• mgr Jerzy Karczewski
Stanowisko: St.specj.b-techn.
Jednostka: Wydział Nauk Przyrodniczych
Adres: 40-032 Katowice, ul. Jagiellońska 28
Piętro: III
Numer pokoju: C-353
Telefon: (32) 2009 371
E-mail: jerzy.karczewski@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 57196774672
Publikacje z bazy Scopus
2023
Woźnica, A.; Karczewski, J.; Gwiazda, R.; Karcz, J.; Bernaś, T.; Libera, M.; Babczyńska, A.; Łozowski, B.
Combined complementary imaging techniques in morphological analysis of Spongilla lacustris (Linnaeus 1759) Journal Article
In: Limnology and Oceanography: Methods, vol. 21, no. 3, pp. 141-151, 2023, ISSN: 15415856.
@article{2-s2.0-85147301610,
title = {Combined complementary imaging techniques in morphological analysis of Spongilla lacustris (Linnaeus 1759)},
author = { A. Woźnica and J. Karczewski and R. Gwiazda and J. Karcz and T. Bernaś and M. Libera and A. Babczyńska and B. Łozowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85147301610&doi=10.1002%2flom3.10534&partnerID=40&md5=11eb042255adfefe285c387664cfd151},
doi = {10.1002/lom3.10534},
issn = {15415856},
year = {2023},
date = {2023-01-01},
journal = {Limnology and Oceanography: Methods},
volume = {21},
number = {3},
pages = {141-151},
publisher = {John Wiley and Sons Inc},
abstract = {Sponges, like Spongilla lacustris, as filter feeders, play an essential role in water purification in aquatic ecosystems. The body of this demosponge, in general, consists of both organic soft skeleton and a siliceous scaffold. Their construction of the spicule-bundling scaffold as mechanical support for skeletal organic mesohyl seems crucial for filtration efficiency. Understanding the structure of the sponge's biosilica-based scaffold as well as its location within three-dimensional (3D) skeletal construct requires the introduction of effective analytical methods. The investigations focused on the morphology and architecture of skeletal elements of S. lacustris utilizing the combination of X-ray computed microtomography (μCT) and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM/EDS). The construction details, surface morphology and chemical composition of the sponge scaffold are presented. μCT provided the reconstructed 3D images of skeleton structures, including longitudinally and transversely oriented bundles of overlapping spicules, forming a ladder-like construction as well as the length, geometric distribution, and the surface of the spicules. Further analyses based on SEM/EDS confirmed the proper identification of the structures and their localization and revealed a high abundance of silicon and a low amount of carbon and oxygen in spicules, high abundance of silicon, carbon and oxygen in layered membranes surrounding the bundles of spicules but predominating carbon in the pinacoderm. Combination of these techniques provides a unique image of the sponge body morphology. Verified set of tools may be used for further analyses of sponge body mechanics. © 2023 Association for the Sciences of Limnology and Oceanography.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Sponges, like Spongilla lacustris, as filter feeders, play an essential role in water purification in aquatic ecosystems. The body of this demosponge, in general, consists of both organic soft skeleton and a siliceous scaffold. Their construction of the spicule-bundling scaffold as mechanical support for skeletal organic mesohyl seems crucial for filtration efficiency. Understanding the structure of the sponge's biosilica-based scaffold as well as its location within three-dimensional (3D) skeletal construct requires the introduction of effective analytical methods. The investigations focused on the morphology and architecture of skeletal elements of S. lacustris utilizing the combination of X-ray computed microtomography (μCT) and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM/EDS). The construction details, surface morphology and chemical composition of the sponge scaffold are presented. μCT provided the reconstructed 3D images of skeleton structures, including longitudinally and transversely oriented bundles of overlapping spicules, forming a ladder-like construction as well as the length, geometric distribution, and the surface of the spicules. Further analyses based on SEM/EDS confirmed the proper identification of the structures and their localization and revealed a high abundance of silicon and a low amount of carbon and oxygen in spicules, high abundance of silicon, carbon and oxygen in layered membranes surrounding the bundles of spicules but predominating carbon in the pinacoderm. Combination of these techniques provides a unique image of the sponge body morphology. Verified set of tools may be used for further analyses of sponge body mechanics. © 2023 Association for the Sciences of Limnology and Oceanography.
2022
Woźnica, A.; Karczewski, J.; Kliś, C.; Długosz, J.; Ziemski, P.; Nowak, A.; Bernaś, T.
Modeling of Nitrification Kinetics in a Respirometric Biosensor under Suboptimal Conditions Journal Article
In: Water (Switzerland), vol. 14, no. 13, 2022, ISSN: 20734441.
