been reported. Here we found no evidence for an association between MBL deficiency and infection during the short period of neutropenia following conditioning treatment before HSCT. However, we noted a possible protective effect of MBL during follow-up, and suspected that to be fully effective when able to act in combination with phagocytic cells after their recovery. © 2007-2018 Frontiers Media S.A. All Rights Reserved.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85032667179,

title = {Lectin pathway factors in patients suffering from juvenile idiopathic arthritis},

author = { K. Kasperkiewicz and L. Eppa and A.S. Świerzko and M.A. Bartomiejczyk and Z. Zuber and K. Siniewicz-Luzeñczyk and E. Mêyk and M. Matsushita and L. Bak-Romaniszyn and K. Zeman and M. Skurnik and M. Cedzyński},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032667179&doi=10.1038%2ficb.2017.31&partnerID=40&md5=f14eef41d8bd83d0a2f2aad4100cc373},

doi = {10.1038/icb.2017.31},

issn = {08189641},

year  = {2017},

date = {2017-01-01},

journal = {Immunology and Cell Biology},

volume = {95},

number = {8},

pages = {666-675},

publisher = {Nature Publishing Group},

abstract = {Both complement activation and certain infections (including those with Yersinia sp.) may contribute to the pathogenesis of juvenile idiopathic arthritis (JIA). We investigated factors specific for the lectin pathway of complement: Mannose-binding lectin (MBL), ficolins and MBL-associated serine protease-2 (MASP-2), in 144 patients and 98 controls. One hundred and six patients had oligoarticular disease and 38 had polyarticular disease. In 51 patients (out of 133 tested), Yersinia-reactive antibodies were found (JIA Ye + group). MBL deficiency was significantly more frequent in the JIA Ye + group than in patients without Yersinia-reactive antibodies or in controls. Median serum ficolin-2 level was significantly lower (and proportion of values deemed ficolin-2 insufficient greater) in JIA patients irrespective of their Yersinia antibody status. The minority (C) allele at â '64 of the FCN2 gene was less frequent among JIA patients than among control subjects. No differences were found in the frequency of FCN3 gene +1637delC or MASP2 +359 A>G mutations nor for median values of serum ficolin-1, ficolin-3 or MASP-2. However, high levels of serum ficolin-3 were under-represented in patients, in contrast to MBL. MBL, ficolin-1, ficolin-2, ficolin-3 and MASP-2 were also readily detectable in synovial fluid samples but at a considerably lower level than in serum. Our findings suggest a possible role for the lectin pathway in the pathogenesis of JIA, perhaps secondary to a role in host defence, and indicate that investigations on the specificity of lectin pathway recognition molecules towards specific infectious agents in JIA might be fruitful.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84940451045,

title = {Interaction of human mannose-binding lectin (MBL) with Yersinia enterocolitica lipopolysaccharide},

author = { K. Kasperkiewicz and A.S. Świerzko and M.A. Bartlomiejczyk and M. Cedzyński and M. Noszczyńska and K.A. Duda and M. Michalski and M. Skurnik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940451045&doi=10.1016%2fj.ijmm.2015.07.001&partnerID=40&md5=a13275e79aea7f4c6c6f8c15b72162af},

doi = {10.1016/j.ijmm.2015.07.001},

issn = {14384221},

year  = {2015},

date = {2015-01-01},

journal = {International Journal of Medical Microbiology},

volume = {305},

number = {6},

pages = {544-552},

publisher = {Elsevier GmbH},

abstract = {The lipopolysaccharide (LPS) is involved in the interaction between Gram-negative pathogenic bacteria and host. Mannose-binding lectin (MBL), complement-activating soluble pattern-recognition receptor targets microbial glycoconjugates, including LPS. We studied its interactions with a set of Yersinia enterocolitica O:3 LPS mutants. The wild-type strain LPS consists of lipid A (LA) substituted with an inner core oligosaccharide (IC) which in turn is substituted either with the O-specific polysaccharide (OPS) or the outer core hexasaccharide (OC), and sometimes also with the enterobacterial common antigen (ECA). The LPS mutants produced truncated LPS, missing OPS, OC or both, or, in addition, different IC constituents or ECA. MBL bound to LA-IC, LA-IC-OPS and LA-IC-ECA but not LA-IC-OC structures. Moreover, LA-IC substitution with both OPS and ECA prevented the lectin binding. Sequential truncation of the IC heptoses demonstrated that the MBL targets the IC heptose region. Furthermore, microbial growth temperature influenced MBL binding; binding was stronger to bacteria grown at room temperature (22. °C) than to bacteria grown at 37 °C. In conclusion, our results demonstrate that MBL can interact with Y. enterocolitica LPS, however, the in vivo significance of that interaction remains to be elucidated. © 2015 Elsevier GmbH.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84938289994,