@article{2-s2.0-85133266871,
title = {Modeling of Nitrification Kinetics in a Respirometric Biosensor under Suboptimal Conditions},
author = { A. Woźnica and J. Karczewski and C. Kliś and J. Długosz and P. Ziemski and A. Nowak and T. Bernaś},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85133266871&doi=10.3390%2fw14132031&partnerID=40&md5=1e4f5a74cdc187870d4cd664f6a7e521},
doi = {10.3390/w14132031},
issn = {20734441},
year = {2022},
date = {2022-01-01},
journal = {Water (Switzerland)},
volume = {14},
number = {13},
publisher = {MDPI},
abstract = {Sensitive detection with cell biosensors requires optimization of their working conditions and standardization of the response in variable physicochemical conditions. The introduction of an analyte to a sensor, which contributes to this variability, may account for the modeling of microbial metabolism. We constructed a multiparameter model of a water toxicity sensor of Automatic Biodetector for Water Toxicity (ABTOW), developed by our group and based on nitrifying bacteria. The model describes the kinetics of nitrification as a function of four orthogonal parameters: temperature, pH, oxygen and ammonium concentration. Furthermore, we characterized the signal-to-noise ratio (SNR) of the ABTOW readout as a function of these parameters. Thus, a region of parameter space corresponding to optimal ABTOW operation is identified and its sensitivity quantified. We applied the model to describe the ABTOW performance in non-equilibrium conditions produced by rapid changes in pH and temperature. In sum, the model based on four physicochemical parameters describes changes in the biosensor's activity, the biological element of which are nitrifying bacteria characterized by simple chemolithoautotrophic metabolism. The description of reaction kinetics through multiparameter modeling in combination with stability analysis can find application in process control in biotechnology, biodetection and environmental research. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Sensitive detection with cell biosensors requires optimization of their working conditions and standardization of the response in variable physicochemical conditions. The introduction of an analyte to a sensor, which contributes to this variability, may account for the modeling of microbial metabolism. We constructed a multiparameter model of a water toxicity sensor of Automatic Biodetector for Water Toxicity (ABTOW), developed by our group and based on nitrifying bacteria. The model describes the kinetics of nitrification as a function of four orthogonal parameters: temperature, pH, oxygen and ammonium concentration. Furthermore, we characterized the signal-to-noise ratio (SNR) of the ABTOW readout as a function of these parameters. Thus, a region of parameter space corresponding to optimal ABTOW operation is identified and its sensitivity quantified. We applied the model to describe the ABTOW performance in non-equilibrium conditions produced by rapid changes in pH and temperature. In sum, the model based on four physicochemical parameters describes changes in the biosensor's activity, the biological element of which are nitrifying bacteria characterized by simple chemolithoautotrophic metabolism. The description of reaction kinetics through multiparameter modeling in combination with stability analysis can find application in process control in biotechnology, biodetection and environmental research. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Woźnica, A.; Karczewski, J.; Bernaś, T.; Świątek, P.; Drab, M.; Surma, R.; Krzyżowski, M.; Łozowski, B.; Gwiazda, R.; Libera, M.; Absalon, D.; Babczyńska, A.
In: European Zoological Journal, vol. 89, no. 1, pp. 1002-1017, 2022, ISSN: 24750263, (1).
@article{2-s2.0-85135441745,
title = {The spatial structure (3D) and mechanical properties of the sponge Spongilla lacustris L. (Porifera: Spongillida) skeleton as a potential tensegral architecture},
author = { A. Woźnica and J. Karczewski and T. Bernaś and P. Świątek and M. Drab and R. Surma and M. Krzyżowski and B. Łozowski and R. Gwiazda and M. Libera and D. Absalon and A. Babczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135441745&doi=10.1080%2f24750263.2022.2105964&partnerID=40&md5=d32c8660b692cf631fb930f88c6b6495},
doi = {10.1080/24750263.2022.2105964},
issn = {24750263},
year = {2022},
date = {2022-01-01},
journal = {European Zoological Journal},
volume = {89},
number = {1},
pages = {1002-1017},
publisher = {Taylor and Francis Ltd.},
abstract = {Complex biological systems often provide ready solutions for contemporary engineering. One such organism might be sponges, primitive, tissueless animals whose evolution over 600 million years has allowed them to become highly specialized. An example of such an organism is the freshwater sponge Spongilla lacustris L., an organism that filters water. This study aimed to investigate the 3D structure of the aforementioned sponge using a broad spectrum of techniques such as Microcomputed Tomography (µCT), Scanning Electron Microscopy (SEM), Confocal Laser Scanning Microscopy (CLSM), and Light Microscopy. Additionally, these techniques have been used to correlate sponge architecture with mechanical properties using the concept of tensegrity, i.e., the feature of architectural structures that self-stabilize by balancing multidirectional, often opposing, tensile and compressive forces. A more detailed look at the structure of the sponge skeleton reveals that it is based on two elements: rigid siliceous spicules, chitin in in fibres with cementing collagen-type spongin material. The coexistence of these elements in the sponge structure determines the mechanical properties and, consequently, the sponge skeleton’s postulated tensegrity. Our observations indicate that the integrity of loose megascleres is realized by sponging material surrounding the bundles of spicules. Our distinction of skeletal elements was determined by the number of spicules in the bundle, the direction of spicule position relative to the main body axis, and the way the elements were connected. The arrangement of the bundles described above has important implications for the mechanical properties of the sponge skeleton and, consequently, for the tensegrity hypothesis. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Complex biological systems often provide ready solutions for contemporary engineering. One such organism might be sponges, primitive, tissueless animals whose evolution over 600 million years has allowed them to become highly specialized. An example of such an organism is the freshwater sponge Spongilla lacustris L., an organism that filters water. This study aimed to investigate the 3D structure of the aforementioned sponge using a broad spectrum of techniques such as Microcomputed Tomography (µCT), Scanning Electron Microscopy (SEM), Confocal Laser Scanning Microscopy (CLSM), and Light Microscopy. Additionally, these techniques have been used to correlate sponge architecture with mechanical properties using the concept of tensegrity, i.e., the feature of architectural structures that self-stabilize by balancing multidirectional, often opposing, tensile and compressive forces. A more detailed look at the structure of the sponge skeleton reveals that it is based on two elements: rigid siliceous spicules, chitin in in fibres with cementing collagen-type spongin material. The coexistence of these elements in the sponge structure determines the mechanical properties and, consequently, the sponge skeleton’s postulated tensegrity. Our observations indicate that the integrity of loose megascleres is realized by sponging material surrounding the bundles of spicules. Our distinction of skeletal elements was determined by the number of spicules in the bundle, the direction of spicule position relative to the main body axis, and the way the elements were connected. The arrangement of the bundles described above has important implications for the mechanical properties of the sponge skeleton and, consequently, for the tensegrity hypothesis. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
2021
Dzionek, A.; Wojcieszyńska, D.; Adamczyk-Habrajska, M.; Karczewski, J.; Potocka, I. W.; Guzik, U.
Xanthan gum as a carrier for bacterial cell entrapment: Developing a novel immobilised biocatalyst Journal Article
In: Materials Science and Engineering C, vol. 118, 2021, ISSN: 09284931, (6).
@article{2-s2.0-85090585955,
title = {Xanthan gum as a carrier for bacterial cell entrapment: Developing a novel immobilised biocatalyst},
author = { A. Dzionek and D. Wojcieszyńska and M. Adamczyk-Habrajska and J. Karczewski and I.W. Potocka and U. Guzik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090585955&doi=10.1016%2fj.msec.2020.111474&partnerID=40&md5=4a58bcdb7fc091b5c8bf180f466e858d},
doi = {10.1016/j.msec.2020.111474},
issn = {09284931},
year = {2021},
date = {2021-01-01},
journal = {Materials Science and Engineering C},
volume = {118},
publisher = {Elsevier Ltd},
abstract = {Xanthan gum (XAN) is a widely used polysaccharide in various industries. Because of its unique properties, in this study, an attempt was made to adopt the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen. The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%. A negative impact of entrapment was noted for strain Planococcus sp. S5, which showed a survival rate in the 93–51% range. To achieve good mechanical properties of the composites, they were additionally hardened using polydopamine (PDA). XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules. Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98–99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive. This study also presents the results of naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora. Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications. © 2020 Elsevier B.V.},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Xanthan gum (XAN) is a widely used polysaccharide in various industries. Because of its unique properties, in this study, an attempt was made to adopt the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen. The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%. A negative impact of entrapment was noted for strain Planococcus sp. S5, which showed a survival rate in the 93–51% range. To achieve good mechanical properties of the composites, they were additionally hardened using polydopamine (PDA). XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules. Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98–99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive. This study also presents the results of naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora. Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications. © 2020 Elsevier B.V.
2018
Tulik, M.; Karczewski, J.; Szeliga, N.; Jura-Morawiec, J.; Jarzyna, I.
Morphological characteristics and allometric relationships of shoot in two undergrowth plants: Polygonatum odoratum and Polygonatum multiflorum Journal Article
In: Forests, vol. 9, no. 12, 2018, ISSN: 19994907, (3).
@article{2-s2.0-85058999663,
title = {Morphological characteristics and allometric relationships of shoot in two undergrowth plants: Polygonatum odoratum and Polygonatum multiflorum},
author = { M. Tulik and J. Karczewski and N. Szeliga and J. Jura-Morawiec and I. Jarzyna},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058999663&doi=10.3390%2ff9120783&partnerID=40&md5=9b6581b682355c7adb0b3ca2f339b1ec},
doi = {10.3390/f9120783},
issn = {19994907},
year = {2018},
date = {2018-01-01},
journal = {Forests},
volume = {9},
number = {12},
publisher = {MDPI AG},
abstract = {The main purpose of this investigation was to describe the spatial arrangement of shoot tissues, as seen in transverse section, and allometric relationships in two contrasting species of Polygonatum i.e., Polygonatum odoratum which commonly grows in mixed pine-oak forest with shoots rectangular in shape, and Polygonatum multiflorum found in oak-hornbeam forest with cylindrical shoots. The mass and length of the aerial shoots of each individual plant were measured. The shoot regions of each plant were then categorized as basal (b), central (c) or apical (a). Transverse sections of these shoot regions were subsequently cut, and the following parameters were measured: (1) Diameter of shoots, (2) thickness of the outer and inner zones of parenchyma and (3) thickness of the sclerenchyma zone. Additional allometric relationships between the various measurements were computed and determined as Pearson's correlation coefficients (r). Both species of Polygonatum differed significantly with respect to the length, diameter and thickness of the outer zone of parenchyma. Shoots of P. multiflorum were taller but narrower than those of P. odoratum, which had a significantly wider zone of outer parenchyma. Allometric relationships were stronger for P. multiflorum, and for both species, they were generally stronger in the basal part of the shoot. We conclude that in P. multiflorum, the strong correlation between the diameter and length of the shoot seems to be important to the growth in shaded environments. © 2018 by the authors.},
note = {3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The main purpose of this investigation was to describe the spatial arrangement of shoot tissues, as seen in transverse section, and allometric relationships in two contrasting species of Polygonatum i.e., Polygonatum odoratum which commonly grows in mixed pine-oak forest with shoots rectangular in shape, and Polygonatum multiflorum found in oak-hornbeam forest with cylindrical shoots. The mass and length of the aerial shoots of each individual plant were measured. The shoot regions of each plant were then categorized as basal (b), central (c) or apical (a). Transverse sections of these shoot regions were subsequently cut, and the following parameters were measured: (1) Diameter of shoots, (2) thickness of the outer and inner zones of parenchyma and (3) thickness of the sclerenchyma zone. Additional allometric relationships between the various measurements were computed and determined as Pearson's correlation coefficients (r). Both species of Polygonatum differed significantly with respect to the length, diameter and thickness of the outer zone of parenchyma. Shoots of P. multiflorum were taller but narrower than those of P. odoratum, which had a significantly wider zone of outer parenchyma. Allometric relationships were stronger for P. multiflorum, and for both species, they were generally stronger in the basal part of the shoot. We conclude that in P. multiflorum, the strong correlation between the diameter and length of the shoot seems to be important to the growth in shaded environments. © 2018 by the authors.
2016
Stanimirova, I.; Woźnica, A.; Płociniczak, T.; Kwaśniewski, M.; Karczewski, J.
In: Talanta, vol. 160, pp. 148-156, 2016, ISSN: 00399140, (1).