title = {Human Microbiome: When a Friend Becomes an Enemy},

author = { M. Muszer and M. Noszczyńska and K. Kasperkiewicz and M. Skurnik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938289994&doi=10.1007%2fs00005-015-0332-3&partnerID=40&md5=db02c868f7d0ad90693c5d80b029750f},

doi = {10.1007/s00005-015-0332-3},

issn = {0004069X},

year  = {2015},

date = {2015-01-01},

journal = {Archivum Immunologiae et Therapiae Experimentalis},

volume = {63},

number = {4},

pages = {287-298},

publisher = {Birkhauser Verlag AG},

abstract = {The microorganisms that inhabit humans are very diverse on different body sites and tracts. Each specific niche contains a unique composition of the microorganisms that are important for a balanced human physiology. Microbial cells outnumber human cells by tenfold and they function as an invisible organ that is called the microbiome. Excessive use of antibiotics and unhealthy diets pose a serious danger to the composition of the microbiome. An imbalance in the microbial community may cause pathological conditions of the digestive system such as obesity, cancer and inflammatory bowel disease; of the skin such as atopic dermatitis, psoriasis and acne and of the cardiovascular system such as atherosclerosis. An unbalanced microbiome has also been associated with neurodevelopmental disorders such as autism and multiple sclerosis. While the microbiome has a strong impact on the development of the host immune system, it is suspected that it can also be the cause of certain autoimmune diseases, including diabetes or rheumatoid arthritis. Despite the enormous progress in the field, the interactions between the human body and its microbiome still remain largely unknown. A better characterization of the interactions may allow for a deeper understanding of human disease states and help to elucidate a possible association between the composition of the microbiome and certain pathologies. This review focuses on general findings that are related to the area and provides no detailed information about the case of study. The aim is to give some initial insight on the studies of the microbiome and its connection with human health. © 2015, The Author(s).},

note = {36},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84942433786,

title = {ECA - Common surface antigen of the bacilli of the Enterobacteriaceae family [ECA - Wspólny antygen powierzchniowy pałeczek rodziny Enterobacteriaceae]},

author = { K. Kasperkiewicz and M. Noszczyńska and A. Piszczek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942433786&partnerID=40&md5=1da607bf0c309be7cb88d62ca64f7da4},

issn = {00794252},

year  = {2015},

date = {2015-01-01},

journal = {Postepy Mikrobiologii},

volume = {54},

number = {2},

pages = {165-174},

publisher = {Polish Society of Microbiologists},

abstract = {Almost all the strains of bacteria belonging to the Enterobacteriaceae family share at least one common antigenic component, ECA, which is not present in other Gram-negative and Gram-positive bacteria. From the observations made with immunofluorescence and immunoferritin techniques, it has been concluded that ECA is localized in the outer leaflet of the outer membrane of Gram-negative enteric bacteria. ECA is a glycolipid consisting of linear trisaccharide repeating units composed of [→3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→]. It occurs in three structural forms: ECAPG linked to phosphatidylglycerol, ECALPS anchored to LPS core region and ECACYC not expressed on the surface. ECA is believed to be connected to the LPS outer core. However, it should be emphasized that Yersinia enterocolitica serotype O:3 mutants defective in outer core synthesis were also ECA-immunogenic. The genes involved in ECA biosynthesis are located in the chromosomal wec gene cluster, from wecA to wecG and the ECA expressions is downregulated at host temperature. So far, ECA has been thoroughly analyzed at the structural and genetic level, however, its significance in vivo has been investigated in relatively few studies. ECA has been linked to pathogenesis in several species of bacteria, although this function seems to differ between the species. ECA has been shown to be involved in the flagellar assembly and motility in Serratia marcescens Also, the ECA-negative mutants of Salmonella enterica serovar Typhimurium proved to be significantly less virulent than the parental strain. ECA as a marker of Enterobacteriaceae family is a valuable indicator of water and food contaminations with enteric bacteria.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84920903555,

title = {Serological characterization of the enterobacterial common antigen substitution of the lipopolysaccharide of Yersinia enterocolitica o:3},