@article{2-s2.0-84978481147,
title = {A modified weighted mixture model for the interpretation of spatial and temporal changes in the microbial communities in drinking water reservoirs using compositional phospholipid fatty acid data},
author = { I. Stanimirova and A. Woźnica and T. Płociniczak and M. Kwaśniewski and J. Karczewski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978481147&doi=10.1016%2fj.talanta.2016.07.006&partnerID=40&md5=e0f69a1e3ca875379b031e8d2d369b65},
doi = {10.1016/j.talanta.2016.07.006},
issn = {00399140},
year = {2016},
date = {2016-01-01},
journal = {Talanta},
volume = {160},
pages = {148-156},
publisher = {Elsevier B.V.},
abstract = {The aim of this work was to check whether a methodology based on the analysis of data that contain the entire phospholipid fatty acid, PLFA, compositions of water samples can be successfully used to interpret spatial and temporal changes in the microbial communities in water reservoirs. The proposed methodology consists of the construction of a modified weighted multivariate mixture model for the PLFA profiles of the water samples collected in a given monitoring campaign and the identification of latent PLFA components through a comparison with the known PLFA profiles of some cultivated or non-cultivated microbial communities. A 16S rDNA analysis of some of the selected water samples in the monitoring campaign was performed in order to verify the results of the PLFA analysis. The results showed that the proposed methodology can be useful for a dynamic and sensitive evaluation of changes in the microbial quality of water before and after flash flooding and can help in taking a decision regarding further risk assessment. © 2016 Elsevier B.V.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of this work was to check whether a methodology based on the analysis of data that contain the entire phospholipid fatty acid, PLFA, compositions of water samples can be successfully used to interpret spatial and temporal changes in the microbial communities in water reservoirs. The proposed methodology consists of the construction of a modified weighted multivariate mixture model for the PLFA profiles of the water samples collected in a given monitoring campaign and the identification of latent PLFA components through a comparison with the known PLFA profiles of some cultivated or non-cultivated microbial communities. A 16S rDNA analysis of some of the selected water samples in the monitoring campaign was performed in order to verify the results of the PLFA analysis. The results showed that the proposed methodology can be useful for a dynamic and sensitive evaluation of changes in the microbial quality of water before and after flash flooding and can help in taking a decision regarding further risk assessment. © 2016 Elsevier B.V.
2015
Karcz, J.; Woźnica, A.; Binkowski, M.; Klonowska-Olejnik, M.; Bernaś, T.; Karczewski, J.; Migula, P.
In: Folia Histochemica et Cytobiologica, vol. 53, no. 1, pp. 88-95, 2015, ISSN: 02398508, (2).
@article{2-s2.0-84927722789,
title = {SEM-EDS and X-ray micro computed tomography studies of skeletal surface pattern and body structure in the freshwater sponge Spongilla lacustris collected from Goczalkowice reservoir habit (Southern Poland)},
author = { J. Karcz and A. Woźnica and M. Binkowski and M. Klonowska-Olejnik and T. Bernaś and J. Karczewski and P. Migula},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84927722789&doi=10.5603%2fFHC.a2015.0002&partnerID=40&md5=bbf1fb0ce9671ccffdc382e47d994831},
doi = {10.5603/FHC.a2015.0002},
issn = {02398508},
year = {2015},
date = {2015-01-01},
journal = {Folia Histochemica et Cytobiologica},
volume = {53},
number = {1},
pages = {88-95},
publisher = {Via Medica},
abstract = {Introduction. Freshwater sponges are common animals of most aquatic ecosystems. They feed by filtering small particles from the water, and so are thought to be sensitive indicators of pollution. Sponges are strongly associated with the abiotic environment and are therefore used as bioindicators for monitoring of water quality in water habitats. Among the freshwater sponges, Spongilla lacustris is one of the classic models used to study evolution, gene regulation, development, physiology and structural biology in animal water systems. It is also important in diagnostic of aquatic environments. The aim of this study was to characterize and visualize three-dimensional architecture of sponge body and measure skeleton elements of S. lacustris from Goczalkowice reservoir for identification purposes. Material and methods. The scanning electron microscopy with an energy dispersive X-ray microanalysis (SEM- -EDS) and X-ray micro computed tomography (micro-CT) were used to provide non-invasive visualization of the three-dimensional architecture of Spongilla lacustris body. Results. We showed that sponge skeleton was not homogeneous in composition and comprised several forms of skeleton organization. Ectosomal skeleton occurred as spicular brushes at apices of primary fibres with cementing spongin material. Choanosomal skeletal architecture was alveolate with pauci- to multispicular primary fibres connected by paucispicular transverse fibres, made by megascleres embedded in a scanty spongin matrix both in the choanosome and at the sponge surface. In contrast, microscleres were irregularly scattered in choanosome and skeletal surface. Furthermore, SEM-EDS studies showed that the distribution of silica in megascleres and microscleres was observed along the spicules and sponge surface areas. Conclusions. In conclusion, we showed that the combination of SEM-EDS and micro-CT microscopy techniques allowed obtaining a complete picture of the sponge spatial architecture. © Polish Society for Histochemistry and Cytochemistry.},
note = {2},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Introduction. Freshwater sponges are common animals of most aquatic ecosystems. They feed by filtering small particles from the water, and so are thought to be sensitive indicators of pollution. Sponges are strongly associated with the abiotic environment and are therefore used as bioindicators for monitoring of water quality in water habitats. Among the freshwater sponges, Spongilla lacustris is one of the classic models used to study evolution, gene regulation, development, physiology and structural biology in animal water systems. It is also important in diagnostic of aquatic environments. The aim of this study was to characterize and visualize three-dimensional architecture of sponge body and measure skeleton elements of S. lacustris from Goczalkowice reservoir for identification purposes. Material and methods. The scanning electron microscopy with an energy dispersive X-ray microanalysis (SEM- -EDS) and X-ray micro computed tomography (micro-CT) were used to provide non-invasive visualization of the three-dimensional architecture of Spongilla lacustris body. Results. We showed that sponge skeleton was not homogeneous in composition and comprised several forms of skeleton organization. Ectosomal skeleton occurred as spicular brushes at apices of primary fibres with cementing spongin material. Choanosomal skeletal architecture was alveolate with pauci- to multispicular primary fibres connected by paucispicular transverse fibres, made by megascleres embedded in a scanty spongin matrix both in the choanosome and at the sponge surface. In contrast, microscleres were irregularly scattered in choanosome and skeletal surface. Furthermore, SEM-EDS studies showed that the distribution of silica in megascleres and microscleres was observed along the spicules and sponge surface areas. Conclusions. In conclusion, we showed that the combination of SEM-EDS and micro-CT microscopy techniques allowed obtaining a complete picture of the sponge spatial architecture. © Polish Society for Histochemistry and Cytochemistry.
2013
Koprowski, R.; Wróbel, Z.; Kleszcz, A.; Wilczyński, S.; Woźnica, A.; Łozowski, B.; Pilarczyk, M.; Karczewski, J.; Migula, P.
Mobile sailing robot for automatic estimation of fish density and monitoring water quality Journal Article
In: BioMedical Engineering Online, vol. 12, no. 1, 2013, ISSN: 1475925X, (9).
@article{2-s2.0-84880418821,
title = {Mobile sailing robot for automatic estimation of fish density and monitoring water quality},
author = { R. Koprowski and Z. Wróbel and A. Kleszcz and S. Wilczyński and A. Woźnica and B. Łozowski and M. Pilarczyk and J. Karczewski and P. Migula},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84880418821&doi=10.1186%2f1475-925X-12-60&partnerID=40&md5=7f25d9525b41d51dea739062a7e69c2c},
doi = {10.1186/1475-925X-12-60},
issn = {1475925X},
year = {2013},
date = {2013-01-01},
journal = {BioMedical Engineering Online},
volume = {12},
number = {1},
publisher = {BioMed Central Ltd.},
abstract = {Introduction: The paper presents the methodology and the algorithm developed to analyze sonar images focused on fish detection in small water bodies and measurement of their parameters: volume, depth and the GPS location. The final results are stored in a table and can be exported to any numerical environment for further analysis.Material and method: The measurement method for estimating the number of fish using the automatic robot is based on a sequential calculation of the number of occurrences of fish on the set trajectory. The data analysis from the sonar concerned automatic recognition of fish using the methods of image analysis and processing.Results: Image analysis algorithm, a mobile robot together with its control in the 2.4 GHz band and full cryptographic communication with the data archiving station was developed as part of this study. For the three model fish ponds where verification of fish catches was carried out (548; 171 and 226 individuals), the measurement error for the described method was not exceeded 8%.Summary: Created robot together with the developed software has features for remote work also in the variety of harsh weather and environmental conditions, is fully automated and can be remotely controlled using Internet. Designed system enables fish spatial location (GPS coordinates and the depth). The purpose of the robot is a non-invasive measurement of the number of fish in water reservoirs and a measurement of the quality of drinking water consumed by humans, especially in situations where local sources of pollution could have a significant impact on the quality of water collected for water treatment for people and when getting to these places is difficult. The systematically used robot equipped with the appropriate sensors, can be part of early warning system against the pollution of water used by humans (drinking water; natural swimming pools) which can be dangerous for their health. © 2013 Koprowski et al.; licensee BioMed Central Ltd.},
note = {9},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Introduction: The paper presents the methodology and the algorithm developed to analyze sonar images focused on fish detection in small water bodies and measurement of their parameters: volume, depth and the GPS location. The final results are stored in a table and can be exported to any numerical environment for further analysis.Material and method: The measurement method for estimating the number of fish using the automatic robot is based on a sequential calculation of the number of occurrences of fish on the set trajectory. The data analysis from the sonar concerned automatic recognition of fish using the methods of image analysis and processing.Results: Image analysis algorithm, a mobile robot together with its control in the 2.4 GHz band and full cryptographic communication with the data archiving station was developed as part of this study. For the three model fish ponds where verification of fish catches was carried out (548; 171 and 226 individuals), the measurement error for the described method was not exceeded 8%.Summary: Created robot together with the developed software has features for remote work also in the variety of harsh weather and environmental conditions, is fully automated and can be remotely controlled using Internet. Designed system enables fish spatial location (GPS coordinates and the depth). The purpose of the robot is a non-invasive measurement of the number of fish in water reservoirs and a measurement of the quality of drinking water consumed by humans, especially in situations where local sources of pollution could have a significant impact on the quality of water collected for water treatment for people and when getting to these places is difficult. The systematically used robot equipped with the appropriate sensors, can be part of early warning system against the pollution of water used by humans (drinking water; natural swimming pools) which can be dangerous for their health. © 2013 Koprowski et al.; licensee BioMed Central Ltd.
2012
Szymanowska-Pułka, J.; Potocka, I. W.; Karczewski, J.; Jiang, K.; Nakielski, J.; Feldman, L. J.
Principal growth directions in development of the lateral root in Arabidopsis thaliana. Journal Article
In: Annals of botany, vol. 110, no. 2, pp. 491-501, 2012, ISSN: 10958290, (9).