author = { M. Noszczyńska and K. Kasperkiewicz and K.A. Duda and J. Podhorodecka and K. Rabsztyn and K. Gwizdała and A.S. Świerzko and J. Radziejewska-Lebrecht and O. Holst and M. Skurnik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84920903555&doi=10.1099%2fmic.0.083493-0&partnerID=40&md5=e7ef363563f740b94a88bdf3f604463b},

doi = {10.1099/mic.0.083493-0},

issn = {13500872},

year  = {2015},

date = {2015-01-01},

journal = {Microbiology (United Kingdom)},

volume = {161},

number = {1},

pages = {219-227},

publisher = {Society for General Microbiology},

abstract = {Enterobacterial common antigen (ECA) is a polysaccharide present in all members of Enterobacteriaceae anchored either via phosphatidylglycerol (PG) or LPS to the outer leaflet of the outer membrane (ECAPG and ECALPS; respectively). Only the latter form is ECAimmunogenic. We previously demonstrated that Yersinia enterocolitica O: 3 and its rough (Ospecific polysaccharide-negative) mutants were ECA-immunogenic, suggesting that they contained ECALPS; however, it was not known which part of the LPS core region was involved in ECA binding. To address this, we used a set of three deep-rough LPS mutants for rabbit immunization. The polyvalent antisera obtained were: (i) analysed for the presence of anti-LPS and anti-ECA antibodies; (ii) treated with caprylic acid (CA) to precipitate IgM antibodies and protein aggregates; and (iii) adsorbed with live ECA-negative bacteria to obtain specific anti-ECA antisera. We demonstrated the presence of antibodies specific for both ECAPG and ECALPS in all antisera obtained. Both CA treatment and adsorption with ECA-negative bacteria efficiently removed anti-LPS antibodies, resulting in specific anti-ECA sera. The LPS of the ECALPS-positive deepest-rough mutant contained only lipid A and 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) residues of the inner core, suggesting that ECALPS was linked to the Kdo region of LPS in Y. enterocolitica O:3. © 2015 The Authors.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84881240290,

title = {Enterobacterial common antigen and O-specific polysaccharide coexist in the lipopolysaccharide of Yersinia enterocolitica serotype O: 3},

author = { A. Muszyński and K. Rabsztyn and K. Knapska and K.A. Duda and K. Duda-Grychtoł and K. Kasperkiewicz and J. Radziejewska-Lebrecht and O. Holst and M. Skurnik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881240290&doi=10.1099%2fmic.0.066662-0&partnerID=40&md5=2424c3ecc13891b49a6e7ed5d29f8d41},

doi = {10.1099/mic.0.066662-0},

issn = {13500872},

year  = {2013},

date = {2013-01-01},

journal = {Microbiology (United Kingdom)},

volume = {159},

number = {8},

pages = {1782-1793},

abstract = {Yersinia enterocolitica serotype O: 3 produces two types of lipopolysaccharide (LPS) molecules to its surface. In both types the lipid A (LA) structure is substituted by inner core (IC) octasaccharide to which either outer core (OC) hexasaccharide or homopolymeric O-polysaccharide (OPS) is linked. In addition, enterobacterial common antigen (ECA) can be covalently linked to LPS, however, via an unknown linkage. To elucidate the relationship between ECA and LPS in Y. enterocolitica O:3 and the effect of temperature on their expression, LPS was isolated from bacteria grown at 22 °C and 37 °C by consequent hot phenol/water and phenol-chloroform-light petroleum extractions to obtain LPS preparations free of ECA linked to glycerophospholipid. In immunoblotting, monoclonal antibodies TomA6 and 898, specific for OPS and ECA, respectively, reacted both with ladder-like bands and with a slower-migrating smear suggesting that the ECA and OPS epitopes coexist on the same molecules. These results were supported by immunoblotting with a monovalent Y. enterocolitica O:3 ECAspecific rabbit antiserum. Also, two or three 898-positive (and monovalent-positive) TomA6-negative bands migrated at the level of the LA-IC band in LPS samples from certain OC mutants, most likely representing LA-IC molecules carrying 1-3 ECA repeat units but no OPS. These bands were also present in Y. enterocolitica O: 9 OC mutants; however, coexistence of ECA and OPS in the same molecules could not be detected. Finally, the LA-IC-ECA bands were missing from LPS of bacteria grown at 37 °C and also the general reduction in wild-type bacteria of ECA-specific monovalentreactive material at 37 °C suggested that temperature regulates the expression of ECA. Indeed, RNAsequencing analysis showed significant downregulation of the ECA biosynthetic gene cluster at 37 °C. © 2013 SGM.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84863681678,