@article{2-s2.0-84871907416,
title = {Principal growth directions in development of the lateral root in Arabidopsis thaliana.},
author = { J. Szymanowska-Pułka and I.W. Potocka and J. Karczewski and K. Jiang and J. Nakielski and L.J. Feldman},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84871907416&doi=10.1093%2faob%2fmcs129&partnerID=40&md5=0fe95581b577a117c3987f3991ab8a9e},
doi = {10.1093/aob/mcs129},
issn = {10958290},
year = {2012},
date = {2012-01-01},
journal = {Annals of botany},
volume = {110},
number = {2},
pages = {491-501},
abstract = {During lateral root development a new meristem is formed within the mother root body. The main objective of this work was to simulate lateral root formation in Arabidopsis thaliana and to study a potential role of the principal directions in this process. Lateral root growth is anisotropic, so that three principal directions of growth can be distinguished within the organ. This suggests a tensorial character of growth and allows for its description by means of the growth tensor method. First features of the cell pattern of developing lateral roots were analysed in A. thaliana and then a tensorial model for growth and division of cells for this case was specified, assuming an unsteady character of the growth field of the organ. Microscopic observations provide evidence that the principal directions of growth are manifested at various developmental stages by oblique cell walls observed in different regions of the primordium. Other significant features observed are atypically shaped large cells at the flanks of young apices, as well as distinct boundaries between the mother root and the primordium. Simulations were performed using a model for growth. In computer-generated sequences the above-mentioned features could be identified. An attempt was made to reconstruct the virtual lateral root that included a consideration of the formation of particular tissue types based on literature data. In the cell pattern of the developing lateral root the principal directions of growth can be recognized through occurrence of oblique cell divisions. In simulation the role of these directions in cell pattern formation was confirmed, only when cells divide with respect to the principal directions can realistic results be obtained.},
note = {9},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
During lateral root development a new meristem is formed within the mother root body. The main objective of this work was to simulate lateral root formation in Arabidopsis thaliana and to study a potential role of the principal directions in this process. Lateral root growth is anisotropic, so that three principal directions of growth can be distinguished within the organ. This suggests a tensorial character of growth and allows for its description by means of the growth tensor method. First features of the cell pattern of developing lateral roots were analysed in A. thaliana and then a tensorial model for growth and division of cells for this case was specified, assuming an unsteady character of the growth field of the organ. Microscopic observations provide evidence that the principal directions of growth are manifested at various developmental stages by oblique cell walls observed in different regions of the primordium. Other significant features observed are atypically shaped large cells at the flanks of young apices, as well as distinct boundaries between the mother root and the primordium. Simulations were performed using a model for growth. In computer-generated sequences the above-mentioned features could be identified. An attempt was made to reconstruct the virtual lateral root that included a consideration of the formation of particular tissue types based on literature data. In the cell pattern of the developing lateral root the principal directions of growth can be recognized through occurrence of oblique cell divisions. In simulation the role of these directions in cell pattern formation was confirmed, only when cells divide with respect to the principal directions can realistic results be obtained.
2011
Potocka, I. W.; Szymanowska-Pułka, J.; Karczewski, J.; Nakielski, J.
Effect of mechanical stress on Zea root apex. I. Mechanical stress leads to the switch from closed to open meristem organization Journal Article
In: Journal of Experimental Botany, vol. 62, no. 13, pp. 4583-4593, 2011, ISSN: 00220957, (20).
@article{2-s2.0-80052879600,
title = {Effect of mechanical stress on Zea root apex. I. Mechanical stress leads to the switch from closed to open meristem organization},
author = { I.W. Potocka and J. Szymanowska-Pułka and J. Karczewski and J. Nakielski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052879600&doi=10.1093%2fjxb%2ferr169&partnerID=40&md5=ecf6f060f969f27f7c0019b225fc1597},
doi = {10.1093/jxb/err169},
issn = {00220957},
year = {2011},
date = {2011-01-01},
journal = {Journal of Experimental Botany},
volume = {62},
number = {13},
pages = {4583-4593},
abstract = {The effect of mechanical stress on the root apical meristem (RAM) organization of Zea mays was investigated. In the experiment performed, root apices were grown through a narrowing of either circular (variant I) or elliptical (variant II) shape. This caused a mechanical impedance distributed circumferentially or from the opposite sides in variant I and II, respectively. The maximal force exerted by the growing root in response to the impedance reached the value of 0.15 N for variant I and 0.08 N for variant II. Significant morphological and anatomical changes were observed. The changes in morphology depended on the variant and concerned diminishing and/or deformation of the cross-section of the root apex, and buckling and swelling of the root. Anatomical changes, similar in both variants, concerned transformation of the meristem from closed to open, an increase in the number of the cell layers at the pole of the root proper, and atypical oblique divisions of the root cap cells. After leaving the narrowing, a return to both typical cellular organization and morphology of the apex was observed. The results are discussed in terms of three aspects: the morphological response, the RAM reorganization, and mechanical factors. Assuming that the orientation of division walls is affected by directional cues of a tensor nature, the changes mentioned may indicate that a pattern of such cues is modified when the root apex passes through the narrowing, but its primary mode is finally restored. © 2011 The Author(s).},
note = {20},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The effect of mechanical stress on the root apical meristem (RAM) organization of Zea mays was investigated. In the experiment performed, root apices were grown through a narrowing of either circular (variant I) or elliptical (variant II) shape. This caused a mechanical impedance distributed circumferentially or from the opposite sides in variant I and II, respectively. The maximal force exerted by the growing root in response to the impedance reached the value of 0.15 N for variant I and 0.08 N for variant II. Significant morphological and anatomical changes were observed. The changes in morphology depended on the variant and concerned diminishing and/or deformation of the cross-section of the root apex, and buckling and swelling of the root. Anatomical changes, similar in both variants, concerned transformation of the meristem from closed to open, an increase in the number of the cell layers at the pole of the root proper, and atypical oblique divisions of the root cap cells. After leaving the narrowing, a return to both typical cellular organization and morphology of the apex was observed. The results are discussed in terms of three aspects: the morphological response, the RAM reorganization, and mechanical factors. Assuming that the orientation of division walls is affected by directional cues of a tensor nature, the changes mentioned may indicate that a pattern of such cues is modified when the root apex passes through the narrowing, but its primary mode is finally restored. © 2011 The Author(s).
2010
Woźnica, A.; Nowak, A.; Karczewski, J.; Kliś, C.; Bernaś, T.
Automatic biodetector of water toxicity (ABTOW) as a tool for examination of phenol and cyanide contaminated water Journal Article
In: Chemosphere, vol. 81, no. 6, pp. 767-772, 2010, ISSN: 00456535, (11).
@article{2-s2.0-77957239169,
title = {Automatic biodetector of water toxicity (ABTOW) as a tool for examination of phenol and cyanide contaminated water},
author = { A. Woźnica and A. Nowak and J. Karczewski and C. Kliś and T. Bernaś},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957239169&doi=10.1016%2fj.chemosphere.2010.07.011&partnerID=40&md5=46fe6a643fb051511c387cd40d5e4a5a},
doi = {10.1016/j.chemosphere.2010.07.011},
issn = {00456535},
year = {2010},
date = {2010-01-01},
journal = {Chemosphere},
volume = {81},
number = {6},
pages = {767-772},
publisher = {Elsevier Ltd},
abstract = {We describe an automatic biodetector for continuous monitoring of water toxicity (ABTOW). Construction of the ABTOW is based on natural ability of the biofilm formation to immobilize consortia of nitrifying bacteria (the sensing element) on the open cellular polyurethane foam as the support. Change of rates of oxygen consumption is used as an indicator of biocatalytic activity (nitrification) of the bacteria in response to xenobiotics. Owing to this design the ABTOW features stability long-term use, is inexpensive and simple in operation. The dynamics of ABTOW response is studied in details for phenol and cyanide as model toxins. These data indicate that the sensitivity was 3.5μM for phenol and 0.19μM for cyanide, respectively. The magnitudes of toxic effect were proportional to concentration whereas kinetics of the response is an indicator for the mechanism of toxicity. Similar methodology is applied to quantify toxicity of a range of heavy metals, herbicides and oxidative chain inhibitors. One may conclude that the presented biodetector provides a good sensitivity for continuous on-line monitoring of toxicity in water. © 2010 Elsevier Ltd.},
note = {11},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
We describe an automatic biodetector for continuous monitoring of water toxicity (ABTOW). Construction of the ABTOW is based on natural ability of the biofilm formation to immobilize consortia of nitrifying bacteria (the sensing element) on the open cellular polyurethane foam as the support. Change of rates of oxygen consumption is used as an indicator of biocatalytic activity (nitrification) of the bacteria in response to xenobiotics. Owing to this design the ABTOW features stability long-term use, is inexpensive and simple in operation. The dynamics of ABTOW response is studied in details for phenol and cyanide as model toxins. These data indicate that the sensitivity was 3.5μM for phenol and 0.19μM for cyanide, respectively. The magnitudes of toxic effect were proportional to concentration whereas kinetics of the response is an indicator for the mechanism of toxicity. Similar methodology is applied to quantify toxicity of a range of heavy metals, herbicides and oxidative chain inhibitors. One may conclude that the presented biodetector provides a good sensitivity for continuous on-line monitoring of toxicity in water. © 2010 Elsevier Ltd.
Woźnica, A.; Nowak, A.; Beimfohr, C.; Karczewski, J.; Bernaś, T.
Monitoring structure and activity of nitrifying bacterial biofilm in an automatic biodetector of water toxicity Journal Article
In: Chemosphere, vol. 78, no. 9, pp. 1121-1128, 2010, ISSN: 00456535, (13).
@article{2-s2.0-75449120080,
title = {Monitoring structure and activity of nitrifying bacterial biofilm in an automatic biodetector of water toxicity},
author = { A. Woźnica and A. Nowak and C. Beimfohr and J. Karczewski and T. Bernaś},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-75449120080&doi=10.1016%2fj.chemosphere.2009.12.035&partnerID=40&md5=7d9825b9b731fb593461d73a8f72643f},
doi = {10.1016/j.chemosphere.2009.12.035},
issn = {00456535},
year = {2010},
date = {2010-01-01},
journal = {Chemosphere},
volume = {78},
number = {9},
pages = {1121-1128},
publisher = {Elsevier Ltd},
abstract = {Automatic biodetector of water toxicity is a biosensor based on monitoring of catalytic activity of the nitrifying bacteria. To create a standardized biosensing system, development of the biofilm must be characterized to determine the prerequisites for its biological (biocatalytic) stability. In this paper, growth of biofilm comprising ammonium-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the open cellular polyurethane material polyurethane sponge bioreactor has been investigated. Dynamics of the biofilm formation was estimated using AOB and NOB metabolic activity and the volume occupied by these two types of bacteria in the biofilm. Spectrophotometry liquid ion chromatography and image cytometry were used, respectively, for these measurements. A mathematical model of the dynamics of biofilm formation was established. These data indicate that open cellular polyurethane material is a good basis for the immobilization of nitrifying bacteria. Moreover, growth of the biofilm leads to its stable structural form, whose biocatalytic activity (12.29 for AOB and 6.84 μmol min-1 for NOB) is constant in the long term. © 2009 Elsevier Ltd. All rights reserved.},
note = {13},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Automatic biodetector of water toxicity is a biosensor based on monitoring of catalytic activity of the nitrifying bacteria. To create a standardized biosensing system, development of the biofilm must be characterized to determine the prerequisites for its biological (biocatalytic) stability. In this paper, growth of biofilm comprising ammonium-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the open cellular polyurethane material polyurethane sponge bioreactor has been investigated. Dynamics of the biofilm formation was estimated using AOB and NOB metabolic activity and the volume occupied by these two types of bacteria in the biofilm. Spectrophotometry liquid ion chromatography and image cytometry were used, respectively, for these measurements. A mathematical model of the dynamics of biofilm formation was established. These data indicate that open cellular polyurethane material is a good basis for the immobilization of nitrifying bacteria. Moreover, growth of the biofilm leads to its stable structural form, whose biocatalytic activity (12.29 for AOB and 6.84 μmol min-1 for NOB) is constant in the long term. © 2009 Elsevier Ltd. All rights reserved.