title = {Bacterial cell surface structures in Yersinia enterocolitica},

author = { N. Białas and K. Kasperkiewicz and J. Radziejewska-Lebrecht and M. Skurnik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84863681678&doi=10.1007%2fs00005-012-0168-z&partnerID=40&md5=bab1bed8a6f63d45318717ab2ffd2f89},

doi = {10.1007/s00005-012-0168-z},

issn = {0004069X},

year  = {2012},

date = {2012-01-01},

journal = {Archivum Immunologiae et Therapiae Experimentalis},

volume = {60},

number = {3},

pages = {199-209},

abstract = {Yersinia enterocolitica is a widespread member of the family of Enterobacteriaceae that contains both nonvirulent and virulent isolates. Pathogenic Y. enterocolitica strains, especially belonging to serotypes O:3, O:5,27, O:8 and O:9 are etiologic agents of yersiniosis in animals and humans. Y. enterocolitica cell surface structures that play a significant role in virulence have been subject to many investigations. These include outer membrane (OM) glycolipids such as lipopolysaccharide (LPS) and enterobacterial common antigen (ECA) and several cell surface adhesion proteins present only in virulent Y. enterocolitica, i.e., Inv, YadA and Ail. While the yadA gene is located on the Yersinia virulence plasmid the Ail, Inv, LPS and ECA are chromosomally encoded. These structures ensure the correct architecture of the OM, provide adhesive properties as well as resistance to antimicrobial peptides and to host innate immune response mechanisms. © L. Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland 2012.},

note = {29},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79960876701,

title = {Characterization of anti-ECA antibodies in rabbit antiserum against rough Yersinia enterocolitica O:3},

author = { K. Rabsztyn and K. Kasperkiewicz and K.A. Duda and C.M. Li and M. Łukasik and J. Radziejewska-Lebrecht and M. Skurnik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960876701&doi=10.1134%2fS0006297911070145&partnerID=40&md5=008804075e9da057a06fd9cdfc890550},

doi = {10.1134/S0006297911070145},

issn = {00062979},

year  = {2011},

date = {2011-01-01},

journal = {Biochemistry (Moscow)},

volume = {76},

number = {7},

pages = {832-839},

publisher = {Maik Nauka Publishing / Springer SBM},

abstract = {Enterobacterial common antigen (ECA) is a characteristic surface component in bacteria belonging to the Enterobacteriaceae family. It is generally integrated in the outer membrane via a linkage to phosphatidylglycerol (ECA PG) and at the same time in some special cases via a linkage to lipopolysaccharide (ECALPS); the latter form is immunogenic. Yersinia enterocolitica O:3 expresses both ECAPG and ECALPS. To study whether ECA-immunogenicity of Y. enterocolitica O:3 is temperature-regulated, rabbits were immunized with ECA-expressing Y. enterocolitica O:3 bacteria grown at 22 and 37°C. To induce minimal amount of anti-LPS antibodies, immunization was performed with YeO3-c-trs8-R, an LPS mutant missing both O-polysaccharide and the outer core hexasaccharide. However, abundant antibodies specific for LPS core were still present in the obtained antisera such that the reactivity of ECA-specific antibodies could not be detected. To obtain "monovalent" anti-ECA antisera, the sera were absorbed with ECA-negative bacteria. Absorption with live bacteria removed efficiently the anti-LPS antibodies, whereas this was not the case with boiled bacteria. Western blotting revealed that the specificity of the monovalent anti-ECA antiserum was different from that of a monoclonal anti-ECA antibody (mAb 898) as it did not react with ECAPG, and this suggested that in Y. enterocolitica O:3 ECALPS only one or two ECA repeat unit(s) is/are linked to LPS. Both ECAPG and ECALPS expression were found to be regulated by temperature and repressed at 37°C. © 2011 Pleiades Publishing, Ltd.},

note = {13},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-70450187189,

title = {Identification and role of a 6-deoxy-4-keto-hexosamine in the lipopolysaccharide outer core of yersinia enterocolitica serotype O:3},

author = { E. Pinta and K.A. Duda and A. Hanuszkiewicz and Z. Kaczyński and B. Lindner and W.L. Miller and H. Hyytiäinen and C. Vogel and S. Borowski and K. Kasperkiewicz and J.S. Lam and J. Radziejewska-Lebrecht and M. Skurnik and O. Holst},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-70450187189&doi=10.1002%2fchem.200901255&partnerID=40&md5=d3b242e9eeeb8c35973e5d1e2d12fd27},