2009
Karczewska, D. M.; Karczewski, J.; Włoch, W.; Jura-Morawiec, J.; Kojs, P.; Iqbal, M.; Krawczyszyn, J.
In: Acta Biotheoretica, vol. 57, no. 3, pp. 331-348, 2009, ISSN: 00015342, (12).
@article{2-s2.0-70350464339,
title = {Mathematical modeling of intrusive growth of fusiform initials in relation to radial growth and expanding cambial circumference in Pinus sylvestris L.},
author = { D.M. Karczewska and J. Karczewski and W. Włoch and J. Jura-Morawiec and P. Kojs and M. Iqbal and J. Krawczyszyn},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-70350464339&doi=10.1007%2fs10441-009-9068-y&partnerID=40&md5=5cbdaeba5f5744206c78a8cfc2ee3de8},
doi = {10.1007/s10441-009-9068-y},
issn = {00015342},
year = {2009},
date = {2009-01-01},
journal = {Acta Biotheoretica},
volume = {57},
number = {3},
pages = {331-348},
abstract = {This study on the cambium of Pinus sylvestris L. examines the intrusive growth of fusiform cambial initials and its possible contribution to the tangential and radial expansions of the cambial cylinder. The location and extent of intrusive growth of the fusiform initials were determined by microscopic observations and by mathematical modeling. In order to meet the required circumferential expansion of the cambial cylinder, the fusiform initials grow in groups by means of a symplastic rather than intrusive growth, leaving no room for the assumption that intrusive growth of the initials takes place between radial walls and has a direct role in the increase of the cambial circumference. Therefore, it is postulated that the fusiform initials grow intrusively between the tangential walls of the neighboring initials and their immediate derivatives and not between the radial walls of the adjacent initials as per common belief. © Springer Science+Business Media B.V. 2009.},
note = {12},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study on the cambium of Pinus sylvestris L. examines the intrusive growth of fusiform cambial initials and its possible contribution to the tangential and radial expansions of the cambial cylinder. The location and extent of intrusive growth of the fusiform initials were determined by microscopic observations and by mathematical modeling. In order to meet the required circumferential expansion of the cambial cylinder, the fusiform initials grow in groups by means of a symplastic rather than intrusive growth, leaving no room for the assumption that intrusive growth of the initials takes place between radial walls and has a direct role in the increase of the cambial circumference. Therefore, it is postulated that the fusiform initials grow intrusively between the tangential walls of the neighboring initials and their immediate derivatives and not between the radial walls of the adjacent initials as per common belief. © Springer Science+Business Media B.V. 2009.
1993
Włoch, W.; Karczewski, J.; Ogrodnik, B.
Relationship between the grain pattern in the wood, domain pattern and pattern of growth activity in the storeyed cambium of trees Journal Article
In: Trees, vol. 7, no. 3, pp. 137-143, 1993, ISSN: 09311890, (3).
@article{2-s2.0-0003451699,
title = {Relationship between the grain pattern in the wood, domain pattern and pattern of growth activity in the storeyed cambium of trees},
author = { W. Włoch and J. Karczewski and B. Ogrodnik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0003451699&doi=10.1007%2fBF00199613&partnerID=40&md5=cd31d5f0583efab6cd3ea249a2f0ad27},
doi = {10.1007/BF00199613},
issn = {09311890},
year = {1993},
date = {1993-01-01},
journal = {Trees},
volume = {7},
number = {3},
pages = {137-143},
publisher = {Springer-Verlag},
abstract = {The relationship between the arrangement of cell events occurring in cambium in a definite configuration and the grain pattern of wood was investigated. Taking into consideration the growth activity of fusiform cell ends, a model of a migrating morphogenetic wave determining an event configuration was made. Waves of length λ=1 m for the periods T=2 years and T=3 years and waves of lengths λ=l m and λ=0.04 m for the period T=10 years were considered. On the model, events from successive annual rings, conventionally comprising 10 cell layers each, were summed. In this way, event maps were obtained. For wave λ=4 mm, the domain pattern on the modelled map was compatible with the grain pattern. The domain pattern for the wave λ=1 m was impossible to recreate because the wave "migrated too fast". In this case, the pattern of event configuration, incompatible with the grain pattern, formed microareas, which were not domains. © 1993 Springer-Verlag.},
note = {3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The relationship between the arrangement of cell events occurring in cambium in a definite configuration and the grain pattern of wood was investigated. Taking into consideration the growth activity of fusiform cell ends, a model of a migrating morphogenetic wave determining an event configuration was made. Waves of length λ=1 m for the periods T=2 years and T=3 years and waves of lengths λ=l m and λ=0.04 m for the period T=10 years were considered. On the model, events from successive annual rings, conventionally comprising 10 cell layers each, were summed. In this way, event maps were obtained. For wave λ=4 mm, the domain pattern on the modelled map was compatible with the grain pattern. The domain pattern for the wave λ=1 m was impossible to recreate because the wave "migrated too fast". In this case, the pattern of event configuration, incompatible with the grain pattern, formed microareas, which were not domains. © 1993 Springer-Verlag.