doi = {10.1002/chem.200901255},

issn = {09476539},

year  = {2009},

date = {2009-01-01},

journal = {Chemistry - A European Journal},

volume = {15},

number = {38},

pages = {9747-9754},

abstract = {The outer core (OC) region of Yersinia enterocolitica serotype 0:3 lipopolysaccharide is a hexasaccharide essential for the integrity of the outer membrane. It is involved in resistance against cationic antimicrobial peptides and plays a role in virulence during early phases of infection. We show here that the proximal residue of the OC hexasaccharide is a rarely encountered 4-keto-hexosamine, 2-acetamido2,6-dideoxy-D-xylo-hex-4-ulopyranose (Sugp) and that WbcP is a UDPGlcNAc-4,6-dehydratase enzyme responsible for the biosynthesis of the nucleotide-activated form of this rare sugar converting UDP-2-acetamido-2deoxy-D-glucopyranose (UDP-D-GlcpNAc) to UDP-2-acetamido-2,6- dideoxy-D-xylo-hex-4-ulopyranose (UDPSugp). In an aqueous environment, the 4-keto group of this sugar was present in the 4-dihydroxy form, due to hydration. Furthermore, evidence is provided that the axial 4-hydroxy group of this dihydroxy function was crucial for the biological role of the OC, that is, in the bacteriophage and enterocoliticin receptor structure and in the epitope of a monoclonal antibody. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, biosynthesis.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-64649091101,

title = {ECA-immunogenicity of Proteus mirabilis strains},

author = { K.A. Duda and K.T. Duda and A. Beczała and K. Kasperkiewicz and J. Radziejewska-Lebrecht and M. Skurnik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-64649091101&doi=10.1007%2fs00005-009-0018-9&partnerID=40&md5=10d4f74bf467437bc14d60290eeb04f5},

doi = {10.1007/s00005-009-0018-9},

issn = {0004069X},

year  = {2009},

date = {2009-01-01},

journal = {Archivum Immunologiae et Therapiae Experimentalis},

volume = {57},

number = {2},

pages = {147-151},

abstract = {Introduction: Bacteria of the genus Proteus are opportunistic pathogens and cause mainly urinary tract infections. They also play a role in the pathogenesis of reactive arthritis (RA). Patients suffering from Yersinia-triggered RA often carry high titers of antibodies specific to enterobacterial common antigen (ECA). The immunogenicity of ECA has not received much attention thus far and studies have focused mainly on the ECA of Escherichia coli and Yersinia enterocolitica. In this paper the ECA-immunogenicity of Proteus mirabilis is elucidated using two wild-type strains (S1959 and O28) as well as their rough (R) derivative strains R110/1959, which expresses lipopolysaccharide (LPS) with a full core, and R4/O28, which expresses LPS with only an inner core. Materials and Methods: Rabbit polyclonal antisera were produced by immunization with boiled suspensions of the four P. mirabilis strains. The antisera were tested for the presence of antibodies specific to ECA by Western blotting using glycerophospholipid- linked ECA (ECA PG ) of Salmonella montevideo as antigen. Lipopolysaccharide (LPS) was isolated from the four strains by the hot phenol/water procedure in which ECA PG is co-extracted with LPS and by the phenol/chloroform/ petroleum ether extraction that results in the isolation of LPS and/or LPS-linked ECA (ECA LPS ) free of ECA PG . The LPS preparations were tested for the presence of ECA by Western blotting using ECA-specific antibodies. Results: The results demonstrated that all four P. mirabilis strains were ECA immunogenic. The rabbit antisera immunized by the four strains all contained ECA-specific antibodies. Analysis of the LPS preparations demonstrated that the P. mirabilis wild-type strains O28 and S1959 and the Ra mutant strain R110/1959 expressed ECA LPS , suggesting that it induced the anti-ECA antibody responses. Only the presence of ECA PG could be demonstrated in the Rc mutant strain R4/O28. Conclusions: These results therefore suggest that, similar to E. coli, LPS with a full core is also required as the acceptor of ECA for P. mirabilis strains to produce ECA LPS . Since ECA PG is not immunogenic unless combined with some proteins, it is likely that ECA PG -protein complexes formed during the intravenous immunization with the Rc mutant strain R4/O28. © 2009 L. Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland.},

note = {13},

keywords = {},

pubstate = {published},

tppubtype = {article}

}